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Expression Of Hcmv Ie1 In The U87mg Cell Line Augments Resistance To Temozolomide, Richard Rovin, Johnathan Lawrence, Justin Segula, Robert Winn 2009 Northern Michigan University

Expression Of Hcmv Ie1 In The U87mg Cell Line Augments Resistance To Temozolomide, Richard Rovin, Johnathan Lawrence, Justin Segula, Robert Winn

Johnathan Lawrence

INTRODUCTION: Human cytomegalovirus (HCMV) DNA and protein are found in gliomas but not in normal brain or other primary brain tumors. The role of HCMV infection in glioma biology is unclear. While it is unlikely that HCMV infection causes glioma, viral proteins might impart a proliferative and antiapoptotic phenotype that confers a survival advantage. Does this oncomodulation translate into a clinically relevant effect in glioma cells? To answer this question, we compared the response of the U87IE1 and U87MG malignant glioma cell lines to temozolomide. U87IE1 cells are U87MG cells that have been genetically engineered to produce HCMV IE1 protein. …


Regulatory Elements Of Xenopus Col2a1 Drive Cartilaginous Gene Expression In Transgenic Frogs, Ryan Kerney, Brian K. Hall, James Hanken 2009 Dalhousie University

Regulatory Elements Of Xenopus Col2a1 Drive Cartilaginous Gene Expression In Transgenic Frogs, Ryan Kerney, Brian K. Hall, James Hanken

Ryan Kerney

This study characterizes regulatory elements of collagen 2α1 (col2a1) in Xenopus that enable transgene expression in cartilage-forming chondrocytes. The reporters described in this study drive strong cartilage-specific gene expression, which will be a valuable tool for further investigations of Xenopus skeletal development. While endogenous col2a1 mRNA is expressed in many embryonic tissues, its expression becomes restricted to tadpole and adult chondrocytes. This chondrocyte-specific expression is recapitulated by col2a1 reporter constructs, which were tested through I-SceI meganuclease-mediated transgenesis. These constructs contain a portion of the Xenopus tropicalis col2a1 intron, which aligns to a cartilage-specific intronic enhancer that has been well characterized …


The Apoptotic Effect Of 1 ' S-1 '-Acetoxychavicol Acetate From Alpinia Conchigera On Human Cancer Cells, Noor Hasima Nagoor 2009 University of Malaya

The Apoptotic Effect Of 1 ' S-1 '-Acetoxychavicol Acetate From Alpinia Conchigera On Human Cancer Cells, Noor Hasima Nagoor

Noor Hasima Nagoor

1'-(S)-1'-Acetoxychavicol acetate (ACA) isolated from the Malaysian ethnomedicinal plant Alpinia conchigera Griff. was investigated for its potential as an anticancer drug. In this communication, we describe the cytotoxic and apoptotic properties of ACA on five human tumour cell lines. Data from MTT cell viability assays indicated that ACA induced both time-and dose-dependent cytotoxicity on all tumour cell lines tested and had no adverse cytotoxic effects on normal cells. Total mortality of the entire tumour cell population was achieved within 30 hrs when treated with ACA at 40.0 mu M concentration. Flow cytometric analysis for annexin-V and PI dual staining demonstrated …


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