Parasitology Commons^™

Laelapine Mite (Acari: Laelapidae) Morphometric Analysis Reflects Taxonomic And Geographic Clusters Of South American Oryzomyines (Rodentia: Sigmodontinae), Donald Gettinger, Robert D. Owen

MANTER: Journal of Parasite Biodiversity

Ongoing efforts to survey and inventory nonvolant small mammals across the Neotropics are beginning to reveal a highly structured and diverse fauna of rodents and marsupials. By increasing the foundation of specimens in museums around the world, it has become possible, for the first time, to evaluate and quantify the similarities and differences among small mammals collected from a broad range of geographic locali­ties, ecoregions, and habitats. Because ectoparasites were sampled in the process of collecting mammalian voucher specimens, we are able to study the laelapine mites (Acari: Laelapidae) associated with well-iden­tified and verifiable host specimens. Here, we evaluate morphometric …

Dna Barcoding And Taxonomy: Dark Taxa And Dark Texts, Roderic D. M. Page

Harold W. Manter Laboratory of Parasitology: Library Materials

Both classical taxonomy and DNA barcoding are engaged in the task of digitizing the living world.Much of the taxonomic literature remains undigitized. The rise of open access publishing this century and the freeing of older literature from the shackles of copyright have greatly increased the online availability of taxonomic descriptions, but much of the literature of the mid- to late- twentieth century remains offline (‘dark texts’). DNA barcoding is generating a wealth of computable data that in many ways are much easier to work with than classical taxonomic descriptions, but many of the sequences are not identified to species level. …

Unique Features Of A Global Human Ectoparasite Identified Through Sequencing Of The Bed Bug Genome, Joshua B. Benoit, Zach N. Adelman, Klaus Reinhardt, Amanda Dolan, Monica Poelchau, Emily C. Jennings, Elise M. Szuter, Richard W. Hagan, Hemant Gujar, Jayendra Nath Shukla, Fang Zhu, M. Mohan, David R. Nelson, Andrew J. Rosendale, Christian Derst, Valentina Resnik, Sebastian Wernig, Pamela Menegazzi, Christian Wegener, Nicolai Peschel, Jacob M. Hendershot, Wolfgang Blenau, Reinhard Predel, Paul R. Johnston, Panagiotis Ioannidis, Robert M. Waterhouse, Ralf Nauen, Corinna Schorn, Mark-Christoph Ott, Frank Maiwald, Subba R. Palli

Entomology Faculty Publications

The bed bug, Cimex lectularius, has re-established itself as a ubiquitous human ectoparasite throughout much of the world during the past two decades. This global resurgence is likely linked to increased international travel and commerce in addition to widespread insecticide resistance. Analyses of the C. lectularius sequenced genome (650 Mb) and 14,220 predicted protein-coding genes provide a comprehensive representation of genes that are linked to traumatic insemination, a reduced chemosensory repertoire of genes related to obligate hematophagy, host-symbiont interactions, and several mechanisms of insecticide resistance. In addition, we document the presence of multiple putative lateral gene transfer events. Genome …

Local Parasite Lineage Sharing In Temperate Grassland Birds Provides Clues About Potential Origins Of Galapagos Avian Plasmodium, Iris I. Levin, Rachel E. Colborn, Daniel Kim, Noah G. Perlut, Rosalind B. Renfrew, Patricia G. Parker

Environmental Studies Faculty Publications

Oceanic archipelagos are vulnerable to natural introduction of parasites via migratory birds. Our aim was to characterize the geographic origins of two Plasmodium parasite lineages detected in the Galapagos Islands and in North American breeding bobolinks (Dolichonyx oryzivorus) that regularly stop in Galapagos during migration to their South American overwintering sites. We used samples from a grassland breeding bird assemblage in Nebraska, United States, and parasite DNA sequences from the Galapagos Islands, Ecuador, to compare to global data in a DNA sequence registry. Homologous DNA sequences from parasites detected in bobolinks and more sedentary birds (e.g., brown-headed cowbirds …

Local Admixture Of Amplified And Diversified Secreted Pathogenesis Determinants Shapes Mosaic Toxoplasma Gondii Genomes, Hernan Lorenzi, Asis Khan, Michael S. Behnke, Sivaranjani Namasivayam, Lakshmipuram S. Swapna, Michalis Hadjithomas, Svetlana Karamycheva, Deborah Pinney, Brian P. Brunk, James W. Ajioka, Daniel Ajzenberg, John C. Boothroyd, Jon P. Boyle, Marie L. Dardé, Maria A. Diaz-Miranda, Jitender P. Dubey, Heather M. Fritz, Solange M. Gennari, Brian D. Gregory, Kami Kim, Jeroen P. J. Saeij, Chunlei Su, Michael W. White, Xing-Quan Zhu, Daniel K. Howe, Benjamin M. Rosenthal, Michael E. Grigg, John Parkinson, Liang Liu, Jessica C. Kissinger

Veterinary Science Faculty Publications

Toxoplasma gondii is among the most prevalent parasites worldwide, infecting many wild and domestic animals and causing zoonotic infections in humans. T. gondii differs substantially in its broad distribution from closely related parasites that typically have narrow, specialized host ranges. To elucidate the genetic basis for these differences, we compared the genomes of 62 globally distributed T. gondii isolates to several closely related coccidian parasites. Our findings reveal that tandem amplification and diversification of secretory pathogenesis determinants is the primary feature that distinguishes the closely related genomes of these biologically diverse parasites. We further show that the unusual population structure …

Rnai-Mediated Gene Knockdown By Microinjection In The Model Entomopathogenic Nematode Heterorhabditis Bacteriophora., Ramesh Ratnappan, Jonathan Vadnal, Melissa Keaney, Ioannis Eleftherianos, Damien O'Halloran, John M. Hawdon

Microbiology, Immunology, and Tropical Medicine Faculty Publications

BACKGROUND: Parasitic nematodes threaten the health of humans and livestock and cause a major financial and socioeconomic burden to modern society. Given the widespread distribution of diseases caused by parasitic nematodes there is an urgent need to develop tools that will elucidate the genetic complexity of host-parasite interactions. Heterorhabditis bacteriophora is a parasitic nematode that allows simultaneous monitoring of nematode infection processes and host immune function, and offers potential as a tractable model for parasitic nematode infections. However, molecular tools to investigate these processes are required prior to its widespread acceptance as a robust model organism. In this paper we …

Identification And Characterization Of Interactors Of Plasmodium Falciparum Pfpk6, An Atypical Protein Kinase, Andi J. Cummins

Honors Undergraduate Theses

Plasmodium falciparum, the organism that causes the most prevalent and most virulent cases of malaria in humans, poses a major health burden on the developing world, especially in the tropical regions of Sub-Saharan Africa, Southeast Asia, and Latin America. The burden of the disease is intensified by the fact that the parasite has developed widespread resistance to all current antimalarial therapies, such as chloroquine. This drug resistance underscores the need to develop novel therapeutics that target the parasite, but show low toxicity in the human host. Protein kinases, because of their integral roles in cell signaling networks, are considered to …

Identification Of Taenia Metacestodes From Mongolian Mammals Using Multivariate Morphometrics Of The Rostellar Hooks, Danielle M. Tufts, Nyamsuren Batsaikhan, Michael Pitner, Gábor R. Rácz, Altangerel Tsogtsaikhan Dursahinhan, Scott Lyell Gardner

Erforschung biologischer Ressourcen der Mongolei / Exploration into the Biological Resources of Mongolia, ISSN 0440-1298

Parasite diversity in and among various species of mammals within Mongolia is still poorly understood. The current paper focusses on a small part of the results of the Mongolian Vertebrate Parasite Project (MVPP), which entailed a broad-scale biodiversity survey of the vertebrates and their parasites of the Gobi and Altai regions of Mongolia. We report on the prevalence and morphological variation of larval cestodes of the family Taeniidae that occurred in small mammals that were collected from 2009-2012 from various locations in southern Mongolia. From these metacestodes, we studied both large and small rostellar hooks and analyzed both size and …

Der Mönchsgeier Aegypius Monachus (L., 1766) Und Seine Mallophagen (Insecta, Phthiraptera) In Der Mongolei = Cinereous Vulture Aegypius Monachus (L., 1766) And Its Chewing Lice (Insecta, Phthiraptera) In Mongolia, Eberhard Mey, Michael Stubbe, Davaa Lchagvasuren, Annegret Stubbe

Erforschung biologischer Ressourcen der Mongolei / Exploration into the Biological Resources of Mongolia, ISSN 0440-1298

During a series of studies on the population, ecology, and biology of the Cinereous Vulture in Mongolia, fully feathered nestlings of the species from 9 nests were examined, without the use of chemical methods, for Mallophaga infestation from 2010 to 2015. The collection resulted in several first records for Mongolia of three species: Laemobothrion vulturis (J. C. FABRICIUS, 1775) sensu lato (Amblycera, Laemobothriidae), Neocolpocephalum aegypii (TENDEIRO, 1989) (Amblycera, Menoponidae s. l.) sp. inq., and Falcolipeurus quadripustulatus (BURMEISTER, 1838) (Ischnocera, Philopteridae s. l.). Despite being expected, there has still been no record of Agypoecus brevicollis (BURMEISTER, 1838) in Mongolia.

Chewing louse …

Key To The Bat Fleas Of Mongolia, Ingo Scheffler

Erforschung biologischer Ressourcen der Mongolei / Exploration into the Biological Resources of Mongolia, ISSN 0440-1298

Fleas of the family Ischnopsyllidae belong to the common bat ectoparasites. The current taxonomic status of these insects in Mongolia includes seven species for which we provide a determination key.

Bat Ectoparasites Of Mongolia, Part 3, Ingo Scheffler, Ariunbold Jargalsaikhan, Idertsogt Bolorchimeg, Annegret Stubbe, Michael Stubbe, Andreas Abraham, K. Thiele

Erforschung biologischer Ressourcen der Mongolei / Exploration into the Biological Resources of Mongolia, ISSN 0440-1298

The fauna of Mongolian bats and their ectoparasites is not yet fully documented. This study analyzes ectoparasite samples of 15 bat species from diverse taiga, steppe, and desert locations. We recorded 27 parasite species in total and report their abundance, host preference, and occurrence herein. In describing a new bat fly species (Basilia dolchii n. spec.), reporting six parasite species never before recorded in Mongolia, and examining rare host species such as Myotis frater and Murina hilgendorfi, this work expands present knowledge in this research area. To assess respective individual and species-specific parasite loads comprised of different insects …

Modeling The Geographic Distribution Of Ixodes Scapularis And Ixodes Pacificus (Acari: Ixodidae) In The Contiguous United States, Micah B. Hahn, Catherine S. Jarnevich, Andrew J. Monaghan, Rebecca J. Eisen

Entomology Papers from Other Sources

In addition to serving as vectors of several other human pathogens, the black-legged tick, Ixodes scapularis Say, and western black-legged tick, Ixodes pacificus Cooley and Kohls, are the primary vectors of the spirochete (Borrelia burgdorferi) that causes Lyme disease, the most common vector-borne disease in the United States. Over the past two decades, the geographic range of I. pacificus has changed modestly while, in contrast, the I. scapularis range has expanded substantially, which likely contributes to the concurrent expansion in the distribution of human Lyme disease cases in the Northeastern, North-Central and Mid-Atlantic states. Identifying counties that contain …

Genomic Comparisons And Genome Architecture Of Divergent Trypanosoma Species, Katie Bradwell

Theses and Dissertations

Virulent Trypanosoma cruzi, and the non-pathogenic Trypanosoma conorhini and Trypanosoma rangeli are protozoan parasites with divergent lifestyles. T. cruzi and T. rangeli are endemic to Latin America, whereas T. conorhini is tropicopolitan. Reduviid bug vectors spread these parasites to mammalian hosts, within which T. rangeli and T. conorhini replicate extracellularly, while T. cruzi has intracellular stages. Firstly, this work compares the genomes of these parasites to understand their differing phenotypes. Secondly, genome architecture of T. cruzi is examined to address the effect of a complex hybridization history, polycistronic transcription, and genome plasticity on this organism, and study its highly …

Infection With The Sand Flea Tunga Penetrans (Tungiasis) In A Traveller Returning From Cameroon, Africa, D. J. Richardson, A. M. Mangili

Journal of the Arkansas Academy of Science

Tungiasis refers to human infection with adult fleas, Tunga penetrans. Although common throughout the tropics, tungiasis is rarely encountered by physicians in the United States such that it may be improperly diagnosed and inappropriately treated. We provide a case report of tungiasis in a traveler from Cameroon and a brief review of reported cases in the United States and Canada.

Cross-Sectional Analysis Of Salivary Cortisol And Cyathostome Infestation In Horses, Danielle L. Keating

Undergraduate Honors Thesis Projects

With an increase in anthelmintic resistance and decreased efficacy of many commercial dewormers, understanding factors that contribute to parasite infestations in horses is integral to their management. The goal of this study was to look at the potential relationship between parasites and stress response by evaluating salivary cortisol levels and cyathostome egg shedding levels. Using a sample size of n = 200 horses from the state of Ohio, fecal and saliva samples were collected from each horse. Fecal egg counts were performed for each horse with a modified Stoll method, and saliva samples were tested with an enzyme-linked immunosorbent assay …

The Utilization Of Morphological And Genetic Diagnostic Techniques For The Description Of Trematode Species Collected From Waterbirds From Lake Winnibigoshish, Minnesota, Usa, Tyler Joseph Achatz

All Graduate Theses, Dissertations, and Other Capstone Projects

Historically, morphological techniques for species identification were the leading diagnostic methodology, however, the increased usage of genetic techniques has led to a decrease in reports of morphometrics. The decrease in morphological reports increases the chance of missing diagnostic morphometrics. The three studies described herein used morphological and genetic diagnostic methods to identify trematodes from five families in order to improve genetic and morphological information for trematode species identification.

The first study identified ten species of trematodes from intestines of waterbirds previously collected from Lake Winnibigoshish, Minnesota. Nine of the species were sequenced for 28S ribosomal DNA (rDNA). Two species were …

Acanthocephala Of Arkansas, Including New Host And Geographic Distribution Records From Fishes, C. T. Mcallister, D. J. Richardson, M. A. Barger, T. J. Fayton, H. W. Robison

Journal of the Arkansas Academy of Science

Little is known about the spiny- or thorny-headed worms (Phylum Acanthocephala) of Arkansas and there are no summaries on the acanthocephalans of the state. Here, we provide a checklist of the 22 acanthocephalans that occur in Arkansas’ vertebrates based on previously published records and new data presented herein. In addition, we document a new state record as well as 13 new host records for some fish acanthocephalans.

The Ticks (Arachnida: Acari: Ixodida) Of Arkansas, C. T. Mcallister, L. A. Durden, H. W. Robison

Journal of the Arkansas Academy of Science

Although ticks are a nuisance to humans and other animals, they are an important part of the biota of North America. In addition, they are vectors of many tick-borne disease agents that can negatively affect higher vertebrates. In Arkansas, there have been no recent comprehensive summaries of the ticks (Acari: Ixodida) in the last 40+ yrs. Here, we provide a summary of the ticks of the state and note the disease agents they can transmit.

Endohelminth Parasites Of Some Midwater And Benthopelagic Stomiiform Fishes From The Northern Gulf Of Mexico, Michael J. Andres, Mark S. Peterson, Robin M. Overstreet

Gulf and Caribbean Research

: Mesopelagic fishes represent significant ecological links between mesozooplankton and the larger pelagic squids, fishes, and marine mammals. As such, these fishes also play a significant role as intermediate or paratenic hosts for parasites that require a crustacean intermediate host and mature in marine mammals or pelagic fishes. We examined a total of 208 individuals representing 5 species of Sternoptychidae and 88 individuals representing 2 species of Phosichthyidae from 20 locations in the northern Gulf of Mexico (nGOM). Six of the 7 species we examined are mesopelagic and one species was benthopelagic. We found the larval stages of Anisakis brevispiculata …

Genomic Insights Into The Ixodes Scapularis Tick Vector Of Lyme Disease, Monika Gulia-Nuss, Andrew B. Nuss, Jason M. Meyer, Daniel E. Sonenshine, R. Michael Roe, Robert M. Waterhouse, David B. Sattelle, Jose De La Fuente, Jose M. Ribeiro, Karine Megy

Biological Sciences Faculty Publications

Ticks transmit more pathogens to humans and animals than any other arthropod. We describe the 2.1 Gbp nuclear genome of the tick, Ixodes scapularis (Say), which vectors pathogens that cause Lyme disease, human granulocytic anaplasmosis, babesiosis and other diseases. The large genome reflects accumulation of repetitive DNA, new lineages of retro-transposons, and gene architecture patterns resembling ancient metazoans rather than pancrustaceans. Annotation of scaffolds representing ~57% of the genome, reveals 20,486 protein-coding genes and expansions of gene families associated with tick-host interactions. We report insights from genome analyses into parasitic processes unique to ticks, including host 'questing', prolonged feeding, cuticle …