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Molecular, Genetic, and Biochemical Nutrition Commons

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Capture, Concentration And Detection Of Salmonella In Foods Using Magnetic Ionic Liquids And Recombinase Polymerase Amplification, Stephanie A. Hice, Kevin D. Clark, Jared L. Anderson, Byron F. Brehm-Stecher 2018 Iowa State University

Capture, Concentration And Detection Of Salmonella In Foods Using Magnetic Ionic Liquids And Recombinase Polymerase Amplification, Stephanie A. Hice, Kevin D. Clark, Jared L. Anderson, Byron F. Brehm-Stecher

Food Science and Human Nutrition Publications

We have previously investigated the extraction and concentration of bacteria from model systems using magnetic ionic liquid (MIL) solvents, while retaining their viability. Here, we combine MIL-based sample preparation with isothermal amplification and detection of Salmonella-specific DNA using Recombinase Polymerase Amplification (RPA). After initial developmental work with Serratia marcescens in water, Salmonella Typhimurium ATCC 14028 was inoculated in water, 2% milk, almond milk or liquid egg samples and extracted using one of two MILs, including: trihexyl(tetradecyl)phosphonium cobalt(II) hexafluoroacetylacetonate ([P66614+][Co(hfacac)3–]) and trihexyl(tetradecyl)phosphonium nickel(II) hexafluoroacetylacetonate ([P66614+][Ni(hfacac)3–]). Viable cells were ...


Combination Regimens Using Dietary Components For The Chemoprevention Of Colorectal Cancer And Inflammation, Christina DiMarco-Crook 2018 University of Massachusetts Amherst

Combination Regimens Using Dietary Components For The Chemoprevention Of Colorectal Cancer And Inflammation, Christina Dimarco-Crook

Doctoral Dissertations

ABSTRACT

COMBINATION REGIMENS USING DIETARY COMPONENTS FOR THE

CHEMOPREVENTION OF COLORECTAL CANCER

AND INFLAMMATION

September 2018

CHRISTINA DIMARCO-CROOK, B.S., IMMACULATA UNIVERSITY

M.S., DREXEL UNIVERSITY

Ph.D., UNIVERSITY OF MASSACHUSETTS AMHERST

Directed by: Professor Hang Xiao

Dietary components have been found to effectively modulate multiple deregulated signaling pathways associated with the initiation and progression of carcinogenesis and inflammation in cellular and animal models. However, clinical studies have shown mixed results when examining the efficacy of individual dietary components, perhaps suggestive of the synergism that exists between multiple components within a particular food and the diet as a whole. Additional ...


Influences Of Antroquinonol And 4-Acetylantroquinonol B On Inflammatory Tumorigenesis In The Mcf-7 Breast Cancer Cell Line With Or Without Tnf-Α Stimulation, Ting-Chun Lin 2018 University of Massachusetts Amherst

Influences Of Antroquinonol And 4-Acetylantroquinonol B On Inflammatory Tumorigenesis In The Mcf-7 Breast Cancer Cell Line With Or Without Tnf-Α Stimulation, Ting-Chun Lin

Masters Theses

Breast cancer (BC) is one of the most common cancers among women worldwide that ~25% of new cancer cases diagnosed every year would be BC; moreover, ~15% of cancer deaths per year caused by BC makes it the leading cause of cancer death among women worldwide. To date, though the cause of a large proportion of BC are still unclear, recent studies have revealed that a supportive breast tissue microenvironment is critical for the development and progression of BC, especially the communication with immune cells within breast tissue. Therefore, breast inflammatory microenvironment is currently received a substantial attention in the ...


Comparison Of Maternal Beliefs About Causes Of Autism Spectrum Disorder And Association With Utilization Of Services And Treatments, Virginia Chaidez, Erik Fernandez y Garcia, Lulu W. Wang, Kathleen Angkustsiri, Paula Krakowiak, Irva Hertz-Picciotto, Robin L. Hansen 2018 University of Nebraska - Lincoln

Comparison Of Maternal Beliefs About Causes Of Autism Spectrum Disorder And Association With Utilization Of Services And Treatments, Virginia Chaidez, Erik Fernandez Y Garcia, Lulu W. Wang, Kathleen Angkustsiri, Paula Krakowiak, Irva Hertz-Picciotto, Robin L. Hansen

Nutrition and Health Sciences -- Faculty Publications

Background: This study aimed to describe parental perceptions of the causes of autism spectrum disorder (ASD) in an ethnically diverse sample and explore whether these perceptions relate to treatment choices.

Methods: The sample consisted of White (n=224), Hispanic (n=85) and Asian (n=21) mothers of a child with ASD. A mixed methods approach was used in this secondary analysis focusing on parental perceptions about the causes of ASD and the relationship of these to utilization of services and treatment.

Results: Environmental and genetic factors were most often believed to be the cause or one of the causes of ...


The Effect Of Wild Blueberry Bioactives On Endothelial Cell Migration And Angiogenesis: An In Vitro Mechanistic, Genomic And Proteomic Approach, Panagiotis Tsakiroglou 2018 University of Maine

The Effect Of Wild Blueberry Bioactives On Endothelial Cell Migration And Angiogenesis: An In Vitro Mechanistic, Genomic And Proteomic Approach, Panagiotis Tsakiroglou

Electronic Theses and Dissertations

The goal of this study is to investigate the effects of wild blueberry fractions (Anthocyanins and Phenolic acids) on vascular function and physiology. More specifically the potential effects of the above fractions and their combination in physiological concentrations on endothelial cell migration, angiogenesis, gene expression and proteins synthesis of markers related to the above processes. The objectives are to study whether anthocyanins, phenolic acids and their combinations (ACNs:PAs) affect: a) cell proliferation, b) speed of endothelial cell migration, c) angiogenesis, d) gene expression of genes critical for cell migration and angiogenesis such as RAC1, RHOA, AKT1, eNOS and VEGF ...


Nutritional Value And Flavor Of Turbot (Scophthalmus Maximus) Muscle As Affected By Cooking Methods, Xiu-Ping Dong, De-Yang Li, Ying Huang, Qiong Wu, Wen-Tao Liu, Lei Qin, Da-Yong Zhou, Sangeeta Prakash, Chenxu Yu 2018 Dalian Polytechnic University

Nutritional Value And Flavor Of Turbot (Scophthalmus Maximus) Muscle As Affected By Cooking Methods, Xiu-Ping Dong, De-Yang Li, Ying Huang, Qiong Wu, Wen-Tao Liu, Lei Qin, Da-Yong Zhou, Sangeeta Prakash, Chenxu Yu

Agricultural and Biosystems Engineering Publications

In this study, the effects of five cooking methods on nutritional value and flavor of turbot muscle were evaluated. Chemical compositions of samples were determined using AOAC methods, and the fatty-acid composition was determined by gas chromatography–mass spectrometry (GC–MS). Flavors of samples cooked using different methods were characterized by electronic nose. Volatile compounds were determined using solid-phase microextraction (SPME) followed by gas GC–MS. All the samples were investigated before/after cooking. The results showed that the fatty acids found in abundance in the raw sample were C22:6n-3, C16:0, C18:1n-7, and C20:5n-3. All of ...


Socio-Ecological Barriers To Dry Grain Pulse Consumption Among Low-Income Women: A Mixed Methods Approach, Shelly M. Palmer, Donna M. Winham, Ann M. Oberhauser, Ruth E. Litchfield 2018 Iowa State University

Socio-Ecological Barriers To Dry Grain Pulse Consumption Among Low-Income Women: A Mixed Methods Approach, Shelly M. Palmer, Donna M. Winham, Ann M. Oberhauser, Ruth E. Litchfield

Food Science and Human Nutrition Publications

The purpose of this study was to determine the socio-ecological influences on dry grain pulse consumption (beans, peas, lentils, chickpeas) among low-socioeconomic women in Iowa. Seven focus groups were conducted, with 36 women who qualified for income-based federal assistance. Data were collected from October 2017 to January 2018. Participants completed a survey that gathered individual demographics, assessed perceptions of dry grain pulses, and level of food security. Fifty-eight percent of the women were non-Hispanic white, and 39% were African American, all with an average age of 34.7 years. Thirty-three percent of the women consumed pulses less than once per ...


Extraction Of Phospholipids From A Dairy By-Product (Whey Protein Phospholipid Concentrate) Using Ethanol, Nathan R. Price, Tao Fei, Stephanie Clark, Tong Wang 2018 Iowa State University

Extraction Of Phospholipids From A Dairy By-Product (Whey Protein Phospholipid Concentrate) Using Ethanol, Nathan R. Price, Tao Fei, Stephanie Clark, Tong Wang

Food Science and Human Nutrition Publications

There has been a great interest in the phospholipids (PL) found in dairy products because of their health and functional properties. In this study, a technology that was originally developed for egg yolk PL extraction was applied to whey protein phospholipid concentrate (WPPC). This method successfully precipitated the proteins present in WPPC and extracted the lipids with a renewable alcoholic solvent, ethanol. The effect of ethanol concentration, extraction temperature, and extraction number on the recovery of total lipid, total PL, and individual PL class was evaluated. The optimum processing conditions for a combined 5-stage sequential extraction for producing a PL-enriched ...


Reporter Bacteriophage T7nlc Utilizes A Novel Nanoluc::Cbm Fusion For The Ultrasensitive Detection Of Escherichia Coli In Water., T.C. Hinkley, S. Garing, S. Singh, A-L. M. Le Ny, K.P. Nichols, J.E. Peters, Joey N. Talbert, S.R. Nugen 2018 Cornell University

Reporter Bacteriophage T7nlc Utilizes A Novel Nanoluc::Cbm Fusion For The Ultrasensitive Detection Of Escherichia Coli In Water., T.C. Hinkley, S. Garing, S. Singh, A-L. M. Le Ny, K.P. Nichols, J.E. Peters, Joey N. Talbert, S.R. Nugen

Food Science and Human Nutrition Publications

Rapid detection of bacteria responsible for foodborne diseases is a growing necessity for public health. Reporter bacteriophages (phages) are robust biorecognition elements uniquely suited for the rapid and sensitive detection of bacterial species. The advantages of phages include their host specificity, ability to distinguish viable and non-viable cells, low cost, and ease of genetic engineering. Upon infection with reporter phages, target bacteria express reporter enzymes encoded within the phage genome. In this study, the T7 coliphage was genetically engineered to express the newly developed luceriferase, NanoLuc (NLuc), as an indicator of bacterial contamination. While several genetic approaches were employed to ...


Epigenetic Modification Of Vitamin D-Induced Gene Expression In Human Colorectal And Breast Cancer Cell Lines, Sharmin Hossain 2018 University of Massachusetts Amherst

Epigenetic Modification Of Vitamin D-Induced Gene Expression In Human Colorectal And Breast Cancer Cell Lines, Sharmin Hossain

Doctoral Dissertations

Epigenetics is the study of the regulation of genes that is not dependent on nucleotide sequence. This may include heritable changes in gene activity and expression but also those that are not heritable. In recent years, epigenetic researchers have made great strides in understanding the molecular mechanisms behind cellular functions in response to diet, exercise, and other lifestyle modifications, like methylation, histone modification etc. It is now known that the epigenome is critical to healthy human development in addition to genetics, and dietary factors can modulate epigenetic alterations in cells. The classic view of cancer etiology is that genetic alterations ...


Impact Of Bodyweight On Tissue-Specific Folate Status, Genome Wide And Gene-Specific Dna Methylation In Normal Breast Tissues From Premenopausal Women, Armina-Lyn Frederick 2018 University of Massachusetts Amherst

Impact Of Bodyweight On Tissue-Specific Folate Status, Genome Wide And Gene-Specific Dna Methylation In Normal Breast Tissues From Premenopausal Women, Armina-Lyn Frederick

Masters Theses

Obesity has reached an epidemic level in the United States. A number of epidemiological studies have established obesity as a critical risk factor for postmenopausal breast cancer (post-BC), whereas a reverse association holds prior to menopause. A significant scientific gap exists in understanding the mechanism(s) underpinning this epidemiological phenomenon, particularly the reverse association between obesity and premenopausal breast cancer (pre-BC). This study aimed to understand how folate metabolism and DNA methylation informs the association between obesity and pre-BC. Fifty normal breast tissue samples were collected from premenopausal women who underwent reduction mammoplasty. We developed and measured the breast tissue ...


Transcription Factor 7-Like 2 (Tcf7l2) Gene Polymorphisms In Relation To The Risk Of Type 2 Diabetes In Three Ethnicities, Ling Xu 2018 Florida International University

Transcription Factor 7-Like 2 (Tcf7l2) Gene Polymorphisms In Relation To The Risk Of Type 2 Diabetes In Three Ethnicities, Ling Xu

FIU Electronic Theses and Dissertations

Type 2 Diabetes (T2D) disproportionally affects ethnic minorities in the United States. The development of T2D involves complex interaction between environmental factors and genetic predisposition. The genetic associations of six single nucleotide polymorphisms (SNPs) in TCF7L2 gene with the risk of T2D were evaluated in three high risk minority populations: Cuban Americans, Haitian Americans, and African Americans. For Cuban Americans, four SNPs (rs7901695, rs4506565, rs7903146 and rs11225537) were significantly associated with the risk of T2D after multivariable adjustment (p=0.018, p=0.016, p=0.014, and p=0.0008, respectively). Among controls, risk allele carriers of SNPs rs7901695 ...


Using A Model To Design Activity-Based Educational Experiences To Improve Cultural Competency Among Graduate Students, Yeon Bai, Kathleen D. Bauer 2018 Montclair State University

Using A Model To Design Activity-Based Educational Experiences To Improve Cultural Competency Among Graduate Students, Yeon Bai, Kathleen D. Bauer

Department of Nutrition and Food Studies Scholarship and Creative Works

To improve the cultural competency of 34 students participating in graduate nutrition counseling classes, the Campinha-Bacote Model of Cultural Competence in the Delivery of Health Care Services was used to design, implement, and evaluate counseling classes. Each assignment and activity addressed one or more of the five constructs of the model, i.e., knowledge, skill, desire, encounters, and awareness. A repeated measure ANOVA evaluated pre- and post-test cultural competence scores (Inventory for Assessing the Process of Cultural Competence among Healthcare Professionals). The overall cultural competence score significantly improved (p < 0.001) from “culturally aware” (68.7 at pre-test) to “culturally competent” (78.7 at post-test). Students significantly improved (p < 0.001) in four constructs of the model including awareness, knowledge, skill, and encounter. Factor analysis indicated that course activities accounted for 83.2% and course assignments accounted for 74.6% of the total variance of cultural competence. An activity-based counseling course encouraging self-evaluation and reflection and addressing Model constructs significantly improved the cultural competence of students. As class activities and assignments aligned well with the Campinha-Bacote Model constructs, the findings of this study can help guide health educators to design effective cultural competence training and education programs.


Biology Of Exosomes And Their Microrna Cargos In Human And Bovine Milk, Amy Lynn Leiferman 2018 University of Nebraska-Lincoln

Biology Of Exosomes And Their Microrna Cargos In Human And Bovine Milk, Amy Lynn Leiferman

Public Access Theses, Dissertations, and Student Research from the College of Education and Human Sciences

Exosomes are small, cargo-containing vesicles secreted by cells to facilitate intercellular communication. Of exosome cargos, microRNAs are especially interesting because of their involvement in gene regulation. Recently, our lab has shown that exosomes and their microRNA cargo are absorbed through the diet and elicit effects exogenously. Human and animal milk contain exosomes, which may have implications in infant and adult nutrition. There is evidence that bovine milk exosomes enhance growth of murine C2C12 myotube cell cultures, but whether this translates to muscle in vivo is unknown. The USDA National Nutrient Database for Standard Reference is lacking up-to-date information about human ...


Radical Social Ecology As Deep Pragmatism: A Call To The Abolition Of Systemic Dissonance And The Minimization Of Entropic Chaos, Arielle Brender 2018 Fordham University

Radical Social Ecology As Deep Pragmatism: A Call To The Abolition Of Systemic Dissonance And The Minimization Of Entropic Chaos, Arielle Brender

Student Theses 2015-Present

This paper aims to shed light on the dissonance caused by the superimposition of Dominant Human Systems on Natural Systems. I highlight the synthetic nature of Dominant Human Systems as egoic and linguistic phenomenon manufactured by a mere portion of the human population, which renders them inherently oppressive unto peoples and landscapes whose wisdom were barred from the design process. In pursuing a radical pragmatic approach to mending the simultaneous oppression and destruction of the human being and the earth, I highlight the necessity of minimizing entropic chaos caused by excess energy expenditure, an essential feature of systems that aim ...


Session 3 Discussion: The Microstructure Of Eating, Cordelia Running, Bryony James, James Hollis, Kelly Higgins 2018 Purdue University

Session 3 Discussion: The Microstructure Of Eating, Cordelia Running, Bryony James, James Hollis, Kelly Higgins

Food Science and Human Nutrition Publications

The Microstructure of Eating.


Antiproliferative Activity Of Carotenoids, Phenolic Compounds, And Volatile Terpenoids In Carrots (Daucus Carota L.), Brittany Frederick 2018 University of Arkansas, Fayetteville

Antiproliferative Activity Of Carotenoids, Phenolic Compounds, And Volatile Terpenoids In Carrots (Daucus Carota L.), Brittany Frederick

Theses and Dissertations

Epidemiological studies have shown an association between high carrot consumption and low prevalence of cancer. This observation has been thought to be attributed to carrot carotenoids. Despite this, various intervention trials have displayed no changes in incidence or increased incidence of cancer with carotenoid supplementation. It is possible that carrot phenolics are responsible for this association, though this has not been widely accepted. Volatile terpenoids from carrots have not been studied in this regard. Therefore, the primary objective of this study was to compare the antiproliferative effects of carotenoids, phenolics, and volatile terpenoids extracted from carrots on Caco-2 colon cancer ...


Effects Of Mitochondrial Nadp+-Dependent Isocitrate Dehydrogenase Deficiency On Fructose-Induced Obesity In Mice, Allison Michelle Montalbano, Kaleigh Elizabeth Beane 2018 University of Arkansas, Fayetteville

Effects Of Mitochondrial Nadp+-Dependent Isocitrate Dehydrogenase Deficiency On Fructose-Induced Obesity In Mice, Allison Michelle Montalbano, Kaleigh Elizabeth Beane

Human Nutrition and Hospitality Management Undergraduate Honors Theses

Obesity prevalence in the United States continues to increase and is associated with health consequences such as type 2 diabetes, hypertension, atherosclerosis, and hyperlipidemia. Among many contributing factors to obesity, fructose may be one of the major reasons as it disrupts the antioxidant system thereby resulting in an accumulation of reactive oxidative species and leading to obese conditions. The enzyme, isocitrate dehydrogenase 2 (IDH2), reduces nicotinamide adenine dinucleotide phosphate from the TCA Cycle, hence might be implicated with not only energy metabolism but also cellular redox homeostasis. Therefore, the hypothesis was that IDH2 deficiency in mice would exacerbate hepatic lipid ...


The Effects Of Inulin And Galactooligosaccharides On The Production Of Reuterin By Lactobacillus Reuteri, Micah Forshee 2018 Olivet Nazarene University

The Effects Of Inulin And Galactooligosaccharides On The Production Of Reuterin By Lactobacillus Reuteri, Micah Forshee

Honors Program Projects

The microbiome is a dynamic community that can positively and negatively influence host health. Lactobacillus reuteri is a probiotic that has received much attention for its ability to inhibit pathogens such as Salmonella Typhimurium, Escherichia coli, and Clostridium difficile. It does so by its unique ability to metabolize glycerol into the antimicrobial compound 3-HPA, which is commonly referred to as reuterin. The ability to secrete reuterin is dependent not only on glycerol availability but also the concentration of glucose. In fact, there appears to be a “goldilocks” ratio between glucose and glycerol as either too much or too little glucose ...


Pkm2 Influences The Metabolic Fate Of Butyrate In Colorectal Cancer Cells, Megan Louise Pence 2018 University of Tennessee, Knoxville

Pkm2 Influences The Metabolic Fate Of Butyrate In Colorectal Cancer Cells, Megan Louise Pence

Chancellor’s Honors Program Projects

No abstract provided.


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