Physiology Commons^™

Co-Ingestion Of Carbohydrate With Branched-Chain Amino Acids Or L-Leucine Does Not Preferentially Increase Serum Igf-1 And Expression Of Myogenic-Related Genes In Response To A Single Bout Of Resistance Exercise, Rui Li, Maria Pontes Ferreira, Matthew B. Cooke, Paul La Bounty, Bill Campbell, Mike Greenwood, Darren S. Willoughby, Richard B. Kreider

Nutrition and Food Science Faculty Research Publications

This study determined if the co-ingestion of carbohydrate (CHO) with branched-chain amino acids (BCAA) or L-leucine (LEU) preferentially affected serum IGF-1 and the expression of myogenic-related genes in response to resistance exercise (RE). Forty one, college-age males were randomly assigned to 1 of 4 groups: CHO, CHO-BCAA, CHO-LEU, or placebo (PLC). Resistance exercise consisted of 4 sets of leg press and leg extension at 80% 1RM. Supplements were ingested peri-exercise, and venous blood and muscle biopsies were obtained pre-exercise (PRE), and at 30, 120, and 360 min post-exercise. Serum IGF-1 was determined with ELISA, and skeletal muscle mRNA expression of …

Determining The Role Of Il-4 Induced Neuroinflammation In Microglial Activity And Amyloid-Ss Using Bv2 Microglial Cells And App/Ps1 Transgenic Mice, Clare H. Latta, Tiffany L. Sudduth, Erica M. Weekman, Holly M. Brothers, Erin L. Abner, Gabriel J. Popa, Michael D. Mendenhall, Floracita Gonzalez-Oregon, Kaitlyn Braun, Donna M. Wilcock

Physiology Faculty Publications

Background

Microglia are considered the resident immune cells of the central nervous system (CNS). In response to harmful stimuli, an inflammatory reaction ensues in which microglia are activated in a sequenced spectrum of pro- and antiinflammatory phenotypes that are akin to the well-characterized polarization states of peripheral macrophages. A “classically” activated M1 phenotype is known to eradicate toxicity. The transition to an “alternatively” activated M2 phenotype encompasses neuroprotection and repair. In recent years, inflammation has been considered an accompanying pathology in response to the accumulation of extracellular amyloid-β (Aβ) in Alzheimer’s disease (AD). This study aimed to drive an M2a-biased …

Successful Inquiry Based Activities In High School Physiology, Sowmya Anjur

Faculty Publications & Research

Physiology and Disease students at the Illinois Mathematics and Science Academy in Aurora employ inquiry driven activities to improve their understanding and articulation of the discipline. Students make heart models and make correlations with heart rate, lung capacity and blood pressure measurements. Students seemed to gain a better understanding of the cardiovascular unit as reflected in their heart unit assessment scores.

Amp-Activated Protein Kinase Controls Exercise Training - And Aicar-Induced Increases In Sirt3 And Mnsod, Josef Brandauer, Marianne A. Andersen, Holti Kellezi, Steve Risis, Christian Frozig, Sara G. Vienberg, Jonas T. Treebak

Health Sciences Faculty Publications

The mitochondrial protein deacetylase sirtuin (SIRT) 3 may mediate exercise training-induced increases in mitochondrial biogenesis and improvements in reactive oxygen species (ROS) handling. We determined the requirement of AMP-activated protein kinase (AMPK) for exercise training-induced increases in skeletal muscle abundance of SIRT3 and other mitochondrial proteins. Exercise training for 6.5 weeks increased SIRT3 (p < 0.01) and superoxide dismutase 2 (MnSOD; p < 0.05) protein abundance in quadriceps muscle of wild-type (WT; n = 13–15), but not AMPK α2 kinase dead (KD; n = 12–13) mice. We also observed a strong trend for increased MnSOD abundance in exercise-trained skeletal muscle of healthy humans (p = 0.051; n = 6). To further elucidate a role for …

The Biology Of Claudin 6 (Cldn6) In The Developing Mouse Lung, Felix Ruben Jimenez Rondan

Theses and Dissertations

The tight junctions (TJ), which are located in the apical region between epithelial and endothelial cells, regulate the paracellular diffusion of ions and small molecules and play an important role in maintaining cell polarity, cell-cell integrity, and permeability. In the lung, epithelial cells are attached by TJ structures. They provide a permeable barrier and cell communication. The loss of barrier integrity, which is maintained by the expression of claudins (Cldn), results in cellular permibilization and leads to paracellular diffusion of solutes and harmful molecules. There are 27 known Cldn homologous members in mice and human. Cldn6 is mostly expressed in …

The Role Of Receptors For Advanced Glycation End-Products (Rage) And Ceramide In Cardiovascular Disease, Michael Bruce Nelson

Theses and Dissertations

Type 2 diabetes and cigarette smoke exposure are associated with an increased risk of cardiovascular complications. The role of advanced glycation end-products (AGEs) is already well-established in numerous comorbidities including cardiomyopathy. Given the role of AGEs and their receptor, RAGE, in activating inflammatory pathways, we sought to determine whether ceramides could be a mediator of RAGE-induced altered heart mitochondrial function. Using an in vitro model, we treated H9C2 cardiomyocytes with carboxy-methyl lysine-BSA, followed by mitochondrial respiration assessment. We found that mitochondrial respiration was significantly impaired in AGE-treated cells, but not when co-treated with myriocin, an inhibitor of de novo …

Morphological And Moisture Availability Controls Of The Leaf Area-To-Sapwood Area Ratio: Analysis Of Measurements On Australian Trees, Henrique Furstenau Togashi, Iain Colin Prentice, Bradley John Evans, David Ian Forrester, Paul Drake, Paul Feikema, Kim Brooksbank, Derek Eamus, Daniel Taylor

Natural Resources Research Articles

1. The leaf area-to-sapwood area ratio (LA:SA) is a key plant trait that links photosynthesis to transpiration. The pipe model theory states that the sapwood cross-sectional area of a stem or branch at any point should scale isometrically with the area of leaves distal to that point. Optimization theory further suggests that LA:SA should decrease toward drier climates. Although acclimation of LA:SA to climate has been reported within species, much less is known about the scaling of this trait with climate among species.
2. We compiled LA:SA measurements from 184 species of Australian evergreen angiosperm trees. The pipe model was …

Reduced Regional Cerebral White Matter Perfusion In Middle-Aged Hispanic Adults, Evan P. Pasha, Takashi Tarumi, Mitzi M. Gonzales, Miriam E. Pearman, Andrean P. Haley, Hirofumi Tanaka

International Journal of Exercise Science: Conference Proceedings

Background: Hispanics are at least 1.5 times more likely at any age past 55 years to have mild cognitive impairment and manifest dementia symptoms 7 years earlier than non-Hispanic Caucasians. Regional cerebral hypoperfusion has been implicated as an initial event in the pathogenesis of dementia. Aim: To compare cerebral perfusion in brain regions known to be susceptible to vascular related hypoperfusion in Hispanic and non-Hispanic Caucasian adults. Methods: Apparently healthy middle-aged Hispanic (n=16) and non-Hispanic Caucasian (n=16) subjects were matched across age, gender, years of education, and cognitive status. Arterial spin labeled perfusion magnetic resonance imaging (ASL-MRI) estimated cerebral blood …

High-Density Lipoprotein Antioxidant Responses To High-Intensity Interval And Steady-State Moderate-Intensity Exercise, Zacharias Papadakis, Jeffrey S. Forsse, Annie A. Bane, Taylor Kyle, Li Qian, Flor Elisa Morales Marroquín, Peter W. Grandjean

International Journal of Exercise Science: Conference Proceedings

High-intensity interval exercise (HIIE) may impart health benefits beyond what is acquired through moderate-intensity steady state exercise (SSE). Paraoxonase 1 (PON1), an antioxidant associated with high-density lipoprotein (HDL), may be altered with exercise; however, it is unclear whether HIIE provides a greater stimulus for increasing PON1 antioxidant activity than a comparable or greater amount of SSE. PURPOSE: Determine the influence of HIIE on PON1 concentration and activity relative to a comparable amount of moderate-intensity SSE and a dose that is half that of SSE. METHODS: Seventeen male participants (age 27.8 + 6.4 yr; weight 80.6 + 9.0 kg; BMI 25.1 …

The Effects Of Physical Activity On Markers Of Hepatic Inflammation During Weight-Loss, Andrew Seija, Kaylee E. Poole, Kevin Eck, Matthew Harris, Tayler N. Nick, Joshua S. Wooten

International Journal of Exercise Science: Conference Proceedings

Non-alcoholic fatty liver disease (NAFLD) represents a continuum that begins with accumulation of lipid in hepatic cells progressing to hepatic steatosis with inflammation (steatohepatitis), fibrosis, and cirrhosis. Weight-loss using dietary modification and physical activity are common strategies used for the treatment of NAFLD; however, it remains to be determined the effects of physical activity on hepatic inflammation during weight-loss. The purpose of this study was to determine the therapeutic role of physical activity on plasma and hepatic inflammatory markers during weight-loss. Male C57BL/6 mice were fed either a low-fat (LFD; 10% kcal fat) or high-fat (HFD; 60% kcal fat) diet …

The Effects Of Physical Activity On Hepatic Lipid Metabolism During Weight-Loss, Kaylee Poole, Andrew Seija, Kevin Eck, Matthew Harris, Tayler Nick, Joshua S. Wooten

International Journal of Exercise Science: Conference Proceedings

Non-alcoholic fatty liver disease (NAFLD) develops as a result of physical inactivity and overnutrition. Changing dietary behaviors and increasing physical activity are common strategies used for weight-loss; however, it remains unclear what additional benefits are provided by incorporating physical activity in a weight-loss program for the treatment of NAFLD. The purpose of this study was to determine how physical activity reduces hepatic steatosis and changes the expression of hepatic lipogenic genes during weight-loss. Male C57BL/6 mice were fed either a low-fat (LFD; 10% kcal fat) or high-fat (HFD; 60% kcal fat) diet for 10-weeks. Following 10-weeks, the HFD group was …

Effects Of Fish Oil Supplementation On Post-Resistance Exercise Muscle Soreness In Untrained Females, Grant M. Tinsley, Joshua J. Gann, Stefan R. Huber, Paul M. La Bounty, Rodney G. Bowden, Thomas L. Andre, Paul M. Gordon, Peter W. Grandjean

International Journal of Exercise Science: Conference Proceedings

The anti-inflammatory properties of omega-3 fatty acids contained in fish oil may help alleviate symptoms of delayed onset of muscle soreness (DOMS) and improve recovery from exercise.

PURPOSE: To examine the effects of fish oil supplementation on the time-course of post-resistance exercise muscle soreness in young untrained females.

METHODS: Seventeen non-resistance trained females (age 23.6 ± 3.0 y, % fat 27.4 ± 3.2, weight 60.2 ± 9.6 kg) were randomized into one of two groups: fish oil (6 g/d; 5:1 EPA:DHA) or placebo (6 g/d corn/soy oil). After consuming the supplements for one week, participants underwent a single bout of …

Controlled Cold Water And Water Slushy Ingestion, And Heat Performance In Subjects Of Average Fitness, Samantha N. Cantu, Christopher M. Hearon, Matthew V. Bliss, Ryan Fiddler

International Journal of Exercise Science: Conference Proceedings

Fluid ingestion is known to improve exercise performance and could lead to a heat sink effect, if cold enough. While research has been conducted on the influence of hydration in exercise performance, little has been done which consider beverages’ temperature during controlled consumption. PURPOSE: To examine the effect of controlled consumption of water at different temperatures on heat performance in subjects of average fitness. METHODS: Fifteen males, ages 18-29, with no prior heat illness were recruited. Subjects were tested for body composition and peak oxygen consumption (VO_2peak) prior to testing. All subjects underwent three experimental trials [cold …

Effect Of Nitric Oxide Bioavailability On Cutaneous Microvascular Function In Healthy Young African Americans, Kiyoung Kim

International Journal of Exercise Science: Conference Proceedings

We have recently demonstrated impaired microcirculatory function in healthy young African Americans (AAs) as indexed by attenuated cutaneous vasodilation during local heating. The mechanisms resulting in this impairment are multifactorial; however, recent evidence suggests that decreased nitric oxide (NO) bioavailability is a primary contributor. PURPOSE: To expand our previous findings and determine if this reduced microvascular vasodilatory response in AAs is related to a decrease in NO bioavailability. We hypothesized that NO dependent cutaneous vasodilation in response to local heating is lower in healthy young AAs relative to age / sex / body mass matched Caucasians (CA). METHOD: To date …

3-Nitrotyrosine And Soluble Vascular And Intracellular Adhesion Molecule Responses To High-Intensity Interval And Steady-State Moderate-Intensity Exercise, Jeffrey S. Forsse, Zacharias Papadakis, Annie A. Bane, J. Kyle Taylor, Li Qian, Flor Elisa Morales Marroquín, Peter W. Grandjean

International Journal of Exercise Science: Conference Proceedings

Vascular endothelium may respond differently to high-intensity interval exercise (HIIE) when compared to moderate-intensity steady state exercise (SSE). We hypothesized that greater sympathetic stimulation of soluble vascular adhesion molecule-1 (sVCAM-1) and intracellular adhesion molecule-1 (sICAM-1) and greater oxidative and nitrative stress on the vascular endothelium may transiently result from HIIE. PURPOSE: Determine the influence of HIIE on sVCAM-1, sICAM-1 and 3-nitrotyrosine (NT), a marker of nitric oxide-dependent reactive nitrogen species and nitrative stress, relative to a comparable amount of moderate-intensity SSE and a dose that is half that of SSE. METHODS: Seventeen male participants (age 27.8 + 6.4 yr; weight …

The Positive Effects Of Acute Resistance Exercise On Blood Lipid Profiles In College-Aged Men, Farzana F. Alam, Jin K. Park, Neil A. Schwarz, Ashley P. Lamar, Yunsuk Koh

International Journal of Exercise Science: Conference Proceedings

Low- to moderate-intensity exercise has been widely recommended for people at any age to improve cardiovascular health due to its positive effects on blood lipids and lipoproteins. Recently, many people have been participating in not only low- to moderate-intensity, but high-intensity exercise as well in order to improve their cardiovascular health. However, it is unclear whether high-intensity exercise, particularly resistance exercise, can positively influence blood lipids and lipoproteins. Purpose: The current study examined the effects of low- and high-intensity of resistance exercise on changes in blood lipids and lipoproteins. Methods: In a randomized, cross-over design, 10 healthy recreationally resistance-trained (at …

Changes In Creatine Phosphokinase Isoenzymes After Varying Levels Of Resistance Exercise, Ashley Lamar, Yunsuk Koh, Jin K. Park, Farzana Alam

International Journal of Exercise Science: Conference Proceedings

Creatine phosphokinase (CK) isoenzymes are found in various tissues, such as skeletal muscle (CK-MM), cardiac muscle (CK-MB), and the brain (CK-BB). Elevated CK isoenzymes indicate damage or injury to specific tissue. Using CK isoenzymes as a marker in order to indicate muscle damage as a result of different degrees of resistance exercise has not been clearly examined. Purpose: To determine the changes in CK isoenzymes following resistance exercise at different intensities. Methods: Ten healthy recreationally resistance-trained (at least 3 - 6 days per week for a minimum of one year) men participated in the study. The participants performed …

Leg Lean Mass Adaptations Following Short-Term Barbell Training In Women, Jacob A. Mota, Matt S. Stock, Kendra D. Olinghouse, Alexander S. Drusch, Brennan J. Thompson

International Journal of Exercise Science: Conference Proceedings

Participation in a resistance training program results in both neural adaptations and increases in lean body mass, the latter of which is considered a slow process. The majority of previous investigations that have examined the time course associated with muscular adaptations have utilized exercise machines or single-joint movements. Furthermore, the adaptations associated with resistance training in women are not as well understood as those for men. The purpose of this investigation was to examine the effects of a four week resistance training intervention on changes in leg lean mass in untrained, college-aged women. Thirty-five women (mean ± SD age = …

Changes In Arterial Stiffness And Blood Pressure During Various Vascular Reactivity Tests, Jisok Lim, Miriam Pearman, Mohammed Alkatan, Daniel Machin, Wonil Park, Hirofumi Tanaka

International Journal of Exercise Science: Conference Proceedings

Increased arterial stiffness is an independent prognosis marker of elevated cardiovascular risks. However, some investigators have questioned the utility of arterial stiffness above and beyond the traditional blood pressure (BP) measurement as the change in arterial stiffness is often accompanied by the corresponding change in BP. Although the associations between chronic levels of arterial stiffness and BP have been fairly well studied, it is not clear if and how much arterial stiffness is influenced by acute changes in BP. PURPOSE: We determined magnitudes of BP-dependence of arterial stiffness during acute BP perturbations. METHODS: Thirty apparently healthy subjects (46±4 …

Passive Hyperthermia Therapy Similarly Reduces Arterial Blood Pressure In Obese Type 2 Diabetic And Healthy Individuals, Eric Rivas

International Journal of Exercise Science: Conference Proceedings

Diabetes which is associated with impaired glycemic control, can damage macro and microvasculature, and impair vascular relaxation (i.e., endothelial dysfunction). The high thermal conductance of hot water will cause thermoregulatory adjustments that increase cardiac output, heart rate and stroke volume while reducing central venous pressure. This project tested the hypothesis that an acute bout of hot water hyperthermia will reduce arterial blood pressure during and following the exposure, but the response will be attenuated in obese type 2 diabetics. Eleven obese type 2 diabetics (T2D) (50.1 ± 12 y, 45 ± 7.1% fat mass, 7.5 ± 1.8% HbA1C) and nine …