Acute Toxicity Study Of Canine Mesenchymal Stem Cell (Msc)-Derived Exosome Preparation For Drug Registration,
2024
Faculty of Pharmaceutical Sciences
Acute Toxicity Study Of Canine Mesenchymal Stem Cell (Msc)-Derived Exosome Preparation For Drug Registration, Anatcha Thongsit
Chulalongkorn University Theses and Dissertations (Chula ETD)
Exosomes, spherical vesicles secreted by cells containing diverse biomolecules, are gaining attention in veterinary medicine. Mesenchymal stem cell (MSC)-derived exosomes show promise for treating animal diseases. However, their safety profile, particularly the acute toxicity of canine MSC-derived exosomes, has yet to be adequately explored. This study evaluates the acute toxicity of canine MSC-derived exosomes on rat systemic health, adhering to ISO 10993-11 guidelines for systemic toxicity testing to support veterinary drug registration and development. The study tested acute toxicity in five female Sprague Dawley rats by administering exosomes at a concentration of 1×109 particles/mL via intravenous injection into the tail …
Expression Optimization And Characterization Of Plant-Produced Human Interleukin-15,
2024
Faculty of Pharmaceutical Sciences
Expression Optimization And Characterization Of Plant-Produced Human Interleukin-15, Chalatorn Charnsatabut
Chulalongkorn University Theses and Dissertations (Chula ETD)
Human interleukin-15 (hIL-15) is a cytokine that plays a crucial role in immune responses like T cell proliferation, activation, and prolongation of natural killer (NK) cells. This cytokine also impacts the cell proliferation of human adult low calcium temperature (HaCaT) keratinocytes which can lead to the wound-healing effect. This study aimed to optimize the expression and characterize the recombinant hIL-15 in Nicotiana benthamiana with the Agrobacterium-mediated transient expression system. The hIL-15 gene was fused with the Fc domain from the IgG1 antibody for purification. The expression cassette was optimized for sub-cellular targeting and agroinfiltration. The transiently expressed hIL-15-Fc was purified …
Molecular Mechanism Of Lusianthridin On Platelet Aggregation Inhibition And Endothelial Cell Protection,
2024
Faculty of Pharmaceutical Sciences
Molecular Mechanism Of Lusianthridin On Platelet Aggregation Inhibition And Endothelial Cell Protection, Fozia -
Chulalongkorn University Theses and Dissertations (Chula ETD)
Lusianthridin (LST), a bioactive compound isolated from Dendrobium venustum, exhibits notable vasoprotective properties. This study aimed to elucidate the underlying molecular mechanisms of LST by evaluating its effects on platelet function and endothelial cell activation in vitro. In human platelets, LST significantly inhibited collagen-induced aggregation, an effect associated with elevated phosphorylation of vasodilator-stimulated phosphoprotein (VASP) and suppression of Akt phosphorylation. In EA.hy926 endothelial cells, LST attenuated tumor necrosis factor-alpha (TNF-α)-induced endothelial dysfunction by reducing nitric oxide (NO) production and suppressing the expression of pro-inflammatory cytokines, including interleukin-6 (IL-6) and interleukin-1β (IL-1β). Mechanistic investigations revealed that LST inhibited key inflammatory signaling …
Predicting The Eq-5d-5l Utility Scores From The Impact Of Vision Impairment (Ivi) Questionnaire In Thai Patients,
2024
Faculty of Pharmaceutical Sciences
Predicting The Eq-5d-5l Utility Scores From The Impact Of Vision Impairment (Ivi) Questionnaire In Thai Patients, Emilia Audrey Tunmas Pasenggong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Objectives : (1) This study aims to predict the EQ-5D-5L utility scores from the impact of vision impairment (IVI) questionnaire in Thai patients using mapping techniques. (2) To investigate the association between sociodemographic and clinical variables with the EQ-5D-5L index scores. Methods : This is a secondary data analysis. A total of 499 patients with multiple levels of visual impairment were recruited from King Chulalongkorn Memorial Hospital Thailand between February and July 2022. Ordinary least square (OLS), Tobit, Censored least absolute deviation (CLAD), and Adjusted limited dependent variable mixture model (ALDVMM) regression models were used to map the IVI questionnaire …
Development Of Granule Product Containing Curcuminoid Extract And Hyaluronic Acid Derivative For Oral Administration,
2024
Faculty of Pharmaceutical Sciences
Development Of Granule Product Containing Curcuminoid Extract And Hyaluronic Acid Derivative For Oral Administration, Natkhanang Boonpetcharat
Chulalongkorn University Theses and Dissertations (Chula ETD)
Curcumin is widely recognized for its various pharmacological properties, including antioxidant, anti-inflammatory, and anti-tumor activities. Nevertheless, the development of curcumin as a therapeutic agent is impeded by its limited oral bioavailability, which stems from its chemical instability, poor aqueous solubility, and rapid degradation. This study aimed to develop granule formulations incorporating hyaluronic acid graft poly(N-isopropylacrylamide) or HA-g-pNIPAM to enhance dissolution and protect curcumin from degradation. Three formulations were developed: F10 (HA-g-pNIPAM physically mixed with curcumin), F10 Encap (curcumin encapsulated within HA-g-pNIPAM), and F11 (curcumin granules without HA-g-pNIPAM). The stability results showed that F10 Encap effectively maintained curcumin content throughout the …
The Effect Of Stilbene Derivatives On Modulation Of P-Gp Efflux Transporter,
2024
Faculty of Pharmaceutical Sciences
The Effect Of Stilbene Derivatives On Modulation Of P-Gp Efflux Transporter, Shahnila Mukhtar
Chulalongkorn University Theses and Dissertations (Chula ETD)
Drug efflux by transporters of the ATP-binding cassette (ABC) family such as P-glycoprotein is the earliest and most well-studied multidrug resistant (MDR) mechanism. Overexpression of ATP-binding cassette transporters can decrease the effectiveness of chemotherapy by pumping anti-cancer drugs out of cancer cells. This study was to predict the best-fit of certain stilbenoids isolated from Thai orchids as a specific P-gp moderator, using the molecular docking approach to compare and specify the binding interaction between each stilbenoids and the P-gp binding domains. Furthermore, the in vitro substrate uptake assays of eight stilbenoids with phenanthrene core structure were conducted for assessing their …
Factors Contributing To Self-Medication And Quality Of Life Of Myanmar Immigrants In The Covid-19 Pandemic Situation,
2024
Faculty of Pharmaceutical Sciences
Factors Contributing To Self-Medication And Quality Of Life Of Myanmar Immigrants In The Covid-19 Pandemic Situation, Su Myat Thin
Chulalongkorn University Theses and Dissertations (Chula ETD)
Background: Due to numerous difficulties in accessing necessary healthcare and standard medication, immigrants might be more likely to rely on self-medication to solve health problems they suffered. Although it might provide immediate relief, it could also bring some risks such as adverse drug reactions, antimicrobial resistance progression, drug side effects, and toxicity. This study aimed to identify the healthcare service accessibility and self-medication of immigrants in Thailand for COVID-19. Additionally, this study also aimed to evaluate the effect of immigrants’ COVID-19 perception on their self-medication uses and quality of life. This study had two phases in which the first phase …
The Effect Of Artogomezianone On Apoptosis Induction In Non-Small-Cell Lung Cancer,
2024
Faculty of Pharmaceutical Sciences
The Effect Of Artogomezianone On Apoptosis Induction In Non-Small-Cell Lung Cancer, Worawich Somboonvoravong
Chulalongkorn University Theses and Dissertations (Chula ETD)
NSCLC severity is driven by dysregulation of the EGFR/PI3K/Akt pathway, promoting tumor growth and drug resistance. Hyperactive EGFR/PI3K/Akt signaling results in aggressive tumors and poor outcomes, further worsened by resistance to EGFR inhibitors. Targeting this pathway could inhibit tumor growth and resistance. artogomezianone, a compound derived from Artocarpus gomezianus, has shown considerable anti-cancer efficacy, mainly by inducing apoptosis and decreasing the proliferation of several cancers. This study investigated artogomezianone’s effects on the EGFR/PI3K/Akt pathway. Cytotoxic and apoptotic effects were assessed using MTT assay and Hoechst 33342/PI double staining, with confirmation by Western blot analysis. Protein levels and binding affinity were …
Role Of Edaravone Nanosuspension In Preterm Labor And Birth Outcomes,
2024
Saint John's University, Jamaica New York
Role Of Edaravone Nanosuspension In Preterm Labor And Birth Outcomes, Sruthi Sarvepalli
Theses and Dissertations
Annually, preterm birth (PTB) affects fifteen million infants, posing a significant risk of infant mortality and lifelong disability. Among various triggers, it is well-established that infection-associated oxidative stress and inflammation can induce preterm labor. There is no FDA approved medication for the prevention of PTB except tocolytics which provide marginal benefit at a later stage. Scarcity of therapeutic intervention and unmet medical need propelled us in investigating a novel drug molecule. Our current research is focused on investigating the potential of Edaravone (EDA), an FDA-approved antioxidant and anti-inflammatory medication, in delaying preterm labor and improving neonatal outcomes. Our goal is …
In Silico Identifcation Of New Potential Inhibitors Of Quorum Sensing By Gram‑Positive Bacteria Through Specialized Molecular Docking,
2024
The British University in Egypt
In Silico Identifcation Of New Potential Inhibitors Of Quorum Sensing By Gram‑Positive Bacteria Through Specialized Molecular Docking, Amgad Albohy, Najla A. Obaid, Mariam Mojally, Mohammed Abou Rehab, Najd Ahmad Alkhudhir
Pharmacy
Quorum sensing is the process by which bacterial cells can communicate by producing substances to regulate viable processes such as gene expression, virulence, and bioflm formation. Gram-positive bacteria such as Staphylococcus aureus and Enterococcus faecalis have specifc enzymes (autoinducers) that control the quorum sensing system. Sortase A is a surface protein that regulates virulence and cell‒cell communication in Gram-positive bacteria. To interfere with this system and reduce virulence and cell‒cell communication, quorum sensing inhibitors are used, which are nonantibiotic substances. In this study, we aimed to use Food and Drug Administration-approved drugs (analgesics and antipsychotics) and investigate their activity using …
Development Of New Radiotracers For Positron Emission Tomography Targeting C-X-C Chemokine Receptor Type 4 In Cancers,
2024
Faculty of Pharmaceutical Sciences
Development Of New Radiotracers For Positron Emission Tomography Targeting C-X-C Chemokine Receptor Type 4 In Cancers, Piyapan Suwattananuruk
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study aimed to develop novel positron emission tomography (PET) tracers for C-X-C chemokine receptor type 4 (CXCR4) imaging. [68Ga]Ga-TD-01 and [18F]PICO-F were designed as PET tracers based on the molecular scaffolds of two potent CXCR4 antagonists, TIQ15 and PICO. TIQ15 was used to prepare a bifunctional (BFC) to facilitate 68Ga-labeling while structural modification of PICO was made to obtain a precursor, PICO-Br for 18F-labeling. The BFC, TD-01 was obtained in a moderately high yield (61%) via the conjugation of TIQ15 with a chelator derivative and successfully radiolabeled with 68Ga to yield [68Ga]Ga-TD-01, a PET tracer. For 18F-based tracer, the …
Scaffold Hopping For The Lead Optimization Of The Orally Active Antileishmanial Pyrrolo-Quinazoline Alkaloid Natural Product Peganine,
2024
South Dakota State University
Scaffold Hopping For The Lead Optimization Of The Orally Active Antileishmanial Pyrrolo-Quinazoline Alkaloid Natural Product Peganine, Sunitha Jada
Electronic Theses and Dissertations
Leishmaniasis is a protozoan parasitic disease caused by the species Leishmania that affects millions of people around the world. It is a serious public health concern in the developing world with about 1 million cases and 70,000 fatalities occurring each year. It has several forms with visceral, and cutaneous leishmaniasis being the most common forms of the disease. Visceral leishmaniasis, caused by the Leishmania donovani occurs mainly in Brazil, Eastern Africa, and India, and is fatal if left untreated. The current treatments for leishmaniasis such as pentavalent antimonials, amphotericin B, miltefosine, paromomycin, pentamidine, and some azoles suffer from serious drawbacks. …
Application Of Interpretable Machine Learning Methods To Study The Disease Characteristics And Healthcare Expenditures In Hodgkin’S Lymphoma,
2024
West Virginia University
Application Of Interpretable Machine Learning Methods To Study The Disease Characteristics And Healthcare Expenditures In Hodgkin’S Lymphoma, Zasim Azhar Dil Hasan Siddiqui
Graduate Theses, Dissertations, and Problem Reports (ETD)
Hodgkin’s lymphoma (HL) is a rare malignancy of lymphocytes that predominantly occurs in young adults aged 20-30 years or elderly individuals aged 65-75 years. Despite its low incidence, there were an estimated 223,512 HL survivors in the US in 2020. Hodgkin’s lymphoma shows a favorable prognosis among young adults, with a high cure rate of 85-90%; however, older adults experience poor prognosis, with a 5-year overall survival rate of 40-55% in patients over 60 years. HL survivors incur high total and out-of-pocket (OOP) healthcare expenditures, averaging $78,183 and $4,180 per patient in the first year after diagnosis, highlighting a considerable …
4d Epr Oximetry Imaging For 4d Bioprinting And Tissue Engineering,
2024
West Virginia University
4d Epr Oximetry Imaging For 4d Bioprinting And Tissue Engineering, Sajad Sarvari
Graduate Theses, Dissertations, and Problem Reports (ETD)
Bioprinting, akin to 3D printing, employs cell-laden hydrogels such as GelMA (Gelatine Methacrylate) and Alginate instead of plastic or resin to create biological structures. The printing process followed by subsequent tissue maturation is known as 4D bioprinting, where the fourth dimension is time. Ensuring adequate and consistent oxygen, O2, supply within 3D bioprint volume over the entire maturation period process is crucial, as even transient hypoxia can impact cellular behavior and phenotypes. However, printing of fully developed vasculature remains an unresolved technical challenge. Several approaches to chemically (peroxides) or biologically (photosynthesis) generate oxygen during bioprint maturation have been proposed. However, …
Advancements In The Synthesis Of Polyoxygenated Oxepanes And Thiepanes For Applications To Natural Products,
2024
AstraZeneca PLC
Advancements In The Synthesis Of Polyoxygenated Oxepanes And Thiepanes For Applications To Natural Products, Aditya R. Pote, Shayne M. Weierbach, Mark W. Peczuh, Kyle M. Lambert
Chemistry & Biochemistry Faculty Publications
Oxepanes are central motifs and tenants of many biologically important molecules, and their synthetic construction often presents a challenge to chemists due to consequential entropic and enthalpic barriers that have limited the synthetic toolbox to access these seven-membered oxacycles. This review covers the breadth of synthetic methods to afford the oxepane/thiepane moiety, with a focus on polyoxygenated oxepanes and includes radical cyclizations, Lewis acid-mediated cyclizations, ring closing-metathesis, Nicholas-Ferrier rearrangement, homologations, and ring-expansion strategies. Implementation of these tactics towards sugar-based and non-sugar based (de novo) approaches is presented alongside their extensive application to the total synthesis of several complex polyoxygenated oxepane-containing …
Research News, Publications, 2024, Volume 2,
2024
School of Pharmacy, University of Mississippi
Research News, Publications, 2024, Volume 2, Soumyajit Majumdar
Publications (2015-)
Publications that appeared during the period of April 1 through June 30, 2024.
Research News, Publications, 2024, Volume 3,
2024
School of Pharmacy, University of Mississippi
Research News, Publications, 2024, Volume 3, Soumyajit Majumdar
Publications (2015-)
Publications that appeared during the period of July 1 through September 30, 2024.
Research News, Publications, 2024, Volume 4,
2024
School of Pharmacy, University of Mississippi
Research News, Publications, 2024, Volume 4, Joshua Sharp
Publications (2015-)
Publications that appeared during the period of October 1 - December 31, 2024.
Research News, Grants, 2024, Volume 3,
2024
School of Pharmacy, University of Mississippi
Research News, Grants, 2024, Volume 3, Soumyajit Majumdar
Grants (2015-)
Grants and contracts awarded during July - September 2024 - Revised 10/22/24
Research News, Grants, 2024, Volume 4,
2024
School of Pharmacy, University of Mississippi
Research News, Grants, 2024, Volume 4, Joshua Sharp
Grants (2015-)
Grants and contracts awarded during October - December 2024
