Physiology Commons^™

Hemodynamics At Maximum Exercise And Exercise Recovery In Freshman Football Recruits At A Bcs School, Krystal Doughty, Steven Martin, Brad Lambert, Jon Oliver, David Wier, John Tanguay, Justin Dobson, Katie Mccammon, S.F Crouse Facsm, J.S. Green Facsm

International Journal of Exercise Science: Conference Proceedings

To determine if blood pressures assessed during max exercise and exercise recovery differ in Freshman football player recruits classified according to body mass index categories (BMICAT). A group of 107 freshman football recruits (mean age=18.2yrs, mean height=187.2 cm, mean weight = 103.0 kg, mean BMI=27.4 kg/meters squared, mean percent fat= 18.3%.) underwent graded exercise testing on a treadmill. Height and weight were assessed and BMI was calculated as weight (kg) / height (m) squared. All 97 subjects were classified as either “normal weight”(NW) (N=38), “overweight” (OW)(N=41) or “obese” (OB)(N=28) according to the National Institutes of Health guidelines. Body fat percentage …

Active V. Passive Recovery: The Most Effective Level Relative To Swimming Performance, Kassie Krusely, Scott Mclean, Jimmy Smith

International Journal of Exercise Science: Conference Proceedings

To examine the effects of active recovery at two levels of intensity, as compared to passive recovery, on subsequent performance. Four male (height, 174.4 ± 8.1 cm; body mass, 73.5 ± 5.6 kg) and five female (height, 164.1 ± 7.9 cm; body mass, 65.0 ± 4.9 kg) members of a collegiate swim team participated in this study. All participants completed three randomly assigned experimental sessions that consisted of a 100-yd maximal-effort swim (TT1) followed by a 10 min recovery period at a specified intensity, and a subsequent 100-yard maximal effort swim (TT2). The PAS recovery condition involved passively resting on …

The Effects Of An Obesogenic Diet On Liver Oxysterol Metabolism In C57bl/6j Mice, Joshua S. Wooten, Huaizhu Wu, Joe L. Raya, Amir Mansoori, Xiao-Yuan Dai Perrard, Jerry Perrard, Ron C. Hoogeveen

International Journal of Exercise Science: Conference Proceedings

Oxysterols are key regulators of lipid metabolism and play a role in the etiology of atherosclerosis; however, our current understanding of tissue levels of oxysterols during different disease states such as obesity is limited. The purpose of this study was to quantify the effects of obesity induced by a high fat-cholesterol (HFC) diet on liver oxysterol metabolism. Male C57BL/6J mice were fed either a standard control diet (5.0% w/w fat, 0.03% w/w chol) or a HFC (21.0% w/w fat, 0.15% w/w chol) diet for 24 weeks. Comparisons between dietary groups were made with independent sample t-tests. Total body mass and …

Predictors Of Employee Adherence To Worksite Weight Loss Exercise And Nutrition Program, Brittany Crim, John Bartholomew

International Journal of Exercise Science: Conference Proceedings

Background: Worksite wellness programs (WWP) are becoming a popular means of addressing the obesity epidemic due to their convenience and potential benefit to insurance costs. Unfortunately, few have been evaluated. GET FIT (GF) is a theory-based, worksite exercise and nutrition program that is designed to prevent and treat obesity. Purpose: Adherence is the primary predictor of success to a weight loss program. This study was designed to identify predictors of: adherence to GF; and weight loss in conjunction with GF. Methods: Data was collected for 175 participants (n = 133 female). The mean age was 44.21 yrs. (+/-12.04). Body weight …

The Effect Of Short And Long Recovery Periods On The Contribution Of Oxidative Processes To Energy Expenditure During Multiple Bouts Of Supramaximal Exercise, Eric C. Olson, Kenneth V. Christensen, Adam Jajtner, Jen Copeland, Matt Unthank, Joel B. Mitchell

International Journal of Exercise Science: Conference Proceedings

The Effect of Short and Long Recovery Periods on the Contribution of Oxidative Processes to Energy Expenditure During Multiple Bouts of Supramaximal Exercise

Olson, E. (undergraduate), Christensen, K., Jajtner, A., Copeland, J., Unthank, M., and Mitchell, J. Exercise Physiology Lab, Texas Christian University, Ft. Worth, TX.

The contribution of oxidative energy production to multiple sprint exercises is of interest due to implications for the training needs of people engaging in anaerobic activities. PURPOSE: The purpose of this study was to examine the effect of short and long active recovery durations on oxidative and anaerobic contributions to energy output during maximal …

Effects Of Multiple Bouts Of Long-Duration Hindlimb Unloading And Recovery On Rat Plantaris Muscle, Emily E. Jaroszewski, Kevin L. Shimkus, Yasaman Shirazi-Fard, Harry A. Hogan, James D. Fluckey

International Journal of Exercise Science: Conference Proceedings

Exposure to microgravity results in a rapid reduction of muscle mass. However, few studies exist designed to examine the effects of multiple long-term exposures to microgravity with alternating recovery periods on skeletal muscle. To determine what happens to the recovery of skeletal muscle when faced with subsequent unloading and recovery periods. Male Sprague-Dawley (6 mo) were assigned to the following groups as shown in figure 1 below: 28d hindlimb unloading (1HU), 28d HU session followed by a 56d recovery bout of normal cage ambulation at 1g (1HU+REC), 2 cycles of 28d HU with a 56d recovery period between unloadings (2HU), …

Artificial Sweetener Sensing In The Human Mouth And Effect On Exercise Performance, James Mock

International Journal of Exercise Science: Conference Proceedings

Many studies have suggested that oral rinsing of a sweet carbohydrate can positively affect exercise performance by stimulating reward centers in the brain. However, few studies have been performed which compared the effects of a sweet carbohydrate with a sweet artificial sweetener. The purpose of this study was to examine the effects of a sweet carbohydrate rinse, an artificial sweetener rinse, and a water rinse on exercise performance. Eight college students completed a ten-minute trial ride and were able to perform significantly more work when rinsing with the carbohydrate or artificial sweetener rinses (Total work= 116.63 (20.3) kJ p=0.003, 117.04 …

Physical, Cardiovascular, & Metabolic Effects Of Non-Exercise Weighted Vest Training, Mattie A. Mccain, Scott P. Mclean Phd, Jimmy C. Smith Phd

International Journal of Exercise Science: Conference Proceedings

Introduction: The high prevalence of obesity and sedentary lifestyle contribute to the growing burden of health care costs, incidence of disease, and mortality, making a lifestyle that includes regular physical activity increasingly important. Low intensity resistance training has been studied as a possible intervention to increase physical activity in inactive individuals. Purpose: To determine the effects of a weighted vest treatment (WV) on steady state VO2, O2 deficit, VO2max, body mass index (BMI), and resting blood pressure (RBP). Methods: Fourteen college age women participated in a 4 week trial and completed both pre and posttest evaluations. These evaluations included a …

Effects Of Aging On Endothelium-Dependent Vasodilation In Cerebral Arteries, Amanda De Jong, Landon Evans, Elisa Boyd, Scott A. Spier

International Journal of Exercise Science: Conference Proceedings

Regional cerebral blood flow (CBF) decreases with aging. This reduction may be due to a diminished endothelium-dependent vasodilation in large cerebral arteries with aging in the absence of disease. The posterior communicating arteries (PCoA) supply blood flow to the midbrain areas where age-related regional decreases in blood flow have been observed. Furthermore, the PCoA appear to be clinically relevant in age-related cerebrovascular disorders. However, age-related changes in the vasoreactivity of PCoA have not been investigated in the absence of disease. The purpose of this study was to determine the effect of aging on endothelium-dependent vasodilation of PCoA in rats. Posterior …

Vitamin D Status Relative To Diet, Lifestyle, Injury, And Illness In College Athletes, Tanya M. Halliday, Nikki J. Peterson, Joi J. Thomas, Kent Kleppinger, Bruce W. Hollis, D. Enette Larson-Meyer

International Journal of Exercise Science: Conference Proceedings

Vitamin D deficiency is endemic in the general population; however, there is much to be learned about the vitamin D status of athletes. Purpose: The purposes of this study were to assess the prevalence of vitamin D insufficiency in collegiate athletes and to determine whether 25(OH)D concentrations are related to vitamin D intake, sun exposure, body composition, and risk for illness or athletic injury. Methods: 25(OH) vitamin D concentrations were measured in 41 athletes (18 men/23 women, 12 indoor/29 outdoor athletes) throughout the academic year. Dietary intake and lifestyle habits were assessed via questionnaire, bone density was measured by dual …

Ros-Mediated Localization Of Caveolin-3 In The Sarcolemma During Short-Term Mechanical Unloading, Jeff M. Hord, Mary Kunst, Kumar Joshi, Yang Lee, Daniel A. Martinez, John M. Lawler

International Journal of Exercise Science: Conference Proceedings

The dystroglycan complex (DGC) is a latticework of cell membrane proteins that provide a mechanical link to the cytoskeleton and initiate cell signaling that provides integrity, regulates protein turnover, stimulates growth, etc. Caveolin-3 has been identified as a muscle-specific isoform of the caveolin family of proteins, and in skeletal muscle, caveolin-3 is localized in caveolae within the sarcolemma. Evidence from Duchenne and Limb-Girdle Muscular Dystrophies suggests the involvement of caveolin-3 and reactive oxygen species (ROS) in the disruption of the DGC and thus myopathy. We examined ROS as upstream activators of caveolin-3 sarcolemmal localization in skeletal muscle during disuse atrophy. …

Effect Of Text Messaging And Cellphone Use On A Multi-Sensory Tracking Task, John-Paul Connell, Carla Brown, Chelsey Hannah, Shane Huff, Dr. Scott Marzilli

International Journal of Exercise Science: Conference Proceedings

This experiment studied subjects’ performance on a continuous multisensory tracking task. Different theories suggest that there is a decrease in performance due to attentional capacity; furthermore, talking or texting on a mobile phone will negatively affect task performance. Twenty-four college-age students were recruited to be tested on the Biodex Balance System SD with the texting condition, talking on a mobile phone condition, and control condition. Results showed that text messaging while performing a continuous multisensory tracking task negatively affected performance. There was no significant difference, however, between the control condition and talking on a mobile phone while completing the task. …

Reciprocal Regulation Of Trek-1 Channels By Arachidonic Acid And Crh In Mouse Corticotropes, Andy K. Lee, James L. Smart, Marcelo Rubinstein, Malcolm J. Low, Amy Tse

Faculty Publications - Department of Biological & Molecular Science

Arachidonic acid (AA) is generated in the anterior pituitary gland upon stimulation by the ACTH secretagogue, CRH. Using the patch clamp technique,we examined the action of AA on the excitability of single pituitary corticotropes obtained from a transgenic mouse strain that expresses the enhanced green fluorescent protein driven by the proopiomelanocortin promoter. CRH evoked depolarization, but AA caused hyperpolarization. Under voltage clamp condition, AA caused a rapid inhibition of the delayed rectifier K current and then increased a background K current. Inhibition of AA metabolism did not prevent the activation of the K current by AA, suggesting a direct action …

Aberrant Promoter Cpg Methylation Is A Mechanism For Impaired Phd3 Expression In A Diverse Set Of Malignant Cells., Trenton L. Place, Matthew P. Fitzgerald, Sujatha Venkataraman, Sabine U. Vorrink, Adam J. Case, Melissa L.T. Teoh, Frederick E. Domann

Journal Articles: Cellular & Integrative Physiology

BACKGROUND: The prolyl-hydroxylase domain family of enzymes (PHD1-3) plays an important role in the cellular response to hypoxia by negatively regulating HIF-α proteins. Disruption of this process can lead to up-regulation of factors that promote tumorigenesis. We observed decreased basal expression of PHD3 in prostate cancer tissue and tumor cell lines representing diverse tissues of origin. Furthermore, some cancer lines displayed a failure of PHD3 mRNA induction when introduced to a hypoxic environment. This study explores the mechanism by which malignancies neither basally express PHD3 nor induce PHD3 under hypoxic conditions.

METHODOLOGY/PRINCIPAL FINDINGS: Using bisulfite sequencing and methylated DNA enrichment …

Human Chondrosarcoma Cells Acquire An Epithelial-Like Gene Expression Pattern Via An Epigenetic Switch: Evidence For Mesenchymal-Epithelial Transition During Sarcomagenesis., Matthew P. Fitzgerald, Francoise Gourronc, Melissa L.T. Teoh, Matthew J. Provenzano, Adam J. Case, James A. Martin, Frederick E. Domann

Journal Articles: Cellular & Integrative Physiology

Chondrocytes are mesenchymally derived cells that reportedly acquire some epithelial characteristics; however, whether this is a progression through a mesenchymal to epithelial transition (MET) during chondrosarcoma development is still a matter of investigation. We observed that chondrosarcoma cells acquired the expression of four epithelial markers, E-cadherin,desmocollin 3, maspin, and 14-3-3σ, all of which are governed epigenetically through cytosine methylation. Indeed, loss of cytosine methylation was tightly associated with acquired expression of both maspin and 14-3-3σ in chondrosarcomas. In contrast, chondrocyte cells were negative for maspin and 14-3-3σ and displayed nearly complete DNA methylation. Robust activation of these genes was also …

Genetic And Maternal Effects On Valproic Acid Teratogenesis In C57bl/6j And Dba/2j Mice, Chris Downing, Jami Biers, Colin Larson, Alexi Kimball, Hali Wright, Takamasa Ishii, David Gilliam, Thomas Johnson

SPS Faculty Publications

Valproic acid (VPA) is used worldwide to treat epilepsy, migraine headaches and bipolar disorder. However, VPA is teratogenic and in utero exposure can lead to congenital malformations. Using inbred C57BL/6J (B6) and DBA/2J (D2) mice, we asked whether genetic variation could play a role in susceptibility to VPA teratogenesis. While B6 fetuses were more susceptible than D2 fetuses to digit and vertebral malformations, D2 fetuses were more susceptible to rib malformations. In a reciprocal cross between B6 and D2, genetically identical F1 mice carried in a B6 mother had a greater percentage of vertebral malformations following prenatal VPA exposure than …

Altered Regulation Of Mitogen-Activated Protein Kinases And Glut4 Expression In Skeletal Muscle, Liver, Heart And Kidney Of Obese Zucker Rat, Sudarsanam Kundla

Theses, Dissertations and Capstones

Metabolic syndrome is characterized by insulin resistance and hyperglycemia. The molecular mechanism(s) underlying this alteration in skeletal muscle, liver, kidneys and heart in metabolic syndrome patients are presently unclear. Recent data have demonstrated that the p38and extracellular-signal-regulated kinases (ERK 1/2) - mitogen-activated protein kinase (MAPK) proteins may be involved in the regulation of (Glucose transporter type 4) GLUT4 expression levels. Other data have suggested that miRNA may also play a role. Purpose: The purpose of this study is to compare the tissue content, phosphorylation of protein kinases (AKT), p38, c-JUN Nterminal kinase(JNK), ERK1/2, GLUT4 and transcriptional factor Myocyte enhancer factor-2 …

Effect Of Dimethylarginine Dimethylaminohydrolase In The Development Of Salt Sensitivity, Samar Abdulla Nasser

Wayne State University Dissertations

Salt sensitivity is associated with a rise in blood pressure (BP) occurring during sodium loading and/or a fall in BP during sodium restriction that exceeds random fluctuations in BP. Salt sensitivity is more common in African American than Caucasian hypertensives and is also present, in normotensive African Americans. The mechanism or mechanisms resulting in salt-sensitive hypertension are multiple and include both activation of the renin angiotensin system via increases in angiotensin II and reductions in the endogenous vasodilator, nitric oxide (NO). An important means of NO downregulation is through asymmetric dimethylarginine (ADMA), an endogenous NO inhibitor, which is largely metabolized …

Hemorrhagic Stroke, Kelsey Bigham

Natural Sciences Student Research Presentations

No abstract provided.

Characterization Of The Expression Of Bdnf And Cgrp And Their Regulatory Pathways In Dorsal Root Ganglion During Cystitis., Sharon Yu

Theses and Dissertations

Interstitial cystitis is a chronic debilitating disease that causes pain and increased frequency of micturition, amongst other symptoms, without any identifiable cause. This disease affects a large number of the population, yet the etiology is still unknown. The present study aimed to characterize BDNF and CGRP—two neuropeptides that have both been proven to play an important role in the transmission of pain as well as in hypersensitivity. The signaling pathways regulating the expression of the two neuropeptides were also examined. Results revealed that BDNF protein expression levels increased in both L1 and L6 DRG following 48 hours post CYP-induced cystitis. …