Poultry or Avian Science Commons^™

Effects Of Intradermal Injections Of Lipopolysaccharide On The Local And Systemic Acute Inflammatory Responses In Turkeys, Delaney F. Roberts

Poultry Science Undergraduate Honors Theses

In chickens, the growing feather (GF) pulp has been established as a cutaneous bioassay to monitor and assess tissue/cellular immune activities in response to intradermal (i.d.) injections of test materials. The objective of this pilot study was to determine the suitability of the GF bioassay in turkeys by examining the acute inflammatory responses initiated by i.d. GF-pulp injections of lipopolysaccharide (LPS). For this, three, 5-wk-old turkey poults were used. Two poults had 8 GFs injected (10 μL/GF) with low (L)-LPS (0.1 μg/GF) and 8 GFs with high (H)-LPS (1 μg/GF), while a third poult had 8 GFs injected with endotoxin-free …

Inflammatory Response In Ucd200/206 Scleroderma-Prone Chickens To Intradermal Injection Of Silikon 1000, Katee L. Johnson

Animal Science Undergraduate Honors Theses

Intradermal injections of silicone materials have resulted in chronic inflammation and fibrotic reactions in some individuals. The UCD200/206 line of chickens is characterized by spontaneous development of scleroderma/systemic sclerosis (SSc), a fibrotic autoimmune disease. This longitudinal study examined the cellular inflammatory activity initiated by SILIKON 1000 (a silicone product used as a filler in human skin) at the site of intradermal (i.d.) injection and in the peripheral blood in the SSc-prone UCD 200/206 (UCD) chickens and healthy White Leghorn (WL) controls. Specifically, the pulps of growing feathers (GFs) were i.d. injected with SILIKON or endotoxin-free, sterile PBS (8 and 3 …

The Effect Of 1,25-Dihydroxyvitamin D3 Supplementation On Humoral Immunity Against Bacterial Chondronecrosis With Osteomyelitis Lameness In Broiler Chickens, Bailey Herberger

Animal Science Undergraduate Honors Theses

Bacterial chondronecrosis with osteomyelitis (BCO) lameness, the most predominant locomotive illness in fast-growing broilers, causes substantial animal well-being issues and affects broiler production, leading to huge financial losses. Several strategies, including feed supplementations using organic trace minerals, vitamin D, probiotics, and prebiotics, have been utilized to alleviate BCO lameness incidences in broiler chickens. Previous studies have indicated that feeding an additive of Panbonis^®-G-1,25(OH)₂D₃ from Solanum glaucophyllum containing 1 µg/kg G-1,25(OH)₂D₃ to broilers for the first 28 days of their production cycle reduced BCO lameness by more than 50%. In this study, we …

Evaluation Of Fecal Egg Counts (Fec) As A Viable Means To Estimate Ascaridia Galli, Heterakis Gallinarum And Capillaria Obsignata Burdens In Laying Hens, Alexander H. Moss

Animal Science Undergraduate Honors Theses

The control of gastrointestinal helminths, specifically Ascaridia galli, Heterakis gallinarum, and Capillaria obsignata, is a critical component of poultry health management, particularly in free-range and organic farming systems. Methods of estimating parasite burdens, such as post-mortem examinations, are not feasible for large-scale commercial operations due to their invasiveness and time requirements. This study aimed to evaluate the use of fecal egg counts (FEC) as a non-invasive and efficient method for estimating the worm burdens of these parasites in laying hens. A flock of 12 brown egg laying, spent, hens were individually penned on concrete. Fecal samples (both cecal and intestinal) …

The Expression Of Intestinal Tight Junction Proteins Induced By 1,25-Dihydroxyvitamin D3-Glycosides Supplementation To Reduce Bacterial Chondronecrosis With Osteomyelitis Lameness In Broilers, Kennedy M. Hicks

Biological Sciences Undergraduate Honors Theses

Bacterial Chondronecrosis with Osteomyelitis (BCO) is a leading cause of lameness in broiler chickens, impacting animal welfare and commercial poultry production. Supplementation using organic trace minerals, vitamin D, probiotics, and prebiotics has been studied to mitigate BCO lameness incidences in broiler chickens. Our previous study demonstrated that feeding Panbonis®-G-1,25(OH)₂D₃ from Solanum glaucophyllum, containing 1 µg/kg G-1,25(OH)₂D₃, to broilers for the first 28 days of their production cycle reduced BCO lameness by approximately 50%. In this study, we investigated the impact of 1,25-dihydroxy vitamin D₃ supplementation on the expression of tight junction …

A Comparative Study Of Urban And Natural Nesting Gray Hawks In The Lower Rio Grande Valley Of South Texas, Evan Farese

Theses and Dissertations

Urbanization can negatively impact birds of prey, which occupy higher trophic levels. The Gray Hawk (Buteo plagiatus) is a neotropical raptor inhabiting the Lower Rio Grande Valley (LRGV) of Texas, where it is threatened. The species’ ecology has been little studied and it is unclear how urbanization may affect population viability. I studied Gray Hawk reproductive success, diet, and thermal ecology at nests in urban and natural habitats in the LRGV. Camera trap data showed adults at urban nests delivered mostly avian prey, while mammalian prey was most frequently brought to natural nests. Data from temperature loggers showed …

Sustainable Poultry Farming: A Concept Of Iot-Based Poultry Management System For Small-Scale Farmers, Ayodeji Akinsoji Okubanjo, Ignatius Kema Okakwu, Olufemi Peter Alao, Nurudeen Samuel. Lawal, Ayoola Abiola Babalola, Abisola Olayiwola

Al-Bahir

Conventional poultry management techniques are failing to meet increased demand for poultry products as the population continues to grow. As a result, this issue has become a major concern for small-scale farmers, particularly those in low-income areas, in terms of food security. One of the main reasons for this is that the farmers rely on intensive farming methods which are inefficient for automating daily poultry operations. However, intensive farming methods pose major environmental concerns to ecosystems and poultry health. Also, the environmental conditions, welfare, and productivity of poultry operations may be harmed by the global climate crisis and poultry waste …

Mdpk Cell Lines Can Be Used For In Vitro Antiviral Testing With Highly Pathogenic Avian Influenza (H5n1), Jada L. Westover

Student Research Symposium

• Outbreak in agricultural poultry starting in 2022
• In March 2024 first confirmed case in Dairy Cattle
• Tested drugs T705, Oseltamivir, Baloxivir, Ribavirin, EIDD-2947, and EIDD-2749 on three different cell lines
• Goal: See if MDBK cell lines could be used for in vitro studies

Effect Of Heat Stress On Ileal Epithelial Barrier Integrity In Broilers Divergently Selected For High- And Low-Water Efficiency, Elizabeth S. Greene, Brooklee Roach, Maria Fernandez Cuadrado, Sara Orlowski, Sami Dridi

Poultry Science Faculty Publications and Presentations

Water scarcity and rising global temperatures are two of the greatest current and future threats to poultry sustainability. Therefore, selection for water efficiency (WE) and heat resilience are of vital importance. Additionally, intestinal integrity is of critical importance under challenging conditions to maintain nutrient absorption and therefore, growth and performance of broilers. Here, we examined the effect of chronic cyclic heat stress (HS) on the ileal expression profile of tight-junction, gap-junction, adherens, and desmosome genes in the fourth generation of divergently selected low (LWE)- and high water efficient (HWE)-chicken lines. LWE birds exhibited higher levels of gut permeability, regardless of …

Evaluation Of The Effect Of In Ovo Applied Bifidobacteria And Lactic Acid Bacteria On Enteric Colonization By Hatchery-Associated Opportunistic Pathogens And Early Performance In Broiler Chickens, Mitchell C. Rowland, Aaron J. Forga, James Higuita, Makenly E. Coles, Billy M. Hargis, Christine N. Vuong, Danielle Graham

Poultry Science Faculty Publications and Presentations

Probiotics have been used to promote pioneer colonization by beneficial bacteria in poultry. The purpose of the present study (four trials) was to determine if an in ovo injection with Bifidobacterium saeculare (B2-2, B3-4) and/or lactic acid bacteria (LAB18, LAB46) at d18 of embryogenesis reduced Enterococcus and Gram-negative bacterial colonization in the gut at hatch. An environmental challenge model was used to simulate microbial contamination in large-scale hatch cabinets in a laboratory setting. In all trials, gut homogenates obtained from chicks at hatch were plated to enumerate relevant bacterial populations. Performance was evaluated in trials two and four. The top …

The Mechanistic Target Of Rapamycin Mediates Clostridium Perfringens-Induced Chicken Necrotic Enteritis Attenuated By Secondary Bile Acid Deoxycholic Acid, Mohit Bansal, Tahrir Alenezi, Ying Fu, Janashrit Shrestha, Ayidh Almansour, Hong Wang, Anamika Gupta, Rohana Liyanage, Xiaolun Sun

Poultry Science Faculty Publications and Presentations

Clostridium perfringens is a prevalent gut bacterial pathogen in humans and animals. This study investigated the role of the mechanistic targets of rapamycin (mTOR) and deoxycholic acid (DCA) on C. perfringens intestinal infection. Chickens were sequentially infected with Eimeria maxima and received the mTOR inhibitor rapamycin and DCA. C. perfringens-induced necrotic enteritis (NE) was evaluated using body weight gain (BWG), histopathology, bile acids, pathogen colonization, cell infiltration and death, and gene expression. The significant difference of p < 0.05 was analyzed by one-way ANOVA and multiple comparisons. Notably, rapamycin strongly reduced the subclinical and clinical NE histopathologies. DCA and DCA combined with rapamycin alleviated clinical NE and BWG loss. Rapamycin, DCA, and DCA + rapamycin attenuated bile acid reduction in NE birds, and they also reduced immune cell infiltration into the intestinal lamina propria as well as immune cell migration in vitro. At molecular levels, DCA and DCA + rapamycin reduced proinflammatory IFN gamma, MMP9, IL23, and IL17 gene expression. Rapamycin, DCA, and DCA + rapamycin reduced NE-induced intestinal cell apoptosis. Together, these results suggest that mTOR signaling mediates C. perfringens-induced ileitis, and combining mTOR inhibition and DCA improves the intervention efficacy against NE ileitis and BWG loss.

Hatching And Caring For Chicks, Cory Farnsworth, Jacob Hadfield, Ashley Longmore

All Current Publications

There are many reasons people are interested in raising birds. Most people who desire to start are interested in saving money and becoming more self-sufficient. Many people find excitement in hatching eggs and raising chicks. This fact sheet will help you get started with hatching eggs and raising chicks.

Empowering Young Innovators Through School-Based Agricultural Education: Insights From A Nigerian Secondary School Teacher, Mary O. Olumide-Oyaniyi

National Collaborative for Research on Food, Energy, and Water Education (NC-FEW)

Nigeria faces challenges like food insecurity and economic instability, worsened by a growing youth population. School-Based Agricultural Education (SBAE) engages youth in agricultural innovations. This study implemented SBAE principles in a Nigerian school, combining poultry farming and aquaculture. Students managed a poultry farm, using chicken droppings to breed maggots as feed for catfish in rainwater-filled tanks. The project enhanced students' skills, improved local food production, and engaged the community. Despite funding and management challenges, the initiative demonstrated SBAE's potential to empower youth and advance agricultural practices, offering a model for similar projects in other regions.

The Nebraska Bird Review (March 2025) 93(1), Whole Issue

Nebraska Bird Review

Winter Field Report, December 2024–February 2025, by W. Ross Silcock
2024–2025 Christmas Bird Counts, by Don Paseka
Annual Color Photo Section
Annual Treasurer’s Report for 2024, by Jan Johnson
Subscription and Organization Information

Annual Color Photo Section

Nebraska Bird Review

The following seven pages show examples of this year’s theme: birds feeding or carrying food.

Osprey with striped bass
Spotted Sandpiper with beetle
Scissor-tailed Flycatcher with insect
Male Pine Grosbeak with green ash seeds
Merlin eating sparrow
Whooping Crane carrying ear of corn
Yellow-billed Cuckoo with harvestman spider
Black-billed Magpie eating grain
Red-tailed Hawk with plains garter snake
Summer Tanager with hackberry emperor butterfly
Cattle Egret with bullfrog
Juvenile Red-shouldered Hawk eating garter snake
Red-breasted Merganser eating fish
Juvenile White Ibis eating unidentified item
Bohemian Waxwing eating crab apples
Rose-breasted Grosbeak eating sunflower seeds
Loggerhead Shrike eating dragonfly
Yellow-crowned Night …

2024–2025 Christmas Bird Counts, Don Paseka

Nebraska Bird Review

For the second year in a row, all twenty CBCs [Christmas Bird Counts] were run during the 2024–2025 season. The weather was generally cooperative, although five circles reported fog and two others had rain. Branched Oak-Seward was postponed a week due to icy conditions on the originally scheduled day. The average high temperature for all circles on count day was 41 and the average low temperature was 22. Because December had remained relatively mild, there was a good deal of open water, which encouraged many species of water birds to remain. Consequently, most of the circles reported species totals higher …

Nebraska Ornithologists’ Union Treasurer’S Report, December 31, 2024, Jan Johnson

Nebraska Bird Review

No abstract provided.

Winter Field Report, December 2024–February 2025, W. Ross Silcock

Nebraska Bird Review

Since I started a few years ago to compare each season’s reports with the status of each species as presented in BONO (Species Accounts – Birds of Nebraska – Online), I had expected that “Routine reports” would apply to an increasing number of species as the BONO database firmed up, but this hasn’t happened. My comments from last winter continue to be valid this winter, now for the third winter in a row: As was the case for winter 2022–2023, the big general story was the large number of reports of tardy fall migrants and unexpectedly northerly occurrences in mid-winter… …

Influence Of High Eimeria Tenella Immunization Dosages On Total Oocyst Output And Specific Antibodies Recognition Response In Hybrid Pullets (Gallus Gallus)-A Pilot Study, Marco A. Juarez-Estrada, Guillermo Tellez-Isaias, Victor M. Petrone-Garcia, Amanda Gayosso-Vazquez, Xochitl Hernandez-Velasco, Rogelio A. Alonso-Morales

Political Science Faculty Publications and Presentations

Background: Two high primary-immunization doses of a wild-type E. tenella strain were assessed in healthy pullets (5K versus 10K sporulated oocysts/bird) to understand the effects of coccidia infection. Methods: Acquired immunity was evaluated following primary immunization and two booster doses with the homologous strain. Total oocyst shedding, clinical signs, and viability of every bird/group after each immunization/booster were recorded. Indirect ELISA measured the time course of humoral responses from each immunization group against sporozoite and second-generation merozoite of E. tenella. Antigen pattern recognition on these two asexual zoite stages of E. tenella was analyzed using Western blotting with antibodies from …

Kinetics Of Protein And Amino Acids, S. Y. Liu, M. Toghyani, S. P. Macelline, P. H. Selle

Proceedings of the Arkansas Nutrition Conference

Protein and amino acid nutrition is key to poultry growth and sustainability, with synchrony between glucose and amino acid availability being critical for efficient feed conversion. While non-bound amino acids (NBAA) are rapidly absorbed and increasingly used to reduce crude protein levels, their fast uptake may disrupt amino acid balance and reduce nutrient utilization compared to protein-bound amino acids. Future research should redefine amino acid requirements in low-CP diets, explore the metabolic fate of NBAA, and investigate how protein sources affect gut microbiota and host metabolism.