Animal Sciences Commons^™

Isolation And Characterization Of Microsatellite Loci From Wild Striped Snakehead (Channa Striata)., Yasmeen Sameer Mahmoud Salah

Student Works (2010-2019)

Channa striata (better known as Haruan in Malaysia), is a freshwater species that is well known as a protein source and also for its pharmaceutical value among wounded patients. Unfortunately stocks have declined over the past few years, thus an in-depth study is needed to rule out genetic irregularities that might be causing the slow extinction of the species. This study was conducted for the purpose of studying the population diversity and genetic structure of Channa striata (striped snakehead) in seven different locations around the peninsular of Malaysia. Microsatellite markers were isolated and identified using Random Amplified Microsatellite (RAM) primers. …

Isolation And Development Of Microsatellite Markers Of Channa Striata., Faiza Omar

Student Works (2010-2019)

Channa striata, locally known as haruan has long been regarded as a valuable edible fish and it is also consumed for its putative effects on wound healing. The main objective of the project was to develop microsatellite markers for use in the study of genetic diversity of the wild Channa striata populations in Malaysia. In this study, seven new microsatellite loci were isolated from Channa striata using a Random Amplified Microsatellite (RAMs) based technique which was an efficient and reliable method. Ten primers were used in population genetic studies of Channa striata.Sixty sets of microsatellite primer pairs were used in …

Biofouling And Short-Term Dynamics On Fish Cage Netting In Relation To Fish Rearing And Environmental Factors., John Madin

Student Works (2010-2019)

Four studies were conducted in a floating net-cages farm at Matang Mangrove Forest Reserve (MMFR), Perak, Malaysia, to elucidate the biofouling assemblages on cage nettings and factors that influence their development. In the first study (Chapter 3),biofouling on 1.6 cm mesh net panels (size 0.2 m x 2 m) suspended inside (homemade pellet ‘P’, trash fish ‘T’) and outside (O) experimental net-cages in a fish farm were monitored every week until net openings were completely occluded by macrofouling organisms. Eight species (7 phyla) of sessile organisms and 27 species (3 phyla) of nonsessile associates were recorded.Colonization by macrofouling organisms usually …

Effects Of Different Extenders, Cryoprotectants, Equilibration And Vapour Exposure On Freezability Of African Catfish(Clarias Gariepinus) Sperm., Noor Azlina Kamaruding

Student Works (2010-2019)

The aim of this study was to develop an optimal freezing protocol for African catfish(Clarias gariepinus) sperm with special reference to type of extender and cryoprotectant, molarity, equilibration duration, vapour temperature and vapour exposure duration. Using Tris-Citric Acid Yolk Extender (TCAYE), a 3x3x3x3 factorial experiment was carried out consisting of 3 molarities of glycerol (0.5, 1.0 and 2.0 M), 3 equilibration durations (120,140 and 160 minutes), 3 vapour temperatures (-80, -90 and -100oC) and 3 vapour exposure durations (5, 10 and 15 minutes). In addition, using Fish-Ringer Extender (FRE), a 3x3x3 factorial experiment was also conducted involving 3 equilibration durations …

Robert Edward Tucker, Sr. Papers, Zach S. Henderson Library Special Collections

Finding Aids

This collection consists of Robert Edward Tucker, Sr.’s ornithology field notes and journals concerning observations in the Chatham County area & the Georgia coastal islands. Most materials are handwritten and include daily observations from 1984-1997.

Find this collection in the University Libraries' catalog.

Lobster Attack Induces Sensitization In The Sea Hare, Aplysia Californica, Amanda J. Watkins, Daniel A. Goldstein, Lucy C. Lee, Christina J. Pepino, Scott L. Tillett, Francis E. Ross, Elizabeth M. Wilder, Virginia A. Zachary, William G. Wright

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Studies of the neural mechanisms of learning, especially of sensitization, have benefitted from extensive research on the model species, Aplysia californica (hereafter Aplysia). Considering this volume of literature on mechanisms, it is surprising that our understanding of the ecological context of sensitization in Aplysia is completely lacking. Indeed, the widespread use of strong electric shock to induce sensitization (an enhancement of withdrawal reflexes following noxious stimulation) is completely unnatural and leaves unanswered the question of whether this simple form of learning has any ecological relevance. We hypothesized that sublethal attack by a co-occurring predator, the spiny lobster, Panulirus interruptus, might …

Pathological And Clinical Findings And Tissue Distribution Of Salmonella Gallinarum Infection In Turkey Poults, Lati̇fe Beyaz, Ayhan Atasever, Fuat Aydin, K. Semi̇h Gümüşsoy, Seçi̇l Abay

Turkish Journal of Veterinary & Animal Sciences

This study aimed to experimentally induce Salmonella enterica subsp. enterica serovar Enteritidis (Salmonella Gallinarum) infection in turkey poults in order to detect S. Gallinarum using immunohistochemical, bacteriological, and histological procedures. The study included 90 white turkey poults that were divided into 3 groups (1-day-old, 3-week-old, and 2-month-old), each of which was orally inoculated with an inoculum of 0.3 mL of S. Gallinarum strain 9 broth culture that contained approximately 2 × 10^4, 1 × 10^6, and 1 × 10^9 cfu/mL, respectively. The duration of the experiment was 18 days. Clinical, pathological, and bacteriological findings were evaluated daily until 18 days …

Contemporary Grouping In Mixed-Size Dairy Herds Experiencing Four Seasons, Mohammad Ali̇ Nilforooshan

Turkish Journal of Veterinary & Animal Sciences

Countries with different herd structures and climates use different combinations of herd, year, and season to define the effect of their climatic conditions and management. Estimates of variance components and Akaike information criterion values resulting from 8 analytical models differentiated by different combinations of herd, year and season effects were compared for milk and fat yields. Data consisted of pedigree and records of 11,850 Holsteins freshening in 38 dairy herds of Isfahan, Iran, between January 1994 and October 2001. Heritability estimates were lowest for the models that used herd-year combination as a random effect. Moreover, the use of these models …

Immune Response To Type-2 Porcine Circovirus In Weaned Pigs, Hong Chen, Ke-Ying Zhang, Xue-Mei Ding, Zhi-Wen Xu, Dai-Wen Chen, Gang Tian

Turkish Journal of Veterinary & Animal Sciences

Sixteen crossbred barrows (Duroc × Landrace × Yorkshire) weaned at 28 days with an initial body weight of 5.12-8.64 kg and no antibodies to PCV2 were used in a 5-week trial to evaluate the immune response after intranasal inoculation with type-2 porcine circovirus. The percentage of peripheral blood ANAE+ T cells, PCV2 antibody titer, immunoglobulins, and serum IFN-\gamma, IL-2, and sIL-2R levels were determined. The results show that the percentage of peripheral blood ANAE+ T cells was notably higher in inoculated pigs 5 days post-inoculation (dpi) and significantly lower 21 dpi than in the control group. The anti-PCV2 titer in …

The Performance Of A Small-Scale, High-Density, Continuous System For Culturing The Rotifer Brachionus Plicatilis, Umur Önal, İhsan Çeli̇k, Sebahatti̇n Ergün

Turkish Journal of Veterinary & Animal Sciences

The performance of a high-density continuous system was evaluated as an alternative to conventional low-density batch-culture systems for culturing the rotifer Brachionus plicatilis. Rotifer densities ranging from 1700 to 3760 individuals/mL were achieved in 4 different trials that lasted 10-18 days. Problems associated with NH_3-N and pH were controlled by increasing the water exchange rate and periodic addition of ammonia remover. While NH_3-N and pH values were 0-8.8 mg/L and 6.9-9.3, respectively, the most important problem was the accumulation of TSS at concentrations of up to 85 mL/L within a very short time after start-up. Further studies are required to …

Reference Values For Some Haematological, Haematochemical, And Electrophoretic Parameters In The Girgentana Goat, Giuseppe Piccione, Stefania Casella, Lucio Lutri, Irene Vazzana, Vincenzo Ferrantelli, Giovanni Caola

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to determine haematological, haematochemical, and electrophoretic reference values for the Girgentana goat in order to form a basis for clinical interpretation. The study included 348 female Girgentana goats aged from 1 to 6 years. The animals were divided into 3 groups: goats aged 1-2 years (group A), goats aged 3-4 years (group B), and goats aged 5-6 years (group C). Blood samples were collected from each animal every morning at the same time (0800) by means of jugular venipuncture, and red blood cell (RBC), white blood cell (WBC), neutrophil (NEU), lymphocyte (LYM), monocyte (MONO), …

A Common Tortoise Tick, Hyalomma Aegyptium Linne 1758 (Acari: Ixodidae), Identified On Eastern Hedgehog (Erinaceus Concolor Martin 1838) In Central Anatolia, Serkal Gazyağci, Nursel Aşan, Yasi̇n Demi̇rbaş

Turkish Journal of Veterinary & Animal Sciences

Ticks are obligate blood sucker arthropods that infect animals and humans. A common tortoise tick, Hyalomma aegyptium, was collected from a young and an adult male hedgehog, Erinaceus concolor, from Central Anatolia in July 2008. More ticks were determined on the young one. This is the second record of tortoise tick that parasitizes a hedgehog.

Conception Rates For Standing Estrus And Fixed-Time Insemination In Dairy Heifers Synchronized With Gnrh And Pgf_{2\Alpha}, Samir Z. El-Zarkouny

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to evaluate the effectiveness of GnRH PGF-based protocols on reproductive outcome in Holstein heifers. Heifers were randomly assigned to the control group (n = 14), or treatment groups (n = 50) treated with GnRH (day -7) and PGF_{2\alpha} (day 0). Heifers that exhibited estrus were inseminated (Select Synch, n = 34) and heifers that did not exhibit estrus up to 48 h after PGF_{2\alpha} injection received a second GnRH injection and were fixed-time inseminated (Ovsynch, n = 16). Conception rates were 40%, 55.9%, and 31.3%, and overall pregnancy rates (P < 0.05) were 64.3%, 97%, and 87.5% in the control, Select Synch, and Ovsynch groups, respectively. Ovsynch heifers had 1.8 ± 0.5 and 3.2 ± 0.6 ng mL^{–1} of serum P4, versus 3.3 ± 0.5 and 3.8 ± 0.6 ng mL–1 in the Select Synch heifers at the time of the first GnRH and PGF_{2\alpha} injection, respectively. Conception rates were 55.6% and 58.3% when P4 concentrations were high, and were 50% and 25% when P4 concentrations were low at the time of the first GnRH and PGF_{2\alpha} injection, respectively. Dairy heifers synchronized with GnRH and PGF_{2\alpha} should be inseminated when estrus is observed, and heifers that do not exhibit estrus up to 48 h after PGF_{2\alpha} injection should be given a second GnRH injection and fixed-time inseminated 16-20 h later.

Effect Of Pre-Evisceration, Skin-On Carcass Decontamination Sanitation Strategies For Reducing Bacterial Contamination Of Cattle During Skinning, Mehmet Çalicioğlu, Dennis R. Buege, John B. Luchansky

Turkish Journal of Veterinary & Animal Sciences

The effectiveness of pre-evisceration, skin-on carcass sanitation on reducing bacterial contamination of beef carcasses was tested using 3 cattle per treatment and 3 cattle as controls at each of 3 abattoirs in southern Wisconsin. The sanitation procedure included stunning, bleeding, tying off the esophagus, sealing the anus, and then sanitizing the hide with: i) 20% trisodium phosphate, ii) 200 ppm iodophor, iii) 75% ethanol, or iv) hot water (ca. 80 °C). Two sets of combined sponge samples (3 × 100 cm^2) were taken from the hide before and after the sanitation step, as well as from the carcass after the …

All-Trans Retinoic Acid Regulates Intracellular Redox Status In Marc-145 Cells Infected With Porcine Reproductive And Respiratory Syndrome Virus, Yan Lin, De Wu

Turkish Journal of Veterinary & Animal Sciences

A relationship between oxidative stress, and apoptosis and/or death has been suggested for many years. The objective of this study was to determine the influence of all-trans retinoic acid (ATRA) on the cell survival rate and the antioxidant defense system. MARC-145 cells were treated with ATRA after infection with porcine reproductive and respiratory syndrome virus (PRRSV). The cell survival rate, total antioxidant capability (T-AOC), glutathione peroxidase (GSH-P_X) activity, superoxide dismutase (SOD) activity, lactate dehydrogenase (LDH) activity, and glutathione (GSH) content were determined in samples collected from cells incubated with ATRA for 24 or 48 h. The results show that PRRSV …

Maedi-Visna Virus In Turkish Sheep: A Preliminary Serological Survey Using Elisa Tests, Silvia Preziuso, Mehmet Erman Or, Monica Giammarioli, Abdullah Kayar, Francesco Feliziani, Remzi̇ Gönül, Silvana Farneti, Cağla Parkan Yaramiş, Carlo Valente, Vincenzo Cuteri

Turkish Journal of Veterinary & Animal Sciences

The maedi-visna virus (MVV) is distributed worldwide and, to date, no epidemiological surveys have been carried out using ELISA testing in Turkey. The aims of this study were as follows: i) to verify the diagnostic performance of a home-made ELISA test (HM-ELISA) using Turkish ovine sera in comparison with other commercially available ELISA tests, and ii) to perform a preliminary epidemiological survey in İstanbul province. The MVV seroprevalence in İstanbul province was 15.3%. Older sheep were 5 times more likely to be positive than younger animals, ewes were 3 times more likely to be positive than rams, and all the …

Nasal Squamous Cell Carcinoma In A Cow, Ali Baniadam, Navid Moezzi, Babak Mohammadian

Turkish Journal of Veterinary & Animal Sciences

A patient with a turgid, hemorrhagic mass infected by myiasis in the left nostril and diagnosed as squamous cell carcinoma (SCC) is presented. Complete blood count (CBC) showed moderate neutrophilia and eosinophilia and vital signs were in normal range. After aseptic surgical preparations and local anesthesia, the mass removed en bloc and submitted to the Department of Veterinary Pathology for histopathological evaluation. SCC was recognized microscopically by identifying malignant epithelial cells demonstrating various degrees of differentiation towards keratinocytes.

Impairments In Haematological Parameters Of Saprolegnia Spp. Infected Tench,Tinca Tinca, Syed Lal Shah

Turkish Journal of Veterinary & Animal Sciences

Haematological parameters of tench (Tinca tinca) infected by Saprolegnia spp. were studied. All parameters, except mean corpuscular volume (MCV), decreased in response to infection. A significant (P < 0.05) decrease was observed in haemoglobin (Hb) (-26.85%), red blood cell count (RBCc) (-20.94%), leucocrit (Lct) (-24.14%), and mean corpuscular haemoglobin concentration (MCHC) (-9.06%). Haematocrit (Hct), white blood cell count (WBCc), and mean corpuscular haemoglobin (MCH) also decreased (-7.66%, -12.44%, and -6.19%, respectively); however, differences between the infected and control groups were not significant. Among the parameters, only MCV had a non-significant increase (+7.84%) in the infected fish. The study results show that saprolegniasis caused anaemia and immunosuppression, followed by mortality in tench. Mortality was attributed to haemodilution caused by haemorrhaging and the breakdown of osmotic balance due to tissue (skin and muscular layer) destruction via the penetration of the hyphae; however, lethargy was another mortality promoting factor. Mortality among the Saprolegnia-infected fish also depended upon the initial site of infection, type of tissue destroyed, growth rate of the fungus, and ability of individual fish to withstand the stress. The present study was conducted to investigate physiological impairment in Saprolegnia-infected Tinca tinca under laboratory conditions; however, further studies at the population level in natural habitats and under similar stress conditions are needed.

Effects Of Feed Refreshing Frequency On Behavioural Responses Of Awassi Lambs, Mahmut Keski̇n, Ahmet Şahi̇n, Sabri̇ Gül, Osman Bi̇çer

Turkish Journal of Veterinary & Animal Sciences

Sixty 2-month-old, male Awassi lambs were used in this study to investigate the effects of feed refreshing frequencies on behavioural responses of lambs. Treatments consisted of feed refreshing at intervals of 2 h (FR2), 4 h (FR4), 8 h (FR8), 12 h (FR12), and 24 h (FR24). Each lamb within each group kept individually was considered a replicate. All lambs were offered fresh feed ad libitum in each refreshing period for 56 days. Behavioural responses of the lambs were determined individually by monitoring the animals 2 days a week for 30 min at 10:00, 12:00, 14:00, 16:00, 18:00, 20:00, 22:00, …

Certain Meat Quality Characteristics Of Awassi And Turkish Merino × Awassi (F_1) Lambs, Romedi̇ Çeli̇k, Alper Yilmaz

Turkish Journal of Veterinary & Animal Sciences

This study aimed to determine the meat quality characteristics of 2-way crossbred lambs that were obtained by mating Turkish Merino (Karacabey Merino) as a sire line with Awassi ewes, as compared to those of purebred Awassi lambs. In all, 8 male and 8 female single born lambs from each genotype (forming 4 groups and 32 lambs) were investigated. In Awassi purebred and Turkish Merino × Awassi (F_1) crossbred lambs mean pH values at 24 h were 5.84 and 5.80, drip losses were 2.35% and 2.69%, cooking losses were 31.41% and 30.94%, Warner-Bratzler shear force values were 3.42 kg and 2.63 …