Biomedical Engineering and Bioengineering Commons^™

Involvement Of Long Non-Coding Rna (Lncrna) Malat1 In Shear Stress Regulated Adipocyte Differentiation, Justin Caron, Marjan Ghanbariabdolmaleki, Madison Marino, Chong Qiu, Bo Wang, Michael Mak, Shue Wang

Biomedical Engineering Faculty Research and Publications

Adipocyte differentiation plays an important role in bone remodeling due to secretory factors that can directly modulate osteoblast and osteoclast, thus affecting overall bone mass and skeletal integrity. Excessive adipocyte differentiation within the bone marrow microenvironment can lead to decreased bone mass, eventually causing osteoporosis. The mechanical microenvironment of bone marrow, including fluid shear, maintains the balance of adipocyte and osteoblast differentiation during bone remodeling. However, how mechanical cues interact with long noncoding RNA (lncRNA) and regulate adipocyte differentiation remains unexplored. In this study, we investigated the mechanosensitive role of lncRNA MALAT1 during mesenchymal stem cells (MSCs) adipocyte differentiation. By …

Computationally Guided Liquid Crystal-Based Competitive Binding Sensing Platform For Optical Detection Of Spike Protein, Homa Ghaiedi, Shubham Pandey, Sophia Ezendu, Tibor Szilvasi, Karthik Nayani

Chemical Engineering Faculty Publications and Presentations

A liquid crystal (LC)-based sensing platform for the rapid optical detection of SARS-CoV-2 ' s spike protein's receptor binding domain (RBD) domain is introduced. This platform utilizes a thermotropic LC, hosted on metal-cation decorated substrates, onto which the spike protein can competitively bind. Density functional theory (DFT) calculations guide the experiments that reveal a homeotropic-to-planar transition in the LCs upon exposure to SARS-CoV-2 spike-decorated yeast, providing a basis for sensitive virus detection. The sensor's reversibility/specificity is confirmed through antibody-induced orientation recovery of the LCs initial orientation. Strikingly, the sensor can detect approximate to 2000 copies of the spike protein per …

Design Of A Teleoperabletendon-Driven Robotic Hand, Zachary Giese, Joseph Gober, Samuel Grisham, Avery Mahan, Aspen White, Brayden May, Aspen White

ATU Scholars Symposium

This work presents the design, implementation, and validation of a 3D-printed tendon-driven robotic hand with a bi-directional wireless control system. The robotic hand emulates human anatomical principles, focusing on replicating flexion and extension mechanisms of the metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints via a tendon-pulley system. Iterative prototyping resolved initial challenges in joint stiffness, tendon routing, and servo interference, culminating in a design where a single servo drives both flexion and extension for each joint using a bidirectional tendon control mechanism. A reliable bidirectional wireless communication framework, using Arduino Nano Every microcontrollers and NRF24L01 modules, enables …

Dust Analyses In Ventilation Ducts, Karolina Vyhlidalova, Petr Vlček, Olga Rubinová, Jiří Bernard

Journal of Sustainable Construction Materials and Technologies

The paper describes the importance of monitoring the dust in the atmosphere of human life. It deals with the issue of dust in air ducts, approaching the methods that can be used to detect the amount of dust. For conditions for buildings in the Czech Republic there is created the methodology of quantitative and qualitative evaluation of dust sediment for the needs of HVAC and other building surfaces. The scale for visual evaluation was created and it has been elaborated the assessment of kind of particles contained in dust.

Assessment Of Long Time Approximation Equation To Determine Thermal Conductivity Of High Porous Materials With Nss Probe, Hussein Humaish, Bastien Ruet, Laurent Marmoret, Hassen Beji

Journal of Sustainable Construction Materials and Technologies

Recent economic changes have increased the focus on energy conservation in buildings. Improve insulation represents one of the biggest challenge to save energy. The TP02 Huksefux® Non Steady State Probe (NSSP) has been used to determine the thermal conductivity of the building insulation materials. Usually, the long term approximation equation (linear form) is applied to determine the thermal conductivity when using hot wire techniques. Long term approximation has been successfully used to characterize glycerol: a fluid which doesn’t present contact resistance and porosity. But, an S-shaped appears for more porous materials. So to generalize the possibility of using this method, …

Analyzing The Chemical Composition & Morphology Of Hydroxyapatite Coating On Magnesium Biomaterials, Khaoula Kamal

Honors Theses

The purpose of this thesis is to further explore the capabilities of magnesium metal to serve in biodegradable orthopedic implant applications. Current standards for orthopedic implants involve metals that cause many issues such as high risk of infection due to the need for secondary surgery. Despite magnesium’s biocompatibility, it presents a challenge that will be addressed in this thesis: a fast degradation rate. To combat this problem, hydroxyapatite (HA), an integral component of bone, was used to coat the surface of magnesium scaffolds and offer a slower degradation rate. Previous Priddy Lab studies have confirmed the presence of HA coating …

Enhanced And Interpretable Prediction Of Multiple Cancer Types Using A Stacking Ensemble Approach With Shap Analysis, Shahid Mohammad Ganie, Pijush Kanti Dutta Pramanik, Zhongming Zhao

Faculty, Staff and Student Publications

Background: Cancer is a leading cause of death worldwide, and its early detection is crucial for improving patient outcomes. This study aimed to develop and evaluate ensemble learning models, specifically stacking, for the accurate prediction of lung, breast, and cervical cancers using lifestyle and clinical data.

Methods: 12 base learners were trained on datasets for lung, breast, and cervical cancer. Stacking ensemble models were then developed using these base learners. The models were evaluated for accuracy, precision, recall, F1-score, AUC-ROC, MCC, and kappa. An explainable AI technique, SHAP, was used to interpret model predictions.

Results: The stacking …

Quantifying Treatment Response To A Macrophage-Targeted Therapy In Combination With Immune Checkpoint Inhibitors After Exposure To Conventional Chemotherapy, Shelby N. Bess, Gaven K. Smart, Timothy J. Muldoon

Biomedical Engineering Faculty Publications and Presentations

Background: Conventional chemotherapeutic agents, such as 5-fluorouracil (5-FU), can exert anti-tumor effects through immunogenic cell death (ICD) induction. Researchers have found hallmarks that quantify ICD (such as the translocation of HMGB1 and calreticulin). Although chemotherapeutic agents can induce ICD, they increase the expression of immune checkpoints, limiting their effectiveness. Studies have emphasized the importance of investigating the heterogeneous responses of cells co-localized in a solid tumor (macrophages, tumor cells, etc.) to ICD induction. However, these studies were performed in vivo, which limits the collection of information on cell-cell interactions due to model complexity.

Methods: In this study, we used a …

Development Of 6d Electromagnetic Actuation For Micro/Nanorobots In High Viscosity Fluids For Drug Delivery, Mostafa Abdelaziz, Maki Habib

Mechanical Engineering

This research focuses on the development, design, implementation, and testing (with complete hardware and software integration) of a 6D Electromagnetic Actuation (EMA) system for the precise control and navigation of micro/nanorobots (MNRs) in high-viscosity fluids, addressing critical challenges in targeted drug delivery within complex biological environments, such as blood vessels. The primary objective is to overcome limitations in the actuation efficiency, trajectory stability, and accurate path-tracking of MNRs. The EMA system utilizes three controllable orthogonal pairs of Helmholtz coils to generate uniform magnetic fields, which magnetize and steer MNRs in 3D for orientation. Another three controllable orthogonal pairs of Helmholtz …

Optimizing Hydrogel Performance Composed Of Japanese Pagoda Tree Extract Loaded-Gelatin-Sodium Alginate-Polyethylene Oxide For Biomedical Applications: Influence Incorporated Calcium-Based Metal Organic Frameworks And Zinc Oxide Nps, Samar A. Salim

Nanotechnology Research Centre

Gelatin/sodium alginate/polyethylene oxide hydrogel was synthesized by self-gelation method and utilized to achieve enhanced mechanical strength. Japanese pagoda, tree extracts rich in flavonoids and isoflavonoids was incorporated to enhance tissue repair along with ZnO-NPs to provide the hydrogel with necessary antimicrobial and other desirable biological properties. The inclusion of Ca-BDC MOF enhanced hydrogel matrix by providing more surface area and the presence of calcium ion needed for tissue regeneration. Results revealed that composite hydrogel displayed suitable gelation time and swelling rate that was further improved by incorporating the plant extract, ZnO-NPs, and Ca-BDC MOF. The efficacy of the tested formulations …

Providing A Solution For Active-Duty Servicewomen Facing Chronic Musculoskeletal Injuries Due To Improper Gear Fit, Celia Watson, Denver Tidwell, Hadi Obaji

Honors Theses

As senior biomedical engineering students at the University of Mississippi, we began our academic year brainstorming unmet clinical needs in healthcare intending to provide an innovative solution. After conducting personal inventories, determining a mission, and evaluating internal and external factors, we established acceptance criteria for our strategic focus and clarified our needs statement: develop a prophylactic device for chronic musculoskeletal injuries (MSKIs) in active-duty servicewomen (ADSW). The average female soldier is at a disadvantage compared to her male counterpart due to anthropometric and physiological differences between men and women in most aspects of physical performance (Epstein et al. 2673). These …

Investigating The Role Of Blood Models In Predicting Rupture Status Of Intracranial Aneurysms, Zonghan Lyu, Mostafa Rezaeitaleshmahalleh, Nan Mu, Jingfeng Jiang

Michigan Tech Publications

Purpose. Selecting patients with high-risk intracranial aneurysms (IAs) is of clinical importance. Recent work in machine learning-based (ML) predictive modeling has demonstrated that lesion-specific hemodynamics within IAs can be combined with other information to provide critical insights for assessing rupture risk. However, how the adoption of blood rheology models (i.e., Newtonian and Non-Newtonian blood models) may influence ML-based predictive modeling of IA rupture risk has not been investigated.Methods and Materials.In this study, we conducted transient CFD simulations using Newtonian and non-Newtonian rheology (Carreau-Yasuda [CY]) models on a large cohort of 'patient-specific' IA geometries (>100) under pulsatile flow conditions to …

Analysis Of Differentially Expressed Genes (Deg) And Upstream Regulator Proteins Indicates That Inhibition Of Transforming Growth Factor Beta 1 (Tgfb1) Is A Potential Target For Acne Inversa, Weri Veranita, Siva Fauziah, Siti Nurbaya

Indonesian Journal of Medical Chemistry and Bioinformatics

Acne inversa (AI) is a chronic inflammatory skin disease characterized by painful nodules, abscesses, and scarring, primarily in intertriginous areas. This study aims to identify potential therapeutic targets for managing acne inversa based on the analysis of differentially expressed genes (DEG). The expression targets of these genes were then validated for their potential as biomarkers, and upstream regulator proteins (URPs) were identified from the resulting DEG. DEG analysis on the GEO dataset GSE122592 (acne inversa vs. healthy donor skin) revealed five DEG that can serve as biomarkers for acne inversa, with a sensitivity and specificity of (100%). These DEG—IL10, GZMB, …

Optimizing A Perfusion-Compression Bioreactor Via Python Integration, Umamah Amer

Honors Theses

Bioreactors are widely used in tissue engineering to support cell and tissue growth under controlled conditions. Perfusion-compression bioreactors, like the one developed in our lab, can be used to replicate the physiological loading of bone on 3D-printed scaffolds. When seeded with osteogenic cells and subjected to mechanical loading, these scaffolds serve as a model to study osteogenesis. To simulate physiological conditions, the bioreactor must apply 1000 compression cycles at forces up to 200 N, three times daily over 14 days. Upon implementation, validation testing and experimental studies exposed several limitations in the bioreactor’s control code. The system was initially programmed …

Evervital Patch: The Design And Development Of A Continuous Cardiac Monitoring Device For Post-Operative, In-Hospital Patients, Abigail Moeller, Jonathan Seid

Honors Theses

In the United States, due to the increasing prevalence of cardiovascular disease, over 900,000 cardiac surgeries are performed annually, with projections exceeding 1.3 million by 2029. Many post-surgical patients require continuous in-hospital cardiac monitoring, which is typically conducted using electrocardiograms (EKGs). However, traditional EKG systems such as bedside cardiac monitors and cardiac telemetry monitors rely on wired leads that can easily tangle, degrade signal quality, and require frequent readjustments or replacements due to wire breakage, and for the patient, this can cause discomfort from prolonged use. To overcome these challenges, our company, CardioSync, aims to develop a wireless cardiac monitoring …

Using Eeg Signal For Smart Health: An Overview, Addison Clark, Chelsea Bonyata, Ishfaq Ahmad, Hansheng Lei, Khalid Saifullah, Victor Ibarra

Informatics and Engineering Systems Faculty Publications

This survey paper aims to provide an overview of the current state of EEG (Electroencephalography) signal technology as it relates to people with disabilities. It will highlight the various methods and techniques employed, discussing their advantages and disadvantages. The paper will also examine the applications of EEG technology in assisting individuals with disabilities, specifically focusing on Brain-Computer Interfaces (BCIs) and assistive device control. By understanding the current state of EEG signal technology, we can identify the opportunities and challenges involved in utilizing this technology to improve the lives of people with disabilities.

Imine Crosslinked, Injectable, And Self-Healing Fucoidan Hydrogel With Immunomodulatory Properties, Asma Talib Qureshi, Shajia Afrin, Saad Asim, Muhammad Rizwan

Michigan Tech Publications

Biomaterials with inherent anti-inflammatory properties and the ability to foster a pro-regenerative environment hold significant promise for enhancing cell transplantation and tissue regeneration. Fucoidan, a sulfated polysaccharide with well-documented immune-regulatory and antioxidant capabilities, offers strong potential for creating such biomaterials. Yet, there is a lack of engineered fucoidan hydrogels that are injectable and provide tunable physicochemical properties. In this study, the ability of fucoidan to undergo periodate-mediated oxidation is leveraged to introduce aldehydes into backbone (oxidized fucoidan, OFu), enabling the formation of reversible, imine-crosslinks with amine-containing molecules such as gelatin. The imine-crosslinked OFu-gelatin hydrogel provided excellent control over gelation rate …

Modular Molecular Design Of Polymerized Pro-Estrogen Materials Enables Controlled Astrocyte Response, Devan L. Puhl, Alexis Ziemba, Samuel A.T. Ellman, Alex Hsu, Jayant Saksena, Penelope Phillips Falcone, Bailey Balouch, Deniz Rende, Tanner Fink, R. Helen Zha, Ryan J. Gilbert, Edmund F. Palermo

Chemical and Biochemical Engineering Faculty Research & Creative Works

The hormone 17β-estradiol (estrogen or "E2") has demonstrated robust neuroprotective properties in countering oxidative stress-induced neurotoxicity, as well as strong neurotrophic properties to promote axonal growth, following injury to the central nervous system (CNS). However, oral or injected E2 is a suboptimal drug, as systemic administration fails to achieve a therapeutic dose at the injury site, in addition to being contraindicated in most male patients. Polymerized pro-drug biomaterials can mitigate these issues by locally releasing small quantities of drug over vastly extended timescales. We sought to study the effect of the biomaterial properties of poly(pro-E2) on astrocyte-surface interactions because astrocytes …

Sustained Release Of Antifibrotic Nintedanib From Polymer Microparticles Reduces Dosing Frequency While Reducing Inflammation In Murine Idiopathic Pulmonary Fibrosis, Emmanuel Einyat Opolot, Filip Goshevski, Rahul Chaudhary, Horst A. Von Recum, Amar B. Desai

Faculty Scholarship

Purpose: Idiopathic pulmonary fibrosis (IPF) is a life-threatening, progressive lung disease with limited therapeutic options, often resulting in poor patient outcomes. Current treatments, such as Nintedanib (NTB) and Pirfenidone (PFD), require frequent administration, leading to adverse effects and low patient adherence. The purpose of this study was to investigate a sustained-release drug delivery system utilizing microparticles (MPs) composed of insoluble beta-cyclodextrin (β-CD) polymers to enhance the bioavailability and extend the release of NTB and PFD. Methods: A multidisciplinary approach, including in silico modeling, in vitro assays, and in vivo studies, was employed to assess the efficacy of β-CD-polymer MPs as …

Evaluating The Effects Of Oxidative Stress On Primary Retinal Pigment Epithelial Cells, Sophia Hessami

Student Research Symposium

Introduction

Age-Related Macular Degeneration (AMD) is the leading cause of vision loss worldwide, affecting 200 million adults over the age of 40

• Affects subretinal layers: retinal pigment epithelium (RPE), Bruch’s membrane, and choroid (Fig. 1)

Problem: Early mechanisms of AMD are not well understood; oxidative stress is often cited as a key contributing factor to the development of AMD.3 Current studies neglect to use primary RPE cells when investigating oxidative stress.

Oxidative Stress occurs when there is an imbalance between reactive oxygen species (ROS) production and cellular antioxidant defenses

• Overabundance of ROS results in damage to tissue and …