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Articles 7771 - 7800 of 8418

Full-Text Articles in Microbiology

An Improved Method For Chemical Devitellinization Of X-Gal Stained Drosophila Embryos, Amit Singh, Madhuri Kango-Singh, P. Sinha Feb 1995

An Improved Method For Chemical Devitellinization Of X-Gal Stained Drosophila Embryos, Amit Singh, Madhuri Kango-Singh, P. Sinha

Biology Faculty Publications

In Drosophila developmental biological studies, X-gal staining is commonly employed to study the spatio-temporal expression of the lacZ reporter gene in the transformed flies or their embryos. Study of the lacZ pattern in embryos often suffers from the lack of an efficient and high yieldirrg technique for devitellinization of X-gal stained embryos. Devitellinization techniques employed during antibody staining, in situ hybridization or embryonic cuticular preparations generally do not give satisfactory results when used for similar purpose in X-gal stained embryos. This results in the flaky appearance of the blue stain. We present here an improved chemical devitellinization technique which gives …

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A Novel Translational Regulation Function For The Simian Virus 40 Large-T Antigen Gene., Prithi Rajan, Sathyamagalam Swaminathan, Jiyue Zhu, Charles N. Cole Feb 1995

A Novel Translational Regulation Function For The Simian Virus 40 Large-T Antigen Gene., Prithi Rajan, Sathyamagalam Swaminathan, Jiyue Zhu, Charles N. Cole

Dartmouth Scholarship

Cells use the interferon-induced, double-stranded-RNA-dependent protein kinase PKR as a defense against virus infections. Upon activation, PKR phosphorylates and thereby inactivates the protein synthesis initiation factor eIF-2, resulting in the cessation of protein synthesis. Viruses have evolved various strategies to counteract this cellular defense. In this paper, we show that simian virus 40 (SV40) large-T antigen can antagonize the translational inhibitory effect resulting from the activation of PKR in virus-infected cells. Unlike the situation with other virus-host cell interactions, SV40 large-T antigen does not block the activation of PKR, suggesting that SV40 counteracts the cellular antiviral response mediated by PKR …

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Cyanonews (Vol. 11, No. 1, February 1995), Jeff Elhai Jan 1995

Cyanonews (Vol. 11, No. 1, February 1995), Jeff Elhai

CyanoNews

CyanoNews was a newsletter that served the cyanobacteriological community from 1985 to 2003, with content provided by readers (sort of a blog before there were blogs). The newsletter reported new findings from the lab, summaries of recent meetings (often provided by graduate students and post-docs entering the field), positions sought or available, life transitions, a compendium of recent cyanobacteria-related articles, and other items of interest to those who study cyanobacteria.

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Biotransformation Of Bile Acids, Cholesterol And Steroids. Chapter 13 In: The Ecology And Physiology Of Gastrointestinal Microbes. Vol. 1, New York, Chapman And Hall., Stephen Baron, Phillip B. Hylemon Jan 1995

Biotransformation Of Bile Acids, Cholesterol And Steroids. Chapter 13 In: The Ecology And Physiology Of Gastrointestinal Microbes. Vol. 1, New York, Chapman And Hall., Stephen Baron, Phillip B. Hylemon

Biology Faculty Scholarship

No abstract provided.

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Cyanonews (Vol. 11, No. 2, July 1995), Jeff Elhai Jan 1995

Cyanonews (Vol. 11, No. 2, July 1995), Jeff Elhai

CyanoNews

CyanoNews was a newsletter that served the cyanobacteriological community from 1985 to 2003, with content provided by readers (sort of a blog before there were blogs). The newsletter reported new findings from the lab, summaries of recent meetings (often provided by graduate students and post-docs entering the field), positions sought or available, life transitions, a compendium of recent cyanobacteria-related articles, and other items of interest to those who study cyanobacteria.

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Media Components Influence Viral Gene Expression Assays In Human Fetal Astrocyte Cultures, Micheline Mccarthy, Charles Wood, Larisa Fedoseyeva, Scott R. Whittemore Jan 1995

Media Components Influence Viral Gene Expression Assays In Human Fetal Astrocyte Cultures, Micheline Mccarthy, Charles Wood, Larisa Fedoseyeva, Scott R. Whittemore

Nebraska Center for Virology: Faculty Publications

In vitro neurovirological studies of viral infectivity or viral gene expression may be confounded by the mulHple neural cell types and/or fibrob last contamination present in early passage cultures prepared from dissociated human central nervous system (eNS) tissue. We have developed highly enriched astrocyte cultures for neurovirological study by culturing in a serum-free defined medium, 816, supplemented with basic fibroblast growth factor (FGF-2). Subculture in this medium selects against fibroblast proliferation and favors sustained proliferation of a highly enriched glial fibrillary acidic protein (GFAP)-positive cell population. These astrocytes support productive replication of cytomegalovirus (CMV) and transient expression of transfected CMVand …

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Lichen Use By Larval Leucochrysa Pavida (Neuroptera:Chrysopidae), Penni Jo Wilson Jan 1995

Lichen Use By Larval Leucochrysa Pavida (Neuroptera:Chrysopidae), Penni Jo Wilson

Masters Theses

Leucochrysa pavida is a species of green lacewing (Neuroptera:Chrysopidae) that uses lichen fragments to build a larval debris "packet." The "packet" is eventually used to form a cocoon used during pupation. Only minute lichen fragments are harvested for formation of the packets which makes identification of the actual lichens utilized difficult using morphological characteristics. For this reason, thin-layer chromatography was employed. The thin-layer chromatographic process identifies acetone-soluble lichen chemicals that are formed in lichen thalli. By comparing the chemistry of lichen communities at Tower Rock Recreation Area in Hardin County, Illinois, with the chemicals identified during thin-layer chromatography of larval …

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Seroprevalence Of Toxoplasma Gondii In Domestic Animals In Pakistan, M Zaki Jan 1995

Seroprevalence Of Toxoplasma Gondii In Domestic Animals In Pakistan, M Zaki

Department of Pathology and Laboratory Medicine

The seroprevalence of Toxoplasma gondii infections among food animals in South-West Pakistan was measured by serological examination using a commercial latex agglutination test (LAT). In all 262 blood samples, from 100 cattle, 40 sheep, 58 goats and 64 chickens, were collected from the city abattoir. Titers considered diagnostically significant (> or = 1:64) were detected in 25% of cattle and 2.5% of sheep. None of the goats and chickens presented seropositivity for anti-toxoplasma antibodies. The study suggests the need for further investigations to determine the impact of these findings on the human population.

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The Surveillance And Control Of Foodborne Diseases, Donal J. Daly Jan 1995

The Surveillance And Control Of Foodborne Diseases, Donal J. Daly

Theses

There is great variation in the extent of surveillance of foodbome disease throughout the world. Some countries contribute important epidemiological and microbiological data to the WHO Surveillance Programme for Foodbome Diseases in Europe. While the UK is a major contributor to this programme, data from Eire has been non-existent. Trends in the incidence of foodbome disease in the UK may have particular relevance to Eire given the similarity of culture. Microbiological and epidemiological data in relation to foodbome pathogens, foods most frequently incriminated in outbreaks, places where outbreaks occur, together witli the main contributory factors are discussed. The broader area …

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In Vitro Activity Of Four Fluoroquinolones On Selected Bacteria, Sheryl L. Pullen Jan 1995

In Vitro Activity Of Four Fluoroquinolones On Selected Bacteria, Sheryl L. Pullen

University of the Pacific Theses and Dissertations

In 1990-1991, in a national surveillance study, and in 1991-1992, in a followup study, both by Thornsberry et al. (1993), ciprofloxacin data from various geographical and demographical institutions were collected. Several species of bacteria have shown resistance to ciprofloxacin and norfloxacin, but the degree of resistance to these drugs has not been reported for the Stockton area. To determine the extent of this resistance, Dameron Hospital antibiograms generated from 1990 to 1994 were reviewed and compared. Results of the comparison show that susceptibility among the Gram-negative isolates, with the exception of Providencia stuartii, Acinetobacter lwoffi, and to a lesser extent …

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Hyla Albopunctata, Rafael O. De Sá Jan 1995

Hyla Albopunctata, Rafael O. De Sá

Biology Faculty Publications

Hyla albopunctata is a medium-sized species in the albopunctata group, adults range in size from 42-52 mm SVL (males average 47 mm and females 49 mm). The head is triangular in outline, always longer than wide. The snout is moderately long and acuminate in dorsal view, whereas in lateral view, it is acuminate and protrudes beyond the mandible to form an angle of 45 degrees over the mandible. The canthus rostralis is rounded and distinct, the loreal region is slightly concave, and the internarial region is slightly convex. The eyes are large, approximately twice the diameter of the tympanum. The …

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Effects Of Rearing Environment On The Somatic Diversification Of Rabbit Immunoglobulin Vdj Genes, David P. Brashinger Jan 1995

Effects Of Rearing Environment On The Somatic Diversification Of Rabbit Immunoglobulin Vdj Genes, David P. Brashinger

Master's Theses

No abstract provided.

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The Role Of Follicular Dendritic Cells In Human Immunodeficiency Virus Pathogenesis, Sonya Lynn Heath Jan 1995

The Role Of Follicular Dendritic Cells In Human Immunodeficiency Virus Pathogenesis, Sonya Lynn Heath

Theses and Dissertations

Infection with Human Immunodeficiency Virus-1(HIV) results in a disease process characterized by three stages: an acute phase characterized by viremia, a clinically latent stage with little or no detection of virus in the blood, and the last stage, AIDS, which. is characterized. by marked immunodeficiency. During clinical latency, CD4+ T cells decline over a period lasting from a few to several years. Throughout this period, HIV is found trapped on the surface of follicular dendritic cells(FDC) in the germinal centers of secondary lymphoid tissues and this is the primary site of active viral replication. We hypothesize that FDC, and the …

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A Survey Of Source And Finished Water Supplies For Giardia Cysts And Cryptosporidium, Lisa Martin Sexton Dec 1994

A Survey Of Source And Finished Water Supplies For Giardia Cysts And Cryptosporidium, Lisa Martin Sexton

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Science and Technology at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Lisa Martin Sexton on December 1, 1994.

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Transcriptional Regulation Of Insulin-Like Growth Factor : Binding Protein-4 By Protein Kinase A And Protein Kinase C Signal Transduction Pathways In Human Bone Cells, Kuk-Wha Lee Dec 1994

Transcriptional Regulation Of Insulin-Like Growth Factor : Binding Protein-4 By Protein Kinase A And Protein Kinase C Signal Transduction Pathways In Human Bone Cells, Kuk-Wha Lee

Loma Linda University Electronic Theses, Dissertations & Projects

The Insulin-like Growth Factor (IGF) system is an important local regulator of osteoblast proliferation, the primary determinant of bone formation. Six IGF Binding Proteins (IGFBPs) either inhibit or enhance IGF actions. Previous studies have shown that IGFBP-4 is an important negative regulator of osteoblast cell proliferation and that agents such as PTH which increase intracellular cAMP significantly increase expression of IGFBP-4 at the protein and mRNA levels. The underlying molecular mechanism which accounted for IGFBP-4 expression had not been determined and was the focus of my dissertation. Agents which increase intracellular cAMP rapidly increased IGFBP-4 mRNA levels. Nuclear run-off experiments …

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Correlation Between Precolonization Of Trigeminal Ganglia By Attenuated Strains Of Pseudorabies Virus And Resistance To Wild-Type Virus Latency, L. M. Schang, G. F. Kutish, Fernando A. Osorio Dec 1994

Correlation Between Precolonization Of Trigeminal Ganglia By Attenuated Strains Of Pseudorabies Virus And Resistance To Wild-Type Virus Latency, L. M. Schang, G. F. Kutish, Fernando A. Osorio

Nebraska Center for Virology: Faculty Publications

We compared the levels of latent pseudorabies virus (PRV) DNA in trigeminal ganglia (TG) of pigs after intranasal inoculation of different PRV strains by using quantitative DNA PCR. The extent of colonization attained in each case varied significantly according to the type of strain and inoculum dose, wild-type (WT) PRV being the most efficient strain in colonizing TG. When groups of pigs representing different levels of precolonization of TG with an attenuated PRV strain were challenged with WT PRV, it became evident that there is a statistically significant inverse correlation between the extent of precolonization attained by an attenuated PRV …

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Alpha Interferon-Induced Antiretroviral Activities: Restriction Of Viral Nucleic Acid Synthesis And Progeny Virion Production In Human Immunodeficiency Virus Type 1-Infected Monocytes, Lisa Baca-Regen, Nina Heinzinger, Mario Stevenson, Howard Gendelman Nov 1994

Alpha Interferon-Induced Antiretroviral Activities: Restriction Of Viral Nucleic Acid Synthesis And Progeny Virion Production In Human Immunodeficiency Virus Type 1-Infected Monocytes, Lisa Baca-Regen, Nina Heinzinger, Mario Stevenson, Howard Gendelman

Nebraska Center for Virology: Faculty Publications

Alpha interferon (IFN-a) restricts multiple steps of the human immunodeficiency virus type 1 (HIV-i) life cycle. A well-described effect of IFN-a is in the modulation of viral nucleic acid synthesis. We demonstrate that IFN-α influences HIV-1 DNA synthesis principally by reducing the production of late products of reverse transcription. The magnitude of IFN-α-induced downregulation of HIV-1 DNA and/or progeny virion production was dependent on the IFN-α concentration, the duration of cytokine administration, the multiplicity of infection, the viral strain, and the cycles of viral infection. Interestingly, reductions in viral DNAs could not fully account for the observed IFN-a-induced abrogation of …

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Characterization Of Lysosomal Membrane Proteins Of Dictyostelium Discoideum, Lesly A. Temesvari, Juan Rodriguez-Paris, John Bush, Theodore L. Steck, James Caradelli Oct 1994

Characterization Of Lysosomal Membrane Proteins Of Dictyostelium Discoideum, Lesly A. Temesvari, Juan Rodriguez-Paris, John Bush, Theodore L. Steck, James Caradelli

Publications

Highly purified lysosomes, prepared by magnetic fractionation of homogenates from Dictyostelium discoideum cells fed colloidal iron, were lysed under hypoosmotic conditions, and the membrane-associated proteins were subjected to gel electrophoresis. Thirteen major membrane polypeptides, ranging in molecular weight from 25,000 to 100,000 were identified. The isoelectric points of these proteins ranged from below 3.8 to greater than 7.0. Most of these proteins were stripped from membranes exposed to a chaotropic agent, 3,5-diodo-2-hydroxybenzoic acid lithium salt, and were therefore classified as peripheral membrane proteins. Twenty five glycoprotein species were detected by lectin blot analysis; 19 were classified as integral membrane proteins, …

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A Rab4-Like Gtpase In Dictyostelium Discoideum Colocalizes With V-H(+)-Atpases In Reticular Membranes Of The Contractile Vacuole Complex And In Lysosomes, John Bush, Kathleen Nolta, Juan Rodriguez-Paris, Nancy Kaufmann, Theresa O'Halloran, Tracy Ruscetti, Lesly A. Temesvari, Theodore Steck, James Cardelli Oct 1994

A Rab4-Like Gtpase In Dictyostelium Discoideum Colocalizes With V-H(+)-Atpases In Reticular Membranes Of The Contractile Vacuole Complex And In Lysosomes, John Bush, Kathleen Nolta, Juan Rodriguez-Paris, Nancy Kaufmann, Theresa O'Halloran, Tracy Ruscetti, Lesly A. Temesvari, Theodore Steck, James Cardelli

Publications

In the course of screening a cDNA library for ras-related Dictyostelium discoideum genes, we cloned a 0.7 kb cDNA (rabD) encoding a putative protein that was 70% identical at the amino acid level to human Rab4. Rab4 is a small M(r) GTPase, which belongs to the Ras superfamily and functions to regulate endocytosis in mammalian cells. Southern blot analysis indicated that the rabD cDNA was encoded by a single copy gene while Northern blot analysis revealed that the rabD gene was expressed at relatively constant levels during growth and differentiation. Affinity-purified antibodies were prepared against a RabD fusion protein expressed …

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Comparison Of Disulfide Contents And Solubility At Alkaline Ph Of Insecticidal And Noninsecticidal Bacillus Thuringiensis Protein Crystals, Cheng Du, Phyllis A.W. Martin, Kenneth W. Nickerson Oct 1994

Comparison Of Disulfide Contents And Solubility At Alkaline Ph Of Insecticidal And Noninsecticidal Bacillus Thuringiensis Protein Crystals, Cheng Du, Phyllis A.W. Martin, Kenneth W. Nickerson

Papers in Microbiology

We compared two insecticidal and eight noninsecticidal soil isolates of Bacillus thuringiensis with regard to the solubility of their proteinaceous crystals at alkaline pH values. The protein disulfide contents of the insecticidal and noninsecticidal crystals were equivalent. However, six of the noninsecticidal crystals were soluble only at pH values of ≥12. This lack of solubility contributed to their lack of toxicity. One crystal type which was soluble only at pH ≥12 (strain SHP 1-12) did exhibit significant toxicity to tobacco hornworm larvae when the crystals were presolubilized. In contrast, freshly prepared crystals from the highly insecticidal strain HD-1 were solubilized …

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Characterization Of An Antiviral Agent Based On Nonionic Surfactants And It's Effects On Human Dermal Fibroblasts, Ji Young Li Oct 1994

Characterization Of An Antiviral Agent Based On Nonionic Surfactants And It's Effects On Human Dermal Fibroblasts, Ji Young Li

Biological Sciences Theses & Dissertations

The AIDS ·pandemic has directed various research endeavors towards finding an appropriate method for eliminating all potentially infectious material within bone allografts before implantation into a recipient. To that effect, Panavirocide was conceived by Medicine and Applied Science·s, Inc. This compound, in which three nonionic surfactants serve as the active agents, has been shown to inactivate HIV-1 particles within blood and blood products. Because Panavirocide has never been used with allografts, the purpose of the present research was to characterize the nonionic surfactants for the putative alteration of the formulation by determining the critical micelle concentration values and to determine …

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Effects Of Natural Sequence Variation On Recognition By Monoclonal Antibodies Neutralize Simian Immunodeficiency Virus Infectivity, Weon Sang Choi, Catherine Collignon, Clotilde Thiriart, Dawn P. Wooley, E. J. Scott, Karen A. Kent, Ronald C. Desrosiers Sep 1994

Effects Of Natural Sequence Variation On Recognition By Monoclonal Antibodies Neutralize Simian Immunodeficiency Virus Infectivity, Weon Sang Choi, Catherine Collignon, Clotilde Thiriart, Dawn P. Wooley, E. J. Scott, Karen A. Kent, Ronald C. Desrosiers

Neuroscience, Cell Biology & Physiology Faculty Publications

The determinants of immune recognition by five monoclonal antibodies (KK5, KK9, KK17, Senv7.1, and Senv101.1) that neutralize simian immunodeficiency virus infectivity were analyzed. These five neutralizing monoclonal antibodies were generated to native SIVmac251 envelope glycoprotein expressed by a vaccinia virus recombinant vector. All five recognize conformational or discontinuous epitopes and require native antigen for optimal recognition. These monoclonal antibodies also recognize SIVmac239 gp120, but they do not recognize gp120 of two natural variants of SIVmac239, 1-12 and 8-22, which evolved during the course of persistent infection in vivo (D.P.W. Burns and R.C. Desrosiers, J. Virol. 65:1843-1854, 1991). Recombinant viruses which …

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Peculiar Histopathological Features Of Giardiasis In Distal Duodenal Biopsies, Z Abbas, A A. Qureshi, H Sheikh, S M. Jafri, A H. Khan Sep 1994

Peculiar Histopathological Features Of Giardiasis In Distal Duodenal Biopsies, Z Abbas, A A. Qureshi, H Sheikh, S M. Jafri, A H. Khan

Department of Pathology and Laboratory Medicine

Histological changes in 20 Giardia positive duodenal biopsies (Group A) were compared with 50, Giardia negative duodenal biopsies (Group B), taken during the same period. Stool examinations in Group B were negative for Giardia. Surface epithelium, villous and crypt architecture and cellular infiltrates were examined and compared between the groups. Atrophic changes in the villi were more common in Group A as compared to B(P < 0.0001). Intraepithelial neutrophil infiltration (P < 0.001), infiltration of the lamina propria with plasma cells (P < 0.5), and presence of eosinophils in the lamina propria (P < 0.001) were significant findings in group A. Some of the changes were related to the density of Giardia colonization e.g., the goblet cell depletion (P < 0.05) and the density of plasma cell infiltration in lamina propria (P < 0.01). Erosions and ulcerations were less commonly seen in group A. Thus we conclude that giardiasis manifests its peculiar features in the distal duodenal mucosa and a biopsy of this region is an important diagnostic tool for detection of this disease.

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A Lion Lentivirus Related To Feline Immunodeficiency Virus: Epidemiologic And Phylogenetic Aspects, Eric W. Brown, Naoya Yuhki, Craig Packer, Stephen J. O'Brien Sep 1994

A Lion Lentivirus Related To Feline Immunodeficiency Virus: Epidemiologic And Phylogenetic Aspects, Eric W. Brown, Naoya Yuhki, Craig Packer, Stephen J. O'Brien

Biology Faculty Articles

Feline immunodeficiency virus (FIV) is a novel lentivirus that is genetically homologous and functionally analogous to the human AIDS viruses, human immunodeficiency virus types 1 and 2. FIV causes immunosuppression in domestic cats by destroying the CD4 T-lymphocyte subsets in infected hosts. A serological survey of over 400 free-ranging African and Asian lions (Panthera leo) for antibodies to FIV revealed endemic lentivirus prevalence with an incidence of seropositivity as high as 90%o. A lion lentivirus (FIV-Ple) was isolated by infection of lion lymphocytes in vitro. Seroconversion was documented in two Serengeti lions, and discordance of mother-cub serological status …

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Enhanced Competitiveness Of Tomato Bushy Stunt Virus Defective Interfering Rnas By Segment Duplication Or Nucleotide Insertion, K. Andrew White, Thomas Jack Morris Sep 1994

Enhanced Competitiveness Of Tomato Bushy Stunt Virus Defective Interfering Rnas By Segment Duplication Or Nucleotide Insertion, K. Andrew White, Thomas Jack Morris

Nebraska Center for Virology: Faculty Publications

We have analyzed atypical tomato bushy stunt virus defective interfering (DI) RNA species which accumulated during a passage series in protoplasts. We present a rationale for the order of appearance of these molecules and show, using competition assays, that either segment duplication or single nucleotide insertion can enhance DI RNA competitiveness. Possible mechanisms for the introduction of the modifications observed in these DI RNAs are discussed.

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A Concentration Technique For Obtaining Viable Cysts Of Blastocystis Hominis From Faeces, V Zaman, K Z. Khan Sep 1994

A Concentration Technique For Obtaining Viable Cysts Of Blastocystis Hominis From Faeces, V Zaman, K Z. Khan

Department of Pathology and Laboratory Medicine

No abstract provided.

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Purification And Characterization Of Two Forms Of A High-Molecular-Weight Cysteine Proteinase (Porphypain) From Porphyromonas Gingivalis, Pawel Ciborowski, Makoto Nishikata, Roger D. Allen, Marilyn S. Lantz Aug 1994

Purification And Characterization Of Two Forms Of A High-Molecular-Weight Cysteine Proteinase (Porphypain) From Porphyromonas Gingivalis, Pawel Ciborowski, Makoto Nishikata, Roger D. Allen, Marilyn S. Lantz

Nebraska Center for Virology: Faculty Publications

Porphyromonas gingivalis, an organism implicated in the etiology and pathogenesis of human periodontal diseases, produces a variety of potent proteolytic enzymes, and it has been suggested that these enzymes play a direct role in the destruction of periodontal tissues. We now report that two cell-associated cysteine proteinases of P. gingivalis W12, with molecular masses of approximately 150 kDa (porphypain-1) and 120 kDa (porphypain-2), as determined by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, have been separated and purified to apparent homogeneity. These proteinases appear to be SDS-stable conformational variants of a 180-kDa enzyme, and they are the largest cysteine proteinases …

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The Production And Characterization Of A Monoclonal Antibody To A Naturally Occurring Aminomalonic Acid Epitope And A Comparison With An Antibody To A Synthetically Derived Aminomalonic Acid Epitope, James L. Gulley Aug 1994

The Production And Characterization Of A Monoclonal Antibody To A Naturally Occurring Aminomalonic Acid Epitope And A Comparison With An Antibody To A Synthetically Derived Aminomalonic Acid Epitope, James L. Gulley

Loma Linda University Electronic Theses, Dissertations & Projects

The goal for this project is to undertake the first step in the study of blocking metastasis by a monoclonal antibody. The long range goal of this line of research is to study a novel mechanism for the blocking of tumor metastasis in general and prostatic cancer metastasis in particular. The problem that this addresses is the growing number of cases of prostatic cancer in the increasingly aging male population. This cancer is not lethal unless it metastasizes, yet there is no cure for the metastatic disease. The inherent specificity of a monoclonal antibody combined with the uniqueness of the …

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Immunohistochemistry In Tumour Diagnosis--Who Actually Needs It?, S Pervez Aug 1994

Immunohistochemistry In Tumour Diagnosis--Who Actually Needs It?, S Pervez

Department of Pathology and Laboratory Medicine

No abstract provided.

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Molecular Studies Of Kpnai And Kpnbi R-M Systems : In Klebsiella Pneumoniae M5a1 And Gm236, Nan Sook Lee Aug 1994

Molecular Studies Of Kpnai And Kpnbi R-M Systems : In Klebsiella Pneumoniae M5a1 And Gm236, Nan Sook Lee

Loma Linda University Electronic Theses, Dissertations & Projects

Two possible type I restriction-modification (R-M) systems, KpnAI and KpnBl, were recognized in Klebsiella pneumoniae strains M5al and GM236, respectively. In this project, four hsdRKpnAI clones (pNLA1 to pNLA4) were obtained by using an endonuclease expression-selection method. The hsdRKpnAI gene was identified on a 3.6 kb Sau3Al fragment. To map the gene, the hsdRKpnAI clone was hybridized to a membrane which contained pulsed-field gel electrophoresed fragments of the chromosome of the strain M5al. The hsdRKpnAI clone was hybridized to a 19 kb Xbal fragment and a 420 kb Blnl fragment, located …

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