Animal Sciences Commons^™

Ecology And Relationships Of Rhabdias Spp. (Nematoda: Rhabdiasidae) From North American Amphibians And Reptiles, Gabriel J. Langford

School of Biological Sciences: Dissertations, Theses, and Student Research

Lungworms of the cosmopolitan genus Rhabdias (Nematoda: Rhabdiasidae) are among the most common parasites of amphibians and squamate reptiles. The life cycles, life histories, host specificities, and evolutionary relationships of Rhabdias spp. were studied through examination of their free-living and parasitic stages in amphibians and reptiles. This study found free-living development of anuran lungworms was primarily limited to heterogonic reproduction, whereas snake lungworms primarily reproduced homogonically. Infective anuran lungworms penetrated the skin of frogs and toads; in contrast, snake lungworms penetrated snake esophageal tissue during per os infections. Our molecular phylogeny strongly supported separate clades for anuran and snake lungworms, …

Effects Of Variable Oceanographic Conditions On Forage Fish Lipid Content And Fatty Acid Composition In The Northern California Current, Marisa N. C. Litz, Richard D. Brodeur, Robert L. Emmett, Selina S. Heppell, Rosalee S. Hellberg, Linda O'Higgins, Matthew S. Morris

Food Science Faculty Articles and Research

Lipids and fatty acids (FA) were investigated in 4 species of forage fish: northern anchovy Engraulis mordax, Pacific sardine Sardinops sagax, Pacific herring Clupea pallasi, and whitebait smelt Allosmerus elongatus, for their ability to serve as biological indicators of ocean conditions in the California Current large marine ecosystem (CCLME). Samples were collected during the oceanographically contrasting years of 2005 and 2006. Upwelling was severely curtailed in the spring and early summer of 2005, leading to delayed biological productivity, whereas upwelling was relatively normal in spring 2006. Principal components analysis described 78% of the variance within the …

Growth, Larval Development And Metamorphosis In Small-Mouthed Salamanders (Ambystoma Texanum), Nicholas Timothy Zellmer

Undergraduate Honors Thesis Collection

The small-mouthed salamander (Ambystoma texanum), a common species in the midwestern United States, lays its eggs in temporary ponds during mid to late March once they have filled with water from the melting winter snow or early spring rains. For its relative abundance in the Midwest, there is little known about the life history of this species. My research followed the growth of small-mouthed salamander larvae in ponds between hatching, which occurred in early spring (mid-April), and metamorphosis, which occurred in midsummer (early June to mid-July). The research focused on the growth, larval development, and timing of metamorphosis with special …

Microsatellite Analysis Of Two Local Fox Squirrel (Sciurus Niger) Populations, Sajeena R. Horvath

Undergraduate Honors Thesis Collection

The objective of this study was to examine the genetic diversity of two geographically separated populations of Eastem fox squirrels (Sciurus niger) within the Indianapolis metropolitan area. One population was located on Butler University's campus and the other at Eagle Creek Park, located about 6.5 miles WNW from the Butler campus. DNA was extracted from tail hair samples collected by live trapping squirrels during the summers of 2007, 2008, and 2009. I used microsatellite analysis to examine the genetic variation between the two sites. Hair samples from 66 Butler squirrels and 30 Eagle Creek squirrels were analyzed using 4 microsatellite …

Effects Of Feeding Distillers' Grains On Beef Quality, Amilton S. De Mello Jr.

Department of Animal Science: Dissertations, Theses, and Student Research

This research was conducted to understand the effects of feeding finishing diets containing distillers grains on beef quality. In the first and second studies, feeding wet distillers grains plus solubles (WDGS) increased proportions of PUFA, omega 6, omega 6/omega3, and trans fatty acids when compared to corn (P ≤ 0.05). Lipid oxidation was higher in muscles from animals fed WDGS when compared to corn (P ≤ 0.05). The third study examined the effects of feeding levels up to 50% of modified distillers grains plus solubles (MDGS) on fatty acid composition in beef. Linear increase (P ≤ 0.05) …

Influence Of Weaning Strategy On Animal Welfare In Beef Calves, Lindsey B. Krebs

Undergraduate Research Conference

Alternative strategies can affect the amount of stress that calves encounter at weaning. The strategies being evaluated in this study are fenceline, two-stage, and traditional abrupt. Fenceline weaning involves placing the calves in a pasture adjacent to their cows so they can have contact with each other through the fence. Two-stage weaning utilizes anti-suckling devices that clip into the calves noses and prevent them from suckling their cows. The calves remain with the cows for a short amount of time, then they are weaned and separated from their cows. This allows the stressors of weaning to be faced in two …

Diet Reconstruction Of Bighorn Sheep (Ovis Canadensis) Using Stable Isotopes, Joshua M. Whitaker

Theses and Dissertations

We determined the diet contributions of grasses, forbs and shrubs for three herds of bighorn sheep along the Wasatch Front, Utah using stable isotope techniques and determined the electivity values for different forage species for four herds. Forbs were generally the most common forage eaten across all herds while shrubs were the least used forage resource. The Provo Peak and Mount Nebo herds used grasses, forbs and shrubs at proportions similar to other bighorn sheep populations across the west, while the Antelope Island herd used forbs at higher levels than any other local herd. Additionally, the herd on Antelope Island …

Countermeasure Policy On The Samho Brother Benzene Ship Accident, Chin-Long Lin, Jian-Hwa Hu

Journal of Marine Science and Technology–Taiwan

On October 10th, 2005, the chemical ship SAMHO BROTHER mishap occurred in the Taiwan Strait while transporting a cargo of benzene. Explosive approach was applied but failed, causing the ship wreck sank to a depth of 70 m. Oil spill was recovered in a small amount, leaving about 50 tons of the fuel remained. On the other hand, environmental monitoring at the site of the accident revealed that almost all of the great amount of 3140 m3 benzene remains stored in the nine tanks on the ship and further actions upon such a unique event in the history are urgent. …

Your Attention Please: Increasing Ambient Noise Levels Elicits A Change In Communication Behaviour In Humpback Whales (Megaptera Novaeangliae), Rebecca A. Dunlop, Douglas H. Cato, Michael J. Noad

Bioacoustics Collection

High background noise is an important obstacle in successful signal detection and perception of an intended acoustic signal. To overcome this problem, many animals modify their acoustic signal by increasing the repetition rate, duration, amplitude or frequency range of the signal. An alternative method to ensure successful signal reception, yet to be tested in animals, involves the use of two different types of signal, where one signal type may enhance the other in periods of high background noise. Humpback whale communication signals comprise two different types: vocal signals, and surface-generated signals such as ‘breaching’ or ‘pectoral slapping’. We found that …

T10c12 Conjugated Linoleic Acid Causes Delipidation In 3t3-L1 Adipocytes And Mice, Shan Jiang

Department of Animal Science: Dissertations, Theses, and Student Research

T10c12 conjugated linoleic acid (t10c12 CLA) causes the reduction of triglyceride contents in adipocytes. T10c12 CLA’s delipidation effect is associated with decreased nutrient uptake, adipogenesis, lipogenesis, and increased energy expenditure, lipolysis, fatty acid oxidation in adipocytes. However, the molecular mechanisms of CLA’s delipidation effects are still unknown. AMP-activated protein kinase (AMPK), a central regulator of cellular energy levels, is activated by an increase in the cellular AMP:ATP ratio or various cellular stresses. We demonstrated that t10c12 CLA activated AMPK in 3T3-L1 adipocytes, leading to inhibition of anabolic biosynthesis and increase of energy expenditure. Strong activation of AMPK can induce an …

Perspectives On The Application Of Zilpaterol Hydrochloride In The United States Beef Industry, R. J. Delmore, J. M. Hodgen, B. J. Johnson

Animal Science

Zilpaterol hydrochloride (ZH) is a β-adrenergic agonist approved to be fed at a rate of 8.3 mg/kg (100% DM basis) during the final 20 to 40 d of the finishing period in beef cattle followed by a minimum 3-d withdrawal period antemortem. The Federal Drug Administration (FDA) approved label claims of increased rate of BW gain, improved feed efficiency, and increased carcass leanness. Before the release of ZH for commercial use in 2007, approximately 10 independent research trials at various universities and commercial feedlots were initiated. Articles in recent issues of the Journal of Animal Science are a result of …

Perspectives On The Application Of Zilpaterol Hydrochloride In The United States Beef Industry, Robert J. Delmore, Jr., J. M. Hodgen, B. J. Johnson

Animal Science

Zilpaterol hydrochloride (ZH) is a β-adrenergic agonist approved to be fed at a rate of 8.3 mg/kg (100% DM basis) during the final 20 to 40 d of the finishing period in beef cattle followed by a minimum 3-d withdrawal period antemortem. The Federal Drug Administration (FDA) approved label claims of increased rate of BW gain, improved feed efficiency, and increased carcass leanness. Before the release of ZH for commercial use in 2007, approximately 10 independent research trials at various universities and commercial feedlots were initiated. Articles in recent issues of the Journal of Animal Science are a result of …

The Loss Of Species: Mangrove Extinction Risk And Geographic Areas Of Global Concern, Beth A. Polidoro, Kent E. Carpenter, Lorna Collins, Norman C. Duke, Aaron M. Ellison, Joanna C. Ellison, Elizabeth J. Farnsworth, Edwino S. Fernando, Kandasamy Kathiresan, Nico E. Koedam, Suzanne R. Livingstone, Toyohiko Miyagi, Gregg E. Moore, Vien Ngoc Nam, Jin Eong Ong, Jurgenne H. Primavera, Serverino G. Salmo, Jonnell C. Sanciango, Sukristijono Sukardjo, Yamin Wang, Jean Wan Hong Yong

Biological Sciences Faculty Publications

Mangrove species are uniquely adapted to tropical and subtropical coasts, and although relatively low in number of species, mangrove forests provide at least US $1.6 billion each year in ecosystem services and support coastal livelihoods worldwide. Globally, mangrove areas are declining rapidly as they are cleared for coastal development and aquaculture and logged for timber and fuel production. Little is known about the effects of mangrove area loss on individual mangrove species and local or regional populations. To address this gap, species-specific information on global distribution, population status, life history traits, and major threats were compiled for each of the …

Multi-Host Model-Based Identification Of Armillifer Agkistrodontis (Pentastomida), A New Zoonotic Parasite From China, Shao-Hong Chen, Qin Liu, Yong-Nian Zhang, Jia-Xu Chen, Hao Li, Ying Chen, Peter Steinmann, Xiao-Nong Zhou

Harold W. Manter Laboratory of Parasitology: Library Materials

Background: Pentastomiasis is a rare parasitic infection of humans. Pentastomids are dioecious obligate parasites requiring multiple hosts to complete their life cycle. Despite their worm-like appearance, they are commonly placed into a separate sub-class of the subphylum Crustacea, phylum Arthropoda. However, their systematic position is not uncontested and historically, they have been considered as a separate phylum.

Methodology/Principal Findings: An appraisal of Armillifer agkistrodontis was performed in terms of morphology and genetic identification after its lifecycle had been established in a multi-host model, that is, mice and rats as intermediate hosts, and snakes (Agkistrodon acutus and Python molurus) …

Evaluation Of Feedlot And Carcass Performance Of Steers Fed Different Levels Of E-Corn, A Potential New Feed Product From Ethanol Plants, Corineah M. Godsey, Matt K. Luebbe, Joshua R. Benton, Galen E. Erickson, Terry J. Klopfenstein, Carlos Ibanez, Pablo Guiroy, Matthew A. Greenquist, Jeff Kazin

Nebraska Beef Cattle Reports

A pre-process fractionation produces a feed product called E-corn, which is low in fat and contains heat-treated starch. E-corn replaced dry rolled corn at 0, 20, 40, or 60% (DM basis) in finishing diets containing either 30% wet distillers grains plus solubles (WDGS) or 30% wet corn gluten feed (WCGF). E-corn level x byproduct type interactions were not observed. Dry matter intake increased quadratically to E-corn inclusion level (P = 0.04), while F:G responded cubically with 20% and 60% E-corn inclusion having the lowest F:G (P = 0.02). However, when E-corn level increased from 0 to 60% of diet DM, …

Mesenchymal Stem Cells Isolated From A Novel Equine Tissue Exhibit Embryonic Molecular Markers And Adipogenic Differentiation Potential, Jane Marie Isquith, Kelly Shaw, Karen Shaw, Daniel Peterson, Matthew Burd

Animal Science

Mesenchymal stem cells (MSC) have been isolated from equine tissues, most notably adipose and bonemarrow. On the basis of ability for self-renewal, molecular marker expression and differentiation potential, we characterized MSCs isolated from a tissue not yet explored in the equine. After isolation, MSCs weremaintained in culture for 25 passages before senescence was observed. Reverse transcription-PCR revealedembryonic transcription factor Oct4 mRNA, as well as mRNA for fucosyltransferase 4 and ST3B-galactosid α 2,3 sialySSEA4, respylxanthine l ltransferase 2, two enzymes responsible for synthesis of embryonic surface markersSSEA1 and ectively. Treatment with insulin, dexamethasone, indomethacin andisobutylmethed to increased oil red O staining …

Lh Increases Insl3 Mrna Abundance In Ma-10 Leydig Cells, Mary Elizabeth Strong, Matthew Anderson Burd, Daniel Gunnar Peterson

Animal Science

Leydig cells produce testosterone in response to luteinizing hormone (LH) via the cyclic adenosine monophosphate (cAMP)/protein kinase A pathway. Additionally, these cells are responsible for producing insulin-like peptide 3 (INSL3), a peptide hormone that is essential for testicular descent. The insl3 promoterin Leydig cells can be activated by cAMP through the transcription factor Nur77. While the mechanism of LH action on testosterone production is well characterized, the effect of LH on insl3 abundance has yet to be shown directly. MA-10 Leydig cells were treated with either 0 and 100 ng/mL LH for 3 and 6 hours in a 2x2 factorial …

Ua77/1 Wku Spirit, Wku Alumni Relations

WKU Administration Documents

Magazine created for the alumni of Western Kentucky University. In this issue:

• WKU Student Teaching Benefits from International Flavor
• The Horse in Kentucky
• Hilltopper Completes DNA Sequence of Virus
• Willie Taggart Takes the Helm

Comparison Of The Effects Of Growth Hormone On Mac-T Cells And Primary Mammary Cells, Lisa Mcdonnell, Tasha Johnson, Daniel G. Peterson

Animal Science

The ability of growth hormone (GH) to increase milk yield through somatomedins is well characterized,however recent studies utilizing mammary epithelial cell (MEC) lines have indicated a possible direct effecton MEC. In the mammary alveolar cell-T (MAC-T) line, relative abundance of mRNA for two milk protein genes, α-lactalbumin and αS1-casein, and GH receptor was increased by GH. MAC-T cells were compared tobovine mammary tissue explants and primary MEC isolated from raw milk through culture in classicallactation medium (dexamethasone, insulin, prolactin) with 0 or 10 ng/mL GH. Explant and primary cellmRNA abundance showed a similar pattern to MAC-T indicating that the effects …

Coastal And Marine Resource Condition Monitoring – Scoping Project, Brett A. Human

Fisheries Research Reports

This pilot study investigated potential resource condition indicators, remote sensing, and ground truthing methodologies, for their use in long term monitoring of mangroves and intertidal mudflats in the Pilbara and Kimberley.