Veterinary Preventive Medicine, Epidemiology, and Public Health Commons^™

Genetic Tracking Of A Rabid Coyote (Canis Latrans) Detected Beyond A Rabies Enzootic Area In West Virginia, Usa, Matthew W. Hopken, Crystal Gigante, Amy T. Gilbert, Richard B. Chipman, Jordana D. Kirby, Rene Edgar Condori, Samuel Mills, Chelsea Hartley, John Forbes, Lisa Dettinger, Dongxiang Xia, Yu Li, Bridgett Vonholdt

United States Department of Agriculture Wildlife Services: Staff Publications

Wildlife translocation and cross-species transmission can impede control and elimination of emerging zoonotic diseases. Tracking the geographic origin of both host and virus (i.e., translocation versus local infection) may help determine the most effective response when high-risk cases of emerging pathogens are identified in wildlife. In May 2022, a coyote (Canis latrans) infected with the raccoon (Procyon lotor) rabies virus variant (RRV) was collected in Lewis County, West Virginia, USA, an area free from RRV. We applied host population genomics and RRV phylogenetic analyses to determine the most likely geographic origin of the rabid coyote. Coyote …

Statistics Used In The Nebraska Beef Reports And Their Purpose, University Of Nebraska-Lincoln

Nebraska Beef Cattle Reports

The purpose of beef cattle and beef product research at UNL is to provide reference information that represents the various populations (cows, calves, heifers, feeders, carcasses, retail products, etc) of beef production. Obviously, the researcher cannot apply treatments to every member of a population; therefore he/she must sample the population. The use of statistics allows the researcher and readers of the Nebraska Beef report the opportunity to evaluate separation of random (chance) occurrences and real biological effects of a treatment. Following is a brief description of the major statistics used in the beef report. For a more detailed description of …

Evaluation Of Vivalto® On Growth Performance And Carcass Characteristics In Growing And Finishing Beef Steers, Garrett N. Lemon, Rebecca Mcdermott, Galen E. Erickson, Jeff Heldt, Pablo L. Loza

Nebraska Beef Cattle Reports

A finishing trial conducted at the Pan-handle Research, Extension, and Education Center (PREEC) near Scottsbluff, NE evaluated the effect of Vivalto^®, a B-vitamin complex, on the feeding performance and carcass characteristics of feedlot steers. The design of the study was completely randomized block design with three treatments, control without Vivalto^®, 1g Vivalto^®/steer/day, and 2g Vivalto^®/steer/day. Cattle fed the diets that included Vivalto^® feed had lower intakes during the first 56 days than cattle that received no Vivalto^® in the diet. No significant differences were observed for ADG, intake, feed efficiency …

Effect Of Biochar On Enteric Methane Production And Cattle Performance, Hanna Cronk, Holly Heil, Rebecca Mcdermott, Mitch Norman, Galen E. Erickson, Andrea Watson

Nebraska Beef Cattle Reports

Methane is a more potent greenhouse gas than carbon dioxide. Because ruminant animals, including cattle, emit methane, strategies are actively being sought to reduce these emissions. Pistachio shell-sourced biochar was included at 0.5% of a finishing cattle diet to determine effects on methane emissions and cattle performance. Eight pens of cattle were rotated through a 2-chambered emissions barn to analyze methane and carbon dioxide produced by the cattle. Biochar tended to increase methane emissions on a g/d basis with no effect on methane emissions as g/lb of feed intake and no differences in carbon dioxide emissions. There were no differences …

Impact Of Strip-Grazing Stockpiled Annual/Cover Crop Forages On Carrying Capacity And Animal Performance, Shelby L. Davies-Jenkins, Abigail Sartin, Devin Jakub, Zac Carlson, Erin Laborie, Jack Arterburn, Ben Beckman, Brad Schnick, M. E. Drewnoski

Nebraska Beef Cattle Reports

Annual forages/cover crops can be used to fill the fall/winter grazing gap, and strip grazing may increase carrying capacity by reducing trampling losses of the forage. The current experiment utilized a series of on-farm experiments across two growing seasons to compare continuous and strip grazing of various summer planted cover crops. Strip grazing increased carrying capacity by an average of 47 ± 15% and gain per acre by 44 ± 5% compared to continuous grazing although significant variability in the amount of increase was observed. This variability can likely be attributed to forage type (quality), frequency of moves, and forage …

Strategies For Ddgs Supplementation Frequency To Frazing Yearling Streers, Sydney T. Vanderhoff, Rebecca L. Sjostrand, Mitchell M. Norman, Isaque F. Vicci, Jim C. Macdonald, Mary E. Drewnoski

Nebraska Beef Cattle Reports

Infrequent supplementation saves labor and may reduce animal performance, but recent research with reduced fat distillers grains has provided inconsistent results. This experiment evaluated the effects of daily and three times a week supplementation of dried distillers grains on yearling steer performance grazing smooth bromegrass pastures from May to August. Daily supplemented steers received 5.6 pounds of dry matter per steer of dried distillers grains with solubles 7 days/week. The three times a week supplemented steers received 13.0 pounds of dry matter per steer of dried distillers grains with solubles three days/week (Monday, Wednesday, Friday). A control treatment received no …

Effect Of Methionine Supplementation During Late Gestation In Beef Females, Landon F. Tadich, Jim R. Teichert, Kathryn J. Hanford, Jacki A. Musgrave, Rich N. Funston

Nebraska Beef Cattle Reports

Some amino acids are known to be essential to cattle and effect protein availability to the animal, especially during gestation when nutrient requirements are higher. Methionine is found to be one of the most limiting in low quality forage diets. Two 3-yr studies were performed to evaluate the impact of methionine supplementation during late gestation on intake, body weight, average daily gain, and subsequent calf performance in primiparous and multiparous females. In exp 1, 120 artificially inseminated pregnancy heifers were placed in a Calan gate feeding system (n=40/yr) and assigned 1 of 3 treatments during late gestation and fed ad …

Artificial Insemination Of Beef Heifers With Multi-Sire Semen, Dempster Christenson, Jordan Thomas, Daniel J. Kelly, John G. Maddux, R. Funston

Nebraska Beef Cattle Reports

The study compared pregnancy rates of beef heifers artificially inseminated with multi-sire semen to single-sire semen at ranch 1 and demonstrated pregnancy to multi-sire sexed semen at rand 2. It was hypothesized pregnancy rates resulting from multi-sire semen would be increased compared to single-sire semen. Ranch 1 heifers were inseminated with either single-sire or multi-sire semen and all heifers expressing estrus at ranch 2 were inseminated with multi-sire semen averaged numerically greater pregnancy rate than the average single-sire pregnancy rate and pregnancy outcomes from multi-sire sexed semen exceed previous literature but cannot be directly compared. Despite similar pregnancy results between …

Long-Term Performance Of Steers Grazing Smooth Bromegrass Pastures, Morgan Hodges, Holly Heil, Rebecca Mcdermott, Anastasios Mazis, Tala Awada, Galen E. Erickson, Andrea K. Watson

Nebraska Beef Cattle Reports

Summary with Implications

Body weight gain of yearling steers grazing smooth bromegrass pastures was measured across 17 years from 2005 to 2021. Three treatments were applied, (1) control pastures with no additional inputs, (2) pastures fertilized with 80 lb of N per acre, and (3) pastures grazed with cattle supplemented daily with approximately 5 lb of dry distillers grains. The cnntrol treatment pastures (1.4 acres/steer) were stocked 30% lighter than the fertilized and supplemented pastures (1.0 acre/steer). Across the 17 years, the supplemented cattle gained 2.24 lb/day while the non-supplemented cattle (both control and fertilized treatments) gained 1.57 lb/day. Body …

Treatment With The Immunocontraceptive Vaccine, Gonacon, Induces Temporary Fertility Control In Free-Ranging Prairie Dog Populations In Colorado, Usa, Aaron B. Shiels, Jackson Runte, Emily W. Ruell, Douglas C. Eckery, Gary W. Witmer, Daniel J. Salkeld

United States Department of Agriculture Wildlife Services: Staff Publications

Context

Prairie dogs (Cynomys spp.) are rodents enjoyed by some humans; yet, they cause crop and property damage, and carry zoonotic disease. Non-lethal control of prairie dogs is of interest in urban/suburban settings. The injectable immunocontraceptive vaccine GonaCon (active ingredient is gonadotropin-releasing hormone [GnRH]) has been shown to be effective at reducing fertility in equine and deer, and is a US EPA-registered vaccine for use in these ungulate species.

Aims

To conduct a replicated field study to test efficacy of GonaCon in black-tailed prairie dogs (C. ludovicianus), and if found to be efficacious, to help facilitate …

Comparison Of Ketamine-Xylazine, Butorphanol-Azaperone-Medetomidine, And Nalbuphine-Medetomidine-Azaperone For Raccoon (Procyon Lotor) Immobilization, Shylo R. Johnson, Christine K. Ellis, Chad Wickham, Molly R. Selleck, Amy T. Gilbert

United States Department of Agriculture Wildlife Services: Staff Publications

Raccoons (Procyon lotor) are frequently handled using chemical immobilization in North America for management and research. In a controlled environment, we compared three drug combinations: ketamine-xylazine (KX), butorphanol-azaperone-medetomidine (BAM), and nalbuphinemedetomidine- azaperone (NalMed-A) for raccoon immobilization. In crossover comparisons, raccoons received a mean of the following: 8.66 mg/kg ketamine and 1.74 mg/kg xylazine (0.104 mL/kg KX); 0.464 mg/kg butorphanol, 0.155 mg/kg azaperone, and 0.185 mg/kg medetomidine (0.017 mL/kg BAM); and 0.800 mg/kg nalbuphine, 0.200 mg/kg azaperone, and 0.200 mg/kg medetomidine (0.020 mL/kg NalMed-A). Induction time was shortest with KX (mean6SE, 10.060.7 min) and longest with NalMed-A (13.061.3 min). …

Using Prescribed Fire And Biosolids Applications As Grassland Management Tools: Do Wildlife Respond?, Brian E. Washburn, Michael J. Begier

United States Department of Agriculture Wildlife Services: Staff Publications

Prescribed burning is a management tool commonly used in forested ecosystems in the southeastern United States, but the influence of this method on grassland vegetation and wildlife in this geographic region is unknown. During 2009–2015, we conducted a study to determine if the application of prescribed burning and/or long-term biosolid applications alter plant communities and/or wildlife use of grassland areas at Marine Corps Air Station Cherry Point, Havelock, North Carolina. We monitored vegetation growth, measured plant community composition, and documented wildlife activity in four study plots for 3 years after the implementation of annual winter prescribed burns. Prescribed burning reduced …

Potential Landscape Connectivity For Invasive Wild Pigs (Sus Scrofa) Across The Northern Prairies Of North America, Corey J. Kramer, Melanie R. Boudreau, Ryan Powers, Kurt C. Vercauteren, Ryan S. Miller, Ryan K. Brook

United States Department of Agriculture Wildlife Services: Staff Publications

Understanding landscape scale connectivity is an essential component in the management of invasive species since connectivity facilitates their invasion potential. Invasive wild pigs (Sus scrofa) are among the most prolific invaders on the planet, causing billions of dollars in agricultural and environmental damage annually. Newly introduced to Canada in the 1980s, we examined wild pig invasion potential across the northern prairies from western Canada into the currently wild pig-free northern U.S. states. We used GPS collar data collected in the Canadian prairies to quantify resource selection and incorporated results into an electric circuit theory framework to evaluate potential …

Rapid Point-Of-Care Testing For Detection Of Antibodies To Toxoplasma Gondii In Black Vultures And Ring-Billed Gulls From Pennsylvania, Alexa Rosypal Von Dohlen, Sydney Randall, Jordyan Grays, Mark A. Dugo, Brian J. Hunt, Justin Brown, Kyle Van Why, Sawsan Ammar, Richard Gerhold

United States Department of Agriculture Wildlife Services: Staff Publications

Toxoplasma gondii is a zoonotic protozoan parasite that infects most warm-blooded animals, including birds. Scavenging birds are epidemiologically important hosts because they can serve as indicators of environmental T. gondii levels. A rapid point-of-care (POC) test that detects antibodies to T. gondii in humans is commercially available. In this research, we assessed the ability of the human POC test to detect anti–T. gondii antibodies in 106 black vultures (Coragyps atratus) and 23 ring-billed gulls (Larus delawarensis) from Pennsylvania, USA. Serum samples were tested with the POC test and compared to the modified …

Low Secondary Risks For Turkey Vultures From A Sodium Nitrite Toxic Bait For Wild Pigs, Justin A. Foster, John C. Kinsey, Michael J. Lavelle, Kurt C. Vercauteren, Nathan P. Snow

United States Department of Agriculture Wildlife Services: Staff Publications

An acute and orally‐delivered toxic bait for wild pigs (Sus scrofa) containing sodium nitrite (SN) is being developed and tested for potential registration in the U.S. We evaluated oral toxicity of SN and risk of secondary consumption in a common avian scavenger, the turkey vulture (Carthartes aura), by 1) conducting an acute oral toxicity test (LD₅₀) for turkey vultures by oral gavage, and 2) allowing turkey vultures to feed on whole carcasses and digestive tracts of wild pigs that died from consuming SN‐laden bait. Captive tests were conducted during fall 2016 and spring 2017 in the …

A Method For Characterizing Disease Emergence Curves From Paired Pathogen Detection And Serology Data, Joshua Hewitt, Grete Wilson-Henjum, Derek T. Collins, Jourdan M. Ringenberg, Christopher A. Quintanal, Robert Pleszewski, Jeffrey C. Chandler, Thomas J. Deliberto, Kim M. Pepin

United States Department of Agriculture Wildlife Services: Staff Publications

1. Wildlife disease surveillance programs and research studies track infection and identify risk factors for wild populations, humans and agriculture. Often, several types of samples are collected from individuals to provide more complete information about an animal's infection history. Methods that jointly analyse multiple data streams to study disease emergence and drivers of infection via epidemiological process models remain underdeveloped. Joint-analysis methods can more thoroughly analyse all available data, more precisely quantifying epidemic processes, outbreak status, and risks.

2. We contribute a paired data modelling approach that analyses multiple samples from individuals. We use ‘characterization maps’ to link paired data …

Torque Teno Sus Virus 1: A Potential Surrogate Pathogen To Study Pig-Transmitted Transboundary Animal Diseases, Xiaolong Li, Brandon M. Parker, Raoul K. Boughton, James C. Beasley, Timothy J. Smyser, James D. Austin, Kim M. Pepin, Ryan S. Miller, Kurt C. Vercauteren, Samantha M. Wisely

United States Department of Agriculture Wildlife Services: Staff Publications

Understanding the epidemiology and transmission dynamics of transboundary animal diseases (TADs) among wild pigs (Sus scrofa) will aid in preventing the introduction or containment of TADs among wild populations. Given the challenges associated with studying TADs in free-ranging populations, a surrogate pathogen system may predict how pathogens may circulate and be maintained within wild free-ranging swine populations, how they may spill over into domestic populations, and how management actions may impact transmission. We assessed the suitability of Torque teno sus virus 1 (TTSuV1) to serve as a surrogate pathogen for molecular epidemiological studies in wild pigs by investigating …

Common Grackle (Quiscalus Quiscula) Predation Of A Great Lakes Piping Plover (Charadrius Melodus) Nest And Its Conservation Implications, Jade R. Arneson, Dennis A. Peloquin, Thomas G. Prestby, Sarah P. Saunders

United States Department of Agriculture Wildlife Services: Staff Publications

Common grackle (Quiscalus quiscula) was observed consuming eggs from an exclosed (protected) Great Lakes piping plover (Charadrius melodus) nest in the lower bay of Green Bay, Lake Michigan in Wisconsin, USA during June 2023. The observation, captured by a trail camera, is the first photographic documentation of grackle predation in any of the three piping plover breeding populations. This behavior adds common grackle to the suite of egg predators and has critical implications for this shorebird species, particularly in the Great Lakes region where piping plover is listed as federally endangered and common grackles are relatively …

Natural Selection On Feralization Genes Contributed To The Invasive Spread Of Wild Pigs Throughout The United States, Niek W. G. Barmentlo, Patrick G. Meirmans, William H. Stiver, Joseph G. Yarkovich, Blake E. Mccann, Antoinette J. Piaggio, Dominic Wright, Timothy J. Smyser, Mirte Bosse

United States Department of Agriculture Wildlife Services: Staff Publications

Despite a long presence in the contiguous United States (US), the distribution of invasive wild pigs (Sus scrofa × domesticus) has expanded rapidly since the 1980s, suggesting a more recent evolutionary shift towards greater invasiveness. Contemporary populations of wild pigs represent exoferal hybrid descendants of domestic pigs and European wild boar, with such hybridization expected to enrich genetic diversity and increase the adaptive potential of populations. Our objective was to characterize how genetic enrichment through hybridization increases the invasiveness of populations by identifying signals of selection and the ancestral origins of selected loci. Our study focused on invasive …

Influence Of Habitat And Baiting Strategy On Oral Rabies Vaccine Bait Uptake By Raccoons In The Southeastern United States, Jacob E. Hill, Madison L. Miller, Richard B. Chipman, Amy T. Gilbert, James C. Beasley, Guha Dharmarajan, Olin E. Rhodea

United States Department of Agriculture Wildlife Services: Staff Publications

terrestrial wildlife species. However, there is currently a limited understanding of how different baiting strategies influence raccoon (Procyon lotor) uptake of ORV baits in non-agricultural habitats in the Southeastern United States, which may hinder the refinement of ORV management for raccoons in this region. Using a combination of new and existing data, we investigated the role of baited area (0.16 vs 3 km²), bait density (75 versus 150 baits/km²) and season (spring versus fall) on placebo ORV bait uptake by raccoons in four rural, non-agricultural habitats in the southeastern United States (bottomland hardwood forest, …