Microbiology Commons^™

Isolation And Preliminary Characterization Of Bacteriophage Φμ-4, Fred Shafia, T.L. Thompson

Papers in Microbiology

Isolation and preliminary characterization of bacteriophage Φμ-4. J. Bacteriol. 87:999-1002. 1964.-Bacteriophage Φμ-4 was isolated from lysogenic Bacillus stearothermophilus NU strain 4, and was propagated in strain 10 of the same species. The phage was extremely host-specific. One of the 23 strains of thermophiles screened for susceptibility supported phage replication. Plaque-forming efficiency of Φμ-4 depended on the agar medium employed and the temperature of incubation. Generally, media which resulted in restricted growth of the host cells on agar plates were most satisfactory for plaque formation. A latent period of 35 min was terminated in cell lysis and release of about 175 …

Induced Fruiting In Myxobacteria, Walter Fluegel

Journal of the Minnesota Academy of Science

The fruiting myxobacteria and the cellular slime molds (Acrasiales) have many superficial resemblances with respect to fruiting behavior. Cells in the swarm are triggered by chemical substances to aggregate and form fruiting bodies (Bonner, 1947; Fluegel, 1963a). A chief difference between the two with respect to fruiting behavior, is that the myxoamoeba are free cells whereas the myxobacters are enmeshed in slime threads (Fluegel, 1963b). However, it is tacitly assumed that if parallel studies be undertaken in myxobacteria as has been done with the slime molds, the cells must be grown dispersed. Most isolates of myxobacteria do not grow dispersed; …

Plant Diseases : Angular Leaf Spot Of Cucurbits, O M. Goss

Journal of the Department of Agriculture, Western Australia, Series 4

ANGULAR leaf spot caused by the bactterium Pseudomonas lachrymans (E.F.Sm. & Bryan) Carsner, is common on cucumbers and gherkins grown late in the season.

The same disease has been recorded on rockmelons, watermelons and pumpkins but rarely occurs on these hosts in Western Australia.

The Immunological Response Of A Freshwater Fish To Bacteria Of Marine Origin, Robert S. Pekarek

Master's Theses

No abstract provided.

The Effects Of Herbicides, Fungicides, And Insecticides On Bacteria Of Marine Origin, Kenneth William Pierini

Master's Theses

No abstract provided.

Life History Of A Haemogregarine, Hepatozoon Sp. In The Chuckwalla: Sauromalus, John E. Lewis

Loma Linda University Electronic Theses, Dissertations & Projects

[Abstract Not Included]

Evaluation Of The Photometric Assay Method To Quantitate The Bactericidal Action Of Normal And Immune Rabbit Sera On Salmonella Pullorum, Alice Margaret Rahn

Loma Linda University Electronic Theses, Dissertations & Projects

The bactericidal antibody test depends on the presence of sufficient complement, magnesium ions, and specific antibody to produce killing of certain gram negative bacilli. This study was undertaken to demonstrate whether or not Salmonella pullorum is an organism which is susceptible to this killing action.

Different amounts of test serum containing antibody, constant amounts of complement and magnesium saline diluent were placed together and held in a 37°C water bath for a one hour reaction time. During this interval, the antibody-complement system was given an opportunity to kill the susceptible bacteria. Then, a constant volume of nutrient broth was added …

A Study Of The Coprophilous Ascomycetes Of Utah, David L. Hanks

Theses and Dissertations

Specimens of animal dung were collected from various areas of the state of Utah. These were cultured by placing a few small pieces in a culture dish over moistened sphagnum moss and filter paper. The cultures were observed periodically and specimens were studied as they matured upon the substratum. A total of eighty-four species representing three orders and fifteen genera are reported. Of these, nine species have not previously been described. Included is one species, Tripterospora erostrata, from the order Plectascales of the Series Plectomyceteae. Cited from the order Sphaeriales of the Series Pyrenomyceteae are forty-nine species as follow: Coniochaeta, …

Back Flushing On Milking Machine Teat Cups, G W R Scott, K. Needham

Journal of the Department of Agriculture, Western Australia, Series 4

THE possibility of replacing the dipping of teat cups between cows with a system of back flushing with water has been investigated for some time by different workers.

Absence Of Pasteurella Pestis In The Wildlife Of Arkansas, R. Reece Corey, Leo J. Paulissen, Delbert Swartz

Journal of the Arkansas Academy of Science

No abstract provided.

Chemical Sterilisers In The Dairy Industry, D N. Tulloch, N. Ingleton

Journal of the Department of Agriculture, Western Australia, Series 4

There is concern in the cheese industry that residues from new chemical sterilisers used by dairy farmers could affect cheese making processes.

The sterilisers could also affect tests for antibiotic residues in milk. The responsibility of seeing that chemical sterilisers do not enter the milk belongs to the dairy farmers.

They should follow the manufacturers' recommendations and use the chemicals with care.

Variations In Cream Tests, J F. Brennan

Journal of the Department of Agriculture, Western Australia, Series 4

DURING the year dairy farmers are often concerned about variations in the butterfat test of cream they supply to the butter factory.

Why do these tests vary? Is the variation the fault of the farmer or the butter factory?

Further Studies On An Antibiotic Substance Produced By Rhizopus Nigricans Ehrenberg, Kenneth D. Mace, Delbert Swartz

Journal of the Arkansas Academy of Science

No abstract provided.

The Effect Of Infection With Polyoma Virus On The Protein, Rna, And Dna Of Mouse Embryo Cell Cultures, Lawrence E. Mcconnehey

Loma Linda University Electronic Theses, Dissertations & Projects

In four separate experiments mouse embryo cell monolayers infected with polyoma virus and uninfected control mouse embryo cells were maintained in Eagle’s Minimum Essential Medium for periods of time ranging from 0 to 168 hours. The cell layers were removed from the glass by trypsinization. Samples of the cells were counted in a Coulter Counter and the remainder of the cells frozen after centrifugation, in a button. Later, the buttons were thawed and protein, RNA, and DNA analyses were performed on each sample. The micrograms per million cells of protein. RNA, and DNA were determined for each sample and plotted …

Quantitative Evaluation Of Streptococcal Antibodies By A Serum Inhibition Fluorescent Antibody Tube Method, Charles Glenn Nichols

Loma Linda University Electronic Theses, Dissertations & Projects

The development and description of a quantitative method of evaluating streptococcal antibodies by inhibition of fluorescent staining is reported. Suspensions of Streptococcus pyogenes group A, treated with methyl alcohol to reduce auto-fluorescence, were used as antigen. The degree of inhibition of uptake of fluorescent labeled antibody produced by the presence of antibodies in the immune test sera was measured after elution of the labeled antibody. Comparisons were made with controls using normal sera containing no inhibiting antibodies. A method of reporting the results in terms of titer is described.

Streptococcal antibodies were demonstrated quantitatively by this inhibition test in sera …

Some Characteristics Of A Purified Heat-Stable Aldolase, P.J. Thompson, T.L. Thompson

Papers in Microbiology

THOMPSON, P. J. (University of Nebraska, Lincoln) AND T. L. THOMPSON. Some characteristics of a purified heat-stable aldolase. J. Bacteriol. 84:694-700. 1962-Aldolase from a thermophilic strain of bacteria was obtained in a state of high purity. Heat studies of purified aldolases from cells cultivated at 45 and 65 C showed them equally stable at 70 C for 1 hr. Metal-ion and chelate studies indicated that thermal aldolase is metal ion-independent. Carboxypeptidase did not alter activity or specificity. The enzyme was specific for fructose- 1, 6-diphosphate. Hydrazine was found inhibitory in the assay procedure. The inhibition was independent of pH over …

Light Lands In Western Australia. 3. Microbiological Problems In The Establishment Of Legumes On Light Lands, C A. Parker

Journal of the Department of Agriculture, Western Australia, Series 4

EXPERIENCE has shown that the establishment of leguminous pastures is essential for successful economic development of Western Australian light lands.

Subterranean clover is the preferred legume in most light land areas, with lupins holding an important place in the more northerly districts, and on the deeper sands.

Pullorum Tested Poultry Flocks : 1962, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

THIS year's pullorum tests of poultry flocks have revealed the lowest incidence of reactors since the tests began.

Results are recorded below.

Mycotic Dermatitis (Lumpy Wool) And Fleece Rot Of Sheep, J Shilkin

Journal of the Department of Agriculture, Western Australia, Series 4

Matted and sometimes discoloured specimens of wool are often received at the Department of Agriculture during seasons of heavy rainfall.

Two similar but quite distinct diseases are recognised as having caused this condition.

They are mycotic dermatitis (lumpy wool) and fleece rot. Both conditions damage the fleece and render affected sheep susceptible to blowfly strike.

Malic Dehydrogenase Activity At 101 C Under Hydrostatic Pressure, Richard Y. Morita, Roger D. Haight

Papers in Microbiology

MORITA, RICHARD Y. (University of Nebraska, Lincoln) AND ROGER D. HAIGHT. Malic dehydrogenase activitv at 101 C under hydrostatic pressure. J. Bacteriol. 83:1341-1346. 1962.-No malic dehydrogenase activity was found to occur at 101 C at various hydrostatic pressures from 1 to 700 atm. However, activity was demonstrated with hydrostatic pressures above 700 atm, with optimal activity at 1,300 atm at the same temperature. Explanation of the data is based upon thermal denaturation of the enzyme, which involves a molecular volume increase of the enzyme. The molecular volume increase is counteracted by hydrostatic pressure. Pressures above 700 atm to 1,500 atm …

Detergents For The Dairy Farm, H Maslin

Journal of the Department of Agriculture, Western Australia, Series 4

CLEANLINESS in both the physical and bacteriological sense is essential to the production of high quality dairy products, and high quality in these products is essential to both the health of the community and the successful and economic operation of the industry.

Detergents have an important place in maintaining dairy hygiene—but they must be properly used if they are to be really effective.

In this article Dairy Instructor H. Maslin, M.D.D., describes the qualities of a good dairy detergent and explains how it should be fitted into the regular cleaning routine.

Interaction Between The Parameters Of Hydrostatic Pressure And Temperature On Aspartase Of Escherichia Coli, Roger D. Haight, Richard Y. Morita

Papers in Microbiology

HAIGHT, ROGER D. (University of Nebraska, Lincoln) AND RICHARD Y. MORITA. Interaction between the parameters of hydrostatic pressure and temperature on aspartase of Escherichia coli. J. Bacteriol. 83:112-120. 1962.-The data obtained from studies of an aspartase preparation and aspartase in cells of Escherichia coli indicate that there is an interaction between the parameters of hydrostatic pressure and temperature. Pressure was found to decrease aspartase activity at 45 C and lower in vitro and below 53 C in vivo, thereby indicating that when the enzymesubstrate complex is formed there is an increase in molecular volume which is counteracted by pressure. Above …

Investigation In The Physiology Of The Spore Formation Of Pseudoples Trifolii (Rostr.) And Petr., Emory S. Crosby

Masters Theses & Specialist Projects

Plant pathologists have worked to some extent for many years in an attempt to describe the complete physiology of the organism responsible for pepper spot of clover, particularly as found on white clover (Trifolium repens, L.). These works have not been extensive or concentrated and at the present time, no definite conclusions have been reached.

The causal agent of pepper spot of clover is Pseudoples trifolii (Rostr.).

Pseudoples trifolii usually is found parasitizing clover in the early spring and continues throughout the spring until the temperature exceeds thirty degrees centigrade. Elliott established that Pseudoples trifolii will not produce infectious spores …

A Comparative Study Of The Growth Of Polyoma Virus In Two Secondary Cell Lines: Mouse Lymphoma And Normal Mouse Fibroblasts, Rheeta M. Stecker

Loma Linda University Electronic Theses, Dissertations & Projects

In comparing the growth of polyoma virus in secondary cell cultures of a mouse lymphoma (P388D₁, a known polyoma-supporting strain) and normal mouse fibroblasts (Earle’s L cells), the following observations have been made.

The L cell is capable of supporting the growth of the polyoma virus with reproduction and release of infectious particles. Viruses appear in the fluid from time to time over a period of several weeks. This probably represents a carrier state of the virus in L cells. Many of the L cells in infected cultures are not killed by the virus infection.

Virus particles released …

A Study Of The Bacteriological Effectiveness Of Oral Thermometer Procedures In Three Selected Hospitals, Esther Mae Rose

Loma Linda University Electronic Theses, Dissertations & Projects

Because pathogens such as streptococcus and staphylococcus have been transferred in many hospitals, the source of the infections is of concern to doctors, nurses, and other medical personnel. As shown by the review of literature, good techniques are necessary to stop the spread of disease.

It was the purpose of this study to investigate one facet of patient safety by: (1) the culture of thermometers ready for use in three selected hospitals; (2) to determine the effectiveness of the Group, Individual, and Central Supply methods of disinfection currently practiced in the three selected hospitals.

The method for the experiment of …

Sclerotinia Rot Of Vegetables, S C. Chambers, M. Hardie

Journal of the Department of Agriculture, Western Australia, Series 4

SCLEROTINIA ROT, caused by the fungus Sclerotinia sclerotiorum, is now one of the most serious vegetable diseases in metropolitan market gardens.

Over the past decade it has been steadily increasing in prevalence and if present trends continue, many growers may be forced to abandon certain crops.

Although most vegetables are susceptible, the greatest losses are occurring in the autumn planting of beans, cauliflowers and lettuce and in the production of cauliflower seed.

Cleansers For The Milking Machine, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

WITH the opening of the flush season dairy farmers should make sure milking machines are working at maximum efficiency.

Machines not working efficiently can cause production losses and damage to cows as well as wasting farmers' time.

Is Your Water Supply Safe?, D C. Mickle

Journal of the Department of Agriculture, Western Australia, Series 4

THERE can be no doubt in anyone's mind that the need for a clean, safe and adequate water supply is essential for the production of high quality milk and cream.

It naturally follows, that, even though every possible care has been taken in an endeavour to produce clean, germ-free milk and cream, contamination by bad water can render these efforts void.

Common Bean Mosaic, W P. Cass Smith

Journal of the Department of Agriculture, Western Australia, Series 4

COMMON bean mosaic is a widespread disease which attacks both dwarf and pole bean varieties.

The disease is caused by an infective principle or virus which spreads throughout the sap of affected plants, and causes both delayed maturity and drastic reduction in yield.

Circadian Organization And Microbiology: Variance Spectra And A Periodogram On Behavior Of Escherichia Coli Growing In Fluid Culture, Franz Halberg, Robert L. Conner

Journal of the Minnesota Academy of Science

Over three decades ago Rogers and Greenbank (1) published observations on intermittent growth in a bacterial culture. These interesting data are analyzed further in this report by computational procedures ( 2-8) designed for detecting and evaluating the significance of nearly periodic phenomena. By such methods, circadian (about 24-hour) periodicity in a culture of E. coli is unmasked as a significant component of the recorded changes. These results extend the already broad scope of circadian temporal organization in microbiology.