Life Sciences Commons^™

Linking Plot And Landscape Level Phenology, Alejandra Flores

Open Access Theses & Dissertations

Plant phenology has become popularize since the 1990s to help understand the effects of climate change on vegetation, it is a way to study the patterns of leaf-out, flowering, and senescing of the life-cycle of the plant. The timing from start of season (SOS) to end-of-season (EOS) of plant leaf-out development has important implication on ecosystem productivity and carbon cycling across ecological scales from individual trees to whole regions. The need for plant phenology studies in dryland are highly anticipated, since they make up 40% of the terrestrial landscape and recently have shown the play an important role in the …

Transgenerational Differences Between Two Culturally Distinct Prairie Vole Populations, Richard Joaquin Ortiz

Open Access Theses & Dissertations

Culture can be described as a system of environmental beliefs, values, and social practices within one’s environment. This system is passed on from generation to generation and provides a basis for an individual’s behaviors and cognitive perceptions. Cultural neuroscience is an emerging field that intertwines domains of anthropology, psychology, neuroscience, and genetics to help understand the underlying processes, neural mechanisms and genomic factors that vary across cultures. Similar to humans, the socially monogamous prairie vole (Microtus ochrogaster) display populational differences in prosocial behavior and aggression based upon region-specific cultural upbringing and parental lineage. Prairie voles originating from Kansas (KS) display …

Long-Term Effects Of Adolescent Fluoxetine Exposure On Hippocampal Gene Expression In Male C57bl/6 Mice, Anapaula Themann

Open Access Theses & Dissertations

Mood-related disorders, including depression and anxiety, are prevalent among children and adolescents. This poses a public health challenge, given their adverse impact on these young populations. Treatment with the selective serotonin reuptake inhibitor fluoxetine (FLX) is the first line of pharmacological intervention in pediatric patients suffering from affect-related illnesses. Although the use of this antidepressant has been deemed efficacious in the juvenile population, the enduring neurobiological consequences of adolescent FLX exposure are not well understood. For this reason, we explored for persistent molecular adaptations, in the adult hippocampus, as a function of adolescent FLX pretreatment. To do this, we administered …

Identification Of Prostate Cancer-Associated Genomic Alterations By Analyzing Variant Frequencies, Functional Effects, And Protein Interactions, Bofei Wang

Open Access Theses & Dissertations

With the ever-increasing varieties of sequencing techniques, the volume and scope of genomic data are explosively expanded, offering unparalleled opportunities for researchers to study gene-disease associations, identify biomarkers, and thus develop more effective diagnostic and therapeutic strategies. In this project, I have developed a computational workflow and a new scoring scheme, which combine statistical frequency-based analyses with two well-established functional effect prediction tools FATHMM and PROVEAN, to evaluate nonsynonymous GSVs and identify potential cancer-related protein-coding genes for downstream enrichment and protein-protein interaction (PPI) studies.

This method has been applied to process a collection of 503 whole exome sequencing datasets for …

Examination Of The Association Between C - Reactive Protein(Crp) And Covid-19 Infection Severity And Length Of Hospitalization, Abdulahi Aremu Ayanwale

Open Access Theses & Dissertations

Chronic stress can lead to many systemic complications and low-grade systemic inflammation including increased levels of inflammatory cytokines, such as C-reactive protein (CRP). CRP is a marker of systemic inflammation and is associated with depression and perceived stress. Elevations can result in ineffective immune responses, thereby increasing the risk of complications and mortality from infections. Recent evidence suggests that uncontrolled inflammatory responses associated with COVID 19 are a major determinant of disease severity.  The COVID 19 is transmitted from one person to another through droplets from coughing, sneezing, talking, touching droplets on surfaces and contamination by hand-to-mouth routes. The mechanism …

Utilizing Computational Approaches To Study Tubulin- Tubulin Electrostatic Interactions, Tolulope Ayodeji Ale

Open Access Theses & Dissertations

Microtubules play significant roles in transporting cargos within the cell, as well as the cell cycle. They are essentially the highway for the transportation of cell organelles. Understanding the interactions among microtubule components is crucial for the treatment of neurodegenerative diseases and cancer. In this study, we utilize a series of computational approaches to study the electrostatic interactions at the binding interfaces of interacting tubulin monomers. Our study revealed that the electrostatic attractive interactions in the αβ tubulin dimer is the strongest while the interaction between two αβ tubulin dimers in the longitude direction is the weakest. The result identified …

In Vitro Investigation Of Tumor Selective Piperidones As Therapeutic Agents Against Leukemia Cancer Cells, Lisett Contreras

Open Access Theses & Dissertations

Cancer is a continuous global health issue. It is the second leading cause of death behind heart disease. Disparities across the emergence of cancer and resulting fatalities raise the importance of researching the disease. Treatments are available for certain types of cancers. However, these are typically accompanied by residual problems including side effects and the possibility for relapse. Some treatments attack all cells, leading to unwarranted side effects that make the possibility of living a comfortable life nearly impossible. Other treatments are specific to certain genetic alterations, making them only useful for a small percentage of patients. Not one treatment …

Comprehensive Study Of Human Pathogenic Trypanosomatids: From Chemotherapies To Disease Ecology, Felipe Rodriguez

Open Access Theses & Dissertations

The vector-borne diseases Leishmaniasis and Chagas disease, caused by the kinetoplastid parasites Leishmania spp. and Trypanosoma cruzi respectively, are among the most important parasitic diseases in the group of neglected tropical diseases. Even though the two diseases are endemic to the Americas, they affect millions of people worldwide. Leishmaniasis and Chagas disease cause a great array of symptoms and some of them can be fatal if left undiagnosed and untreated. Current treatment regimens are becoming less effective, vaccines are still not available, and diagnosis needs to be improved. Vector control has been responsible for a decrease of diseases in endemic …

Molecular Mechanism Of Dna Bisintercalator Antibiotic Resistance, Priyanka Rajendra Gade

Open Access Theses & Dissertations

Class II UvrA is an ATP-binding cassette protein and a homolog of class I UvrA, the DNA lesion detection component of the universal prokaryotic nucleotide excision repair system. We show that Ecm16, a class II UvrA protein from Streptomyces lasalocidi, specifically recognizes DNA duplex that contains the DNA bisintercalator antibiotic echinomycin. Heterologous expression of Ecm16 in Escherichia coli renders host cells resistant to echinomycin. Interestingly, Ecm16’s antibiotic resistance activity does not require the DNA repair proteins UvrA/B/C/D, indicating that it operates independently of the cell’s nucleotide excision repair pathway. Unlike UvrA, Ecm16 uses the insertion domain to regulate its ATPase …

Impact Of Xenobiotics On Neuronal Outcomes, Gabriela Henriquez

Open Access Theses & Dissertations

Neurodegenerative diseases are a common affliction of the Central Nervous System. They are caused by age, genetics, and environmental factors. Alzheimer's disease, Parkinson's disease, Huntington's disease, and Amyotrophic Lateral Sclerosis are well-known among the host of neurodegenerative disorders, with Alzheimer's disease and Parkinson's disease being the leading pathologies across this canvas. It is known that the pathogenesis of neurodegenerative diseases is multifactorial due to the involvement of genetic mutations, radical-induced protein damage, abnormal aggregation of misfolded proteins, and the formation of amyloid deposits. The amyloid proteins in neurodegenerative disease include Tau and Amyloid (Aβ) in Alzheimer's disease and α-Synuclein in …

An Investigation Of Upper Extremity Motor Impairments In Children Diagnosed With Autism Spectrum Disorder: Motor Planning Vs Motor Execution, Patrick Anthony Cereceres

Open Access Theses & Dissertations

Autism Spectrum Disorder (ASD) is a neurodevelopmental disorder typically diagnosedwhen a child displays social and communication impairments, repetitive behaviors, and rhythmic movements like hand flapping. This disorder is unique because of the high variability of symptoms displayed between each individual who is diagnosed. Because of this, no neurological or physiological test exists to diagnose a child with ASD. Current diagnostic tests are various forms of interviews or observations which an experienced clinician must administer. While these tests have been instrumental in diagnosing children with ASD, many go undiagnosed, which can be detrimental as they transition into adulthood. Current literature indicates …

Analyzing Genetic Variation Of The Tussock Cottongrass Among Ecotypes Along A Latitudinal Gradient Through Transcriptomics, Carmen Webster

Open Access Theses & Dissertations

The Arctic is seeing some of the most extreme effects of climate change that induce environmental pressures, including warmer temperatures and longer growing seasons. Due to this, taxa may need to adapt or migrate in order to survive. The long-lived tussock cottongrass, Eriophorum vaginatum, is a foundation species in the Arctic, and little is currently known about the genetic constraints that could be playing a role in how this species will respond to the changing climate. Specific gene families that play an important role in signaling genetic pathways related to plant phenology and response to environmental stress are likely to …

In The Search For Novel Treatments For Chagas' Disease Using Cutting-Edge Imaging Technology, Karsten Dieter Amezcua Winter

Open Access Theses & Dissertations

Chagas disease (ChD) is caused by Trypanosoma cruzi (T. cruzi), an intracellular protozoan parasite. ChD has a global mortality of 15,000 annual deaths, and approximately, 8-10 million people are infected. There is growing concern in the United States as autochthonous cases of ChD have been reported in the southern region. The two available treatments are only partially effective and highly toxic. N-aroyl derivatives and α, β-unsaturated ketones have been previously tested against Leishmania, a closely related parasite, demonstrating selective toxicity towards the microorganism. The objective of this study is to evaluate a drug library consisting of 21 α, β-unsaturated ketones …

Landscape Population And Evolutionary Genomics Of Several Closely Related Species Of Mallard-Like Ducks, Joshua Brown

Open Access Theses & Dissertations

Divergence and speciation proceed through three major evolutionary forces (i.e., selection, genetic drift, and gene flow) that are often spatially and temporally heterogeneous across the landscape. Moreover, these forces can have differing but subtle effects within the genomes of diverging taxa, and therefore, disentangling the effects of these evolutionary mechanisms throughout the speciation process can be challenging. Here, I use a recent species radiation, the mallard complex, to investigate how strong, yet varied, evolutionary pressures influence the speciation process. The mallard complex consists of 14 mallard-like waterfowl species around the world that have some of the highest rates of hybridization …

Articulating The Importance Of Broader Relevance In Cures: From Definition To Actualization, Nikki T. Donegan

Open Access Theses & Dissertations

Course-based undergraduate research experiences (CUREs) have emerged as a means to foster student' scientific reasoning, promote positive attitudes and motivations toward research, and increase persistence in the science, technology, engineering, and mathematics (STEM) fields. In contrast to prescriptive laboratories, students enrolled in CUREs employ techniques and skills to collaboratively and iteratively address novel questions of broad relevance to audiences beyond the scope of the course itself. While many of the core components of CUREs are perceived to be relatively straightforward, definitionally-speaking, this latter dimension of broader relevance has, in contrast, remained largely ill-defined. In the series of studies presented herein, …

The Effects Of The Dual-Tasking: Walking While Texting On Slip Recovery Mechanics, Ileana Abril Jarquin

Open Access Theses & Dissertations

Introduction: The number of induced falls has increased exponentially over the last decade. Previous studies have determined that the cognitive demands of texting affect the processing of cognitive and motor tasks.Research question: What effect does the dual-tasking of texting and walking has on slip recovery mechanics? Methods: Three-dimensional kinematic data were collected while 20 participants between the ages of 18 and 30 years three different conditions; 1) baseline, 2) walking + slip perturbation, and 3) texting and walking + slip perturbation. Results: Walking speed, numbers of falls, and recovery time from the slip were not affected by the texting dual-task. …

Pacific Black Duck Ecology And Habitat Assessment In Aunu'u, American Samoa, And Their Relationship To Other Mallard-Like Ducks Of Oceania, Greater Indonesia, And The Philippines, Marissa Kaminski

Open Access Theses & Dissertations

Determining a species’ ecological needs, assessing the quality of their habitat, and determining genetic differentiation and connectivity among populations is essential to their conservation. My dissertation focuses on obtaining such a holistic view for a population of Pacific Black Ducks recently established on the Island of Aunu’u, American Samoa. Specifically, I present the first evaluation of the ecology and habitat of a recently established population of Pacific Black Ducks on the Island of Aunu’u, American Samoa, in Chapter 1, while I assess the genetic connectivity and relationship of this population to other Mallard-like ducks found in Greater Indonesia, Oceania, and …

The Role Of Calcium Ions In Signaling And Regulation Of Efflux Pump Genes In Staphylococcus Aureus, Amy Rebecca Nava

Open Access Theses & Dissertations

Calcium ions (Ca2+) play an important role in eukaryote cell signaling and regulation of physiological functions. Although evidence of a similar role for Ca2+ in prokaryotes has been difficult to demonstrate, there is mounting evidence that Ca2+ acts as a cell regulator in bacteria. The purpose of this study was to investigate Ca2+ signaling and the effect of Ca2+ on gene expression of various multidrug resistant (MDR) efflux pumps and their regulator MgrA in Staphylococcus aureus and clinically isolated MRSA. We hypothesized that the presence of Ca2+ increased gene expression of multidrug resistance pumps, LmrS, NorA, and the regulator, MgrA. …

Statistical Analysis Of Genetic Sequence Variants In Whole Exome Sequencing Data From Patients With Prostate Cancer, Kelvin Ofori-Minta

Open Access Theses & Dissertations

A single variation in the genetic sequence within the DNA of an organism could easily lead to beneficial, detrimental or neutral effects. Most often than not, these effects are detrimental than beneficial. While many biomedical and bioinformatics studies have been conducted to determine the genetic cause of prostate cancer (PrCa) which is still the second leading cause of cancer related death among men in the United States. An appreciable effort in statistical bioinformatics researches has been directed towards this aim. Through statistical analyses of a set of whole exome sequencing data from patients with PrCa obtained via The Cancer Genome …

Restoring Biogeochemical Properties In Drylands And Exploring Functional Roles Of Biological Soil Crust, Kristina Young

Open Access Theses & Dissertations

Degradation in dryland regions is a persistent and accelerating problem. Though the mechanisms that initiate and maintain dryland degradation have been well studied, restoring productivity and function to degraded dryland ecosystems remains difficult. Here, I present three chapters that address gaps in our understanding of dryland functions and our ability to restore them. I begin by examining how dryland restoration research has addressed altered biogeochemical cycling in drylands and how to expand current understandings of dryland biogeochemistry into restoration. I then present two chapters that explore mechanistic and quantitative understandings of the contribution of biocrusts to soil nutrient cycling both …

Molecular And Cellular Characterizations Of Glucosylceramide Transferase (Synthase) In Giardia Lamblia, Vanessa Enriquez

Open Access Theses & Dissertations

Giardiasis, caused by an intestinal protozoan, Giardia lamblia, is a major public health problem worldwide. Giardia infection is spread through the fecal-oral route via contaminated water and food. It is estimated that over 280 million people are infected with Giardia across the globe. Giardia is a non-invasive parasite and consists of a two-stage life cycle: trophozoites and cysts. Ingested cysts undergo excystation in the proximal small intestine, releasing two trophozoites (excyzoites). Upon reaching the distal small intestine and colonizing there, trophozoites transform into cysts by encystation. It has been reported earlier that the process of encystation induced the synThesis of …

A Proposed Mechanism For Stimulant-Induced Behavioral Sensitization Involving The Transporter Vmat, Valeria Garcia

Open Access Theses & Dissertations

Elucidating mechanisms that drive drug craving is crucial to develop pharmacological interventions for addiction. Repeated exposure to psychostimulants produces an enhanced behavioral and neurochemical sensitivity to both the drug and drug-related cues, known as sensitization. We hypothesize that VMAT, the protein responsible for storage of dopamine (DA) into the vesicles, may be functionally altered to redistribute DA between the cytoplasmic pool and the vesicular pool based on demands for drug-mediated release versus neurotransmission in the context of conditioned environmental cues. To examine the role of VMAT, we pretreated rats with a VMAT blocker, tetrabenazine (TBZ), before each daily treatment of …

A Genomic Approach To Assessing The Fkbp52-Regulated Androgen Receptor Cistrome And Transcriptome, Nina Raquelle Ortiz

Open Access Theses & Dissertations

Androgen receptor (AR)-regulated genes contribute to the initiation and progression of prostate cancer. Receptor signaling persists and plays a pivotal role in late-stages of the disease, for which there is currently no treatment. Thus, there is a need to understand the mechanisms regulating AR function as a means to identify drug targets for potential therapeutics. The FKBP52 cochaperone has emerged in recent years as a regulator of AR activity that is functionally linked in the AR signaling pathway. FKBP52 is a known positive regulator of AR and believed to interact with AR at its binding function 3 surface (BF3), a …

The Hybridizing Ions Treatment (Hit) Method Development And Computational Study On Sars-Cov-2 E Protein., Shengjie Sun

Open Access Theses & Dissertations

Fast and accurate calculations of the electrostatic features for highly charged biomolecules such as DNA, RNA, highly charged proteins, are crucial but challenging tasks. Traditional implicit solvent methods calculate the electrostatic features fast, but they are not able to balance the high net charges in the biomolecules effectively. Explicit solvent methods add unbalanced ions to neutralize the highly charged biomolecules in molecular dynamic simulations, which require more expensive computing resources. Here we developed a novel method, the Hybridizing Ions Treatment (HIT) method, which hybridizes the implicit solvent method with the explicit method to realistically calculate the electrostatic potential for highly …

Applying Computational Methods To Study The Interactions Between Sars-Cov-2 And Hace2, Yixin Xie

Open Access Theses & Dissertations

The ongoing outbreak of COVID-19 has been a serious threat to human health worldwide. The virus SARS-CoV-2 initiates its infection to the human body via the interaction of its spike (S) protein with the human Angiotensin-Converting Enzyme 2 (ACE2) of the host cells. Here we implemented multi-scale computational approaches to study the binding mechanisms of human ACE2 and S proteins of both SARS-CoV and SARS-CoV-2. Electrostatic features, including electrostatic potential, electric field lines and electrostatic forces of SARS-CoV and SARS-CoV-2 were calculated and compared in detail. The results demonstrate that SARS-CoV and SARS-CoV-2 S proteins are both attractive to ACE2 …

Determination Of Perceived Calorie Content Of Common Foods, Sarah Leticia Ruiz

Open Access Theses & Dissertations

Currently, dietary weight-loss interventions focus on recommended caloric intake aimed at creating an energy deficit through the use of calorie-based education. This approach assumes individuals can accurately estimate caloric content of foods and beverages, yet little is known about the validity of such assumptions. This study assessed the accuracy of perceptions of calorie content for individual food items and mixed meals and determined its association with education level, nutrition background, body composition, fruit and vegetable intake, and the domains of eating (emotional eating, uncontrolled eating, and cognitive restraint). A cross-sectional survey assessed calorie perceptions using both visual food displays and …

The Effects Of A Combined Multi-Stressor Environment And Fatigue On Pistol Shooting Performance, Owen Frederick Salmon

Open Access Theses & Dissertations

Acute cold and hypoxia exposure are common environmental stressors to military personnel and can influence human performance (marksmanship, exercise capacity, and strength) in a variety of different ways. Despite both cold and hypoxia being potent independent environmental stressors, both of these environmental stressors commonly coexist in nature and are rarely studied together. It is currently unclear how combined exposure to acute cold and hypoxia will influence pistol shooting performance. Therefore, the present study aimed to determine how combined exposure to a multi-stressor environment will influence pistol shooting performance independently, and in combination with environmental exposure and exercise-induced fatigue. Six subjects …

The Association Between Bilateral Broad Jump Performance And The Sprint Profile, Sergio Alfonso Rodriguez

Open Access Theses & Dissertations

Horizontal force application is vital to attain high velocities during sprinting. Similarly, broad jump performance is associated with the application of horizontal force to achieve greater jump distance. The video analysis-based sprint profile provides insight into multiple measures of sprint performance including maximal theoretical horizontal force, maximal theoretical velocity, optimal velocity, and maximal speed, which give an indication of overall sprint performance that is not reliant on spatiotemporal data alone. PURPOSE: To determine the relationship between broad jump distance and power output and the sprint profile of Division I track and field athletes. METHODS: This study was conducted as a …

Suppression Of Inflammation Of Cytokine Following Induced Francisella Tularensis Infection, Nicole Renee Setzu

Open Access Theses & Dissertations

Francisella tularensis is intracellular bacteria which is the causative agent of the disease Tularemia. Highly virulent in both humans and animals, it takes only as few as 10 microorganisms to cause a lethal infection. The bacteria can enter via direct or indirect routes causing the activations of the host innate inflammatory response to ensue. The bacteria invade host dendritic cells and neutrophils but predominately macrophages. This causes a mass inflammatory response resulting in the cytokine storm. Activation of Natural Killer T (NKT) cells has been shown to suppress inflammation in in vivo studies. Development and optimization of an in vitro …

Strike-Induced Chemosensory Searching, Kleptoparasitism, And The Possibility Of Chemical Crypsis In Rattlesnakes, Mark Scott Teshera

Open Access Theses & Dissertations

Predator-prey interactions shape the natural histories of both species as each tries to adapt and counter-adapt to the tactics of the other. This important evolutionary dynamic is crucial for ecosystem formation and stability. Predation is a fundamental aspect of life for most organisms, and many venomous reptiles, particularly ambush-foraging viperids, have evolved a prey-acquisition strategy that is highly effective in immobilizing prey, while minimizing possible counterattacks. Best studied in rattlesnakes, this remarkable behavioral sequence is known as strike-induced chemosensory searching (SICS). Throughout decades of research, the body of SICS literature on venomous reptiles has become fairly robust, particularly in rattlesnakes. …