Immune Responses In Lactating Holstein Cows Supplemented With Cu, Mn, And Zn As Sulfates Or Methionine Hydroxy Analogue Chelates,
2012
University of Delaware
Immune Responses In Lactating Holstein Cows Supplemented With Cu, Mn, And Zn As Sulfates Or Methionine Hydroxy Analogue Chelates, L. M. Nemec, J. D. Richards, C. A. Atwell, D. E. Diaz, G. I. Zanton, T. F. Gressley
Physiology Collection
The aim of this study was to compare effects of inorganic sulfate versus chelated forms of supplemental Cu, Mn, and Zn on milk production, plasma and milk mineral concentrations, neutrophil activity, and antibody titer response to a model vaccination. Holstein cows (n = 25) were assigned in 2 cohorts based on calving date to a 12-wk randomized complete block design study. The first cohort consisted of 17 cows that had greater days in milk (DIM; mean of 77 DIM at the start of the trial) than the second cohort of 8 cows (32 DIM at the start of the trial). …
Expression And Distribution Of Thiol-Regulating Enzyme, Glutaredoxin 2 In Porcine Ocular Tissues,
2012
University of Nebraska-Lincoln
Expression And Distribution Of Thiol-Regulating Enzyme, Glutaredoxin 2 In Porcine Ocular Tissues, Bijaya Prasad Upadhyaya
School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research
Glutaredoxin 2 (Grx2), a thiol-regulating enzyme of oxidoreductase family and a mitochondrial isozyme of glutaredoxin 1, was discovered 11 years ago in our laboratory. Grx2 is present in the lens where it shows dethiolase, peroxidase, and ascorbate recycling activities. Recently, Grx2 has also been identified to protect the mitochondrial electron transport system with anti-apoptotic function. Since other eye tissues besides the lens are rich in mitochondria and are very sensitive to oxidative stress, we speculate the presence of Grx2 therein as an important redox regulator. This study is to investigate the expression and distribution of Grx2 in ocular tissues using …
Melanopsin Mediates Retrograde Visual Signaling In The
Retina,
2012
Oakland University
Melanopsin Mediates Retrograde Visual Signaling In The Retina, Dao-Qi Zhang, Michael A. Belenky, Patricia J. Sollars, Gary E. Pickard, Douglas G. Mcmahon
School of Veterinary and Biomedical Sciences: Faculty Publications
The canonical flow of visual signals proceeds from outer to inner retina (photoreceptors→bipolar cells→ganglion cells). However, melanopsin-expressing ganglion cells are photosensitive and functional sustained light signaling to retinal dopaminergic interneurons persists in the absence of rods and cones. Here we show that the sustained-type light response of retinal dopamine neurons requires melanopsin and that the response is mediated by AMPA-type glutamate receptors, defining a retrograde retinal visual signaling pathway that fully reverses the usual flow of light signals in retinal circuits.
Development Of One-Step Taqman® Real-Time Reverse Transcription-Pcr And Conventional Reverse Transcription-Pcr Assays For The Detection Of Equine Rhinitis A And B Viruses,
2012
University of Kentucky
Development Of One-Step Taqman® Real-Time Reverse Transcription-Pcr And Conventional Reverse Transcription-Pcr Assays For The Detection Of Equine Rhinitis A And B Viruses, Zhengchun Lu, Peter J. Timoney, Jena White, Udeni B R Balasuriya
Veterinary Science Faculty Publications
BACKGROUND: Equine rhinitis viruses A and B (ERAV and ERBV) are common equine respiratory viruses belonging to the family Picornaviridae. Sero-surveillance studies have shown that these two viral infections are prevalent in many countries. Currently, the diagnosis of ERAV and ERBV infections in horses is mainly based on virus isolation (VI). However, the sensitivity of VI testing varies between laboratories due to inefficient viral growth in cell culture and lack of cytopathic effect. Therefore, the objective of this study was to develop molecular diagnostic assays (real-time RT-PCR [rRT-PCR] and conventional RT-PCR [cRT-PCR] assays) to detect and distinguish ERAV from ERBV …
Animal Health Matters,
2012
South Dakota State University
Animal Health Matters, David H. Zeman, Russ Daly
Animal Health MATTERS Newsletter
[Page] 1- SDVMA and SDSU Initiate Stethoscope Ceremopny for Outgoing Pre-Veterinary Students [Page] 2- Director's Message: Planting Seeds for the Next Generation of Veterinarians in South Dakota [Page] 2- Dr. David Francis Honored at Retirement Ceremony [Page] 3- 2012-2013 SDSU Veterinary and Biomedical Sciences Department Scholarship Award Winners [Page] 4- Accounting and Human Resources Staff Keep the VBS Department Running [Page] 5- Pre-Veterinary Club Sparks SDSU One Health Coalition; Hosts One Health Commission CEO Pieces and Parts [Page] 5- Faculty Members Learn of Admission Changes at Iowa State University Regional Veterinary Education Conference [Page] 6- South Dakota Honors Veterinary and …
A Century And A Half Of Research On The Stable Fly, Stomoxys Calcitrans (L.) (Diptera: Muscidae), 1862-2011: An Annotated Bibliography,
2012
University of Nebraska-Lincoln
A Century And A Half Of Research On The Stable Fly, Stomoxys Calcitrans (L.) (Diptera: Muscidae), 1862-2011: An Annotated Bibliography, K. M. Kneeland, Steven R. Skoda, Jerome A. Hogsette, A. Y. Li, Jaime Molina-Ochoa, Kimberly Hutchinson Lohmeyer, John E. Foster
Department of Entomology: Faculty Publications
The stable fly, Stomoxys calcitrans, is a cosmopolitan pest of livestock, wild animals, pets, and humans. It is a primary pest of cattle in the United States, estimated to cause more than $1 billion in economic losses annually. It also causes dissension at the rural-urban interface and is a problem in recreation areas such as Florida beaches and the Great Lakes. Due to its pestiferous nature and painful bite, methods to control stable flies have been investigated for over a century. A large amount of research has been reported on stable fly biology, ecology, genetics, physiology, and vector competence. …
Growing South Dakota (Summer 2012),
2012
South Dakota State University
Growing South Dakota (Summer 2012), College Of Agriculture &. Biological Sciences
Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)
[Page] 2 150 Years and Counting: 2012 Marks a Significant Milestone for Land-Grant Universities [Page] 4 Summer College News
[Page] 6 Ready, Set, Grow: Gardening Programs Offer bevy of Benefits to Community
[Page] 8 SDSU Extension Staff Directory: Pull Out and Save for Future Reference
[Page] 13 Bugs Beware!: SDSU Assembles Talented Team of Entomologists
[Page] 14 High-Tech Teaching: Curriculums Now Integrate iPads, Social Media & Peer Mentoring
[Page] 16 A Lesson in Chinese: SDSU Contingent Experiences World's Largest Marketplace
[Page] 18 Here and There
[Page] 20 Adapting with Apps: Technological Advancements Offer Opportunities for Farming's Future
[Page] 21 A …
Role Of Matrix Metalloproteinases In Animals,
2012
Chulalongkorn University
Role Of Matrix Metalloproteinases In Animals, Sariya Asawakarn, Tanong Asawakarn
The Thai Journal of Veterinary Medicine
Matrix metalloproteinases are a family of calcium-dependent and zinc-dependent endopeptidases, which excrete from many cell types. These enzymes can hydrolyze extracellular matrix proteins. The most important inhibitor of MMPs is tissue inhibitors of metalloproteinases (TIMPs). The imbalance between MMPs and TIMPs causes pathological condition in human and animals. This review focuses on structural, role and several reports of MMPs in horse, dog, cat, fish and avian. Over production of MMPs is associated with tissue destruction and erosion of cartilage in equine osteoarthritis, especially MMP-2 and MMP-9. In canine and feline, many evidences reported about MMPs and cancer such as MMP-2 …
Risk Factors Of Subclinical Mastitis In Small Holder Dairy Cows In Khon Kaen Province,
2012
Chulalongkorn University
Risk Factors Of Subclinical Mastitis In Small Holder Dairy Cows In Khon Kaen Province, Chaiwat Jarassaeng, Suneerat Aiumlamai, Chalong Wachirapakorn, Mongkol Techakumphu, Jos P.T.M. Noordhuizen, Anton C. Beynen, Siriwat Suadsong
The Thai Journal of Veterinary Medicine
This cross-sectional study was introduced to determine the risk factors of subclinical mastitis in dairy farms. Sixteen small holder farms and 285 milking cows in Khon Kaen province were selected for the study. The mastitis investigation, feeding information and clinical inspection were applied in the farm visits. Milk samples from each quarter with a CMT score of 3 and bulk milk were collected for bacterial identification and somatic cell counts. The prevalence of subclinical mastitis and associated factors were analyzed. Results found that the prevalence of subclinical mastitis was 36.14%. Major microorganisms isolated from infected quarters were Streptococcus spp. and …
Duplex Pcr For Simultaneous And Unambiguous Detection Of Streptococcus Iniae And Streptococcus Agalactiae Associated With Streptococcosis Of Cultured Tilapia In Thailand,
2012
Chulalongkorn University
Duplex Pcr For Simultaneous And Unambiguous Detection Of Streptococcus Iniae And Streptococcus Agalactiae Associated With Streptococcosis Of Cultured Tilapia In Thailand, Channarong Rodkhum, Pattanapon Kayansamruaj, Nopadon Pirarat, Janenuj Wongtawatchai
The Thai Journal of Veterinary Medicine
Warm-water streptococcosis outbreaks in Thai cultured tilapia are caused by Streptococcus iniae and S.agalactiae. However, distinguishing between these two species of streptococcus are very difficult due to their similarmicrobiological appearance and clinical signs of infected fish. In this study, we proposed a new duplex-PCR basedmethod for simultaneous detection of these pathogens. The duplex-PCR amplified partial lctO and 16s rRNA gene ofS. iniae and S. agalactiae at 870 bp and 220 bp, respectively. This technique gave 100% specificity while sensitivity ofreaction was 100 fg of each bacterial genomic DNA. Detection limit of duplex-PCR applicable to clinical specimens isalso evaluated as 106 …
Early Selection According To Repeatability Of Body Measurements Of Turkish Arabian Foals,
2012
Chulalongkorn University
Early Selection According To Repeatability Of Body Measurements Of Turkish Arabian Foals, Süleyman Çilek
The Thai Journal of Veterinary Medicine
In this study, body measurements of Turkish Arabian foals at different ages was obtained from Anadolustate farm in Turkey which included body length, cannon bone circumference, heart girth and withers heightmeasurements. Effects of sex, birth month, birth year and age of foal at different growth periods on withers height,body length, heart girth and cannon bone circumference were significant (p<0.05). Effects of environmental factors onall body measurements were standardized additively. Using intra-class correlation method, repeatability was 0.55 forwither height, 0.47 for heart girth, 0.26 for cannon bone circumference for forelimb and 0.31 for body length.According to moderate level results of repeatability for especially heart girth and wither height measurements,selection of Turkish Arabian foals may be conducted at an early age according to body measurements at 6 months ofage.
Genetic Associations Between Stillbirth, Total Number Of Piglets Born And Gestation Length In A Commercial Pig Farm,
2012
Chulalongkorn University
Genetic Associations Between Stillbirth, Total Number Of Piglets Born And Gestation Length In A Commercial Pig Farm, Nalinee Imboonta, Prapatsara Kuhaaudomlarp
The Thai Journal of Veterinary Medicine
Data of purebred Landrace, Large White and their crossbreds taken from a commercial swine farm locatedin the north-eastern part of Thailand were used to evaluate the genetic associations of stillbirth with the total numberof piglets born and gestation length. The analyses were base on 28,275 litters born from 2002 to 2006. Analysis wascarried out with a multivariate animal model using average information REML procedures. Heritability estimates forstillbirth, the total number of piglets born and gestation length were 0.03±0.01, 0.03±0.01 and 0.16±0.01, respectively.The genetic correlation between the total number of piglets born and stillbirth was positive. The genetic correlationbetween the total …
Cloning And Prokaryotic Expression Of Cdnas From Hepatitis E Virus Structural Gene Of The Sw189 Strain,
2012
Chulalongkorn University
Cloning And Prokaryotic Expression Of Cdnas From Hepatitis E Virus Structural Gene Of The Sw189 Strain, Bao-Cheng Hao, Xi Lan, Xiao-Yong Xing, Hai-Tao Xiang, Feng-Qin Wen, Yu-Yao Hu, Yong-Hao Hu, Jian-Ping Liang, Ji-Xing Liu
The Thai Journal of Veterinary Medicine
It is necessary to study the second open reading frame (open reading frame 2, ORF2) and antigenic peptidescharacteristic of genotype 4 of HEV, and create one kind of high sensitivity and specificity of anti-HEV IgM detectionkit. The objective of this study was to obtain recombinant antigen for development of anti-HEV ELISA method andvaccine against hepatitis E virus infection. A 728 base cDNA was collected from 5'-terminus of open reading frame 2(ORF2) among epidemic hepatitis E virus (HEV) isolated from Gansu, Western China. The fragment was digestedwith Not I and Nco I, and inserted into vector pET32a(+). The recombinant plasmid was …
Seasonal Monitoring Of Dengue Infection In Aedes Aegypti And Serological Feature Of Patients With Suspected Dengue In 4 Central Provinces Of Thailand,
2012
Chulalongkorn University
Seasonal Monitoring Of Dengue Infection In Aedes Aegypti And Serological Feature Of Patients With Suspected Dengue In 4 Central Provinces Of Thailand, Jakkrawarn Chompoosri, Usavadee Thavara, Apiwat Tawatsin, Surapee Anantapreecha, Padet Siriyasatien
The Thai Journal of Veterinary Medicine
This study aimed to determine seasonal dengue infection rates in Ae. aegypti mosquitoes and dengueinfection in suspected patients in 4 central provinces of Thailand. Ae. aegypti mosquitoes collected during threeseasons and blood specimens taken from patients with suspected dengue were detected and serotyped for dengueviruses using RT-PCR. The biting behavior of Ae. aegypti females was studied by 24-hour mosquitoes collection once amonth using human bait for determination of seasonal biting rate. Dengue infection rates in Ae. aegypti femalesobtained from all the 4 provinces were highest in hot season and varied from place to place ranging from 64.4% to77.5%, whereas morbidity …
A Comparative Study On The Efficacy Of Four Semen Extenders And Thawing By Seminal Plasma On The Quality Of Frozenthawed Boar Semen,
2012
Chulalongkorn University
A Comparative Study On The Efficacy Of Four Semen Extenders And Thawing By Seminal Plasma On The Quality Of Frozenthawed Boar Semen, Chonthida Rienprayoon, Chonlapat Klangnak, Suttiporn Onton, Chanyuth Tretipskul, Padet Tummaruk
The Thai Journal of Veterinary Medicine
The present study investigated the influence of four different commercial semen extenders used during theholding time and the use of seminal plasma as a thawing medium on the frozen-thawed (FT) boar semen quality.Sperm-rich fractions were collected from 11 boars and were divided into four groups according to the type of semenextender used during the holding time (A, B, C and D). The extended semen was cooled down and centrifuged. Thesperm pellets were diluted in lactose-egg yolk extender plus glycerol and Equex STM Paste. The semen were loadedinto 0.5 ml straws and frozen. Thawing was achieved by immersing the straws in …
Acute And Subchronic Toxicity Study Of Ardisia Ellipica Thunb. Fruit Extract,
2012
Chulalongkorn University
Acute And Subchronic Toxicity Study Of Ardisia Ellipica Thunb. Fruit Extract, Nalinphat Saktiyasunthorn, Songpol Chivapat, Pornchai Sincharoenpokai, Anudep Rungsipipat, Nongnuth Maneechai, Boonyanee Suphaphon, Aussavashai Shuayprom
The Thai Journal of Veterinary Medicine
Several plants species in the genus Ardisia contained various biologically active compounds. Ram Yai orPilangkasa (Ardisia elliptica Thunb.) has been used in traditional medicines. The objective of this study was to evaluatethe safety of ethanolic extract of A. elliptica fruit in animal models by oral administration. Acute toxicity test in miceby gavage the extract at each dose of 2.5 g/kg twice revealed no abnormal signs, mortality and gross lesion of vitalorgans. Subchronic toxicity study was investigated in one hundred Wistar rats separated into five groups, each oftwenty (ten males and ten females). Two control groups received distilled water and 0.5% …
The Depletion Of Intracellular Glutamine By Methionine Sulfoximine On Amino Acid Uptake In Placental Cells (Bewo),
2012
Chulalongkorn University
The Depletion Of Intracellular Glutamine By Methionine Sulfoximine On Amino Acid Uptake In Placental Cells (Bewo), Boonrit Thongsong
The Thai Journal of Veterinary Medicine
The intracellular concentration of glutamine is very high where it plays a central role as a ready source ofnitrogen, carbon and energy in various metabolic processes. The purpose of the present study was to investigate theinfluence of intracellular glutamine status on amino acid uptake activity in placental choriocarcinoma (BeWo) cells,analogous to normal placental trophoblasts. Intracellular glutamine was depleted by culturing the cells in regularmedium without glutamine and by treating with 2 mM methionine sulfoximine (MSX), an inhibitor of glutaminesynthetase, for 16 hours. The uptake of various amino acids was measured by the use of appropriate substrates andionic conditions. When cultured …
Seroprevalence Of Neospora Caninum In Swamp Buffaloes And Beef Cattle In The North-East Of Thailand,
2012
Chulalongkorn University
Seroprevalence Of Neospora Caninum In Swamp Buffaloes And Beef Cattle In The North-East Of Thailand, Hoai Nam Nguyen, Aran Chanlun, Kwankate Kanistanon, Suneerat Aiumlamai
The Thai Journal of Veterinary Medicine
A seroepidemiological survey was carried out to investigate seroprevalence of Neospora caninum in swamp buffaloes from five provinces in north-eastern Thailand, and to compare infection status in swamp buffaloes and beef cattle at one area of Thailand. A single serum sample was collected from each of 532 swamp buffaloes and 78 beef cattle during January and July 2009. Antibodies against N. caninum were analyzed by a commercial ELISA test. Chisquare tests were used to compare the seroprevalence between species, gender and age groups in Khon Kaen. Result showed that infection prevalence in swamp buffaloes in northeastern Thailand was 4.5%. At …
Prevalence Of Gastrointestinal Parasites In Yellow Cattle Between Taiwan And Its Offshore Islands,
2012
Chulalongkorn University
Prevalence Of Gastrointestinal Parasites In Yellow Cattle Between Taiwan And Its Offshore Islands, Kwong-Chung Tung, Chiu-Chen Huang, Chien-Hung Pan, Cheng-Hsiung Yang, Cheng-Hung Lai
The Thai Journal of Veterinary Medicine
A total of 310 fecal samples of yellow cattle were collected in Taiwan and its offshore islands Penghu andKinmen for gastrointestinal parasite examination using coprological techniques. The overall prevalence was 73.2%.The infection rates of protozoa, nematodes, trematodes, and cestodes were 57.7%, 37.7%, 17.1%, and 0.6%,respectively. Among all parasites, Cryptosporidium spp. (41.6%) were the most predominant, followed by strongyles(36.1%) and Eimeria spp. (11.9%). There were significant differences in the prevalence of protozoa and nematodesbetween different distributional area groups. The present study demonstrates that gastrointestinal parasiticinfections, particularly protozoan infections, occur frequently in yellow cattle in Taiwan and its offshore islands. Theresults indicate …
The Therapeutic Effects Of Prednisolone In Cats With Immune-Mediated Hemolytic Anemia,
2012
Chulalongkorn University
The Therapeutic Effects Of Prednisolone In Cats With Immune-Mediated Hemolytic Anemia, Rosama Pusoonthornthum, Parkorn Limlenglert, Pitcha Pornmingmas, Anudep Rungsipipat, Pinit Pusoonthornthum
The Thai Journal of Veterinary Medicine
The present study was performed to find the therapeutic effects of 2 mg/kg prednisolone orally fed twice a day in cats with immune-mediated hemolytic anemia (IMHA). Six IMHA positive cats were diagnosed with Coomb’s test. Mean age of IMHA cats was 4.4 years old. They were considered as having secondary IMHA. The mortality rate of IMHA cats received prednisolone treatment was 33.3%. Complete blood count and blood chemistry values were all within the normal limit.
