Agricultural Science Commons^™

Genes Identified By Visible Mutant Phenotypes Show Increased Bias Toward One Of Two Subgenomes Of Maize, James C. Schnable, Michael Freeling

Department of Agronomy and Horticulture: Faculty Publications

Not all genes are created equal. Despite being supported by sequence conservation and expression data, knockout homozygotes of many genes show no visible effects, at least under laboratory conditions. We have identified a set of maize (Zea mays L.) genes which have been the subject of a disproportionate share of publications recorded at MaizeGDB. We manually anchored these ‘‘classical’’ maize genes to gene models in the B73 reference genome, and identified syntenic orthologs in other grass genomes. In addition to proofing the most recent version 2 maize gene models, we show that a subset of these genes, those that …

Dose–Sensitivity, Conserved Non-Coding Sequences, And Duplicate Gene Retention Through Multiple Tetraploidies In The Grasses, James C. Schnable, Brent S. Pedersen, Sabarinath Subramaniam, Michael Freeling

Department of Agronomy and Horticulture: Faculty Publications

Whole genome duplications, or tetraploidies, are an important source of increased gene content. Following whole genome duplication, duplicate copies of many genes are lost from the genome. This loss of genes is biased both in the classes of genes deleted and the subgenome from which they are lost. Many or all classes are genes preferentially retained as duplicate copies are engaged in dose sensitive protein–protein interactions, such that deletion of any one duplicate upsets the status quo of subunit concentrations, and presumably lowers fitness as a result. Transcription factors are also preferentially retained following every whole genome duplications studied. This …

Inta Segovia, A New Sorghum Variety For The Dry Zones Of Nicaragua, Intsormil

INTSORMIL Impacts and Bulletins

The Sandanista Government through the Instituto Nicaraguense de Tecnologia (INTA) officially released the new improved sorghum variety, INTA Segovia on January 14, 2011. This improved variety will benefit the resource-poor farmers that grow sorghum on the hillsides (see photo) in dry zones (40 percent of the land area in Nicaragua). This variety will have a significant economic impact in Nicaragua and in neighboring countries as it produces well in drought prone areas which lack sufficient rainfall for growing maize. INTA Segovia, under drought conditions, produces higher yields than currently grown sorghum varieties. Drought tolerance is due to the large root …

Proceedings Of The 38th Annual Meeting, Southern Soybean Disease Workers (March 9-10, 2011, Pensacola Beach, Florida), Boyd Padgett, Tom Allen, Jason Bond, Cliff Coker, Stephen R. Koenning

Southern Soybean Disease Workers: Conference Proceedings

Contents

Southern United States Soybean Disease Loss Estimates for 2010. Compiled by SR Koenning

Poster presentation

Soybean Varieties and Breeding Lines Shown With Resistance to Reniform Nematode in Greenhouse Tests, 1998-2010 R. T. Robbins, and L. E. Jackson

Oral presentations

Frogeye Leaf Spot (Cercorosa sojina) symposium (Boyd Padgett, moderator)

Development of Baseline Cercospora sojina Sensitivity Levels to Quinone Outside Inhibitor Fungicides and Monitoring for Resistance. GR Zhang and CA Bradley

Soybean Pathogen Found to be Resistant to Fungicides MA Newman and CA Bradley

Strobilurin-resistant Cercospora sojina in Kentucky: Field History. D Hershman

Graduate student paper competition (Tom Allen, …

Comparison Of Various Configurations Of Cdc-Type Traps For The Collection Of Phlebotomus Papatasi Scopoli In Southern Israel, Daniel L. Kline, Jerome Hogsette, Gunter Muller

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We conducted two experiments to determine the best CDC-trap configuration for catching male and female Phlebotomus papatasi. First, visual features were evaluated. Standard CDC traps were modified to have black or white catch bags, black or white lids, or no lids and these were tried in different combinations. Significantly more male sand flies were caught by darker traps; significantly more females were captured by traps with either all black or a combination of black and white features. Attraction may be due to dark color or contrast in colors. CDC traps with suction and the following features were also evaluated: …

Wayne E. Sabbe Arkansas Soil Fertility Studies 2010, Nathan A. Slaton

Arkansas Agricultural Experiment Station Research Series

Rapid technological changes in crop management and production require that the research efforts be presented in an expeditious manner. The contributions of soil fertility and fertilizers are major production factors in all Arkansas crops.

The Fundamentals Of Nutrient Management, Rhonda Miller, Clark Israelsen, Jim Bowcutt, Neils Hansen

All Current Publications

This publication gives best management practices of nutrient management in livestock and dairy facilities.

Instalan Molino Para Producir Harina De Sorgo: Con Este Molino Se Prevé Procesar, Al Mes, Alrededor De 48 Quintales De Harina, Berta Nely Menjívar

INTSORMIL Impacts and Bulletins

Panificadores de la Asociación Cooperativa de Cuscatlán (ACPACUS de R. L.), aglutinados en la Mesa Nacional de Panificadores de El Salvador (MENAPAES), en compañía de autoridades del Centro Nacional de Tecnología Agropecuaria y Forestal Enrique Álvarez Córdova (CENTA) y del Instituto para la Investigación del Sorgo y Mijo (INTSORMIL), inauguraron la puesta en marcha del primer molino de martillo, con el cual estarán procesando harina de sorgo (maicillo) para utilizarla en la industria de la panificación.

La adquisición de este molino es uno de los primeros esfuerzos de la MENAPAES en la búsqueda de otras materias primas para la producción …

Sorghum And Millet Research Program Receives Grant, Daily Nebraskan

INTSORMIL Impacts and Bulletins

The International Sorghum and Millet Collaborative Research Support Program (INTSORMIL) at the University of Nebraska-Lincoln recently received a $1.1 million grant to work with Central American countries to increase sorghum foliage.

A Rain Garden Grows In Bridgeport, Michael Dietz

Wrack Lines

Rain gardens can be used to help communities adapt to climate change. A warmer New England means more precipitation, which means more polluted runoff water--unless a rain garden, planted in a depression, can sequester the excess water and use it to grow attractive plants.

Managing Complexity In Modern Farming, Ross Kingwell

Natural Resources Research Articles

Modern farming in Australia is no longer simple. Farms are large, multi-enterprise businesses underpinned by expensive capital investments, changing production technologies, volatile markets and social challenges. The complexity of modern broadacre farming leads to the question: what is the nature of the relationship between farm business complexity and farm profitability? This study uses bioeconomic farm modelling and employs eight measures of complexity to examine the profitability and complexity of a wide range of broadacre farming systems in Australia. Rank order correlations between farm profitability and each measure of complexity show inconsistent relationships, although the most profitable farming systems are found …

Intsormil Information, Daily Nebraskan

INTSORMIL Impacts and Bulletins

The International Sorghum and Millet Collaborative Research Support Program (INTSORMIL) at the University of Nebraska-Lincoln recently received at $1.1 million grant to work with Central American countries to increase sorghum foliage.

Report Of Nine Sorghum Flour Workshops, Report For Intsormil; Subject: “Utilization Of Cti Grinders For Small Scale Sorghum Flour Production In Central America”, Intsormil

INTSORMIL Scientific Publications

In Nicaragua, Sorghum grain has been a crops using only for animal feed. Fortunately Scientifics have been researched about its nutritional sorghum grain properties and some of the country that they have been interested to bring the technology about sorghum flour has been Nicaragua because its growths in 3 big areas; in the north, south and west region. Now, that is knew their characteristics and others, so INTA and INTSORMIL are diffusing the technology about sorghum flour to increase the production of sorghum grain, reduce the cost production of bread and some ethnics drinks, to improve the food security and …

Switchgrass, Kenneth P. Vogel, Gautam Sarath, Aaron J. Saathoff, Robert B. Mitchell

Department of Agronomy and Horticulture: Faculty Publications

17.1 Introduction

Switchgrass (Panicum virgatum L.) is a warm-season perennial grass that is native to North America that is being developed into a biomass energy crop. It has been used in pastures and for conservation purposes in the Great Plains and the Midwest, USA, for over 70 years.1 The research supporting its use as a pasture and conservation species was largely conducted by US Department of Agriculture (USDA) research programs, most notably the Agricultural Research Service (ARS) project located at the University of Nebraska-Lincoln and USDA Plant Materials Centers that are located throughout the United States. In this report, …

Identification Of A Second Asian Soybean Rust Resistance Gene In Hyuuga Soybean, Mandy D. Kendrick, Donna K. Harris, Bo-Keun Ha, D. L. Hyten, P. B. Cregan, Reid D. Frederick, H. Roger Boerma, Kerry F. Pedley

Department of Agronomy and Horticulture: Faculty Publications

Asian soybean rust (ASR) is an economically significant disease caused by the fungus Phakopsora pachyrhizi. The soybean genes Rpp3 and Rpp?(Hyuuga) confer resistance to specific isolates of the pathogen. Both genes map to chromosome 6 (Gm06) (linkage group [LG] C2). We recently identified 12 additional soybean accessions that harbor ASR resistance mapping to Gm06, within 5 centimorgans of Rpp3 and Rpp?(Hyuuga). To further characterize genotypes with resistance on Gm06, we used a set of eight P. pachyrhizi isolates collected from geographically diverse areas to inoculate plants and evaluate them for differential phenotypic responses. …

Mutational Analysis Of The Major Soybean Uref Paralogue Involved In Urease Activation, Joe C. Polacco, D. L. Hyten, Mônica Medeiros-Silva, David A. Sleper, Kristin D. Bilyeu

Department of Agronomy and Horticulture: Faculty Publications

The soybean genome duplicated ~14 and 45 million years ago and has many paralogous genes, including those in urease activation (emplacement of Ni and CO₂ in the active site). Activation requires the UreD and UreF proteins, each encoded by two paralogues. UreG, a third essential activation protein, is encoded by the single-copy Eu3, and eu3 mutants lack activity of both urease isozymes. eu2 has the same urease-negative phenotype, consistent with Eu2 being a single-copy gene, possibly encoding a Ni carrier. Unexpectedly, two eu2 alleles co-segregated with missense mutations in the chromosome 2 UreF paralogue (Ch02UreF), suggesting …

Cytokinin-Mediated Source ⁄Sink Modifications Improve Drought Tolerance And Increase Grain Yield In Rice Under Water-Stress, Zvi Peleg, Maria Reguera, Ellen Tumimbang, Harkamal Walia, Eduardo Blumwald

Department of Agronomy and Horticulture: Faculty Publications

Drought is the major environmental factor limiting crop productivity worldwide. We hypothesized that it is possible to enhance drought tolerance by delaying stress-induced senescence through the stress-induced synthesis of cytokinins in crop-plants. We generated transgenic rice (Oryza sativa) plants expressing an isopentenyltransferase (IPT) gene driven by PSARK, a stress- and maturation-induced promoter. Plants were tested for drought tolerance at two yield-sensitive developmental stages: pre- and post-anthesis. Under both treatments, the transgenic rice plants exhibited delayed response to stress with significantly higher grain yield (GY) when compared to wild-type plants. Gene expression analysis revealed a significant shift in expression of hormone-associated …

Evaluation Of Hha And Hha Sepb Mutant Strains Of Escherichia Coli O157:H7 As Bacterins For Reducing E. Coli O157:H7 Shedding In Cattle, Vijay K. Sharma, Evelyn A. Dean-Nystrom, Thomas A. Casey

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Escherichia coli O157:H7 colonizes cattle intestines by using the locus of enterocyte effacement (LEE)- encoded proteins. The induction of systemic immune response against LEE-encoded proteins, therefore, will prove effective in reducing E. coli O157:H7 colonization in cattle. The previous studies have demonstrated that a hha (encodes for a hemolysin expression modulating protein) deletion enhances expression of LEE-encoded proteins and a sepB (encodes an ATPase required for the secretion of LEE-encoded proteins) deletion results in intracellular accumulation of LEE proteins. In this study, we demonstrate the efficacy of the hha and hha sepB deletion mutants as bacterins for …

The Ability Of Selected Pupal Parasitoids (Hymenoptera: Pteromalidae) To Locate Stable Fly Hosts In A Soiled Equine Bedding Substrate, Jimmy B. Pitzer, Phillip E. Kaufman, Christopher Geden, Jerome A. Hogsette

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The ability of Spalangia cameroni Perkins, Spalangia endius Walker, and Muscidifurax raptorellus Kogan and Legner to locate and attack stable fly hosts was evaluated under laboratory conditions. Postfeeding third-instar stable fly larvae were released and allowed to pupate in two arena types: large 4.8 liter chambers containing a field-collected, soiled equine bedding substrate; or 120-ml plastic cups containing wood chips. At the time of fly pupariation, parasitoids were released and permitted 72 h to locate and attack hosts. On average, parasitism rates of freely accessible stable fly pupae in cups were not significantly different between parasitoid species. However, parasitism rates …

New Records For The Horse Fly Fauna (Diptera: Tabanidae) Of Jordan With Remarks On Ecology And Zoogeography, Günter Müller, Jerome Hogsette, Edita Revay, Vasiliy Kravchenko, Andrey Leshvanov, Yosef Schlein

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The horse fly fauna (Diptera: Tabanidae) of Jordan is, after Israel, the richest in the Levant, with 24 known species. During the 20-year project “The Ecology and Zoogeography of the Lepidoptera of the Near East,” we regularly collected blood-feeding flies, resulting in 11 additional species of Tabanidae for Jordan. The new records are: Atylotus quadrifarius (Loew, 1874), Chrysops caecutiens (Linnaeus, 1758), Dasyrhamphis nigritus (Fabricius, 1794), Haematopota pallens Loew, 1871, Nemorius irritans (Ricardo, 1901), Philipomyia graeca (Fabricius, 1794), Tabanus cordiger Meigen, 1820, Tabanus taeniola Palisot de Beauvois, 1806, Tabanus quatuornotatus Meigen, 1820, Tabanus separatus Effllatoun, 1930, and Tabanus spectabilis Loew, 1858. …