The Ecosystem Of A Remarkable Glaciovolcanic Cave At Mount Meager, British Columbia, Canada,
2024
Alberta Speleological Society
The Ecosystem Of A Remarkable Glaciovolcanic Cave At Mount Meager, British Columbia, Canada, Christian Stenner, Kathleen Graham, Morgan Cable, Glyn Williams-Jones, Jared J. Clance, Jacob Shaffer, Jill Mikucki, Anna Szynkiewicz, Olivia Vinnes, Michael Paton, Jeremy Nash
FRONTIERS UNBOUND: Exploring Extreme Environments
No abstract provided.
Cave Ice Microbiome – Reservoir For New Biomolecules In Medicine And Biotechnologies,
2024
Institute of Biology Bucharest of the Romanian Academy
Cave Ice Microbiome – Reservoir For New Biomolecules In Medicine And Biotechnologies, Victoria Ioana Paun, Corina Itcus, Carmen Mariana Chifiriuc, Paris Lavin, Cristina Purcarea
FRONTIERS UNBOUND: Exploring Extreme Environments
No abstract provided.
Antarctic Glacier Melting Modeling Distribution Of Bacterial Community Structure,
2024
Institute of Biology Bucharest of the Romanian Academy, Bucharest, Romania
Antarctic Glacier Melting Modeling Distribution Of Bacterial Community Structure, Corina Itcus, Constantin Marin, Soon Gyu Hong, Manuela Elisabeta Sidoroff, Cristian Coman, Cristina Purcarea
FRONTIERS UNBOUND: Exploring Extreme Environments
No abstract provided.
Antibiotic-Resistant Gram-Negative Bacteria In A Local Wastewater Treatment Plant In North Georgia,
2024
University of North Georgia
Antibiotic-Resistant Gram-Negative Bacteria In A Local Wastewater Treatment Plant In North Georgia, Nou Moua, Lindsay Mccuen, Sarah White, Swapna Bhat, Margi Flood, Jeanelle Morgan
Georgia Journal of Science
Antibiotics are some of the most essential lifesaving drugs currently available to us against infectious diseases. Several pathogens are developing resistance to these drugs and many have become resistant to multiple antibiotics. Such multi-drug resistance is becoming a global issue and has been found in many environments including wastewater treatment plants (WWTPs). In this study, we sampled a local WWTP for antibiotic-resistant bacteria. This WWTP is in North Georgia and runs directly into Lake Lanier, which is the main source of drinking water for the Atlanta area as well as a popular spot for water recreation. We obtained treated and …
Multidrug-Resistant Esbl-Producing Escherichia Coli Coexisting With Colistin-Resistance Genes In Pig Farms, Central Thailand,
2024
Chulalongkorn University, College of Public Health Science
Multidrug-Resistant Esbl-Producing Escherichia Coli Coexisting With Colistin-Resistance Genes In Pig Farms, Central Thailand, Wipawee Songsaeng, Nutthee Am-In, Nuvee Prapasarakul, Wandee Sirichokchatchawan
The Thai Journal of Veterinary Medicine
The presence of extended-spectrum beta-lactamase and colistin resistance E. coli in food-producing animals is a public health concern. This study was conducted using a rigorous methodology to survey the prevalence of ESBL-producing and colistin-resistant E. coli from pig farms in the central part of Thailand. A total of 519 samples were collected from fecal, feed, and waste in 53 farms located in central Thailand. All samples were inoculated onto selective agar, and species identification was performed using biochemical assays and MAlDI-TOF MS. Antimicrobial susceptibility was determined by broth microdilution, and ESBL production was confirmed by the combination disc diffusion method. …
Time-Transgressive Microbial Diversity In A Tropical Bat Guano Accumulation, Deer Cave, Mulu, Borneo,
2024
Carleton University, Canada
Time-Transgressive Microbial Diversity In A Tropical Bat Guano Accumulation, Deer Cave, Mulu, Borneo, Joyce Lundberg, Donald A. Mcfarlane
International Journal of Speleology
Bat guano accumulations in caves can constitute large deposits of organic material, particularly chitin and its degradation products, that host a diverse microbiome. Previous microbiological studies have not addressed the stratified nature of these deposits in the context of the timeframe of the decomposition process. We present such data (temperature, pH, DNA sampling of eight carbon-dated strata) for a 75 cm-deep tropical guano accumulation that undergoes complete decomposition over a timescale of ~120 years. The aerobic, surface layer of freshly-fallen guano has the greatest diversity of taxa, with Bacteroidota co-dominant with Pseudomonadota, and includes many taxa unique to this …
Investigating The Presence Of Bacterial Phytoplasma Disease In Grasses Of Central Texas And Their Associated Hemiptera,
2024
Texas A&M University-San Antonio
Investigating The Presence Of Bacterial Phytoplasma Disease In Grasses Of Central Texas And Their Associated Hemiptera, Danielle Rios
Masters Theses (Archived)
No abstract provided.
Investigation Of The Microbiome Of A Recirculating Aquaculture System (Ras) For Crassostrea Virginica Larvae,
2024
The University of Southern Mississippi
Investigation Of The Microbiome Of A Recirculating Aquaculture System (Ras) For Crassostrea Virginica Larvae, Isabelle Townsend
Master's Theses
Recirculating Aquaculture Systems (RAS) offer control over physicochemical and biological factors impacting aquaculture success. One understudied facet of RASs is the water microbiome. Oyster larval microbiomes are shaped by their aquatic microbiomes. Understanding the dynamics of and the factors shaping RAS microbiome may provide insights to how the microbiome can be a tool for optimizing larval production. This study’s goals were to investigate the microbiome stability of a RAS rearing oyster larvae across time and space, determine impacts of larval presence on the microbiome, and examine connections between larvae production outcomes and microbiome stability. Water samples were collected weekly from …
Assessment Of Tce And Chiral Pcb Dechlorination Rate, Congener Diversity, And Enantioselectivity In Town Creek, Sc, Usa Sediment Microcosms,
2024
Clemson University
Assessment Of Tce And Chiral Pcb Dechlorination Rate, Congener Diversity, And Enantioselectivity In Town Creek, Sc, Usa Sediment Microcosms, Catherine P. Sumner
All Dissertations
Polychlorinated biphenyls (PCBs) and trichloroethene (TCE) are ubiquitous contaminants and are recognized as persistent organic pollutants due to their extreme chemical stability. PCBs were manufactured by chlorinating biphenyls that created 209 congeners with various structures, of which 19 are chiral and can exist as a pair of stable atropisomers. PCBs have been known to cause developmental and neurological toxicity in humans and wildlife; they can act as endocrine disrupters, carcinogens, and teratogens. Sangamo Weston Inc. was an industrial plant located near Town Creek in Pickens Country, South Carolina, that manufactured capacitors and used Aroclors 1016 and 1254 as dialectic fluids …
Effects Of Spatial And Temporal Variability On Bacterial Diversity And Community Composition In Roof-Harvested Rainwater Across Central Virginia,
2024
Longwood University
Effects Of Spatial And Temporal Variability On Bacterial Diversity And Community Composition In Roof-Harvested Rainwater Across Central Virginia, Jade Riddle
Longwood Senior Thesis Proposal
No abstract provided.
Characterizing The Quorum Sensing (Qs) System In The Model Eukaryote Chlamydomonas Reinhardtii,
2024
Florida Institute of Technology
Characterizing The Quorum Sensing (Qs) System In The Model Eukaryote Chlamydomonas Reinhardtii, Kirstin Donnette Cutshaw
Theses and Dissertations
Group behaviors in microorganisms are often regulated by low-molecular weight molecules that enable them to sense population density, a signaling system known as quorum sensing (QS). QS in prokaryotes has provided insight into the role ‘social’ behaviors play in the bacterial world and refined our models of virulence, bacterial motility, and more. While less common, similar behaviors are observed in certain fungi, primarily yeasts, in which QS controls cell morphology, biofilm production, and other phenotypes. We have recently pursued the potential for QS to be more broadly distributed among unicellular eukaryotes, which could significantly alter our understanding of social behaviors …
Optimizing The Expression Of Polyethylene Terephtalate Hydrolase-Encoding Synthetic Gene In Escherichia Coli Arctic Express (De3),
2024
Department of Biology, University of Indonesia, Depok 16424, West Java
Optimizing The Expression Of Polyethylene Terephtalate Hydrolase-Encoding Synthetic Gene In Escherichia Coli Arctic Express (De3), Jocelyn Nataniel, Maria Ulfah, Dini Achnafani, Niknik Nurhayati, Gabriela Christy Sabbathini, Sri Rezeki Wulandari, Abinawanto Abinawanto, Is Helianti
Makara Journal of Science
The waste of polyethylene terephthalate (PET) plastic waste in Indonesia is a pressing concern due to its slow degradation and potential environmental damage. One promising solution is to utilize polyethylene terephthalate hydrolase from Ideonella sakaiensis (IsPETase), an enzyme that specifically degrades PET. However, inducing the expression of IsPETase synthetic gene in Escherichia coli BL21 (DE3) has been challenging because much of it remains insoluble. This study aimed to express IsPETase in E. coli Arctic Express (DE3) and optimize the conditions to enhance its production. First, pET22b(+)pelB-IsPETase was inserted into E. coli Arctic Express (DE3). The …
Metagenomic Analysis Of Antarctic Microbial Communities,
2024
University of New Mexico
Metagenomic Analysis Of Antarctic Microbial Communities, David Michael Robinson
Biology ETDs
The research presented in this dissertation focuses on microbial roles in biogeochemical cycling in Antarctic aquatic environments. The major objective of my research was to examine the impact of biotic and abiotic pressures on nutrient cycling in microbially dominated systems. I used three perennially ice covered lakes in the McMurdo Dry Valleys (MDVs) along with subglacial lakes Whillans and Mercer as natural case studies. The MDVs are located in Victoria Land, East Antarctica and have been studied since 1993 as part of the McMurdo Long Term Ecological Research Project. Viruses in the MDVs were shown to be seasonally abundant and …
Degradation Of Chlorpyrifos Residue On Tropical Inceptisol Soil With Indigenous Bacteria Treatment,
2024
Research Center for Horticulture and Estate Crops, National Research and Innovation Agency, Cibinong Science Center, Bogor 16915, Indonesia
Degradation Of Chlorpyrifos Residue On Tropical Inceptisol Soil With Indigenous Bacteria Treatment, Asep Kurnia, Hidayatuz Zu`Amah, Baiq Nunung Sulastri, Ria Fauriah Makmur
Makara Journal of Science
The use of soil microbes is one of the most effective methods for degrading pesticide residue in soil. Microbes can accelerate the degradation of persistent pollutants including chlorpyrifos in soil. This research aims to find soil bacteria with the greatest potential to degrade chlorpyrifos residue in tropical inceptisol soil and determine its degradation index (DI). The soil used in this experiment was collected from agricultural fields in the districts of Demak, Central Java Province, Indonesia. The experiment was performed using factorial randomize complete block design composed of two factors and four replications. The first factors were made up of two …
Prochlorococcus Death By A Ubiquitous Zooplankton Filter Feeder,
2024
Portland State University
Prochlorococcus Death By A Ubiquitous Zooplankton Filter Feeder, Avery Harman
University Honors Theses
Prochlorococcus is a globally abundant cyanobacterium that can help reveal the ecology of microorganisms in the ocean. Specifically, mortality by zooplankton such as appendicularians is globally important and Prochlorococcus can help reveal some of the choices made around feeding for this ubiquitous filter feeder. Two ecotypes of Prochlorococcus were introduced to appendicularians to determine if appendicularian feed selectively between these ecotypes. Further, a single ecotype of Prochlorococcus was fed to appendicularians over a variety of life stages to determine if retention rate changed with appendicularian life stage. My results reveal that there is no significant selection between the two Prochlorococcus …
Heavy Metals Implications To Sediment Microbiome And Coral Response To Arsenic Dosing,
2024
Nova Southeastern University
Heavy Metals Implications To Sediment Microbiome And Coral Response To Arsenic Dosing, Dimitrios G. Giarikos, Amy Hirons, Jose V. Lopez, Abigail Renegar, Jason Gershman
SECLER Data
No abstract provided.
Establishment Of Bacteroides In The Gut And Its Role In The Cystic Fibrosis Gut-Lung Axis,
2024
Dartmouth College
Establishment Of Bacteroides In The Gut And Its Role In The Cystic Fibrosis Gut-Lung Axis, Rebecca A. Valls
Dartmouth College Ph.D Dissertations
In the quest to understand the complex interplay between the gut microbiota and cystic fibrosis (CF), a disease characterized by chronic upper respiratory complications and an exacerbated proinflammatory response, our lab engaged in a collaborative study with Dartmouth-Hitchcock Medical Center (DHMC) to characterize the gut microbiota of pediatric CF patients. Initial analyses established a connection between gut microbial composition and respiratory health, particularly highlighting a scarcity of Bacteroides species compared to healthy controls. In following investigations, we utilized random forest models to understand how stool microbiota may influence clinical outcomes of these pediatric CF patients, including: age, upper respiratory infections, …
Characterization Of A Novel Metalloid Reductase Outer Membrane Porin Rara From Sulfurospirillum Barnesii Ses-3,
2024
Duquesne University
Characterization Of A Novel Metalloid Reductase Outer Membrane Porin Rara From Sulfurospirillum Barnesii Ses-3, Narthana Jeganathar Kanmanii
Electronic Theses and Dissertations
Sulfurospirillum barnesii SES-3 is a gram-negative epsilon proteobacterium that grows anaerobically by coupling the oxidation of lactate to the reduction of arsenate. Previous work on metal and metalloid metabolism in S. barnesii had identified a redox active protein, designated RarA that when provided an artificial electron donor (i.e., reduced methyl viologen, FMN, or NADH), could reduce a variety of substrates, including arsenate, selenate, selenite, nitrite, and even phosphate. The enzyme’s inferred amino acid sequence showed weak similarity (30%) to outer membrane porins with homologs found in only certain species of Sulfurospirillum. Further, the enzyme did not contain cysteine residues …
The Influence Of A Ubiquitous Filter Feeder On Coastal Microbial Communities.,
2024
Portland State University
The Influence Of A Ubiquitous Filter Feeder On Coastal Microbial Communities., Melissa Steinman, Moritz S. Schmid, Robert K. Cowen, Su Sponaugle, Kelly R. Sutherland, Anne W. Thompson
Student Research Symposium
Doliolids have a unique ability to impact the marine microbial community through bloom events and high filtration rates. Their predation on large eukaryotic microorganisms is established and evidence of predation on smaller prokaryotic microorganisms is beginning to emerge. We studied the retention of both eukaryotic and prokaryotic microbial taxa by wild-caught doliolids in the northern California Current system. We use qPCR to quantify the impact of doliolids on three important and globally abundant taxa: Synechococcus, SAR11 and diatoms. Doliolids were collected during bloom events identified at three different shelf locations with variable upwelling intensities. We discovered that in addition …
Fiddler Crab Gut Microbiomes As An Indicator For Eutrophication Due To Waste-Water Contamination In Estuaries,
2024
Coastal Carolina University
Fiddler Crab Gut Microbiomes As An Indicator For Eutrophication Due To Waste-Water Contamination In Estuaries, Madison L. Geraci
Honors Theses
Estuaries are unique biogeochemical zones in coastal systems that provide a myriad of ecological functions: providing habitat for critically important fishery species, diverse species of plants and animals, and contributing to nutrient cycling. Estuaries are vulnerable to anthropogenic stressors as they are intersections between marine and terrestrial communities and are often the hotspot of coastal development and activity. Estuarine sediments act as repositories for anthropogenic contaminants, including excess nutrient runoffs and waste-water pollution that can cause states of eutrophication. Microorganisms are sensitive to these environmental changes, presenting a unique opportunity of being useful bioindicators of environmental stress. Isolating microorganisms from …
