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Full-Text Articles in Life Sciences

Development Of Value-Added Butter By Incorporating Whey Protein Hydrolysate-Encapsulated Probiotics, Kritika Gaba, Sanjeev Anand, Athira Syamala Apr 2023

Development Of Value-Added Butter By Incorporating Whey Protein Hydrolysate-Encapsulated Probiotics, Kritika Gaba, Sanjeev Anand, Athira Syamala

Dairy Science and Food Science Faculty Publications: 2022 to Present

The probiotic foods market is growing exponentially; however, probiotics’ survivability and interaction with product attributes pose major challenges. A previous study of our lab developed a spray-dried encapsulant utilizing whey protein hydrolysate-maltodextrin and probiotics with high viable counts and enhanced bioactive properties. Viscous products such as butter could be suitable carriers for such encapsulated probiotics. The objective of the current study was to standardize this encapsulant in salted and unsalted butter, followed by storage stability studies at 4 ◦C. Butter was prepared at a lab-scale level, and the encapsulant was added at 0.1% and 1%, followed by physiochemical and microbiological …


Allometry Of Bud Dynamic Pattern And Linkage Between Bud Traits And Ecological Stoichiometry Of Nitraria Tangutorum Under Fertilizer Addition, Qinghe Le, Na Duan, Chenggong Liu, Huiqing Li, Lan Xu Mar 2023

Allometry Of Bud Dynamic Pattern And Linkage Between Bud Traits And Ecological Stoichiometry Of Nitraria Tangutorum Under Fertilizer Addition, Qinghe Le, Na Duan, Chenggong Liu, Huiqing Li, Lan Xu

Natural Resource Management Faculty Publications

Affected by the pressure and constraints of available resources, plant growth and development, as well as plant life history strategies, usually vary with environmental conditions. Plant buds play a crucial role in the life history of woody plants. Nitraria tangutorum is a common dominant woody species in desertified areas of northern China and its growth is critical to the desert ecosystem. Revealing the allometry of N. tangutorum aboveground bud fates and the linkage between bud traits and plant nutrient contents and stoichiometric ratios can be useful in understanding plant adaptation strategy. We applied seven nitrogen and phosphorus fertilizer addition treatments …


College Of Natural Sciences Newsletter, February 2023, College Of Natural Sciences Mar 2023

College Of Natural Sciences Newsletter, February 2023, College Of Natural Sciences

College of Natural Sciences Newsletters and Reports

Volume 4, Issue 1

Page 1 Dean's Message
Page 2 Awards and Recognition
Page 3-4 Nobel Recipient Visits Campus
Page 4 Adopting the Pantry
Page 5 Growing a Recruitment Mindset
Page 6 February Outreach Events
Page 7 Media Coverage of CNS
Page 8 Open PRAIRIE Data
Page 9 54th Geography Convention, and Tom Loveland EROS Geography Scholarship
Page 10 Photos of Dr. Carolyn Bertozzi's Visit


Associations Between Preweaning Calf Feeding Behaviors With Age At First Calving And Lactational Performance Using An Automatic Calf Feeder, Turner H. Swartz, Christina Petersson-Wolfe Mar 2023

Associations Between Preweaning Calf Feeding Behaviors With Age At First Calving And Lactational Performance Using An Automatic Calf Feeder, Turner H. Swartz, Christina Petersson-Wolfe

Dairy Science and Food Science Faculty Publications: 2022 to Present

The objective of this retrospective observational study was to determine whether preweaning calf behaviors and milk replacer (MR) intake from an automatic calf feeder were associated with age at first calving and first-lactation performance. Calves were housed in groups with access to an automatic calf feeder for 7 wk with a maximum milk allowance of 1,800 g/d of MR (12 L/d). Outcomes of interest included age at first calving (n = 137), first-lactation mature-equivalent 305-d (ME305) milk yield (n = 132), and first-lactation ME305 energy-corrected milk (ECM) yield (n = 132). Linear models included the fixed effects of the daily …


Growth, Yield, And Yield Stability Of Canola In The Northern Great Plains Of The United States, Unius Arinaitwe, Sharon Clay, Thandiwe Nleya Mar 2023

Growth, Yield, And Yield Stability Of Canola In The Northern Great Plains Of The United States, Unius Arinaitwe, Sharon Clay, Thandiwe Nleya

Agronomy, Horticulture and Plant Science Faculty Publications

Canola (Brassica napus L.) may diversify wheat-based cropping systems in the Northern Great Plains. However, agronomic adaptability and stability of high- yielding genotypes have not been widely evaluated over the diverse environmental conditions of South Dakota (SD). A 2-year field experiment was conducted in two contrasting environments (Brookings—eastern SD and Pierre—Central SD) to evaluate genotypes (10 in 2019 and 12 in 2020) for days to 50% flower, lodg- ing, pods plant−1 , seed yield, 1000-seed weight, and yield stability. Seed yield for all genotypes in Brookings averaged 1961 and 1740 kg ha−1 , in 2019 and 2020, respectively, whereas at …


College Of Natural Sciences Newsletter, November 2022 - January 2023, College Of Natural Sciences Feb 2023

College Of Natural Sciences Newsletter, November 2022 - January 2023, College Of Natural Sciences

College of Natural Sciences Newsletters and Reports

Volme 3, Issue 7

Page 1 Dean's Message
Page 2 Awards & Recognition
Page 3 Sioux Falls Middle School Visit
Page 4 Bio-Micro Day of Scholars
Page 5 GIS Day at USGS EROS
Page 6 Indigenous People's Festival, & Visiting Jack's Imagination Lab
Page 7 Media Coverage of CNS, & Research Highlights from Geography & Geospatial Sciences
Page 8 Media Coverage of CNS. cont.
Page 9 Open PRAIRIE Data
Page 10 Recent Publications from CNS
Page 11 Recent Publications from CNS. cont.
Page 12 CNS Holiday Snapshots
Page 13 & 14 Fall 2022 Dean's List
Page 12-14 Fall 2022 Outreach …


2d Respiratory Sound Analysis To Detect Lung Abnormalities, Rafia Sharmin Alice, Kc Santosh Feb 2023

2d Respiratory Sound Analysis To Detect Lung Abnormalities, Rafia Sharmin Alice, Kc Santosh

SDSU Data Science Symposium

Abstract. In this paper, we analyze deep visual features from 2D data representation(s) of the respiratory sound to detect evidence of lung abnormalities. The primary motivation behind this is that visual cues are more important in decision-making than raw data (lung sound). Early detection and prompt treatments are essential for any future possible respiratory disorders, and respiratory sound is proven to be one of the biomarkers. In contrast to state-of-the-art approaches, we aim at understanding/analyzing visual features using our Convolutional Neural Networks (CNN) tailored Deep Learning Models, where we consider all possible 2D data such as Spectrogram, Mel-frequency Cepstral Coefficients …


Session 2: The Effect Of Boom Leveling On Spray Dispersion, Travis A. Burgers, Miguel Bustamante, Juan F. Vivanco Feb 2023

Session 2: The Effect Of Boom Leveling On Spray Dispersion, Travis A. Burgers, Miguel Bustamante, Juan F. Vivanco

SDSU Data Science Symposium

Self-propelled sprayers are commonly used in agriculture to disperse agrichemicals. These sprayers commonly have two boom wings with dozens of nozzles that disperse the chemicals. Automatic boom height systems reduce the variability of agricultural sprayer boom height, which is important to reduce uneven spray dispersion if the boom is not at the target height.

A computational model was created to simulate the spray dispersion under the following conditions: a) one stationary nozzle based on the measured spray pattern from one nozzle, b) one stationary model due to an angled boom, c) superposition of multiple stationary nozzles due an angled boom, …


Trophically Integrated Ecometric Models As Tools For Demonstrating Spatial And Temporal Functional Changes In Mammal Communities, Rachel A. Short, Jenny L. Mcguire, P. David Polly, A. Michelle Lawing Feb 2023

Trophically Integrated Ecometric Models As Tools For Demonstrating Spatial And Temporal Functional Changes In Mammal Communities, Rachel A. Short, Jenny L. Mcguire, P. David Polly, A. Michelle Lawing

Natural Resource Management Faculty Publications

We are in a modern biodiversity crisis that will restructure community compositions and ecological functions globally. Large mammals, important contributors to ecosystem function, have been affected directly by purposeful extermination and indirectly by climate and land-use changes, yet functional turnover is rarely assessed on a global scale using metrics based on functional traits. Using ecometrics, the study of functional trait distributions and functional turnover, we examine the relationship between vegetation cover and locomotor traits for artiodactyl and carnivoran communities. We show that the ability to detect a functional relationship is strengthened when locomotor traits of both primary consumers (artiodactyls, n …


Refining The Moose Serum Progesterone Threshold To Diagnose Pregnancy, Madeline Struck, William J. Severud, Yvette M. Chenaux-Ibrahim, Edmund J. Isaac, Janine L. Brown, Seth A. Moore, Tiffany M. Wolf Feb 2023

Refining The Moose Serum Progesterone Threshold To Diagnose Pregnancy, Madeline Struck, William J. Severud, Yvette M. Chenaux-Ibrahim, Edmund J. Isaac, Janine L. Brown, Seth A. Moore, Tiffany M. Wolf

Natural Resource Management Faculty Publications

Pregnancy determination is necessary for sound wildlife management and understanding population dynamics. Pregnancy rates are sensitive to environmental and physiological factors and may indicate the overall trajectory of a population. Pregnancy can be assessed through direct methods (rectal palpation, sonography) or indicated using hormonal assays (serum progesterone or pregnancy-specific protein B, fecal progestogen metabolites). A commonly used threshold of 2 ng/ml of progesterone in serum has been used by moose biologists to indicate pregnancy but has not been rigorously investigated. To refine this threshold, we examined the relationship between progesterone concentrations in serum samples and pregnancy in 87 moose ( …


Evaluation Of Growth Performance, Energy Utilization, And Carcass Trait Responses Of Finishing Lambs Administered Zeranol, Emily Nold Jan 2023

Evaluation Of Growth Performance, Energy Utilization, And Carcass Trait Responses Of Finishing Lambs Administered Zeranol, Emily Nold

Schultz-Werth Award Papers

The objective of this research was to determine the influence of implanting heavy wether lambs with 12 mg zeranol (1 pellet Ralgro, Merck Animal Health. Madison, NJ) 59 d before harvest. Average daily gain (ADG), feed efficiency, and carcass merit were evaluated. Polpypay and crossbred wethers (n = 32 total) were equally divided into two treatment groups within a randomized complete block design. Sixteen pens were used resulting in 8 replicate pens per treatment. Wethers were fed a finishing diet consisting of cracked corn, soybean meal and soybean hulls ad libitum for 59 d. Lambs had access to clean water …


Whole Genome Sequencing For Identification Of Putative Causative Alleles For Hemochromatosis In Horses, Anna M. Rauber-Ramos Jan 2023

Whole Genome Sequencing For Identification Of Putative Causative Alleles For Hemochromatosis In Horses, Anna M. Rauber-Ramos

Schultz-Werth Award Papers

Hemochromatosis is liver disorder in which excess iron is stored in the liver, leading to iron overload and extensive liver damage that can prove fatal. Based on human precedence, a genetic cause for idiopathic hemochromatosis in horses is suspected. Two horses with hemochromatosis were whole genome sequenced, with variants in candidate genes prioritized in analyses of potential pathogenicity. No clear putative variants were found to be present in both horses, suggesting an etiology more complex than the initially suspected Mendelian inheritance. A heterozygous nonsense mutation in STEAP3 metalloreductase (STEAP3) was identified as a potential contributor to hemochromatosis in one of …


Kidney Stones And Energy Drinks: A Literature Review, Donovan D. Bentz Jan 2023

Kidney Stones And Energy Drinks: A Literature Review, Donovan D. Bentz

Schultz-Werth Award Papers

Kidney stones are common, painful, and expensive to treat. Several factors play a role in the development of stones, particularly the consumption of sugars such as fructose. As such, the consumption of sugary energy drinks contributes to the development of kidney stones. However, there is a lack of understanding behind the mechanism of kidney stone development and the role energy drinks play in stone development. This review analyzes the mechanism of kidney stone development and the effect energy drink consumption has on stone development. Here, we also detail the dietary roles calcium, oxalate, ascorbic acid, and animal protein have on …


Analyzing The Effects Of Winter-Patch Grazing And Wildfire On Insect Order Hymenoptera In The Northern Great Plains, Kaitlin Schieuer Jan 2023

Analyzing The Effects Of Winter-Patch Grazing And Wildfire On Insect Order Hymenoptera In The Northern Great Plains, Kaitlin Schieuer

Schultz-Werth Award Papers

Pollinators are declining globally, threatening global crop production and the biological integrity of many ecosystems. Hymenoptera (the order containing ants, bees, and wasps) is one of the most important insect orders for pollination of a variety of plants, including many crops, and is important as biocontrol for crop pests, and other herbivorous insects. Land management practices affect plant community composition, which influences vegetation-dependent insects, and consequently affects their ecosystem services. Fire and grazing are common practices on working landscapes in the Great Plains. However, how these management techniques impact insect diversity, particularly Hymenopterans, in a mixed-grass prairie ecosystem, is poorly …


Impacts Of Eastern Redcedar Juniperus Virginiana L. On Avian Communities In Eastern South Dakota, Jay Holm Jan 2023

Impacts Of Eastern Redcedar Juniperus Virginiana L. On Avian Communities In Eastern South Dakota, Jay Holm

Schultz-Werth Award Papers

Eastern redcedar (ERC) (Juniperus virginiana L.) is the most rapidly spreading woody species in the Northern Great Plains due to fire suppression, planting in windbreaks, and overgrazing. Its encroachment has converted already fragmented and/or degraded grassland into woodland, and there is concern that it may negatively impact grassland-obligate/dependent avian species. In this study, I evaluated avian community composition between ERC-encroached and non-encroached grasslands in eastern South Dakota. Six sites each of encroached grassland and non-encroached grassland were selected. Bird surveys were conducted by performing point counts placed equidistantly along a transect for two years. Presence and abundance of each species …


Diets Of Invasive Channel Catfish Are Subsidized By Invasive Riparian Trees, Christopher A. Cheek, Brandon K. Peoples, Reuben R. Goforth Jan 2023

Diets Of Invasive Channel Catfish Are Subsidized By Invasive Riparian Trees, Christopher A. Cheek, Brandon K. Peoples, Reuben R. Goforth

Natural Resource Management Faculty Publications

Russian olive (Elaeagnus angustifolia) is an invasive, fruit- bearing riparian tree that dominates riparian zones of the San Juan River in the southwestern United States. Previous research in this river suggests olive fruit is common in diets of invasive channel catfish (Ictalurus punctatus), but its energetic importance is unknown (i.e. critical for catfish fitness vs. incidental consumption). We assessed Russian olive consumption in channel catfish diets bimonthly for 1 year, hypothesizing that olive consumption would be greatest during periods of high olive availability and low benthic aquatic invertebrate availability. We found that catfish consumed olive fruit throughout the year and …


Weed-Induced Crop Yield Loss: A New Paradigm And New Challenges, David Horvath, Sharon A. Clay, Clarence J. Swanton, James V. Anderson, Wun S. Chao Jan 2023

Weed-Induced Crop Yield Loss: A New Paradigm And New Challenges, David Horvath, Sharon A. Clay, Clarence J. Swanton, James V. Anderson, Wun S. Chao

Agronomy, Horticulture and Plant Science Faculty Publications

Direct competition for resources is generally considered the primary mechanism for weed-induced yield loss. A re-evaluation of physiological evidence suggests weeds initially impact crop growth and development through resource-independent interference. We suggest weed perception by crops induce a shift in crop development, before resources become limited, which ultimately reduce crop yield, even if weeds are subsequently removed. We present the mechanisms by which crops perceive and respond to weeds and discuss the technologies used to identify these mechanisms. These data lead to a fundamental paradigm shift in our understanding of how weeds reduce crop yield and suggest new research directions …


Evaluation Of Yeast Postbiotic In Sow Diets On Sow And Offspring Performance And Microbial Succession, Joel Kieser Jan 2023

Evaluation Of Yeast Postbiotic In Sow Diets On Sow And Offspring Performance And Microbial Succession, Joel Kieser

Electronic Theses and Dissertations

Litter size and the resulting nutritional demand on the sow continue to increase while sow mortality and culling rate are also increasing. Non-nutritive feed additives may enhance sow health and thereby improve offspring growth and productivity after weaning. Development of the gut microbiome in piglets via microbial succession is critical for maximizing their productivity and providing stability for overcoming weaning stress. The objective of this study was to evaluate the impact of yeast-based postbiotic supplementation in gestation and lactation diets on offspring performance through the nursery period and on whether a yeast postbiotic could impact the sow fecal microbiome as …


Southeast South Dakota Experiment Farm Annual Progress Report, 2023, South Dakota Agricultural Experiment Station Jan 2023

Southeast South Dakota Experiment Farm Annual Progress Report, 2023, South Dakota Agricultural Experiment Station

Agricultural Experiment Station and Research Farm Annual Reports

This is an annual report of the research program at the Southeast South Dakota Research Farm in cooperation with South Dakota Agricultural Experiment Station and the SDSU College of Agriculture, Food, and Environmental Sciences and has special significance for those engaged in agriculture and the agriculturally related businesses in the ten county area of Southeast South Dakota. The results shown are not necessarily complete or conclusive. Interpretations given are tentative because additional data resulting from continuation of these experiments may result in conclusions different from those based on any one year.


The Effects Of Dexamethasone Administration On Physiological, Behavioral, And Production Parameters In Dairy Cows After A Difficult Calving, Turner H. Swartz, Dana Bryant, Hollie Schramm, Allison Duncan, Robin White, Cynthia Wood, Christina Petersson-Wolfe Jan 2023

The Effects Of Dexamethasone Administration On Physiological, Behavioral, And Production Parameters In Dairy Cows After A Difficult Calving, Turner H. Swartz, Dana Bryant, Hollie Schramm, Allison Duncan, Robin White, Cynthia Wood, Christina Petersson-Wolfe

Dairy Science and Food Science Faculty Publications: 2022 to Present

Dairy cows are predisposed to diseases during the postpartum period. Dystocia has been associated with increased risk for disease, which is likely the result of increased tissue trauma and stress during the prolonged parturition. To attenuate the inflammatory response seen in dystocic animals and improve well-being, we assessed the effects of a glucocorticoid, dexamethasone administered within 12 h after calving. Dystocia was defined as a difficult birth resulting in a prolonged calving (≥70 min after the amniotic sac appears) and was monitored through 3 video cameras in the close-up dry-cow pen. Cows meeting the dystocia definition were randomly …


Cover Crop Composition In Long-Term No-Till Soils In Semi-Arid Environments Do Not Influence Soil Health Measurements After One Year, Hunter Bielenberg, Jason D. Clark, Debankur Sanyal, Johnathon Wolthuizen, David Karki, Amin Rahhal, Anthony Bly Jan 2023

Cover Crop Composition In Long-Term No-Till Soils In Semi-Arid Environments Do Not Influence Soil Health Measurements After One Year, Hunter Bielenberg, Jason D. Clark, Debankur Sanyal, Johnathon Wolthuizen, David Karki, Amin Rahhal, Anthony Bly

Agronomy, Horticulture and Plant Science Faculty Publications

Evaluating the influence of grass or broadleaf cover crops on soil health measurements is common in the northern US Midwest. However, the comparison among different cover crop mixtures, including blends of both grass and broadleaf species is limited. In 2018–2020, cover crop experiments were conducted in South Dakota at 11 site-years. Cover crops were planted in the summer after small grains harvest as mixtures of dominantly grasses or broadleaves, a 50/50 grass/broadleaf mixture, and a no cover crop control. Soil and above-ground plant residue samples were collected in the fall before winter termination and in the spring before corn planting. …


Regional Assessment Of Live Cattle And Carcass Characteristics In The Northern Plains, Harlee A. Brauckmuller Jan 2023

Regional Assessment Of Live Cattle And Carcass Characteristics In The Northern Plains, Harlee A. Brauckmuller

Electronic Theses and Dissertations

The goal of this project was to perform a regional assessment of live cattle and carcass characteristics in the northern plains. The assessment audited three packing plants to assess fed cattle, cow, and bull production. The live animal factors evaluated were transportation, mobility of cattle, identification, brands, and mud/manure present. Data collected during the slaughter process was bruising, dentition, and offal condemnations. In fed cattle yield grade, quality grade, and hot carcass weight were assessed. In cows and bulls muscle score and fat color score were assessed. To assess differences between cattle in the Northern Plains and cattle in the …


Characterizing Vaginal Gene Expression In Prepubertal Gilts, Shannon Lee Dierking Jan 2023

Characterizing Vaginal Gene Expression In Prepubertal Gilts, Shannon Lee Dierking

Electronic Theses and Dissertations

The best-known predictor of reproductive success in gilts is the age at puberty. Early puberty (i.e., d170 – d180 of age) is associated with improved long-term reproductive performance and more full value offspring for market. The ability to predict, in the prepubertal stage, the age that a replacement gilt would be expected to achieve puberty has great reproductive and economic advantages. This work focuses on identifying biological markers that are potentially predictive of age at puberty. Study 1 identified changes in the vaginal epithelium during gilt reproductive development. Pre-pubertal gilts (n =13) were followed from d70 of age until first …


Unveiling Race Diversity And Fungicide Sensitivity Profiles Of Pyrenophora Tritici Repentis Populations In South Dakota Collected From 2021-2023, Jaswinder Kaur Jan 2023

Unveiling Race Diversity And Fungicide Sensitivity Profiles Of Pyrenophora Tritici Repentis Populations In South Dakota Collected From 2021-2023, Jaswinder Kaur

Electronic Theses and Dissertations

Tan spot, a foliar disease of wheat caused by the ascomycetes fungus Pyrenophora tritici repentis (Ptr), poses a significant threat to wheat crops globally. The disease gained economic importance in the late 1970s due to shifts in farming practices, such as minimal tillage and planting on crop residues, providing favorable conditions for the pathogen to overwinter and cause infections. Ptr is classified into eight races based on the production of necrotrophic effectors—Ptr ToxA (necrosis-inducing), Ptr ToxB, and Ptr ToxC (chlorosis-inducing on respective susceptible and sensitive cultivars)—resulting in distinct symptoms on susceptible wheat differentials. The inverse gene-for-gene hypothesis of Ptr suggests …


Evaluation Of Feed Additives And Delivery Method For Receiving And Finishing Cattle, Thiago Lauro Maia Ribeiro Jan 2023

Evaluation Of Feed Additives And Delivery Method For Receiving And Finishing Cattle, Thiago Lauro Maia Ribeiro

Electronic Theses and Dissertations

Trace minerals (TM) and vitamins are essential to improve production output and efficiency in beef cattle. The first experiment was conducted to investigate if the delivery method of TM and Saccharomyces cerevisiae yeast culture influence growth performance and hepatic TM content of receiving steers. The second experiment evaluated the effect of supplemental protected B - vitamins to finishing steers. Experiment 1 used Charolais x Angus steers calves (n = 192; 256 ± 14 kg) in a 49-d receiving experiment. Within 36h of weaning, steers were weighed, allotted to 24 pens (n = 8 steers/pen; 8 pens/treatment) and randomly assigned to …


Extraction, Characterization, And Utilization Of Yellow Pea Starch, Abdulmalik Albu Tuwaybah Jan 2023

Extraction, Characterization, And Utilization Of Yellow Pea Starch, Abdulmalik Albu Tuwaybah

Electronic Theses and Dissertations

Dry peas, a leguminous crop, are one of the world's oldest crops, accounting for 35–40% of total pulse trade. Pea starch is an inexpensive source of starch since it can be obtained as a by-product of protein extraction. Recovering the starch from protein isolation streams (i.e., before or after) improves the environmental impact of the protein isolation process. The goal of the study was to transform low-value pea starch into highvalue starch through starch extraction and food application. Two methods of starch extraction were used (47.5% ethanol treatment and SC CO2 + EtOH extraction). Starch samples were analyzed for chemical, …


Facing Into The Blizzard: Resiliency And Mortality Of Native And Domestic North American Ungulates To Extreme Weather Events, Jeff Martin Jan 2023

Facing Into The Blizzard: Resiliency And Mortality Of Native And Domestic North American Ungulates To Extreme Weather Events, Jeff Martin

Natural Resource Management Faculty Publications

Unseasonably early blizzards in the northern Great Plains threaten large mammal populations unacclimated for variable and extreme winter conditions. This region averaged 22 blizzards per winter season during the 2010s, up from 6 during the 1960s, and is anticipated to average 32 blizzards by the 2050s. In early October 2013, the fatal Atlas Blizzard affected four livestock and captive species in 16 counties of western South Dakota. Expected one-week total death losses for the study area were estimated from national average background mortality rates: 161 cattle, 102 sheep, 9 horses, and 6 bison. However, observed death loss varied significantly (McNemar’s …


In-Vivo Assessment Of A Direct-Fed Microbial On Lactation Performances, Blood Biomarkers, Ruminal Fermentation And Microbial Abundance In Transition To Mid-Lactation Holstein Cows, Marcela Bulnes Lopez Jan 2023

In-Vivo Assessment Of A Direct-Fed Microbial On Lactation Performances, Blood Biomarkers, Ruminal Fermentation And Microbial Abundance In Transition To Mid-Lactation Holstein Cows, Marcela Bulnes Lopez

Electronic Theses and Dissertations

The transition period is a crucial stage in the lactation cycle and can lead to significant metabolic changes in cows. The use of nutritional interventions, such as direct-fed microbials, may assist cows during the transition from pregnancy to early lactation, reducing the occurrence of metabolic disorders and improving overall health. The primary goal of this study was to assess the effects of a commercial rumen-derived directfed microbial (DFM) product (Galaxis™ Frontier (GF) Native Microbials Inc., CA, USA) on various factors such as performance, blood biomarkers, rumen fermentation, and bacterial population in dairy cows during the transition period up to 100 …


Fungicide Sensitivity In P. Tritici-Repentis Diverse Population And Phenotyping Of Spelt Wheat For Multiple Diseases, Zunera Shabbir Jan 2023

Fungicide Sensitivity In P. Tritici-Repentis Diverse Population And Phenotyping Of Spelt Wheat For Multiple Diseases, Zunera Shabbir

Electronic Theses and Dissertations

Pyraclostrobin, a QoI fungicide, is extensively used in the management of fungal leaf spot diseases. In this study, 215 P. tritici-repentis isolates collected from wheat fields in South Dakota were evaluated for their sensitivity to Pyraclostrobin. Of 215 isolates, 48 isolates (22%) exhibited insensitivity based on G143A mutation and EC50 values using spore germination assay. Our results indicate reduced sensitivity to insensitivity to pyraclostrobin in some isolates suggesting regular monitoring of the P. tritici-repentis population to QoI fungicides is essential to track the evolution of insensitive population. Spelt wheat (Triticum spelta L.) is an ancient cereal that is gaining small …


Indigenous Toxin Affects Cell Viability, While An Artificial Proteolytic Queueing Causes The Upregulation Of Specific Genes, Mohammed Salahuddin Jan 2023

Indigenous Toxin Affects Cell Viability, While An Artificial Proteolytic Queueing Causes The Upregulation Of Specific Genes, Mohammed Salahuddin

Electronic Theses and Dissertations

Studying bacterial physiology is crucial to understand the fundamental mechanisms that govern bacterial growth, survival, and adaptation. This thesis combines two chapters investigating bacterial physiology by studying important cellular processes like bacterial toxin-antitoxin systems and proteolytic pathways. The first chapter involves studying bacterial toxin-antitoxin systems to understand the regulation and function of hypothetical toxin-antitoxin (TA) systems of bacteria and the effects of these TA systems on bacterial growth and survival. The emergence of these genetic modules in bacterial research and the unrevealing of some of their important roles in cell physiology in recent years has drawn much attention in scientific …