Veterinary Preventive Medicine, Epidemiology, and Public Health Commons^™

Multidrug Resistance, Extended-Spectrum Β-Lactamase (Esbl) Production, And Colistin Resistance In Escherichia Coli From Retail Chicken Meat In Fresh Markets In Bangkok, Thailand, Htet Lin Oo, Nawaphorn Roongrojmongkhon, Suwanee Chalermchainukul, Sirawit Pagdepanichkit

The Thai Journal of Veterinary Medicine

Contamination of chicken meat with antimicrobial-resistant bacteria, particularly multidrug-resistant (MDR), colistin-resistant, and extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (E. coli), threatens its safety for human consumption. This study was conducted to determine the prevalence and antimicrobial resistance (AMR) profiles, particularly ESBL production and colistin resistance in E. coli isolated from retail chicken meat in fresh markets in Bangkok, Thailand. A total of 200 (81.6%) E. coli isolates were recovered from 245 chicken meat samples collected from fresh markets across six regions of Bangkok. Among the isolates, 189 (94.5%) exhibited resistance to at least one antimicrobial agent tested, with the …

Molecular Detection Of Chlamydiales In Captive Zoo Animals At Songkhla Zoo, Southern Thailand, Rawikan Inchuai, Sunisa Khongthong, Boontarikaan Wititkornkul, Naruepan Wongpluksin

The Thai Journal of Veterinary Medicine

Several species of Chlamydia are recognized as significant zoonotic pathogens that infect a wide range of hosts, including humans. This study aimed to investigate the occurrence and genetic characteristics of Chlamydiales–related bacteria in captive animals at Songkhla Zoo, southern Thailand. A total of 92 cloacal and rectal swab samples were collected from mammals (n = 22), birds (n = 55), and reptiles (n = 15). Genomic DNA was screened using polymerase chain reaction (PCR) targeting the Chlamydiales 16S rRNA gene and the ompA gene specific for Chlamydia psittaci. Positive amplicons were subjected to Sanger sequencing and phylogenetic analysis. A …

Prevalence And Risk Factors Association Of Babesiosis In Cattle And Buffaloes In District Naushahro Feroze, Sindh, Pakistan, Kazim Raza, Amjad Hussain, Sajid Moin, Huzaifa Arain, Muhammad Mohsen, Maria Abubakar, Erum Bughio, Barkat Ali

Journal of Bioresource Management

Background: Bovine babesiosis is a protozoan disease that is transmitted by ticks and has significant veterinary and economic impacts on tropical livestock systems. Prior to this study, no epidemiological data was available of Sindh, Pakistan.

Purpose: To estimate the Babesia spp. prevalence and estimate the risk factors with respect to cattle and buffaloes in five tehsils of District Naushahro Feroze, Sindh, Pakistan.

Methods: A cross-sectional study was conducted using 200 blood samples (100 Thari cattle; 100 Kundhi buffaloes; both sexes) in five tehsils (Kandiaro, Moro, Mehrabpur, Bhiria and Naushahro Feroze). Thin blood smears under Giemsa stain were viewed under oil-immersion …

Current Status Of Anthelmintic Resistance In Meat Goats: A Comparative Efficacy Study Of Albendazole And Fenbendazole In Maha Sarakham, Thailand, Minta Pipatsakulpak, Usanisa Boonum, Kotchakorn Roongruang, Benjawan Sae-Chue, Sukanya Leethongdee, Songsak Chumpawadee, Nattaya Watwiengkam, Pongphol Pongthaisong

The Thai Journal of Veterinary Medicine

Anthelmintic resistance in gastrointestinal nematodes threatens sustainable goat production globally. While resistance is well-documented in many regions, data from Thailand remain limited. This study determined the prevalence of gastrointestinal nematodes and assessed the efficacy of albendazole and fenbendazole in meat goats in Maha Sarakham province, northeastern Thailand. A two-stage cross-sectional study was conducted on 10 meat goat farms between August 2021 and March 2022. In Stage 1, fecal samples from 150 goats were examined using simple flotation and sedimentation techniques to identify parasite morphology. In Stage 2, the McMaster technique was employed to quantify fecal egg counts (FEC). Twenty-six goats …

Understanding Vaccine Hesitancy In Veterinary Medicine: A Review Of Client Attitudes, Grace Moore

Undergraduate Honors Capstone Projects

Uncertainty toward vaccination, long recognized as a public health concern in human medicine, is increasingly recognized within veterinary contexts, posing risks to both animal and community health. This literature review examines the development, causes, and implications of reduced vaccine confidence in veterinary medicine, with emphasis on small animal and some large animal practices. The review combines historical and current research to explore how vaccination perceptions have evolved among veterinarians and clients, and how factors such as the COVID-19 pandemic have shaped current attitudes.

The literature highlights recurring themes surrounding vaccine reluctance in veterinary settings. Client perceptions of safety, efficacy, and …

Risk Factors Of Leptospirosis Among Agricultural Workers In Southeast Asia: Literature Review, Ronggo Adi Wibowo, Wawa Najwa Nurzakia, Meirany Sasitha Kirana, Yulia Arthemisia Wijaya, Jasmine Ayumaharani Sekaryudha, Dwi Sarwani Sri Rejeki, Rosita Dwi Jayanti

Jurnal Epidemiologi Kesehatan Indonesia

The agricultural sector is the primary source of livelihood for communities in Southeast Asia; however, these activities increase the risk of exposure to zoonotic diseases, particularly leptospirosis. Southeast Asia is one of the regions with the highest incidence of leptospirosis in the world. This literature review aims to identify risk factors for leptospirosis among agricultural workers in Southeast Asia. The search for research articles utilized three databases (Google Scholar, PubMed, and ScienceDirect) using a descriptive approach based on literature review and the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. A total of 9,800 articles were screened for …

Epidemiologic Evaluation Of Canine Urate Urolithiasis In Thailand: A Comparative Cross-Sectional Study Between 2016-2023, Nattaporn Chaysakul, Jody P. Lulich, Vachira Hunprasit

The Thai Journal of Veterinary Medicine

A comparative cross-sectional study aimed to describe the epidemiological data of canine urate urolithiasis in Thailand by evaluating the change in proportion over the year and identifying risk-associated factors, including age, breed, sex, and neutered status, through comparisons with multiple comparison groups. The urate and non-urate group was dogs whose uroliths were submitted from Thailand to the Minnesota Urolith Center between 2016 and 2023, and the hospital group was dogs that were visited at the Small Animal Hospital, Chulalongkorn University, between 2020 and 2023. Of 6,171 canine urolith submissions, 210 samples (3.4%) were urate-containing uroliths submitted from twenty-eight canine breeds. …

Surveillance Of A Novel Vesivirus Discovered In An Urban Mephitis Mephitis Population, Amanda E. Diggins

Honors Theses and Capstones

Caliciviridae is a family of non-enveloped, positive-sense, single stranded RNA viruses, including eleven defined genera that contain important pathogens of humans and animals. Within the Caliciviridae family, the genus Vesiviridae includes feline calicivirus, a leading cause of upper respiratory disease in cats, and vesicular exanthema of swine virus, a historical disease of domestic swine that has now been implicated in several marine mammal vesicular diseases, one of which has been confirmed zoonotic. Urban landscapes create a unique environment for rapid intra- and interspecies transmission of viruses. Clustered resources attract high-density populations of synanthropic wildlife species, increasing spatial overlap and heightening …

Effects Of Replacing Antibiotics With Bacillus-Based Probiotics In The Diet Of Lactating Sows On Reproductive Performance, Feed Intake, Serum Immunoglobulin Levels, Milk Production, Oxidative Stress Markers, Fecal Short-Chain Fatty Acids, And Piglet Growth Performance, Chutikan Srisang, Tip-Apa Akkhaphan, Preechaphon Taechamaeteekul, Junpen Suwimonteerabutr, Rafa Boonprakob, Suparlark Nuntawan Na Ayudhya Dr, Lea Hübertz Birch Hansen Dr, Padet Tummaruk

The Thai Journal of Veterinary Medicine

This clinical trial investigated the effects of adding a Bacillus-based probiotic to the lactation diet of sows (without in-feed antibiotics) on maternal and piglet outcomes. A total of 109 Landrace × Yorkshire sows (parity 1–7) were assigned to either a control group (n = 61) or a treatment group (n = 48) receiving Bacillus subtilis – 541 and Bacillus amyloliquefaciens – 516. Piglets in the treatment group also received the probiotics via creep feed from day 3 until weaning. Blood samples were collected within 12 h after farrowing to assess serum immunoglobulins (IgG, IgA, and IgM) and malondialdehyde (MDA) …

Seroprevalence Of Small Ruminant Lentiviruses (Srlvs) And Peste Des Petits Ruminants Virus (Pprv) In Nonvaccinated Sheep And Goats In Sarawak, Malaysia, Bura Thlama Paul, Ali Saidu, Maria Eva Barudi, Faez Firdaus Abdullah Jesse, Juriah Kamaludeen, Suhaili Mustafa, Masnindah Malahubban, Ali Hanafiah Hakim, Krishnan Nair Balakrishnan, Mohd Azmi Mohd Lila

The Thai Journal of Veterinary Medicine

Small ruminant lentiviruses (SRLVs) and Peste des Petits Ruminants virus (PPRV) are economically important viral diseases that threaten small ruminant production globally. While previous studies have reported their serological status in Peninsular Malaysia, there is limited epidemiological information on their occurrence in East Malaysia. This study aimed to determine the seroprevalence and risk factors of SRLVs and PPRV among nonvaccinated sheep and goats from selected smallholder farms in Sarawak. A total of 245 serum samples collected from sheep (n = 117) and goat (n = 128) were screened using commercial ID Screen^® PPR competition (PPRC ver. 0821) and MVV/CAEV …

Bacterial Identification And Antibiotic Sensitivity Profiles From Captive Birds At Taipei Zoo, Younjung Chang, I-Li Liu, Jane-Fang Yu, Lih-Chiann Wang

The Thai Journal of Veterinary Medicine

Bacterial infections are common in zoo birds. They may be caused by pathogenic bacteria or opportunistic bacteria due to trauma or stress-induced immune compromise. Improper use of antibiotics contributes to the development of resistant bacteria, making treatment complicated or ineffective. However, comprehensive clinical investigations into bacterial prevalence and antibiotic susceptibility in zoo-captive birds remain limited. This study identified the bacterial species present in captive diseased birds at the zoo and conducted a 12-year analysis of their antibiotic susceptibility profiles. The specimens from 48 birds at Taipei Zoo from 2006 to 2017 were submitted to a commercial reference laboratory for bacterial …

Possible Cross-Resistance And Horizontal Gene Transfer Associated With Halquinol In Escherichia Coli And Salmonella Enterica Isolated From Pigs, Nisit Chansong, Songsak Srisanga, Nutthavut Mahawatthanaunkul, Rungtip Chuanchuen

The Thai Journal of Veterinary Medicine

The aims of this study were to determine the possible cross-resistance and horizontal gene transfer associated with halquinol in Escherichia coli and Salmonella spp. from pigs. Two of each E. coli and Salmonella isolates exhibiting low halquinol MIC (8 and 16 µg/ml, respectively) and susceptible to antibiotics tested were selected for in vitro stepwisehalquinol exposure experiment. Another E. coli (n=19) and Salmonella (n=6) isolates with high halquinol MICs (16-64 and 32-64 µg/ml, respectively) containing plasmids were used for transferability testing of halquinol resistanceencoding gene(s) using filter mating method. In vitro exposure to subinhibitory concentrations of halquinol promoted neither resistance to …

Genetic Characterization Of Lpai-H4n2 And H4n8 Viruses Recovered From Free-Grazing Ducks In Thailand, Supanat Boonyapisitsopa, Supassama Chaiyawong, Kamonpan Charoenkul, Kitikhun Udom, Ekkapat Chamsai, Napawan Bunpapong, Somsak Pakpinyo, Alongkorn Amonsin Prof. Dr.

The Thai Journal of Veterinary Medicine

nfluenza A viruses (IAVs) can infect birds and various mammals. In this study, we retrospectively isolated and characterized IAVs (n=8) from oropharyngeal and cloacal swab samples of free-grazing ducks collected during December 2018 to December 2021, by using egg inoculation and whole-genome sequencing. The IAVs could be subtyped as IAV-H4N2 (n = 3) and IAV-H4N8 (n = 5) using real-time RT-PCR assays. Genetic and phylogenetic analyses revealed that all eight genes of Thai IAV-H4 belong to the Avian Eurasian lineage. Both Thai IAV-H4N2 and IAV-H4N8 exhibited low pathogenic avian influenza (LPAI) characteristics and were susceptible to oseltamivir and amantadine. This …

Prevalence, Antimicrobial Resistance Characteristics And Virulence Genes Of Streptococcus Suis In Pigs In Upper Northern Thailand, Wanida Mala, Rangsiya Prathan, Si Thu Hein, Sunpetch Angkittitrakul, Asinamai Athliamai Bitrus, Rungtip Chuanchuen

The Thai Journal of Veterinary Medicine

This study aimed to investigate the prevalence, virulence genes and antimicrobial resistance of Streptococcus suis from pigs in Upper-Northern Thailand. A total of 768 nasal swabs were obtained from pigs at the municipal slaughterhouses in five provinces including Phayao (n=202), Nan (n=190), Chiang Rai (180), Chiang Mai (n=130), and Mae Hong Son (n=66) in 2018-2019. The prevalence of S. suis varied from 3.0%-9.4%, of which the highest prevalence was in Chiang Rai (9.4%) and Mae Hong Son (9.1%). Of all 59 isolates confirmed to be S. suis, serotype 8 (50.8%) was most commonly identified, followed by serotype 10 (3.4%), …

Digestibility, Palatability, And Nutrient Composition Of Three Species Of Whole Prey In Three Species Of Snakes, Morgan R. Kienzle

Department of Animal Science: Dissertations, Theses, and Student Research

Snakes in human care often lack diversity in the diet. Rodents account for the majority of meals, while wild snakes are consuming a varied diet composed of multiple different whole prey species. By feeding only one species of prey, the chance of nutrient deficiencies and other health concerns increases. To promote variety in the managed diet, two commercially available whole prey species (mice (Mus musculus) and chicks (Gallus spp.)) and one novel species (Seba’s short-tailed bats (Carollia perspicillata)), were analyzed for their dry matter (DM), organic matter (OM), crude protein (CP), crude fat …

The Survey Of The Prevalence Of Equine Gastric Ulcer Syndrome (Egus) In Non-Racing Thoroughbred Horses In Thailand, Teerapol Tum Chinkangsadarn, Sangtawan Tubkleaw, Ananya Rungjarusub, Jitsupa Trangkatarn, Kawinthida Thaworn, Parichart Tesena

The Thai Journal of Veterinary Medicine

This study aimed to determine the prevalence, types, and risk factors of Equine Gastric Ulcer Syndrome (EGUS) in non-racing Thoroughbred horses in Thailand. A total of 56 Thoroughbreds, aged 5 to 22 years (mean age: 12.43 ± 4.65 years), were included. Before sedation, a comprehensive history and physical examination were conducted. Gastroscopy was performed to identify gastric ulcers, using the Equine Gastric Ulcer Council 0–4 scoring system for non-glandular ulcers, while glandular ulcers were assessed as present or absent. Data on breed, age, sex, activity status, behavior, appetite, and medical rehistory were collected. The overall prevalence of gastric ulcers was …

Effect Of Feeding Fenugreek Extract And Encapsulated Lecithin On Finishing Cattle Performance, Carcass Characteristics, And Liver Abscesses, Sofia Suarez Lorences, Rebecca L. Mcdermott, Joshua R. Benton, Robert Bondurant, Willard Lemaster, James C. Macdonald, Galen E. Erickson

Nebraska Beef Cattle Reports

Summary with Implications

A feedlot study was conducted to evaluate the effects of adding fenugreek extract and encapsulated lecithin (Furst-McNess Company, Rockford, Illinois, United States) on finishing cattle performance, carcass characteristics, and liver abscesses. Treatments were applied as a 2×2 factorial that included a control diet with no additive; a diet containing fenugreek extract; a diet containing encapsulated lecithin; and a diet containing both fenugreek extract and encapsulated lecithin. Tylosin was not included in any diets to test natural occurrence of liver abscesses. No interactions were observed between fenugreek extract and encapsulated lecithin treatments but neither feed additive impacted intakes, …

Effects Of Allocation Frequency On Cattle Performance And Forage Utilization When Swath Grazing A Sorghum-Sudangrass Hybrid In Eastern Nebraska, Thomas E. Aquino, Mary E. Drewnoski, Pedro Henrique J. Fernandes

Nebraska Beef Cattle Reports

Summary with Implications

A sorghum-sudangrass hybrid was swath grazed by growing steers (529 ± 18 lb) from November 2023 to January 2024. Treatments were offering new forage once (1X) or twice (2X) per week to determine the effect of allocation frequency on animal performance and forage utilization. Average daily gain was not impacted by treatment (1X = 0.45 ± 0.1 lb/day, 2X = 0.45 ± 0.1 lb/day). Pre-and post-graze biomass samples were collected to estimate forage utilization, which was not different between treatments (1X = 57.1 ± 3.6%, 2X = 61.1 ± 3.6%). However, there was a difference in carrying …

Impact Of Overwinter Gain On Growth And Reproductive Performance In March-Born Heifers, Josie N. Crouch, J. Travis Mulliniks, Jacki A. Musgrave, Kacie L. Mccarthy

Nebraska Beef Cattle Reports

Summary with Implications

Heifers developed to lower rates of overwinter gain may reduce development costs without growth and reproduction impacted. A 3-year study was conducted to evaluate different overwinter rates of gain on heifer growth rates, reproductive, and economic performance. Heifers were managed together and individually supplemented (January-May) with dried distillers grains to achieve either a low (0.75 lb/d) or high (1.50 lb/d) average daily gain. At breeding, high gain heifers were heavier than low gain heifers. Average daily gain and body condition score were similar between treatments. Overwinter treatment did not impact attainment of puberty, pregnancy rates, or the …

Impact Of Pre- And Post-Breeding Supplementation On Performance Of May-Born Heifers, Josie N. Crouch, J. Travis Mulliniks, Jacki A. Musgrave, Kacie L. Mccarthy

Nebraska Beef Cattle Reports

Summary with Implications This 3-year study compared growth, reproductive performance, and economics of May-born yearling heifers that either received 2.00 lb/ heifer/ per d of a dried distillers grain supplement from pre-breeding (July) through breeding (August) or no supplement. Supplementation was offered approximately 24 days before breeding, averaging 55 days total. Heifer body weight was unaffected by supplementation. Supplementation increased average daily gain until pregnancy diagnosis, but no differences were noted after pregnancy diagnosis. Providing dried distillers grain increased the percentage of heifers that were cycling before breeding; however, no differences in pregnancy rates, percent that gave birth in the …