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Growth Of Tropical Dasyatid Rays Estimated Using A Multi-Analytical Approach, Owen R. O'Shea, Matias Braccini, Rory McAuley, Conrad W. Speed, Mark G. Meekan 2013 Murdoch University, Perth, Western Australia

Growth Of Tropical Dasyatid Rays Estimated Using A Multi-Analytical Approach, Owen R. O'Shea, Matias Braccini, Rory Mcauley, Conrad W. Speed, Mark G. Meekan

Fisheries Research Articles

We studied the age and growth of four sympatric stingrays: reticulate whipray, Himanutra uarnak (n=19); blue mask, Neotrygon kuhlii (n=34); cowtail, Pastinachus atrus (n=32) and blue-spotted fantail, Taeniura lymma (n=40) rays at Ningaloo Reef, a fringing coral reef on the north-western coast of western Australia. Age estimates derived from band counts within sectioned vertebrae ranged between 1 and 27 years (H. uarnak, 1 - 25 yrs.; N. kuhlii, 1.5 - 13 yrs.; P. atrus, 1 - 27 yrs. and T. lymma, 1 -11 yrs.). Due to limitations of sample sizes, we combined several analytical methods …


The Possible Role Of Brain Angiotensin Ii In The Reduced Growth Of Spontaneously Hypertensive Rats, Daniel R. Jensen, Dr. James P. Porter 2013 Brigham Young University

The Possible Role Of Brain Angiotensin Ii In The Reduced Growth Of Spontaneously Hypertensive Rats, Daniel R. Jensen, Dr. James P. Porter

Journal of Undergraduate Research

The “Spontaneously Hypertensive Rat” strain (SHR) was bred to be innately hypertensive. This hypertension arises in part from over-activity of the brain Renin-Angiotensin system.1 Interestingly, the mean body weight of SHR is significantly reduced compared with the normotensive Wistar-Kyoto strain from which it originates. There are indications that high levels of exogenous Angiotensin II (ANG II) introduced in the brains of normotensive rats inhibit weight gain, and in some cases promote weight loss and/or wasting.2 The mechanism of this effect is not clearly understood, though some studies have shown that high levels of exogenous ANG II can induce anorexia or …


Does Camkii Regulate Exocytosis Through Its Binding To Syntaxin?, Stephen M. Merino, Dr. Dixon Woodbury 2013 Brigham Young University

Does Camkii Regulate Exocytosis Through Its Binding To Syntaxin?, Stephen M. Merino, Dr. Dixon Woodbury

Journal of Undergraduate Research

Neurons use chemical messengers called neurotransmitters to communicate with muscles and other neurons. In the axon terminal of a neuron, neurotransmitters are stored in synaptic vesicles. Neurotransmitter is released from the neuron through a process called exocytosis. In exocytosis, synaptic vesicles fuse with the cell membrane and neurotransmitter is released from the cell. During exocytosis, important proteins in both the synaptic vesicle membrane and cell membrane called SNARE proteins bind to one another. These proteins are required for exocytosis. Exocytosis and subsequent neurotransmitter release is regulated by Ca2+. The mechanism by which Ca2+ regulates exocytosis is poorly understood.


Creating Nutritionist Software To Aid A Poor Community In Northeastern Brazil, Jacob D. Durrant, David D. Busath 2013 Brigham Young University

Creating Nutritionist Software To Aid A Poor Community In Northeastern Brazil, Jacob D. Durrant, David D. Busath

Journal of Undergraduate Research

Virgem dos Pobres is an impoverished community on the banks of lake Mundaú in the Brazilian state of Alagoas. Its dirt roads are lined with houses made of mud and plaster, and sewage is disposed of by means of an open canal that runs through the community’s center. The neighborhood’s population is afflicted with many of the diseases common to extreme poverty: bacterial infections like impetigo, malnourishment, measles, mumps, etc. It was a great privilege to be able to work in some small way to help these wonderful people.


The Luteolytic Potential Of Reduced Doses Of Prostaglandin Administered In The Ischiorectal Fossa (Irf), Jacob Brinkerhoff, Dr. Roy Silcox 2013 Brigham Young University

The Luteolytic Potential Of Reduced Doses Of Prostaglandin Administered In The Ischiorectal Fossa (Irf), Jacob Brinkerhoff, Dr. Roy Silcox

Journal of Undergraduate Research

During the past few years of my education at BYU, I have had the opportunity to study and learn about the dairy industry and what types of management programs are in place to help ensure higher production and lower costs. One of the most important and beneficial reproductive management techniques used on modern dairies is called induced luteolysis or induced destruction of a corpus luteum. This management practice, which can optimize reproductive performance in cattle, involves administration of a hormone called prostaglandin F2µ (PGF).


Botox Cleavage Of Snare Proteins That Are Bound Together In Vitro, Josepp D. Walch, Dr. Dixon Woodbury 2013 Brigham Young University

Botox Cleavage Of Snare Proteins That Are Bound Together In Vitro, Josepp D. Walch, Dr. Dixon Woodbury

Journal of Undergraduate Research

I finished my project last July (2005) just before Dr. Woodbury left for Germany to present his recent work at a few of the academic institutions of Europe. The project commenced in February of 2004.


Snare Regulation, Deborah Adams, Dr. Dixon Woodbury 2013 Brigham Young University

Snare Regulation, Deborah Adams, Dr. Dixon Woodbury

Journal of Undergraduate Research

SNARE proteins are involved in transporting molecules within our cells, but their mechanism of action is not well known (Figure 1). It is established that SNARE proteins aid in the process of vesicles docking at target membranes. When neuronal cells are excited calcium enters the cell and the docked vesicles fuse to the target membrane releasing their contents into the intercellular space called the synapse. It is not exactly known by what method calcium induces membrane fusion but it has been shown that syntaxin and synaptobrevin, or VAMP, are minimal machinery for membrane fusion (1).


Understanding The Pathway Of Steroidogenic Acute Regulatory Protein Using Bioluminescence, Andrew Miner, Dr. Allen Judd 2013 Brigham Young University

Understanding The Pathway Of Steroidogenic Acute Regulatory Protein Using Bioluminescence, Andrew Miner, Dr. Allen Judd

Journal of Undergraduate Research

Steroidogenic Acute Regulatory Protein (StAR) is a protein involved in the genesis of cortisol, testosterone, estrogen and other steroids. In fact, it is the rate-limiting step in the process of steroidogenesis. StAR is responsible for transporting cholesterol from the outside of the mitochondrial matrix to the inside, where it undergoes chemical processes that turn it into the steroids listed above. The body is thought to increase the amount of StAR in mitochondrial membranes when it receives certain signals in order to create more steroids. My topic involves the mechanism for this upregulation.


The Effects During Embryogenesis And Tumorigenesis Of Dominant-Active Beta Catenin In Wnt Signaling, Melissa Schmidt, Dr. Jeffery Richard Barrow 2013 Brigham Young University

The Effects During Embryogenesis And Tumorigenesis Of Dominant-Active Beta Catenin In Wnt Signaling, Melissa Schmidt, Dr. Jeffery Richard Barrow

Journal of Undergraduate Research

Cell communication is crucial in multiceullar organisms. Cells have therefore devised signaling pathways for intracellular communication. One of these is the Wnt signaling pathway. Wnt signaling plays an important role in cell fate decisions during embryogenesis. In the adult, Wnt signaling has been hypothesized to be essential in the maintenance of adult gut, blood, and skin tissues. Interestingly, aberrant Wnt signaling has been found to be the primary defect in all colon cancers and to be the cause of other tumors such as hepatocellular carcinoma (HCC).


Measurement Of Proton Flux Through Influenza A Viral Protein M2, J. Craig Moffat, Matthew A. D'Haenens, David D. Busath, Dr. Dixon J. Woodbury 2013 Brigham Young University

Measurement Of Proton Flux Through Influenza A Viral Protein M2, J. Craig Moffat, Matthew A. D'Haenens, David D. Busath, Dr. Dixon J. Woodbury

Journal of Undergraduate Research

The Influenza A viral protein M2 is known to conduct protons across viral, Golgi, and cell membranes. M2 allows acidification of the interior of the virion thus facilitating viral RNA uncoating. In the active form, M2 is a homotetramer. Each subunit consists of 97 amino acid residues with only one membrane-spanning region of 19 residues. The ion channel activity of M2 has been studied at physiological pH in cellular systems. It is also known that the channel is sensitive to the anti-influenza drug amantadine. Understanding M2 activity at pH 7 will give us greater knowledge of viral function, pharmacological sensitivities, …


Vesicle Fusion: A Size Dependant Factor? The Role Of Ultrasound In Manipulating Vesicle Size, Eric Richardson, Dr. Dixon Woodbury 2013 Brigham Young University

Vesicle Fusion: A Size Dependant Factor? The Role Of Ultrasound In Manipulating Vesicle Size, Eric Richardson, Dr. Dixon Woodbury

Journal of Undergraduate Research

Vesicles are microscopic spherical capsules that have many important applications in both medicine and biology. They are the cell’s primary vehicles for transporting proteins and other chemicals to and from organelles. In the last half century, artificial vesicles (AVs) have been manufactured to explore many biological mechanisms. More recently, AVs have been used as a drug-delivery tool on a microscopic level. This is a promising technique to fight life-threatening diseases such as cancer.


Determining Treatment Of Children Suffering From Protein-Energy Malnutrition, Mary Ann Zarkou, Dr. Bruce Woolley 2013 Brigham Young University

Determining Treatment Of Children Suffering From Protein-Energy Malnutrition, Mary Ann Zarkou, Dr. Bruce Woolley

Journal of Undergraduate Research

Severe malnutrition is one of the most devastating health problems in the world, particularly in developing countries. Protein-energy malnutrition (PEM) is one of the most prevalent types of malnutrition, and is divided into three major types: Kwashiorkor, Marasmus, and Marasmic-kwashiorkor. These diseases in children cause stunted growth, many physiological problems, and lead to increased risk for developing chronic diseases and increased morbidity rates. No effective treatment has been discovered.


Natural Genetic Variation Affecting Calcium Homeostasis, Rebecca Anna Replogle 2013 Purdue University

Natural Genetic Variation Affecting Calcium Homeostasis, Rebecca Anna Replogle

Open Access Dissertations

Calcium (Ca) is essential for multiple functions within the body including skeletal health. The level of Ca in the serum is tightly regulated. During periods of habitual low Ca intake, the body senses a decrease in serum Ca and increases renal conversion of 25 hydroxyvitamin D (25(OH)D) to 1,25 dihydroxyvitamin D (1,25(OH)2D). 1,25(OH)2D acts through the vitamin D receptor (VDR) to increase intestinal Ca absorption, renal Ca reabsorption and skeletal Ca resorption. Efficient intestinal Ca absorption, especially during periods of low Ca intake, is critical for protecting bone mass. Ca absorption and its primary regulator, 1,25(OH)2D, are affected by both …


Effects Of Nitrite And Oxygen On Angiogenesis In Vascular Networks Of The Chicken Embryo, Michael Connery 2013 Virginia Commonwealth University

Effects Of Nitrite And Oxygen On Angiogenesis In Vascular Networks Of The Chicken Embryo, Michael Connery

Theses and Dissertations

Nitric oxide (NO) is an important mediator of angiogenesis and is primarily produced endogenously through the action of nitric oxide synthase (NOS). An alternate pathway for NO production is the conversion of nitrite to NO, which depends on the presence of hemoglobin (Hb) and hypoxic conditions. The angiogenic effects of topically applied sodium nitrite on two vascular beds in the ex ovo chicken embryonic model of angiogenesis were assessed. Gas barrier films were used to modulate local oxygen levels in the chorioallantoic membrane (CAM), a respiratory vascular network, and the area vasculosa (AV) on the yolk sac, a typical peripheral …


Developing A New Fluctuating-Charge Force Field For Charmm, Soren Harward, David Busath 2013 Brigham Young University

Developing A New Fluctuating-Charge Force Field For Charmm, Soren Harward, David Busath

Journal of Undergraduate Research

Molecular Dynamics (hereafter abbreviated MD) is a method of simulating on a computer the behavior of molecules. For over twenty years, it had been a valuable tool that has allowed chemists and physicists to observe chemical interactions that would are difficult or impossible to measure with standard laboratory equipment. The rapid growth of computing power has made feasible simulations of larger and larger systems, so that now biologists can use MD to study the complex chemistry of living organisms.


Erythrocyte Shape Change And Vesicle Release With Methyl-Beta-Cyclodextrin, Jeremy Fairbourn, Dr. John Bell 2013 Brigham Young University

Erythrocyte Shape Change And Vesicle Release With Methyl-Beta-Cyclodextrin, Jeremy Fairbourn, Dr. John Bell

Journal of Undergraduate Research

The study of membrane properties has direct applications in many branches of science. These properties influence the shape and behavior of the cell. With respect to erythrocytes, the membrane becomes susceptible to the action of secretory Phospholipase A2 (sPLA2) following treatment with ionophores such as ionomycin. The changes produced by ionomycin are experimental analogs to occurrences in the body. The normal actions of the immune system and metabolism cause changes to the cells such that apoptosis is eventually the endpoint of the cell.


Influenza A Virus M2 Protein Selectivity And Permeability Of Ammonium Ions At Varying Ph, Jared Kevin Pearson, David D. Busath 2013 Brigham Young University

Influenza A Virus M2 Protein Selectivity And Permeability Of Ammonium Ions At Varying Ph, Jared Kevin Pearson, David D. Busath

Journal of Undergraduate Research

The M2 protein inside the Influenza A virus capsid is vital to the virus’ replication. When the virus enters a cell, the M2 protein forms ion channels that allow the viral RNA to dissociate from stabilizing proteins, enter the host cell nucleus, and replicate. Blocking the formation of these ion channels, or disabling M2 protein ion transport therefore is an important area of research; inactivating the channel will prevent Influenza A replication.


The Effects Of Interleukin-1, Interleukin-6, And Tumor Necrosis Factor On Aldosterone Release From The Bovine Adrenal Cortex, Todd A. Richards, Dr. Allan M. Judd 2013 Brigham Young University

The Effects Of Interleukin-1, Interleukin-6, And Tumor Necrosis Factor On Aldosterone Release From The Bovine Adrenal Cortex, Todd A. Richards, Dr. Allan M. Judd

Journal of Undergraduate Research

The adrenal is an endocrine gland that is important for the release of steroid hormones and cathecholamines. The adrenal gland is closely associated with the immune system. This association is closely linked by the release of cytokines. Cytokines are a diverse group of proteins first isolated within the immune system but have now been identified in numerous tissues, including those of the endocrine system. The principle cytokines affecting the adrenal gland are interleukin-1 (IL-alpha and IL-beta), interleukin-6 (IL-6) and tumor necrosis factor (TNF). These cytokines can regulate adrenal function, particularly during increased stress and disease, and because they are produced …


Degeneration Of Articular Cartilage In The Temporomandibluar Joint Of Mice With Disproportionate Micromelia (Dmm), Robert R. Seegmiller 2013 Brigham Young University

Degeneration Of Articular Cartilage In The Temporomandibluar Joint Of Mice With Disproportionate Micromelia (Dmm), Robert R. Seegmiller

Journal of Undergraduate Research

Osteoarthritis is a common degenerative joint disease affecting humans today. This disease leads to premature degradation of articular cartilage in joints, such as the knee, and it can be caused by mutations in collagen coding genes. Collagen is the basic building block of cartilage, so any mutations would affect the response of collagen to outside factors.


Type Iia Procollagen – Osteoarthritis Biomarker In Dmm Mice, John McAllister, Kenneth Merrell, Dr. Robert E. Seegmiller 2013 Brigham Young University

Type Iia Procollagen – Osteoarthritis Biomarker In Dmm Mice, John Mcallister, Kenneth Merrell, Dr. Robert E. Seegmiller

Journal of Undergraduate Research

Arthritis is a painful, debilitating disease that affects sixty-six million Americans (The Arthritis Foundation 2005). Osteoarthritis (OA), results in joint degeneration caused by cartilage erosion and synovial inflammation. While the causes of OA remain unclear, current evidence suggests that both mechanical and biochemical factors can initiate OA. Ethical and practical issues limiting the availability of human tissue in OA research necessitate the use of animal models. Mice with the disproportionate micromelia mutation (Dmm) exhibit histological characteristics similar to those of humans with OA. Use of Dmm as a model of OA may lead a discovery of a common mechanism associated …


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