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Full-Text Articles in Life Sciences

High Rates Of Polygyny In Tropical Mexico Within The Native Range Of Vespula Squamosa., Alex K. Snyder, Kevin J. Loope Nov 2021

High Rates Of Polygyny In Tropical Mexico Within The Native Range Of Vespula Squamosa., Alex K. Snyder, Kevin J. Loope

Honors College Theses

Polygyny, or the formation of colonies with multiple cooperating queens, has been observed in a variety of social Hymenoptera and likely exists as a convergent evolutionary strategy. Polygyne cooperation has been observed in several Vespula sp. and is correlated with a perennial social strategy. This perennial-polygyne behavior has been observed most commonly within the tropical and subtropical regions of the invasive Vespula pensylvanica and V. germanica, and rarely within their native temperate ranges. This phenomenon has been relatively undocumented within the tropical portions the V. squamosa native range, despite it being observed in their temperate ranges several times. We observed …


Molecular Analysis Of Mchm Toxicity In Zebrafish, Cassandra Pelton Oct 2021

Molecular Analysis Of Mchm Toxicity In Zebrafish, Cassandra Pelton

Honors College Theses

In early 2014 Freedom Industries, located in Charleston, WV, leaked approximately 10,000 gallons of 4-methylcyclohexane methanol into the Elk River. This river serviced approximately 300,000 people as a source of municipal water. Its effects on the people and surrounding wildlife is largely unknown.

Chemicals in surface runoff have the potential to induce aquatic animal trait changes, such as altered movement and feeding, that can ultimately alter predator-prey dynamics in aquatic systems. However, chemicals with high potential to enter aquatic systems, like 4-methylcyclohexane methanol (MCHM) used to clean coal, were tested using only short-term, single-species studies prior to use. Data from …


An International Classification Of Functioning, Disability And Health Perspective On Physical Activity Participation Among Children With Cerebral Palsy, Adrenne T. Newell Oct 2021

An International Classification Of Functioning, Disability And Health Perspective On Physical Activity Participation Among Children With Cerebral Palsy, Adrenne T. Newell

Honors College Theses

Children with cerebral palsy do not commonly meet the minimum recommended physical activity guidelines published by the U.S. Department of Health and Human Services. Children with cerebral palsy face many functional limitations which can, in turn, affect participation and activity; therefore, this study aimed to identify the predictors of participation and activity among children with cerebral palsy. The study examined associations among poverty levels, neighborhood support, accommodating services, and familial financial burdens as factors influencing participation. Data were taken from the National Survey of Children’s Health. A chi-square test for the association was executed along with a logistic regression.


Understanding The Mechanistic Roles Of Integrin Alpha 6 In Tumor Development Using A Humanized Zebrafish (Danio Rerio) Model System, Ashley B. Williams Aug 2021

Understanding The Mechanistic Roles Of Integrin Alpha 6 In Tumor Development Using A Humanized Zebrafish (Danio Rerio) Model System, Ashley B. Williams

Honors College Theses

Present day cancer incidence and mortality rates indicate the need for effective cancer diagnostic tools and targeted cancer therapeutic strategies. Recent studies have focused on the biological pathways of cells and tumor microenvironments to identify putative biomarkers and potential drug targets as diagnostic and therapeutic tools. Human integrins, adhesion receptors, have become the focal points in these studies, specifically Integrin Alpha 6 (ITGA6) which has been implicated in major tumor progression roles: metastasis and angiogenesis. These characteristics make ITGA6 an excellent candidate for potential drug or diagnostic target, however, the mechanism by which ITGA6 facilitates tumor progression remains unclear. Cell …


A Forward Genetic Screen To Identify Human Genes Of Interest And Their Roles In Ovarian Cancer, Susan A. Ihejirika May 2021

A Forward Genetic Screen To Identify Human Genes Of Interest And Their Roles In Ovarian Cancer, Susan A. Ihejirika

Honors College Theses

Drosophila melanogaster, fruit flies, are very important for modeling and studying human diseases. This study identifies human genes of interest and their contributions to epithelial ovarian carcinogenesis and progression as well as the roles orthologs of these genes play in Drosophila melanogaster. This is important because ovarian cancer is the most common cause of death among the gynecological cancers. This identification of genes was carried out using a forward genetic screen employing the widely used GMR-Gal4 driver/UAS-transgene system. The GMR-Gal4 driver is commonly utilized to express transgenes in the developing eye of the fruit fly. Transgenes that are expressed using …


Effects Of Synthetic Estrogen (17Α-Ethinyl Estradiol) On Male Fiddler Crab Aggression, Micayla Shirley May 2021

Effects Of Synthetic Estrogen (17Α-Ethinyl Estradiol) On Male Fiddler Crab Aggression, Micayla Shirley

Honors College Theses

Pharmaceuticals, including hormones and antibiotics, are considered contaminants due to their widespread use and release into the environment. Hormones, like the synthetic estrogen used in oral contraceptives (17α-ethinylestradiol), are present in freshwater and marine systems, but with relatively unknown effects on the organisms that live there. Ethinylestradiol (EE2) accumulates in waterlogged soil (sediment) with potential to harm sediment-dwelling animals. For example, fiddler crabs (Uca pugilator) are vital members of salt marsh communities. Their burrowing adds oxygen to sediments and cycles nutrients, and they are an important food resource to birds and raccoons. Male fiddler crabs are territorial, aggressively …


Characterization Of Cuticular Hydrocarbon Profiles In Southeastern Argentine Ant (Linepithema Humile) Colonies, Katherine R. Barrs Apr 2021

Characterization Of Cuticular Hydrocarbon Profiles In Southeastern Argentine Ant (Linepithema Humile) Colonies, Katherine R. Barrs

Honors College Theses

Argentine ants, Linepithema humile, are a globally invasive species that displaces native species and facilitates agricultural pests. Colonies occupy distinct territories with multiple nests and defend them against other colonies. Ants have a waxy coating of cuticular hydrocarbons (CHCs) and can detect (i.e. smell) differences in these CHCs to identify their own vs foreign colonies. In the US, Argentine ants have been primarily studied in California where they only form four massive colonies showing mutual aggression, some spanning more than 500 miles. In the Southeast, colony recognition remains largely unexplored; however, we have identified several smaller colonies based on …


Heart Health: Lifestyle Factors That Influence Cardiovascular Well-Being, Holly K. Sawyer Apr 2021

Heart Health: Lifestyle Factors That Influence Cardiovascular Well-Being, Holly K. Sawyer

Honors College Theses

Each year, one in every four deaths in the United States is a result of heart disease. As the leading cause of death among women and men from various ethnicities, heart disease may be overlooked when making everyday life decisions. As a group of genetic and behavioral conditions, heart disease is the number one killer of Americans. This may be due to the typical Western diet and daily life choices such as drinking alcohol or smoking tobacco. Heart diseases including atherosclerosis and coronary artery disease kill ten times more women than breast cancer (Lewis, et al., 2017). White men have …


Evolution And Development Of Staminodes In Paronychia (Caryophyllaceae), Andrea D. Appleton Mar 2021

Evolution And Development Of Staminodes In Paronychia (Caryophyllaceae), Andrea D. Appleton

Honors College Theses

Staminodes are infertile stamens that have evolved numerous times in flowering plants and exhibit a vast array of forms and functions. Variation in staminodes suggests that numerous evolutionary processes underlie their origins, but to understand their how and why they evolved, comparative studies are needed in groups of closely related species. Identifying structures as staminodes is not always straightforward and sometimes requires corroborating phylogenetic and developmental evidence. Staminodial structures in Paronychia (Caryophyllaceae), for example, vary in shape and size and have been referred to as both petals and staminodes, rendering their homology uncertain. The development of staminodes was compared across …


The Relationship Between Sleep Duration And Optimism Levels In Generation Z Students, Anna Hassett Mar 2021

The Relationship Between Sleep Duration And Optimism Levels In Generation Z Students, Anna Hassett

Honors College Theses

Generation Z is getting less sleep than ever and in conjunction, the quality of Generation Z’s sleep is decreasing. Inadequate sleep can increase the risk of depression, cardiovascular diseases, obesity, and cognitive decline. Optimism is a subjective measure of worldview. In the field of positive psychology, optimism is believed to improve overall psychological and physical health. The main purpose of the present study is to examine the relationship between sleep duration and reported levels of optimism in Generation Z students, aged 17 to 24. The hypothesis for this study is that optimism and duration of sleep will have a significantly …


Examining The Mechanistic Roles Of Integrin Alpha-6 In Cancer Metastasis., Chase T. Clark Mar 2021

Examining The Mechanistic Roles Of Integrin Alpha-6 In Cancer Metastasis., Chase T. Clark

Honors College Theses

Metastasis- the spread of cancer cells from the primary tumor to the surrounding tissues- is responsible for 90% of cancer deaths. Integrin alpha-6 (ITGA6) is a specific transmembrane cell surface protein that functions in cell surface adhesion and signaling. ITGA6 is upregulated in many types of cancers and promotes the migration and invasion of cancer cells to surrounding tissues. It is my objective to determine the mechanism through which ITGA6 facilitates the migration of cancer cells through the extracellular matrix (ECM). These experiments helped to establish the role of ITGA6 in tumor development and provide focus for possible chemotherapeutic treatment. …


The Influence Of Autism Linked Gene Topoisomerase 3b (Top3b) On Neural Development In Zebrafish, Sydney Doolittle Mar 2021

The Influence Of Autism Linked Gene Topoisomerase 3b (Top3b) On Neural Development In Zebrafish, Sydney Doolittle

Honors College Theses

Autism Spectrum Disorder is a class of developmental disabilities characterized by a spectrum of social, communication, and behavioral impairments in affected individuals. Studies have shown these defects stem from abnormal brain development during critical periods during early development. The underlying genetic cause of these impairments is not well understood but is believed to be a combination of a complex pairing of genetic and environmental factors. One of the genetic factors that has been recognized to influence the phenotypic symptoms of ASD is the enzyme topoisomerase 3β (top3β.) Topoisomerases are responsible for the prevention of supercoiling during DNA replication. Top3β is …


Long-Term Disability Following A Hamstring Injury, Savannah Mclain Jan 2021

Long-Term Disability Following A Hamstring Injury, Savannah Mclain

Electronic Theses and Dissertations

PURPOSE: Injuries to the hamstring complex are one of the most commonly seen lower extremity injuries in athletic populations. It is currently unknown how psychological or sociological factors affect an athlete after the recovery process has ended and if these factors play a role in re-injury rates relating to the hamstring. It is also unknown if athlete confidence level changes while in a fatigued state after an athlete returns to play from a hamstring injury. METHODS: Twenty-six physically active adults with and without a previous hamstring injury were recruited for this study. Participants completed a Qualtrics survey that …


Study Of Student Resource Use In Introductory Chemistry Courses, Sarah E. Melvin Jan 2021

Study Of Student Resource Use In Introductory Chemistry Courses, Sarah E. Melvin

Electronic Theses and Dissertations

According to the U.S. Government Accountability Office (GAO), college textbook prices have increased by 186% from 1986 to 2004.1 The impact of rising cost of textbooks is increasingly apparent as students are becoming more selective in the courses they enroll in, as well as more concerned with the quality of the learning experience they receive once enrolled in a course.1 In response to high textbook prices, open-education resources (OER) are increasingly becoming more accepted for student use as an alternative to traditional textbook options. OERs are open-source textbook and/or materials that are free to use without worrying about copyright …


Lipid Metabolites As Energy Stores In Four Stingray Species, Lauren Moniz Jan 2021

Lipid Metabolites As Energy Stores In Four Stingray Species, Lauren Moniz

Electronic Theses and Dissertations

Assessing macronutrient transfer is important for estimating ecosystem health and structure. This nutrient transfer is facilitated through trophic position interactions and the consumption of biomass. Lipids are macronutrients that can be used to assess energy flow. Triglyceride (TAG) and free fatty acids (FFA) are important lipids that are obtained from diet and integrate into tissues. They are representative of energy stores and potential energy available for metabolic processes. In marine ecosystems, stingrays occupy the mesopredator niche, facilitating nutrient transfer from lower to higher trophic positions. Stingrays consume a variety of prey items ranging in lipid content, but how lipid metabolites …


Transcriptional Regulation Of Duox-Dependent Reactive Oxygen Species Production Against Bacterial Infection In The Gut Of Cat Fleas (Ctenocephalides Felis)., Ryne W. Maness Jan 2021

Transcriptional Regulation Of Duox-Dependent Reactive Oxygen Species Production Against Bacterial Infection In The Gut Of Cat Fleas (Ctenocephalides Felis)., Ryne W. Maness

Electronic Theses and Dissertations

Fleas (Order Siphonaptera) are opportunistic blood feeders that parasitize a wide variety of mammals and birds. They also transmit bacterial pathogens that cause diseases in humans (e.g., murine typhus, flea-borne spotted fever, cat scratch disease, and plague). Because they acquire infectious pathogens while blood feeding, the flea gut is considered to be the initial site of infection. While immune responses have been well documented in other disease vectors, few studies have identified the immune mechanisms involved in defense of the flea gut. In other hematophagous insects, the synthesis of reactive oxygen species (ROS) is the immediate immune defense mechanism against …


Assessing Short-Term Microalgal Community Responses Over Altering Hydrological Conditions In Experimental Wetlands Of Costal Georgia, Maggie M. Kuhn Jan 2021

Assessing Short-Term Microalgal Community Responses Over Altering Hydrological Conditions In Experimental Wetlands Of Costal Georgia, Maggie M. Kuhn

Electronic Theses and Dissertations

Wetlands provide valuable ecosystem services such as nutrient cycling and carbon storage. Microalgae (i.e., diatoms) provide the foundation of primary production and determine rates of energy transfer throughout the system. Climate change models predict an increase in frequency of intense storms and severe drought conditions that pose a threat to known hydrological regimes and wetland ecosystem stability. The purpose of my study was to assess the effects of water permanence (i.e., duration of flooding) on diatom community structure in experimental wetlands. I predicted that wetlands with prolonged periods of flooding (i.e., permanent) would harbor a consistent diatom community throughout flooding …


Creating A Georgia Southern Spider Collection: Can Dna Barcoding Help?, Guy B. Hobbs Jan 2021

Creating A Georgia Southern Spider Collection: Can Dna Barcoding Help?, Guy B. Hobbs

Electronic Theses and Dissertations

With over 280 spider (Araneae) species recorded within the State of Georgia, USA, the need for a well-documented natural history collection with a usable voucher system is critical to continually assess spider diversity and their future ecological impact in this region. Spider identification can be daunting for the inexperienced taxonomist; it is time consuming and sometimes requires destructive procedures. Previous works have successfully used an alternative method, DNA barcoding, to correctly identify spider species while preserving their morphology. This study set forth to create the core of a well-documented spider collection within Georgia Southern University’s Institute for Coastal Plain Science …


The Relationship Between Emotion Regulation And Health Status In Southeast Rural Firefighters, Catherine G. Gallagher Jan 2021

The Relationship Between Emotion Regulation And Health Status In Southeast Rural Firefighters, Catherine G. Gallagher

Electronic Theses and Dissertations

Firefighters are continuously interacting within high-stress and high physical demanding tasks. Minimal research investigates the influence of emotion regulation on health status among firefighters. PURPOSE: To determine the relationship between emotion regulation constructs and health status fitness metrics in a sample of career firefighters from southeast Georgia. METHODS: Forty-four career firefighters from southeast rural Georgia participated in a yearly fitness assessment. Body composition and fitness variables were collected. Emotion regulation was examined using the 36-item Difficulties in Emotion Regulation Survey. Inferential analysis including a Kruskal Wallis test was used to reveal differences between variables in health status (i.e., Perceived Health, …


Novel Quantification And Localization Of Water And Solute Transporters In The Tissues Of The Spiney Dogfish (Squalus Acanthias), Tolulope B. Ojo Jan 2021

Novel Quantification And Localization Of Water And Solute Transporters In The Tissues Of The Spiney Dogfish (Squalus Acanthias), Tolulope B. Ojo

Electronic Theses and Dissertations

The dogfish, Squalus acanthias is a marine cartilaginous elasmobranch found in the North Atlantic and Pacific oceans. Dogfish synthesize and excrete urea as a product of nitrogen metabolism. They also convert ammonia into urea and retain this urea, such that their plasma is isosmotic or slightly hyperosmotic to the surrounding seawater. To facilitate the regulation of body fluid and to maintain ionic concentrations and osmotic pressure, Dogfish use ion and solute transporters (e.g., NCC, UT-1) and aquaporin water channel proteins. Studies have identified some of the aquaporin genes in the elasmobranch genome, but their functions are mostly uncharacterized. Recent transcriptomic …


Measuring The Success Of A Coastal Sand Dune Restoration On Tybee Island: Plant Growth, Sand Accretion, And Implications For Management, Shannon Matzke Jan 2021

Measuring The Success Of A Coastal Sand Dune Restoration On Tybee Island: Plant Growth, Sand Accretion, And Implications For Management, Shannon Matzke

Electronic Theses and Dissertations

Coastal communities are developing rapidly in the face of increased risks of sea level rise and hurricanes stemming from anthropogenic climate change. In the US, erosion is projected to cost $530 million/year in property loss, but beaches and dune systems can minimize these losses. Dunes are vital to coastal protection, particularly when they are colonized by native plant species that stabilize sand with their root systems and accumulate sand by trapping particles with their stems and leaves. Dune construction can be used as a nature-based solution to climate change, but more studies are needed to fully understand the best practices …


Free-Roaming Cat Abundance Across A Habitat Gradient, Rachel E. Bird Jan 2021

Free-Roaming Cat Abundance Across A Habitat Gradient, Rachel E. Bird

Electronic Theses and Dissertations

There are an estimated 172 million owned and feral cats in the United States, and wildlife enthusiasts and cat owners are often at odds over how best to manage free-roaming cats. Management is needed because of the documented impacts of free-ranging cats on wildlife. Targeting these management efforts, however, is hampered by an imperfect understanding of cat distribution in the landscape. My study used game cameras and capture-recapture sampling to estimate abundance of free-roaming cats across a habitat gradient in Bulloch County, Georgia, USA. In all, I detected cats at 51% (25/49) sites with a mean of 2.1 cats per …


Habitat Use Of Wintering Henslow's Sparrows (Centronyx Henslowii) In Power Line Right-Of-Ways, Abigail W. Dwire Jan 2021

Habitat Use Of Wintering Henslow's Sparrows (Centronyx Henslowii) In Power Line Right-Of-Ways, Abigail W. Dwire

Electronic Theses and Dissertations

Habitat loss and degradation are the leading causes of grassland bird declines worldwide. The Henslow’s Sparrow (Centronyx henslowii; hereafter HESP) is a grassland bird species of conservation concern that has historically relied on the herbaceous ground-layer of longleaf pine savannas and similar habitats in the southeastern U.S. for food and shelter in the winter. However, due to human development, alterations of habitat, and fire suppression, only fragments of these habitats remain. Over the last decade, surveys have annually documented HESPs using power line right-of-ways (hereafter, ROWs) at several sites in the coastal plain of Georgia as alternative habitat for …


Sexual Dimorphism Of Thermal Preference In Florida Scrub Lizards (Sceloporus Woodi) And Predicting Response To Climate Change In Two Rare Habitats, Sidney E. Anderson Jan 2021

Sexual Dimorphism Of Thermal Preference In Florida Scrub Lizards (Sceloporus Woodi) And Predicting Response To Climate Change In Two Rare Habitats, Sidney E. Anderson

Electronic Theses and Dissertations

The Florida Scrub Lizard (Sceloporus woodi) is endemic to Florida, where it inhabits fragments of xeric sandhill uplands including endangered long-leaf pine and sand pine scrub habitats. Lizards depend on a predictable range of temperatures to maximize their growth and productivity, and to do so, they shuttle among various thermal micro-environments. Thus, the spatial distribution of temperatures in the habitat is important. Habitats dominated by either high or low extremes of an organism’s preference are energetically costly and dangerous (less optimal), especially to gravid females. This study examines thermal preference of a near-threatened species that also inhabits increasingly rare habitats. …


Evaluating St. Catherines Island's Shoreline, Vegetation Line, And The Locations Of Loggerhead Sea Turtle Nests, Sydney O. Davis Jan 2021

Evaluating St. Catherines Island's Shoreline, Vegetation Line, And The Locations Of Loggerhead Sea Turtle Nests, Sydney O. Davis

Electronic Theses and Dissertations

St. Catherines Island is a highly dynamic barrier island on the Georgia coast that is also federally listed critical nesting habitat for loggerhead sea turtles. Understanding how St. Catherines’ shoreline and vegetation is changing over time is geographically important as a potential template for other barrier islands. Measuring sea turtle nest locations will provide insight into their natural patterns and how they adjust those locations on a changing barrier island. Analyzing Moving Boundaries Using R (AMBUR) is implemented in this research to assess the movement of the vegetation and shorelines from 2005-2017 using the End Point Rate (EPR) and Linear …


Predation Threat In A Variable Landscape: Connecting Predation Risk To Nesting Success For The Seaside Sparrow (Ammospiza Maritima Macgilivraii), Corina D. Newsome Jan 2021

Predation Threat In A Variable Landscape: Connecting Predation Risk To Nesting Success For The Seaside Sparrow (Ammospiza Maritima Macgilivraii), Corina D. Newsome

Electronic Theses and Dissertations

Predation, the leading cause of nest-failure in birds, not only exists as a direct threat to nesting success, but may exacerbate other sources of nest mortality. Birds inhabiting Atlantic coastal marshes, such as Seaside Sparrows (Ammospiza maritima; hereafter SESP), are experiencing significant and rapid changes to their habitat, particularly sea level rise (SLR) and encroaching urbanization, that may affect the relative influence of nest predation on overall productivity. For SESPs, SLR presents an inherent threat to nest success in its potential to increase the frequency of nest flooding. In addition to this direct threat, the ability of SESPs …


Comparative Conservation Genomics Of A Suite Of Imperiled Freshwater Mussels, Scott C. Meyer Jan 2021

Comparative Conservation Genomics Of A Suite Of Imperiled Freshwater Mussels, Scott C. Meyer

Electronic Theses and Dissertations

Maintenance of genetic diversity is a critical component to the management and recovery of imperiled species. By ensuring that a species’ genetic diversity is maintained, issues like inbreeding depression and loss of local adaptation can be prevented. However, the genetic diversity of many species are not well-characterized, and the factors that influence a species’ genetic diversity are often not well understood. In the case of imperiled unionid freshwater mussels, it is important to conduct conservation genetic assessments to aid in their management and preserve genetic diversity. This is the first study to conduct a comparative conservation genomic assessment for eight …


Assessing Habitat Suitability Of Ribbed Mussels (Geukensia Demissa) In Georgia Salt Marshes By Examining Predicted Mussel Densities And Mussel Population Parameters, William K. Annis Jr Jan 2021

Assessing Habitat Suitability Of Ribbed Mussels (Geukensia Demissa) In Georgia Salt Marshes By Examining Predicted Mussel Densities And Mussel Population Parameters, William K. Annis Jr

Electronic Theses and Dissertations

Geukensia demissa (ribbed mussel) is an ecology important bivalve that has the potential to contribute to salt marsh restoration. Understanding the factors that contribute to the distribution of mussels can help inform managers on choosing locations to optimize the survivorship of mussels in restoration projects. This study sought to model mussel densities across the coast of Georgia and to compare predicted mussel densities with mussel population parameters as means to gauge habitat suitability. Mussel densities were collected through field surveys across a range of salt marshes along the coast of Georgia and were compared with spatial data such as distance …


The Role Of Symbiotic Algae In The Acclimatization Of Oculina Arbuscula To Ocean Acidification, Erin M. Arneson Jan 2021

The Role Of Symbiotic Algae In The Acclimatization Of Oculina Arbuscula To Ocean Acidification, Erin M. Arneson

Electronic Theses and Dissertations

Ocean acidification (OA) caused by CO2 emissions is projected to decrease seawater pH to 7.6 by 2100. Scleractinian corals are at risk because excess H+ in seawater binds to carbonate (CO32-), reducing its availability for CaCO3 skeletons. The energy demand for skeletal growth increases as pH decreases because corals must actively purge excess H+ from their seawater sourced calcifying fluid to maintain high calcification rates. In scleractinian corals it is hypothesized that photosynthesis by symbiotic algae is critical to meet this increased energy demand. To test this hypothesis, I conducted laboratory and field …


Assessing The Utility Of Environmental Dna Techniques To Monitor White Shrimp (Litopenaeus Setiferus) Abundance On The Georgia Coast, Raven Hurt Jan 2021

Assessing The Utility Of Environmental Dna Techniques To Monitor White Shrimp (Litopenaeus Setiferus) Abundance On The Georgia Coast, Raven Hurt

Electronic Theses and Dissertations

Environmental DNA (eDNA) surveys have developed over the last decade from once being a novel tool to now acting as an effective technology often used in complement to traditional capture surveys for assessing the distribution of organisms in freshwater and marine environments. However, many uncertainties on how to properly develop, operate, and analyze eDNA based techniques still hinder this technology effectiveness in the field. The white shrimp, Litopenaeus setiferus, is a common, commercially and recreationally important species in the United States, with landings exceeding $254 million in 2020. L. setiferus is also used as a key indicator species to changes …