Molecular, Genetic, and Biochemical Nutrition Commons^™

Protein Enriched Bread Using Spent Wine Yeast, Zachary Christman

Applied Science Program: Theses

Yeast is a common byproduct of wine production. Wine yeast may be used as a food source. It does not contain bitter and astringent compounds such as those present in spent yeast from making beer The use of nutritional yeast and yeast extract for protein enrichment of bread are presented in this article.

Antibiotic-Induced Gut Dysbiosis And Cognitive, Emotional, And Behavioral Changes In Rodents: A Systematic Review And Meta-Analysis, Shivdeep S. Hayer, Soonjo Hwang, Jonathan B. Clayton

Department of Nutrition and Health Sciences: Faculty Publications

There are previous epidemiological studies reporting associations between antibiotic use and psychiatric symptoms. Antibiotic-induced gut dysbiosis and alteration of microbiota-gut-brain axis communication has been proposed to play a role in this association. In this systematic review and meta-analysis, we reviewed published articles that have presented results on changes in cognition, emotion, and behavior in rodents (rats and mice) after antibiotic-induced gut dysbiosis. We searched three databases—PubMed, Web of Science, and SCOPUS to identify such articles using dedicated search strings and extracted data from 48 articles. Increase in anxiety and depression-like behavior was reported in 32.7 and 40.7 percent of the …

Resistant Potato Starch Supplementation Reduces Serum Histamine Levels In Healthy Adults With Links To Attenuated Intestinal Permeability, Jason R. Bush, Jun Han, Edward C. Deehan, Scott V. Harding, Madhura Maiya, Joshua Baisley, David Schibli, David R. Goodlett

Department of Nutrition and Health Sciences: Faculty Publications

Histamine from our diet or gut microbes can trigger gastrointestinal disturbances, and resistant potato starch (RPS) has previously been shown to alleviate these symptoms while increasing levels of health-associated bacteria such as Akkermansia through unknown mechanisms. Post hoc exploratory metabolomic analysis of serum amino acid, amine, and carnitine metabolites in participants consuming 3.5 g/day RPS or placebo (n = 48) was performed using liquid chromatography-mass spectrometry to determine whether RPS positively influences histamine metabolism and related parameters. Histamine levels were significantly reduced by RPS treatment, but histamine-degrading enzyme products were unaffected by RPS. RPS also reduced histamine-secreting Haemophilus and Lactobacillus …

Fatiguing Joint Angle Does Not Influence Torque And Neuromuscular Responses Following Sustained, Isometric Forearm Flexion Tasks Anchored To Perceptual Intensity In Men, Dolores G. Ortega, Terry J. Housh, Robert Smith, Jocelyn E. Arnett, Tyler J. Neltner, John Paul V. Anders, Richard J. Schmidt, Glen O. Johnson

Department of Nutrition and Health Sciences: Faculty Publications

This study examined the effects of joint angle (JA) on maximal voluntary isometric contraction (MVIC) and neuromuscular responses following fatiguing tasks anchored to RPE. Nine men (mean ± SD: age = 20.7 ± 1.2 yrs) performed forearm flexion MVICs at elbow JAs of 75^o and 125^o before and after sustained, isometric forearm flexion tasks to failure at fatiguing joint angles (FJA) of 75^o and 125^o anchored to RPE = 8. The amplitude and frequency of the electromyographic and mechanomyographic signals were recorded. Neuromuscular efficiency was calculated by dividing normalized torque by normalized electromyographic amplitude. A dependent …

Auricularia Auricula Polysaccharides Attenuate Obesity In Mice Through Gut Commensal Papillibacter Cinnamivorans, Xin Zong, Hao Zhang, Luoyi Zhu, Edward C. Deehan, Jie Fu, Yizhen Wang, Mingliang Jin

Department of Nutrition and Health Sciences: Faculty Publications

Introduction: Auricularia auricula is a well-known traditional edible and medical fungus with high nutritional and pharmacological values, as well as metabolic and immunoregulatory properties. Nondigestible fermentable polysaccharides are identified as primary bioactive constituents of Auricularia auricula extracts. However, the exact mechanisms underlying the effects of Auricularia auricula polysaccharides (AAP) on obesity and related metabolic endpoints, including the role of the gut microbiota, remain insufficiently understood.

Methods: The effects of AAP on obesity were assessed within high-fat diet (HFD)-based mice through obesity trait analysis and metabolomic profiling. To determine the mechanistic role of the gut microbiota in observed anti-obesogenic …

Performance Fatigability And Neuromuscular Responses Are Not Joint Angle Specific Following A Sustained Isometric Forearm Flexion Task Anchored To A High Perceptual Intensity In Women, Jocelyn E. Arnett, Robert W. Smith, Tyler J. Neltner, John Paul V. Anders, Dolores G. Ortega, Terry J. Housh, Richard J. Schmidt, Glen O. Johnson

Department of Nutrition and Health Sciences: Faculty Publications

Objectives: To examine the effects of joint angle (JA) on maximal voluntary isometric contractions (MVIC) and neuromuscular responses following a sustained, isometric forearm flexion task anchored to a rating of perceived exertion (RPE) of 8 (RPE=8). Methods: Nine women (age: 20.7±2.9 yrs; height: 168.8±7.2 cm; body mass: 66.3±6.8 kg) performed 2,3s forearm flexion MVICs at JAs of 75°, 100°, and 125° prior to and following a sustained, isometric forearm flexion task anchored to RPE=8 to task failure (torque reduced to zero) at JA100. Electromyographic (EMG) and mechanomyographic (MMG) signals were recorded from the biceps brachii. Results: The …

Homeobox Transcription Factor Hbxa Influences Expression Of Over One Thousand Genes In The Model Fungus Aspergillus Nidulans, Sandesh S. Pandit, Jinfang Zheng, Yanbin Yin, Sophie Lorber, Olivier Puel, Sourabh Dhingra, Eduardo A. Espeso, Ana M. Calvo

Department of Nutrition and Health Sciences: Faculty Publications

In fungi, conserved homeobox-domain proteins are transcriptional regulators governing development. In Aspergillus species, several homeobox-domain transcription factor genes have been identified, among them, hbxA/hbx1. For instance, in the opportunistic human pathogen Aspergillus fumigatus, hbxA is involved in conidial production and germination, as well as virulence and secondary metabolism, including production of fumigaclavines, fumiquinazolines, and chaetominine. In the agriculturally important fungus Aspergillus flavus, disruption of hbx1 results in fluffy aconidial colonies unable to produce sclerotia. hbx1 also regulates production of aflatoxins, cyclopiazonic acid and aflatrem. Furthermore, transcriptome studies revealed that hbx1 has a broad effect on the A. flavus …

Sesame Eliciting And Safe Doses In A Large Sesame Allergic Population, Liat Nachshon, Joost Westerhout, W. Marty Blom, Benjamin Remington, Michael B. Levy, Michael R. Goldberg, Naama Epstein-Rigbi, Yitzhak Katz, Arnon Elizur

Department of Nutrition and Health Sciences: Faculty Publications

Background: Sesame is a significant food allergen causing severe and even fatal reactions. Given its increasing prevalence in western diet, sesame is listed as an allergenic food requiring labeling in the United States and EU. However, data on the population reaction doses to sesame are limited.

Methods: All sesame oral food challenges (OFCs), performed either for diagnosis or for threshold identification before the beginning of sesame oral immunotherapy (OIT) between November 2011 and July 2021 in Shamir medical center were analyzed for reaction threshold distribution. Safe-dose challenges with 90–120 min intervals were also analyzed.

Results: Two hundred …

Assessment Of The Immune Response In Patients With Insulin Resistance, Obesity, And Diabetes To Covid-19 Vaccination, Jędrzej Warpechowski, Paula Leszczyńska, Dominika Juchnicka, Adam Olichwier, Łukasz Szczerbiński, Adam Jacek Krętowski

Department of Nutrition and Health Sciences: Faculty Publications

The SARS-CoV-19 pandemic overwhelmed multiple healthcare systems across the world. Patients with underlying medical conditions such as obesity or diabetes were particularly vulnerable, had more severe symptoms, and were more frequently hospitalized. To date, there have been many studies on the severity of SARS-CoV-2 in patients with metabolic disorders, but data on the efficiency of vaccines against COVID-19 are still limited. This paper aims to provide a comprehensive overview of the effectiveness of COVID-19 vaccines in individuals with diabetes, insulin resistance, and obesity. A comparison is made between the immune response after vaccination in patients with and without metabolic comorbidities. …

Studies Of The Role Of Mtorc1 In Modulating Intestinal Epithelial Barrier Function, Isaac Adediji

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Mechanistic target of rapamycin complex 1 (mTORC1) signaling impacts intestinal inflammation by modulating the production of cytokines in both intestinal epithelial cells and macrophages. Recent evidence revealed that THP-1-derived macrophages with elevated mTORC1 activity (THP-1shTSC2 macrophages), exhibited increased proinflammatory cytokine production when stimulated with lipopolysaccharide (LPS) compared to counterpart macrophages with repressed or baseline mTORC1 activity. Furthermore, it has been shown that mTORC1 governs intestinal barrier function as evidenced by elevated transepithelial electrical resistance (TEER) and para/transcellular permeability in Raptor-depleted Caco-2 cells (cells with repressed mTORC1 activity) compared to Caco-2 cells with elevated or baseline mTORC1 activity. The present study …

Characteristics Of Yogurt And Culture Bacteria As Affected By Ingredients That Help Treat Leaky Gut, Ricardo Jose Santos Aleman

LSU Doctoral Dissertations

At least 100 million people have leaky gut syndrome. This syndrome is associated with multiple health problems such as diabetes, celiac disease, asthma, multiple sclerosis, and inflammatory bowel disease, costing more than $3 billion per year to treat. L-Glutamine, quercetin, slippery elm bark, marshmallow root, N-acetyl-d-glucosamine (NAG), licorice root, maitake mushrooms, and zinc orotate have been reported to help treat leaky gut.

The objectives were to explore the characteristics of yogurt and culture bacteria as affected by ingredients that help treat leaky gut. In yogurt, the pH, titratable acidity, syneresis, viscosity, color, Streptococcus thermophilus, Lactobacillus bulgaricus, yeast and mold and …

Literature Review—Transthoracic Echocardiography, Computed Tomography Angiography, And Their Value In Clinical Decision Making And Outcome Predictions In Patients With Covid-19 Associated Cardiovascular Complications, Jędrzej Warpechowski, Adam Olichwier, Aleksandra Golonko, Marcin Warpechowski, Robert Milewski

Department of Nutrition and Health Sciences: Faculty Publications

The sudden outbreak of the COVID-19 pandemic posed a great threat to the world’s healthcare systems. It resulted in the development of new methods and algorithms for the diagnosis and treatment of both COVID-19 and its complications. Diagnostic imaging played a crucial role in both cases. Among the most widely used examinations are transthoracic echocardiography (TTE) and computed tomography angiography (CTA). Cardiovascular complications in COVID-19 are frequently associated with a severe inflammatory response, which results in acute respiratory failure, further leading to severe complications of the cardiovascular system. Our review aims to discuss the value of TTE and CTA in …

Diet-Microbiome-Immune Interplay In Multiple Sclerosis: Understanding The Impact Of Phytoestrogen Metabolizing Gut Bacteria, Peter C. Lehman, Sudeep Ghimire, Jeffrey D. Price, Amanda E. Ramer-Tait, Ashutosh K. Mangalam

Department of Nutrition and Health Sciences: Faculty Publications

Multiple sclerosis (MS) is a chronic and progressive autoimmune disease of the central nervous system (CNS), with both genetic and environmental factors contributing to the pathobiology of the disease. Although HLA genes have emerged as the strongest genetic factor linked to MS, consensus on the environmental risk factors is lacking. Recently, the gut microbiota has garnered increasing attention as a potential environmental factor in MS, as mounting evidence suggests that individuals with MS exhibit microbial dysbiosis (changes in the gut microbiome). Thus, there has been a strong emphasis on understanding the role of the gut microbiome in the pathobiology of …

Curated And Harmonized Gut Microbiome 16s Rrna Amplicon Data From Dietary Fiber Intervention Studies In Humans, Cynthia I. Rodriguez, Ali Keshavarzian, Bruce R. Hamaker, Feitong Liu, Genelle R. Lunken, Heather Rasmussen, Hongwei Zhou, Julien Tap, Kelly S. Swanson, Maria Ukhanova, Marion Leclerc, Martin Gotteland, Paola Navarrete, Petia Kovatcheva-Datchary, Wendy J. Dahl, Jennifer B. H. Martiny

Department of Nutrition and Health Sciences: Faculty Publications

Next generation amplicon sequencing has created a plethora of data from human microbiomes. The accessibility to this scientific data and its corresponding metadata is important for its reuse, to allow for new discoveries, verification of published results, and serving as path for reproducibility. Dietary fiber consumption has been associated with a variety of health benefits that are thought to be mediated by gut microbiota. To enable direct comparisons of the response of the gut microbiome to fiber, we obtained 16S rRNA sequencing data and its corresponding metadata from 11 fiber intervention studies for a total of 2,368 samples. We provide …

Compositional Properties Of Flours And Starches From The Philippine National Seed And Industry Council-Registered Root Crops, Edgardo E. Tulin, Chinelo M. Cardaño, Anabella B. Tulin, Ma. Theresa P. Loreto, Ea Kristine Clarisse B. Tulin, Ma. Villa N. Yu

The Philippine Agricultural Scientist

Based in the Visayas State University, Philippines, the germplasm collection of the Philippine Root Crops Research and Training Center (PhilRootcrops) is home to several varieties that have not been fully characterized for their potential use in the production of functional foods. This study evaluated the compositional properties, amylose/amylopectin ratio, and glycemic index of flour and starches of 10 varieties of cassava (Manihot esculenta Crantz), sweet potato (Ipomoea batatas L. (Lam)), and taro (Colocasia esculenta L. Schott); 8 varieties of yam (Dioscorea alata L.); and 5 varieties of arrowroot (Maranta arundinacea L.), which was the recommended …

Validation Of Monitoring Septic Thymus Involution In Mice Using Ultrasound., Jianyao Xue, Xiang-An Li

Journal of Pharmacology & Nutritional Sciences

Sepsis is a dangerous condition commonly seen in the intensive care unit (ICU) of hospitals. It causes the thymus, a crucial immune organ, to shrink. This process is known as thymus involution. Although thymus involution is a natural process that occurs as we age, it is accelerated during sepsis. This process is associated with poor outcomes in septic patients, yet it had never been studied using ultrasonography in a septic mouse model. Researchers from the University of Kentucky have validated a non-invasive ultrasound imaging approach to monitor septic thymus involution in a cecum ligation and puncture (CLP) sepsis mouse model. …

Home-Site Advantage For Host Species–Specific Gut Microbiota, Daniel D. Sprockett, Jeff Price, Anthony Juritsch, Robert J. Schmaltz, Madalena V. F. Real, Samantha L. Goldman, Michael Sheehan, Amanda E. Ramer-Tait, Andrew H. Moeller

Department of Nutrition and Health Sciences: Faculty Publications

Mammalian species harbor compositionally distinct gut microbial communities, but the mechanisms that maintain specificity of symbionts to host species remain unclear. Here, we show that natural selection within house mice (Mus musculus domesticus) drives deterministic assembly of the house-mouse gut microbiota from mixtures of native and non-native microbiotas. Competing microbiotas from wild-derived lines of house mice and other mouse species (Mus and Peromyscus spp.) within germ-free wild-type (WT) and Rag1-knockout (Rag1^−/−) house mice revealed widespread fitness advantages for native gut bacteria. Native bacterial lineages significantly outcompeted non-native lineages in both WT and Rag1 …

The Last Of Us In Therapy: How Mind-Controlling Fungi And Gut Bacteria Affect Your Mental Health, Anastasia Lyon

Journal of Pharmacology & Nutritional Sciences

The "psilocybiome" represents the mutually beneficial relationship between ourselves, our bacteria, and psychedelic drugs. This short review briefly discusses the benefits and limitations surrounding the potential for psychedelic therapy to synergize with gut bacteria to help regulate and maintain proper balance in the immune system, diet, and stress levels. Psychedelic therapy is a novel treatment strategy that has the potential to improve patient mental health, and, by identifying the types of gut bacteria present in patients, it can aid in personalizing medicine by determining how well their "psilocybiome" may respond.

Genome Mining For Anti-Crispr Operons Using Machine Learning, Bowen Yang, Minal Khatri, Jinfang Zheng, Jitender S. Deogun, Yanbin Yin

Department of Nutrition and Health Sciences: Faculty Publications

Motivation: Encoded by (pro-)viruses, anti-CRISPR (Acr) proteins inhibit the CRISPR-Cas immune system of their prokaryotic hosts. As a result, Acr proteins can be employed to develop more controllable CRISPR-Cas genome editing tools. Recent studies revealed that known acr genes often coexist with other acr genes and with phage structural genes within the same operon. For example, we found that 47 of 98 known acr genes (or their homologs) co-exist in the same operons. None of the current Acr prediction tools have considered this important genomic context feature. We have developed a new software tool AOminer to facilitate the improved …

(Pro)Renin Receptor And Its Soluble Form In Metabolic Dysfunction: Friend Or Foe?, Gertrude Arthur

Journal of Pharmacology & Nutritional Sciences

(Pro)renin receptor is component of the renin angiotensin system which has been shown to be involved in several physiological and pathophysiological processes including blood pressure regulation and hypertension, water and electrolyte balance, kidney injury, obesity, and metabolic dysfunction. Enzymatic cleavage of prorenin receptor produces soluble prorenin receptor which can also activate the renin angiotensin system stimulate similar pathophysiological process like its full form receptor. This review explores findings on the role of prorenin receptor and soluble prorenin receptor in metabolic dysfunction and discusses the conflicting findings on soluble prorenin receptor in metabolic dysfunction.