Biomedical Engineering and Bioengineering Commons^™

Isolating Human Monocytes And Characterizing Human Macrophage Polarization Through Flow Cytometry And Morphological Analysis, Arthur Peter Klujian

Biomedical Engineering

Peripheral Artery Occlusive Disease (PAOD) is a cardiovascular disease that affects more than 200 million people. It is characterized by atherosclerosis, a biologic process where peripheral arteries are restricted due to a buildup in cholesterol and fats. This eventually leads to ischemia, developing complications such as chronic inflammation and possible limb amputation. The current standard of care for patients includes surgical revascularization and exercise therapy. These treatment strategies have had limited success with alleviating symptoms and restoring perfusion, however, many patients still experience ischemia and do not fully restore blood flow. Further exploration in pursuit of treating these shortcomings led …

Motor Imagery Eeg Decoding For Brain-Computer Interfaces: Structured Representation, Transfer, And Drift, Yiming Shen

Graduate Doctoral Dissertations

Motor imagery EEG decoding is often summarized by the accuracy of a final classifier, but the classifier is only the last stage of the pipeline. Before classification, the signal has already been shaped by preprocessing, feature extraction, source-session organization, and adaptation. This dissertation studies how feature representation, source-session transfer, and drift shape reliable MI-EEG decoding for brain-computer interfaces.

It first studies within-session decoding on public MI-EEG datasets using nested validation that keeps preprocessing, feature fitting, and model selection inside the training folds. This analysis separates gains from feature representation from gains due to nonlinear classification, and relates both comparisons to …

Early Detection Of Alzheimer’S Disease Using Federated Learning Based On Personalized Gans: A Data-Driven Approach, Iffat Fatima, Uzair Iqbal, Uzair Ishtiaq, Muhammad Burhan Ul Islam Khan

Makara Journal of Technology

Alzheimer’s disease (AD) is a complicated neurodegenerative disease marked by the slow worsening of memory and cognitive functions. Early detection is a key to effective disease management, but the creation of reliable machine learning models is not an easy task because medical data are limited, and there are strict privacy requirements, such as GDPR and HIPAA, that inhibit the creation of central databases. In this paper, a new privacy-saving model that combines personalized federated learning (PFL) with generative adversarial networks (GANs) is proposed to diagnose Alzheimer’s disease. Under the proposed method, the synthesis of data using GAN is conducted on …

Probe Cytotoxic And Oxidative Stress Effects Of Nanoplastics On Caco-2 Cells: Insights From Raman Spectroscopy And Machine Learning, Bryan Gustafson, Negar Kosari, Emily Brothersen, Morgan Mosher, Anhong Zhou

Biological Engineering Student Research

Nanoplastics and microplastics have become an increasing ecological and health concern due to their widespread presence in the environment and food chain. In this study, Caco-2 cells were used as an in vitro model of the human intestinal epithelium to investigate the cytotoxic effects of polystyrene nanoparticles and microparticles of varying sizes, concentrations, and surface modification. Oxidative stress and apoptosis were evaluated following particle exposure. Raman spectroscopy, combined with machine learning analysis, was employed to detect and characterize biochemical alterations in the cells. Several peak ratios were selected to cluster data depending on size. A correlation between apoptosis and both …

Compact 1 Ghz Electron Paramagnetic Resonance Spectrometers For Preclinical Imaging Studies, Tanden A. Hovey

Electronic Theses and Dissertations

Magnetic resonance is one of the most generally useful scientific techniques. New horizons of understanding are revealed as technological advances make possible explorations of nuclear spins and electron spins at higher and lower magnetic fields and electromagnetic frequencies. While the greatest current effort is on ever higher magnetic fields and microwave frequencies, both in nuclear magnetic resonance (NMR) and electron paramagnetic resonance (EPR), the Eaton lab focusses on developing high signal-to-noise performance EPR at low microwave frequencies. EPR provides information about O2, pH, viscosity, glutathione concentrations, and reactive oxygen species in living organisms that MRI cannot. To yield clinically or …

A Longitudinal Study Of Achilles Tendon Adaptation In Ncaa Division I Female Gymnasts: Associations With Menstrual Status, Birth Control Use, Pain, Limb Dominance, And Training Exposure, Mattie Jane Hyde

Electronic Theses and Dissertations

This longitudinal study evaluated Achilles tendon (AT) thickness in 22 NCAA Division I (DI) female gymnasts across three consecutive seasons in relation to menstruation, birth control use, pain, limb dominance, training week, and year of collegiate competition. Weekly ultrasound and athlete survey data were analyzed using mixed-effects models for absolute AT thickness and change in thickness from baseline measurements. Associations were primarily characterized by interactions rather than uniform effects of individual predictors. A Pain × Limb Dominance × Week × Year interaction was associated with both thickness outcomes across season-specific and combined analyses, with relationship directions varying by limb, season …

An Investigation Of Pelvic Kinematics And Implications For Total Hip Arthroplasty, Kathryn Holland Colone

Electronic Theses and Dissertations

Total hip arthroplasty (THA) is one of the most commonly performed orthopaedic procedures, with approximately 500,000 surgeries performed annually in the United States alone. Although THA is widely regarded as highly successful, postoperative complications persist, highlighting the multifactorial nature of THA outcomes. Previous studies have demonstrated the importance of implant positioning and soft tissue mechanics, yet challenges remain in optimizing surgical decisions on a patient-specific basis. Recent advances in surgical planning tools have shifted the focus of preoperative planning toward optimizing patient-specific outcomes, highlighting the need to better understand how patient anatomy, surgical technique, and joint mechanics interact to influence …

Plasma-Generated Ozone For Odor Removal, Jordan Marot

Seton Hall University Dissertations and Theses (ETDs)

Ozone (O₃) is gaining popularity for its use in industrial and environmental applications, including water treatment, air purification, disinfection, and odor remediation. As a powerful oxidizing agent, ozone is highly effective in destroying harmful bacteria and degrading hazardous gas pollutants such as hydrogen sulfide (H₂S). Dielectric barrier discharge (DBD) reactors are widely used for commercial ozone production under atmospheric-pressure conditions. However, ozone yield and selectivity are strongly influenced by operating parameters, particularly gas flow rate, which affects residence time, energy transfer, and competing byproduct chemistry.

This study explores the influence of the air flow rate on …

Enhancing Algae Biofilm Quantity And Value Through Controlled Light Intensity For Treatment Of Municipal Anaerobic Digestor Effluent, Joshua D. Wintch, Ronald C. Sims

Biological Engineering Student Research

Rotating algae biofilm reactors (RABRs) have been shown to remove nutrients from wastewater and to cultivate algae for bioproducts. Another key variable in RABR technology for cultivating microalgae biomass is light intensity. The goal of this study was to address a gap in the design and operation of algae biofilm reactors by analyzing the effects of light intensity on biomass quality and quantity, chemical precipitation of phosphorus, and phosphorus uptake into biomass. This study utilized a triplicate laboratory-scale experiment with PAR intensities from 10 to 2000 µmol m⁻²·s⁻¹. Municipal wastewater anaerobic digester effluent (ADE) was utilized …

Deciphering The Rules Of Odor-Driven Learning In The Fruit Fly Olfactory System, Cole B. Hanan

McKelvey School of Engineering Graduate Student Theses & Dissertations

Odorant identity is encoded by patterns of activity across olfactory receptor neurons (ORNs) and their corresponding glomeruli in the antennal lobe (AL). An odorant's valence can be modified by experience, allowing animals to associate previously neutral odors with appetitive or aversive outcomes. However, some odors appear resistant to such reassignment, and the factors determining whether an odor can acquire learned valence and whether that association generalizes to chemically related odors remain poorly understood. This thesis uses Drosophila melanogaster to address this question using a fully automated proboscis extension reflex (PER) conditioning assay. Odorants are presented via a computer-controlled olfactometer, reward …

A Functional Ultrasound Imaging (Fusi) Pipeline For Investigating Brain-Wide Hemodynamics During Torpor-Like Hypothermia, Owen J. Culver

McKelvey School of Engineering Graduate Student Theses & Dissertations

Torpor is a regulated state of reduced metabolic demand that enables animals to conserve energy under unfavorable environmental conditions. The controlled induction of a similar state, termed synthetic torpor, has potential applications in ischemic protection, organ preservation, cancer treatment, radiation protection, and long-term spaceflight. Although neuronal populations within the preoptic hypothalamus have been shown to initiate hypothermic and hypometabolic states, the distributed cerebral hemodynamic dynamics accompanying their induction and maintenance remain poorly understood. In this thesis, we developed an accessible functional ultrasound imaging (fUSI) acquisition and analysis pipeline and applied it to investigate cerebral hemodynamics during chemogenetic activation of the …

Designing And Evolving The Life Support System For Spacex Starship: Toward Closed-Loop Sustainability For Mars Missions, Frányerson R. López Ochoa

Discovery Day - Daytona Beach

The development of a robust Life Support System (LSS) is essential for enabling long-duration human spaceflight beyond Low Earth Orbit (LEO), particularly for missions to Mars. SpaceX Starship represents a next-generation spacecraft designed to support such missions; however, its Environmental Control and Life Support System (ECLSS) remains under active development. This work evaluates the current status of Starship’s LSS by analyzing publicly available data, NASA Human Landing System requirements, and heritage systems such as the International Space Station (ISS) ECLSS and Crew Dragon.

Environmental Ethics Of Plastics And Microplastics Manufacturing: Balancing Industrial Production And Ecological Responsibility, Arindam Gupta

Discovery Day - Daytona Beach

The global production of plastics has increased dramatically over the past several decades, providing substantial economic and societal benefits while simultaneously generating complex environmental and ethical challenges. A growing body of research has identified microplastics—plastic particles smaller than 5 millimeters—as persistent pollutants that accumulate in aquatic ecosystems, soil systems, and the atmosphere. These particles originate both from the degradation of larger plastic products and from direct industrial production processes used in plastics manufacturing. The ethical implications of plastics and microplastics production raise important questions about environmental responsibility, regulatory oversight, and the balance between economic development and ecological sustainability. This project …

A Myoelectric Prosthesis Utilizing A Convolutional Neural Network And Signal Processing, Hope Lea, Katherine Clark, Francis Genco, Carolyn Ascha Richardson, Tobiah Rosser

Discovery Day - Daytona Beach

A Myoelectric Prosthesis Utilizing A Convolutional Neural Network and Signal Processing   Commercially available myoelectric prostheses for transradial amputees utilize electromyography (EMG) sensors to observe electrical signals from superficial muscle contractions and control robotic fingers, yet often have flaws regarding adaptability and dexterity. These devices require users to learn awkward muscle patterns, as the EMG placement and gesture recognition does not consider how humans would normally think about moving their hand. Additionally, the number of available gestures is more limited, as the devices are threshold-based and do not sample enough muscles. This work seeks to address these issues by creating a …

Mri-Based Estimation Of The Hip Joint Center In Infants Using Two Sphere-Fitting Methods, Victoria Melendez

Discovery Day - Daytona Beach

Accurate estimation of the hip joint center (HJC) is critical for biomechanical modeling and clinical assessment. Common methods include predictive methods (e.g., regression equations) and functional methods such as sphere-fitting. In infants, femoral head-based methods are often limited due to incomplete ossification of the anatomy and poor visualization in computed tomography (CT) images. This study evaluates an alternative approach using MRI-based sphere fitting of the ossified acetabular rim, comparing its accuracy to traditional femoral head-based methods. MRI scans of three male infants (ages 10.7- 12.7 months) were used to manually place landmarks on the ossified acetabulum and the cartilaginous boundary …

Apical-Out Lung Organoids: A Platform For Spaceflight And Cf Research, Valeria Jimenez, Ally Schwennesen

Discovery Day - Daytona Beach

A genetic condition known as cystic fibrosis (CF) is brought on by mutations in the CFTR gene, which results in chronic lung inflammation and impaired chloride ion transport. CF bronchial epithelial cells enable direct comparison of disease pathology, while normal human bronchial epithelial (NHBE) cells offer an essential model for researching airway physiology. Realistic modeling of respiratory tissue and its reaction to environmental stressors like microgravity is made possible by the creation of 3D lung organoids from these cells. In this project, we developed apical-out organoids, which expose the apical surface of epithelial cells to the external environment. These organoids …

Assessing Non-Thermal Plasma In Cutaneous Regeneration, Arden Rodriguez, Meliah Martin, Graeme Grainger, Arineh Shahbazi

Discovery Day - Daytona Beach

Chronic and acute wounds remain a significant clinical challenge, particularly in austere and resource-limited environments where access to advanced care is restricted. Non-Thermal Plasma (NTP) has emerged as a promising, non-invasive modality with reported antimicrobial and regenerative properties; however, its mechanisms of action and effects on human skin tissue require further characterization. The scope of this project is to engineer and utilize a 3D human skin epithelial model to evaluate the effects of NTP on wound healing. We will first build a 3D epithelial tissue model that will simulate human skin, followed by modeling and evaluation of wound pathology and …

Restoration And Instrumentation Of A Cantilever Beam Model Using Strain Gauge Measurement, Ana Paula Guevara

Discovery Day - Daytona Beach

This project aims to restore and modernize an instructional cantilever beam model with a C‑shaped cross‑section used in Aerospace Structures courses, addressing the need for updated, hands‑on teaching tools that connect theoretical stress–strain concepts to real experimental data. The existing model, previously limited to qualitative demonstrations, is being rehabilitated and instrumented with a bonded strain gauge capable of detecting resistance changes under applied bending loads. These signals are processed through an Arduino‑based data acquisition system, which converts electrical resistance into strain values and displays them in real time on an LCD interface. The project’s objectives are to return the beam …

Interactive Effects Of Tissue Temperature And Hydration State On Tendon Mechanical Properties Using A Chicken Drumstick Model, Makayla Maso, Kiara Maso, Mikayla Hulvey

Discovery Day - Daytona Beach

This study focused on the effects of temperature and hydration on tendon extensibility. Four experimental groups were tested using chicken leg tendons tested ex vivo: cold and dehydrated, cold and hydrated, warm and dehydrated, and warm and hydrated. Each treatment group was pulled with an increasing load and measured. It was hypothesized that the warm and hydrated test group would extend the furthest. This was based on studies citing increased bodily flexibility with increasing body temperature as well as stiffness at lower temperatures. Combining temperature and hydration state allowed for not only individual effects of variables to be tested but …

Simulating Pacemakers And Heartbeat Recovery Through Mathematical Modeling, Thomas Estrada, Jayla Edwards

Discovery Day - Daytona Beach

Title: Simulating Pacemakers and Heartbeat Recovery Through Mathematical Modeling   This study utilizes the Fitzhugh-Nagumo model to simulate cardiac electrical activity and the regulatory role of pacemakers through ordinary differential equations (ODEs). By defining the rate of change for membrane voltage, 𝑑𝑣/dt, and a recovery variable, 𝑑𝑤/dt, the model captures the heart's excitability and resting states. Central to the analysis is the stimulus current parameter, which represents the "kick" provided by a pacemaker to correct flatline conditions or weak heartbeats. Using Euler’s method for numerical integration, the research compares unstable cardiac rhythms against corrected periodic oscillations. Additionally, the project implements vector …