Life Sciences Commons^™

Mixture Design Response Surface Methodology Analysis Of Seven Natural Bioactive Compounds To Treat Prostate Cancer, Ian Geddes Berlin

Theses and Dissertations

Natural bioactive compounds have drawn the interest of many researchers worldwide in their effort to find novel treatments, including prostate cancer (PC) treatment which is estimated to be 13.1% of all new cancer cases in the U.S. in 2021. Many of these bioactive compounds have been identified from treatments in traditional Chinese medicine (TCM), that often have multiple bioactive compounds present. However, in vitro studies frequently focus on the compounds in isolation, or in simple combinations of two compounds. We used mixture design response surface methodology (MDRSM) to assess changes in PC cell viability after 48 hours of treatment to …

Elucidating The Structural And Dynamical Properties Of The Intrinsically Disordered Protein Nrf2 Using Molecular Dynamics Simulations, Megan Nicole Chang

Electronic Thesis and Dissertation Repository

The nuclear factor erythroid 2-related factor 2 (Nrf2) protein is a critical transcription factor for activating the antioxidant response pathway, a primary defense mechanism against disproportionate levels of oxidants in the cell, via the upregulation of cytoprotective genes. Notably, aberrant activation of Nrf2 in cancer cells increases their resistance to chemotherapy, rendering the treatment ineffective. The focus was to uncover the conformational landscape of Nrf2’s Neh4 and Neh5 domains, which participate in crucial interactions for complete transcriptional activation. Since Nrf2 is an intrinsically disordered protein (IDP), molecular dynamics simulations were employed to capture its dynamic nature and conformational heterogeneity. The …

Investigating Mycelial-Crop Residue Mat Application To Reduce Early-Colonizing Weeds In Row-Crop Agriculture, Donald T. Watson

Electronic Thesis and Dissertation Repository

Herbicide use within conventional agriculture has contributed to greatly increased crop yields since its widespread adoption, but environmental concerns regarding overuse and reliance on selective herbicides continue to mount. Using five fungal species and two crop residues in a factorial design, I created a novel slurry to control weeds through inhibition by the mycelial mat formed after application to soil. I monitored weed stem counts and the strength of the mycelial mat under the treatments. Additionally, as a proxy for crop yield, I measured the wet and dry mass of crop plant grown under application treatments. Weed prevalence was significantly …

Investigating The Role Of Pkc And Its Mechanisms In Regulation Of Igf-I Bioavailability In Fetal Growth Restriction, Allan W. Chen

Electronic Thesis and Dissertation Repository

Fetal growth restriction (FGR) is associated with decreased nutrient availability and reduced insulin-like growth factor (IGF)-I bioavailability via increased IGF binding protein (IGFBP)-1 phosphorylation. While protein kinase C (PKC) is implicated in IGFBP-1 hyperphosphorylation in nutrient deprivation, the mechanisms remain unclear. I hypothesized that the interaction of PKCα with protein kinase CK2β and activation of PKCα under leucine deprivation (L0) mediate fetal hepatic IGFBP-1 hyperphosphorylation. Parallel Reaction Monitoring Mass Spectrometry (PRM-MS) followed by PKCα knockdown demonstrated the PKCα isoform interacts with IGFBP-1 and CK2β under L0. Pharmacological PKCα activation with phorbol 12-myristate 13-acetate (PMA) increased whereas inhibition with bisindolylmaleimide II …

Impact Of Theory-Based Interventions On Meaningfulness Of Undergraduate Nursing Pharmacology Student Learning, Betsy L. Mauldin

Nursing Theses and Dissertations

Objective: Nursing pharmacology is a complex and content-dense course, often cited as difficult for nurse educators and students and a reason for not progressing in nursing curricula. Even nurses who experience high academic performance report feeling ill-prepared to adequately perform medication management for nursing practice in the current fast-paced environment. The objective of this five-chapter portfolio is to explain the development and implementation of a two-pronged intervention to facilitate meaningful learning of pharmacology principles within nursing students. Chapter 1 describes an overview of the approach to the intervention.

Methods: Chapter 2 describes one prong of the study intervention, the Pharmacology …

The Transfer Of Motor Skill Practice To The Game: A Review Of Transfer Of Learning Theory And Applications, Silver Dawson

Honors Theses

Abstract

Transfer of learning from practice to competition is a prevalent issue that needs the assistance of professionals in motor behavior and coaching education. This review provides a theoretical and applied overview of transfer of learning and practice design. Lacking the appropriate knowledge and skillset to create an effective practice design does not have to be common for coaches. Athletes and coaches need more evidence of transfer of learning behind their practice design and assessment. This research review gives support to theories and concepts like Fitts and Posner's stages of learning, specificity of learning, schema theory, verbal cues augmented feedback, …

Understanding The Kinomic Contributions To Tyrosine Kinase Inhibitor Resistance In Triple Negative Breast Cancer, Cory Lefebvre

Electronic Thesis and Dissertation Repository

Resistance to tyrosine kinase inhibitors (TKIs) presents a growing challenge in the development of therapeutic targets for cancers such as triple negative breast cancer (TNBC), where conventional therapies are ineffective at combatting systemic disease. Potential targets in TNBC include the receptor tyrosine kinases EGFR (epidermal growth factor receptor) and c-Met, however, targeted anti-EGFR and anti-c-Met therapies have faced challenges in clinical trials due to acquired resistance. We hypothesize that response versus resistance of triple negative breast cancer to the tyrosine kinase inhibitors erlotinib and cabozantinib is mediated by compensatory changes in the kinome and phosphoproteome. To test this, we (1) …

Hibernation Is Super Complex: Dynamics Of Electron Transport System Supercomplexes, Amalie J. Hutchinson

Electronic Thesis and Dissertation Repository

The electron transport system complexes form supercomplexes (SCs) within mitochondrial membranes, perhaps increasing respiratory capacity or reducing reactive oxygen species production. My project aimed to determine the abundance, composition, and stability of SCs in a hibernator. Hibernators have dynamic metabolisms that change greatly during the winter. I isolated mitochondria from rats and thirteen-lined ground squirrels (TLGS) in different hibernation states and measured mitochondrial respiration. I extracted mitochondrial proteins using two detergents of different strengths, and quantified SC abundance using 2-dimensional gel electrophoresis and immunoblotting. Rats had fewer SCs than TLGS. SCs are dynamic in hibernation and the complex III composition …

Dolphins In Ny? Researchers Turn To Boat Tours For Sightings, Aaron K. Tremper

Capstones

Before 2009, coastal bottlenose dolphins were a rare sight north of New Jersey. According to local researchers, large pods of bottlenose dolphins made their way that spring/summer along Long Island. While dolphin population data by state and federal wildlife regulators are spotty, studies by local scientists suggest more dolphins have been flocking to New York since 2009. Many researchers are turning to whale-watching ventures to learn more about what dolphins are up to in the Big Apple.

https://jkt711.github.io/capstone/

Lost At Sea, Anny Oberlink

Capstones

At the end of World War I and World War II, in a new era of peace, nations confronted an unprecedented logistical problem: millions of tons of unexploded ordnance—once a wartime boon—had become a peacetime burden. Faced with a mandate to dispose of excess munitions, militaries turned to dumping their stockpiles into the sea. But now a complex and urgent issue is emerging. Increasingly, as industry looks to build offshore—wind power turbines, internet cables, oil pipelines—they are facing a potential peril: millions of tons of unexploded bombs and ammunition that are lying on the ocean floor can explode or leak …

Effect Of Music On Rating Of Perceived Exertion And Duration Of Physical Activity, Mia Hines

Honors Projects

This study addresses the relationship between listening to music and physical activity.

Paralyzed Within A Plastic World, Gena Leib

Honors Projects

Our oceans are one of the largest ecosystems found on our planet, and they are also one of the driving forces to a balanced planet. I believe that it is very important to restore our oceans to the once clean environment, and it is up to mankind to make that first step to do so soon and without hesitation. I believe that many people are uniformed on the topics of pollution, and it is important to bring attention to the issues through a perspective that society can see. Social media has had a huge influence on helping this, but for …

Water Color: Inspiring Conservation Of Freshwater Ecosystems By Painting With Algae, Kate Lochridge

Honors Projects

“Water Color: Inspiring Conservation of Freshwater Systems by Painting With Algae” is a project that focuses on raising awareness of harmful algal blooms in Lake Erie through the creation of algae-based paint. Its goal is to provide a multidisciplinary outlet for understanding complex interactions between the causes, effects, and solutions of Harmful Algal Blooms (HABs). The novel part of this project is the creation of watercolor paint from algae cells. This paint was used to create algae-based artwork that facilitates an aesthetic interaction with the science of HABs when other informative materials- such as journal articles- can be confusing and …

Antimicrobial Activity Of Bacterial Virus Components: An Empirical Investigation Of The Killing Capacity Of Toxins From Burkholderia, Kyle Walny

Honors Projects

Given the growing issue in healthcare of antibiotic resistance, effective and safe alternative treatment methods are required. One of these possible alternative treatment methods is bacteriotoxins including bacteriocins and tailocins. The focus of this study is a bacteriotoxin from Burkholderia cenocepacia (ATCC 25608), which was induced for toxin using a modified UV light induction procedure and tested against a variety of Pseudomonas and Burkholderia for its killing capacity. Various other pathogenic strains were then induced with UV light and tested. The results showed that the toxin from ATCC 25608 was very effective against most of the Burkholderia tested and warrants …

How Can Stem Retention Rates At Bgsu Be Increased Both In And Out Of The Classroom?, Josalyn A. Coffee

Honors Projects

Previous research in STEM student retention rates showed a gap in knowledge regarding why this group of student experience higher rates of burnout than other groups of students. This study investigated the barriers STEM students at BGSU experienced. A survey was sent out to STEM and non-STEM students alike that asked questions regarding student’s personal history before attending BGSU, their course experiences at BGSU, and the levels of support they have outside of BGSU. The survey data showed that there was little difference in the rates that students switch majors solely based on being in the STEM field. The data …

Sex- And Age - Specific Migratory Strategies Of Blue Whales In The Northeast Pacific, Christina Blevins

Biology ETDs

Sequential subsampling of blue whale baleen plates can yield information of individual migratory plasticity of this endangered species in the northeast Pacific Ocean. We measured δ13C and δ15N isotope values along baleen from thirteen whales of different age and sex. Results showed sex/age-specific migratory strategies. Adult females exhibited relatively stable cyclical movements between temperate and subtropical regions. Adult males exhibited two strategies, most remained within temperate latitudes, whereas two migrated to subtropical latitudes. Movement patterns in juveniles were erratic. These patterns are potentially driven by energetic requirements of females, intraspecific competitions among adult males, and inexperience in locating prey in …

Seasonal Flammability Comparisons Of Native And Exotic Plants In The Post Oak Savannah, Blackland Prairie, And Pineywoods Ecoregions Of Texas, Michael Tiller

Electronic Theses and Dissertations

East Texas’ diverse landscape can present year-round wildfire seasons that can be influenced by seasonal and regional differences in climate and physiography. Greater insight into the fundamental thermal behavior of wildland fuels can aid in fire behavior prediction and development of fire-resistant plant lists. This study focused on estimating seasonal and regional flammability characteristics of five evergreen species: yaupon (Ilex vomitoria), Chinese privet (Ligustrum sinense), greenbrier (Smilax spp.), eastern red cedar (Juniperus virginiana), and escarpment live oak (Quercus fusiformis); and two deciduous species: Chinese tallow (Triadica sebifera) and southern …

Examining The Need For Parental Satisfaction With The Msu Extension Equine Assisted Therapy Program, Ashlyn Ann Kiker

Theses and Dissertations

Animal assisted interventions are becoming more prevalent in our society. Animal interventions have become a popular therapy technique that exist to aid a variety of disabilities. Equine therapeutic riding has become one of the most popular forms of animal assisted therapy for both children and adults. While research shows the importance and benefits of animal assisted therapy for children, there is a lack of research concerning parental satisfaction within therapeutic riding programs.

This study tested a newly developed instrument for measuring parental satisfaction for therapeutic riding programs. The Parent/Caregiver Evaluation Tool (Kerr, 2021) was administered to parents/caregivers whose children participated …

The Effects Of Hydrologic Heterogeneity On Harmful Algal Blooms In Freshwater Reservoir, Lake Sinclair, Georgia, Margaret Blackledge

Biology Theses

Aquatic habitats are frequently studied after a major water quality problem like the occurrence of an algal bloom. In this study, A proactive rather than a reactive response was considered, where the complexity of conditions conducive to uncontrolled cell growth were studied before a bloom took place by sampling regularly. This study aimed to monitor water quality by monthly sampling of algal communities for approximately one year. As the base of the aquatic food web, algae are a highly diverse group of organisms with varying sensitivity to physical and chemical changes in the environment. Four shallow sites were monitored at …

Phylogeny And Visual System Evolution In Sap Beetles (Coleoptera: Cucujoidea: Nitidulidae), Gareth S. Powell

Theses and Dissertations

Coleoptera is currently the most diverse lineage of life on Earth with more than 400,000 described species. The group also contains unbelievable ecological and morphological variation that has been evolving since the early Carboniferous (>350 MYA). The group provides the perfect playground to test evolutionary hypotheses with large-scale phylogenies. First, a newly developed bioinformatics tool is presented that allows for the critical assessment of the stability of phylogenetic nodes with even minor changes in taxon sampling (i.e. a single taxon jackknifed). The tool, TANOS, is capable of performing tests on large genomics-scale datasets. The case study included is a …

Computationally Modeling Dynamic Biological Systems, Katherine Jarvis

Electronic Theses and Dissertations

Modeling biological systems furthers our understanding of dynamic relationships and helps us make predictions of the unknown properties of the system. The simple interplay between individual species in a dynamic environment over time can be modeled by equation-based modeling or agent- based modeling (ABM). Equation based modeling describes the change in species quantity using ordinary differential equations (ODE) and is dependent on the quantity of other species in the system as well as a predetermined rates of change. Unfortunately, this method of modeling does not model each individual agent in each species over time so individual dynamics are assumed to …

Predation Efficiency Of Lionfish (Pterois Volitans And P. Miles) In Differing Levels Of Habitat Complexity, Abigail Ehlers Kimbrel

All HCAS Student Capstones, Theses, and Dissertations

The invasion of the Indo-Pacific lionfish (Pterois sp.) into the Atlantic and Caribbean has received attention around the globe. Venomous spines, over-sized fanlike pectoral fins, slow movement, and novel feeding strategies have allowed lionfish to become effective apex- predators. Recent research has determined that lionfish use inshore mangrove habitats as foraging grounds, which are also used as nursery habitats for juvenile fish, thus reducing recruitment of native fish populations. The purpose of this study was three-fold: (1) determine the predation efficiency of lionfish in differing mangrove habitat complexities, (2) examine the influence of temperature and behavior of prey commonly found …

An Insider's Guide To Cell Biology, Katie Ludwig

Honors Projects

An Insider's Guide to Cell Biology is a comic book that follows Alanine the amoeba who tells the reader all about how the cell works.

An Investigation Of Chronic Pain As A Much-Neglected Symptom Of Hypermobile Ehlers-Danlos Syndrome, Sydney Collins

Honors Theses

Ehlers Danlos Syndrome is a group of heritable loose connective tissue disorders with 13 distinguished subtypes. The hypermobile type of Ehlers Danlos Syndrome (hEDS) is the most common subtype and is caused by a genetic mutation that leads to defective collagen fibrils. This leads to joint instability and hypermobility, skin elasticity, widespread pain, fatigue, and generalized tissue fragility. Chronic pain is reported to be a symptom in as high as 92% of the hEDS population (Voermans et al., 2010); despite this prevalence, there is a significant lack of research, awareness, and treatment standardization regarding pain in hEDS. This literature review …

Effects Of The Inclusion Of Rice Hull Derived Bio-Oil On Wood Pellet Production, Tyler E. Lowe

Theses and Dissertations

Wood pellet production has become an advancing industry for the sake of reducing greenhouse emissions into the atmosphere especially, in European Union countries. Researchers and industry executives seek new methods and materials to improve the pelletization process. Rice hulls or husks has the potential to aid in wood pelletization as they possess high calorific values. This study focuses on using rice hull derived bio-oil from pyrolysis, which will also decrease ash content, as an additive to aid in the wood pelletization process. Using two groups of rice hull derived bio-oil as an additive in wood pelletization: Group 1 uses heavy …

Seasonal Management Strategies For Tarnished Plant Bug, Lygus Lineolaris (Palisot De Beauvois), In Midsouth Cotton Production Systems, Ryan Taylor Mann

Theses and Dissertations

The tarnished plant bug, Lygus lineolaris (Palisot de Beauvois), is considered the most economically important pest of midsouth cotton. This study was designed to understand the impact of tarnished plant bug populations in the later weeks of flowering cotton and residual effects of novaluron with subsequent applications. Experiments evaluated dynamic threshold approaches in the later flowering period of midsouth cotton, the impacts of novaluron plus subsequent applications at the 3^rd week of square, as well as residual properties in a laboratory experiment. Results suggest that a dynamic late season approach can reduce the amount of insecticide applications targeting tarnished …

Minimizing Off-Target Herbicide Movement Using Novel Application Technology, Hayden B. Quick

Theses and Dissertations

Drift is a point of contention with pesticide applications, causing the need to research application methods that provide consistent efficacy while minimizing off-target movement. Experiments were conducted to evaluate eight undiluted herbicides on invasive woody plants, Chinese tallow (Triadica sebifera) and Callery pear (Pyrus calleryana), when applied individual plant treatment (IPT) via hack-and-squirt. Applications of undiluted aminocyclopyrachlor or imazapyr at 1 ml per 7.6 cm of tree diameter at breast height (DBH) made in the spring provided superior control over other herbicides or application timings. CamelBak® hydration reservoirs were evaluated for storage durability with eight undiluted herbicides. A third study …

Virtual Reality And Postural Control: The Virtual Moving Room Paradigm, Hannah R. Freeman

Theses and Dissertations

Virtual reality (VR) is becoming increasingly important in balance training. However, the influence of VR generated visual perturbation, through the novel virtual moving room paradigm, is unknown. Thirty healthy individuals had their static balance assessed on a BTrackS balance plate under eight different conditions: baseline eyes open and eyes closed with and without VR, unexpected toward and away moving VR perturbation, and expected toward and away moving VR perturbation. Multiple statistical analyses were conducted, and the results revealed significantly higher postural sway variables in the unexpected moving toward trials compared to the other moving room conditions; significantly higher postural sway …

Soybean (Glycine Max) Response To Multiple, Sublethal Exposures Of 2,4-D And Dicamba From Vegetative Through Reproductive Growth, Graham Robert Oakley

Theses and Dissertations

This study was conducted to determine whether soybean productivity is affected by multiple, sublethal herbicide exposures. The effects of dicamba and 2,4-D on soybean (Glycine max) productivity was investigated at 17 site-years. Relative to a single exposure of dicamba at R1, an additional exposure at either V3 or R3 reduced yield up to 23%. Three or more applications did not further decrease yields relative to an R1&R3 exposure. For 2,4-D, a single application to V3, R1, R3, or R5 soybean did not affect grain yield. However, two exposures of 2,4-D occurring from V3 through R3 reduced yield 5 to 7%. …

Dissection Of The Genetic Architecture Of Grain Quality In Rice, Shuai Liu

Theses and Dissertations

Rice is an important human staple food for over half of the world’s population. Amylose content (AC), gelatinization temperature (GT), grain protein content (GPC), percentage grain chalkiness (PGC), and mineral content are important parameters for evaluating rice quality, which attracts customers and breeders. Only limited genes or QTLs (OsAAP6, OsGluA2, OsASN1, Chalk5, OsHMA3, etc.) are reported regulating rice GPC, PGC, and mineral content due to the lack of genetic knowledge and molecular markers. To dissect the genetic architecture of rice grain quality regulation, genome wide association studies (GWAS) were performed using two populations …