Immunofluorescence Image Feature Analysis And Clustering Pipeline For Distinguishing Epithelial-Mesenchymal Transition, 2019 Virginia Commonwealth University
Immunofluorescence Image Feature Analysis And Clustering Pipeline For Distinguishing Epithelial-Mesenchymal Transition, Shreyas Hirway, Nadiah Hassan, Dr. Christopher Lemmon, Dr. Seth Weinberg
Biology and Medicine Through Mathematics Conference
No abstract provided.
Quantified Measurement Of The Tilt Effect In A Family Of Café Wall Illusions, 2019 Flinders University
Quantified Measurement Of The Tilt Effect In A Family Of Café Wall Illusions, Nasim Nematzadeh Dr., David Martin Powers Prof.
MODVIS Workshop
This abstract explores the tilt effect in a family of Café Wall illusions using a Classical Gaussian Receptive Field model (CRF). Our model constructs an intermediate representation called edge map at multiple scales (Fig. 1) that reveals tilt cues and clues involved in the illusory perception of the Café Wall pattern. We investigate a wide range of parameters of the stimulus including mortar width, luminance, tiles contrast, and phase of the tile displacement (the stimuli in Fig. 2). We show that this simple bioplausible model, simulating the contrast sensitivity of the retinal ganglion cells, can not only detect the tilts …
The 3d Scaffold Design Of De Novo Anterior Cruciate Ligament Graft, 2019 Louisiana State University
The 3d Scaffold Design Of De Novo Anterior Cruciate Ligament Graft, Dominique Angibeau
Honors Theses
No abstract provided.
Stability Analysis Of A More General Class Of Systems With Delay-Dependent Coefficients, 2019 Laboratoire des Signaux et Syst`emes (L2S) CentraleSup´elec-CNRS-Universit´e Paris Sud, 3 rue Joliot- Curie 91192 Gif-sur-Yvette cedex, France.
Stability Analysis Of A More General Class Of Systems With Delay-Dependent Coefficients, Chi Jin, Keqin Gu, Islam Boussaada, Silviu-Iulian Niculescu
SIUE Faculty Research, Scholarship, and Creative Activity
This paper presents a systematic method to analyse the stability of systems with single delay in which the coefficient polynomials of the characteristic equation depend on the delay. Such systems often arise in, for example, life science and engineering systems. A method to analyze such systems was presented by Beretta and Kuang in a 2002 paper, but with some very restrictive assumptions. This work extends their results to the general case with the exception of some degenerate cases. It is found that a much richer behavior is possible when the restrictive assumptions are removed. The interval of interest for the …
Volumetric Muscle Loss: The Role Of Physical Activity And Autologous Repair On Force Recovery And Signaling Pathways, 2019 University of Arkansas, Fayetteville
Volumetric Muscle Loss: The Role Of Physical Activity And Autologous Repair On Force Recovery And Signaling Pathways, Richard Perry
Graduate Theses and Dissertations
Volumetric muscle loss affects both military and civilian persons. The hallmark of this injury is incomplete muscle regeneration, excessive fibrosis, and chronic inflammatory signaling resulting in permanent functional loss. Since permanent functional loss drastically reduces quality of life, many studies have been conducted to improve force recovery. Current scientific literature considers a repair strategy of either devitalized scaffolds infused with growth factors or viable tissue plus activating factors to be the more promising interventions for optimal force recovery. PURPOSE The purpose of this study is to incorporate autologous repair and physical activity and observe the effects of muscle force recovery …
Predictive Modeling Of Fate And Transport Of Three Prevalent Contaminants In Midwest Agroecosystem Surface Waters: Nitrate-N, Atrazine, And Escherichia Coli, 2019 University of Nebraska - Lincoln
Predictive Modeling Of Fate And Transport Of Three Prevalent Contaminants In Midwest Agroecosystem Surface Waters: Nitrate-N, Atrazine, And Escherichia Coli, Samuel Hansen
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
The majority of streams and rivers in the United States (U.S.) are ecologically impaired, or threatened by anthropogenic stressors. Recent reports have found atrazine in drinking water to be associated with increased birth defects and incidences of Non-Hodgkin’s Lymphoma, with higher levels of significance from exposure to both atrazine and nitrate-N. In contrast, recent illnesses from E. coli contaminating vegetables that originated from irrigation water has increased awareness of identifying sources of E. coli entering irrigation reservoirs.
Methods to accurately predict atrazine and E. coli occurrence and potential sources in waterways continue to limit the identifying appropriate and effective prevention …
Hydrodeoxygenation Of Pinyon-Juniper Catalytic Pyrolysis Oil, 2019 Utah State University
Hydrodeoxygenation Of Pinyon-Juniper Catalytic Pyrolysis Oil, Hossein Jahromi
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Catalytic hydrodeoxygenation (HDO), is an effective process to convert oxygenated compounds to hydrocarbons. This process is widely used for improving the negative properties of biomass-derived pyrolysis oils (bio-oils) such as high acidity, poor stability, and low heating value. During this process oxygen is removed from the bio-oil in the form of water, thus the liquid product of HDO process consists of aqueous phase and hydrocarbon phase that can be easily separated. Synthesis of efficient HDO catalyst has been a major challenge in the field of bio-oil upgrading. Red mud, which is an alkaline waste from alumina industry was used to …
Assessing Commonly Used Methods In Measuring Yield Of Cellulose Nanocrystals, 2019 University of Arkansas, Fayetteville
Assessing Commonly Used Methods In Measuring Yield Of Cellulose Nanocrystals, Marilyn Pharr
Biological and Agricultural Engineering Undergraduate Honors Theses
Cellulose is a ubiquitous, renewable biopolymer found in plants that can be broken down to isolate cellulose nanocrystals (CNCs). CNCs have been utilized in various applications that include biomedical technology, structural composites, and barrier films because of their unique mechanical, optical, and physicochemical properties. CNCs can be produced by a variety of approaches from cellulosic materials; however, strong acid hydrolysis is the most common and effective technique as it results in stable colloidal suspensions. Existing literature reveals a wide range of CNC yields, depending on the production process, raw material used, and the method of yield estimation. The yields of …
Final Design Report For The Bioburden Pre-Cleaning Device And Dr. Mark Rasnake At The University Of Tennessee Medical Center, 2019 University of Tennessee
Final Design Report For The Bioburden Pre-Cleaning Device And Dr. Mark Rasnake At The University Of Tennessee Medical Center, Katherine Elizabeth Stiles, Megan Pitz, Kayla Franklin, Simran Dayal, Austin Bullock
Chancellor’s Honors Program Projects
No abstract provided.
Ecological Co2 Flux Of A Green Roof Ecosystem And A Typical Grassland Ecosystem, 2019 University of Arkansas, Fayetteville
Ecological Co2 Flux Of A Green Roof Ecosystem And A Typical Grassland Ecosystem, Madeline Oxner
Biological and Agricultural Engineering Undergraduate Honors Theses
The Hillside Auditorium Green Roof is a low impact development feature on the University of Arkansas campus. It retains storm water and allows plants living on the roof to take up and transpire the water. Green roofs work to mimic natural ecosystems in urban environments. A key property is ecosystem respiration, which plays a large role in the global carbon cycle and is an important biologic activity indicator. The ecosystem respiration of Hillside Auditorium Green Roof was compared to a typical grassland ecosystem at the University of Arkansas farm to determine how closely the green roof is able to mimic …
In Vitro Simulation Of Microgravity Induced Muscle Loss Successfully Increases Expression Of Key In Vivo Atrophy Markers, 2019 Utah State University
In Vitro Simulation Of Microgravity Induced Muscle Loss Successfully Increases Expression Of Key In Vivo Atrophy Markers, Charles P. Harding
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Muscle loss from lack of activity is a serious issue for immobilized patients on Earth and in human spaceflight, where the low gravity environment prevents normal muscle activity. Simulating muscle loss in cultured cells is an important step in understanding how this condition occurs. This work evaluates different means of simulating muscle loss and selects the one that most closely mimics the cellular responses seen in animals and humans.
To simulate the microgravity environment of spaceflight, mouse skeletal muscle cells were grown in a rotary cell culture system (RCCS). Growing the cells within a natural gelled substrate was compared against …
Incorporation Of Egfr And Ron Receptors Into Nanodiscs, 2019 University of New Mexico
Incorporation Of Egfr And Ron Receptors Into Nanodiscs, Cristina Flores-Cadengo
Biomedical Engineering ETDs
Understanding the structure-function relationship of membrane receptors is essential to comprehend the crosstalk between key signaling pathways. Aberrant trans-activation between receptors can lead to tumorigenesis. Two of these receptors known to be involved in cancer development are receptor tyrosine kinases (RTKs), RON (Recepteur d'Origine Nantais) and EGFR (Epidermal Growth Factor Receptor). There has been evidence of heterodimerization and crosstalk between these two receptors based on co-immunoprecipitation, however the structural requirements behind these interactions remain unknown. Structural studies could provide insights into these RTKs’ modes of dimerization and structure-function relationship. However, structural studies of full-length membrane proteins are often difficult due …
Improved Production Of Antifungal Angucycline Sch47554 By Manipulating Three Regulatory Genes In Streptomyces Sp. Scc-2136, 2019 Utah State University
Improved Production Of Antifungal Angucycline Sch47554 By Manipulating Three Regulatory Genes In Streptomyces Sp. Scc-2136, Ozkan Fidan, Riming Yan, Du Zhu, Jixun Zhan
Biological Engineering Faculty Publications
Sch47554 and Sch47555 are two angucyclines with antifungal activities against various yeasts and dermatophytes from Streptomyces sp. SCC‐2136. The schgene cluster contains several putative regulatory genes. Both schA4 and schA21were predicted as the TetR family transcriptional regulators, whereas schA16shared significant similarity to the AraC family transcriptional regulators. Although Sch47554 is the major product of Streptomyces sp. SCC‐2136, its titer is only 6.72 mg/L. This work aimed to increase the production of this promising antifungal compound by investigating and manipulating the regulatory genes in the Sch47554 biosynthetic pathway. Disruption of schA4and schA16 led to a significant increase …
Use Of Surface-Enhanced Raman Scattering (Sers) Probes To Detect Fatty Acid Receptor Activity In A Microfluidic Device, 2019 Utah State University
Use Of Surface-Enhanced Raman Scattering (Sers) Probes To Detect Fatty Acid Receptor Activity In A Microfluidic Device, Han Zhang, Wei Zhang, Lifu Xiao, Yan Liu, Timothy A. Gilbertson, Anhong Zhou
Biological Engineering Faculty Publications
In this study, 4-mercaptobenzoic acid (MBA)-Au nanorods conjugated with a GPR120 antibody were developed as a highly sensitive surface-enhanced Raman spectroscopy (SERS) probe, and were applied to detect the interaction of fatty acids (FA) and their cognate receptor, GPR120, on the surface of human embryonic kidney cells (HEK293-GPRR120) cultured in a polydimethylsiloxane (PDMS) microfluidic device. Importantly, the two dominant characteristic SERS peaks of the Raman reporter molecule MBA, 1078 cm−1 and 1581 cm−1, do not overlap with the main Raman peaks from the PDMS substrate when the appropriate spectral scanning range is selected, which effectively avoided the …
3d-Printed Culture Plate Insert With Agarose Barrier For The Evaluation Of Stem-Cancer Cell Signaling., 2019 Louisiana State University
3d-Printed Culture Plate Insert With Agarose Barrier For The Evaluation Of Stem-Cancer Cell Signaling., Erika M. Lewis
Honors Theses
No abstract provided.
Material Analysis Of A Tympanostomy Myringotomy Simulator, 2019 Louisiana State University
Material Analysis Of A Tympanostomy Myringotomy Simulator, Abishek Stanley
Honors Theses
No abstract provided.
Implementation Of A Particle Image Velocimetry System For Evaluation Of Microfluidic Chip Flows, 2019 Louisiana State University
Implementation Of A Particle Image Velocimetry System For Evaluation Of Microfluidic Chip Flows, Sheila Mallenahalli
Honors Theses
No abstract provided.
Deswelling Induced Morphological Changes In Dual Ph And Temperature Responsive Ultra-Low Crosslinked Poly (N-Isopropyl Acrylamide)-Co-Acrylic Acid Microgels, 2019 Chapman University
Deswelling Induced Morphological Changes In Dual Ph And Temperature Responsive Ultra-Low Crosslinked Poly (N-Isopropyl Acrylamide)-Co-Acrylic Acid Microgels, Molla R. Islam, Maddie Tumbarello, L. Andrew Lyon
Engineering Faculty Articles and Research
Poly(N-isopropylacrylamide) microgels prepared without exogenous cross-linker are extremely “soft” as a result of their very low cross-linking density, with network connectivity arising only from the self-crosslinking of pNIPAm chains. As a result of this extreme softness, our group and others have taken interest in using these materials in a variety of bioengineering applications, while also pursuing studies of their fundamental properties. Here, we report deswelling triggered structural changes in poly(N-isopropylacrylamide-co-acrylic acid) (ULC10AAc) microgels prepared by precipitation polymerization. Dynamic light scattering suggests that the deswelling of these particles not only depends on the collapse of …
Develop A 3d Neurological Disease Model Of Human Cortical Glutamatergic Neurons Using Micropillar-Based Scaffolds, 2019 Utah State University
Develop A 3d Neurological Disease Model Of Human Cortical Glutamatergic Neurons Using Micropillar-Based Scaffolds, Cheng Chen, Xin Dong, Kai-Heng Fang, Fang Yuan, Yao Hu, Min Xu, Yu Huang, Xixiang Zhang, Danjun Fang, Yan Liu
Biological Engineering Faculty Publications
Establishing an effective three-dimensional (3D) in vitro culture system to better model human neurological diseases is desirable, since the human brain is a 3D structure. Here, we demonstrated the development of a polydimethylsiloxane (PDMS) pillar-based 3D scaffold that mimicked the 3D microenvironment of the brain. We utilized this scaffold for the growth of human cortical glutamatergic neurons that were differentiated from human pluripotent stem cells. In comparison with the 2D culture, we demonstrated that the developed 3D culture promoted the maturation of human cortical glutamatergic neurons by showing significantly more MAP2 and less Ki67 expression. Based on this 3D culture …
Building The Outer Membrane Protein G (Ompg) Nanopore Library: From The Discrimination Of Biotin-Binding Proteins In Serum To Resolving Human Carbonic Anhydrase From Human Red Blood Cells, 2019 University of Massachusetts Amherst
Building The Outer Membrane Protein G (Ompg) Nanopore Library: From The Discrimination Of Biotin-Binding Proteins In Serum To Resolving Human Carbonic Anhydrase From Human Red Blood Cells, Bib Yang
Doctoral Dissertations
The use of pore-forming proteins (PFPs) in nanopore sensing has been fruitful largely due to their nanoscale size and the ease with which protein nanopores can be manipulated and consistently reproduced at a large scale. Nanopore sensing relies heavily on a steady ionic current afforded by rigid nanopores, as the change in current is indicative of analyte detection, revealing characteristics of the analyte such as its relative size, concentration, and charge, as well as the nanopore:analyte interaction. Rigid PFPs have been used in applications such as DNA sequencing, kinetic studies, analyte discrimination, and protein conformation dynamics at the single-molecule level. …