Radiographic Cardiac Indices For The Evaluation Of Cardiac And Left Atrial Sizes In Healthy Wistar Albino Rats (Rattus Norvegicus),
2022
Chulalongkorn University
Radiographic Cardiac Indices For The Evaluation Of Cardiac And Left Atrial Sizes In Healthy Wistar Albino Rats (Rattus Norvegicus), Mehmet Alper Çeti̇nkaya, Mahir Kaya
The Thai Journal of Veterinary Medicine
This study was performed for the purpose of comparing vertebral heart score (VHS), radiographic left atrial dimension (RLAD) and vertebral left atrial size (VLAS) values derived from plain and contrast right lateral thoracic radiographs from 70 healthy, young adult Wistar albino rats and to identify reference values for these radiographic cardiac indices. Measurements were taken by two observers blinded to the study data. VHS, RLAD and VLAS median values and reference ranges were 8v and 7-9.3v for plain radiography, 8.2v and 7.4-9.5v for contrast radiography (P< 0.000), 1.3v and 1-1.8v for plain radiography, 1.3v and 1.1-1.6v for contrast radiography (P <0.000) and 1.9v and 1.4-2.4v for plain radiography and 1.95v 1.5-2.4v for contrast radiography (P<0.001), respectively. The only statistically significant difference was observed in terms of gender between VHS values from plain and contrast radiographs (P<0.02). Intraobserver and interobserver measurements revealed excellent agreement for radiographic cardiac indices obtained from contrast radiography. The study measurements indicate that contrast radiography provides a more effective quantitative assessment of cardiac and left atrial sizes than plain radiography. The radiographic cardiac index measurements presented in this study can serve as a reference baseline for both veterinarians and experimental cardiovascular studies.
Haplotype Variation Of Partial Sry Gene In Ongole Grade Bulls (Bos Indicus) Of Indonesia,
2022
Chulalongkorn University
Haplotype Variation Of Partial Sry Gene In Ongole Grade Bulls (Bos Indicus) Of Indonesia, Widya Pintaka Bayu Putra, Saiful Anwar, Slamet Diah Volkandari, Syahruddin Said
The Thai Journal of Veterinary Medicine
Ongole grade cattle (Bos indicus) are Indonesian beef cattle that adapt well in a tropical climate. This study was aimed to observe the genetic diversity of partial Sex-determining of the region Y chromosome (SRY) gene (625 bp) in Ongole grade bulls (Bos indicus) of Indonesia. A total twenty-three (23) DNA samples of animal studies were collected from frozen sperm (straws) produced by the National Artificial Insemination Centers (NAICs) of Lembang (13 straws) and Singosari (10 straws). Research showed that a total of four (4) insertion mutations were detected in the intronic region with the position of 1831th, 1844th, 1850th and …
Computed Tomographic Findings Of Primary Renal Tumors In Dogs And Cats,
2022
Chulalongkorn University
Computed Tomographic Findings Of Primary Renal Tumors In Dogs And Cats, Daji Noh, Jaejin Shim, Sooyoung Choi, Hojung Choi, Youngwon Lee, Kija Lee
The Thai Journal of Veterinary Medicine
Primary renal tumor is uncommon in dogs and cats, with few previous computed tomography (CT) reports. Previous studies have reported that contrast-enhancing CT is helpful in differentiating the type of renal tumors. This study aimed to describe CT findings of renal tumors in dogs and cats and identify contrast-enhancing pattern according to 3 post-contrast phases. In this retrospective study, the following CT findings were recorded for each patient: (1) renal tumor involvement, (2) enhancement pattern, (3) vascular invasion, (4) presence of lymphadenopathy and organ metastasis, (5) presence of mineralization, and (6) attenuation values of renal tumors on pre- and post-contrast …
Ovarian Stimulation With A Low Dose Of Follicle Stimulating Hormone Improved Recovery Rates And Developmental Competence Of Goat Oocytes,
2022
Chulalongkorn University
Ovarian Stimulation With A Low Dose Of Follicle Stimulating Hormone Improved Recovery Rates And Developmental Competence Of Goat Oocytes, Pintira Thiangthientham, Nitira Anakkul, Junpen Suwimonteerabutr, Wirakan Kallayanathum, Sawita Santiviparat, Mongkol Techakumphu, Theerawat Tharasanit
The Thai Journal of Veterinary Medicine
The aim of this study was to optimise the ovarian responses using different FSH (follicle stimulating hormone) treatments in goats. Group 1 (control), the ovaries were stimulated with a total dose of 200 mg FSH per doe. The FSH injections were performed at 12-h intervals starting on day 9 after progesterone implantation. The FSH doses were decreased to 160 mg/doe and 130 mg/doe for Groups 2 and 3, respectively. The numbers of small, medium and large follicles were determined by real-time B mode ultrasonography. Ovum pick-up (OPU) was performed by laparoscopy under general anesthesia at 96, 42 and 36 h …
Surgical Removal Of Urinary Bladder Urolith In Female Asian Elephant (Elephas Maximus) By Episiotomy And Urinary Bladder Sphincterotomy,
2022
Chulalongkorn University
Surgical Removal Of Urinary Bladder Urolith In Female Asian Elephant (Elephas Maximus) By Episiotomy And Urinary Bladder Sphincterotomy, Nikorn Thongtip, Supaphen Sripiboon, Nattaphol Muangthong, Suteeranun Pipitwanichtham, Smit Srisomrun, Patcharida Dittawong, Phawaran Meetipkit, Ratiwan Sitdhibutr, Warangkhana Lankaphin, Petthisak Sombutputorn, Weerapongse Tangjitjaroen
The Thai Journal of Veterinary Medicine
A 50-year-old female Asian elephant (Elephas maximus) was referred to the Kasetsart University Veterinary Teaching Hospital with histories of anuria and abdominal pain for five days. Trans-rectal ultrasonography demonstrated the presence of hyperechogenic uroliths located between the neck and trigone of the urinary bladder. Vestibuloscopy by endoscope revealed an abnormal brownish mass obstructing the internal bladder orifice. Episiotomy was performed after standing sedation with dexmedetomidine, epidural anesthesia and perineal infiltration with lidocaine. A single 1.7 kg urinary bladder urolith with 17x12x10 cm dimension was manually removed using a combination of several methods, including sphincter dilatation using lidocaine ring infiltration at …
Microbial Diversity And Microbiological Quality Of Edible-Birdnest (Ebn) During Processing And Storage,
2022
Chulalongkorn University
Microbial Diversity And Microbiological Quality Of Edible-Birdnest (Ebn) During Processing And Storage, Shazreena Zuber, Zunita Zakaria, Jalila Abu, Saleha Abdul Aziz
The Thai Journal of Veterinary Medicine
The microbial quality of 45 EBN consisting of raw unclean and raw clean Edible-birdnest (EBN) from two different premises were analyzed for 8 weeks. Using 3M™ Petrifilm, Total Aerobic Count (TAC) of raw clean EBN and Total Coliform Count (TCC) for raw unclean, 3 days, 2 weeks and 4 weeks EBN in both premises exceeded the limit stated in by the Malaysian Standard (MS 2334:2011; SIRIM/DVS 3:2015). A total of 264 bacteria and 208 fungi species were isolated. Bacteria isolated was identified using bioMérieux’s API® kit and a series of biochemical tests. Fungi were identified using techniques described by the …
Odor Detection Of Cancer Cell Metabolites By Scent-Detection Dogs,
2022
Chulalongkorn University
Odor Detection Of Cancer Cell Metabolites By Scent-Detection Dogs, Geontae Park, Dongwook Kim, Seongsoo Jeong, Dongsun Park, Gonhyung Kim
The Thai Journal of Veterinary Medicine
Dogs have a superior olfactory system; thus, they have been trained and used to detect various nonbiological and biological scents. In addition, many studies have recently reported dogs’ ability to detect the odor of certain cancers or cancer cells. A previous study documented that a dog trained to detect a certain malignant cancer cell could also detect another, unfamiliar malignant cancer cell well, implying that these two cancer cells share a certain specific odor. Thus, given the hypothesis that malignant cancer cells of different origins may contain a common cancer-specific odor, the purpose of the present study was to evaluate …
A Study Of Standard Values Of Two-Dimensional, M-Mode And Doppler Echocardiography In Conscious Small Domestic Rabbits,
2022
Chulalongkorn University
A Study Of Standard Values Of Two-Dimensional, M-Mode And Doppler Echocardiography In Conscious Small Domestic Rabbits, Nattanicha Ngosurachet, Tipparat Tangtraitha, Preeyanit Kaewyara, Pattharat Asvaesoontorn, Thawat Lekdumrongsak, Sirilak Disatian Surachetpong
The Thai Journal of Veterinary Medicine
To determine normal cardiac values for two-dimensional, M-mode and Doppler echocardiography because standard echocardiographic values have not been adequately studied in small-breed rabbits. Data was collected from 40 healthy small-breed rabbits. The results of complete physical examination, thoracic radiography, electrocardiography and echocardiography were collected and analysed as descriptive statistics. The correlation between weight and age and echocardiographic parameters was evaluated. A t-test was performed to compare the parameters between males and females. Most parameters were normally distributed and could be reliably used as standard values of echocardiographic parameters. Weight and age correlated positively with several echocardiographic parameters. However, there was …
Comparison Of Biomechanical Properties Associated With The Diameter And Insertion Depth Of Bone Anchors In The Femoral Condyle Of Toy Breed Cadaver Dogs,
2022
Chulalongkorn University
Comparison Of Biomechanical Properties Associated With The Diameter And Insertion Depth Of Bone Anchors In The Femoral Condyle Of Toy Breed Cadaver Dogs, Yun-Hee An, Mu-Young Kim, Hun-Young Yoon
The Thai Journal of Veterinary Medicine
The objective of the present study was to compare the biomechanical properties of implanted bone anchors based on diameter and insertion depth to yield selection criteria for bone anchor size in toy breed dogs. Twenty toy breed dog cadavers weighing < 5 kg underwent placement of five types of veterinary bone anchors (short diameter, short length [SDSL]; short diameter, medium length [SDML]; medium diameter, medium length [MDML]; medium diameter, long length [MDLL]; and long diameter, medium length [LDML]) at a predetermined femoral attachment site. Anchor screw depth/femoral condyle width (FCW) and anchor screw diameter/femoral condyle length (FCL) were measured using radiography. The yield load, Young’s modulus and failure load were measured and the causes of failure for each construct were recorded. The anchor screw depth/FCW was < 50%, 50%–75%, ~50%, and 75%–100% in the SDSL, SDML, MDML, and MDLL groups, respectively. The yield load, Young’s modulus and failure load were higher in the SDML and MDLL groups than in the SDSL and MDML groups. The anchor screw diameter/FCL was 12%–15% and 24%–30% in the SDML and LDML groups, respectively. No differences in biomechanical parameters were found between the SDML and LDML groups. The cause of failure in all constructs was pull-out of the bone anchor, except for distal femur fracture in five LDML constructs. In conclusion, when implanting bone anchors in toy breed dogs, the insertion depth should be > 50% of the FCW, regardless of diameter. Moreover, distal femur fracture can occur if the bone anchor diameter/FCL ratio exceeds 24%.
Environmental Enrichment And The Behavioral Effects On Captive Beluga Whales (Delphinapterus Leucas),
2022
Chulalongkorn University
Environmental Enrichment And The Behavioral Effects On Captive Beluga Whales (Delphinapterus Leucas), Tsung-Hsien Li, Evonne Lim, I-Chun Chen, Chuen-Fu Lin, Shih-Chu Chen, Ming-An Tsai
The Thai Journal of Veterinary Medicine
Environmental enrichment methods are meant to encourage animals to display their complete ethogram while under human care in zoos and aquariums. In this study, we sought to characterize the effectiveness of environmental enrichment (involving the inclusion of toys in the living environment) on captive beluga whales in the National Museum of Marine Biology and Aquarium. Our results indicate that belugas with toys spend less time floating and swimming repetitively than do belugas without toys. The presence of toys was also shown to enhance social interactions among captive belugas. Overall, our findings indicate that the provision of toys can enrich the …
Molecular Determination Of West Nile Virus, Equine Herpesvirus 1 And Herpesvirus 4 In Feral Horses In Turkey,
2022
Chulalongkorn University
Molecular Determination Of West Nile Virus, Equine Herpesvirus 1 And Herpesvirus 4 In Feral Horses In Turkey, Irmak Dik, Oguzhan Avci, Sibel Yavru, H. Pelin Aslim, H.Sercan Palanci
The Thai Journal of Veterinary Medicine
West Nile Virus (WNV), Equine Herpesvirus 1 (EHV-1) and Equine Herpesvirus 4 (EHV-4) are among the important pathogenic viral factors for both domestic and feral horses. This study was carried out to determine the molecular presence of WNV, EHV-1 and EHV-4 infections in feral horses in Konya. Blood samples were taken from 36 horses selected by a randomized sampling method from a total of 250 feral horses bred together. The leukocyte samples obtained were examined virologically for the presence nucleic acid of WNV using the Nested reverse transcriptase-polymerase chain reaction assay (nested RT-PCR) method and using molecular methods for the …
Cloning, Expression And Identification Of Truncated Spike Gene Of Ibv (Sf200) And Chicken Granulocyte- Macrophage Colony Stimulating Factor (Gm-Csf),
2022
Chulalongkorn University
Cloning, Expression And Identification Of Truncated Spike Gene Of Ibv (Sf200) And Chicken Granulocyte- Macrophage Colony Stimulating Factor (Gm-Csf), Tran Duc Hoan, Doan Thi Thao, Wang Bin
The Thai Journal of Veterinary Medicine
The aim of this study was to clone, express and identify the truncated S1 gene of nephrotropic infectious bronchitis virus (IBV) and granulocyte-monocyte colony stimulating factor (GM-CSF) of chicken. Firstly, two genes were amplified by polymerase chain reaction (PCR) and cloned into pMD18-T vector. The truncated S1 gene designated as Sf200 (containing five antigenic sites of S1 glycoprotein on amino acid residues (aa) 24–61, (aa) 291–398 and (aa) 497–543 and GM-CSF were then amplified from the respective recombinant pMD18-T plasmids and cloned into pET-32a (+) vector resulting pET-Sf200, pET-GM which were identified by restriction enzyme digestion and sequencing analysis. The …
Efficacy Of Immune Complex Infectious Bursal Disease Vaccines Against Very Virulent Infectious Bursal Disease Virus Challenge In Commercial Layer Chicken,
2022
Chulalongkorn University
Efficacy Of Immune Complex Infectious Bursal Disease Vaccines Against Very Virulent Infectious Bursal Disease Virus Challenge In Commercial Layer Chicken, Nataya Charoenvisal, Phornphisut Areeraksakul, Choew Kong Mah, Nida Sirikobkul, Nida Sirikobkul
The Thai Journal of Veterinary Medicine
The vaccination program for controlling Infectious bursal disease (IBD) was complicated due to high maternal antibody in day old chick. Therefore, our study was performed in young layer chickens with high maternal antibody as commonly seen in our country. The objective of this study was to determine the protective efficacy of immune complex IBD vaccines against very virulent infectious bursal disease virus (vvIBDV) challenge in commercial layer chickens. As immune complex vaccine A was recommended in layers by the company, the first aim of the study was to compare immune complex vaccine A (group 1) and B (group 3). The …
Molecular Detection And Isolation Of Mycoplasma Conjunctivae From Goats With Infectious Keratoconjunctivitis In Sichuan Province, Southwest China,
2022
Chulalongkorn University
Molecular Detection And Isolation Of Mycoplasma Conjunctivae From Goats With Infectious Keratoconjunctivitis In Sichuan Province, Southwest China, Youwen Yang, Yanmin Li, Xiaofang Dao, Lei Fei, Falong Yang
The Thai Journal of Veterinary Medicine
Infectious keratoconjunctivitis (IKC) is a common contagious ocular disease in ruminants worldwide characterized with keratitis and conjunctivitis and, in severe cases, blindness. Recently two outbreaks of infectious keratoconjunctivitis (IKC) occurred in goats in Sichuan Province, China. To identify the causative agent responsible for these outbreaks, ocular swabs were then taken from 40 goats with IKC and 22 goats without IKC for isolation and molecular detection of mycoplasma. Mycoplasma conjunctivae-specific nested-PCR was 85.0% positive among samples from goats with IKC and 40.9% positive from goats without IKC. Nine PCR products were sequenced and showed that there was 99.6-100% sequence homology among …
Enterococcus Faecium Septicemia In A Dog: Clinical Features And Molecular Screening Of Virulence Genes Of Enterococcus,
2022
Chulalongkorn University
Enterococcus Faecium Septicemia In A Dog: Clinical Features And Molecular Screening Of Virulence Genes Of Enterococcus, Trasida Ployngam, Numfa Fungbun
The Thai Journal of Veterinary Medicine
A one-year-old Golden Retriever male dog manifested multi-systemic inflammation with a marked increment of plasma D-dimer. Enterococcus spp. was identified from hemoculture and it showed multidrug resistance (MDR), including amikacin, cephazolin, ceftriaxone, clindamycin, imipenem, penicillin G and sulfa-trimethoprim. Pure Enterococcus colonies were identified for species-specific DNA by PCR amplification and sequencing. It was 100% identical with the Enterococcus faecium strains in the National Center for Biotechnology Information (NCBI) database. Molecular screening of virulence genes of the isolated E. faecium strain exhibited four of nine genes including gelatinase (gelE), cell wall adhesion (efaAfm), second collagen adhesin (scm) and adhesin of collagen …
Acquired Indirect Unilateral Chronic Reducible Scrotal Hernia In An Arabian Stallion,
2022
Chulalongkorn University
Acquired Indirect Unilateral Chronic Reducible Scrotal Hernia In An Arabian Stallion, Mohamed A. Marzok, Adel I. Almubarak, Mahmoud Kandeel, Adel A. Badawy
The Thai Journal of Veterinary Medicine
This case report describes the presentation and surgical management of acquired indirect unilateral chronic reducible scrotal herniation in an 8-year-old Arabian stallion. The horse had a history of moderate unilateral swelling of the scrotum that had been noticed by the owner for 10 months. Upon palpation, the swelling was soft, reducible, painless and not accompanied by an acute abdomen. Ultrasonography, rectal and clinical examination were useful tools for the diagnosis of scrotal hernias in this case. A successful surgical herniorrhaphy was performed with the removal of the testicle at the affected side and the superficial inguinal ring was closed by …
Therapeutic Effects Of Sildenafil Combined With Low-Dosage Imatinib On Pulmonary Hypertension In Five Dogs,
2022
Chulalongkorn University
Therapeutic Effects Of Sildenafil Combined With Low-Dosage Imatinib On Pulmonary Hypertension In Five Dogs, Yu Sahashi, Yoshiaki Hikasa
The Thai Journal of Veterinary Medicine
Five dogs were diagnosed with pulmonary hypertension (PH) secondary to chronic degenerative mitral valve disease or ventricular septal defect. They were initially administered low-dose imatinib mesylate (3 mg/kg, q24h) without changes to the background therapy to treat PH. The low-dose imatinib improved the right ventricular (RV) function parameters including estimated systolic and mean pulmonary arterial pressures (sPA and mPAP) and clinical symptoms. On day 53 to day 168 after imatinib administration, as PH and clinical signs worsened, low-dose sildenafil (0.5 mg/kg, q12h) was administered in combination with imatinib. This combination greatly reduced the estimated sPA and mPAP, and improved clinical …
Divergent Neural And Endocrine Responses In Wild-Caught And Laboratory-Bred Rattus Norvegicus,
2022
University of Richmond
Divergent Neural And Endocrine Responses In Wild-Caught And Laboratory-Bred Rattus Norvegicus, Joanna Jacob, Sally Watanabe, Jonathan Richardson, Nick Gonzales, Emily Ploppert, Garet Lahvis, Aaron Shiels, Sadie Wenger, Kelly Saverino, Janhavi Bhalerao, Brendan Crockett, Erin Burns, Olivia Harding, Krista Fischer-Stenger, Kelly Lambert
United States Department of Agriculture Wildlife Services: Staff Publications
Although rodents have represented the most intensely studied animals in neurobiological investigations for more than a century, few studies have systematically compared neural and endocrine differences between wild rodents in their natural habitats and laboratory strains raised in traditional laboratory environments. In the current study, male and female Rattus norvegicus rats were trapped in an urban setting and compared to weight-and sex-matched conspecifics living in standard laboratory housing conditions. Brains were extracted for neural assessments and fecal boli were collected for endocrine [corticosterone and dehydroepiandrosterone (DHEA)] assays. Additionally, given their role in immune and stress functions, spleen and adrenal weights …
Clinical Ophthalmic Parameters Of The Quaker Parrot (Myiopsitta Monachus),
2022
Lincoln Memorial University
Clinical Ophthalmic Parameters Of The Quaker Parrot (Myiopsitta Monachus), Emily Halsmer, Jennifer Jill Heatley Ms, Dvm, Dabvp (Avian, Reptilian, Amphibian), Daczm, Erin M. Scott Dvm, Dacvo
Research Day
Purpose: Ophthalmic diagnosis in many avian species remains hindered by lack of normative values. This study aimed to establish normal ophthalmic parameters for select diagnostic tests in clinically normal Quaker parrots. Methods: Ninety-six captive Quaker parrots ages 8-18 years underwent ophthalmic examination to include assessment of neuro-ophthalmic reflexes, phenol red thread test, rebound tonometry, fluorescein staining, palpebral fissure length measurements, slit lamp biomicroscopy, indirect fundoscopy, and ocular ultrasound biometry. Results: Menace response, dazzle reflex, and direct pupillary light reflex were present for all Quaker parrots. Tear production (mean ± SD) was 13.3 ± 4.0 mm/15 sec and intraocular pressure (IOP, …
Experimental Infection Of Brazilian Free-Tailed Bats (Tadarida
Brasiliensis) With Two Strains Of Sars-Cov-2,
2022
Colorado State University
Experimental Infection Of Brazilian Free-Tailed Bats (Tadarida Brasiliensis) With Two Strains Of Sars-Cov-2, Angela M. Bosco-Lauth, Stephanie M. Porter, Karen A. Fox, Mary E. Wood, Daniel Neubaum, Marissa Quilici
United States Department of Agriculture Wildlife Services: Staff Publications
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is presumed to have originated from wildlife and shares homology with other bat coronaviruses. Determining the susceptibility of North American bat species to SARS-CoV-2 is of utmost importance for making decisions regarding wildlife management, public health, and conservation. In this study, Brazilian free-tailed bats (Tadarida brasiliensis) were experimentally infected with two strains of SARS-CoV-2 (parental WA01 and Delta variant), evaluated for clinical disease, sampled for viral shedding and antibody production, and analyzed for pathology. None of the bats (n = 18) developed clinical disease associated with infection, shed infectious virus, or …
