Δ-9-Thc Effect On The Dopamine Transporter, 2018 CUNY John Jay College
Δ-9-Thc Effect On The Dopamine Transporter, Brooke E. Nielsen
Understanding the mechanism behind drugs of abuse is one of the key elements to understanding why addiction is so prevalent in society. The goal of this research was to determine the effect that a popular cannabinoid, Δ -9-tetrahydrocannabinol has on the regulation of dopamine neurotransmitter in the synapses. It was hypothesized that the addictive high users experience from using Δ-9-THC had a direct effect on lowering the dopamine transporter protein on cell surface causing a buildup of dopamine in the synapse. The results of the study show that the higher concentration of Δ-9-THC that the cells were exposed to caused ...
Zolpidem Facilitated Sexual Assaults: A Hair Method Validation, 2018 CUNY John Jay College
Zolpidem Facilitated Sexual Assaults: A Hair Method Validation, Danielle N. Chasworth
A liquid chromatography tandem mass spectrometry (LC-MSMS) method was developed and validated to detect for the presence of zolpidem in human hair samples. Zolpidem is a sedative hypnotic whose adverse effects make it a common drug found in drug facilitated crimes (DFC). It is important to be able to detect and quantitate the drug after a long period of time due to the victim’s delayed reporting of the crime. Hair’s long window of detection makes it a useful matrix for DFC investigations. The linear range of the assay was targeted at 2 pg/mg to 1,000 pg ...
Radical Social Ecology As Deep Pragmatism: A Call To The Abolition Of Systemic Dissonance And The Minimization Of Entropic Chaos, Arielle Brender
Student Theses 2015-Present
This paper aims to shed light on the dissonance caused by the superimposition of Dominant Human Systems on Natural Systems. I highlight the synthetic nature of Dominant Human Systems as egoic and linguistic phenomenon manufactured by a mere portion of the human population, which renders them inherently oppressive unto peoples and landscapes whose wisdom were barred from the design process. In pursuing a radical pragmatic approach to mending the simultaneous oppression and destruction of the human being and the earth, I highlight the necessity of minimizing entropic chaos caused by excess energy expenditure, an essential feature of systems that aim ...
Examining The Roles Of Genetic And Environmental Factors In Drosophila Melanogaster Hematopoiesis And Innate Immune System, Minkyung Lee
Nearly 24 million people are affected by autoimmune diseases in the United States. Main causes of autoimmune diseases have been attributed to genetic predisposition and environmental exposure to chemicals such as hormones and pesticides. Due to the large population that are affected by autoimmune diseases, it is critical to understand the mechanisms behind them. In this study, we sought to explore both genetic and environmental factors that affect hematopoiesis, or the formation of specific blood cells, and immune system in Drosophila melanogaster. As Drosophila melanogaster have conserved pathways of hematopoiesis as humans, they were used as the model organism for ...
Manganese Toxicity In The Dopamine Synthesis Pathway In Drosophilia Melanogaster, 2018 Carroll College - Helena
Manganese Toxicity In The Dopamine Synthesis Pathway In Drosophilia Melanogaster, Brandon J. Adair, Tamra E. Jones
Carroll College Student Undergraduate Research Festival
Manganese is prevalent in Montana, and Manganese toxicity symptomatically resembles Parkinson’s disease. The goal of this project was to determine whether exposure to high levels of Manganese affects the Dopamine synthesis pathway. For our experiment, we chose to measure the expression of the ple gene in Drosophila melanogaster that were exposed to Manganese during development. The ple gene codes for Tyrosine Hydroxylase, an enzyme that functions in the first rate-limiting step of Dopamine synthesis. We hypothesized that an abundance of Manganese would result in decreased expression of ple in larvae and observable motor function deficits among adult flies. In ...
Effects Of Everyday Toxin, Titanium Dioxide, On Drosophila Melanogaster Nervous System Development, 2018 Carroll College, Helena, MT
Effects Of Everyday Toxin, Titanium Dioxide, On Drosophila Melanogaster Nervous System Development, Maria Carparelli, Kelsey Ripley
Carroll College Student Undergraduate Research Festival
Titanium dioxide (TiO2) is a widely used compound found in everything from food packaging to sunscreens. When ingested, TiO2 is readily transported across membranes and efficiently stored within cells. Previous studies showed that exposure to TiO2 results in underdeveloped nervous systems. For our study, we attempted to answer the following question: Will exposing Drosophila melanogaster larvae to TiO2 affect expression of the Neur gene and development of the central nervous system? The Neur gene is crucial during the cell-determination stage of development as its encoded protein helps specify neuroblast development and aids in nervous system and sensory organ development. It ...
Evaluating The Aryl Hydrocarbon Receptor As A Target For Pharmacologic Activity Of Repurposed Drugs, 2018 University of New Mexico
Evaluating The Aryl Hydrocarbon Receptor As A Target For Pharmacologic Activity Of Repurposed Drugs, Teofilo Borunda Duque
Pharmaceutical Sciences ETDs
The discovery of new pharmacologic targets is important for the advancement of pharmacotherapy and identification of new indications for current drugs. The aryl hydrocarbon receptor (AHR) is a physiologic sensor of both chemical environmental pollutants and ligands of natural origin. Given the broad spectrum of ligands that activate the AHR and its relationship with toxicology, the AHR is not thought to be a traditional target for pharmacotherapy. However, multiple studies have shown potential for the AHR as a novel pharmacologic target Therefore, identifying less toxic agents that modulate the AHR may elucidate mechanisms for pharmacological targeting of the AHR. The ...
The Effects Of Uv Radiation On Metarhizium Anisopliae, 2018 Carroll College, Helena, MT
The Effects Of Uv Radiation On Metarhizium Anisopliae, Victoria Hill
Life and Environmental Sciences Undergraduate Theses
The development of fungal insecticides as biological control agents provides a safer, more natural approach to pest control than chemical insecticides. Metarhizium anisopliae, one of the most widely used mycoinsecticides, is one fungal species of high economic potential in current use. However, a rapid decrease in pathogen activity in the field due to ultraviolet radiation presents a problem for efficacy and further adoption by farmers. The present study seeks to determine if certain strains of M. anisopliae are more tolerant of UV radiation, thus providing the potential for higher effectiveness in the field. Utilizing a novel method for examining fungal ...
Mitigation Of Livestock Odors Using Black Light And A New Titanium Dioxide-Based Catalyst: Proof-Of-Concept, 2018 Iowa State University
Mitigation Of Livestock Odors Using Black Light And A New Titanium Dioxide-Based Catalyst: Proof-Of-Concept, Wenda Zhu, Jacek A. Koziel, Devin L. Maurer
Jacek A. Koziel
Concentrated livestock feeding operations have become a source of odorous gas emissions that impact air quality. Comprehensive and practical technologies are needed for a sustainable mitigation of the emissions. In this study, we advance the concept of using a catalyst for barn walls and ceilings for odor mitigation. Two catalysts, a new TiO2-based catalyst, PureTi Clean, and a conventional Evonik (formerly Degussa, Evonik Industries, Essen, Germany) P25 (average particle size 25 nm) catalyst, were studied for use in reducing simulated odorous volatile organic compound (VOC) emissions on a laboratory scale. The UV source was black light. Dimethyl disulfide (DMDS), diethyl ...
Development Of An Automated Method For Selected Aromas Of Red Wines From Cold-Hardy Grapes Using Solid-Phase Microextraction And Gas Chromatography-Mass Spectrometry-Olfactometry, 2018 Iowa State University and DuPont Crop Protection
Development Of An Automated Method For Selected Aromas Of Red Wines From Cold-Hardy Grapes Using Solid-Phase Microextraction And Gas Chromatography-Mass Spectrometry-Olfactometry, Lingshuang Cai, Somchai Rice, Jacek A. Koziel, Murlidhar Dharmadhikari
Jacek A. Koziel
The aroma profile of red wine is complex and research focusing on aroma compounds and their links to viticultural and enological practices is needed. Current research is limited to wines made from cold-hardy cultivars (interspecific hybrids of vinifera and native N. American grapes). The objective of this research was to develop a fully automated solid phase microextraction (SPME) method, using tandem gas chromatography-mass spectrometry (GC-MS)-olfactometry for the simultaneous chemical and sensory analysis of volatile/semi-volatile compounds and aroma in cold-hardy red wines. Specifically, the effects of SPME coating selection, extraction time, extraction temperature, incubation time, sample volume, desorption time ...
Regulation Of Radioactive Fracking Waste, 2018 CUNY School of Public Health
Regulation Of Radioactive Fracking Waste, Elizabeth Ann Glass Geltman, Nichole Leclair
Publications and Research
Natural gas extracted form shale reached record production totals in 2015 in the United States and the US Energy Information Administration (EIA) forecasts natural gas production will continue to increase. Wastes from shale gas extraction can contain the radioactive isotopes radium-226 (Ra-226) and radium-228 (Ra-228), which decay further into radon (Rn). Exposure to radon, a form of naturally occurring radioactive materials (NORM), is the leading cause of lung cancer in the United States, after smoking. This article explores how states handle the disposal of technologically enhanced naturally occurring radioactive materials (TENORM) and/or NORM waste from oil and gas operations ...
The Forensic Characterization Of Bacterial And Fungal Organisms In Traditional Chinese Medicine, 2018 Virginia Commonwealth University
The Forensic Characterization Of Bacterial And Fungal Organisms In Traditional Chinese Medicine, Julia Grzymkowski, Christopher J. Ehrhardt, Justin L. Poklis, Michelle R. Peace
Undergraduate Research Posters
There has been an increase in use of Traditional Chinese Medicine (TCM) in the United States because they are less expensive and believed to be more effective with less adverse effects in comparison to traditional pharmaceutics. Therefore, sales have increased in the US, despite articles and case studies demonstrating the dangers, such as injury and death, related to TCM, stemming from improper labelling, toxic contaminants, and, in some cases, the presence of pathogenic bacteria. The aim of this study was to perform a survival experiment to demonstrate the importance of proper herbal brewing technique and to conduct a molecular and ...
Fate Of Methoprene In Temperate Salt Marsh Ditches Following Aerial Applications, 2018 Department of Technology and Society
Fate Of Methoprene In Temperate Salt Marsh Ditches Following Aerial Applications, David J. Tonjes, Anne E. Mcelroy, Robin K. Barnes, Dominick V. Ninivaggi, Walter Dawydiak, Gregory T. Greene, Bruce J. Brownawell
Technology & Society Faculty Publications
Aerial applications of liquid methoprene are used in salt marshes to control mosquitoes by preventing adult emergence. Despite concern about toxicity to non-target organisms, little is known about environmental concentrations after applications, nor methoprene's persistence in salt marsh environments. Aqueous and sediment samples were collected from two marshes receiving weekly applications. Aqueous samples were collected as early as 30 minutes after applications and as long as nine days afterwards; sediment samples were taken within hours of application and as long as 19 days post-application. Use of time-of-flight liquid chromatography – mass spectral analysis allowed for ultra low detection limits (0 ...
The Combined Effects Of Atrazine And Tetracycline On Primary Producers And Zooplankton In Freshwater Microcosms, 2018 Georgia Southern University
The Combined Effects Of Atrazine And Tetracycline On Primary Producers And Zooplankton In Freshwater Microcosms, Madison R. Kelly
University Honors Program Theses
Widespread use of agrochemicals such as herbicides and antibiotics increases their likelihood of entering aquatic systems in mixture. Despite different modes of action, atrazine (herbicide) and tetracycline (antibiotic) adversely affect non-target photosynthetic organisms, such algae and macrophytes, with the potential to reduce food availability to higher trophic levels. However, the effects of simultaneous exposure to both contaminants have yet to be determined. I hypothesized that a mixture of atrazine and tetracycline affects freshwater communities differently than each compound alone. A microcosm experiment was conducted to test effects of environmentally relevant concentrations of atrazine (1 μg L-1) and tetracycline (1 ...
Fumonisin B1 In Bt And Non-Bt Maize: A Meta-Analysis, 2018 Iowa State University
Fumonisin B1 In Bt And Non-Bt Maize: A Meta-Analysis, Kayla Cappelle
Graduate Theses and Dissertations
Fumonisins are a class of mycotoxins that are of interest due to their occurrence in feed and food grains at concentrations which may be adverse to the health of humans and animals. The presence and mitigation of fumonisin has been a topic of widespread study, but the meta-effect of Bt maize as a method of mitigation has not been fully established in terms of the probable incidence and magnitude of mitigation achieved regionally over time.
A systematic review of agronomic data and subsequent meta-analysis were used to develop a more complete understanding of fumonisin B1 (FB1) levels regionally over several ...
Optimization Of Vegetative Filter Strips For Mitigation Of Runoff From Golf Course Turf, 2018 University of Massachusetts Amherst
Optimization Of Vegetative Filter Strips For Mitigation Of Runoff From Golf Course Turf, Barbara Deflorio
OPTIMIZATION OF VEGETATIVE FILTER STRIPS FOR MITIGATION OF RUNOFF FROM GOLF TURF
BARBARA A. DEFLORIO, B.S., UNIVERSITY OF MASSACHUSETTS AMHERST
Directed by: Professor John M. Clark
Many pesticides are listed as possible or probable human carcinogens, leading to a public concern over their environmental impact. Vegetative filter strips (VFS) have been developed to intercept runoff water and prevent pesticides from contaminating surface and ground water.
The first half of this project identified five plants (big blue stem, blue flag iris, eastern gama grass, prairie cord grass and wool grass) that best removed pesticides from contaminated soil ...
Organizational Effects Of Defeminizing Toxicants: Lessons Learned From An Environmental Sentinel Organism, The Fathead Minnow., 2017 University of Nebraska Medical Center
Organizational Effects Of Defeminizing Toxicants: Lessons Learned From An Environmental Sentinel Organism, The Fathead Minnow., Jonathan Ali
Theses & Dissertations
Endocrine disrupting compounds (EDCs) are chemicals that interfere with hormone function and are increasingly detected in aquatic environments, where they elicit adverse effects from exposed organisms. The toxicological effects of EDCs can be described as either activational (reversible) or organizational (irreversible), where the latter are associated with adverse outcomes in reproductive performance of adult fish. However, few studies have investigated the organizational impacts of anti-estrogenic or “defeminizing” EDCs, e.g. agrichemicals or pharmacological agents, in an environmentally-relevant or “sentinel” species. The objective of this study was to investigate the impacts of early-life EDC-initiated changes in estrogenic gene expression on organizational ...
Identification And Quantification Of Cyanide And Its Metabolites In Lemur Urine, 2017 City University of New York (CUNY)
Identification And Quantification Of Cyanide And Its Metabolites In Lemur Urine, Jeremy Peralta
Cyanide is a chemical compound that contains the monovalent groups of carbon and nitrogen. This substance is considered a rapidly acting deadly chemical. However, some animals such as lemurs consume food products that are known to contain elevated levels of cyanide without major consequences. The mechanism by which the lemur can handle this high exposure to cyanide is unknown. In this study, we developed and validated two analytical methods for the determination of cyanide and two metabolites, thiocyanate and 2-aminothiazoline-4-carboxylic acid (ATCA) in lemur urine. The method was applied to 47 authentic lemur urine samples collected from 4 different species ...
Age-Related Host Factors Regulate Lung Cancer Progression., 2017 University of Louisville
Age-Related Host Factors Regulate Lung Cancer Progression., John Caleb Greenwell
Electronic Theses and Dissertations
Cancer is largely a disease of the elderly. In the United States alone, 87% of all cancers in 2017 will be diagnosed in individuals aged 50 years or older. Among them, lung cancer is the deadliest, accounting for 1 in 4 cancer deaths. The 5-year survival rate is below 20%, a number which has not changed substantially over the past several decades. This underscores a desperate need for new strategies in prevention, early detection and treatment of this deadly disease. While tobacco use is unquestionably the number one risk factor for the development of lung cancer, the majority of diagnoses ...
Multigeneration Effects Of Chronic Exposure To Polycyclic Aromatic Hydrocarbons In Gulf Killifish (Fundulus Grandis), 2017 Louisiana State University at Baton Rouge
Multigeneration Effects Of Chronic Exposure To Polycyclic Aromatic Hydrocarbons In Gulf Killifish (Fundulus Grandis), Andrea Yammine
LSU Master's Theses
The goal of this study was to examine potential adaptation within two generations of Gulf killifish exposed to polycyclic aromatic hydrocarbons (PAH) while also investigating the use of mucus as an effective and non-lethal alternative to determine concentrations of 11-ketotestosterone (11-KT) and vitellogenin (VTG) of F. grandis. Multiple biomarkers were used to assess adaptation within these fish, such as lysozyme (enzyme critical in innate immunity), and 11-ketotestosterone and vitellogenin (endocrine hormones often affected by PAHs).
Mucal concentrations of 11-KT and VTG were determined and evaluated for effectiveness through comparative assays with blood plasma. This method was then applied to evaluate ...