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Articles 151 - 180 of 180

Full-Text Articles in Dairy Science

Development Of Endospore-Specific Primers For The Trfp Analysis Of Microbial Population In Milk Powder, M. M. Arendts, Amy J. Rife, Rafael Jiménez-Flores Jan 2002

Development Of Endospore-Specific Primers For The Trfp Analysis Of Microbial Population In Milk Powder, M. M. Arendts, Amy J. Rife, Rafael Jiménez-Flores

Dairy Science

Abstract of paper presented at the 2002 Joint Annual Meeting of the American Dairy Science Association & the American Society of Animal Science.


The Detection Of Bacillus Endospores During Low Heat Skim Milk Powder Processing Using Nucleic Acid Technology, Amy J. Rife, Rafael Jiménez-Flores, Chris Kitts, Mark Kubinski Jan 2002

The Detection Of Bacillus Endospores During Low Heat Skim Milk Powder Processing Using Nucleic Acid Technology, Amy J. Rife, Rafael Jiménez-Flores, Chris Kitts, Mark Kubinski

Dairy Science

Abstract of paper presented at the 2002 Joint Annual Meeting of the American Dairy Science Association & the American Society of Animal Science.


Effects Of Seasonal And Regional Variations In Milk Components On The Buffering Capacity Of Milk In California, A. Harris, Phillip Tong, Sean Vink, Jesus Izco, Rafael Jiménez-Flores Jan 2002

Effects Of Seasonal And Regional Variations In Milk Components On The Buffering Capacity Of Milk In California, A. Harris, Phillip Tong, Sean Vink, Jesus Izco, Rafael Jiménez-Flores

Dairy Science

Abstract of paper presented at the 2002 Joint Annual Meeting of the American Dairy Science Association & the American Society of Animal Science.


Disulfide Bonding Patterns Between Β-Lactoglobulin And Κ-Casein In A Heated And Spray-Dried Milk-Model, Annie Bienvenue, C. S. Norris, Michael J. Boland, Lawrence K. Creamer, Rafael Jiménez-Flores Jan 2002

Disulfide Bonding Patterns Between Β-Lactoglobulin And Κ-Casein In A Heated And Spray-Dried Milk-Model, Annie Bienvenue, C. S. Norris, Michael J. Boland, Lawrence K. Creamer, Rafael Jiménez-Flores

Dairy Science

Abstract of paper presented at the 2002 Joint Annual Meeting of the American Dairy Science Association & the American Society of Animal Science.


Extraction Of Lipids From Buttermilk Using Supercritical Carbon Dioxide, Harit K. Vyas, Johanna C. Astaire, Rafael Jiménez-Flores Jan 2002

Extraction Of Lipids From Buttermilk Using Supercritical Carbon Dioxide, Harit K. Vyas, Johanna C. Astaire, Rafael Jiménez-Flores

Dairy Science

Abstract of paper presented at the 2002 Joint Annual Meeting of the American Dairy Science Association & the American Society of Animal Science.


Monitoring Endospores And Endospore-Forming Bacteria Populations In Commercial Skim Milk Powder Production Plants, C. Murillo, Rafael Jiménez-Flores Jan 2002

Monitoring Endospores And Endospore-Forming Bacteria Populations In Commercial Skim Milk Powder Production Plants, C. Murillo, Rafael Jiménez-Flores

Dairy Science

Abstract of paper presented at the 2002 Joint Annual Meeting of the American Dairy Science Association & the American Society of Animal Science.


Heat-Induced Reactions Involving Β-Lactoglobulin And Other Milk Proteins In Milk, Whey, And Model Systems, Lawrence K. Creamer, G. A. Manderson, Y. H. Hong, P. Havea, Y. H. Cho, H. Singh, Annie Bienvenue, Rafael Jiménez-Flores Jan 2002

Heat-Induced Reactions Involving Β-Lactoglobulin And Other Milk Proteins In Milk, Whey, And Model Systems, Lawrence K. Creamer, G. A. Manderson, Y. H. Hong, P. Havea, Y. H. Cho, H. Singh, Annie Bienvenue, Rafael Jiménez-Flores

Dairy Science

Abstract of paper presented at the 2002 Joint Annual Meeting of the American Dairy Science Association & the American Society of Animal Science.


Sensory And Instrumental Measurements Of The Sensory Properties Of Powdered Buttermilk, M. Spill, Jean-Xavier Guinard, Rafael Jiménez-Flores Jan 2002

Sensory And Instrumental Measurements Of The Sensory Properties Of Powdered Buttermilk, M. Spill, Jean-Xavier Guinard, Rafael Jiménez-Flores

Dairy Science

Abstract of paper presented at the 2002 Joint Annual Meeting of the American Dairy Science Association & the American Society of Animal Science.


Validation Of Capillary Electrophoresis For The Ultra-Rapid Determination Of Inorganic Phosphate And Citrate In Milk, Jesus M. Izco, Monica Tormo, Phillip Tong, Rafael Jiménez-Flores Jan 2002

Validation Of Capillary Electrophoresis For The Ultra-Rapid Determination Of Inorganic Phosphate And Citrate In Milk, Jesus M. Izco, Monica Tormo, Phillip Tong, Rafael Jiménez-Flores

Dairy Science

Abstract of paper presented at the 2002 Joint Annual Meeting of the American Dairy Science Association & the American Society of Animal Science.


Utilization Of Dot Blots To Screen Probiotic Lactobacilli For Mucin Binding, J. Newman, Rafael Jiménez-Flores Jan 2002

Utilization Of Dot Blots To Screen Probiotic Lactobacilli For Mucin Binding, J. Newman, Rafael Jiménez-Flores

Dairy Science

Abstract of paper presented at the 2002 Joint Annual Meeting of the American Dairy Science Association & the American Society of Animal Science.


Scale Up And Mass Balance Of Affinity Purification Of Native Β-Lactoglobulin, Harit K. Vyas, Jesus M. Izco, Rafael Jiménez-Flores Jan 2001

Scale Up And Mass Balance Of Affinity Purification Of Native Β-Lactoglobulin, Harit K. Vyas, Jesus M. Izco, Rafael Jiménez-Flores

Dairy Science

Abstract of paper presented at the 54th Annual Reciprocal Meat Conference (2001).


Use Of Capillary Electrophoresis (Ce) To Determine Metabolic Organic Acids In Milk, Jesus M. Izco, Monica Tormo, Rafael Jiménez-Flores Jan 2001

Use Of Capillary Electrophoresis (Ce) To Determine Metabolic Organic Acids In Milk, Jesus M. Izco, Monica Tormo, Rafael Jiménez-Flores

Dairy Science

Abstract of paper presented at the 54th Annual Reciprocal Meat Conference (2001).


Screening And Binding Characterization Of Lactic Acid Bacteria With Milk Fat Globular Membrane Components, D. T. Elizonado-Bachiero, A. Benavides, Rafael Jiménez-Flores Jan 2001

Screening And Binding Characterization Of Lactic Acid Bacteria With Milk Fat Globular Membrane Components, D. T. Elizonado-Bachiero, A. Benavides, Rafael Jiménez-Flores

Dairy Science

Abstract of paper presented at the 2001 American Society for Microbiology 104th General Meeting: New Orleans, LA.


Evaluation Of Commercial Adjuncts For Use In Cheese Ripening : 4. Comparison Between Attenuated And Not Attenuated Lactobacilli, M. El-Soda, S. A. Madkor, P. S. Tong Jan 2000

Evaluation Of Commercial Adjuncts For Use In Cheese Ripening : 4. Comparison Between Attenuated And Not Attenuated Lactobacilli, M. El-Soda, S. A. Madkor, P. S. Tong

Dairy Science

No abstract provided.


Evaluation Of Commercial Adjuncts For Use In Cheese Ripening: 5. Effect Of Added Freeze-Shocked Adjunct Lactobacilli On Proteolysis And Sensory Quality Of Reduced Fat Cheddar Cheese, S. A. Madkor, P. S. Tong, M. El Soda Jan 2000

Evaluation Of Commercial Adjuncts For Use In Cheese Ripening: 5. Effect Of Added Freeze-Shocked Adjunct Lactobacilli On Proteolysis And Sensory Quality Of Reduced Fat Cheddar Cheese, S. A. Madkor, P. S. Tong, M. El Soda

Dairy Science

No abstract provided.


Modifications Of Phosphocaseinate Solutions Treated By Selective Adsorption, Nathalie Martin, Rafael Jiménez-Flores, Phillip S. Tong, Yves Pouliot, Michel Britten Mar 1999

Modifications Of Phosphocaseinate Solutions Treated By Selective Adsorption, Nathalie Martin, Rafael Jiménez-Flores, Phillip S. Tong, Yves Pouliot, Michel Britten

Dairy Science

The treatment of phosphocaseinate by adsorption with diatomaceous earths has been studied. The results suggest that magnesium and calcium silicates have a specific affinity for milk proteins. As a result, the ratio of individual casein types in phosphocaseinate solution can be manipulated.


Evaluation Of Commercial Adjuncts For Use In Cheese Ripening: 1. Enzymatic Activities And Autolytic Properties Of Freeze-Shocked Adjuncts In Buffer System, M. El-Soda, S. A. Madkor, P. S. Tong Jan 1999

Evaluation Of Commercial Adjuncts For Use In Cheese Ripening: 1. Enzymatic Activities And Autolytic Properties Of Freeze-Shocked Adjuncts In Buffer System, M. El-Soda, S. A. Madkor, P. S. Tong

Dairy Science

No abstract provided.


Evaluation Of Commercial Adjuncts For Use In Cheese Ripening: 3. Properties Of Heat-Shocked Adjuncts In Buffer And Cheese Slurry Systems, M. El-Soda, S. A. Madkor, P. S. Tong Jan 1999

Evaluation Of Commercial Adjuncts For Use In Cheese Ripening: 3. Properties Of Heat-Shocked Adjuncts In Buffer And Cheese Slurry Systems, M. El-Soda, S. A. Madkor, P. S. Tong

Dairy Science

No abstract provided.


Evaluation Of Commercial Adjuncts For Use In Cheese Ripening: 2. Ripening Aspects And Flavor Development In Cheese Curd Slurries Prepared With Adjunct Lactobacilli, S. A. Madkor, M. El-Soda, P. S. Tong Jan 1999

Evaluation Of Commercial Adjuncts For Use In Cheese Ripening: 2. Ripening Aspects And Flavor Development In Cheese Curd Slurries Prepared With Adjunct Lactobacilli, S. A. Madkor, M. El-Soda, P. S. Tong

Dairy Science

No abstract provided.


Food Choices For The 21st Century, Louise Berner, Nancy Childs, Constance Geiger, Carl Keen, Janet King, Janet Mcdonald, Sanford Miller, Suzanne Murphy, Harold Schmitz, Anne Shaw, Phil Tong, Connie Weaver Jan 1999

Food Choices For The 21st Century, Louise Berner, Nancy Childs, Constance Geiger, Carl Keen, Janet King, Janet Mcdonald, Sanford Miller, Suzanne Murphy, Harold Schmitz, Anne Shaw, Phil Tong, Connie Weaver

Dairy Science

In December 1997, a coalition of health professional with a wide range of expertise in science, government, education, marketing and communications, convened to discuss the challenges and implications of food choices for the 21st Century. Taking the newly published Dietary Reference Intakes (DRIs) as a framework and starting point, the members of the coalition explored how health professionals can individualize dietary recommendation to help guide people in their food choices.


Whey Protein Edible Film Structures Determined By Atomic Force Microscope, L.E. Lent, L. S. Vanasupa, P. S. Tong Sep 1998

Whey Protein Edible Film Structures Determined By Atomic Force Microscope, L.E. Lent, L. S. Vanasupa, P. S. Tong

Dairy Science

Atomic force microscopy was used to study edible films produced from whey proteins. The films were imaged under ambient conditions with no special sample preparation. Low resolution imaging of areas from 10 μm to 150 μm on a side was performed in the contact mode. Higher resolution scans of 350 nm to 2,700 nm required use of the noncontact imaging mode. Features about the same size as the primary protein in whey, beta-lactoglobulin (7 nm), were identified in the film samples. Molecular aggregates in the range of 1 μm, reported in other studies using transmission electron microscopy of whey protein …


Expression Of A Bovine Κ-Cn Cdna In The Mammary Gland Of Transgenic Mice Utilizing A Genomic Milk Protein Gene As An Expression Cassette, Alfonso Gutiérrez, Harry M. Meade, Paul Ditullio, Daniel Pollock, Merry Harvey, Rafael Jiménez-Flores, Gary B. Anderson, James D. Murray, Juan F. Medrano Jul 1996

Expression Of A Bovine Κ-Cn Cdna In The Mammary Gland Of Transgenic Mice Utilizing A Genomic Milk Protein Gene As An Expression Cassette, Alfonso Gutiérrez, Harry M. Meade, Paul Ditullio, Daniel Pollock, Merry Harvey, Rafael Jiménez-Flores, Gary B. Anderson, James D. Murray, Juan F. Medrano

Dairy Science

Transgenic mice were produced by microinjection of a DNA construct composed of the bovine κ-casein (κ-CN) cDNA under the control of the goat β-CN 5' promoter elements and 3' flanking regions into pronuclear-stage embryos. The gene construct targeted the expression of bovine κ-CN RNA to the mammary gland and secretion of bovine κ-CN in the milk. In the three lines studied (BC-7, BC-31 and BC-67) the transgene was stably integrated and propagated as a Mendelian locus. Expression of the bovine protein in lactating mice from the three transgenic lines was demonstrated by northern and western blots. In ten different tissues …


Abnormal Properties Of Milk From Transgenic Mice Expressing Bovine Β-Casein Under Control Of The Bovine Α-Lactalbumin 5' Flanking Region, Rafael Jiménez-Flores, Gregory T. Bleck, Robert D. Bremel Jan 1995

Abnormal Properties Of Milk From Transgenic Mice Expressing Bovine Β-Casein Under Control Of The Bovine Α-Lactalbumin 5' Flanking Region, Rafael Jiménez-Flores, Gregory T. Bleck, Robert D. Bremel

Dairy Science

Transgenic mice produced in this investigation contained the proximal promoter elements, the genomic coding sequence and the 3' flanking region of bovine β-casein and the 5' regulatory elements of bovine α-lactalbumin. Specifically, the gene construct included the TATAA box, CAAT box and polyadenylation signal of bovine β-casein in addition to the 5' regulatory elements of the bovine α-lactalbumin gene. Four lines of transgenic mice were generated; they expressed bovine β-casein in their milk at concentrations of up to 10 mg mL-1. The milk from the transgenic mice tended to be very viscous and a proportion of these mice …


Influence Of Genetic Variants Of Κ-Casein And Β-Lactoglobulin In Milk On Proteolysis In Cheddar Cheese, G. I. Imafidon, N. Y. Farkye, Phillip S. Tong, V. R. Harwalkar Jan 1995

Influence Of Genetic Variants Of Κ-Casein And Β-Lactoglobulin In Milk On Proteolysis In Cheddar Cheese, G. I. Imafidon, N. Y. Farkye, Phillip S. Tong, V. R. Harwalkar

Dairy Science

No abstract provided.


Process To Remove Cholesterol From Dairy Products, Thomas Richardson, Rafael Jiménez-Flores Jul 1994

Process To Remove Cholesterol From Dairy Products, Thomas Richardson, Rafael Jiménez-Flores

Dairy Science

The present invention relates to a process for the removal of cholesterol from a processed or unprocessed dairy product, which process comprises: (a) obtaining a processed or unprocessed dairy product containing cholesterol (b) contacting the dairy product at a temperature of between about 35° and 80° C. with an amount of saponin effective to bind up to about 90% of the cholesterol present in the dairy product; (c) contacting a temperature of between about 35° and 80° C the cholesterol: saponin with an effective amount of diatomaceous earth; (d) separating the insoluble cholesterol: saponin/diatomaceous earth by filtration or centrifugation; and …


Two-Dimensional Analysis Of Skim Milk Proteins Using Preparative Isoelectric Focusing Followed By Polyacrylamide Gel Electrophoresis, Hye-Hyun Y. Kim, Rafael Jiménez-Flores Oct 1992

Two-Dimensional Analysis Of Skim Milk Proteins Using Preparative Isoelectric Focusing Followed By Polyacrylamide Gel Electrophoresis, Hye-Hyun Y. Kim, Rafael Jiménez-Flores

Dairy Science

A preparative isoelectric focusing (IEF) method was applied to separate skim milk proteins using the Rotofor device in a pH 3-10 gradient containing 4 M urea/1% triton X-100. Each of the 20 fractions obtained from the Rotofor device was then analyzed by polyacrylamide gel electrophoresis (PAGE). Both urea-PAGE and SDS-PAGE were used to separate purified caseins and skim milk resulting in comparable two-dimensional pattern. The major bovine caseins (αs1, αs2, β, κ-casein) were resolved better on urea-PAGE. The αs1- and β -casein were focused at pH ~ 4.5 and 4.8, respectively, whereas αs2 …


Low-Fat Dairy Coffee Whitener, Frank V. Kosikowski, Rafael Jiménez-Flores Aug 1987

Low-Fat Dairy Coffee Whitener, Frank V. Kosikowski, Rafael Jiménez-Flores

Dairy Science

A coffee whitener composition made from decreamed milk ultrafiltration retentate having its protein concentrated between about 2:1 to about 4:1 compared to the protein content of the starting milk and a minor whitening power enhancing amount is added in the form of riboflavin (a vitamin) and beta carotene (a vitamin pre-cursor) or a mixture thereof. The composition is usually in powdered form and is high in protein, low in fat and sodium and reduced in lactose and its high protein enhanced as described provided a low-fat dairy coffee whitening function approximately equal to non-diary coffee creamers.


Method For Removal Of Pharmaceutical Antibiotics From Contaminated Milks, Frank V. Kosikowski, Rafael Jiménez-Flores Aug 1987

Method For Removal Of Pharmaceutical Antibiotics From Contaminated Milks, Frank V. Kosikowski, Rafael Jiménez-Flores

Dairy Science

A method of removing penicillin G and/or pharmaceutical antibiotics which contaminated milk by:
(a) Subjecting the contaminated milk to an ultrafiltration process which produces a permeate containing the contamining antibiotic and a retentate comprising milk proteins and fats.
(b) Adding a non-antibiotic containing retentate diluting (washing) aqueous fluid including uncontaminated ultrafiltered milk permeate, uncontaminated whole milk, uncontaminated skim milk, or uncontaminated acid or sweet whey, or a mixture thereof to the retentate to dilute the retentate.
(c) Subjecting the diluted (washed) retentate to the ultrafiltration process from additional antibiotic containing permeate thereby forming a milk product comprising retentate having a …


Cloning And Sequence Analysis Of Bovine Β-Casein Cdna, Rafael Jiménez-Flores, Young C. Kang, Tom Richardson Jan 1987

Cloning And Sequence Analysis Of Bovine Β-Casein Cdna, Rafael Jiménez-Flores, Young C. Kang, Tom Richardson

Dairy Science

A bovine β-casein cDNA clone was isolated from a cDNA library prepared from mammary gland mRNA. Sequence analysis revealed 25 nucleotides (nt) of the 5′ noncoding region, 672 nt of the complete sequence coding and a 3′ region of approximately 500 nt. When the nucleotide sequence of bovine β-casein cDNA is compared to rat β-casein cDNA (5), a high degree of homology is observed in the first 100 nt corresponding to the signal peptide of the pre-β-caseins.


Nonfat Dairy Coffee Whitener Made From Ultrafiltered Skimmilk Retentates, Rafael Jiménez-Flores, Frank V. Kosikowski Jan 1986

Nonfat Dairy Coffee Whitener Made From Ultrafiltered Skimmilk Retentates, Rafael Jiménez-Flores, Frank V. Kosikowski

Dairy Science

Retentates of different protein concentrations obtained by ultrafiltration of skimmilk were freeze-dried and evaluated as nonfat dairy coffee whiteners. Blended in hot coffee the retentate whiteners containing added riboflavin gave a pH of 6.3-6.55, coffee whitening capacity comparable to a commercial nondairy coffee creamer, and acceptable dispersibility. The retentate nondairy whitener with optimum qualities contained 56% total protein, 0.5% fat, 31.0% carbohydrates, 1.92% calcium, and 27 mg sodium/100g.