Molecular Pathways Involved In Calcineurin Inhibitor Nephrotoxicity In Kidney Allograft Transplants,
2011
Virginia Commonwealth University
Molecular Pathways Involved In Calcineurin Inhibitor Nephrotoxicity In Kidney Allograft Transplants, Huong Nguyen
Theses and Dissertations
ABSTRACT MOLECULAR MECHANISMS AND GENE SIGNATURES INVOVLED IN CALCINEURIN INHIBITOR NEPHROTOXICITY IN KIDNEY ALLOGRAFT By Huong Le Diem Nguyen, M.S. A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Physiology at Virginia Commonwealth University. Virginia Commonwealth University, 2011. Major Director: Valeria Mas, Ph.D. Associate Professor, Department of Surgery and Pathology Director of Molecular Transplant Research Laboratory, Division of Transplant Calcineurin inhibitors (CNI), cyclosporin A and tacrolimus, are potent immunosuppressive agents but induce toxicities causing damages and graft dysfunction, and have been suggested to contribute to late-term loss of graft in kidney transplant …
Mechanisms Of Prenatal High-Salt "Fetal Programming" Resulting In Stress Hyperresponsiveness In The Adult Female Offspring In The Sprague Dawley Rat.,
2011
Brigham Young University - Provo
Mechanisms Of Prenatal High-Salt "Fetal Programming" Resulting In Stress Hyperresponsiveness In The Adult Female Offspring In The Sprague Dawley Rat., Clinton L. Johnson
Theses and Dissertations
Female offspring of Sprague-Dawley rats fed a high-salt diet (HS) during pregnancy show an enhancement of mean arterial pressure (MAP) and heart rate (HR) response to acute stress in adulthood compared to offspring whose mothers were fed a normal-salt diet (NS) [1]. In the present study, we first examined the expression of soluble epoxide hydrolase (SEH) protein in brain tissue. Whole brains were collected and SEH gene (EPHX2) mRNA and SEH protein expression were analyzed using RT-PCR and Western blot, respectively. mRNA levels were relatively decreased in high-salt rats (1.0 ± 0.32 NS vs 0.39 ± 0.07 HS, n=6). However, …
Physiologically-Based Pharmacokinetic Modeling For Predicting Drug-Drug Interactions,
2011
San Jose State University
Physiologically-Based Pharmacokinetic Modeling For Predicting Drug-Drug Interactions, David M. Ng, Ali Navid
STAR Program Research Presentations
Dynamics of interactions between the drugs caffeine and ciprofloxacin are predicted using physiologically-based pharmacokinetic (PBPK) modeling. Pharmacokinetic means the model determines where the drugs are distributed in the body over time. Physiologically-based means the anatomy and physiology of the human body is reflected in the structure and functioning of the model. Multiple drugs can interact to increase or decrease their beneficial and/or undesired effects. This is important because some common substances, such as caffeine in coffee and soft drinks, are actually drugs that affect the body. By implementing the model as a computer program, it is relatively straightforward to perform …
Interspecies Comparison Of Αii-Spectrin Abundance Between Chinook Salmon And Steelhead,
2011
Western Washington University
Interspecies Comparison Of Αii-Spectrin Abundance Between Chinook Salmon And Steelhead, Brielle D. Kemis, Ann L. Miracle, Katie A. Wagner, Christa M. Woodley
STAR Program Research Presentations
Salmonids, such as Chinook salmon (Oncorhynchus tshawytscha) and steelhead (O. mykiss), are a staple economic, recreational, tribal, and environmental resource, yet many populations are unsustainable. This study was part of a broad scale effort to monitor the impact of downstream migration obstacles on juvenile salmonid health and survival, which is an essential step towards increasing Smolt-to-Adult Return ratios (SARs). The objective of this study was to determine if juvenile Chinook salmon and steelhead exhibit differing quantities of alphaII-Spectrin Breakdown Products (SBDPs) over two consecutive spring migration periods, indicative of neurogenesis rate and/or biological response to head …
The Proteomic Response Of Ciona Intestinalis To Ocean Acidification And Acute Heat Stress: Impacts Of Global Climate Change In Coastal Marine Systems,
2011
California Polytechnic State University, San Luis Obispo
The Proteomic Response Of Ciona Intestinalis To Ocean Acidification And Acute Heat Stress: Impacts Of Global Climate Change In Coastal Marine Systems, Michael Dennis Dwyer Jr.
Biological Sciences
No abstract provided.
Regulation Of Endothelial Nitric Oxide Synthase In Pulmonary Myofibroblasts,
2011
Western Kentucky University
Regulation Of Endothelial Nitric Oxide Synthase In Pulmonary Myofibroblasts, Jonathan David Faughn
Masters Theses & Specialist Projects
Idiopathic pulmonary fibrosis (IPF) is an interstitial lung disease leading to decreased lung volume and eventual respiratory failure. At present, the median post-diagnosis lifespan is between three and six years. Myofibroblasts are collagen-secreting cells essential for wound healing, but also implicated in the fibroproliferation and extra cellular matrix deposition commonly seen in IPF. The nitric oxide (NO) signaling pathway is implicated in protomyofibroblast to myofibroblast transition and regulation. Previous work has shown that in pulmonary myofibroblasts, endothelial nitric oxide synthase (eNOS) is the primary NOS isoform expressed. The current study used cultured rat pulmonary myofibroblasts between passages two and five …
The Role Of The Androgen Receptor Cofactor P44/Wdr77 In Astrocyte Activation,
2011
University of Texas Graduate School of Biomedical Sciences at Houston
The Role Of The Androgen Receptor Cofactor P44/Wdr77 In Astrocyte Activation, Bryce H. Vincent
Dissertations and Theses (Open Access)
Astrogliosis is induced by neuronal damage and is also a pathological feature of the major aging-related neurodegenerative disorders. The mechanisms that control the cascade of astrogliosis have not been well established. In a previous study, we identified a novel androgen receptor (AR)-interacting protein (p44/WDR77) and found that it plays a critical role in the control of proliferation and differentiation of prostate epithelial cells. In the present study, we found that deletion of the p44 gene in the mouse brain caused accelerated aging with dramatic astrogliosis. The p44/WDR77 is expressed in astrocytes and loss of p44/WDR77 expression in astrocytes leads to …
Embryonic Development Of The Grass Shrimp, Palaemonetes Pugio, And The Influence Of Salinity On Cardiac Physiology,
2011
University of Nevada, Las Vegas
Embryonic Development Of The Grass Shrimp, Palaemonetes Pugio, And The Influence Of Salinity On Cardiac Physiology, Amie Lynn Romney
UNLV Theses, Dissertations, Professional Papers, and Capstones
The grass shrimp, Palaemonetes pugio, inhabit the brackish waters along the Atlantic coast of North America and are a key component of the estuarine ecosystem. These decapod shrimp can tolerate large fluctuations in environmental parameters including daily and seasonal fluctuations in salinity between 0 to 55 parts per thousand (seawater at 30-32 ppt). Any observed distribution patterns of adult P. pugio in relation to salinity may be accounted for by their relative ability to tolerate a range in salinity and their ability to maintain internal water volumes and cardiac performance in the earliest life stage, the embryonic period. This thesis …
Proceedings Of The Ii International Meeting In Exercise Physiology,
2011
Western Kentucky University
Proceedings Of The Ii International Meeting In Exercise Physiology
International Journal of Exercise Science: Conference Proceedings
Proceedings of the II International Meeting in Exercise Physiology. Meeting held May 5-7, 2011. This meeting was promoted by the Exercise Physiology Laboratory / UFSCar, in collaboration with UNICAMP and UNIMEP universities. The event was designed to bring Brazilian scientists, especially from São Paulo state that work with Exercise Physiology and Health. The main objective was to put together these students and scientists interested in developing joint researches in this area.
Opioid Addiction Treatments During Pregnancy And Their Effects On Axonal Growth And Myelination In The Developing Central Nervous System,
2011
Virginia Commonwealth University
Opioid Addiction Treatments During Pregnancy And Their Effects On Axonal Growth And Myelination In The Developing Central Nervous System, Manisha Magar
Theses and Dissertations
Treatment with buprenorphine represents a promising alternative for pregnant opioid addicts but there is a need to understand potential effects on nervous system development. We previously showed effects of perinatal exposure to buprenorphine on axonal caliber and myelination in 26-day-old rat corpus callosum. These changes, detected at the end of rapid brain myelination and accompanied by earlier oligodendrocyte maturation, suggested interference with mechanisms coordinating axonal growth and myelination. To better understand buprenorphine actions and to establish whether these effects extend to the spinal cord, we analyzed the corpus callosum and corticospinal tract at 16 days of age, just before the …
Programming,
2011
Western Kentucky University
Programming
International Journal of Exercise Science: Conference Proceedings
Programming for the II International Meeting in Exercise Physiology
Exercise-Induced Lymphocyte Apoptosis And Relationship With Level Of Lactate In Smokers And Non-Smokers,
2011
Texas A&M International University
Exercise-Induced Lymphocyte Apoptosis And Relationship With Level Of Lactate In Smokers And Non-Smokers, Kyung-Shin Park, Yang Lee
International Journal of Exercise Science
Purposes of this study were 1) to examine the exercise intensity where lymphocyte apoptosis index (AI) is significantly increased in smokers and non-smokers, 2) to find out whether AI is associated with level of lactate (L). METHODS: Fourteen healthy untrained smokers (≥ 1 pack year, n=7) and non-smokers (n=7) aged 18 to 26 were recruited. Each subject conducted three treadmill runs at different intensities randomly. Running distance for all three runs was equivalent to 30 minute run at 70% VO2max. AI and L were analyzed at rest (Pre), immediately after (Post), and 1 h following (1 h post) each run. …
Presence Of Observers Increases One Repetition Maximum In College-Age Males And Females,
2011
Samford University
Presence Of Observers Increases One Repetition Maximum In College-Age Males And Females, Steven C. Baker, Alan P. Jung, John K. Petrella
International Journal of Exercise Science
Purpose: To determine if the presence of observers affects muscular strength performance in college-age males and females. Methods: Twenty-five women and 24 men participated in the study. Two counter-balanced trials were performed in which participants completed 1-RM tests in bench press and leg press. During one trial, two members of the opposite gender observed the 1-RM tests. During the other trial there were no observers, other than the tester. Results: 1-RM for females increased 3.4±0.8 lbs on bench press (p<.001) and increased 9.2±3.8 lbs on leg press (p=.025) when observers were present. 1-RM for males increased 4.2±1.1 lbs on bench press (p=.001) and increased 18.8±5.2 lbs on leg press (p=.002) with observers present. There was no difference in the percent increase in 1-RM between males and females for either leg press (p=.71) or bench press (p=.08). Conclusion: College-age males and females lifted more weight during a 1-RM test when observers were present.
Effects Of Backward Walking On Hamstring Flexibility And Low Back Range Of Motion,
2011
A.T. Still University
Effects Of Backward Walking On Hamstring Flexibility And Low Back Range Of Motion, Chet R. Whitley, Janet S. Dufek
International Journal of Exercise Science
The purpose of the study was to examine the effects of backward walking on hamstring flexibility and low back range of motion. Ten healthy female volunteers (29.9±10.0 yr; 165.1±8.2 cm; 68.53±18.4 kg) completed pre-post laboratory testing surrounding a 4-week intervention of backward walking. During the pretest, each participant walked forward on a treadmill at a preferred velocity for 3-5 min. A biaxial electrogoniometer was secured externally to the low back and a sit-and-reach test was performed. Each participant then walked backward at their preferred pace on a treadmill for 10 min, during which time low back motion data were obtained …
Intermittent Vs Continuous Graded Exercise Test For Vo2max In College Soccer Athletes,
2011
Barry University
Intermittent Vs Continuous Graded Exercise Test For Vo2max In College Soccer Athletes, Constance M. Mier, Ryan P. Alexander
International Journal of Exercise Science
The purpose of this study was to determine if a 1-min active recovery between stages during a graded exercise test (GXT) would result in a higher stage intensity and maximum oxygen uptake in college soccer athletes. Eleven athletes completed two GXT protocols on separate days. Each GXT consisted of 2-min stages performed at a constant running speed and incremental increases of 2.5% grade. One GXT was performed continuously and the other was intermittent with 1-min active recovery between each stage. Tests were performed to volitional fatigue. Following the each GXT and a 10-min active recovery period, participants performed a verification …
Accuracy Of Consumer Grade Bioelectrical Impedance Analysis Devices Compared To Air Displacement Plethysmography,
2011
Linfield College
Accuracy Of Consumer Grade Bioelectrical Impedance Analysis Devices Compared To Air Displacement Plethysmography, Janet T. Peterson, Wendy E. S. Repovich, Carlye R. Parascand
International Journal of Exercise Science
The purpose of this study was to examine the accuracy of percent body fat estimates from seven different bioelectrical impedance analysis (BIA) models and a seven-site skin fold formula (SKF) compared with air displacement plethysmography (ADP) in females. Eighty-two female volunteers ranging from 19-67 years (31.96 ± 1.39) enrolled in this study. Body composition was assessed by seven site skin folds (SKF), ADP, Tetrapolar BIA (TBIA), and five consumer grade BIA devices: finger to finger (FF), hand to hand (HH) and three different leg to leg (LL1, LL2, LL3) models. In addition, LL3 was analyzed using the athletic (LL3a) and …
Does The Friel Anaerobic Threshold Test Accurately Detect Heart Rate Deflection In Trained Cyclists?,
2011
University of Kansas Medical Center
Does The Friel Anaerobic Threshold Test Accurately Detect Heart Rate Deflection In Trained Cyclists?, Willie K. Yuen, Shad R. Schreiner, Donald L. Hoover, Janice K. Loudon, Sandra A. Billinger
International Journal of Exercise Science
The Friel Anaerobic Threshold Test (FATT) has been used to determine anaerobic threshold (AT). The FATT suggests AT occurs near the heart rate deflection point (HRDP) at a rating of perceived exertion (RPE) of 17. Purpose: The primary purpose of this study was to determine 1) whether the HRDP could be determined using the FATT, 2) examine differences between HRVT and HR that coincided Borg’s rating of perceived exertion (RPE) of 17, and 3) if riding position (hoods or aero) would influence performance. Methods: Fourteen male cyclists (30.4 ± 7.41years of age; 151.8 ± 60.4 cycled miles/week) participated in the …
Quantitative Analysis Of Contactin-Associated Protein And Voltage-Gated Sodium Channel Isoform 1.6 Following Experimental Diffuse Traumatic Brain Injury,
2011
Virginia Commonwealth University
Quantitative Analysis Of Contactin-Associated Protein And Voltage-Gated Sodium Channel Isoform 1.6 Following Experimental Diffuse Traumatic Brain Injury, Daniel Gardiner
Theses and Dissertations
Traumatic axonal injury (TAI) contributes to the mortality and morbidity following diffuse traumatic brain injury (TBI). Previous work has shown that following TBI, alterations in the molecular domains of axons result in TAI. It is currently posited that injury induced ionic flux is responsible for activating deleterious proteolytic cascades, resulting in altered distributions of axonal components. However, the underlying mechanism of this progressive pathology remains elusive. This study further explores the hypothesis that altered molecular domains contributes to the progressive intra-axonal changes that characterize TAI. Using a rodent model of impact acceleration TBI we examined the expression of nodal and …
Calcineurin Is Required For Trpv1-Induced Ltd Of Ca1 Stratum Radiatum Interneurons,
2011
Brigham Young University - Provo
Calcineurin Is Required For Trpv1-Induced Ltd Of Ca1 Stratum Radiatum Interneurons, Tyron Deray Jensen
Theses and Dissertations
Learning and memory in the brain are thought to be dependent on synaptic plasticity. In response to sensory input, synapses can be strengthened or weakened, known as long-term potentiation or long-term depression (LTD), respectively. Transient receptor potential vanilloid 1 (TRPV1) has been shown to mediate a novel form of presynaptic LTD in hippocampal interneurons. TRPV1 is currently being heavily studied in the PNS and being targeted by pharmaceuticals for its anti-nociceptive and anti-inflammatory properties. However, much less is known regarding TRPV1 function in the CNS, including the signal mechanism mediating hippocampal LTD despite its obvious importance. Here we performed whole-cell …
Receptor Influences In Girk Current Activation And Desensitization,
2011
Virginia Commonwealth University
Receptor Influences In Girk Current Activation And Desensitization, Gyu Park
Theses and Dissertations
G protein-coupled receptors (GPCRs) are seven-transmembrane domain receptors that sense extracellular signal and activate intracellular signaling pathways. Metabotropic glutamate receptor 2 (mGluR2) is one of the GPCRs coupled to Gi/o proteins whose Gβγ subunits stimulate G protein-gated inwardly rectifying K+ channels (GIRKs). Previous experiments demonstrated that in planar lipid bilayer both active forms of G proteins [Gα (GTPγS-stimulated) and Gβγ subunits] were required to activate GIRK channels in the absence of the receptor, but surprisingly, the Gβγ subunit alone could activate GIRK channel in the presence of GPCR. Currently, it is not clear whether GPCRs play a role beyond catalyzing …
