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Topological Changes In The Functional Brain Networks Induced By Isometric Force Exertions Using A Graph Theoretical Approach: An Eeg-Based Neuroergonomics Study, Lina Ismail May 2021

Topological Changes In The Functional Brain Networks Induced By Isometric Force Exertions Using A Graph Theoretical Approach: An Eeg-Based Neuroergonomics Study, Lina Ismail

Electronic Theses and Dissertations, 2020-

Neuroergonomics, the application of neuroscience to human factors and ergonomics, is an emerging science focusing on the human brain concerning performance at work and in everyday settings. The advent of portable neurophysiological methods, including electroencephalography (EEG), has enabled measurements of real-time brain activity during physical tasks without restricting body movements. However, the EEG signatures of different physical exertion activity levels that involve the musculoskeletal system in everyday settings remain poorly understood. Furthermore, the assessment of functional connectivity among different brain regions during different force exertion levels remains unclear. One approach to investigating the brain connectome is to model the underlying …


Strategies For Successful Mangrove Living Shoreline Stabilizations In Shallow Water Subtropical Estuaries, Rebecca Fillyaw Jan 2021

Strategies For Successful Mangrove Living Shoreline Stabilizations In Shallow Water Subtropical Estuaries, Rebecca Fillyaw

Electronic Theses and Dissertations, 2020-

Mangrove living shorelines are an effective alternative to hard-armoring, which combat erosion while also increasing habitat. To improve the success of future mangrove deployments, an experimental Rhizophora mangle living shoreline was deployed within Mosquito Lagoon, FL. A factorial design was used to test the impact of mangrove age, breakwater presence, and mangrove placement on mangrove survival and growth. Environmental factors were monitored to isolate the reason for mangrove mortalities. Mangrove age was represented by 3 developmental stages: "seedlings" at 11-months-old, "transitionals" at 23-months-old, and "adults" between 35 and 47-months-old. Mixed mangrove age groups were included to identify if seedling survival …


Regulation Of Actin Assembly, Mechanics, And Structure By Intracellular Environmental Factors, Nicholas Castaneda Jan 2021

Regulation Of Actin Assembly, Mechanics, And Structure By Intracellular Environmental Factors, Nicholas Castaneda

Electronic Theses and Dissertations, 2020-

The assembly, mechanics, and structure of actin cytoskeleton are critical for eukaryotic cellular processes such as structural support, cellular motility, and intracellular transport. Actin assembly occurs in crowded intracellular environments containing many ions and macromolecules. While the individual roles of cation and crowding on actin assembly and mechanics are well established, how the combined cation and crowding effects modulate the formation, mechanics and structural transitioning of actin filaments and bundles are not fully understood. Here, we investigate the impacts of various cation and crowded environments on the bending stiffness, length, organization, secondary structure, and kinetics of actin filaments and bundles. …


Evaluating Carbon Uptake And Storage Potential Of Three Pine Species Across Environmental Gradients In Florida, Alicia Formanack Jan 2021

Evaluating Carbon Uptake And Storage Potential Of Three Pine Species Across Environmental Gradients In Florida, Alicia Formanack

Electronic Theses and Dissertations, 2020-

Natural climate solutions, such as reforestation, are increasingly called for to lower atmospheric CO2 concentrations and prevent further warming of the climate. Predictive modeling of forest stand dynamics provides a quantitative framework that can be used to select the tree species with the highest carbon (C) uptake potential for restoration efforts based upon site-specific and species-specific data. To simulate stand dynamics and compare C uptake and storage potential of three pine species across Florida, I developed individual-based models that combined environmentally-sensitive tree growth models with background mortality taken from the literature. Growth model parameters were estimated using space for time …


High Risk Environmental Conditions Attenuate Distance, Speed, And Performance Efficiency Index In Ncaa D1 Female Soccer Players, Maxine Furtado Mesa Jan 2021

High Risk Environmental Conditions Attenuate Distance, Speed, And Performance Efficiency Index In Ncaa D1 Female Soccer Players, Maxine Furtado Mesa

Electronic Theses and Dissertations, 2020-

PURPOSE: To evaluate the effects of environmental conditions on running performance and performance efficiency index (Effindex). METHODS: Performance data recorded using Polar Pro sensors from eight collegiate female soccer players in nine matches were analyzed during the 2019 competitive season. Effindex and running performance, including total distance covered relative to minutes played (TDREL) and distance covered in five-speed thresholds, were examined for indications of fatigue with rising environmental conditions, including ambient temperature and relative humidity. Matches were separated into three groups based on environmental condition risk: low (Low-Risk; n= 2 matches), moderate (Moderate-Risk; n=3 matches), or high (High-Risk; n=4 matches). …


Characterization And Reliability Of The Work-Time Relationship During Arm-Cranking, Tristan Starling-Smith Jan 2021

Characterization And Reliability Of The Work-Time Relationship During Arm-Cranking, Tristan Starling-Smith

Electronic Theses and Dissertations, 2020-

INTRODUCTION: The critical power (CP) exercise test provides 2 measures, CP and anaerobic working capacity (AWC). CP represents a fatigue threshold that separates the heavy and severe exercise domains, while AWC represents the finite amount of work that can be done above CP. A relatively new protocol for estimating CP has emerged and is described as the 3-minute all-out test (3MT). While this lower body 3MT test has been examined for validity and reliability, very little has been done for the upper body. PURPOSE: Examine the reliability of EP and WEP from the 3MT during upper-body ergometry. This study also …


Utility Of Novel Rotational Load-Velocity Profiling Methods In Collegiate Softball Players, Chad Herring Jan 2021

Utility Of Novel Rotational Load-Velocity Profiling Methods In Collegiate Softball Players, Chad Herring

Electronic Theses and Dissertations, 2020-

The purposes of this study were to determine the reliability of the bat swing (BS) and rotational medicine ball throw (RMBT) load-velocity profiling (LVP) methods and the relationships between LVP variables and batting performance in NCAA Division I softball players. Current NCAA Division I softball athletes participated in this study. Bat velocity was tracked with a swing sensor during the BS method. An inertial measurement unit (IMU) tracked forearm velocity during the BS and RMBT methods. Two-way intraclass correlation coefficients (ICC) were used for relative reliability and coefficient of variation (CV) was used for absolute reliability. For the BS method …


Evaluating The Nutritional Adequacy Of Peruvian-American Children, Delainey Dietz Jan 2021

Evaluating The Nutritional Adequacy Of Peruvian-American Children, Delainey Dietz

Honors Undergraduate Theses

As people move and integrate cultures, nutritional trends may change from those of their native country to those of their new country. With today's increasing global immigration, studies have only scratched the surface of the impact of acculturation on children's health and nutrition. Specifically, the Peruvian-American diet is one that has garnered minimal research. The purpose of this study is to evaluate the common foods and drinks that Peruvian-American children consume, assess the children's physical health, and discuss the possible causative factors of any diet trends. The population sampled is children under 18 years living in the United States whose …


The Effect Of Aerobic Exercise On Children With Autism Spectrum Disorder: A Systematic Literature Review, Delaney K. Cole Jan 2021

The Effect Of Aerobic Exercise On Children With Autism Spectrum Disorder: A Systematic Literature Review, Delaney K. Cole

Honors Undergraduate Theses

The purpose of this study was to examine the effects that aerobic exercise activities can have on various stereotypical behaviors found in children on the autism spectrum. Autism spectrum disorder (ASD) is a lifelong, developmental disability that can impact how an individual communicates, behaves, as well as moves. Physical activity has long been implemented in the treatment plans of children with diagnoses along the autism spectrum; However, specific data relating to the impact of aerobic activity is quite scattered. Aerobic activities require the body to be able to take oxygen from the air around it and circulate it through the …


The Psychological Benefits Of A Remote Physical Activity Intervention In Young Adults With Autism Spectrum Disorder, Madisyn T. Pelchat Jan 2021

The Psychological Benefits Of A Remote Physical Activity Intervention In Young Adults With Autism Spectrum Disorder, Madisyn T. Pelchat

Honors Undergraduate Theses

Previous studies found that individuals with Autism Spectrum Disorder (ASD) tend to demonstrate poor health behaviors, including unhealthy nutrition habits and low physical activity (PA) levels. Growing research suggests the PA can improve ASD-related symptoms and attenuate the mental and physical adverse effects of the COVID-19 pandemic. Unfortunately, the stay-at-home order was put in place because of COVID-19, which negatively affected health behaviors in many individuals with ASD, including an increase in sedentary behavior and screen time and less PA participation. Prior research also has found that young adults with ASD are at a higher risk for obesity than their …


Developing A Self-Sanitizing Mask To Combat The Spread Of Infectious Disease, Matthew Crawford Jan 2021

Developing A Self-Sanitizing Mask To Combat The Spread Of Infectious Disease, Matthew Crawford

Honors Undergraduate Theses

Masks have become an important part of everyday life, protecting both the wearer and individuals nearby from the spread of infectious diseases, most notably severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the coronavirus that causes coronavirus disease 2019 (COVID-19). However, these masks are easily contaminated, whether through continued use or by the wearer touching the mask fabric with contaminated hands, therefore reducing the efficacy and exposing the user to these contagions. When the mask becomes contaminated, it can be discarded, which produces large amounts of waste that will end up in a landfill, or it can be washed, which is …


The Role Of Ms-818 In Altering Age-Related Characteristics Of An In Vitro Model Of Senescence In Neural Stem Cells, Sandeep Sreerama Jan 2021

The Role Of Ms-818 In Altering Age-Related Characteristics Of An In Vitro Model Of Senescence In Neural Stem Cells, Sandeep Sreerama

Honors Undergraduate Theses

Aging of the brain is the leading risk factor for neurodegenerative diseases and brain cancers and has deleterious effects on brain functions. It follows that attempts to reverse the aging process may be therapeutically valuable. Neural stem cells (NSC) have been shown to play a critical role in maintaining brain functions, and their number is severely decreased with age. The development of senescence-like characteristics and declining functions in NSCs have been proposed to be responsible for brain aging and tumorigenesis. MS-818 is a pyrrolopyrimidine that has been shown to increase the NSC population and reverse the decline of behavioral function …


Phototransduction Components In The Visual System Of Hard-Bodied Ticks, Arimar J. López Limas Jan 2021

Phototransduction Components In The Visual System Of Hard-Bodied Ticks, Arimar J. López Limas

Honors Undergraduate Theses

Ticks are terrestrial invertebrate parasites that attach to their hosts to feed on their blood. Ticks are composed of three families: Ixodidae, Argasidae, and Nuttalliellidae. Ixodid ticks include members of the genera Amblyomma, Ixodes, Haemaphysalis, Hyalomma, Dermacentor, and Rhipicephalus. Ticks can transmit diseases to animals and humans, making them an important organism to study. Current tick-bite mitigation strategies include acaricides (harmful if misused), and CO2 traps (effective in decreasing tick abundance, but costly to use). Since the visual system of ticks has not been studied extensively, I expect that by studying their visual system, alternative tick-bite mitigation …


The Implications Of Case-Based Learning (Cbl) On Biochemistry Education: An Integrative Literature Review, Ryan C. Ripert Jan 2021

The Implications Of Case-Based Learning (Cbl) On Biochemistry Education: An Integrative Literature Review, Ryan C. Ripert

Honors Undergraduate Theses

The COVID-19 has significantly impacted the world as we know it, especially in higher education institutions. In response to COVID-19, most colleges and universities were forced to close their doors to students and switch to online learning over Zoom. Although most students are adjusted well to this new mode of education, other students, such as individuals pursuing degrees in the sciences, have difficulty adapting to the online mode of instruction. Case-based learning (CBL) is considered one of the best strategies in teaching a course like Biochemistry in a large classroom setting. Implementing CBL in biochemistry education may increase student engagement …


Paternal Ages And Genetic Diseases And Congenital Anomalies, Neda Hamood Jan 2021

Paternal Ages And Genetic Diseases And Congenital Anomalies, Neda Hamood

Honors Undergraduate Theses

The purpose of this thesis is to investigate the link between advanced paternal ages (APA) (i.e., APA ≥ 35 years and APA ≥ 50 years) and genetic diseases and congenital anomalies. Currently, the relationship between both advanced paternal ages and genetic diseases and congenital anomalies remains unclear. However, there is room for improvement to systematically investigate the relationship between specific congenital anomalies in newborns and advanced paternal ages. More recently, the link between advanced paternal age (as opposed to existing studies analyzing advanced maternal age alone) and genetic diseases has been recognized by researchers, epidemiologists, and various health experts. Thus, …


Characterization Of A Label-Free Fluorescent Assay For Point Mutation Discrimination Based On Split Aptamer Probes, Shannon A. Beaton Jan 2021

Characterization Of A Label-Free Fluorescent Assay For Point Mutation Discrimination Based On Split Aptamer Probes, Shannon A. Beaton

Honors Undergraduate Theses

Due to the misuse of antibiotics, multi-drug resistant (MDR) bacteria have become more rampant in our society; these MDR have given rise to diseases that are not readily curable. One such agent is the Mycobacterium tuberculosis complex, which is a causative agent of tuberculosis (TB). Timely diagnostics of the bacterial infection and detection of bacterial drug-susceptibility profiles helps to initiate the necessary treatment in a timely fashion and to limit transmission of the disease. For more affordable detection of bacterial diseases, such as TN, tag-free split aptamer probes are advantageous. This proposal aims at designing split aptamer probes for detection …


Invasive Bullfrogs Maintain High Levels Of Immune Gene Diversity Despite Elevated Bd Infection Relative To Native Populations, Jacob Lafond Jan 2021

Invasive Bullfrogs Maintain High Levels Of Immune Gene Diversity Despite Elevated Bd Infection Relative To Native Populations, Jacob Lafond

Electronic Theses and Dissertations, 2020-

Maintenance of genetic diversity at ecologically-relevant loci may be important for allowing invasive populations to become established despite decreases in genomic diversity due to founder effects. To evaluate this prediction, we compared genetic diversity at an expressed MHC class IIß gene fragment to a 909 bp region of the neutral cytochrome b (cytb) locus from 20 populations of the American bullfrog (Rana catesbeiana) across its North American invasive and native ranges and quantified the presence of Batrachochytrium dendrobatidis (Bd), a pathogen, for which R. catesbeiana is a vector species. We recovered 28 unique MHC alleles and found that invasive populations …


A Breathing Intervention To Enhance Cardiac Regulation And Mitigate Stress In Police Cadets, Samantha Napier Jan 2021

A Breathing Intervention To Enhance Cardiac Regulation And Mitigate Stress In Police Cadets, Samantha Napier

Electronic Theses and Dissertations, 2020-

Maintaining effective performance under stress can be challenging, especially in the dangerous environments encountered by the police and military personnel. This document reviews the impact of stress on performance, discusses breath interventions as a means of stress mitigation, suggests an approach for exploring the value of a breath intervention in police cadets, tests, analyzes, and discusses a test of this method and results. Biofeedback training can be used to produce resonance breathing that is synchronized with heart rate and optimizes heart rate variability (HRV). This intervention was expected to alleviate physiological and subjective stress responses. Studies reviewed confirm that higher …


Transcriptomic Analyses Reveal Distinct Responses Of Differentiated And Poorly-Differentiated Intestinal Epithelial Cells To Lipid Peroxides, Nisreen Faizo Jan 2021

Transcriptomic Analyses Reveal Distinct Responses Of Differentiated And Poorly-Differentiated Intestinal Epithelial Cells To Lipid Peroxides, Nisreen Faizo

Electronic Theses and Dissertations, 2020-

Lipid peroxides (LOOHs) abound in processed food and have been implicated in the pathology of diverse diseases including gut, cardiovascular, and cancer diseases. However, the molecular mechanisms by which LOOHs contribute to disease have not been fully characterized. Caco-2 cells have been widely used to model human intestinal epithelium in metabolic studies. As differentiated (Diff) and poorly-differentiated (PDiff) Caco-2 cells represent good models of human enterocytes and intestinal tumor cells, respectively, we investigated the cellular response of Diff and PDiff Caco-2 cells to the most common dietary LOOH, 13-hydroperoxyoctadecadienoic acid (13-HPODE), in terms of differential gene expression, gene ontology and …


Modulation Of Fat Taste By Diet And Hormones, Ashley Calder Jan 2021

Modulation Of Fat Taste By Diet And Hormones, Ashley Calder

Electronic Theses and Dissertations, 2020-

The prevalence of obesity worldwide continues to rise despite efforts to reverse the trend. While many factors contribute to the onset and maintenance of obesity, caloric intake and dietary composition have been shown be primary contributors. The oral cavity is one of the first systems to encounter food and determine its hedonic value. As the gateway to ingestion, the taste system plays a unique role in the initial decisions surrounding the control of food intake. Nutrients like carbohydrates, protein, minerals, and fat all have dedicated systems to allow their recognition at this outermost site of the enteric nervous system. Recent …


Assessing The Role Of Disturbance In Estuarine Fish Community Dynamics, Dakota Lewis Jan 2021

Assessing The Role Of Disturbance In Estuarine Fish Community Dynamics, Dakota Lewis

Electronic Theses and Dissertations, 2020-

Ecological disturbances alter biotic communities and ecosystems. In many coastal zones disturbances are increasing, including algal blooms, storms, hypoxic events, and fish kills. These disturbances are often related, for example blooms releasing toxins or depleting dissolved oxygen, ultimately killing fish. Depending on the intensity, duration, and geographic extent of a disturbance, the fish community can take days to years to recover from disturbances. To explore the relationships among environmental disturbances, sport fish, and forage fish communities, this thesis examined two Florida estuaries with differing disturbance regimes. Using an ensemble modelling approach combining generalized linear models (GLM), Bayesian modelling, and Bayesian …


Monitoring Landscape And Spectral Dynamics Of Subtropical Freshwater Wetlands That Have Undergone Hydrological Restoration, Sarah Parker Jan 2021

Monitoring Landscape And Spectral Dynamics Of Subtropical Freshwater Wetlands That Have Undergone Hydrological Restoration, Sarah Parker

Electronic Theses and Dissertations, 2020-

Over the last century, millions of hectares of wetlands have been lost due to urban development and agricultural activities throughout the world. In the U.S., efforts have been made by federal and state legislation to restore wetland habitat in exchange for development on wetlands. To restore ecosystem function by reestablishing hydrological metrics (e.g., groundwater level fluctuations), wetland restoration aims to facilitate the growth of wetland vegetation to approximate the original conditions as a proxy for ecological integrity. In 1992, the first landscape-scale off-site mitigation project, the Disney Wilderness Preserve (DWP) was funded by the Walt Disney Company in Poinciana, Florida. …


Neuroglobin And Its Role In The Recovery Of Neuronal Cells In Hypoxic Conditions Using Hypoxia Inducible Factor– 1, Riya Shah Jan 2021

Neuroglobin And Its Role In The Recovery Of Neuronal Cells In Hypoxic Conditions Using Hypoxia Inducible Factor– 1, Riya Shah

Honors Undergraduate Theses

Stroke is the world's leading cause of adult disability, caused by lack of oxygen and nutrients to the brain due to a blood clot in a major artery. This leads to ischemic damage of neuronal cells that leads to paralysis, motor, and speech deficits. While most stroke therapies aim at removing or reducing the blood clots in the brain, few treatments target cell damage. Neuroglobin (NGB) is a protein in the brain that is able to aid in neuroprotection following oxidative stress. Hypoxia-Inducible Factor-1 (HIF-1) is a transcription factor that serves as a marker for cell recovery after hypoxia or …


The Relationship Between Rapid Weight Loss And Physical Performance In Combat Sports, Sean P. Cavey Jan 2021

The Relationship Between Rapid Weight Loss And Physical Performance In Combat Sports, Sean P. Cavey

Honors Undergraduate Theses

The purpose of this study is to determine if there is a relationship between rapid weight loss and physical performance in combat sports athletes. The inclusion criteria were rapid weight loss and physical performance effects among combat sports researched in the last 20 years. Phrases, as well as keywords, were searched using multiple databases. The keywords included mixed martial arts (MMA), judo, wrestling, taekwondo, boxing, studies that looked at multiple disciplines at once, and unspecified disciplines. Results of the literature review indicate that there are negative effects on performance due to rapid weight loss and dehydration in combat sports. Appendix …


Linking Magnetoreception To Cacna1d Homolog Protein Insertions In Vertebrates, Annabelle S. Levin Jan 2021

Linking Magnetoreception To Cacna1d Homolog Protein Insertions In Vertebrates, Annabelle S. Levin

Honors Undergraduate Theses

It is well-established that numerous animals can perceive the geomagnetic field. This ability, called magnetoreception, has been studied extensively but the mechanisms for how animals perceive the geomagnetic field remain unclear. Understanding magnetoreception is vital for characterizing the navigational abilities of animals and how biological systems interact with magnetic fields. Recent research examining pigeons, an animal is known to exhibit a magnetic sense, identified two interesting genes that may be involved in magnetoreception. One of these genes, calcium voltage-gated channel subunit alpha1 D (CACNA1D), contains a short amino acid insertion (designated by the amino acids -KKER) in animals known to …


Association Between Plasma Genistein And Health-Related Quality Of Life In Breast Cancer Survivors, Tran Pham Jan 2021

Association Between Plasma Genistein And Health-Related Quality Of Life In Breast Cancer Survivors, Tran Pham

Honors Undergraduate Theses

According to the American Cancer Society, breast cancer is the most commonly diagnosed cancer and is the second leading cause of cancer death in American women. Breast cancer screenings and improvement in treatments have resulted in the rising number of survivors in the recent decade. This urged the need for post-diagnosis lifestyle changes to improve breast cancer patients' quality of life. Many studies found soy food, the primary dietary source of phytoestrogens, has a protective effect against breast cancer recurrence and mortality. Dietary phytoestrogens can be classified into two groups: isoflavones and lignans. Daidzein and genistein were identified as the …


Sequence-Independent Assay For Hiv Viral Load Quantitation, Omar El Merhebi Jan 2021

Sequence-Independent Assay For Hiv Viral Load Quantitation, Omar El Merhebi

Honors Undergraduate Theses

Although nucleic acid tests (NATs) for human immunodeficiency virus (HIV) exhibit many advantages, such as early detection and viral load quantification, over immunological assays, their widespread use is limited by their demand for high-level infrastructure, sophisticated equipment, and advanced staff competence. Furthermore, when quantifying viral loads of patients, it has been reported that these assays can underestimate viral quantities by 22- to 100-fold due to primer-template mismatches in more divergent HIV subtypes. Therefore, we have developed a cost-effective and sequence-independent assay for the detection and quantification of HIV utilizing a modified nucleic acid sequence-based amplification (NASBA) protocol coupled to an …


Consequences Of Stress-Induced Trait Plasticity In Cultivated Helianthus, Gillian Gomer Jan 2021

Consequences Of Stress-Induced Trait Plasticity In Cultivated Helianthus, Gillian Gomer

Honors Undergraduate Theses

The Leaf Economics Spectrum (LES) describes the continuous range of leaf ecophysiological strategies that plants across the globe utilize to achieve a net-positive return on their resource investment. This spectrum is measured by traits such as leaf mass per area, leaf nitrogen, and leaf lifespan. Unsuitable leaf trait combinations are selected against in nature due to a net-loss on the return of initial resource investment, while some potentially extremely effective trait combinations may not be possible due to genetic constraints. Existing genetic variation in LES traits, however, suggests the spectrum may be less rigid than previously expected. To test this, …


Correlations In Microplastic Abundance Between Water, The Eastern Oyster, Crassostrea Virginica, And Their Biodeposits In A Dynamic Florida Estuary, Casey Craig Jan 2021

Correlations In Microplastic Abundance Between Water, The Eastern Oyster, Crassostrea Virginica, And Their Biodeposits In A Dynamic Florida Estuary, Casey Craig

Electronic Theses and Dissertations, 2020-

Estuaries have been identified as hotspots of microplastic pollution because they are transitional zones where coastal freshwater and oceans converge. Microplastics (MP) are transported through estuaries by a dynamic series of forces such as surface flow and tides, which influence MP abundances and trends. The eastern oyster, Crassostrea virginica, is an estuarine bivalve known to ingest MP, resulting in negative impacts on organism physiology. I investigated MP pollution as a threat to C. virginica in a dynamic Florida estuary, the Indian River Lagoon (IRL), and determined there are both regional and small-scale spatial and temporal fluctuations in MP abundance. Tributaries …


Computational Study Of Target Gene Interactions - Enhancers And Micrornas, Amlan Talukder Jan 2021

Computational Study Of Target Gene Interactions - Enhancers And Micrornas, Amlan Talukder

Electronic Theses and Dissertations, 2020-

Gene expression is an essential mechanism for physical and mental development of human. Aberrant regulation of gene expression creates abnormality in human body than can lead to complicated diseases. Gene expression can be regulated at any stage from the chromatin unfolding stage to post-translation stage of protein. In this study, we focused on two important factors of gene expression regulation that participate in the gene expression process at the transcription and the post-transcriptional stages; enhancer-promoter interactions and miRNA-mRNA interactions. The enhancer-promoter interactions are difficult to detect due to the large distance between the enhancer and promoter region and cell-specific activity …