Development Of A Fluorescent Microsphere Immunoassay For Detection Of Antibodies Against Porcine Reproductive And Respiratory Syndrome Virus Using Oral Fluid Samples As An Alternative To Serum-Based Assays,
2012
South Dakota State University
Development Of A Fluorescent Microsphere Immunoassay For Detection Of Antibodies Against Porcine Reproductive And Respiratory Syndrome Virus Using Oral Fluid Samples As An Alternative To Serum-Based Assays, Robert J. Langenhorst, Steven Lawson, Apisit Kittawornrat, Jeffery J. Zimmerman, Zhi Sun, Yanhua Li, Jane Christopher-Hennings, Eric A. Nelson, Ying Fang
Veterinary and Biomedical Sciences Faculty Publications
For effective disease surveillance, rapid and sensitive assays are needed to detect antibodies developed in response to porcine reproductive and respiratory syndrome virus (PRRSV) infection. In this study, we developed a multiplexed fluorescent microsphere immunoassay (FMIA) for detection of PRRSV-specific antibodies in oral fluid and serum samples. Recombinant nucleocapsid protein (N) and nonstructural protein 7 (nsp7) from both PRRSV genotypes (type I and type II) were used as antigens and covalently coupled to Luminex fluorescent microspheres. Based on an evaluation of 488 oral fluid samples with known serostatus, the oral fluid-based FMIAs achieved >92% sensitivity and 91% specificity. For serum …
Method For Screening For A Tobiano Coat Color Genotype,
2012
University of Kentucky
Method For Screening For A Tobiano Coat Color Genotype, Ernest Bailey, Samantha A. Brooks
Veterinary Science Faculty Patents
A method for screening for a Tobiano genotype includes obtaining a nucleic acid from an equine animal, and analyzing the nucleic acid for the presence of an inversion in a chromosome ECA3q which is indicative of the genotype for Tobiano. The method includes detecting at least one of a telomeric breakpoint of an inverted ECA3q chromosome and/or a centromeric breakpoint of an inverted ECA3q chromosome. In one embodiment, the nucleic acid may be analyzed by the steps of hybridizing the group of probes or primers having the sequences set forth herein in SEQ ID NO:8, SEQ ID NO: 9, and …
Zwitterionic Chitosan Derivative, A New Biocompatible Pharmaceutical Excipient, Prevents Endotoxin-Mediated Cytokine Release,
2012
Purdue University
Zwitterionic Chitosan Derivative, A New Biocompatible Pharmaceutical Excipient, Prevents Endotoxin-Mediated Cytokine Release, Gaurav Bajaj, William G. Van Alstine, Yoon Yeo
Department of Comparative Pathobiology Faculty Publications
Chitosan is a cationic polymer of natural origin and has been widely explored as a pharmaceutical excipient for a broad range of biomedical applications. While generally considered safe and biocompatible, chitosan has the ability to induce inflammatory reactions, which varies with the physical and chemical properties. We hypothesized that the previously reported zwitterionic chitosan (ZWC) derivative had relatively low pro-inflammatory potential because of the aqueous solubility and reduced amine content. To test this, we compared various chitosans with different aqueous solubilities or primary amine contents with respect to the intraperitoneal (IP) biocompatibility and the propensity to induce proinflammatory cytokine production …
A High Density Snp Array For The Domestic Horse And Extant Perissodactyla: Utility For Association Mapping, Genetic Diversity, And Phylogeny Studies,
2012
University of Minnesota - Twin Cities
A High Density Snp Array For The Domestic Horse And Extant Perissodactyla: Utility For Association Mapping, Genetic Diversity, And Phylogeny Studies, Molly E. Mccue, Danika L. Bannasch, Jessica L. Petersen, Jessica Gurr, Ernie Bailey, Matthew M. Binns, Ottmar Distl, Gérard Guérin, Telhisa Hasegawa, Emmeline W. Hill, Tosso Leeb, Gabriella Lindgren, M Cecilia T. Penedo, Knut H. Røed, Oliver A. Ryder, June E. Swinburne, Teruaki Tozaki, Stephanie J. Valberg, Mark Vaudin, Kerstin Lindblad-Toh, Claire M. Wade, James R. Mickelson
Veterinary Science Faculty Publications
An equine SNP genotyping array was developed and evaluated on a panel of samples representing 14 domestic horse breeds and 18 evolutionarily related species. More than 54,000 polymorphic SNPs provided an average inter-SNP spacing of ∼43 kb. The mean minor allele frequency across domestic horse breeds was 0.23, and the number of polymorphic SNPs within breeds ranged from 43,287 to 52,085. Genome-wide linkage disequilibrium (LD) in most breeds declined rapidly over the first 50-100 kb and reached background levels within 1-2 Mb. The extent of LD and the level of inbreeding were highest in the Thoroughbred and lowest in the …
Investigation Of The Effects Of Co2 Insufflation On Blood Gas Values During Laparoscopic Procedures In Pigs,
2012
TÜBİTAK
Investigation Of The Effects Of Co2 Insufflation On Blood Gas Values During Laparoscopic Procedures In Pigs, Özlem Güzel, Di̇lek Olğun Erdi̇kmen, Di̇dar Aydin, Zi̇hni̇ Mutlu, Esma Yildar
Turkish Journal of Veterinary & Animal Sciences
Due to the fact that they produce less post-operative pain and shorter hospitalisation times, laparoscopic surgery procedures are more widely used than many traditional open surgical methods. However, the carbon dioxide gas used to visualise the surgical site during the procedure causes complications such as an increase in the blood CO_2 level and acidosis. In the present study, 12 male pigs were used. Sedation was achieved via intramuscular injection of xylazine at a dose of 2 mg/kg and anaesthesia induction was done via slow intravenous injection of ketamine HCl at a dose of 2 mg/kg. Following endotracheal intubation, general anaesthesia …
An Hsi Report: Industrial Farm Animal Production And Livestock Associated Mrsa (Methicillin Resistant Staphylococcus Aureus),
2012
WellBeing International
An Hsi Report: Industrial Farm Animal Production And Livestock Associated Mrsa (Methicillin Resistant Staphylococcus Aureus), Humane Society International
HSI REPORTS
Staphylococcus aureus is a leading cause of bacterial infection and is increasingly found to be resistant to antibiotic therapy. A newly described type of Methicillin Resistant Staphylococcus aureus carried by farm animals, Livestock Associated MRSA (LA-MRSA), is now causing infections in humans with and without direct livestock contact. A reduction in the non-therapeutic use of antibiotics in feed would likely reduce the capacity of industrial animal agriculture to continue to create, disseminate, and perpetuate a large reservoir of LA-MRSA on a global scale, but more fundamental changes in the way animals are raised for food may be necessary forestall a …
Puppy Buyer Complaints: A Five-Year Summary, 2007‐2011,
2012
WellBeing International
Puppy Buyer Complaints: A Five-Year Summary, 2007‐2011, The Humane Society Of The United States
PUPPY MILL REPORTS
The Humane Society of the United States receives complaints about sick puppies on a daily basis. Over a five-year period (2007-2011), our puppy mills campaign received 2,479 puppy buyer complaints. The complaint came to The HSUS via our website complaint form (www.humanesociety.org/puppycomplaint), by email, and through our puppy mills tip line. Buyers complained about sick puppies sold by a variety of sources, including pet stores, breeders (both in person and online), or middleman dealers (sometimes known as brokers). Unsatisfied buyers contact The HSUS for many reasons; some are seeking information about how to pursue their consumer complaint, others …
Veterinary Problems In Puppy Mill Dogs,
2012
WellBeing International
Veterinary Problems In Puppy Mill Dogs, The Humane Society Of The United States
PUPPY MILL REPORTS
Dogs in puppy mills often suffer from an array of painful and potentially life-shortening veterinary problems due to overcrowded, unsanitary conditions and the lack of proper oversight or veterinary care. Conditions common to puppy mills, such as the use of stacked, wire cages to house more animals than a given space should reasonably hold, as well as constant exposure to the feces and urine of other dogs, make it difficult for dogs to avoid exposure to common parasites and infectious diseases. In addition, a lack of regular, preventive veterinary care, clean food and water, basic cleaning and grooming, and careful …
Clinical Anesthesia And Analgesia In Fish,
2012
University of Liverpool
Clinical Anesthesia And Analgesia In Fish, Lynne U. Sneddon
Veterinary Science and Medicine Collection
Fish have become a popular experimental model and companion animal, and are also farmed and caught for food. Thus, surgical and invasive procedures in this animal group are common, and this review will focus on the anesthesia and analgesia of fish. A variety of anesthetic agents are commonly applied to fish via immersion. Correct dosing can result in effective anesthesia for acute procedures as well as loss of consciousness for surgical interventions. Dose and anesthetic agent vary between species of fish and are further confounded by a variety of physiological parameters (e.g., body weight, physiological stress) as well as environmental …
Characterisation Of Chemosensory Trigeminal Receptors In The Rainbow Trout, Oncorhynchus Mykiss: Responses To Chemical Irritants And Carbon Dioxide,
2012
University of Liverpool
Characterisation Of Chemosensory Trigeminal Receptors In The Rainbow Trout, Oncorhynchus Mykiss: Responses To Chemical Irritants And Carbon Dioxide, Jessica J. Mettam, Catherine R. Mccrohan, Lynne U. Sneddon
Veterinary Science and Medicine Collection
Trigeminally innervated, mechanically sensitive chemoreceptors (M) were previously identified in rainbow trout, Oncorhynchus mykiss, but it is not known whether these receptors are responsive only to noxious, chemical irritants or have a general chemosensory function. This study aimed to characterise the stimulus–response properties of these receptors in comparison with polymodal nociceptors (P). Both P and M gave similar response profiles to acetic acid concentrations. The electrophysiological properties were similar between the two different afferent types. To determine whether the receptors have a nociceptive function, a range of chemical stimulants was applied to these receptors, including non-noxious stimuli such as ammonium …
Prevalence Of Antibodies To Canine Parvovirus And Distemper Virus In Wolves In The Canadian Rocky Mountains,
2012
University of Montana, Missoula
Prevalence Of Antibodies To Canine Parvovirus And Distemper Virus In Wolves In The Canadian Rocky Mountains, Brynn Nelson, Mark Hebblewhite, Vanessa Ezenwa, Todd Shury, Evelyn H. Merrill, Paul C. Paquet, Fiona Schmiegelow, Dale Selp, Geoff Skinner, Nathan Webb
Epidemiology Collection
Wild carnivores are often exposed to diseases via contact with peridomestic host species that travel through the wildland-urban interfaces. To determine the antibody prevalences and relationships to human activity for two common canid pathogens, we sampled 99 wolves (Canis lupus) from 2000 to 2008 for antibodies to canine parvovirus (CPV) and canine distemper virus (CDV) in Banff and Jasper National Parks and surrounding areas of the Canadian Rockies. This population was the source for wolves reintroduced into the Northern Rockies of the US. Of 99 wolves sampled, 94 had detectable antibody to CPV (95%), 24 were antibody-positive for CDV (24%), …
Mammary Cell Activity And Turnover In Dairy Cows Treated With The Prolactin-Release Inhibitor Quinagolide And Milked Once Daily, M. Boutinaud, V. Lollivier, L. Finot, R. M. Bruckmaier, P. Lacasse
Physiology Collection
To assess the regulation of mammary cell activity, survival, and proliferation by prolactin (PRL), 5 Holstein cows in early lactation received daily i.m. injections of 1 mg of quinagolide, a suppressor of PRL release, for 9 wk, whereas 4 control cows received the vehicle (water) only. During the last week of treatment, one udder half was milked once a day (1×) and the other twice a day (2×). Mammary biopsies were harvested 1 wk before and 4 and 8 wk after the start of quinagolide treatment. The quinagolide injections reduced milk yield and resulted in lower levels of κ-casein and …
Diagnosis Of Porcine Teschovirus
Encephalomyelitis In The Republic Of Haiti,
2012
U.S. Department of Agriculture
Diagnosis Of Porcine Teschovirus Encephalomyelitis In The Republic Of Haiti, Ming Y. Deng, Max Millien, Rodney Jacques-Simon, J. Keith Flanagan, Alexa J. Bracht, Consuelo Corrillo, Roger W. Barrette, Andrew Fabian, Fawzi Mohamed, Karen Moran, Jessica Rowland, Sabrina L. Swenson, Melinda Jenkins-Moore, Leo Koster, Bruce V. Thomsen, Gregory Mayr, Dave Pyburn, Paula Morales, John Shaw, Thomas Burrage, William White, Michael T. Mcintosh, Samia Metwally
Zoonotics and Wildlife Disease: Publications
In February and March 2009, approximately 1,500 backyard pigs of variable age became sick, and approximately 700 of them died or were euthanized in the Lower Artibonite Valley and the Lower Plateau of the Republic of Haiti. The main clinical sign was posterior ataxia followed by paresis and/or paralysis on the second or third day of illness. No gross lesions were observed at postmortem examinations. The morbidity and mortality were approximately 60% and 40%, respectively. Diagnostic samples (whole blood, brain, tonsil, lymph nodes, spleen, and lung) were negative for Classical swine fever virus and African swine fever virus. Porcine …
The Toxicity Of Acetaminophen, Caffeine And Carbendazim In Earthworms (Eisenia Fetida),
2012
Eastern Illinois University
The Toxicity Of Acetaminophen, Caffeine And Carbendazim In Earthworms (Eisenia Fetida), Matthew M. Bulman
Masters Theses
There has been a need for the assessment of ground water contamination risk on terrestrial organisms. One terrestrial organism, Eisenia fetida, was chosen as a test species and exposed to three common pharmaceutical and/or pesticide related contaminants: acetaminophen (CAS#103-90-2), caffeine (CAS#58-08-2) and carbendazim (CAS#10605-21-7), respectively. Levels of contamination varied from grams per liter to micrograms per liter (ppb) using distilled water as the solvent. Eisenia fetida was examined through a 28 day range/index toxicity test. A Benchmark Dose (BMD) dichotomous probit analysis, using a 95% confidence interval, was calculated for all three contaminants. All three contaminants were found to have …
Isospora Species (I. Canaria, Isospora Sp.) In Canaries (Serinus Canarius, Linnaeus),
2012
TÜBİTAK
Isospora Species (I. Canaria, Isospora Sp.) In Canaries (Serinus Canarius, Linnaeus), Cem Ecmel Şaki̇, Edi̇p Özer
Turkish Journal of Veterinary & Animal Sciences
Of 64 canaries with diarrhea in Elazığ Province between May 2002 and May 2006, 18 (28.1%) had Isospora oocysts in their feces. It was determined that the oocysts, examined morphologically before and after sporulating, were Isospora canaria and Isospora sp. Among the infected animals, 8 (44.4%) were concurrently infected with both species. Total numbers of I. canaria oocysts were greater than those of Isospora sp. A total of 2 canaries showing severe clinical signs were necropsied. Liver, spleen, and lung preparations and peripheral blood smears of the necropsied animals were examined for Atoxoplasma, and no development forms were seen. For …
Fractures In Young, Single-Humped Camels (Camelus Dromedarius),
2012
TÜBİTAK
Fractures In Young, Single-Humped Camels (Camelus Dromedarius), Ahmed F. Ahmed, Fahd Abdullah Al-Sobayil
Turkish Journal of Veterinary & Animal Sciences
The objectives were to study the frequencies and classification of fractures in young camels and to evaluate the clinical relevance of external fixation as a method of treatment. Cases of fractures (n = 75) in young camels (less than 2 years old) were studied. On admission, the cause, site, classification, and radiography of the fractures as well as the methods of treatment were investigated. Factors affecting fracture healing after treatment were investigated and analyzed. The frequencies of fracture were affected by breed (P = 0.001) and age (P = 0.01) but not sex. Trauma was the most common cause of …
Antioxidative And Antimicrobial Effects Of Garlic In Ground Camel Meat,
2012
TÜBİTAK
Antioxidative And Antimicrobial Effects Of Garlic In Ground Camel Meat, Hamid Reza Gheisari, Vahid Reza Ranjbar
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to investigate the antioxidative and antimicrobial effects of equivalent concentrations of garlic derivatives in ground camel meat during storage at 4 ± 1 °C. The addition of either garlic or butylated hydroxyanisole (BHA) (0.1 g/kg) significantly delayed lipid oxidation when compared with the control. The antioxidant activities of the various ingredients added followed the order of fresh garlic (FG), garlic powder (GP), BHA, and garlic oil (GO). After 14 days' storage, the aerobic plate count (APC) of both FG- and GP-formulated meat was significantly lower than that of either the control or BHA-formulated samples. …
Relations Between Electrical Conductivity, Somatic Cell Count, California Mastitis Test And Some Quality Parameters In The Diagnosis Of Subclinical Mastitis In Dairy Cows, Güven Kaşikçi, Ömer Çeti̇n, Enver Bariş Bi̇ngöl, Mehmet Can Gündüz
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to determine the effectiveness of the electrical conductivity (EC) method on the diagnosis of subclinical mastitis in dairy cows comparing with somatic cell count (SCC) and California mastitis test (CMT), and also to investigate the effect of these values on the amount of total viable bacteria, density, freezing point, and mineral substances. A total of 386 milk samples collected from quarters of 188 cows at 10 different farms were used as materials. Of the samples, 258 (66.85%) were CMT (+), 85 (22.02%) were CMT (++), and 43 (11.13%) were CMT (+++). The mean EC and SCC …
Effects Of Cold Stress During Transportation On Hatchability And Chick Quality Of Broiler Breeder Eggs,
2012
TÜBİTAK
Effects Of Cold Stress During Transportation On Hatchability And Chick Quality Of Broiler Breeder Eggs, Ahmad Salahi, Mozhdeh Moosanezhad Khabisi, Abbas Pakdel, Ali Baghbanzadeh
Turkish Journal of Veterinary & Animal Sciences
In this experiment 72,000 broiler breeder eggs (Ross 308 strain) collected from 36-week-old flocks were subjected to cold stress during transportation. Eggs were allocated to 4 temperature groups (treatments): 1.2 °C, 1-2 °C, 2.5-3.9 °C, 4-6 °C, and a control group, 21-22 °C. Each treatment had 14,400 eggs, and data were analyzed in a completely randomized design. The results of this study showed that cold stress had a significant effect on percentage of egg weight loss (P < 0.001), and minimal egg weight loss occurred in the control group. The percentage of exploders and early hatched chicks and chick weight were higher in the below zero temperature treatment than the other groups (P < 0.01). Cold stress had a significant effect on chick length, hatchability, and the hatching of fertile eggs (P < 0.001). The effects of cold stress on chick yield and body weight uniformity were significant (P < 0.01). The effect of cold stress on hatchery byproduct efficiency was significant (P < 0.001), but did not affect fertility. Cold stress also had significant effects on early (1-8 days), middle (9-17 days), and late mortality (20-21 days); total embryo mortality; and exposed brain. Ectopic viscera was significant (P < 0.001), and most mortality was observed in below 4 °C treatments. Total percentages of malpositions and deformity (P < 0.001) and egg contamination at 1-9 days (first stage) and 10-21 days (second phase) were affected by cold stress (P < 0.001). Cold stress also had a significant impact on the number of cull chicks; percent of string navel, button navel, total string, and button; omphalitis; full body cavity; red hocks; dehydration; dirty chickens; and stubby down. Cold stress affects performance during incubation and overall chick quality.
A Study On Paranal Sinus Micromorphometrical Parameters In Dogs Of Different Ages,
2012
TÜBİTAK
A Study On Paranal Sinus Micromorphometrical Parameters In Dogs Of Different Ages, Ivaylo Stefanov
Turkish Journal of Veterinary & Animal Sciences
This study determines the micromorphometric parameters of the main structural elements of the paranal sinus in male (n = 20) and female (n = 8) mongrel dogs of different ages. Pieces of about 1 cm3 were obtained from different parts of the organ and put in 10% neutral formalin. The tissue samples were further used for the preparation of paraffin sections that were stained with hematoxylin-eosin for light microscopy measurements. In the sinus wall, the thickness of the epithelium was measured, as was that of the subepithelial and subglandular connective tissue layers, the zona with the apocrine glands, and the …
