Microbiology Commons^™

Differences In Survivorship, Development Rate And Fertility Between The Long-Winged And Wingless Morphs Of The Waterstrider, Limnoporus Canaliculatus, Anthony J. Zera

Anthony Zera Publications

Species of waterstriders (Gerridae: Hemiptera) exhibit a remarkable diversity in degree of winglessness (Vepsalainen, 1978; Calabrese, 1980; Zera, 1981). Some species consist exclusively of fully-winged individuals, while other species are composed almost exclusively of wingless individuals; many species exhibit the intermediate case of wing polymorphism and consist of various proportions of fully-winged, short-winged and/or wingless morphs. Wing-polymorphic species often exhibit dramatic spatial and/or temporal variation in morph ratios, both among populations of the same species and among species. Because of this diversity, waterstriders, especially wing polymorphic species, are ideal candidates for the study of the evolution of winglessness (or conversely, …

A Bacteriological And Chemical Analysis Of Nonpoint Source Pollution In A Karst Aquifer Bowling Green, Kentucky, Wayne Green

Masters Theses & Specialist Projects

Monthly water samples collected from four sites in the Lost River Groundwater Basin, a shallow karst aquifer in the Bowling Green-Warren County area of Kentucky, represented samples from sites receiving conduit and diffuse flow. All sites were severely contaminated with bacteria, and on some occasions the surface water criteria for some heavy metals were exceeded.

Of the total 334 bacterial colonies identified 92.1% were verified as Escherichia coli by the API20E system. Acinetobacter calcoaceticus var. anitratum accounted for 2.10% of colonies; Citrobacter freundii for 0.30% Klebsiella pneumoniae for 0.90%; Klebsiella oxytoca, 0.90%; Citrobacter amalonaticus 0.30%; Enterobacter cloacae, 1.20%; …

Seasonal And Spatial Heterogeneity In The Limnetic Zooplankton Community Of Lake Mead, Gene Robert Wilde

Publications (WR)

Zooplankton samples collected from throughout Lake Mead, in 1981-1982, demonstrate the presence of a statistically significant seasonal and spatial heterogeneity in zooplankton densities. Seasonally, the major zooplankton groups were most abundant in the spring and fall, coincident with maxima in chlorophyll-a concentrations. Successions among the various rotifers, cladocerans and copepods present in the reservoir were influenced by food availability, diapause, predation by planktivorous fish and, possibly, water temperatures. Spatial heterogeneity in zooplankton densities was unrelated to water temperature, pH, conductivity and dissolved oxygen concentrations, but was related to the abundance of phytoplankton (chlorophyll-a concentrations) and fish. Statistical analyses indicate that …

Effects Of Folic Acid Or Zinc Malnutrition On Rotaviral Infection In A Murine Model, John Douglas Morrey

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The influences of dietary deficiencies of folic acid or zinc on rotaviral disease infant mice were studied. Preliminary studies indicated that bovine and simian rotaviruses, but not porcine rotavirus, caused diarrhea in infant mice. Bovine and porcine rotaviruses were not, however, sufficiently infectious to replace murine rotavirus in studies utilizing the murine model. It was also determined that murine rotavirus purified by a cesium chloride gradient was highly infectious and useful for subsequent studies on nutritional influences of rotaviral disease. In dietary studies, female Swiss Webster mice were fed diets containing deficient, moderately deficient, or adequate levels of folic acid …

The Fungal Ecology Of The Activated Sludge Process, Douglas William Jaques

Masters Theses

Examination of wastewater secondary influent and activated sludge reveal that bacteria and fungi may exist in a negative correlation. Activated sludge may support a resident population of microfungi. All fungi isolated from secondary influent and activated sludge belong to the form-class Deuteromycetes. The density of geofungi in activated sludge may exceed that of bacterial chemolithotrophs and rival that of bacterial heterotrophs.

Purification And Properties Of A Mannitol-Binding Protein From Pseudomonas Aeruginosa, Gregory V. Plano

Masters Theses

Mannitol-binding protein from Pseudomonas aeruginosa strain PAO has been purified 36-fold from magnesium chloride cold-shock supernatants. Sephadex G-100 gel filtration and carboxymethyl Sephadex ion exchange chromotography were utilized in the purification scheme. The position of the mannitol-binding protein band was identified on sodium dodeyl sulfate-polyacrylamide gels. The mannitol-binding protein has an apparent molecular weight of 45,000 as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Bioassay Of Solubilized Bacillus Thuringiensis Var. Israelensis Crystals By Attachment To Latex Beads, Danny J. Schnell, Mary Ann Pfannenstiel, Kenneth W. Nickerson

Papers in Microbiology

Solubilized crystals of Bacillus thuringiensis var. israelensis were 7,000 times less toxic to Aedes aegypti larvae than intact crystals, presumably because mosquito larvae are filter feeders and selectively concentrate particles while excluding water and soluble molecules. A procedure is described whereby soluble toxins are adsorbed to 0.8- micrometer latex beads, with retention of toxicity. The latex bead assay should make it possible to analyze the structure and mode of action of the mosquito toxin.

Prevalence Of Extreme Detergent Resistance Among The Enterobacteriaceae, Vance Kramer, Kenneth Nickerson, Nancy V. Hamlett, Caroline O'Hara

Kenneth Nickerson Papers

The detergent-resistance properties of 208 independent isolates of the Enterobacteriaceae have been examined. Of these bacterial strains, 200 were able to grow in the presence of ≥5% sodium dodecyl sulfate, including all members of the Klebsielleae tribe. This resistance does not appear to be plasmid encoded. It is proposed that detergent-resistant organisms he termed saponotolerant or saponophilic, by analogy with other microorganisms occupying harsh ecological niches. In contrast to their prevalent resistance to anionic detergents, not one of the 208 strains tested was found to grow in the presence of three different cationic detergents. This sensitivity to cationic detergents may …

Las Vegas Wash Multispectral Scanner Survey, T. H. Mace, M. V. Olsen, Environmental Protection Agency

Publications (WR)

At the request of the U.S. Bureau of Reclamation, Boulder City, Nevada, the U.S. Environmental Protection Agency's Environmental Monitoring Systems Laboratory at Las Vegas collected multispectral scanner imagery of Las Vegas Wash on October 1, 1982. A combined maximum likelihood classification and editing procedure was used to classify the multispectral scanner imagery into 12 categories of land cover. The classification identified four categories of marsh vegetation, one category of riparian, two categories of mixed scrub, and two desert categories. Turbid water and cultivated land formed an "other" category. Area tabulations were formed by georeferencing the classification to the Universal Transverse …

Activation Of A Translocated Human C-Myc Gene By An Enhancer In The Immunoglobulin Heavy-Chain Locus, Adrian C. Hayday, Stephen D. Gillies, Haruo Saito, Charles Wood, Klas Wiman, William S. Hayward, Susumu Tonegawa

Nebraska Center for Virology: Faculty Publications

A tissue-specific transcriptional enhancer element that is associated with the human immunoglobulin heavy-chain locus is defined. In a non-Hodgkin’s lymphoma that contains a translocated c-myc gene this enhancer is retained on the 14q⁺ chromosome and occurs within sequences shown to activate previously cryptic promoters of the c-myc gene.

Extracellular Enzyme Production By Rhizopus And Mucor Species On Solid Media, D Thompson, Broderick Eribo

Department of Biology Faculty Publications

Solid media were employed to determine the presence and absence of extracellular enzyme production by two genera of fruit-rot fungi, Rhizopus and Mucor. The results of this investigation revealed that phosphatase was released into the cultural medium by all the fungi examined; however, only R. oryzae, R. tritici, M. mucedo, and M. piriformis showed the possibility of being high producers of the enzyme. Protease, urease, ribonuclease, pectate lyase, and polygalacturonase, at varying levels of activity, were detected, in the majority of the fungi, in the cultural medium.

Dispelling Some Myths About 'Dermo', J R. Edwards

Journal of the Department of Agriculture, Western Australia, Series 4

There are a lot of misconceptions and myths about dermatophilosis, a disease which can kill young lambs, affect wool quality, make shearing difficult and make sheep susceptible to fly strike.

One misconception is perpetuated by the incorrect use of the name mycotic dermatitis, which implies that a fungus causes the disease. This is not so.

The disease is caused by the bacteria Dermatophilus congolensis, which attacks the sheep's skin and eventually forms a lesion or scab in the wool, and so is better called dermatophilosis, 'dermo' or 'lumpy wool'.

Veterinary Epidemiologist at the Albany Regional Office, J.R. Edwards, discusses …

New Developments In Footrot Control, R B. Richards, L. J. Depiazzi, R. V. R. Gwynn

Journal of the Department of Agriculture, Western Australia, Series 4

The eradication policy for footrot in sheep adopted by the Western Australian Department of Agriculture has produced an overall decline in the number of properties with the disease.

Our knowledge of the disease has improved substantially as a result of the research conducted at the Slabany Regional Veterinary Laboratory in recent years. Laboratory tests now sssist stock inspectors and veterinary officers to distinguish between different forms of footrot and to quarantine and eradicate accordingly.

The recent discovery of more effective foot-bathing solutions by officers of the Victorian Department of Agriculture will greatly assist further eradication procedures.

Western Australia's sheep industry …

Characterization Of The Lipopolysaccharides (Lps) From Four Transposon Generated Symbiotic Mutants Of Rhizobium Trifolii, Robert G. Shatters Jr.

Masters Theses

The LPSs from four symbiotic mutants of R. trifolii were isolated and characterized. The mutants were obtained from Dr. Barry Rolfe of The Australian National University. Three mutants have Tn5 insertions in the "nod" regions of the symbiotic plasmid. The fourth has a 30 kb deletion. This deletion includes the majority of the "nod" regions. All mutants fail to nodulate clover and fail to induce markedly curled root hairs, i.e. they are hac-nod-. The LPSs were purified as described by Carlson et al. 1978.⁷ The polysaccharide portion of the LPSs was purified by treating the LPSs in 1% HAc …

Comparison Of Surface Polysaccharides Of R. Leguminosarum Anu57 (Exo-1), R. Leg. Anu57(Pbr1an) (Nod+, Nif-), And R. Leg. Anu57(Pjb5j1) (Nod+, Nif-), Frank G. Hustmyer

Masters Theses

Extracellular polysaccharides (EPS) and lipopolysaccharides (LPS) are suspected to be involved in the legume/Rhizobium symbiotic process. Polysaccharide compositions from the EPS, LPS, capsular polysaccharide (CPS), and small polysaccharide (SmPS) were compared between Rhizobium leguminosarum ANU57 (parent, Exo^-1) and two mutants R. leg. ANU57(pBR1AN) (nod⁺, nif^-) and R. leg. ANU57(pJB5J1) (nod⁺, nif^-) both containing a Sym (symbiotic) plasmid insertion. R. leg. ANU57(pBR1AN) and R. leg. ANU57(pJB5J1) are nod⁺ on clover and peas, respectively. The nod⁺ mutants produce larger amounts of EPS and decreased amounts of LPS compared to …

Development Of A Dna Probe For The Rapid Detection Of Cytomegalovirus, Nell Lurain

Master's Theses

No abstract provided.

The Isolation And Identification Of Legionella Species From Environmental Sites In Rowan County, Kentucky, Sabo E. Yakubu

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science by Sabo E. Yakubu on December 17, 1983.

Toxicity Of Bacillus Thuringiensis Var. Israelensis Crystals To Aedes Aegypti Larvae: Carbonate Reversal, Danny J. Schnell, Kenneth Nickerson

Papers in Microbiology

The toxicity of purified Bacillus thuringiensis var. israelensis crystals to larvae of Aedes aegypti could be reversed 100-fold by levels of K₂CO₃ as low as 0.15%.

Genetic And Environmental Determinants Of Wing Polymorphism In The Waterstrider Limnoporus Canaliculatus, Anthony J. Zera, David J. Innes, Margaret E. Saks

Anthony Zera Publications

Wing polymorphism commonly occurs in many insects, especially species of Orthoptera, Coleoptera, Homoptera, and Hemiptera (Harrison, 1980). The polymorphism consists of discrete differences in wing length with morphs exhibiting fully developed, reduced, or totally absent wings. In addition to differences in wing length, morphs often differ in a number of other characteristics such as degree of flight muscle development, duration of nymphal development, time to first reproduction, fertility and diapause (Anderson, 1973; Vepsäläinen, 1978; Harrison, 1980).

Wing polymorphism is an attractive system for investigating the evolution of dispersal in natural populations (Vepsäläinen, 1978; Denno and Grissell, 1979; Harrison, 1980). A …

Two Transcription Products Of The Vesicular Stomatitis Virus Genome May Control L-Cell Protein Synthesis, David D. Dunigan, Jean M. Lucas-Lenard

Nebraska Center for Virology: Faculty Publications

When mouse L-cells are infected with vesicular stomatitis virus, there is a decrease in the rate of protein synthesis ranging from 20 to 85% of that in mock-infected cells. Vesicular stomatitis virus, irradiated with increasing doses of UV light, eventually loses this capacity to inhibit protein synthesis. The UV inactivation curve was biphasic, suggesting that transcription of two regions of the viral genome is necessary for the virus to become inactivated in this capacity. The first transcription product corresponded to about 373 nucleotides, and the second corresponded to about 42 nucleotides. Inhibition of transcription of the larger product by irradiating …

Microbiology And Immunology, John W. Vennes

UND Departmental Histories

This departmental history was written on the occasion of the UND Centennial in 1983.

Effects On Iron And Cobalt On Methane Production From Dairy Cattle Manure, Mark E. Himes

Retrospective Theses and Dissertations

The effects of iron and cobalt on methanogenesis from dairy cattle manure were studied. Four-liter digestors with 3-liter working volumes were charged daily with dairy manure (4.5% W/V volatile solids) to achieve a 3-day retention time. Digestors were incubated at 37°C and pH 7.0 on a rotary shaker. Duplicate digestors were maintained under the following parameters: controls (no ions added), Co⁺⁺ at 4.958 mg/liter, Fe⁺⁺ at 20.64 mg/liter and Fe⁺⁺ and Co⁺⁺ at above stated final digestor concentrations.

Significantly higher production of biogas and methane occurred with the addition of iron (p = .05). Iron-amended digestors …

Raw Water Quality In The New Charleston, Illinois Pump Storage Reservoir, Kevin G. Lookis

Masters Theses

Charleston, Illinois, a city located in east-central Illinois, recently implemented a new concept in a raw water system. Due to storage capacity-sedimentation problems in the old Lake Charleston-Embarras River raw water supply, the Charleston Pump Storage Reservoir was constructed. The new system involved building a dike separating the Lake Charleston impoundment from the Embarras River. Water to increase the storage capacity of the reservoir can now be selectively pumped into the impoundment from the river.

Raw water quality in the reservoir was monitored on a weekly basis for a full year from August, 1981 to August, 1982. The levels of …

Characterization Of Danazol Binding To Specific Cytosol Receptors In Vitro, Gail Wright Russell

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirement for the Degree of Master of Science in Biology by Gail Wright Russell on August 26, 1982.

Comparison And Correlation Of Four Methods Of Measuring Immunity To Human Cytomegalovirus, James A. Brandt

Loma Linda University Electronic Theses, Dissertations & Projects

The complement fixation test (CF) is currently the test most often used to determine the presence of antibody to human Cytomegalovirus (CMV). Several more recent techniques have now been adapted for this purpose. A comparison of CMV antibody titers was made between the CF test, a commercially available enzyme-linked immunosorbent assay (ELISA), an indirect immunofluorescent technique (IFA), and a modified indirect hemagglutination test (IHA). Forty three serum samples were tested for antibodies to CMV by each of the above procedures. The ELISA, IFA, and IHA titers were in close agreement on all samples tested. The ELISA, IFA, and IHA titers …

Cole1 Copy Number Mutants., Londa Schmidt, Joseph Inselburg

Dartmouth Scholarship

A deletion mutant of the colicin E1-derived plasmid, pDMS6642, exhibited an approximately fourfold increase in copy number. We subsequently isolated hydroxylamine-induced mutants of that plasmid that had a further increase in copy number. Analysis of them suggests that the increased copy number of pDMS6642 is associated with transcriptional readthrough from a Tn3 transposon into the region of ColE1 containing information that influences plasmid replication. The hydroxylamine mutation in one copy number mutant appeared to increase the plasmid copy number by stimulating readthrough transcription from the Tn3 transposon into the ColE1 replication control region, whereas the other hydroxylamine mutation acts by …

Mast Cell Histamine Release Induced By Portuguese Man-Of-War (Physalia) Venom, A. Lester Flowers

Loma Linda University Electronic Theses, Dissertations & Projects

Richet and Portier studied the immunization of dogs against attenuated toxic extracts of the sea anemone and showed that, when given secondary injections of sublethal amounts of native toxic extracts, the dogs experienced severe pruritus and insensitivity followed by death. They called this increased sensitivity to the toxins, anaphylaxis. Richet and Portier, and later Jacques and Schachter, and Uvnäs also studied the direct pruritic and histamine releasing effects of cnidarian (e.g. Physalia, Actinia, Cyanea), toxic extracts. While the anaphylactic effect, discovered by Richet and Portier, has been extended by numerous studies to provide the foundation of our current knowledge of …

Purification Of Poly-3-Hydroxybutyrate By Density Gradient Centrifugation In Sodium Bromide, Kenneth W. Nickerson

Papers in Microbiology

Fractionation of fully sporulated cultures of Bacillus thuringiensis by density gradient centrifugation in NaBr produced two bands which were identified as poly- β -hydroxybutyrate. This technique generated high yields of membranebound and unbound granules of exceptional purity and degree of polymerization.

Evidence For The Horizontal Acquisition Of Murine Akr Virogenes By Recent Horizontal Infection Of The Germ Line, Stephen J. O'Brien, Janet L. Moore, Malcolm A. Martin, James E. Womack

Biology Faculty Articles

Several recent reports have established the biological and molecular genetic similarity between the endogenous AKV virus of strain AKR, and an N-ecotropic endogenous virus found in the genome of feral Japanese mice, Mus musculus molossinus. The similarities are so striking as to suggest a common origin of these viruses, which are present in some, but not all, inbred mouse strains. The virogenes of AKR mice may have been acquired by either: (a) common descent of AKR (and other AKV⁺ strains) from a common ancestor of AKR and molossinus animals, or (b) horizontal germ line infection of the AKR …

Sunlight Induced Movement Of Planktonic Organisms And Their Relationships To Water Movements, John Calkins, Ed Colley, Jay Wallingford, Carol Hulsey, Kathy Lohr, Marc Bolling

KWRRI Research Reports

Previous studies have shown the planktonic organisms of various natural water bodies to be quite sensitive to the levels of solar ultraviolet radiation incident at the water surface in summer. It has been assumed that the majority of the phytoplankton are simply carried about in the mass of the water which they inhabit with little control of their position or sunlight exposure. Photosynthetic microorganisms must strike a delicate balance with regard to solar radiation; they must obtain enough visible light for adequate photosynthesis while avoiding an excessive exposure to the injurious solar UV-B radiation.

It is proposed that the circulation …