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Articles 1 - 28 of 28
Full-Text Articles in Life Sciences
Topological Changes In The Functional Brain Networks Induced By Isometric Force Exertions Using A Graph Theoretical Approach: An Eeg-Based Neuroergonomics Study, Lina Ismail
Electronic Theses and Dissertations, 2020-
Neuroergonomics, the application of neuroscience to human factors and ergonomics, is an emerging science focusing on the human brain concerning performance at work and in everyday settings. The advent of portable neurophysiological methods, including electroencephalography (EEG), has enabled measurements of real-time brain activity during physical tasks without restricting body movements. However, the EEG signatures of different physical exertion activity levels that involve the musculoskeletal system in everyday settings remain poorly understood. Furthermore, the assessment of functional connectivity among different brain regions during different force exertion levels remains unclear. One approach to investigating the brain connectome is to model the underlying …
Strategies For Successful Mangrove Living Shoreline Stabilizations In Shallow Water Subtropical Estuaries, Rebecca Fillyaw
Strategies For Successful Mangrove Living Shoreline Stabilizations In Shallow Water Subtropical Estuaries, Rebecca Fillyaw
Electronic Theses and Dissertations, 2020-
Mangrove living shorelines are an effective alternative to hard-armoring, which combat erosion while also increasing habitat. To improve the success of future mangrove deployments, an experimental Rhizophora mangle living shoreline was deployed within Mosquito Lagoon, FL. A factorial design was used to test the impact of mangrove age, breakwater presence, and mangrove placement on mangrove survival and growth. Environmental factors were monitored to isolate the reason for mangrove mortalities. Mangrove age was represented by 3 developmental stages: "seedlings" at 11-months-old, "transitionals" at 23-months-old, and "adults" between 35 and 47-months-old. Mixed mangrove age groups were included to identify if seedling survival …
Regulation Of Actin Assembly, Mechanics, And Structure By Intracellular Environmental Factors, Nicholas Castaneda
Regulation Of Actin Assembly, Mechanics, And Structure By Intracellular Environmental Factors, Nicholas Castaneda
Electronic Theses and Dissertations, 2020-
The assembly, mechanics, and structure of actin cytoskeleton are critical for eukaryotic cellular processes such as structural support, cellular motility, and intracellular transport. Actin assembly occurs in crowded intracellular environments containing many ions and macromolecules. While the individual roles of cation and crowding on actin assembly and mechanics are well established, how the combined cation and crowding effects modulate the formation, mechanics and structural transitioning of actin filaments and bundles are not fully understood. Here, we investigate the impacts of various cation and crowded environments on the bending stiffness, length, organization, secondary structure, and kinetics of actin filaments and bundles. …
Evaluating Carbon Uptake And Storage Potential Of Three Pine Species Across Environmental Gradients In Florida, Alicia Formanack
Evaluating Carbon Uptake And Storage Potential Of Three Pine Species Across Environmental Gradients In Florida, Alicia Formanack
Electronic Theses and Dissertations, 2020-
Natural climate solutions, such as reforestation, are increasingly called for to lower atmospheric CO2 concentrations and prevent further warming of the climate. Predictive modeling of forest stand dynamics provides a quantitative framework that can be used to select the tree species with the highest carbon (C) uptake potential for restoration efforts based upon site-specific and species-specific data. To simulate stand dynamics and compare C uptake and storage potential of three pine species across Florida, I developed individual-based models that combined environmentally-sensitive tree growth models with background mortality taken from the literature. Growth model parameters were estimated using space for time …
High Risk Environmental Conditions Attenuate Distance, Speed, And Performance Efficiency Index In Ncaa D1 Female Soccer Players, Maxine Furtado Mesa
High Risk Environmental Conditions Attenuate Distance, Speed, And Performance Efficiency Index In Ncaa D1 Female Soccer Players, Maxine Furtado Mesa
Electronic Theses and Dissertations, 2020-
PURPOSE: To evaluate the effects of environmental conditions on running performance and performance efficiency index (Effindex). METHODS: Performance data recorded using Polar Pro sensors from eight collegiate female soccer players in nine matches were analyzed during the 2019 competitive season. Effindex and running performance, including total distance covered relative to minutes played (TDREL) and distance covered in five-speed thresholds, were examined for indications of fatigue with rising environmental conditions, including ambient temperature and relative humidity. Matches were separated into three groups based on environmental condition risk: low (Low-Risk; n= 2 matches), moderate (Moderate-Risk; n=3 matches), or high (High-Risk; n=4 matches). …
Characterization And Reliability Of The Work-Time Relationship During Arm-Cranking, Tristan Starling-Smith
Characterization And Reliability Of The Work-Time Relationship During Arm-Cranking, Tristan Starling-Smith
Electronic Theses and Dissertations, 2020-
INTRODUCTION: The critical power (CP) exercise test provides 2 measures, CP and anaerobic working capacity (AWC). CP represents a fatigue threshold that separates the heavy and severe exercise domains, while AWC represents the finite amount of work that can be done above CP. A relatively new protocol for estimating CP has emerged and is described as the 3-minute all-out test (3MT). While this lower body 3MT test has been examined for validity and reliability, very little has been done for the upper body. PURPOSE: Examine the reliability of EP and WEP from the 3MT during upper-body ergometry. This study also …
Utility Of Novel Rotational Load-Velocity Profiling Methods In Collegiate Softball Players, Chad Herring
Utility Of Novel Rotational Load-Velocity Profiling Methods In Collegiate Softball Players, Chad Herring
Electronic Theses and Dissertations, 2020-
The purposes of this study were to determine the reliability of the bat swing (BS) and rotational medicine ball throw (RMBT) load-velocity profiling (LVP) methods and the relationships between LVP variables and batting performance in NCAA Division I softball players. Current NCAA Division I softball athletes participated in this study. Bat velocity was tracked with a swing sensor during the BS method. An inertial measurement unit (IMU) tracked forearm velocity during the BS and RMBT methods. Two-way intraclass correlation coefficients (ICC) were used for relative reliability and coefficient of variation (CV) was used for absolute reliability. For the BS method …
Invasive Bullfrogs Maintain High Levels Of Immune Gene Diversity Despite Elevated Bd Infection Relative To Native Populations, Jacob Lafond
Invasive Bullfrogs Maintain High Levels Of Immune Gene Diversity Despite Elevated Bd Infection Relative To Native Populations, Jacob Lafond
Electronic Theses and Dissertations, 2020-
Maintenance of genetic diversity at ecologically-relevant loci may be important for allowing invasive populations to become established despite decreases in genomic diversity due to founder effects. To evaluate this prediction, we compared genetic diversity at an expressed MHC class IIß gene fragment to a 909 bp region of the neutral cytochrome b (cytb) locus from 20 populations of the American bullfrog (Rana catesbeiana) across its North American invasive and native ranges and quantified the presence of Batrachochytrium dendrobatidis (Bd), a pathogen, for which R. catesbeiana is a vector species. We recovered 28 unique MHC alleles and found that invasive populations …
A Breathing Intervention To Enhance Cardiac Regulation And Mitigate Stress In Police Cadets, Samantha Napier
A Breathing Intervention To Enhance Cardiac Regulation And Mitigate Stress In Police Cadets, Samantha Napier
Electronic Theses and Dissertations, 2020-
Maintaining effective performance under stress can be challenging, especially in the dangerous environments encountered by the police and military personnel. This document reviews the impact of stress on performance, discusses breath interventions as a means of stress mitigation, suggests an approach for exploring the value of a breath intervention in police cadets, tests, analyzes, and discusses a test of this method and results. Biofeedback training can be used to produce resonance breathing that is synchronized with heart rate and optimizes heart rate variability (HRV). This intervention was expected to alleviate physiological and subjective stress responses. Studies reviewed confirm that higher …
Transcriptomic Analyses Reveal Distinct Responses Of Differentiated And Poorly-Differentiated Intestinal Epithelial Cells To Lipid Peroxides, Nisreen Faizo
Electronic Theses and Dissertations, 2020-
Lipid peroxides (LOOHs) abound in processed food and have been implicated in the pathology of diverse diseases including gut, cardiovascular, and cancer diseases. However, the molecular mechanisms by which LOOHs contribute to disease have not been fully characterized. Caco-2 cells have been widely used to model human intestinal epithelium in metabolic studies. As differentiated (Diff) and poorly-differentiated (PDiff) Caco-2 cells represent good models of human enterocytes and intestinal tumor cells, respectively, we investigated the cellular response of Diff and PDiff Caco-2 cells to the most common dietary LOOH, 13-hydroperoxyoctadecadienoic acid (13-HPODE), in terms of differential gene expression, gene ontology and …
Modulation Of Fat Taste By Diet And Hormones, Ashley Calder
Modulation Of Fat Taste By Diet And Hormones, Ashley Calder
Electronic Theses and Dissertations, 2020-
The prevalence of obesity worldwide continues to rise despite efforts to reverse the trend. While many factors contribute to the onset and maintenance of obesity, caloric intake and dietary composition have been shown be primary contributors. The oral cavity is one of the first systems to encounter food and determine its hedonic value. As the gateway to ingestion, the taste system plays a unique role in the initial decisions surrounding the control of food intake. Nutrients like carbohydrates, protein, minerals, and fat all have dedicated systems to allow their recognition at this outermost site of the enteric nervous system. Recent …
Assessing The Role Of Disturbance In Estuarine Fish Community Dynamics, Dakota Lewis
Assessing The Role Of Disturbance In Estuarine Fish Community Dynamics, Dakota Lewis
Electronic Theses and Dissertations, 2020-
Ecological disturbances alter biotic communities and ecosystems. In many coastal zones disturbances are increasing, including algal blooms, storms, hypoxic events, and fish kills. These disturbances are often related, for example blooms releasing toxins or depleting dissolved oxygen, ultimately killing fish. Depending on the intensity, duration, and geographic extent of a disturbance, the fish community can take days to years to recover from disturbances. To explore the relationships among environmental disturbances, sport fish, and forage fish communities, this thesis examined two Florida estuaries with differing disturbance regimes. Using an ensemble modelling approach combining generalized linear models (GLM), Bayesian modelling, and Bayesian …
Monitoring Landscape And Spectral Dynamics Of Subtropical Freshwater Wetlands That Have Undergone Hydrological Restoration, Sarah Parker
Monitoring Landscape And Spectral Dynamics Of Subtropical Freshwater Wetlands That Have Undergone Hydrological Restoration, Sarah Parker
Electronic Theses and Dissertations, 2020-
Over the last century, millions of hectares of wetlands have been lost due to urban development and agricultural activities throughout the world. In the U.S., efforts have been made by federal and state legislation to restore wetland habitat in exchange for development on wetlands. To restore ecosystem function by reestablishing hydrological metrics (e.g., groundwater level fluctuations), wetland restoration aims to facilitate the growth of wetland vegetation to approximate the original conditions as a proxy for ecological integrity. In 1992, the first landscape-scale off-site mitigation project, the Disney Wilderness Preserve (DWP) was funded by the Walt Disney Company in Poinciana, Florida. …
Correlations In Microplastic Abundance Between Water, The Eastern Oyster, Crassostrea Virginica, And Their Biodeposits In A Dynamic Florida Estuary, Casey Craig
Electronic Theses and Dissertations, 2020-
Estuaries have been identified as hotspots of microplastic pollution because they are transitional zones where coastal freshwater and oceans converge. Microplastics (MP) are transported through estuaries by a dynamic series of forces such as surface flow and tides, which influence MP abundances and trends. The eastern oyster, Crassostrea virginica, is an estuarine bivalve known to ingest MP, resulting in negative impacts on organism physiology. I investigated MP pollution as a threat to C. virginica in a dynamic Florida estuary, the Indian River Lagoon (IRL), and determined there are both regional and small-scale spatial and temporal fluctuations in MP abundance. Tributaries …
Computational Study Of Target Gene Interactions - Enhancers And Micrornas, Amlan Talukder
Computational Study Of Target Gene Interactions - Enhancers And Micrornas, Amlan Talukder
Electronic Theses and Dissertations, 2020-
Gene expression is an essential mechanism for physical and mental development of human. Aberrant regulation of gene expression creates abnormality in human body than can lead to complicated diseases. Gene expression can be regulated at any stage from the chromatin unfolding stage to post-translation stage of protein. In this study, we focused on two important factors of gene expression regulation that participate in the gene expression process at the transcription and the post-transcriptional stages; enhancer-promoter interactions and miRNA-mRNA interactions. The enhancer-promoter interactions are difficult to detect due to the large distance between the enhancer and promoter region and cell-specific activity …
Competence Transcriptome Changes In Streptococcus Pneumoniae, Smitha Shambhu
Competence Transcriptome Changes In Streptococcus Pneumoniae, Smitha Shambhu
Electronic Theses and Dissertations, 2020-
Streptococcus pneumoniae is a human commensal and the causative agent of pneumococcal disease. Pneumococci are naturally competent – able to uptake exogenous DNA from the environment and incorporate it into their genome through homologous and non-homologous recombination. Recombination has significantly shaped the evolutionary history of S. pneumoniae, as it allows pneumococci to rapidly adapt to interventions such as antibiotic therapy or vaccine introduction. Recombination frequencies vary considerably across pneumococcal populations; yet the underlying mechanisms for these variations are not well understood. Entry and exit into competence, a state in which the cell can uptake DNA, is tightly regulated through transcriptional …
Contextualizing The Acute Responses To Arm Cycling, Nicolas Clark
Contextualizing The Acute Responses To Arm Cycling, Nicolas Clark
Electronic Theses and Dissertations, 2020-
The prescription of aerobic exercise modalities engaging the arm musculature have the potential to extend the reach of physical activity and promote cardiorespiratory fitness in individuals with lower body impairment due to excessive body mass, and among individuals seeking to complement and diversify standard leg training. This study compared the acute cardiopulmonary responses obtained during arm cycling and leg cycling performed at different intensities among lean and average (LA) and overfat and obese individuals (OFO). Participants were 37 young and relatively healthy adults. They were tested for mode-specific peak power output and work rate at ventilatory threshold during two randomized …
Long-Term Changes In Juvenile Green Turtle Abundance And Foraging Ecology In The Indian River Lagoon, Florida, Christopher Long
Long-Term Changes In Juvenile Green Turtle Abundance And Foraging Ecology In The Indian River Lagoon, Florida, Christopher Long
Electronic Theses and Dissertations, 2020-
Marine turtles are distributed in temperate, sub-tropical and tropical waters and beaches worldwide, often in areas heavily impacted by humans. Although there are many threats to marine turtle populations, the growing threats of nutrient pollution and harmful algal blooms are relatively understudied despite their widespread impacts on coastal marine ecosystems that marine turtles depend on. By studying juvenile green turtles (Chelonia mydas) in the Indian River Lagoon, Florida, where nutrient pollution and HABs are a widespread and longstanding issue, I aimed to conduct a case study of how these threats may affect this federally Threatened species. In Chapter 2, I …
The Motuporamines Act Through Rho1 And Rac To Control Actin Dynamics In Drosophila, Corey Seavey
The Motuporamines Act Through Rho1 And Rac To Control Actin Dynamics In Drosophila, Corey Seavey
Electronic Theses and Dissertations, 2020-
Given that most cancer patients die from metastatic disease, there is an urgent need to develop drugs that inhibit the spread of tumors. Studies show that the motuporamines activate the small GTPase RhoA to prevent cancer cell migration, but little is known about the mechanism of action of dihydromotuporamine C (Motu33) and its synthetic derivative Motu-(CH2)-33. In the present study, I investigated the biomolecular processes of these compounds in Drosophila by reducing the gene dose of positive and negative regulators of actin dynamics. Consistent with previous findings, reduced gene dose of Rho1 (the Drosophila RhoA ortholog) attenuates motuporamine activity confirming …
Spermidine Rescued Ptpn2/22 Function In Crispr-Cas9-Edited T-Cells With Ptpn2/22 Snps Linked To Crohn's Disease And Rheumatoid Arthritis, Ameera Shaw
Electronic Theses and Dissertations, 2020-
Inflammatory autoimmune diseases like Crohn's Disease (CD) and Rheumatoid Arthritis (RA) share some of the same single nucleotide polymorphisms (SNPs) in protein tyrosine phosphatase non-receptor types 2 and 22 (PTPN2/22), which contribute to their pathogenesis. In clinical CD and RA samples, PTPN2:rs478582 and PTPN22:rs2476601 were found to exacerbate a number of inflammatory processes associated with CD and RA. To confirm the role of these SNPs in CD and RA pathogenesis, CRISPR-Cas9 was used to induce the SNPs in T-cells. Cells were also treated with the naturally occurring polyamine, spermidine, to restore PTPN2/22 function and reverse the inflammatory effects of the …
Influence Of Body Composition, Velocity Profiles, And Sex-Related Differences On Army Combat Fitness Test Performance, David Boffey
Influence Of Body Composition, Velocity Profiles, And Sex-Related Differences On Army Combat Fitness Test Performance, David Boffey
Electronic Theses and Dissertations, 2020-
The Army Combat Fitness Test (ACFT) will become the United States Army's mandatory physical fitness test in March of 2022. The purpose of this study was to determine the relationship between ACFT performance and both body composition and velocity profiles, and to determine sex differences for these variables. Data was collected in November 2020 (Fall) and March 2021 (Spring) from male (n = 55) and female (n = 17) Army Reserve Officers' Training Corps (ROTC) cadets. Body composition was assessed with a bioelectrical impedance spectroscopy (BIS) device, and cadets completed a squat jump (SJ) force-velocity profile (FVP) and a hex …
A Drosophila P5b-Type Atpase Is Required For Polyamine Transport, David Brown
A Drosophila P5b-Type Atpase Is Required For Polyamine Transport, David Brown
Electronic Theses and Dissertations, 2020-
In eukaryotes P-type ATPases are represented by a superfamily of membrane ion and lipid transporters divided into five subclasses. Substrates for classes 1-4 have been identified, however little is known about the substrate specificity of the P-type 5A and 5B transporters. Here we show that the Drosophila gene anne boleyn encodes a family of six P5B-type ATPases. Using a combination of RNAi, insertional mutagenesis, and CRISPR-Cas9 gene editing we show that some of these isoforms are required for polyamine transport and others are not. In addition, we show that the Drosophila P5A-type ATPase, encoded by CG6230 is not required for …
A Mathematical Model For Predicting Animal Population Persistence On Fragmented Landscapes, Allyson Jones
A Mathematical Model For Predicting Animal Population Persistence On Fragmented Landscapes, Allyson Jones
Electronic Theses and Dissertations, 2020-
The effects of roads, buildings, and cities on animal populations are widespread and, often times, disastrous. These structures fragment animals' homes, inhibiting their ability to obtain essential resources and to reproduce. The question arises then: Under what circumstances can an animal population persist in a fragmented landscape? To attempt to answer this question, we present a spatially explicit reaction-diffusion model with varying growth and diffusion rates that incorporates animal behavior at points where habitats are fragmented for four different habitats. The outcome of extinction or persistence of the animal population is determined by examining the effects of changing parameters on …
Bacterial Association Networks From Healthy And Cancer-Associated Gut Microbiomes, Mark Loftus
Bacterial Association Networks From Healthy And Cancer-Associated Gut Microbiomes, Mark Loftus
Electronic Theses and Dissertations, 2020-
The human gastrointestinal tract is colonized by a diverse community of symbiotic microorganisms, mainly bacteria, that are known to play essential roles in maintaining the health of their human host. Disruption of this bacterial community has been associated with numerous diseases, including Colorectal Cancer (CRC). CRC is one of the leading causes of cancer-related deaths worldwide. As such, focus has been placed on the modulation of the bacterial community within the cancer-associated gut microbiome as the next step in possible CRC treatment and prevention strategies. To use the bacterial community for these purposes, a better understanding of the associations that …
Bird Abundance And Diversity And The Impact Of Oyster Reef Restoration On The Bird Community In Mosquito Lagoon, Florida, Usa, Jessica Copertino
Bird Abundance And Diversity And The Impact Of Oyster Reef Restoration On The Bird Community In Mosquito Lagoon, Florida, Usa, Jessica Copertino
Electronic Theses and Dissertations, 2020-
Birds are often used as indicators for biodiversity and ecosystem health. While birds have been monitored in other parts of the Indian River Lagoon (IRL), there has been little research on birds in Mosquito Lagoon (ML, the northernmost portion of the IRL). This thesis first examines the abundance and diversity of birds in ML by using two years of photographic observations to assess seasonal variations and the use of various habitat features by specific bird taxa. Abundance and species richness were highest in winter, while evenness and Simpson's diversity were highest in summer. Moreover, natural and artificial habitat features were …
The Effects Of A Remote Based Exercise Intervention On Psychosocial Factors In Young Adults With Autism Spectrum Disorder, Juan Mayo
Electronic Theses and Dissertations, 2020-
AIM: The aim for this study was to identify the effects of physical activity (PA) on the psychosocial factors (PA self-efficacy, enjoyment of PA, perceived stress, and depression) amongst young adults with Autism Spectrum Disorder (ASD) through the use of a 12-week remotely prescribed exercise program. METHODSs: This quasi-experimental study consisted of a 12-week remote-based exercise intervention (and a no-exercise control group) for young adults (18 – 35 years) with ASD. Participants were recruited through the Center for Autism and Related Disabilities (CARD), an organization that provides resources to individuals with ASD and their families. The exercise program occurred twice …
Development Of Adjunctive Agents For Difluoromethylornithine Anti-Cancer Therapy, Aiste Dobrovolskaite
Development Of Adjunctive Agents For Difluoromethylornithine Anti-Cancer Therapy, Aiste Dobrovolskaite
Electronic Theses and Dissertations, 2020-
The native polyamines putrescine, spermidine, and spermine are small positively-charged molecules that can interact with negatively-charged macromolecules like DNA, RNA and proteins. These interactions aid in nucleic acid and protein conformational stability, thereby, making polyamines essential building blocks for cells. Polyamines are involved in various cellular functions including gene regulation, protein synthesis, and cell proliferation. Rapidly-proliferating cells, such as cancer cells, utilize high polyamine levels for cell growth. Targeting the polyamine addiction of cancers is a validated anti-cancer strategy. To achieve the maximal reduction of polyamine levels, polyamine blocking therapy (PBT) employs several compounds in combination. First, one can inhibit …
Non-Lethal Predictors Of Organ Level Metal Contaminants In Central Florida Red-Shouldered Hawks, Jennifer Bouchenot
Non-Lethal Predictors Of Organ Level Metal Contaminants In Central Florida Red-Shouldered Hawks, Jennifer Bouchenot
Electronic Theses and Dissertations, 2020-
Elemental contaminants aggregate in the environment due to human activity and can induce detrimental physiological effects within organisms. Birds exhibit exceptional susceptibility to many pollutants and are popular biomonitoring agents throughout the world. Birds of prey are of special interest due to their substantial biomagnification potential. Lethal sampling of internal organs yields accurate contaminant measurements, but recent sampling trends are endeavoring towards non-destructive sampling such as feathers. This methodological shift stems from researchers' preference for less-regulated samples, enhanced animal welfare, and broadening the possible collaborative audience with simpler protocols. Unfortunately, investigations of feathers have shown that their accuracy is capricious …