Botany Commons^™

Effects Of Cyclic Heat Stress On The Acute Inflammatory Response In Broilers, Alessandro J. Rocchi, Chrysta N. Beck, Jossie M. Santamaria, Gisela F. Erf

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Heat stress (HS) is a growing concern in broiler production. Little is known regarding the effect of HS on immune function. To examine the effects of HS on innate immunity, the local- and systemic-inflammatory responses to lipopolysaccharide (LPS) were examined in Cobb 500 male broiler chicks reared under thermoneutral (TN) or cyclic HS conditions. Beginning at four days of age, HS birds were subjected to 35 °C from 8:00 a.m. to 10:00 p.m. and TN temperatures from 10:00 p.m. to 8:00 a.m. At 37 days of age, four groups of broilers were formed: LPS-TN (8 broilers), phosphate-buffered saline (PBS)-TN (4 …

A New Annual Satureja (Lamiaceae) Species From Turkey With Molecular Evidence, And Lectotypification Of Two Species, Hayri̇ Duman, Tuncay Di̇rmenci̇, Taner Özcan

Turkish Journal of Botany

A new species, Satureja hasturkii, is described using morphological characters and molecular studies in this study. The new species is closely related to S. hortensis but differs from it by very lax thyrsoid inflorescence, longer pedicels, shorter calyx, upper and lower calyx teeth, and corolla. In order to support the morphological data, molecular studies are also done using nrITS and cptrnL-F gene regions. nrITS data gives better results in phylogenetic analysis. It is pointed out that the closest relatives of S. hasturkii are S. hortensis and S. laxiflora. The fact that having annual life forms of …

Drought Stress Effects On Morphophysiological And Quality Characteristics Of Commercial Carrot Cultivars, Muhammad Daniyal Junaid, Zahi̇de Nesli̇han Öztürk, Ali̇ Fuat Gökçe

Turkish Journal of Botany

The effects of drought stress on plant growth and development are getting more pronounced due to increasing influence of climate change on environmental stresses. Current study was devised to explore the drought effects on eight commercial carrot cultivars, having different root colors as a base for further studies to understand the response of carrot. Drought stress for 10 days was applied to carrot plants at taproot formation stage in semicontrolled greenhouse. The results revealed that orange and yellow colored carrot cultivars exhibited the least decline in physiological functioning (relative water contents and dry matter) that assisted in maintaining higher yield …

The Influence Of Cover Crops On Grassland Restoration, Josh C. Poland

Masters Theses

Grassland ecosystems comprise almost a fourth of all Earth’s land mass; however, due to ever-increasing rates of agricultural land conversion, these habitats are facing a large-scale biodiversity crisis. The degradation of grasslands extends beyond physical impacts, such as soil erosion, to include the loss of soil microbial communities as a serious risk to the health and productivity of these habitats. Cover cropping, commonly used in agriculture to revive agricultural soils, has rarely been applied to grassland restorations, and could combat or reverse the harmful effects of modern agriculture techniques. For the first chapter of my thesis, I used a greenhouse …

The Collaboration Between Art And Botany, Hee So, Sierra Beecher

UROP Posters

Using past research from my work study with Dr. Beecher where a team of students focused on quantitative and diagnostic anatomies of salt marsh and beach grasses on the Atlantic coasts, I used the research collected and created vector-based diagrams that were easily readable for Biology students at VCU. These illustrations were used in a manuscript we have been preparing, which has been accepted by the “Castanea” scientific journal. During the research fellowship, I was able to design a poster that portrayed the benefits of collaboration between art and botany, and Dr. Beecher presented the poster at the ASPB conference …

การเปลี่ยนแปลงโครงสร้างรากและการสร้างเซลล์บอร์เดอร์ของรากข้าวที่มีการแสดงออกของยีน Osnuc1-S สูงภายใต้ภาวะเครียดจากความเค็ม, จรรยา แปลกทิ้ง

Chulalongkorn University Theses and Dissertations (Chula ETD)

โครงสร้างรากของข้าวมีการเปลี่ยนแปลงสอดคล้องกับภาวะที่ไม่เหมาะสม นอกจากนี้ ปลายรากยังปกคลุมด้วยเซลล์บอร์เดอร์ของราก (root border cells; RBCs) ซึ่งมีการผลัดออกเป็นเซลล์เดี่ยวที่มีชีวิตและมีกิจกรรมการหลั่งสารที่สามารถปกป้องเนื้อเยื่อเจริญปลายรากได้ ข้าวมีการเจริญเติบโตและมีผลผลิตที่ลดลงในภาวะเค็ม จึงมีการศึกษาเกี่ยวกับยีนที่เกี่ยวข้องกับการทนเค็มในข้าว ซึ่งพบว่าข้าวจาปอนิกา (Oryza sativa L. ssp. japonica) ที่มีการแสดงออกของยีน OsNUC1-S สูง มีความทนเค็มมากกว่าข้าวพันธุ์ปกติ งานวิจัยนี้จึงศึกษาว่าการเปลี่ยนแปลงโครงสร้างรากและ RBCs เกี่ยวข้องกับความทนเค็มที่เกิดจากบทบาทของยีนนี้หรือไม่ โดยปลูกต้นกล้าข้าวพันธุ์ปกติและสายพันธุ์ที่มีการแสดงออกของยีน OsNUC1-S สูง 3 สายพันธุ์ ให้ได้รับภาวะเค็ม (85 mM NaCl) เป็นเวลา 12 วัน ผลการศึกษาพบว่า ภาวะเค็มไม่มีผลต่อการเติบโตของข้าวพันธุ์ปกติ แต่กลับมีผลลดน้ำหนักของส่วนเหนือดินในข้าวสายพันธุ์ที่ดัดแปรพันธุกรรม TOsL1 แสดงความอ่อนแอต่อภาวะเค็มมากที่สุด โดยมีการลดลงของน้ำหนักแห้งรากและจำนวนรากพิเศษ ภาวะเค็มมีผลเพิ่มความยาวรากปฐมภูมิอย่างชัดเจนในข้าวพันธุ์ปกติและ TOsL1 ส่วนจำนวนรากแขนงลดลงเนื่องจากภาวะเค็มในข้าวทุกสายพันธุ์ ผลดังกล่าวแสดงให้เห็นว่าการเปลี่ยนแปลงโครงสร้างรากในภาวะเค็มไม่มีความสัมพันธ์กับบทบาทของยีน OsNUC1-S โดยตรง สำหรับการผลิต RBCs ในภาวะปกติ พบว่ามีรูปแบบที่คล้ายคลึงกันในข้าวทั้งสองกลุ่ม โดยมีการผลิตจำนวน RBCs สูงสุด 3000-4000 เซลล์ ตั้งแต่เมื่อรากอายุยังน้อยที่มีความยาวราก 0.5-1.0 เซนติเมตร จำแนกชนิด RBCs ตามอัตราส่วนความยาวต่อความกว้างของเซลล์ได้ 3 ชนิด โดยมีขนาดของเซลล์ที่เล็กและมีความหนาของชั้นสารเมือกที่บางกว่าข้าวอินดิกา เมื่อย้ายต้นกล้าที่มีความยาวราก 1.0±0.2 เซนติเมตร ให้ได้รับภาวะเค็มเป็นเวลา 24 ชั่วโมง พบว่าข้าวพันธุ์ปกติมีร้อยละของต้นกล้าที่รากหยุดการเจริญเติบโตมากกว่าสายพันธุ์ที่ดัดแปรพันธุกรรม และมีจำนวน RBCs ที่ลดลง นอกจากนี้ ต้นกล้าที่สามารถเจริญเติบโตและมีความยาวรากเพิ่มขึ้นเป็น 1.5±0.2 และ 2.0±0.2 เซนติเมตร มีจำนวน RBCs ที่ลดลงในข้าวพันธุ์ปกติแต่ไม่เปลี่ยนแปลงในสายพันธุ์ที่ดัดแปรพันธุกรรม ดังนั้น จึงสรุปได้ว่า ข้าวจาปอนิกามีการเปลี่ยนแปลงโครงสร้างรากในภาวะเค็มโดยเพิ่มความยาวรากปฐมภูมิซึ่งไม่เกี่ยวข้องโดยตรงกับยีน OsNUC1-S และมีความสัมพันธ์ระหว่าง RBCs กับความสามารถในการเจริญเติบโตของรากในภาวะเค็ม ซึ่งสอดคล้องกับการมียีน OsNUC1-S ซึ่งเป็นยีนทนเค็มในข้าว

Changes And Relationships Of Soil Aluminum, Organic Matter, And Hydrogen Ion Concentration With Rye Cover Crop, Arron Wilder

Cal Poly Humboldt theses and projects

Soil acidity is a common agricultural problem worldwide, as approximately 50% of all potentially arable soils are affected by pH limitations. At soil pH 3+) is considered to be the chemical form of aluminum in acid soil that hinders plant growth the most. Potentially, soil organic matter (SOM) can ameliorate the toxic effects of Al³⁺ on plants and microbes by binding with Al³⁺, thus preventing Al³⁺ (and other species of aluminum) from interacting in the rhizosphere. Increasing SOM also increases soil health indicators (i.e., microbial activity, soil water holding capacity, aggregate stability, porosity, etc.) while the …

Forage Grass Growth Habit And Allelopathy In Birdsfoot Trefoil Germination And Establishment, Nathan El Blake

Graduate Theses, Dissertations, and Problem Reports (ETD)

Birdsfoot trefoil (Lotus corniculatus L.) (BFT) is a high quality, tanniniferous forage legume that has been shown to mitigate gastrointestinal nematode (GIN) infection in sheep. Reliable establishment of birdsfoot trefoil in existing pastures is difficult due to BFT’s poor seedling vigor, susceptibility to crown and root rot, and, perhaps chiefly, competition from other pasture plants. Most existing pastures in the Northeastern United States are primarily composed of cool-season grasses, many of which can be highly competitive. A common form of interplant competition is allelopathy, which occurs when secondary metabolites produced by a plant inhibit a necessary growth process of …

Winter Dynamics Of Storm Water Management Ponds And Winter Tolerance In Three Aquatic Plant Species, Patrick Strzalkowski

Theses and Dissertations (Comprehensive)

The vast majority of the research into the performance of stormwater management ponds (SWMPs) has been performed in warm regions or during the warmer seasons in temperate regions. It is presumed that SWMPs are inactive in the winter as any potential stormwater is trapped in snow and ice. The main goal of this thesis was to test this presumption and to study the dynamics and performance of three SWMPs during the winter. Remote water level loggers were installed into the three SWMPs and daily grab samples from the influents and effluents were taken and analyzed for total phosphorus (TP), chloride, …

A Cladoxylopsid With Complex Vascular Architecture From The Early Devonian Battery Point Formation (Québec, Canada), Jessica Chu

Cal Poly Humboldt theses and projects

Cladoxylopsids are seed-free plants that formed the world’s earliest forests and gave rise to horsetails (sphenopsids), but their evolutionary origins are poorly understood. Here, I describe a new Early Devonian cladoxylopsid from the Emsian (400-395 Ma) Battery Point Formation (Québec, Canada). The structural features of this plant indicate taxonomic affinity to cladoxylalean cladoxylopsids and its phylogenetic position supports placement in genus Cladoxylon (the proposed name, Cladoxylon kespekianum sp. nov., to be formalized by peer-reviewed publication). Specimens of this plant are permineralized in calcium carbonate and were studied using the acetate peel technique with light and electron microscopy. Cladoxylon kespekianum is …

Assessing The Recovery Of Forest Understory Vegetation After Clearcut Logging Across A 445-Year Chronosequence, Molly Smith Metok

Cal Poly Humboldt theses and projects

The conversion of natural forested lands to managed forests has reduced the amount of older, structurally diverse forests worldwide. In conifer forests of the Pacific Northwest (USA) – where the understory plant communities comprise only 1% of forest biomass but represent 90% of the plant species richness – the long-term impacts of timber harvesting are not fully understood. I used a chronosequence of forests in southwestern Oregon that ranged from 25 to 445 years of age to compare changes in plant communities in logged (i.e., managed) stands with that of stands in late succession and old growth conditions. The chronosequence …

Macrolichen Inventory Of The Horse Mountain Botanical Area, Six Rivers National Forest, California, Usa, Sarah Norvell Conway

Cal Poly Humboldt theses and projects

Macrolichen diversity and community composition was determined for an area of high botanical interest in the Coast Ranges of Northern California – the Horse Mountain Botanical Area (HMBA) in Six Rivers National Forest. The Coast Ranges have been suggested to have high epiphytic macrolichen diversity, yet detailed site-specific macrolichen surveys are lacking for the area. Here we present comprehensive data on macrolichens of the HMBA integrated with environmental metadata at the landscape level. Twenty 0.4 ha sampling plots were positioned across the varying habitats of the HMBA and macrolichens were intensively sampled from all substrata. Out of 888 total collections, …

Transformation And Gene Editing In The Bioenergy Grass Miscanthus, Anthony Trieu, Mohammad B. Belaff, Pradeepa Hirannaiah, Shilpa Manjunatha, Rebekah Wood, Yokshitha Bathula, Rebecca L. Billingsley, Anjali Arpan, Erik J. Sacks, Thomas E. Clemente, Stephen P. Moose, Nancy A. Reichert, Kankshita Swaminathan

Department of Agronomy and Horticulture: Faculty Publications

Background: Miscanthus, a C4 member of Poaceae, is a promising perennial crop for bioenergy, renewable bioproducts, and carbon sequestration. Species of interest include nothospecies M. x giganteus and its parental species M. sacchariforus and M. sinensis. Use of biotechnology-based procedures to genetically improve Miscanthus, to date, have only included plant transformation procedures for introduction of exogenous genes into the host genome at random, non-targeted sites.

Results: We developed gene editing procedures for Miscanthus using CRISPR/Cas9 that enabled the mutation of a specific (targeted) endogenous gene to knock out its function. Classified as paleo-allopolyploids (duplicated ancient …

Yield Prediction Through Integration Of Genetic, Environment, And Management Data Through Deep Learning, Daniel R. Kick, Jason G. Wallace, James C. Schnable, Judith M. Kolkman, Barış Alaca, Timothy M. Beissinger, Jode Edwards, David Ertl, Sherry Flint-Garcia, Joseph L. Gage, Candice N. Hirsch, Joseph E. Knoll, Natalia De Leon, Dayane C. Lima, Danilo E. Moreta, Maninder P. Singh, Addie Thompson, Teclemariam Weldekidan, Jacob D. Washburn

Department of Agronomy and Horticulture: Faculty Publications

Accurate prediction of the phenotypic outcomes produced by different combinations of genotypes, environments, and management interventions remains a key goal in biology with direct applications to agriculture, research, and conservation. The past decades have seen an expansion of new methods applied toward this goal. Here we predict maize yield using deep neural networks, compare the efficacy of 2 model development methods, and contextualize model performance using conventional linear and machine learning models. We examine the usefulness of incorporating interactions between disparate data types. We find deep learning and best linear unbiased predictor (BLUP) models with interactions had the best overall …

Establishment Of The Invasive Cactus Moth, Cactoblastis Cactorum (Berg) (Lepidoptera: Pyralidae) In Pakistan: A Potential Threat To Cultivated, Ornamental And Wild Opuntia Spp. (Cactaceae), Muhammad Ather Rafi, Harry Pavulaan, Muhammad Islam, Muhammad Ashfaq, Haseeb Kamran, Walija Fayaz, Gul Naz Parveen, Riffat Sultana, Ahmad Zia, Waqar Ahmed, Qudrat Ullah, Muhammad Qasim, Falak Naz, Nazeer Ahmed, Muhammad Tariq Khan, Muhammad Saeed, Jalal Hayat Khan

The Taxonomic Report of the International Lepidoptera Survey

Subsequent to the significant accomplishment of biological control of Opuntia weeds in Australia, the larvae of the cactus moth, Cactoblastis cactorum (native to parts of South America), were released in many countries for the biological control of native Opuntia species (Simmonds and Bennett, 1966). Inauspiciously, larvae were also released in the Caribbean, where the moth spread naturally and by the human support all over the region (García-Turudi et al., 1971). Its enhanced dissemination rate and the biological potential for invasiveness, suggests that the cactus moth is likely to become an invasive pest of Opuntia in the Southeast United States, Mexico, …

Genetic Variation In Common Bunt Resistance In Synthetic Hexaploid Wheat, Amira M. I. Mourad, Alexey Morgounov, P. Stephen Baenziger, Samar M. Esmail

Department of Agronomy and Horticulture: Faculty Publications

Common bunt (caused by Tilletia caries and T. Foetida) is a major wheat disease. It occurs frequently in the USA and Turkey and damages grain yield and quality. Seed treatment with fungicides is an effective method to control this disease. However, using fungicides in organic and low-income fields is forbidden, and planting resistant cultivars are preferred. Due to the highly effective use of fungicides, little effort has been put into breeding resistant genotypes. In addition, the genetic diversity for this trait is low in modern wheat germplasm. Synthetic wheat genotypes were reported as an effective source to increase the …

Inheritance Of 2,4‑Dichlorophenoxyacetic Acid (2,4‑D) Resistance In Amaranthus Palmeri, Chandrima Shyam, Dallas E. Peterson, Amit J. Jhala, Mithila Jugulam

Department of Agronomy and Horticulture: Faculty Publications

In this study, the inheritance of 2,4-D resistance in a multiple herbicide-resistant Palmer amaranth (KCTR) was investigated. Direct and reciprocal crosses were performed using 2,4-D-resistant KCTR and susceptible KSS plants to generate F₁ progenies. 2,4-D dose–response assays were conducted to evaluate the response of progenies from each F₁ family along with KCTR and KSS plants in controlled environmental growth chambers. Additionally, 2,4-D-resistant male and female plants from each of the F₁ families were used in pairwise crosses to generate pseudo-F₂ families. Segregation (resistance or susceptibility) of progenies from the F₂ families in response to a …

Bio Prospecting Of Lapachol Producing Endophytic Fungi, Chandrashekar Srinivasa, Sushma Pradeep, Shashank M Patil, Ramith Ramu, Shiva Prasad Kollur, Shashanka K Prasad, Chandan Shivamallu

International Journal of Health and Allied Sciences

Background: The association of endophytic fungi with medicinal plants has been one of the evolving areas of research in the past few decades. The secondary metabolites produced owing to such associations have been recognised for a wide range of biological activities. Objectives: The aim of the present review is to highlight the isolation of lapachol from endophytic microorganisms with an emphasis on its biotransformation to improve its efficacy. Methodology: The researchers followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) criteria. Published scientific articles on endophytes, host-pathogen interaction and lapachol isolation were collected from reputed journals from …

A Stealth Health Approach To Dietary Fibre, P. Stephen Baenziger, Katherine Anna Frels, Steve Greenspan, Julie Jones, Alison Lovegrove, Devin J. Rose, Peter Shewry, Rod Wallace

Department of Agronomy and Horticulture: Faculty Publications

Average dietary fibre intakes have increased little in the past twenty years in many countries, including the USA¹ . Multi-million-dollar campaigns promoting fruits, vegetables, whole grains and other foods high in fibre have delivered only small changes in diets² , and consumers have not changed from traditional staples to whole-grain options³ . UK millers report that consumption of whole-wheat bread has actually declined over the past decade (P. Shewry, personal communication). In the US, white flour, which is lower in fibre than whole-wheat flour, accounts for nearly 40% of the fibre intake⁴ . We believe that …

Genetic Variations Of Narcissus Tazetta And Selected Narcissus Cultivars Based On The Sequence Analysis Of Nrits And TrnL‑Is‑TrnF Regions, Seo Young Park, Mi Jin Jeon, Young Hee Joung, Keenan L. Amundsen, Gianluca Burchi, Angelo Porcelli, Mark S. Roh

Department of Agronomy and Horticulture: Faculty Publications

Narcissus tazetta var. chinensis (N. taz. var. chi.) accessions collected from the coastal areas of China, Korea, and Japan were compared with accessions of lower ranks of Narcissus tazetta (N. taz.) from Italy to study the genetic variations and with several morphologically similar cultivars available in the trade. Sequences of the internal transcribed spacer (ITS) regions of nuclear ribosomal DNA (nrITS) dendrogram and the chloroplast trnL-IS-trnF intergeneric spacer-gene (cpIS) were analyzed by maximum likelihood (ML) and posterior probability using STRUCTURE program. All single fower form of N. taz. var. chi. collected from China, Korea, and …