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10,753 full-text articles. Page 136 of 217.

Ultrastructural Observations In Somatic Embryogenesis Of Natural Tetraploid Trifolium Pratense L., HATİCE ÇÖLGEÇEN, HATİCE NURHAN BÜYÜKKARTAL, UFUK KOCA ÇALIŞKAN 2016 TÜBİTAK

Ultrastructural Observations In Somatic Embryogenesis Of Natural Tetraploid Trifolium Pratense L., Hati̇ce Çölgeçen, Hati̇ce Nurhan Büyükkartal, Ufuk Koca Çalişkan

Turkish Journal of Botany

Previous reports of plant regeneration of natural tetraploid T. pratense L. `Elçi' could be realized only through the apical meristem calli. In order to proceed to the production stage, other regeneration methods need to be tried. Aseptic seedlings were used for the production of somatic embryos through various 2,4-D and kinetin trials. Nonuniform external callus cells with translucent cytoplasm were observed in various developmental stages of somatic embryos. Beneath these cells, there were uniformly aligned, dark-stained embryo cells with dense cytoplasm. Despite the similar developmental stages and cell characteristics of zygotic and somatic embryos, the walls of somatic embryo cells …


Genetic Relationships Among The Genera Cicer L., Lathyrus L., Lens Mill., And Vicia L., Together With Similarity Of Lens Taxa Based On Morphological And Aflp Markers, ZEYNEP YILDIZDOĞAN, CENGİZ İKTEN, NEDİM MUTLU, CENGİZ TOKER 2016 TÜBİTAK

Genetic Relationships Among The Genera Cicer L., Lathyrus L., Lens Mill., And Vicia L., Together With Similarity Of Lens Taxa Based On Morphological And Aflp Markers, Zeynep Yildizdoğan, Cengi̇z İkten, Nedi̇m Mutlu, Cengi̇z Toker

Turkish Journal of Botany

The molecular characterization of the seven taxa in the genus Lens Mill. with Vicia montbretii has not been performed, and species of the genera Vicia L., Lathyrus L., and Cicer L. using AFLP markers have not been elucidated so far. The objectives of the present study were the assessment of (i) genetic relationships among the genera Lens, Vicia, Lathyrus, and Cicer based on AFLP markers, (ii) relationships within the genus Lens including the accessions of L. lamottei Czefr., L. tomentosus Ladiz., and L. odemensis Ladiz., based on morphological and AFLP data, and (iii) position of the species Vicia montbretii (Fisch. …


Comparison Of Rdna Regions (Its, Lsu, And Ssu) Of Some Aspergillus, Penicillium, And Talaromyces Spp., RASİME DEMİREL 2016 TÜBİTAK

Comparison Of Rdna Regions (Its, Lsu, And Ssu) Of Some Aspergillus, Penicillium, And Talaromyces Spp., Rasi̇me Demi̇rel

Turkish Journal of Botany

Aspergillus, Penicillium, and Talaromyces spp. are commonly found worldwide and include industrially and medicinally important members. These genera are deeply related with life from soil to health hazards via their industrial products, surface proteins, biodiversity, food spoilage, mycotoxins, etc. These three genera progressively encounter important taxonomic revisions, such as the one fungus-one name concept, and various methods are available to establish their evolutionary and phylogenetic relationships. Besides morphological, microscopic, and extrolite analyses, nucleotide sequence analysis is the most preferred method. In particular, rDNA regions, such as the internal transcribed spacer (ITS), large subunit (LSU), and small subunit (SSU), are commonly …


Positive Interactions In Temperate And Tropical Alpine Plant Communities: The Role Of Facilitation In Species-Specific Interactions, Invasion, And Landscape-Scale Distribution, Nicole Hupp 2016 University of Montana, Missoula

Positive Interactions In Temperate And Tropical Alpine Plant Communities: The Role Of Facilitation In Species-Specific Interactions, Invasion, And Landscape-Scale Distribution, Nicole Hupp

Graduate Student Theses, Dissertations, & Professional Papers

No abstract provided.


Pollinator Sharing Between Mimulus Ringens And Coflowering Plant Species In Northeastern Ohio, Andrew M. Wuellner 2016 The University of Akron

Pollinator Sharing Between Mimulus Ringens And Coflowering Plant Species In Northeastern Ohio, Andrew M. Wuellner

Williams Honors College, Honors Research Projects

Competition between plants for pollinators can have serious impacts on plant reproduction; these impacts depend on many factors, such as plant abundance, plant diversity, floral abundance, pollinator abundance, and pollinator preference. The way pollinators move among and between coflowering species can tell us more about how these factors affect competition. In this study, we examine the movement patterns of flower visitors to Mimulus ringens and coflowering species in Northeastern Ohio through several types of observations. In addition, we measured the density and diversity of floral units with 20-30 meter transects across each study site. There were six total study sites, …


Combating A Global Threat To A Clonal Crop: Banana Black Sigatoka Pathogen Pseudocercospora Fijiensis (Synonym Mycosphaerella Fijiensis) Genomes Reveal Clues For Disease Control, Rafael E. Arango-Isaza, Caucasella Diaz-Trujillo, Braham Deep Singh Dhillon, Andrea L. Aerts, Jean Carlier, Charles F. Crane, Tristan V. De Jong, Ineke M. De Vries, Robert A. Dietrich, Andrew D. Farmer, Claudia Fortes Fereira, Suzana A L Garcia, Mauricio Guzmán, Richard C. Hamelin, Erika A. Lindquist, Rahim Mehrabi, Olman Quiros, Jeremy Schmultz, Harris J. Shapiro, Elizabeth Reynolds, Gabriel Scalliet, Manoel Teixeira Souza, Ioannis Stergiopoulos, Theo A J Van Der Lee, Pierre J G M De Wit, Marie Françoise Zapater, Lute Harm Zwiers, Igor V. Grigoriev, Stephen B. Goodwin, Gert Hj J Kema 2016 Corporacion para Investigaciones Biologicas, Plant Biotechnology Unit, Medellin, Colombia

Combating A Global Threat To A Clonal Crop: Banana Black Sigatoka Pathogen Pseudocercospora Fijiensis (Synonym Mycosphaerella Fijiensis) Genomes Reveal Clues For Disease Control, Rafael E. Arango-Isaza, Caucasella Diaz-Trujillo, Braham Deep Singh Dhillon, Andrea L. Aerts, Jean Carlier, Charles F. Crane, Tristan V. De Jong, Ineke M. De Vries, Robert A. Dietrich, Andrew D. Farmer, Claudia Fortes Fereira, Suzana A L Garcia, Mauricio Guzmán, Richard C. Hamelin, Erika A. Lindquist, Rahim Mehrabi, Olman Quiros, Jeremy Schmultz, Harris J. Shapiro, Elizabeth Reynolds, Gabriel Scalliet, Manoel Teixeira Souza, Ioannis Stergiopoulos, Theo A J Van Der Lee, Pierre J G M De Wit, Marie Françoise Zapater, Lute Harm Zwiers, Igor V. Grigoriev, Stephen B. Goodwin, Gert Hj J Kema

Department of Botany and Plant Pathology Faculty Publications

Black Sigatoka or black leaf streak disease, caused by the Dothideomycete fungus Pseudocercospora fijiensis (previously: Mycosphaerella fijiensis), is the most significant foliar disease of banana worldwide. Due to the lack of effective host resistance, management of this disease requires frequent fungicide applications, which greatly increase the economic and environmental costs to produce banana. Weekly applications in most banana plantations lead to rapid evolution of fungicide-resistant strains within populations causing disease-control failures throughout the world. Given its extremely high economic importance, two strains of P. fijiensis were sequenced and assembled with the aid of a new genetic linkage map. The 74-Mb …


Proteomic Profiling Of Maize Opaque Endosperm Mutants Reveals Selective Accumulation Of Lysine-Enriched Proteins, Kyla J. Morton, Shangang Jia, Chi Zhang, David R. Holding 2016 University of Nebraska-Lincoln

Proteomic Profiling Of Maize Opaque Endosperm Mutants Reveals Selective Accumulation Of Lysine-Enriched Proteins, Kyla J. Morton, Shangang Jia, Chi Zhang, David R. Holding

Department of Agronomy and Horticulture: Faculty Publications

Reduced prolamin (zein) accumulation and defective endoplasmic reticulum (ER) body formation occurs in maize opaque endosperm mutants opaque2 (o2), floury2 (fl2), defective endosperm*B30 (DeB30), and Mucronate (Mc), whereas other opaque mutants such as opaque1 (o1) and floury1 (fl1) are normal in these regards. This suggests that other factors contribute to kernel texture. A liquid chromatography approach coupled with tandem mass spectrometry (LC-MS/MS) proteomics was used to compare non-zein proteins of nearly isogenic opaque endosperm mutants. In total, 2762 proteins were identified that were enriched for biological processes such …


Shrubland Resilience Varies Across Soil Types: Implications For Operationalizing Resilience In Ecological Restoration, Carissa L. Wonkka, Dirac L. Twidwell, Jacob B. West, William E. Rogers 2016 Texas A&M University

Shrubland Resilience Varies Across Soil Types: Implications For Operationalizing Resilience In Ecological Restoration, Carissa L. Wonkka, Dirac L. Twidwell, Jacob B. West, William E. Rogers

Department of Agronomy and Horticulture: Faculty Publications

In ecosystems with alternative stable states, restoration success can be thought of as overcoming the resilience of an undesirable state to promote an alternative state that yields greater ecosystem services. Since greater resilience of undesirable states translates into reduced restoration potential, quantifying differences in resilience can enhance restoration planning. In the context of shrub- encroached rangeland restoration, shrubland resilience is the capacity of a woody vegetated state to absorb management interventions designed to produce a more desirable grass- dominated state, and remain within its current regime. Therefore, differences in the resilience of a state can be quantifi ed in a …


Growing Sensitivity Of Maize To Water Scarcity Under Climate Change, Qingfeng Meng, Xinping Chen, David B. Lobell, Zhenling Cui, Yi Zhang, Haishun Yang, Fusuo Zhang 2016 China Agricultural University

Growing Sensitivity Of Maize To Water Scarcity Under Climate Change, Qingfeng Meng, Xinping Chen, David B. Lobell, Zhenling Cui, Yi Zhang, Haishun Yang, Fusuo Zhang

Department of Agronomy and Horticulture: Faculty Publications

Climate change can reduce crop yields and thereby threaten food security. The current measures used to adapt to climate change involve avoiding crops yield decrease, however, the limitations of such measures due to water and other resources scarcity have not been well understood. Here, we quantify how the sensitivity of maize to water availability has increased because of the shift toward longer-maturing varieties during last three decades in the Chinese Maize Belt (CMB). We report that modern, longer-maturing varieties have extended the growing period by an average of 8 days and have significantly offset the negative impacts of climate change …


Information Thermodynamics Of Cytosine Dna Methylation, Robersy Sanchez Rodriguez, Sally Ann Mackenzie 2016 University of Nebraska-Lincoln

Information Thermodynamics Of Cytosine Dna Methylation, Robersy Sanchez Rodriguez, Sally Ann Mackenzie

Department of Agronomy and Horticulture: Faculty Publications

Cytosine DNA methylation (CDM) is a stable epigenetic modification to the genome and a widespread regulatory process in living organisms that involves multicomponent molecular machines. Genome-wide cytosine methylation patterning participates in the epigenetic reprogramming of a cell, suggesting that the biological information contained within methylation positions may be amenable to decoding. Adaptation to a new cellular or organismal environment also implies the potential for genome-wide redistribution of CDM changes that will ensure the stability of DNA molecules. This raises the question of whether or not we would be able to sort out the regulatory methylation signals from the CDM background …


Horizontal Gene Acquisitions, Mobile Element Proliferation, And Genome Decay In The Host-Restricted Plant Pathogen Erwinia Tracheiphila, Lori R. Shapiro, Erin D. Scully, Timonthy J. Straub, Jihye Park, Andrew G. Stephenson, Gwyn A. Beattie, Mark L. Gleason, Roberto Kolter, Miguel C. Coelho, Consuelo M. De Moraes, Mark C. Mescher, Olga Zhaxybayeva 2016 Harvard University

Horizontal Gene Acquisitions, Mobile Element Proliferation, And Genome Decay In The Host-Restricted Plant Pathogen Erwinia Tracheiphila, Lori R. Shapiro, Erin D. Scully, Timonthy J. Straub, Jihye Park, Andrew G. Stephenson, Gwyn A. Beattie, Mark L. Gleason, Roberto Kolter, Miguel C. Coelho, Consuelo M. De Moraes, Mark C. Mescher, Olga Zhaxybayeva

Department of Agronomy and Horticulture: Faculty Publications

Modern industrial agriculture depends on high-density cultivation of genetically similar crop plants, creating favorable conditions for the emergence of novel pathogens with increased fitness in managed compared with ecologically intact settings. Here, we present the genome sequence of six strains of the cucurbit bacterial wilt pathogen Erwinia tracheiphila (Enterobacteriaceae) isolated from infected squash plants in New York, Pennsylvania, Kentucky, and Michigan. These genomes exhibit a high proportion of recent horizontal gene acquisitions, invasion and remarkable amplification of mobile genetic elements, and pseudogenization of approximately 20% of the coding sequences. These genome attributes indicate that E. tracheiphila recently emerged as a …


Herbicide Options For Effective Weed Management In Dry Directseeded Rice Under Scented Rice-Wheat Rotation Of Western Indo- Gangetic Plains, Vijay Singh, Mangi L. Jat, Zahoor A. Ganie, Bhagirath S. Chauhan, Raj K. Gupta 2016 University of Arkansas

Herbicide Options For Effective Weed Management In Dry Directseeded Rice Under Scented Rice-Wheat Rotation Of Western Indo- Gangetic Plains, Vijay Singh, Mangi L. Jat, Zahoor A. Ganie, Bhagirath S. Chauhan, Raj K. Gupta

Department of Agronomy and Horticulture: Faculty Publications

Farmers' participatory field trials were conducted at Madhuban, and Taraori, the two participatory experimental sites/locations of the Cereal Systems Initiative for South Asia (CSISA), a collaborative project of IRRI and CIMMYT in Karnal district of Haryana, India, during Kharif (wet season) 2010 and 2011. This research aimed to evaluate preemergence (PRE) and postemergence (POST) herbicides for providing feasible and economically viable weed management options to farmers for predominant scented rice varieties. Treatments with pendimethalin PRE fb bispyribac-sodium þ azimsulfuron POST had lower weed biomass at 45 days after sowing (DAS). At Madhuban, highest grain yield of scented basmati rice (3.43 …


Prospects For Increasing Sugarcane And Bioethanol Production On Existing Crop Area In Brazil, Fabio R. Martin, Geraldo B. Martha,JR, Kenneth G. Cassman, Patricio Grassini 2016 University of São Paulo

Prospects For Increasing Sugarcane And Bioethanol Production On Existing Crop Area In Brazil, Fabio R. Martin, Geraldo B. Martha,Jr, Kenneth G. Cassman, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

This article assesses sugarcane yield gaps (YG) in Brazil to determine the degree to which production can be increased without land expansion. In our scenario assessments, we evaluated how much of the projected sugarcane demand to 2024 (for both sugar and bioethanol) can be satisfied through YG closure. The current national average yield is 62% of yield potential estimated for rainfed conditions (i.e., a YG of 38%). Continuing the historical rate of yield gain is not sufficient to meet the projected demand without an area expansion by 5% and 45% for lowand high-demand scenarios, respectively. Closing the exploitable YG to …


Glyphosate-Resistant Weed Control And Soybean Injury In Response To Different Ppo-Inhibiting Herbicides, Jatinder S. Aulakh, P. S. Chahal, Amit J. Jhala 2016 Connecticut Agricultural Experiment Station, New Haven

Glyphosate-Resistant Weed Control And Soybean Injury In Response To Different Ppo-Inhibiting Herbicides, Jatinder S. Aulakh, P. S. Chahal, Amit J. Jhala

Department of Agronomy and Horticulture: Faculty Publications

In Nebraska, 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) as well as acetolactate synthase (ALS)-inhibitor-resistant weeds occur in many soybean fields where herbicides from these modes-of-action have been frequently used in the past. Currently, the protoporphyrinogen oxidase (PPO)-inhibitors are the only effective herbicides for POST control of both glyphosate- and ALS-inhibitor-resistant weeds in soybean. Greenhouse experiments were conducted in 2014 to evaluate the efficacy of PPO-inhibitors applied POST for the control of three glyphosate-resistant (GR) weeds and potential for soybean injury, when applied at two growth stages. All herbicide treatments controlled 10- and 20-cm tall GR common waterhemp ≥ 95% at 21 DAT. …


Estimation Of The Degree Of Diversity For Some Iraqi Wheat Varieties Through Issr, Srap And Rapd Markers, Dhafir H. Al-Kaab, Majid Sh. Hamdalla, Ismail M. Dweikat, Noora J. Al-Saedi 2016 University of Baghdad

Estimation Of The Degree Of Diversity For Some Iraqi Wheat Varieties Through Issr, Srap And Rapd Markers, Dhafir H. Al-Kaab, Majid Sh. Hamdalla, Ismail M. Dweikat, Noora J. Al-Saedi

Department of Agronomy and Horticulture: Faculty Publications

DNA-based molecular markers such as Inter Simple Sequence Repeat (ISSR), Sequence-Related Amplified polymorphism (SRAP) and Random Amplified Polymorphic DNA (RAPD) were used in this study to examine the genetic differences among sixteen Iraqi wheat varieties. Seventy three primers out of 177 were reproducible and showed clear amplified bands. The degree of genetic diversity, Polymorphism information content (PIC) and resolving power (RP) were estimated. All the studied molecular markers were informative and showed good ability to classify and distinguish 16 wheat varieties. Total number of polymorphic bands is 134, 221 and 55 for ISSR, SRAP and RAPD respectively. PIC and RP …


Nutrients In The Nexus, Eric A. Davidson, Rachel L. Nifong, Richard B. Ferguson, Cheryl Palm, Deanna L. Osmond, Jill S. Baron 2016 University of Maryland Center for Environmental Science, Frostburg

Nutrients In The Nexus, Eric A. Davidson, Rachel L. Nifong, Richard B. Ferguson, Cheryl Palm, Deanna L. Osmond, Jill S. Baron

Department of Agronomy and Horticulture: Faculty Publications

Synthetic nitrogen (N) fertilizer has enabled modern agriculture to greatly improve human nutrition during the twentieth century, but it has also created unintended human health and environmental pollution challenges for the twentyfirst century. Averaged globally, about half of the fertilizer-N applied to farms is removed with the crops, while the other half remains in the soil or is lost from farmers’ fields, resulting in water and air pollution. As human population continues to grow and food security improves in the developing world, the dual development goals of producing more nutritious food with low pollution will require both technological and socioeconomic …


The C-Terminal Motif Of Siago1b Is Required For The Regulation Of Growth, Development And Stress Responses In Foxtail Millet (Setaria Italica (L.) P. Beauv), Xiaotong Liu, Sha Tang, Guanqing Jia, James C. Schnable, Haixia Su, Chanjuan Tang, Hui Zhi, Xianmin Diao 2016 Chinese Academy of Agricultural Sciences

The C-Terminal Motif Of Siago1b Is Required For The Regulation Of Growth, Development And Stress Responses In Foxtail Millet (Setaria Italica (L.) P. Beauv), Xiaotong Liu, Sha Tang, Guanqing Jia, James C. Schnable, Haixia Su, Chanjuan Tang, Hui Zhi, Xianmin Diao

Department of Agronomy and Horticulture: Faculty Publications

Foxtail millet (Setaria italica (L.) P. Beauv), which belongs to the Panicoideae tribe of the Poaceae, is an important grain crop widely grown in Northern China and India. It is currently developing into a novel model species for functional genomics of the Panicoideae as a result of its fully available reference genome sequence, small diploid genome (2n=18, ~510 Mb), short life cycle, small stature and prolific seed production. Argonaute 1 (AGO1), belonging to the argonaute (AGO) protein family, recruits small RNAs and regulates plant growth and development. Here, we characterized an AGO1 mutant (siago1b) in foxtail millet, which …


Genomic Prediction Models For Grain Yield Of Spring Bread Wheat In Diverse Agro-Ecological Zones, C. Saint Pierre, Juan Burgueño, Jose Crossa, G. Fuentes Dávila, P. Figueroa López, E. Solís Moya, J. Ireta Moreno, V. M. Hernández Muela, V. M. Zamora Villa, P. Vikram, K. Mathews, C. Sansaloni, D. Sehgal, Diego Jarquin, P. Wenzl, Sukhwinder Singh 2016 International Maize and Wheat Improvement Center

Genomic Prediction Models For Grain Yield Of Spring Bread Wheat In Diverse Agro-Ecological Zones, C. Saint Pierre, Juan Burgueño, Jose Crossa, G. Fuentes Dávila, P. Figueroa López, E. Solís Moya, J. Ireta Moreno, V. M. Hernández Muela, V. M. Zamora Villa, P. Vikram, K. Mathews, C. Sansaloni, D. Sehgal, Diego Jarquin, P. Wenzl, Sukhwinder Singh

Department of Agronomy and Horticulture: Faculty Publications

Genomic and pedigree predictions for grain yield and agronomic traits were carried out using high density molecular data on a set of 803 spring wheat lines that were evaluated in 5 sites characterized by several environmental co-variables. Seven statistical models were tested using two random cross-validations schemes. Two other prediction problems were studied, namely predicting the lines’ performance at one site with another (pairwise-site) and at untested sites (leave-one-site-out). Grain yield ranged from 3.7 to 9.0 t ha−1 across sites. The best predictability was observed when genotypic and pedigree data were included in the models and their interaction with …


Genomic Prediction Of Gene Bank Wheat Landraces, José Crossa, Diego Jarquin, Jorge Franco, Paulino Pérez-Rodríguez, Juan Burgueño, Carolina Saint-Pierre, Prashant Vikram, Carolina Sansaloni, Cesar Petroli, Denis Akdemir, Clay Sneller, Matthew Reynolds, Maria Tattaris, Thomas Payne, Carlos Guzman, Roberto J. Peña, Peter Wenzl, Sukhwinder Singh 2016 International Maize and Wheat improvement Center (CIMMYT)

Genomic Prediction Of Gene Bank Wheat Landraces, José Crossa, Diego Jarquin, Jorge Franco, Paulino Pérez-Rodríguez, Juan Burgueño, Carolina Saint-Pierre, Prashant Vikram, Carolina Sansaloni, Cesar Petroli, Denis Akdemir, Clay Sneller, Matthew Reynolds, Maria Tattaris, Thomas Payne, Carlos Guzman, Roberto J. Peña, Peter Wenzl, Sukhwinder Singh

Department of Agronomy and Horticulture: Faculty Publications

This study examines genomic prediction within 8416 Mexican landrace accessions and 2403 Iranian landrace accessions stored in gene banks. The Mexican and Iranian collections were evaluated in separate field trials, including an optimum environment for several traits, and in two separate environments (drought, D and heat, H) for the highly heritable traits, days to heading (DTH), and days to maturity (DTM). Analyses accounting and not accounting for population structure were performed. Genomic prediction models include genotype × environment interaction (G × E). Two alternative prediction strategies were studied: (1) random cross-validation of the data in 20% training (TRN) and 80% …


Registration Of ‘Ne05548’ (Husker Genetics Brand Panhandle) Hard Red Winter Wheat, P. Stephen Baenziger, R. A. Graybosch, T. Regassa, R. N. Klein, Greg R. Kruger, D. K. Santra, L. Xu, D. J. Rose, S. N. Wegulo, Y. Jin, J. Kolmer, G. L. Hein, M.-S. Chen, G. Bai, R. L. Bowden, J. Poland 2016 University of Nebraska-Lincoln

Registration Of ‘Ne05548’ (Husker Genetics Brand Panhandle) Hard Red Winter Wheat, P. Stephen Baenziger, R. A. Graybosch, T. Regassa, R. N. Klein, Greg R. Kruger, D. K. Santra, L. Xu, D. J. Rose, S. N. Wegulo, Y. Jin, J. Kolmer, G. L. Hein, M.-S. Chen, G. Bai, R. L. Bowden, J. Poland

Department of Agronomy and Horticulture: Faculty Publications

Western Nebraska wheat producers and those in adjacent areas want taller wheat (Triticum aestivum L.) cultivars that retain their height under drought for better harvestability. ‘NE05548’ (Reg. No. CV-1117, PI 670462) hard red winter wheat was developed cooperatively by the Nebraska Agricultural Experiment Station and the USDA-ARS and released in January 2014 by the developing institutions. NE05548 was released primarily for its superior performance under rainfed conditions in western Nebraska and adjacent areas of the Great Plains and its tall plant stature. NE05548 was selected from the cross NE97426/NE98574 made in 1999 where the pedigree of NE97426 is ‘Brigantina’/2*‘Arapahoe’ …


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