Assessment Of Abdominal Fat Distribution By Computed Tomography In Obese Dogs,
2022
Chulalongkorn University
Assessment Of Abdominal Fat Distribution By Computed Tomography In Obese Dogs, Yunseo Jang, Hyemin Na, Sooyoung Choi, Hojung Choi, Youngwon Lee, Kija Lee
The Thai Journal of Veterinary Medicine
Obesity is a risk factor for canine health but there has been no study on the tendency of body fat accumulation naturally occurring in obese dogs. This retrospective observational study aims to evaluate the tendency to accumulate visceral and subcutaneous fat using computed tomography (CT) in naturally occurring obese dogs. The study included 15 dogs with a body condition score of 8 or 9 that underwent abdominal CT scans. Total fat area (TA), visceral fat area (VA), subcutaneous fat area (SA) and body area (BA) were measured by non-contrast transverse image at the third lumbar vertebra (L3) and the sixth …
Recombinant Scfv Inhibit Newcastle Disease Virus In Vitro Infection,
2022
Chulalongkorn University
Recombinant Scfv Inhibit Newcastle Disease Virus In Vitro Infection, Sanfeng Peng, Lintian Yu, Ying He, Dongyang Chen, Li Wang, Xueliang Zhou, Long Xie, Yangqing Lu
The Thai Journal of Veterinary Medicine
Newcastle disease (ND) is an avian respiratory disease caused by the Newcastle disease virus (NDV) and is prevalent worldwide but lacks a reliable treatment to surmount it. The single-chain fragment variable (scFv) antibody is the smallest functional unit in an immunoglobulin retaining antigen-binding activities. Previous studies have demonstrated that by acting against the NDV phosphoprotein, scFv is capable of neutralizing the virus in infected cells. In this study, two anti-NDV scFv expressing cassettes driven by CAG promoter and EF1α promoter, respectively, were constructed using the piggyBac transposon plasmid backbone. The recombinant plasmids were transfected into DF-1 and 293T cells and …
Genomic And Phylogenetic Analysis Of The Orf7b Gene From A Chinese Feline Infectious Peritonitis Virus Isolate,
2022
Chulalongkorn University
Genomic And Phylogenetic Analysis Of The Orf7b Gene From A Chinese Feline Infectious Peritonitis Virus Isolate, Bo Dong, Bo Dong, Xiaodong Zhang, Xiaodong Zhang, Yafei Song, Chengyu Li, Gaoqiang Zhang, Weiming Lin
The Thai Journal of Veterinary Medicine
FCoV viruses exhibit great genetic diversity, leading to the presence of FIPV-causing variants. Current molecular evolution analysis and genetic variation studies of FCoV in China are predominately focused on gene encoding the spike protein or other structural proteins, while few studies have evaluated genetic variations in nonstructural FCoV genes, which can play an important role in disease pathogenesis. In this study, the gene encoding the open reading frame (ORF) 7b nonstructural FCoV protein of the Chinese Fujian strain FJLY20201 was amplified from the ascitic fluid of a Chinese domestic cat infected with FIPV and compared with ORF 7b from previously …
Detection And Phylogenetic Analysis Of Porcine Epidemic Diarrhea Virus Based On The Orf3 Gene In Pigs In The North Of Vietnam,
2022
Chulalongkorn University
Detection And Phylogenetic Analysis Of Porcine Epidemic Diarrhea Virus Based On The Orf3 Gene In Pigs In The North Of Vietnam, Tran Duc Hoan, Doan Thi Thao, Nguyen Thi Huong Giang, Nguyen Thi Thu Huyen, Nguyen Thi Thu Huyen
The Thai Journal of Veterinary Medicine
To identify further the infectivity, genetic diversity and molecular characteristics of the porcine epidemic diarrhea virus (PEDV). 286 samples of the intestinal tissue of pigs with severe diarrhea were assessed from 5 provinces in the north of Vietnam. The full-length ORF3 gene of 20 representative field strains from 36 farms in 5 provinces was sequenced and analyzed. The prevalence of PEDV was 90.56% and was detected in 259 of 286 samples, with 94.44% (34 of 36) of pig farms harboring the disease. Based on the phylogenetic analysis of the ORF3 genes, our isolates all fell into group G2 (variants) and …
Optimal Infusion Rate Of Hypertonic Saline To Encephalic Vessel Vasodilatation Using Magnetic Resonance Imaging In Normovolemic Dogs,
2022
Chulalongkorn University
Optimal Infusion Rate Of Hypertonic Saline To Encephalic Vessel Vasodilatation Using Magnetic Resonance Imaging In Normovolemic Dogs, Yoshiki Murakami, Kenji Tsukano, Haruyuki Hirata, Hiroshi Koie, Kazuyuki Suzuki
The Thai Journal of Veterinary Medicine
This study aimed to investigate, using magnetic resonance imaging, whether continuous hypertonic saline (HSS) infusion induces a superior vasodilating effect on the encephalic vessels compared to bolus infusion in dogs. The relative cross-sectional area of superior sagittal sinus increased to 1.96±0.09 and 1.69±0.31 times of the pre-value in the continuous and bolus groups, respectively, at t=30 min (p<0.001). However, the relative cross-sectional area of superior sagittal sinus was larger in the continuous group than that in the bolus group (p<0.001). The results suggest that continuous infusion of HSS might be superior to bolus infusion in inducing vasodilatation of the encephalic circulation.
Oxygenated Dressing Quickens Skin Wound Healing Of Bama Miniature Pigs,
2022
Chulalongkorn University
Oxygenated Dressing Quickens Skin Wound Healing Of Bama Miniature Pigs, Yingqiu Wang, Limin Qiao, Hong An, Zhenhao Zhang, Yan Zheng, Chang Chen, Jingrui Zhang, Xiangguo Wang
The Thai Journal of Veterinary Medicine
Oxygen is a vital factor in the process of wound healing and is involved in collagen synthesis, synthesis and the release of growth factors, angiogenesis and epidermal cell contraction. Hence, local oxygen control of wounds plays an important role in skin wound healing. Herein, we developed a novel oxygenated dressing for the treatment of skin wound healing in Bama miniature pigs. The oxygenated dressing was mainly composed of potassium iodide, glucose, glucose oxidase, polyvinyl alcohol, polypropylene, polyvinylpyrrolidone and water. The oxygenated dressing significantly quickens the healing of full-thickness skin wounds in Bama miniature pigs and promotes the proliferation of epidermal …
Stifle Joint Reconstruction Technique Using Fascia Lata In A Cat With Cranial Cruciate Ligament Rupture,
2022
Chulalongkorn University
Stifle Joint Reconstruction Technique Using Fascia Lata In A Cat With Cranial Cruciate Ligament Rupture, Hern-Yeu Lee, Wei-Yau Shia, Tien-Huan Hsu, Wei-Ming Lee
The Thai Journal of Veterinary Medicine
A two-year-old spayed female mongrel cat presenting with right hind limb lameness with intermittent weight bearing while walking was taken to the veterinary medicine teaching hospital of National Chung Hsing University. Both drawer test and tibial compression test were positive, stifle radiography showed synovial fluid had become more radiopaque. The tentative diagnosis for the cat was rupture of the right cranial cruciate ligament (CrCL), medical management using corticosteroids was prescribed with anticoagulants and surgical treatment recommended. Intracapsular reconstruction was performed and the postoperative recovery of this cat presented normal physical status with improved right hind limb weight-bearing activity. This report …
Feline Inductive Odontogenic Tumor In A Young Cat,
2022
Chulalongkorn University
Feline Inductive Odontogenic Tumor In A Young Cat, Chakkarin Satthathum, Sunee Kunakornsawat, Chaiyan Kasorndorkbua
The Thai Journal of Veterinary Medicine
A 6-month-old, 4.45 kg, intact, male domestic shorthair cat was presented with a gingival mass located on the left rostral maxilla and second left upper premolar tooth. Intraoral x-ray of the left rostral maxilla revealed left maxilla bone lysis with a unilocular cystic lesion. The cytology result revealed an epithelial cell tumor .A left rostral maxillectomy was performed and the tumor was submitted for histological assessment .Histopathological diagnosis of the tissue revealed a feline inductive odontogenic tumor .There was no evidence of tumor recurrence 24 months after surgery.
Hepatotoxicity In Acute And Subchronic Oral Gavage Of Ethanolic Extract Of Christia Vespertilionis (L.F.) Bakh. F. Leaf In Male Sprague Dawley Rats,
2022
Chulalongkorn University
Hepatotoxicity In Acute And Subchronic Oral Gavage Of Ethanolic Extract Of Christia Vespertilionis (L.F.) Bakh. F. Leaf In Male Sprague Dawley Rats, Nurul S.A.S., Nurul S.A.S., Hazilawati H., Rosly S.M., Farhan Hanif M.R., Abdullahi A., Shanmugavelu S., Noordin M.M., Mazlina M., Azrina A., Norhaizan M.E.
The Thai Journal of Veterinary Medicine
Increased interest in Christia vespertilionis (L.f.) Bakh. f. leaves is due to their real potential of their health-enhancing properties. This study aims to evaluate the possible in vivo toxicity in 14-day acute and 90-day subchronic oral toxicity of ethanolic extract Christia vespertilionis (L.f.) Bakh. f. in male Sprague Dawley rats. In the acute study, the rats were divided into control, 5% DMSO (vehicle) and 2000 mg/kg groups. For the subchronic toxicity study, a total of 30 rats were divided into five groups consisting of a control, 5% DMSO (vehicle), low dose (75 mg/kg), medium dose (125 mg/kg) and high dose …
Amino Acid Profile Of Rabbit Meat: Dietary Intake And The Effect Of Freezing On The Amino Acid Composition,
2022
Chulalongkorn University
Amino Acid Profile Of Rabbit Meat: Dietary Intake And The Effect Of Freezing On The Amino Acid Composition, Alaa Eldin M.A. Morshdy, Wageh Sobhy Darwish, El Sayed S. El Sebay, El-Shaimaa Mesallam Ali
The Thai Journal of Veterinary Medicine
Rabbit meat is considered as a healthy meat due to its high protein and low fat content. This study aimed at demonstrating the amino acids profile in three rabbit breeds, namely New Zealand White, California and Balady rabbits marketed in Egypt. In addition, the estimated daily intake (EDI) of amino acids due to the consumption of the rabbit meat were calculated. Furthermore, the effect of long frozen storage for different periods on the amino acid content of the New Zealand White rabbit meat was investigated as an experimental trial. The obtained results indicate a slight variation in the amino acid …
Effects Of A Single Dose Hcg Administration On The Induction Of Ovulation, Follicular Dynamics, Uterine Changes And Pregnancy Outcomes In Mares During The Breeding Season,
2022
Chulalongkorn University
Effects Of A Single Dose Hcg Administration On The Induction Of Ovulation, Follicular Dynamics, Uterine Changes And Pregnancy Outcomes In Mares During The Breeding Season, Mesut Çevi̇k, Oğuzhan Ergi̇n, Burcu Esi̇n, Burcu Esi̇n
The Thai Journal of Veterinary Medicine
In this study, 19 mares brought to the clinic at different times with the complaint of not getting pregnant were followed up. For this purpose, changes in the uterine and ovarian dynamics of mares and behavioral changes in estrus were followed and evaluated. In particular, it was attempted to increase the chance and rate of pregnancy by providing ovulation with ovulation follow-up followed by a single dose of hCG administration (Chorulon, 3.000 IU). All manipulations and applications in the study were carried out between March and July, that is, during the breeding season of the mares. During the study, a …
Anesthetic Effects Of Dexmeditomidine-Tiletamine/Zolazepam Combination In Cats Undergoing Ovariohysterectomy,
2022
Chulalongkorn University
Anesthetic Effects Of Dexmeditomidine-Tiletamine/Zolazepam Combination In Cats Undergoing Ovariohysterectomy, Nunnapas Jiwlawat, Cholawat Pacharinsak, Sumit Durongphongtorn
The Thai Journal of Veterinary Medicine
Common injectable anesthetics in cats are alpha-2 receptor agonists and tiletamine/zolazepam. Xylazine shows higher rate of side effects because its lower specificity to alpha-2 receptors than dexmeditomidine. However, the use of drug combinations in cats is not much known. Therefore, alternative anesthetic combination should be investigated. Here, we examined the use of tiletamine/zolazepam with two doses of dexmedetomidine in cats. A total of 24 female cats, weight 3.05 ± 0.10 kg (mean ± SEM). Cats were randomly blinded given intramuscularly injection (IM) with either of such drug combination, representing dosage of low dose dexmedetomidine 7.5 μg/kg with tiletamine/zolazepam 3 mg/kg; …
Serological Diagnostic For Bovine Paratuberculosis In The Municipality Of Sotaquirá, Colombia,
2022
Chulalongkorn University
Serological Diagnostic For Bovine Paratuberculosis In The Municipality Of Sotaquirá, Colombia, Alejandra Buitrago-López, Alejandra Buitrago-López, Alejandra Buitrago-López, Diana M. Bulla-Castañeda, Adriana M. Díaz-Anaya, Diego J. Garcia-Corredor, Julio Tobón Torreglosa, Diego Ortiz Ortega, Martín O. Pulido-Medellín
The Thai Journal of Veterinary Medicine
Bovine Paratuberculosis (BPT) or Johne’s disease is a pathology caused by Mycobacterium avium subsp. paratuberculosis (MAP). The parasite causes chronic granulomatous enteritis in domestic and wild grazers. Oral-fecal transmission is the most common form of transmission with calves less than six months old being the most susceptible but not presenting clinical symptoms until five years later. It is a disease that spreads during dairy production generating economic loss due to low productive yield and the early discarding of infected animals. The objective of our study was to perform a serological diagnostic of BPT in herds from the municipality of Sotaquirá, …
Complementary Effects Of White Rose Petal Extract On Canine Atopic Dermatitis,
2022
Chulalongkorn University
Complementary Effects Of White Rose Petal Extract On Canine Atopic Dermatitis, Seo-Jin Park, Taesik Yun, Yoonhoi Koo, Yeon Chae, Dohee Lee, Jooyoung Park, Yujin Choi, Ehn-Kyoung Choi, Hakhyun Kim, Yun-Bae Kim, Mhan-Pyo Yang, Byeong-Teck Kang
The Thai Journal of Veterinary Medicine
This study aimed to investigate the therapeutic efficacy of white rose petal extract (WRPE) in canine atopic dermatitis (CAD). Experiment 1 involved clinical assessments using atopic dogs and experiment 2 used beagles with experimentally induced dermatitis. The atopic dogs were divided into four groups: 1% WRPE solution (group 1), 0.5% WRPE solution (group 2), 1% WRPE cream (group 3) and 1% WRPE cream containing lipid granules (group 4). Three normal beagles were used for the experimental induction of dermatitis. Experimental dermatitis was induced by intradermal injections of histamine dihydrochloride, compound 48/80 or substance P. WRPE solution or cream was applied …
Occurrence And Characteristics Of Salmonella Isolated From Various Vegetable Sources: Potential For The Human-Food Interface In Salmonellosis In Vientiane, The Capital Of Laos Pdr,
2022
Chulalongkorn University
Occurrence And Characteristics Of Salmonella Isolated From Various Vegetable Sources: Potential For The Human-Food Interface In Salmonellosis In Vientiane, The Capital Of Laos Pdr, Dethaloun Meunsene, Thanaporn Eiamsam-Ang, Prapas Patchanee, Ratchadaporn Boripun, Phacharaporn Tadee, Sayaphet Rattanavong, Manivanh Vongsouvath, Pakpoom Tadee
The Thai Journal of Veterinary Medicine
Salmonella is one of the most frequently reported causes of bacterial foodborne outbreaks in the world. Despite this, cases associated with raw vegetable consumption have increased, however very few studies have focused on this. The present study is an overview of the role of vegetables as a concern for Salmonella circulating in Vientiane, the capital of the Lao People's Democratic Republic (Lao PDR). A cross-sectional study was surveyed during the first half 2019 on various types of vegetable sold in retailers in the area. The prevalence, serotype, antimicrobial resistance pattern and genetic profile of Salmonella were investigated. In this study, …
Yield, Composition, Fatty Acid Profile And Cla Content Of Milk From Goats Fed With Different Levels Of Opf,
2022
Chulalongkorn University
Yield, Composition, Fatty Acid Profile And Cla Content Of Milk From Goats Fed With Different Levels Of Opf, Pitunart Noosen, Ulia Renfelia Baysi
The Thai Journal of Veterinary Medicine
The objective of this study was to evaluate the effect of different inclusion levels of OPF in total mixed ration (TMR)-based diet on yield, composition, FA profile and CLA content of milk in dairy goats. Six nulliparous female dairy goats (Saanen x Thai native, 19-21 months old, first lactation) were randomly set according to a 3x3 Latin square design with 2 replications for receiving diets consisted of 0% OPF (OPF0), 20% OPF (OPF20) and 40% OPF (OPF40). The average weight of the goats was 32.48±1.30 kg (mean±s.d.). The findings of this study showed that dry matter intake (DMI) and all …
Inconsistent And Multiple Bacterial Species From Different Sample Types Of Dogs With Urolithiasis And Bacterial Cystitis,
2022
Chulalongkorn University
Inconsistent And Multiple Bacterial Species From Different Sample Types Of Dogs With Urolithiasis And Bacterial Cystitis, Nantapatt Pridsadangbud, Chantamanee Chantarasiri, Nattanit Visitkitjakarn, Phitsinee Tantivimonkajor, Jitrapa Yindee, Nicole Sirisopit Mehl
The Thai Journal of Veterinary Medicine
Urinary tract infection is the most common complication found in dogs with urolithiasis. Bacterial cystitis can play an important role in dogs with urolithiasis; it can either be the cause of cystic formation or be a complication following urolithiasis. Moreover, urolithiasis cases that come with bacterial cystitis can lead to a condition of complicated cystitis which will require more complicated treatment and management. Proper bacterial identification should be performed in order to follow an appropriate treatment. Specific treatments require bacterial identification in order to select the most appropriate antibiotics. There are three main possible sites for bacterial sample collection in …
Distribution Of Salmonella Spp. And Serovars Isolated From Captive Reptiles In Four Zoos Of The Zoological Park Organization, Thailand, Between 2017 And 2018,
2022
Chulalongkorn University
Distribution Of Salmonella Spp. And Serovars Isolated From Captive Reptiles In Four Zoos Of The Zoological Park Organization, Thailand, Between 2017 And 2018, Sompoth Weerakhun, Paweena Kongsanan, Wichit Kongkham
The Thai Journal of Veterinary Medicine
Salmonellosis is the main reason for food borne diseases in many countries globally and it has been found that reptiles are carriers of the disease. Therefore, this study was performed to determine the distribution of Salmonella spp. and their serovars from reptiles in zoos in Thailand between 2017 and 2018. A total of 257 reptile samples were collected from four zoos which are Dusit (128 samples), Chiangmai (32 samples), Songkhla (34 samples) and Nakhonratchasima (63 samples). Salmonella was isolated and identified, serotyping of those isolates was investigated using a panel of Salmonella antisera and serotypes were characterized according to the …
Investigation Of The Microstructure And Mechanical Properties Of The Domestic Cat’S Paw Pads,
2022
Chulalongkorn University
Investigation Of The Microstructure And Mechanical Properties Of The Domestic Cat’S Paw Pads, Ye Mei
The Thai Journal of Veterinary Medicine
Domestic cats have a biological function such as cushioning when they are jumping or walking which is mainly due to the biological coupling system formed in the long-term evolution process of nature, including the trunk-leg-paw pad structure. Among them, the paw pad, as the only part of the body that directly contacts with the ground in the process of movement, plays a key role in its biological function. Hence, in order to clarify the micro structure, mechanical properties and functional characteristics of the paw pad and reveal the relationship between it and the domestic cat’s biological function, this paper has …
Immunochromatographic Test (Ict) For The Detection Of Antibody Against Alv-J,
2022
Chulalongkorn University
Immunochromatographic Test (Ict) For The Detection Of Antibody Against Alv-J, Guihua Wang, Yanming Wang, Ziqiang Cheng
The Thai Journal of Veterinary Medicine
ALV-J is an important pathogen in the poultry industry in China. In this study, we developed an immunochromatographic test (ICT) assay for the detection of antibody against of ALV-J to evaluate ALV-J infection. Gp85 protein of ALV-J was employed as a capture antigen. Antigen-antibody reaction was detected by either colloidal-gold conjugated gp85 protein or monoclonal antibody of ALV-J. The high consistency of results in ICT and ELISA indicated that the ICT is reliable. This suggests that the gp85-based ICT can be used as a rapid on-site diagnostic method of ALV-J.
