Neuroprotective And Nootropic
Evaluation Of Myristica Malabarica On
Diabetes-Induced Cognitive Impairment
In Experimental Animals: Promising
For Controlling The Risk Of Alzheimer’S
Disease,
2022
Chulalongkorn University
Neuroprotective And Nootropic Evaluation Of Myristica Malabarica On Diabetes-Induced Cognitive Impairment In Experimental Animals: Promising For Controlling The Risk Of Alzheimer’S Disease, Kolguri Jagadeeshwar, Rajasekhar Reddy Alavala, Subhakar Raju Ravula, Umasankar Kulandaivelu, G.S.N. Koteswara Rao
The Thai Journal of Pharmaceutical Sciences
This study intends to explore the neuroprotective and memory-enhancing properties of Myristica malabarica (MM) leaf extracts on diabetic-induced cognitive decline rats. The models of Y maze and Morris water maze were selected for nootropic activity and neuroprotective properties were deliberate by assessing the levels of Ach, malondialdehyde (MDA), superoxide dismutase (SOD), nitric oxide (NO), catalase (CAT) and glutathione hormone (GSH) in diabetic rat brains. Significantly improved memory and learning with 100 mg/kg (P < 0.01) and 200 mg/kg (P < 0.001) of EAMM (L) and MAMM (L) in Y maze and Morris water maze models. Significant effect was observed in all the extracts with two doses when compared with diabetic control on day 75. The levels of AChE (P < 0.001), MDA (P < 0.01), and NO (P < 0.05) were decreased significantly and significantly raised (P < 0.01) levels of SOD, CAT, and GSH with MAMM (100 and 200 mg/kg) compared with disease control. Further studies on MM leaf are needed as evidence of cognitive impairment in defense against diabetes has been shown to expose its mode of action.
Cytotoxic Effect Of Crude Vitex Glabrata
Fruit Extracts On Human Cancer Cell Lines,
2022
Chulalongkorn University
Cytotoxic Effect Of Crude Vitex Glabrata Fruit Extracts On Human Cancer Cell Lines, Saraphorn Intarasam, Vorranuch Keerathinant, Mesayamas Kongsema, Kantimanee Pradermwong
The Thai Journal of Pharmaceutical Sciences
Vitex glabrata (VG) or Khai-noa fruit extracts were prepared from three methods; squeezing extract (SE), maceration extract (ME), and ethanol extraction (EE) to evaluate their effects in vitro. By the (1,1-diphenyl-2-picrylhydrazyl) scavenging assay, all extracts revealed very weak antioxidant. Results of (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide, a tetrazole) assay showed the increase in cell viability of SE at 100 and 500 μg/ml on TK6 cells after 24 and 48 h treatments (P < 0.01) without affecting Caco-2 cells. Conversely, a dose- and time-dependent cytotoxicity of all extracts revealed at 1000-2000 μg/ml on both cells (P < 0.01). SE showed cytotoxicity on PC-3 cells and anticancer activity when confirmed by clonogenic assay in a dose-dependent manner. SE also showed cytotoxicity on HT-29 cells after 24 h treatment but the cells could recurrent in the clonogenic assay. No effect was observed on MCF-7 cells after SE alone, SE co-treated with MMC treatment, and in the clonogenic assay. Moreover, HT-29 cells revealed a dose-dependent decrease in viability when treated with SE co-treated with MMC by 24 h. Hence, SE can selectively promote cell viability on TK6 and anticancer activity on PC-3. These results suggest that the different potential of SE depends on different cell lines.
In Situ Hyaluronic Acid-Based Hydrogel
Incorporated With Insulin-Like Growth Factor-1 For Three-Dimensional Encapsulation Of Keratinocytes,
2022
Chulalongkorn University
In Situ Hyaluronic Acid-Based Hydrogel Incorporated With Insulin-Like Growth Factor-1 For Three-Dimensional Encapsulation Of Keratinocytes, Pornsuda Lengwan, Pithi Chanvorachote
The Thai Journal of Pharmaceutical Sciences
Background/Aim: This work aimed to establish in situ hydrogel formation based on crosslinking of methacrylated hyaluronic acid (MeHA) and the polymer incorporated with insulin-like growth factor 1 (IGF-1) for keratinocyte three-dimension (3D) culture. Materials and Methods: The compatibility of human keratinocytes with developed hydrogel was determined by PrestoBlueTM cell viability assay. The live and dead cells were determined by Calcein AM, Propidium Iodide and Hoechst 33342 staining. The physical properties including gelation time, microstructure (porosity and interconnectivity), swelling ratio, and stability of the different concentration hydrogel were determined and correlated to cell viability. Results: Three percent w/v MeHA for in …
Fluid Properties Of Solvents And Oils
Used In In Situ Forming Microparticles,
2022
Chulalongkorn University
Fluid Properties Of Solvents And Oils Used In In Situ Forming Microparticles, Sai Myo Thu Rein, Torsak Intaraphairot
The Thai Journal of Pharmaceutical Sciences
In situ forming microparticle (ISM) has been recently used as the injectable drug delivery system prepared from polymeric non-aqueous phase in oil emulsion. Solvents and oils used in ISM including N-methyl pyrrolidone (NMP), 2-pyrrolidone (PYR), dimethyl sulfoxide (DMSO), glycofurol, triacetin, olive oil, camellia oil and isopropyl myristate (IPM) were evaluated for pH, density, viscosity, flow time, surface tension, contact angle, color distribution, injectability, antimicrobial activities and cytotoxicity. The most viscous camellia oil and olive oil showed the highest flow time with lowest color diffusion and lower injectability; thus, they were suitable for performing as external phase of ISM to prolong …
Formulation, Ex-Vivo And In-Vitro Characterization Of Liposomal Drug Delivery System Of Fexofenadine,
2022
Chulalongkorn University
Formulation, Ex-Vivo And In-Vitro Characterization Of Liposomal Drug Delivery System Of Fexofenadine, Safin Shrestha, Uttam Budhathoki
The Thai Journal of Pharmaceutical Sciences
In order to improve the low permeability associated with conventional or immediate release dosage forms such as nasal spray and oral solid dosage form, tablets, fexofenadine liposomes were prepared by using ethanol injection method by varying the concentration of surfactant, (Tween -80), along with crude lecithin and cholesterol. The optimized formulations was evaluated for size, shape, entrapment efficiency and in vitro drug release. In this study the optimized formulated liposomes appeared to be spherical in shape and particle size was found to be 4703.667±445 nm. There is 1.25 times more flux of liposome as compared with free drug. The entrapment …
Behavior Of Polymers And In-Vitro Evaluation On Gastroretentive Nifedipine Sustained Release Floating Tablets,
2022
Chulalongkorn University
Behavior Of Polymers And In-Vitro Evaluation On Gastroretentive Nifedipine Sustained Release Floating Tablets, H. K. Pradeep, Juturu Thimmasetty, P.R. Sathesh Babu, S. Gururaj, N. Sreeharsha
The Thai Journal of Pharmaceutical Sciences
Nifedipine is one of the calcium channel blockers widely employed in indications such as high blood pressure and angina pectoris. In the present study, a prompt attempt is done in the formulation of gastro retentive sustained-release tablets. The effect of polymers and their combination on release pattern, initial burst release, and floating behavior were studied. Out of four polymers, two polymers such as cellulose derivatives like highly viscous polymer Hydroxypropyl methylcellulose (HPMC) K100, low viscous HPMC K4M and their combinations, and other two polymers used were from natural sources like xantham gum, guar gum and their combinations were studied. The …
Metabolite Profiling And Activity Study
Of Ethanol Extract Of Chrysophyllum Cainito L. Leaves In Increasing Bone Density In Male Mice,
2022
Chulalongkorn University
Metabolite Profiling And Activity Study Of Ethanol Extract Of Chrysophyllum Cainito L. Leaves In Increasing Bone Density In Male Mice, Burhan Ma’Arif, Kurniawan Hidayat Perdana Putra, Miftah Saiful ‘Arifin, Rukiana, Reyhan Amiruddin, Weka Sidha Bhagawan, Arief Suryadinata, Fidia Rizkia Inayatilah, Roihatul Muti’Ah, Agnis Aditama
The Thai Journal of Pharmaceutical Sciences
Phytoestrogen is a group compound which has an estrogen-like structure or function. One of its roles was in the bone formation. Chrysophyllum cainito L. is a plant that contains phytoestrogen. The purpose of this research is to determine the metabolite profile and activity of1ethanolpextract of C. cainitoxleaves to increase trabecular femur bone density in male mice. The metabolite profiling analysis was done using ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry/mass spectrometry and analyzed using Masslynx 4.1 software. Four doses of ethanol extract of C. cainito leaves were prepared for treatment. Which are 2,14,48, and 316 mg/ body weight (BW) mice/day …
Acute And Sub-Acute Toxicity Profile Of Methanol Leaf Extract Of Geophila Obvallata On Renal And Hepatic Indices In Wistar Rats,
2022
Chulalongkorn University
Acute And Sub-Acute Toxicity Profile Of Methanol Leaf Extract Of Geophila Obvallata On Renal And Hepatic Indices In Wistar Rats, Osafanme Lucky Iserhienrhien, Paulinus Ngozi Okolie
The Thai Journal of Pharmaceutical Sciences
Introduction: Geophila obvallata (Rubiaceae) is a medicinal herb used for the treatment of diseases associated with oxidative damage. Objective: The study investigated the toxic effects of acute and sub-acute oral doses of methanol extracts of G. obvallata (GOE) in rats. Materials and Methods: In acute toxicity study, extract doses of 1600, 2900, and 5000 mg/kg were orally administered. The rats were observed for signs of toxicity for 2 weeks. In sub-acute toxicity study, the extract was administered to rats at doses of 100, 500, and 1000 mg/kg for 28 days, while control rats received only water. At the end of …
Preferences Of Thai Head Pharmacists For Pharmacy Technician Education: Certification Versus Bachelor’S Degree Program,
2022
Chulalongkorn University
Preferences Of Thai Head Pharmacists For Pharmacy Technician Education: Certification Versus Bachelor’S Degree Program, Sukunta Muadthong, Nusaraporn Kessomboon, Tsutomu Kitajima, Kamolnut Muangyim, Surachat Ngorsuraches, Pattapong Kessomboon
The Thai Journal of Pharmaceutical Sciences
Objective: This study aimed to investigate Thai hospital pharmacists’ preferences for a proposed bachelor’s degree curriculum for pharmacy technicians (PhTs), preparing PhTs to fill an advanced role in pharmacy, compared to the existing high vocational certificate (HVC) curriculum for PhTs. Methods: A labeled discrete choice experiment (DCE) questionnaire was sent by post to head pharmacists of 814 Thai Ministry of Public Health hospitals over a 2-month period. Respondents’ preferences for the proposed bachelor’s degree or the existing HVC of PhT were determined based on their selections among three attributes: Competency in pharmaceutical information services, competency in pharmaceutical inventory management, and …
Efavirenz Listed In The World Health Organization Prequalification Program: A Successful Case Study Of A Public Private Collaboration For Technology Transfer In Pharmaceutical Production And Quality Control In Thailand,
2022
Chulalongkorn University
Efavirenz Listed In The World Health Organization Prequalification Program: A Successful Case Study Of A Public Private Collaboration For Technology Transfer In Pharmaceutical Production And Quality Control In Thailand, Yupadee Sirisinsuk, Mukdavan Prakobvaitayakit, Nusaraporn Kessomboon, Usawadee Sutapuk, Kannikar Kijtiwatchakul, Noppawan Angkulsansanee
The Thai Journal of Pharmaceutical Sciences
Pharmaceutical technology transfer is an important tool for enhancing production development and quality control. The Government Pharmaceutical Organization (GPO) is the state enterprises under the Ministry of Public Health, whose main mission is to produce high quality medicines with reasonable price to increase accessibility to the Thai people. GPO has received the technology transfer from an overseas pharmaceutical company for the successful production of the efavirenz 600 mg tablet that was certified by the WHO Prequalification Program. The research objective was to study how this success has been achieved with the support of technology transference as well as the relevant …
The Susceptibility Of Penicillin,
Ceftriaxone, And Cefotaxime
Against Streptococcus Pneumoniae
Isolated From Patients With Invasive
Pneumococcal Diseases At A Teaching
Hospital In Thailand,
2022
Chulalongkorn University
The Susceptibility Of Penicillin, Ceftriaxone, And Cefotaxime Against Streptococcus Pneumoniae Isolated From Patients With Invasive Pneumococcal Diseases At A Teaching Hospital In Thailand, Jantima Traipattanakul, Sudaluck Thunyaharn, Juthathip Suphanklang, Wichai Santimaleeworagun
The Thai Journal of Pharmaceutical Sciences
Objective: Pneumococcal disease is a severe invasive infection causing morbidity and death, so the appropriate antimicrobials are greatly important. We aimed to determine the minimum inhibitory concentration (MIC) of penicillin, cefotaxime and ceftriaxone against S. pneumoniae. Methods: The clinical isolates were obtained from patients with invasive pneumococcal disease admitted to Phramongkutklao Hospital, Thailand over a period of 10 years between 2006 and 2015. The in vitro antimicrobial susceptibility testing of antibiotics including penicillin, ceftriaxone and cefotaxime against S. pneumoniae isolates were determined by E-test. The MIC range, MIC50, and MIC90 (μg/ml) and percentage of susceptible isolates were recorded. Results: Forty …
A Review On Ethnomedicinal And Phytopharmacological Potential Of Traditionally Wild And Endemic Plant Berberis Tinctoria Lesch.,
2022
Chulalongkorn University
A Review On Ethnomedicinal And Phytopharmacological Potential Of Traditionally Wild And Endemic Plant Berberis Tinctoria Lesch., Arumugam Vignesh, Ramamoorthy Sivalingam, Subramaniam Selvakumar, Krishnan Vasanth
The Thai Journal of Pharmaceutical Sciences
Introduction: Berberis tinctoria an evergreen shrub, endemic and predominantly found at a higher altitude of the Nilgiri Biosphere Reserve, India. This leaf and fruit are edible, which are also used in homeopathic remedies for countless illnesses. Objectives: B. tinctoria with diverse ethnomedicinal uses was focused in the prevailing study to detailed the phytochemical and pharmacological properties for further imminent research in this species. Materials and methods: Published data in this review were all gathered from the online bibliographical databases: PubMed, Elsevier, Scopus, Google Scholar, Web of Science, and local ethnic community peoples of Kurumba and Toda. Results: B. tinctoria was …
Gastroprotective Effect Of Phyllanthus
Reticulatus Poir. Against Pylorus Ligation-, Ethanol-Induced, And Stress-Induced Ulcer Models In Wistar Rats,
2022
Chulalongkorn University
Gastroprotective Effect Of Phyllanthus Reticulatus Poir. Against Pylorus Ligation-, Ethanol-Induced, And Stress-Induced Ulcer Models In Wistar Rats, Saravanan Jayaram, G. Thamotharan, N. Senthilkumar
The Thai Journal of Pharmaceutical Sciences
Objective: The objective of the present study is to evaluate the antiulcer property of ethanolic extract of Phyllanthus reticulatus Poir. against pylorus ligation-, ethanol-induced, and stress-induced ulcer models in Wistar albino rats. Materials and Methods: Three models – pylorus ligation-induced ulcer, ethanol-induced ulcer, and swim stress-induced ulcer – were used to induce ulcer in Wistar rats. The animals were treated with 200 mg/kg and 400 mg/kg p. o of the ethanolic extract of P. reticulatus Poir, to estimate the gastroprotective potential. The effect of P. reticulatus Poir. on pH of the gastric juice, volume of acid secretion, total and free …
Virgin Coconut Oil Ameliorates Arsenic Hepatorenal Toxicity And No-Mediated Inflammation Through Suppression Of Oxidative Stress In Rats,
2022
Chulalongkorn University
Virgin Coconut Oil Ameliorates Arsenic Hepatorenal Toxicity And No-Mediated Inflammation Through Suppression Of Oxidative Stress In Rats, Ademola C. Famurewa, Ekenechukwu K. Maduagwuna, Chinyere Aloke, Sharon O. Azubuike-Osu, Arunaksharan Narayanankutty
The Thai Journal of Pharmaceutical Sciences
Background: Compelling evidence has implicated oxidative stress as a leading mechanism for arsenic (As)-induced pathologies. We evaluated antioxidant effect of virgin coconut oil (VCO) against arsenic-induced oxidative stress-mediated hepatorenal damage in rats. Methods: Rats were randomly divided into three groups: Control group, as group, and VCO + As group. VCO was orally administered before and along with arsenic (10 mg/kg body weight, orally) for 21 days. Subsequently, serum liver enzymes, albumin (ALB), creatinine, urea, antioxidant enzymes, malondialdehyde, and nitric oxide (NO) level were evaluated. The histology of the tissues was analyzed with standard procedures. Results: Arsenic caused marked increases in …
Synthesis And Cytotoxic Activity Of N-(2,4-Dichloro)Benzoyl-N’-Phenylthiourea Against Human Breast Cancer Cell Line,
2022
Chulalongkorn University
Synthesis And Cytotoxic Activity Of N-(2,4-Dichloro)Benzoyl-N’-Phenylthiourea Against Human Breast Cancer Cell Line, Dini Kesuma, Anwar Medika, Tegar Achsendo Yuniarta
The Thai Journal of Pharmaceutical Sciences
Introduction: Urea- and thiourea-based compound has shown a potency to be further developed as anticancer compound. Objective: Another novel phenylthiourea analog (N-(2,4-dichloro)benzoyl-N’-phenylthiourea) was synthesized through Schotten-Baumann reaction followed by the assessment of its cytotoxic activity. Methods: N-(2,4-dichloro)benzoyl-N’-phenylthiourea was synthesized using N-Phenylthiourea and 2,4-dichlorobenzoyl chloride as starting materials. The reaction started at low temperature for 30 min followed by reflux for 8 h to yield target compound. Cytotoxicity assay was then performed against MCF-7, T47D, and Vero normal cell line. Results: The compound has been synthesized and its structure has been verified using infrared, 1H-nuclear magnetic resonance (NMR), 13C-NMR, various 2D …
Gc-Ms Fingerprints Combined With Chemometric Analysis For The Authentication Of Morus Alba Leaves From Thailand,
2022
Chulalongkorn University
Gc-Ms Fingerprints Combined With Chemometric Analysis For The Authentication Of Morus Alba Leaves From Thailand, Malai Satiraphan, Aye Thida, Lawan Sratthaphut, Patcharawan Tanamatayarat, Onoomar Toyama
The Thai Journal of Pharmaceutical Sciences
Introduction: Morus alba L. is one of the economic plants in Thailand. Several varieties are grown in various parts of the country. Different varieties from different regions may produce dissimilar chemical fingerprints. Objectives: The gas chromatography coupled to mass spectrometry (GC-MS) fingerprints of 2 varieties of M. alba leaves from three locations in Thailand, along with tea products will be established. Materials and Methods: The GC-MS chromatograms combined with chemometric analysis were used to analyze the extracts of M. alba leaves and their tea products. Results: A total of 83 compounds comprising terpenes, saturated fatty acids, unsaturated fatty acids and …
Screening Of Process Variables Using Box–Behnken Design In The Fabrication Of Berberine Hydrochloride-Loaded Transethosomes For Enhanced Transdermal Delivery,
2022
Chulalongkorn University
Screening Of Process Variables Using Box–Behnken Design In The Fabrication Of Berberine Hydrochloride-Loaded Transethosomes For Enhanced Transdermal Delivery, Koushlesh Kumar Mishra, Chanchal Deep Kaur
The Thai Journal of Pharmaceutical Sciences
Objective: The objective of the present work was to develop, optimize, and characterize berberine hydrochloride (BBN-HCl)-loaded transethosomes (TEs) for enhanced transdermal delivery. In this study, screening of formulation and process variables was conducted using Box–Behnken design approach to observe significant and insignificant influence on the TEs. Materials and Methods: The TEs was developed by homogenization technique (hot method). The optimized BBN-HCl-loaded TEs were evaluated for its vesicle size (VS), polydispersity index (PDI), zeta potential (ZP), loading capacity, and entrapment efficiency. Characterization was done by powder X-ray diffraction (P-XRD), differential scanning calorimetric (DSC), and transmission electron microscopy. Further, in vitro drug …
Analysis Of Pharmaceutical Inventory Management In A State Hospital In Myanmar,
2022
Chulalongkorn University
Analysis Of Pharmaceutical Inventory Management In A State Hospital In Myanmar, Mya Pann Thazin, Rungpetch Sakulbumrungsil
The Thai Journal of Pharmaceutical Sciences
Inventory management analysis for a state-level hospital pharmacy of Myanmar was conducted using ABC-VEN matrix analysis to identify the drug categories that require stringent management control. The annual expenditure of all drug items managed by pharmacy department for the years 2016 and 2017 was analyzed and application of ABC, VEN, and ABC-VEN matrix analysis was performed. The study showed that the average annual expenditure was 288,414 USD. Of the total 297 items of medicines, 7.7% (23), 16.8% (50), and 75.4% (224) were classified as A, B, and C category items, respectively, accounting for 70.4%, 19.7%, and 9.9% of drug expenditure. …
Chloroquine And Eye Problems At A General Hospital In Western Thailand,
2022
Chulalongkorn University
Chloroquine And Eye Problems At A General Hospital In Western Thailand, Nunthaluxna Sthapornnanon, Chattraporn Chumchit, Supakarn Suksajjee, Sukanya Boonnum, Suphitchaya Neramitwanchai, Chitthathep Chaisaen
The Thai Journal of Pharmaceutical Sciences
Objective: This study aimed to explore the eye problems after CQ use, to review if the eye examinations were performed during patients were treated with CQ and to identify factors related to those eye problems. Method: Retrospective study was done. Data were collected from both electronic medical records and medical records. Patients who were treated with CQ during May15, 2010- May 31, 2019 at a general hospital in western Thailand were included. Results: There were 123 patients who were treated with CQ. Eye problems were found in 26 patients (21.14 %). Majority of those had blurred vision (13/26; 50.00%). Focus …
Recent Advancements On Biological Activity Of Indole And Their Derivatives: A Review,
2022
Chulalongkorn University
Recent Advancements On Biological Activity Of Indole And Their Derivatives: A Review, Punet Kumar, Md Iftekhar Ahma, Sangam Singh, Mohammad Rizki Fadhil Pratam, Arun K. Mishra
The Thai Journal of Pharmaceutical Sciences
Indole, a versatile outstanding heterocyclic compound, engaged in numerous pharmacological properties due to their multiple biochemical processes. The remarkable indole moiety resembles with numerous protein structures. The fascinating molecular framework of indole makes its suitable for drug development. Indole derivatives mimic the peptides structure and bind reversibly to several enzymes, which contribute enormous opportunities to develop novel drugs with distinct mechanism of action. The presence of nitrogen-based heterocycles in several compounds has been investigated enormously as the core structures comprise several biologically appropriate molecules found active in different diseases. The investigations conducted worldwide have shown outstanding impact for scientists, working …
