Parasitology Commons^™

Microsporidia Spore Adherence And Host Cell Infection In Vitro, Cory A. Leonard

Electronic Theses and Dissertations

Microsporidia infect invertebrate and vertebrate animals. Human pathogenic microsporidia are associated with severe disease in immunocompromised individuals, and mostly asymptomatic infection in the immunocompetent. Treatment options for microsporidiosis are limited, incompletely effective, and associated with toxicity. Furthermore, microsporidia infection of healthy individuals is poorly understood, and the consequences of asymptomatic infection have not been determined. Little is known about the molecular mechanisms of microsporidia infection, but such information is essential for the development of new therapies. Spores adhere to host cell surfaces in vitro. Our laboratory has focused on determining specific host cell and microsporidia spore surface participants in spore …

Coccidia Of Gerbils From Mongolia, Ethan Thomas Jensen

School of Biological Sciences: Dissertations, Theses, and Student Research

In this study, gerbils collected in the Mongolia over the summers of 2009-2012 were examined for coccidia. In total, 171 gerbils of three species from 22 localities were examined for coccidia. Coccidian oocysts were identified from 21 gerbils, but those found in 1 of those gerbils were probably pseudoparasites of the host from which they were recovered. From the remaining 20 gerbils, 7 morphotypes of Eimeria and 1 morphotype of Isospora were identified. Four of the 7 morphotypes of Eimeria were attributed to new species which were described in this study. In addition, 10 previously described species of Eimeria were …

Spatially Explicit Modeling Of Schistosomiasis Risk In Eastern China Based On A Synthesis Of Epidemiological, Environmental, And Intermediate Host Genetic Data, Matthias Schrader, Torsten Hauffe, Zhijie Zhang, George M. Davis, Fred Jopp, Justin V. Remais, Thomas Wilke

Microbiology, Immunology, and Tropical Medicine Faculty Publications

Schistosomiasis japonica is a major parasitic disease threatening millions of people in China. Though overall prevalence was greatly reduced during the second half of the past century, continued persistence in some areas and cases of re-emergence in others remain major concerns. As many regions in China are approaching disease elimination, obtaining quantitative data on Schistosoma japonicum parasites is increasingly difficult. This study examines the distribution of schistosomiasis in eastern China, taking advantage of the fact that the single intermediate host serves as a major transmission bottleneck. Epidemiological, population-genetic and high-resolution ecological data are combined to construct a predictive model capable …

Ectoparasites Of The Virginia Opossum (Didelphis Virginiana), Raccoon (Procyon Lotor), And Striped Skunk (Mephitis Mephitis) From Keith County, Nebraska, Lance A. Durden, Dennis J. Richardson

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Six Virginia opossums (Didelphis virginiana), nine raccoons (Procyon lotor) and one striped skunk (Mephitis mephitis) collected from Keith County Nebraska were examined for ectoparasites. All three host species were parasitized by adults of the American dog tick, Dermacentor variabilis. Opossums were also parasitized by the flea Pulex simulans and the tiny fur mite Didelphilichus serri­fer; the latter species represents a new state record for Nebraska. Raccoons were also parasitized by P. simulans and by the lago­morph-associated flea Euhoplopsyllus glacialis affinis, whereas the skunk was also parasitized by the chewing louse Neotrichodectes …

Estudios Científicos En El Estado De Hidalgo Y Zonas Aledañas, Volumen Ii, Griselda Pulido-Flores, Scott Monks

Zea E-Books Collection

Los 16 capítulos que conforman este libro son una contribución al conocimiento de la biodiversidad y conservación de los recursos naturales en el estado de Hidalgo, México.

Se aborda el derecho de propiedad intelectual en la biodiversidad, citando algunos ejemplos de México. Así mismo, se presenta un estudio de la biodiversidad y distribución de la herpetofauna en cuatro tipos de vegetación. Desde el punto de vista de la helmintología se presentan dos estudios de los helmintos parásitos de vertebrados silvestres; con nuevos registros de hospederos y localidades de algunas especies de helmintos para Hidalgo. También, se aborda el uso de …

Microproteinuria During Opisthorchis Viverrini Infection: A Biomarker For Advanced Renal And Hepatobiliary Pathologies From Chronic Opisthorchiasis, Prasert Saichua, Paiboon Sithithaworn, Amar R. Jariwala, David J. Diemert, Jiraporn Sithithaworn, Banchob Sripa, Thewarach Laha, Eimorn Mairiang, Chawalit Pairojkul, Maria V. Periago, Narong Khuntikeo, Jason Mulvenna, Jeffrey M. Bethony

Microbiology, Immunology, and Tropical Medicine Faculty Publications

Approximately 680 million people are at risk of infection with Opisthorchis viverrini (OV) andClonorchis sinensis, with an estimated 10 million infected with OV in Southeast Asia alone. While opisthorchiasis is associated with hepatobiliary pathologies, such as advanced periductal fibrosis (APF) and cholangiocarcinoma (CCA), animal models of OV infection show that immune-complex glomerulonephritis is an important renal pathology that develops simultaneously with hepatobiliary pathologies. A cardinal sign of immune-complex glomerulonephritis is the urinary excretion of immunoglobulin G (IgG) (microproteinuria). In community-based studies in OV endemic areas along the Chi River in northeastern Thailand, we observed that over half of …

Efficacies Of Fenbendazole And Albendazole In The Treatment Of Commercial Turkeys Artificially Infected With Ascaridiadissimilis, Jessica Perkins

Animal Science Undergraduate Honors Theses

The goal of this research was to determine the extent of anthelmintic resistance that turkey roundworms, Ascaridiadissimilis, have developed to anti-parasitic chemicals used in commercial turkey operations. Roundworm infections in turkeys have resulted in monetary losses for the poultry industry for years, generally due to poor feed conversion. The infection itself is generally subclinical and many turkeys have a light to moderate worm burden. Since parasitisms are light, this leads to the infections being noticed only during processing.A. dissimilis infections consist of adult worms and developing larvae with the latter comprising most of the worm burden and causing the most …

American Society Of Parasitologists Newsletter, V. 35, No. 1, Spring 2013, Scott Lyell Gardner

American Society of Parasitologists: Newsletter

American Society of Parasitologists Newsletter, volume 35, number 1, Spring 2013

Molecular Analysis Of The Localization And Function Of Caspase-Like Proteins In Dictyostelium Discoideum And The Parasitic Protozoan Acanthamoeba Castellanii, Entsar Jabbar Saheb Kano

Theses and Dissertations

Two novel families of caspase-like proteins, metacaspases and paracaspases, have been discovered but have remained poorly characterized. Metacaspase type-1 (Acmcp) has been identified in Acanthamoeba castellanii , which is an opportunistic protozoan parasite that causes several diseases in humans. On the other hand, paracaspase (Ddpcp) has been found in the protozoan Dictyostelium discoideum . D. discoideum is well-researched model organism that is appropriate for studying these caspase-like proteins. In this study, we investigated the localization and molecular function of metacaspase and paracaspase proteins by over-expressing them in D. discoideum . Separate cell lines over-expressing a GFP-tagged version of the full …

Break Out: Urogenital Schistosomiasis And Schistosoma Haematobium Infection In The Post-Genomic Era, Paul J. Brindley, Peter J. Hotez

Microbiology, Immunology, and Tropical Medicine Faculty Publications

No abstract provided.

Review Of Haploporid (Trematoda) Genera With Ornate Muscularization In The Region Of The Oral Sucker, Including Four New Species And A New Genus, Eric E. Pulis, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Species of the Haploporidae Nicoll, 1914 with elaborate muscularization of the oral sucker belong in three trematode genera, including three new species and a new genus from the intestine of fishes in Australian waters. Spiritestis Nagaty, 1948 is resurrected and S. herveyensis n. sp. is described from the mullet Moolgarda seheli (Forsskål) collected in Hervey Bay, Queensland, Australia; the latter differs from S. arabii Nagaty, 1948 in that the position of the genital pore is pharyngeal rather than post-pharyngeal and the geographical range is off Australia rather than the Red Sea. A new genus is proposed for two new species, …

Effects Of Treatment On Ige Responses Against Parasite Allergen-Like Proteins And Immunit To Reinfection In Childhood Schistosome And Hookworm Coinfections, Angela Pinot De Moira, Frances M. Jones, Shona Wilson, Edridah Tukahebwa, Colin M. Fitzsimmons, Joseph K. Mwatha, Jeffrey M. Bethony, Narcis B. Kabatereine, David W. Dunne

Microbiology, Immunology, and Tropical Medicine Faculty Publications

Naturally occurring human immunity to both schistosomiasis and hookworm infection has been associated with IgE responses against parasite allergen-like proteins. Since the two helminths frequently coinfect the same individuals, there is growing advocacy for their concurrent treatment. However, both helminths are known to exert strong immunomodulatory effects; therefore, coinfected individuals could have immune responses different from those characteristically seen in monoinfected individuals. In this study, we measured changes in IgE, IgG1, and IgG4 responses to schistosome and hookworm antigens, including the allergen-like proteins Schistosoma mansoni tegumental-allergen-like 1 protein (SmTAL1), SmTAL2, and Necator americanus Ancylostoma-secreted protein-2 (Na-ASP-2), following concurrent …

Transcriptional Responses Of In Vivo Praziquantel Exposure In Schistosomes Identifies A Functional Role For Calcium Signalling Pathway Member Camkii, Hong You, Donald P. Mcmanus, Wei Hu, Michael J. Smout, Paul J. Brindley, Geoffrey N. Gobert

Microbiology, Immunology, and Tropical Medicine Faculty Publications

Treatment for clinical schistosomiasis has relied centrally on the broad spectrum anthelmintic praziquantel; however, there is limited information on its mode of action or the molecular response of the parasite. This paper presents a transcriptional and functional approach to defining the molecular responses of schistosomes to praziquantel. Differential gene expression in Schistosoma japonicum was investigated by transcriptome-wide microarray analysis of adult worms perfused from infected mice after 0.5 to 24 hours after oral administration of sub-lethal doses of praziquantel. Genes up-regulated initially in male parasites were associated with “Tegument/Muscle Repair” and “Lipid/Ion Regulation” functions and were followed by “Drug Resistance” …

Patent Landscape Of Helminth Vaccines And Related Technologies, Jon R. Cavicchi, Stanley P. Kowalski, John Schroeder, Rayna Burke, Jillian Michaud-King

Law Faculty Scholarship

Executive Summary This report focuses on patent landscape analysis of technologies related to vaccines targeting parasitic worms, also known as helminths. These technologies include methods of formulating vaccines, methods of producing of subunits, the composition of complete vaccines, and other technologies that have the potential to aid in a global response to this pathogen. The purpose of this patent landscape study was to search, identify, and categorize patent documents that are relevant to the development of vaccines that can efficiently promote the development of protective immunity against helminths. The search strategy used keywords which the team felt would be general …

Title Page, Contents, Contributors For Estudios Científicos En El Estado De Hidalgo Y Zonas Aledañas, Volumen Ii, Griselda Pulido-Flores, Scott Monks

Estudios científicos en el estado de Hidalgo y zonas aledañas

Title Page

Contents

Contributors

Los Parásitos Y El Estudio De Su Biodiversidad: Un Enfoque Sobre Los Estimadores De La Riqueza De Especies, Christian E. Bautista-Hernández, Scott Monks, Griselda Pulido-Flores

Estudios científicos en el estado de Hidalgo y zonas aledañas

Durante años los parásitos fueron considerados únicamente como agentes patógenos que debían ser eliminados, sin considerar que son un componente importante de la biodiversidad, que son capaces de aportar información de sí mismos y de otros organismos. Existen estimadores de riqueza que predicen el número de especies para una comunidad a partir de la riqueza observada y de la abundancia de las especies o datos de incidencia; pueden ser estimadores paramétricos y no paramétricos. La comparación de la riqueza de especies entre componente de comunidades de parásitos frecuentemente pueden ser difícil de evaluar debido a que los datos no se …

El Uso De Helmintos Parásitos Como Bioindicadores En La Evaluación De La Calidad Del Agua: Lago De Tecocomulco Vs. Laguna De Metztitlán, Hidalgo, México, Scott Monks, Griselda Pulido-Flores, Christian E. Bautista-Hernández, Berenice Alemán-García, Jorge Falcón-Ordaz, Juan Carlos Gaytán-Oyarzún

Estudios científicos en el estado de Hidalgo y zonas aledañas

Como parte integral de la evaluación de la calidad ambiental del estado de Hidalgo, este estudio tiene énfasis en los helmintos parásitos de los peces y su uso como bioindicadores de la calidad de agua en Lago de Tecocomulco y Laguna de Metztitlán. Como parte del estudio, se obtuvieron los parámetros ecológicos de la infección para cada especie. En Lago Tecocomulco se registraron dos especies de digéneos (larvas), Posthodiplostomum sp. y Diplostomum sp. en el cerebro, los ojos y el mesenterio de dos especies de peces nativos de la zona (Chirostoma jordani y Girardinicthys viviparus); un céstodo (larva), …

Helmintos Parásitos De Algunos Roedores (Mammalia: Rodentia) En San Miguel De Allende, Tepeapulco, Hidalgo, México, Griselda Pulido-Flores, Scott Monks, Jorge Falcón-Ordaz

Estudios científicos en el estado de Hidalgo y zonas aledañas

Los helmintos son un componente de la biodiversidad, que proveen información sobre la historia natural de los hospederos, y de las relaciones tróficas. El conocimiento de la diversidad de roedores y sus parásitos en Hidalgo es escaso y puntual. Por la amplia distribución de los roedores, su adaptabilidad a los ambientes modificados y por el riesgo zoonótico que representan, es importante conocer la parasitofauna de estos hospederos. El objetivo del este trabajo fue registrar la helmintofauna de algunos roedores en San Miguel de Allende, Tepeapulco, Hidalgo, México. Entre agosto de 2005 y junio de 2007 se colectaron 19 roedores (12 …

Análisis Morfométrico De Glypthelmins Quieta (Stafford, 1900) Stafford, 1905 (Digenea: Macroderoididae) En La Reserva De La Biosfera Barranca De Metztitlán, Hidalgo, México, Francisco Alemán-Muñoz, Griselda Pulido-Flores, Scott Monks, Jorge Falcón-Ordaz

Estudios científicos en el estado de Hidalgo y zonas aledañas

Se evaluó la variación morfológica de 30 ejemplares adultos de la especie Glypthelmins quieta, parásitos de Lithobates spectabilis en localidad de Metznoxtla, en la Reserva de la biosfera Barranca de Metztitlán, Hidalgo. El objetivo del presente trabajo fue contribuir al conocimiento de la variación morfológica de G. quieta, y evaluar si los caracteres de presencia ausencia de glándulas perifaringeas y forma de las glándulas mediales son validos para la asignación de ejemplares a una especie. Se generó una base de datos morfológicos realizando tres análisis de componentes principales (ACP). El primer ACP se realizó con la totalidad de …

Análisis De Metales Pesados En Un Céstodo De La Familia Dilepididae, Jorge Falcón-Ordaz, Scott Monks, Griselda Pulido-Flores, Claudia Romo-Gómez

Estudios científicos en el estado de Hidalgo y zonas aledañas

La concentración de plomo (Pb), cadmio (Cd), zinc (Zn), níquel (Ni), hierro (Fe) y aluminio (Al) fue determinada en un céstodo de la familia Dilepididae, parásito de Peromyscus difficilis colectados en una zona rural del Estado de México; con respecto a trabajos previos se obtuvieron valores altos de concentración para Pb y Zn mientras que para Ni sus valores estuvieron por debajo de los valores reportados y el Cd no fue registrado, en lo que respecta al Fe y Al no existen registros de estos metales en céstodos parásitos de organismos terrestres. Con los datos registrados en el presente trabajo …