Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Dairy Science
Expression Of A Bovine Κ-Cn Cdna In The Mammary Gland Of Transgenic Mice Utilizing A Genomic Milk Protein Gene As An Expression Cassette, Alfonso Gutiérrez, Harry M. Meade, Paul Ditullio, Daniel Pollock, Merry Harvey, Rafael Jiménez-Flores, Gary B. Anderson, James D. Murray, Juan F. Medrano
Expression Of A Bovine Κ-Cn Cdna In The Mammary Gland Of Transgenic Mice Utilizing A Genomic Milk Protein Gene As An Expression Cassette, Alfonso Gutiérrez, Harry M. Meade, Paul Ditullio, Daniel Pollock, Merry Harvey, Rafael Jiménez-Flores, Gary B. Anderson, James D. Murray, Juan F. Medrano
Dairy Science
Transgenic mice were produced by microinjection of a DNA construct composed of the bovine κ-casein (κ-CN) cDNA under the control of the goat β-CN 5' promoter elements and 3' flanking regions into pronuclear-stage embryos. The gene construct targeted the expression of bovine κ-CN RNA to the mammary gland and secretion of bovine κ-CN in the milk. In the three lines studied (BC-7, BC-31 and BC-67) the transgene was stably integrated and propagated as a Mendelian locus. Expression of the bovine protein in lactating mice from the three transgenic lines was demonstrated by northern and western blots. In ten different tissues …