Plant Breeding and Genetics Commons^™

Modifications Of Membrane Lipids In Response To Wounding Of Arabidopsis Thaliana Leaves, Hieu Sy Vu, Rebecca Roston, Sunitha Shiva, Manhoi Hur, Eve Syrkin Wurtele, Xuemin Wang, Jyoti Shah, Ruth Welti

Center for Plant Science Innovation: Faculty Publications

Mechanical wounding of Arabidopsis thaliana leaves results in modifications of most membrane lipids within 6 hours. Here, we discuss the lipid changes, their underlying biochemistry, and possible relationships among activated pathways. New evidence is presented supporting the role of the processive galactosylating enzyme SENSITIVE TO FREEZING2 in the wounding response.

The Taurine Biosynthetic Pathway Of Microalgae, Rahul Tevatia, James Allen, Deepak Rudrappa, Derrick White, Thomas E. Clemente, Heriberto Cerutti, Yaşar Demirel, Paul H. Blum

Center for Plant Science Innovation: Faculty Publications

Taurine (2-aminoethanesulfonic acid) is an amino acid–like compound widely distributed in animals and an essential nutrient in some species. Targeted metabolomics of marine and freshwater microalgae combined with medium supplementation identified biosynthetic pathway intermediates and necessary catalytic activities. Genomic analysis was then used to predict the first taurine biosynthetic pathway in these organisms. MRM-based electrospray ionization (ESI) LC–MS/MS analysis demonstrated that taurine is synthesized using a carbon backbone from L-serine combined with sulfur derived from sulfate. Metabolite analysis showed a nonuniform pattern in levels of pathway intermediates that were both species and supplement dependent. While increased culture salinity raised taurine …

Sugar-Enhanced And Synergistic Sweet Corn Cultivar Evaluation For Northern Indiana, 2014, Elizabeth Maynard

Midwest Vegetable Trial Reports

Indiana sweet corn acreage harvested for fresh market averaged 5,233 acres annually from 2011- 2013, with a yield of 63 hundredweight per acre (149 crates or 3.1 tons per acre) and an annual value of $13.9 million (USDA NASS, 2014).

Sweet corn fields for fresh market sales are located throughout the state. In northern Indiana, bicolor corn is most commonly grown. Varieties with improved eating quality are of interest to both producers and consumers. Producers are also interested in yield, ear size, appearance, and agronomic characteristics.

This paper reports on nine bicolor and one yellow sugar-enhanced or synergistic sweet corn …

Supersweet Sweet Corn Cultivar Evaluation For Northern Indiana, 2014, Elizabeth Maynard

Midwest Vegetable Trial Reports

Indiana sweet corn acreage harvested for fresh market averaged 5,233 acres annually from 2011- 2013, with a yield of 63 hundreweight per acre (149 crates or 3.1 tons per acre) and an annual value of $13.9 million (USDA NASS, 2014). Sweet corn fields for fresh market sales are located throughout the state. In northern Indiana, bicolor corn is most commonly grown.

Varieties with improved eating quality are of interest to both producers and consumers. The term ‘supersweet’ commonly refers to sweet corn with two copies of the shrunken-2 (sh2) gene that have high levels of sugar in the kernels and …

Midwest Vegetable Trial Report For 2014, Elizabeth Maynard

Midwest Vegetable Trial Reports

This is a compilation of 30 research trial reports from seven land-grant universities in the midwestern and northeastern United States. Crops covered include Asian vegetables (Napa cabbage, pak choy or bok choi, mizuna, tatsoi, Yukina savoy, komatsuna, senposai, and Tokyo bekana) broccoli, cantaloupe, cucumber, kohlrabi, pepper, potato, pumpkin, radish, sweet corn, tomato, and watermelon. Several crops were evaluated in high tunnels or hoophouses. Most trials compared different cultivars or varieties. Four reports addressed specific growing practices: the use of hairy vetch to supply nitrogen, biochar use in a high tunnel bag culture system, effects of organic soil amendments, and scion-rootstock …

Nutrient Competition Between Algae And Juncus Effusus In The Lake Fayetteville Artificial Spiral Wetland, Toryn Jones, Thad Scott

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

There is insufficient research focusing exclusively on how nutrient competition between algae and wetland macrophytes affects the growth of these species. This study examined the relationship between nutrient concentrations (N and P), algal concentrations, and the growth of Juncus effusus. Juncus effusus growth in the Lake Fayetteville artificial spiral wetland was monitored over a four month period during the prime growing season. Eighteen plants were taken from the wetland and replanted in 1 of 6 treatments: plant-only, algae-only, combined, plant-only +supplement, algaeonly +supplement, or combined +supplement. The algae and combined environments received an inoculation of algae, and the +supplement treatments …

Genetic And Functional Analysis Of Host Genes Involved In Pathogenic And Symbiotic Legume-Microbe Interactions, Fang Tang

Theses and Dissertations--Plant and Soil Sciences

Legumes form symbiotic and pathogenic interactions with microbes. Understanding the molecular mechanisms underlying the legume-microbe interactions would help us to improve crop production in a sustainable manner. This thesis covers two independent research projects. The first project was to study the role of alternative splicing in RCT1-mediated disease resistance. RCT1 is a TIR-NBS-LRR-type plant resistance (R) gene in Medicago truncatula that confers broad-spectrum resistance to Colletotrichum trifolii, a fungal pathogen that causes anthracnose disease in Medicago. RCT1 undergoes alternative splicing at both coding and 3'-untranslated regions, thereby producing multiple transcript variants in its expression profile. …

Selection And Basis For 2,4-D (2,4-Dicholorphenoxyacetic Acid) Tolerance In Red Clover (Trifolium Pratense), Tara L. B. Lewis

Theses and Dissertations--Plant and Soil Sciences

A red clover (Trifolium pratense) population (UK), from a cross between the cultivar Kenland and a 2,4-D tolerant population (Florida), was recurrently selected for 2,4-D tolerance with evaluations after the 6^th, 7^th, and 8^th selection cycles. All UK populations were more 2,4-D tolerant than Kenland. The 2,4-D tolerance following the 6^th selection cycle was similar to the Florida population and tolerance was increased following 7 and 8 cycles of selection by removing plants showing 2,4-D injury and doubling the rate of 2,4-D used for selection.

Yield and forage quality were evaluated in …

Supersweet Sweet Corn Cultivar Evaluation For Northern Indiana, 2014, Elizabeth Maynard

Purdue Fruit and Vegetable Research Reports

Indiana sweet corn acreage harvested for fresh market averaged 5,233 acres annually from 2011- 2013, with a yield of 63 hundreweight per acre (149 crates or 3.1 tons per acre) and an annual value of $13.9 million (USDA NASS, 2014). Sweet corn fields for fresh market sales are located throughout the state. In northern Indiana, bicolor corn is most commonly grown.

Varieties with improved eating quality are of interest to both producers and consumers. The term ‘supersweet’ commonly refers to sweet corn with two copies of the shrunken-2 (sh2) gene that have high levels of sugar in the kernels and …

Sugar-Enhanced And Synergistic Sweet Corn Cultivar Evaluation For Northern Indiana, 2014, Elizabeth Maynard

Purdue Fruit and Vegetable Research Reports

Indiana sweet corn acreage harvested for fresh market averaged 5,233 acres annually from 2011- 2013, with a yield of 63 hundredweight per acre (149 crates or 3.1 tons per acre) and an annual value of $13.9 million (USDA NASS, 2014).

Sweet corn fields for fresh market sales are located throughout the state. In northern Indiana, bicolor corn is most commonly grown. Varieties with improved eating quality are of interest to both producers and consumers. Producers are also interested in yield, ear size, appearance, and agronomic characteristics.

This paper reports on nine bicolor and one yellow sugar-enhanced or synergistic sweet corn …

Midwest Vegetable Trial Report For 2014, Elizabeth Maynard

Purdue Fruit and Vegetable Research Reports

This is a compilation of 30 research trial reports from seven land-grant universities in the midwestern and northeastern United States. Crops covered include Asian vegetables (Napa cabbage, pak choy or bok choi, mizuna, tatsoi, Yukina savoy, komatsuna, senposai, and Tokyo bekana) broccoli, cantaloupe, cucumber, kohlrabi, pepper, potato, pumpkin, radish, sweet corn, tomato, and watermelon. Several crops were evaluated in high tunnels or hoophouses. Most trials compared different cultivars or varieties. Four reports addressed specific growing practices: the use of hairy vetch to supply nitrogen, biochar use in a high tunnel bag culture system, effects of organic soil amendments, and scion-rootstock …

Examining Vegetative Growth Of Cool-Season Forage Grasses For Dairy Cattle Grazing Preference, Eric D. Billman

Theses and Dissertations--Plant and Soil Sciences

The objective of this study was to determine dairy cattle preference amongst four species of cool-season forage grasses: eight orchardgrasses (Dactylis glomerata L.), five tall fescues [Schedonorus arundinaceus (Schreb.) Dumort.], five perennial ryegrasses (Lolium perenne L.), and six festuloliums [xFestulolium braunii (K. Richt.) A. Camus.]; 24 cultivars in total. Each grazing trial utilized four Holstein-Friesian heifers over six hours. Maturity differences were eliminated by having animals graze only vegetative material. After six grazing trials (three each in 2014 and 2015), consistent results in animal preference were not found; three of the six trials did show preference …

Generation Of An Attenuated, Cross-Protective Pepino Mosaic Virus Variant Through Alignment-Guided Mutagenesis Of The Viral Capsid Protein, Godwill M. Chewachong, Sally A. Miller, Joshua J. Blakeslee, David M. Francis, Thomas Jack Morris, Feng Qu

School of Biological Sciences: Faculty Publications

Mild variants of many viruses are able to protect infected plants from subsequent invasion by more severe variants of the same viruses through a process known as cross-protection. In the past, the cross-protective viral variants were commonly derived from mild field isolates that were sometimes genetically heterogeneous, providing variable levels of crossprotection. Here, we report a novel approach to rapidly generate crossprotective variants of the tomato-infecting Pepino mosaic virus (PepMV) independently of the availability of mild field isolates. Our approach sought to attenuate PepMV by mutating less conserved amino acid residues of the abundantly produced capsid protein (CP). These less-conserved …

Breeding For Nitrogen Use Efficiency In Soft Red Winter Wheat, Katlyn Hitz

Theses and Dissertations--Plant and Soil Sciences

Nitrogen use efficient (NUE) wheat varieties have potential to reduce input costs for growers, limit N runoff into water ways, and increase wheat adaptability to warmer environments. Previous studies have done little to explain the genetic basis for NUE and components, nitrogen uptake efficiency (NUpE) and nitrogen utilization efficiency (NUtE). Four studies were conducted to 1) determine genotypic stability of NUE under high and low N regimes and under warming 2) determine effect of warming on NUE 3) indentify QTL associated with NUE components 4) assess the utility of canopy spectral reflectance (CSR) as a high-throughput phenotyping device for NUE. …

Germplasm Enhancement For Resistance To Pyrenophora Tritici-Repentis In Wheat, Manisha Shankar, Diane E. Mather, Dorthe Jorgensen, Hossein Golzar, Ken J. Chalmers, Grant J. Hollaway, Mark S. Mclean, Stephen M. Neate, Rob Loughman

Conference papers and presentations

Yellow spot (syn. tan spot), caused by Pyrenophora tritici-repentis, is an important foliar disease of wheat in Australia that causes losses exceeding 50 % when conditions are favourable for disease development. Although good progress has been made internationally to understand yellow spot resistance, relatively few resistance genes have been identified and mapped in Australian germplasm and only one (tsn1 on chromosome 5BL) is in general and known use in Australian breeding programs. Although tsn1 is an important yellow spot resistance gene, it doesn’t explain the full spectrum of resistance and there is a significant opportunity to enhance expression …

Characterization Of A Novel Fungal-Specific Gene, Fug1, In Fusarium Verticillioides, John Byron Ridenour

Graduate Theses and Dissertations

Filamentous fungi are responsible for numerous plant and animal diseases. The filamentous ascomycete Fusarium verticillioides is a globally important pathogen of maize, capable of causing severe yield reductions and economic losses. Also of substantial concern is the contamination of infected kernels with fumonisins, toxic secondary metabolites linked to toxicoses in humans and livestock. The number of sequenced fungal genomes is rapidly increasing. However, functional characterization of fungal genes has not progressed at a comparable rate. In pathogenic fungi, uncharacterized genes represent a source for novel virulence factors or anti-fungal targets. Therefore, to fully understand the genetic mechanisms underlying fungal pathogenesis, …

Carbohydrate Metabolism And The Trehalose Biosynthetic Pathway In Maize Kernels Grown In Vitro Under Sucrose Starvation Stress, Samuel W. Bledsoe

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Drought is an increasing issue that many farmers encounter especially in hot arid climates with little rainfall. High temperatures and inadequate rainfall at certain stages in crop development can have disastrous consequences to yield. In maize, drought occurring near or during the flowering stage often causes significant kernel abortion that greatly impacts potential yield. The trehalose biosynthetic pathway has recently been found to be important in plant metabolism in response to stress in higher order plants. Trehalose is currently known throughout the plant and animal kingdoms as an osmoprotectant, high energy fuel source, structural component, and involved in pathogen response. …

European Consumers' Attitudes Towards Cisgenic Rice, Anne-Cécile Delwaide

Graduate Theses and Dissertations

The enhancement of existing plant breeding techniques, such as cisgenesis, allows plant breeders to enhance an existing cultivar quicker and with little to no genetic drag. Cisgenesis is the genetic modification of a recipient plant with natural gene(s) from a sexually compatible plant. Unlike transgenesis, which is the genetic modification of a recipient plant with gene(s) from any non‐plant organism, or from a donor plant that is sexually incompatible with the recipient plant, the results of cisgenesis could occur naturally over time. Currently, both cisgenic and transgenic products are classified as genetically modified organisms (GMOs) and are labeled as such …

2015 Spring Seed Guide, Teshome Regassa, Charles A. Shapiro

Department of Agronomy and Horticulture: Faculty Publications

NEBRASKA CORN HYBRID TESTS CROP PRODUCTION SUMMARY: According to the National Agricultural Statistics Service, there were 8.75 million acres of corn harvested in Nebraska in 2014 producing approximately 1.58 billion bushels of grain. The total average corn yield for Nebraska in 2014 was a record 181 bushels per acre (bu/a). Total corn yields from the previous 10 years are reported below.

NEBRASKA SOYBEAN VARIETY TESTS - 2014 - CROP PRODUCTION SUMMARY: According to the National Agricultural Statistics Service, there were 5.4 million acres of soybeans planted in Nebraska in 2014. 5.35 million acres were harvested producing around 288 million bushels. …

Consumer Acceptance And Willingness To Pay For Genetically Modified Rice In China: A Double Bounded Dichotomous Choic Contingent Valuation Survey Calibrated By Cheap Talk, Jing Jin

Graduate Theses and Dissertations

Considering population growth, limitations on land and water resources, and contamination to the ecosystem due to agricultural activities, current rice production in China is facing pressure to fulfill national demand. Self-sufficiency of rice has been a long-held political objective of the Chinese government and it is national goal to maintain the equilibrium between the national production and consumption or even achieve a supply surplus in rice. With the developing bio-technology of genetic modification (GM), scientists believe that using genetically modified cultivars may ease the pressure mentioned above. However, both the government and the people are very cautious about large-scale cultivation …