Botany Commons^™

The Heterogeneity In The Landscape Of Gene Dominance In Maize Is Accompanied By Unique Chromatin Environments, Liangwei Yin, Gen Xu, Jinliang Yang, Meixia Zhao

Department of Agronomy and Horticulture: Faculty Publications

Subgenome dominance after whole-genome duplication (WGD) has been observed in many plant species. However, the degree to which the chromatin environment affects this bias has not been explored. Here, we compared the dominant subgenome (maize1) and the recessive subgenome (maize2) with respect to patterns of sequence substitutions, genes expression, transposable element accumulation, small interfering RNAs, DNA methylation, histone modifications, and accessible chromatin regions (ACRs). Our data show that the degree of bias between subgenomes for all the measured variables does not vary significantly when both of the WGD genes are located in pericentromeric regions. Our data further indicate that the …

Next-Generation Technologies Unlock New Possibilities To Track Rangeland Productivity And Quantify Multi-Scale Conservation Outcomes, Caleb P. Roberts, David E. Naugle, Brady W. Allred, Victoria M. Donovan, Dillon T. Fogarty, Matthew O. Jones, Jeremy D. Maestas, Andrew C. Olsen, Dirac L. Twidwell Jr

Department of Agronomy and Horticulture: Faculty Publications

Historically, relying on plot-level inventories impeded our ability to quantify large-scale change in plant biomass, a key indicator of conservation practice outcomes in rangeland systems. Recent technological advances enable assessment at scales appropriate to inform management by providing spatially comprehensive estimates of productivity that are partitioned by plant functional group across all contiguous US rangelands. We partnered with the Sage Grouse and Lesser Prairie-Chicken Initiatives and the Nebraska Natural Legacy Project to demonstrate the ability of these new datasets to quantify multi-scale changes and heterogeneity in plant biomass following mechanical tree removal, prescribed fire, and prescribed grazing. In Oregon’s sagebrush …

Cytogenetic And Genomic Characterization Of A Novel Tall Wheatgrass‑Derived Fhb7 Allele Integrated Into Wheat B Genome, Wei Zhang, Tatiana Danilova, Mingyi Zhang, Shuangfeng Ren, Xianwen Zhu, Qijun Zhang, Shaobin Zhong, Linda Dykes, Jason Fiedler, Steven Xu, Katherine Frels, Stephen Wegulo, Jeffrey Boehm Jr., Xiwen Cai

Department of Agronomy and Horticulture: Faculty Publications

A novel tall wheatgrass-derived (Thinopyrum elongatum, genome EE) Fhb7 allele, designated Fhb7^The2, was identified and integrated into the wheat B genome through a small 7B–7E translocation (7BS·7BL–7EL) involving the terminal regions of the long arms. Fhb7^The2 conditions significant Type II resistance to Fusarium head blight (FHB) in wheat. Integration of Fhb7^The2 into the wheat B genome makes this wild species-derived FHB resistance gene usable for breeding in both common and durum wheat. By contrast, other Fhb7 introgression lines involving wheat chromosome 7D can be utilized only in common wheat breeding programs, not in durum …

How Digital Is Agriculture In A Subset Of Countries From South America? Adoption And Limitations, Laila A. Puntel, É. L. Bolfe, R. J. M. Melchiori, R. Ortega, G. Tiscornia, A. Roel, F. Scaramuzza, S. Best, A. G. Berger, D. S. S. Hansel, D. Palacios Durán, G. R. Balboa

Department of Agronomy and Horticulture: Faculty Publications

Digital agriculture (DA) can contribute solutions to meet an increase in healthy, nutritious, and affordable food demands in an efficient and sustainable way. South America (SA) is one of the main grain and protein producers in the world but the status of DA in the region is unknown. A systematic review and case studies from Brazil, Argentina, Uruguay, and Chile were conducted to address the following objectives: (1) quantify adoption of existing DA technologies, (2) identify limitations for DA adoption; and (3) summarise existing metrics to benchmark DA benefits. Level of DA adoption was led by Brazil and Argentina followed …

Cropland Reference Ecological Unit: A Land Classification Unit For Comparative Soil Studies, Saurav Das, Bijesh Maharjan

Department of Agronomy and Horticulture: Faculty Publications

There is a growing consensus on a need for measuring the dynamic soil properties of croplands and even comparisons with a reference state or native land. These measurements and paired comparisons will create the capacity to determine soil health management effects and targets. However, the complex soil heterogeneity and climate variations make soil health potential variable and confound the effects of land-use and management practices and comparisons between soils from different sites. Identifying a discrete landmass unit where all soils have similar health potential will be critical in conducting meaningful comparative studies and measuring the impact of conservation practices. This …

Cell-Type-Specific Profiling Of The Arabidopsis Thaliana Membrane Protein-Encoding Genes, Sergio Alan Cervantes-Pérez, Marc Libault

Department of Agronomy and Horticulture: Faculty Publications

Membrane proteins work in large complexes to perceive and transduce external signals and to trigger a cellular response leading to the adaptation of the cells to their environment. Biochemical assays have been extensively used to reveal the interaction between membrane proteins. However, such analyses do not reveal the unique and complex composition of the membrane proteins of the different plant cell types. Here, we conducted a comprehensive analysis of the expression of Arabidopsis membrane proteins in the different cell types composing the root. Specifically, we analyzed the expression of genes encoding membrane proteins interacting in large complexes. We found that …

Cell-Type-Specific Profiling Of The Arabidopsis Thaliana Membrane Protein-Encoding Genes, Sergio Alan Cervantes-Pérez, Marc Libault

Department of Agronomy and Horticulture: Faculty Publications

Membrane proteins work in large complexes to perceive and transduce external signals and to trigger a cellular response leading to the adaptation of the cells to their environment. Biochemical assays have been extensively used to reveal the interaction between membrane proteins. However, such analyses do not reveal the unique and complex composition of the membrane proteins of the different plant cell types. Here, we conducted a comprehensive analysis of the expression of Arabidopsis membrane proteins in the different cell types composing the root. Specifically, we analyzed the expression of genes encoding membrane proteins interacting in large complexes. We found that …

Terror En El Orinoco: Formas Del Espanto Animal Y Vegetal En La Historia Natural De José Gumilla, Roberto E. Martinez Bachrich

Dissertations, Theses, and Capstone Projects

“Terror in the Orinoco: Forms of Animal and Plant Horror in the Natural History of José Gumilla”, analyzes the repertoire of terrifying plants and animals that the Spanish Jesuit missionary José Gumilla described in his 1745 compendium El Orinoco Ilustrado y Defendido —a work that, surprisingly, has not been read from the perspective of animal and plant studies. Based on Spanish old models of the natural history of the Indies (Fernández de Oviedo, Acosta), Gumilla reconstructs an archive of monstrous beings (alligators, piranhas, snakes, poisonous plants) that challenges old medieval religious discourses when confronted with the nascent illustrated discourses. My …

Herbaceous Production Lost To Tree Encroachment In United States Rangelands, Scott L. Morford, Brady W. Allred, Dirac L. Twidwell Jr, Matthew O. Jones, Jeremy D. Maestas, Caleb P. Roberts, David E. Naugle

Department of Agronomy and Horticulture: Faculty Publications

1. Rangelands of the United States provide ecosystem services that benefit society and rural economies. Native tree encroachment is often overlooked as a primary threat to rangelands due to the slow pace of tree cover expansion and the positive public perception of trees. Still, tree encroachment fragments these landscapes and reduces herbaceous production, thereby threatening habitat quality for grassland wildlife and the economic sustainability of animal agriculture.

2. Recent innovations in satellite remote sensing permit the tracking of tree encroachment and the corresponding impact on herbaceous production. We analysed tree cover change and herbaceous production across the western United States …

From Free-Energy Profiles To Activation Free Energies, Johannes C. Dietschreit, Dennis J. Diestler, Andreas Hulm, Christian Ochsenfeld, Rafael Gómez-Bombarelli

Department of Agronomy and Horticulture: Faculty Publications

Given a chemical reaction going from reactant (R) to the product (P) on a potential energy surface (PES) and a collective variable (CV) discriminating between R and P, we define the free-energy profile (FEP) as the logarithm of the marginal Boltzmann distribution of the CV. This FEP is not a true free energy. Nevertheless, it is common to treat the FEP as the “free-energy” analog of the minimum potential energy path and to take the activation free energy, ΔF‡ _RP, as the difference between the maximum at the transition state and the minimum at R. We show that this …

A Yield Comparison Between Small-Plot And On-Farm Foliar Fungicide Trials In Soybean And Maize, Anabelle Laurent, Emily Heaton, Peter Kyveryga, David Makowski, Laila A. Puntel, Alison E. Robertson, Laura J. Thompson, Fernando Miguez

Department of Agronomy and Horticulture: Faculty Publications

Agronomic research provides management recommendations based on small-plot trials (SPTs) and on-farm trials (OFTs) with very different characteristics. SPTs are traditionally conducted at agricultural experiment stations by research institutes or universities, while OFTs are conducted under commercial-scale conditions and managed by farmers using their own equipment. Several researchers claimed that discrepancies could occur between these two types of trials, which can make the extrapolation of results from SPTs to the farm level difficult. In our study, we conducted an extensive comparison of small-plot and on-farm trials to analyze the effect of foliar fungicide application on maize and soybean yields. We …

Snp Discovery In Proso Millet (Panicum Miliaceum L.) Using Lowpass Genome Sequencing, Rituraj Khound, Guangchao Sun, Ravi V. Mural, James C. Schnable, Dipak K. Santra

Department of Agronomy and Horticulture: Faculty Publications

Domesticated ~10,000 years ago in northern China, Proso millet (Panicum miliaceum L.) is a climate-resilient and human health-promoting cereal crop. The genome size of this self-pollinated allotetraploid is 923 Mb. Proso millet seeds are an important part of the human diet in many countries. In the USA, its use is restricted to the birdseed and pet food market. Proso millet is witnessing gradual demand in the global human health and wellness food market owing to its health-promoting properties such as low glycemic index and gluten-free. The breeding efforts for developing improved proso millet cultivars are hindered by the …

The Influence Of Water Stress And Rootstocks On Nutrient Uptake And Gas Exchange Of Young Peach Trees, Naif Alshammari

All Theses

Young peach trees are often rainfed in the southeastern U.S. and periods of dry weather can cause tree water deficit that can be detrimental to orchard productivity. There is a lack of understanding of the influence of water regimes and rootstocks on the nutritional status and gas exchange of the peach tree. Most peach growers in Southeastern United States do not irrigate their young peach trees because they do not produce fruit until the third leaf. In this region, peach growers use Guardian as a rootstock, although MP-29 is also interesting for growers with orchards that have Armillaria root rot …

The Genetic Basis For Panicle Trait Variation In Switchgrass (Panicum Virgatum), Li Zhang, Alice Macqueen, Xiaoyu Weng, Kathrine D. Behrman, Jason Bonnette, John L. Reilley, Francis M. Rouquette, Philip A. Fay, Yanqi Wu, Felix B. Fritschi, Robert B. Mitchell, David B. Lowry, Arvid R. Boe, Thomas E. Juenger

Department of Agronomy and Horticulture: Faculty Publications

Key message: We investigate the genetic basis of panicle architecture in switchgrass in two mapping populations across a latitudinal gradient, and find many stable, repeatable genetic effects and limited genetic interactions with the environment. Abstract: Grass species exhibit large diversity in panicle architecture influenced by genes, the environment, and their interaction. The genetic study of panicle architecture in perennial grasses is limited. In this study, we evaluate the genetic basis of panicle architecture including panicle length, primary branching number, and secondary branching number in an outcrossed switchgrass QTL population grown across ten field sites in the central USA through multi-environment …

Characterization And Selection Of Hop Cultivars Adapted To Nebraska, Kristina Alas

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Hop (Humulus lupulus L.) is an ingredient in the beer brewing industry that provides beer its flavor and aroma. High demand from the brewing industry has encouraged production outside of the traditional Pacific Northwest, the primary production region. Producers in the Midwest are attempting to grow cultivars adapted to the Pacific Northwest, but environmental differences have caused low yields and changes in secondary metabolite content. To aid producers, a regional breeding program was initiated to develop cultivars adapted to the Midwest. Success of any breeding program relies on the selection of genetically superior parents to generate progeny with the …

Mycorrhizae In Maize (Zea Mays L.) Cropping Systems Respond Differently To Nitrogen Fertilization Under Increasing Crop Rotational Diversity, Morgan R. Mcpherson

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Arbuscular mycorrhizal fungi (AMF) remain a vital obligate symbiont of nearly all plants. It is well established that the symbiosis between AMF and host plant improves plant nutrient acquisition, alleviates abiotic and biotic environmental stressors, defends against plant pathogens, and contributes to overall plant fitness and productivity through modification of the soil habitat. Modifications include increased soil aggregation and stability, carbon sequestration through provision of fungal wall precursors to soil organic matter (SOM) formation, and enhanced nutrient cycling in the mycorrhizosphere. The goal of this dissertation was to assess how AMF respond to nitrogen (N) fertilization regimes in maize cropping …

Chromosome Number Evolution, Phylogeography, And The Effects Of Climate Change On Species Distributions In Polyploid Plant Systems, Courtney H. Babin

LSU New Orleans Theses and Dissertations

Polyploidy, a term used to describe organisms with cells having more than two paired sets of chromosomes, is a significant driver of diversification among land plants. Over a century of research has advanced our understanding of polyploidization in some taxa, but polyploid organisms remain understudied. In this dissertation, I investigate chromosome number evolution, phylogeographic structure, genetic differentiation, and the effects of climate change on ploidy level distribution using polyploid plant systems. In the first chapter, I inferred a molecular phylogeny of Allium, an economically important genus that includes cultivated crops and ornamentals, to investigate evolutionary transitions in chromosome number …

Hiding In Plain Sight: Accounting For Rate Heterogeneity In Trait Evolution Models, James Boyko

Graduate Theses and Dissertations

Within the last four decades, phylogenetic comparative methods have become the defacto method of analysis for comparative biologists. The availability of high-quality comparative datasets has been matched by an explosion of possible phylogenetic models. In large part, the efforts to increase the realism of phylogenetic comparative methods has been successful as evidenced by their widespread use. To this extensive literature, my contributions are modest. I have focused my dissertation work on two main themes. First, most phenotypic evolution is not independent of other phenotypes. Changes in a particular character may influence changes in another and modeling these characters in isolation …

Water Sourcing Strategies Of Desert Vegetation In Varying Soil Textures With Vegetation Competition: A Stable Isotope Analysis, Martha Elizabeth Gardea

Open Access Theses & Dissertations

Recent studies have suggested an ecohydrological separation of water exists in the northern Chihuahuan Desert, where vegetation types, rainfall regimes, and location in the terrain seem to determine the type of water (e.g. isotopically depleted or enriched in 18O and 2H) plants are able to source and use throughout their growing cycle. That work found evidence of creosote (Larrea tridentata) and mesquite (Prosopis glandulosa) using tightly bound soil water at both a site on an ephemeral channel and a site in higher elevated flat area because the isotopic signature of plant water at the beginning of the study did not …

Flora Of Doe Mountain Recreation Area, Johnson County, Tennessee, Benjamin Mccullough

Electronic Theses and Dissertations

A botanical inventory of Doe Mountain Recreation Area (DMRA) in northeastern Tennessee was conducted to help guide conservation-based management. A total of 484 species were found in DMRA, comprising 94 families, and 285 genera, 10 species listed in the state rare plant list, and 76 exotic species. Two species, Liatris virgata and Lycopodiella inundata, were new state records. Water in the Lycopodiella seep was an order of magnitude more acid than at other sites. An analysis of the wildland-urban interface showed that only 13% of the area was classified as uninhabited. The inventory-invasion index, introduced to quantify the relative …