Physiology Commons^™

Tissue Factor Expression, Regulation, And Signaling In Human Airway Cells, Michael D. Davis

Theses and Dissertations

Rationale: Tissue Factor (TF) is a transmembrane glycoprotein that canonically functions as the initiator of the coagulation cascade. Increased levels of TF have been associated with inflammatory airway diseases. Since lipopolysaccharide (LPS) is known to elicit and inflammatory response in airway epithelium, we hypothesized that airway epithelial cells release TF when exposed to LPS. Since TF aids in local wound healing, we also hypothesized that inhibition of TF would decrease NHBE growth. The specific aim of this work was to evaluate the effects of LPS exposure on TF production and release from airway epithelia and determine the signaling pathways involved. …

Po2 Dependence Of Oxygen Consumption In Skeletal Muscle Of Diabetic And Non-Diabetic Rats, Alexander C. Liles

Theses and Dissertations

Abstract

PO₂ DEPENDENCE OF OXYGEN CONSUMPTION IN SKELETAL MUSCLE OF DIABETIC AND NON-DIABETIC RATS

By: Alexander C. Liles

A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science at the Medical College of Virginia Campus, Virginia Commonwealth University

Virginia Commonwealth University, 2017

Advisor: Roland N. Pittman, Ph.D.

Department of Physiology and Biophysics

Type 2 diabetes mellitus (T2DM) is a major medical problem around the world, affecting nearly 6% of the world’s population. This study was an attempt to better understand physiological changes the disease may cause to the microcirculation and more specifically, to …

Identification Of Expression And Function Of The Glucagon-Like Peptide-1 Receptor In Gastrointestinal Smooth Muscle, Alexander T. May

Theses and Dissertations

In response to ingestion of nutrients, enteroendocrine L cells secrete the incretin hormone, glucagon-like peptide-1 (GLP-1), to enhance glucose-dependent insulin release. Therapies related to GLP-1 are approved for type 2 diabetes. The GLP-1 receptor (GLP-1R) is expressed in cells of the gastrointestinal tract and elsewhere. In pancreatic beta cells, GLP-1R are coupled to the Gs/cAMP/PKA pathway. The expression and function of GLP-1R in gastrointestinal smooth muscle are not known. Aim. To test the hypothesis that GLP-1 regulates smooth muscle function by acting on GLP-1R expressed on smooth muscle. Methods. Smooth muscle cells (SMC) were isolated and cultured. Expression of GLP-1R …

Effects Of 17Β Estradiol In The Metabolism And Morphology Of Bluegill Sunfish (Lepomis Macrochirus), Neeta Parajulee Karki

Masters Theses

Fish natural habitats are increasingly contaminated with various estrogenic compounds, including 17β estradiol (E2). This compound causes adverse effects on the reproductive system of male fish; however, the effects of E2 on other aspects of fish metabolism, morphology and histopathological changes in internal organs are not well known. The objective of this study is to evaluate the effects of E2 exposure on the basal and stressed metabolic rate, morphological changes in body shapes, and histological changes in the liver tissues of sunfish species. Fish were held individually in ten gallon tanks under two treatments of 40 and 80 ng/L and …

The Role Of Creg1 As A Master Regulator Of Liver Function, Abdulrahman Siran Aldaghmi

Masters Theses

The liver is known as the chemical factory of the body because it performs a wide range of biochemical functions required for life. Since the liver has such an important role in regulation of normal physiological processes, liver diseases cause a high rate of morbidity and mortality. Therefore, understanding the mechanisms of liver development will shed light on the causes of liver disease. In this study, a cell line model that utilizes rat hepatoma cells (Fg14) and hepatoma variant cells (H11) was used to identify master regulators of liver gene expression. Whole genome expression studies identified the gene CREG1 (Cellular …

The Influence Of Different Pre-Exercise Routines On Sprint Performance, Rok Mravljak

Masters Theses

Sprint performance can be enhanced by interventions for short-term (acute) purposes and/or long-term purposes. Acute neuromuscular responses are usually achieved by using different pre-exercise routines at the end of the warm-up period. Recently, there have been several studies examining the effects of various pre-exercise routines on sprint performance, yet there has not been a research study designed that compared the three most commonly used pre-exercise routines in professional and recreational sports (static stretching, dynamic stretching and foam-rolling). Therefore, this study investigated and compared the results of static stretching, dynamic stretching, self-myofascial release and the control group, in order to provide …

The Effect Of Repeated Sprint Training In Hypoxia And Beta-Alanine Supplementation On Exercise Performance, Ran Wang

Electronic Theses and Dissertations

The primary objective of this study was to evaluate the synergistic effects of repeated sprint training in hypoxia (RSH) and beta-alanine supplementation on performance in recreationally active men. Participants were randomly assigned to one of the following groups: hypoxia + beta-alanine (HB, n = 10), hypoxia + placebo (HP, n = 9), normoxia + beta-alanine (NB, n = 11) and normoxia + placebo (NP, n = 8). All participants completed a total of 8 training sessions (each consisting of 3 sets of 5 × 10-s sprints at a resistance of 7.5% of body mass, with 20-s rest intervals between sprints) …

Remote Preconditioning: Evaluating The Efficacy Of Cardioprotection In Type-2 Diabetes And Exploring The Mechanistic Role Of Exosomes, Joseph Michael Wider

Wayne State University Dissertations

Remote preconditioning is a promising and robust treatment for myocardial

ischemia/reperfusion injury that evokes cardioprotection through endogenous neural

and/or humoral signaling. A recent study has reported that protective signaling is

mediated by exosomes through the circulation; however this concept is supported by

limited and inconsistent evidence. Despite overwhelming success in preclinical studies,

the efficacy of remote preconditioning in human studies is inconclusive. Importantly, the

majority of remote preconditioning studies use healthy animal models despite growing

evidence that comorbidities, such as type-2 diabetes, may negatively influence

outcomes. Nonetheless, the efficacy of remote preconditioning in the setting of type-2

diabetes has not …

Estimating The Variables Of The Nonlinear Dynamical Theory Of Acute Cell Injury, Fika Tri Anggraini

Wayne State University Dissertations

This Dissertation is the first to estimate the variables D and S of the nonlinear theory of acute cell injury in hippocampal regions CA1 and CA3 using a rat model of global brain ischemia and reperfusion (I/R). D was estimated by quantifying protein aggregates using ubiquitin Western blotting. S was estimated by quantifying changes in polysomal mRNAs as measured by microarray chips. D and S time courses were sampled at 0.5, 8, 16, 24, 48, and 72 hrs of reperfusion after 10 min brain ischemia. CA1 dies and CA3 survives. The theory predicts D will be larger in CA1 and …

The Effects Of Trehalose On Osmotic Tolerance, Membrane Integrity And Quality Of Equine Sperm During Cold Storage And Cryopreservation, Dissaya Srinutiyakorn

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to investigate the protective effects of trehalose on osmotic tolerance and the quality of equine sperm before and after freezing and thawing. Equine ejaculated semen was collected from six stallions (3 ejaculates per stallion; >50% motility and >70% normal morphology). In the present study, the osmotic tolerance limit of equine sperm to osmotic changes was firstly verified, and followed with the protective effects of trehalose on osmotic tolerance of equine sperm. In the study, sperm was exposed to Tyrode's albumin lactate pyruvate (TALP) medium at different osmolalities: isosmolality (300 mOsm/kg; control) and anisosmolality (150, 450, 600 and …

The Effects Of Bicycling Exercise On The Metabolic Cost Of Walking In Older Adults, Daniel Hugo Aslan

Cal Poly Humboldt theses and projects

Impaired walking performance is a key determinant of morbidity among older adults. A distinctive characteristic of impaired walking performance among older adults is a greater metabolic cost compared to young adults. Specifically, healthy older adults have been shown to have a 15-20% greater metabolic cost of walking compared to young adults. However, a recent study suggests that older adults who routinely run for exercise have a lower metabolic cost of walking compared to older adults who walk for exercise. Yet, it remains unclear if other aerobic exercises such as bicycling elicits similar improvements on walking metabolic cost among older adults. …

Biomarkers Of Sepsis: A Retrospective Approach, Jose M. Roberts

Williams Honors College, Honors Research Projects

Background: Biomarkers are taking the spotlight in becoming the norm for early diagnoses. Sepsis is an inflammatory disease that increases metabolic rate in children. The first biomarker is hyponatremia. Hyponatremia is a frequent electrolyte imbalance in clinical practice, often observed in children with inflammatory disease and infection. Presence of hyponatremia is associated with electrical signaling imbalances, inflammation and renal dysfunction. The clinical value of hyponatremia in pediatric patients is unknown. The C-reactive protein is a second biomarker. Its presence signifies that necrotic cells and inflammation are present.

Objectives: To evaluate the use of biomarkers in children seen in the Emergency …

Circadian Modulation Of Breathing Stability And Respiratory Plasticity, Mohamad El Chami

Wayne State University Dissertations

Purpose: Our project was designed to determine the effect of time of day on multiple mechanisms influencing breathing stability and respiratory plasticity. We investigated if the number and duration of breathing events coupled to upper airway collapsibility, as well as the carbon dioxide reserve, chemoreflex sensitivity and arousal threshold during non-rapid eye movement (NREM) sleep were affected by the time of day. In addition, we examined if mild intermittent hypoxia (IH) initiates long-term facilitation of upper airway muscle activity leading to a reduction in the therapeutic continuous positive airway pressure required to eliminate breathing events.

Methods: Male participants with obstructive …

The Effects Of Beetroot Juice On Vo2max And Blood Pressure During Submaximal Exercise, Jose M. Perez

College of Graduate Studies: Theses & Dissertations

Supplementation with beetroot juice (BR) has been shown to reduce blood pressure (BP) at rest and improve several performance parameters during submaximal and maximal exercise. BR effects on BP during submaximal exercise have not been investigated. Furthermore, the effects of BR on VO₂max are inconclusive. The purpose of this study was to investigate the effects of BR on VO₂max and BP during submaximal exercise. 20 healthy, recreationally trained volunteers (age 21.8±2.35 years, weight 75.10±10.62 kg, height 177.4±6.39 cm) participated in this study, which had a double-blind placebo controlled randomized crossover design. Participants began supplementation with either …

Dental Topography And Microwear Texture In Sapajus Apella, Peter S. Ungar, Claire L. Hartgrove, Alexa N. Wimberly, Mark F. Teaford

Faculty Publications & Research of the TUC College of Osteopathic Medicine

Dental microwear texture pattern has been associated with aspects of diet for a broad range of mammalian taxa. The basic idea is that soft, tough foods are sheared with a steeper angle of approach between opposing occlusal surfaces, whereas hard, brittle items are crushed with forces perpendicular to those surfaces; and this difference is manifested in anisotropic, striated microwear textures for tough foods, and complex, pitted ones for hard objects. Other factors may, however, influence microwear texture pattern and confound diet signals. For example, if tooth surface slope influences angle of approach between opposing teeth, then perhaps wear-related changes in …

In Vivo Rates Of Dental Microwear Formation In Laboratory Primates Fed Different Food Items, Mark F. Teaford, Peter S. Ungar, Andrea B. Taylor, Callum F. Ross, Christopher J. Vinyard

Faculty Publications & Research of the TUC College of Osteopathic Medicine

No abstract provided.

Moderate-Load Muscular Endurance Strength Training Did Not Improve Peak Power Or Functional Capacity In Older Men And Women, Simon J. Walker, Greg G. Haff, Keijo K. Häkkinen, Robert U. Newton

Research outputs 2014 to 2021

The present study determined the effects of muscular endurance strength training on maximum strength and power, functional capacity, muscle activation and hypertrophy in older men and women. Eighty-one men and women acted as an intervention group while 22 acted as non-training controls (age range 64-75 y). Intervention training included super-sets (i.e., paired exercises, immediately performing the second exercises following completion of the first) with short rest intervals (30-60 s between sets) at an intensity of 50-60% one-repetition maximum (1-RM) for 15-20 repetitions. Concentric leg press actions measured maximum strength (1-RM) and concentric peak power. Functional capacity was assessed by maximum …

The Effects Of Female Genital Mutilation On Women Of Sierra Leone, Nenneh Kalokoh Kalokoh

Walden Dissertations and Doctoral Studies

Female genital mutilation or cutting (FGM/C), a common practice among Sierra Leonean women, carries significant psychological and physical risks. Prior to this study, a substantial need existed for inquiry of the experiences and belief systems within this cultural group to better understand the effects of FGM/C on women and girls. Guided by feminist theory and the theory of cultural relativism, the goal of this phenomenological study was to review the cultural perspectives and experiences of Sierra Leonean women who underwent FGM/C to investigate their concerns about safety and their perceptions of the practices. In addition, this study explored concerns among …

On The Origin Of Superoxide Dismutase: An Evolutionary Perspective Of Superoxide-Mediated Redox Signaling., Adam J. Case

Journal Articles: Cellular & Integrative Physiology

The field of free radical biology originated with the discovery of superoxide dismutase (SOD) in 1969. Over the last 5 decades, a plethora of research has been performed in species ranging from bacteria to mammals that has elucidated the molecular reaction, subcellular location, and specific isoforms of SOD. However, while humans have only begun to study this class of enzymes over the past 50 years, it has been estimated that these enzymes have existed for billions of years, and may be some of the original enzymes found in primitive life. As life evolved over this expanse of time, these enzymes …

Myofilament Calcium Sensitivity: Consequences Of The Effective Concentration Of Troponin I, Jalal K. Siddiqui, Svetlana B. Tikunova, Shane D. Walton, Bin Liu, Meredith Meyer, Pieter P. De Tombe, Nathan Neilson, Peter M. Kekenes-Huskey, Hussam E. Salhi, Paul M.L. Janssen, Brandon J. Biesiadecki, Jonathan P. Davis

Chemistry Faculty Publications

Control of calcium binding to and dissociation from cardiac troponin C (TnC) is essential to healthy cardiac muscle contraction/relaxation. There are numerous aberrant post-translational modifications and mutations within a plethora of contractile, and even non-contractile, proteins that appear to imbalance this delicate relationship. The direction and extent of the resulting change in calcium sensitivity is thought to drive the heart toward one type of disease or another. There are a number of molecular mechanisms that may be responsible for the altered calcium binding properties of TnC, potentially the most significant being the ability of the regulatory domain of TnC to …