Immunization Of West Nile Recombinant Envelope Domain Iii With Equine Cd40 Ligand Protein Vaccine Induced Specific Immune Response In Rabbits And Horses,
2012
Louisiana State University and Agricultural and Mechanical College
Immunization Of West Nile Recombinant Envelope Domain Iii With Equine Cd40 Ligand Protein Vaccine Induced Specific Immune Response In Rabbits And Horses, Shiliang Anthony Liu
LSU Master's Theses
West Nile virus (WNV) is one of several flaviruses known to infect mammalian species, including humans. There were 15,257 horse cases reported in 2002 and 1,086 in 2006 in United States. Recently, significant increases in equine and human cases have been reported in United States. Domain III of the WNV envelope protein binds to cellular receptors, and induces a significant portion of the neutralizing antibody response against the virus. CD40 Ligand (CD40L, CD154) enhances productive interactions between T cells and APC and has been shown to function as a potential adjuvant. In this study, we constructed and expressed a fusion …
The Effects Of Iodixanol Present During Equine Semen Cryopreservation,
2012
Louisiana State University and Agricultural and Mechanical College
The Effects Of Iodixanol Present During Equine Semen Cryopreservation, David Paul Beehan
LSU Master's Theses
The objectives of this study were to determine what effects iodixanol would have on total and progressive motility, plasma membrane integrity (viability), acrosome integrity and DNA structure when present during cryopreservation of equine spermatozoa,. We hypothesized that the addition of iodixanol would improve post-thaw values for measured parameters. Ejaculates from six stallions were collected, centrifuged at 900 x g for ten minutes to remove supernatant, and suspended to 200 x 106 cells/ml with 0%, 2.5% and 5% iodixanol in an egg-yolk based extender and cryopreserved. Before and after cryopreservation sperm motility was assessed by computer assisted semen analysis, and samples …
Evaluation Of Plasma Sprayed Hydroxyapatite Coated Ao Cortical Screws In Equine Third Metacarpal Bone,
2012
Louisiana State University and Agricultural and Mechanical College
Evaluation Of Plasma Sprayed Hydroxyapatite Coated Ao Cortical Screws In Equine Third Metacarpal Bone, Myra Elizabeth Durham
LSU Master's Theses
ABSTRACT Objectives - To compare the osteointegration of plasma sprayed hydroxyapatite (HA) coated and uncoated 5.5 mm cortical screws in equine third metacarpal (MC3) bones in combination with a 4.5mm broad dynamic compression plate (DCP). Study design - In vivo study. Animals – 6 Adult Thoroughbred horses. Methods – For each horse, four HA coated screws were placed unicortically through a 4.5mm broad DCP in the dorsal cortex of a randomly chosen MC3 bone, with 4 uncoated screws placed in an identical manner in the contralateral MC3 bone. All screws were tightened to a torque of 5.4 N-m. Extraction torques …
Structural And Molecular Pathology Of The Atrium In Boxer Arrhythmogenic Cardiomyopathy,
2012
Louisiana State University and Agricultural and Mechanical College
Structural And Molecular Pathology Of The Atrium In Boxer Arrhythmogenic Cardiomyopathy, Jorge Luis Vila
LSU Master's Theses
Arrhythmogenic cardiomyopathy (AC), also known as arrhythmogenic right ventricular cardiomyopathy (ARVC), is a disease characterized by fatty or fibro-fatty myocardial replacement, predominantly in the right ventricle and to a lower extent the left ventricle. It is recognized as a disease affecting the cardiac intercalated disc. Clinically, it is associated with ventricular arrhythmias, although atrial arrhythmias and atrial histopathological changes characteristic of AC have occasionally been reported. The full extent of atrial involvement in AC has not been investigated. Therefore, we aimed to apply histopathology, immunochemical detection, immunolocalization and transmission electron microscopy (TEM) techniques to characterize the distribution of desmosomal and …
Estimation Of Variance Components And Genetic Parameters For Type Traits And Milk Yield In Holstein Cattle,
2012
TÜBİTAK
Estimation Of Variance Components And Genetic Parameters For Type Traits And Milk Yield In Holstein Cattle, Serdar Duru, Salahatti̇n Kumlu, Erdoğan Tuncel
Turkish Journal of Veterinary & Animal Sciences
This research was conducted to estimate variance components and genetic parameters for type traits and milk yield in Holstein-Friesian cattle. In this study, 597 daughters of 158 sires in 128 herds were classified. In the data analysis, type scores for 354 daughters bred within 70 herds sired by 46 sires that had at least 3 daughters, and 304 lactation records for 206 daughters within 56 herds sired by 37 sires, were considered. For estimation of variance components and correlations among the traits, the MTDFREML package program was used. The mean stature was 145.56 cm. Means for linear traits varied from …
Rhodotorula Minuta Fungemia In A Ewe Lamb,
2012
USDA-ARS
Rhodotorula Minuta Fungemia In A Ewe Lamb, C G. Chitko-Mckown, K A. Leymaster, M. P. Heaton, D D. Griffin, J K. Veatch, S A. Jones, M. L. Clawson
School of Veterinary and Biomedical Sciences: Faculty Publications
An 8-month-old crossbred ewe, normal upon physical examination, was humanely euthanized for tissue collection. After approximately 3 weeks in tissue culture, fungi began budding out of cells obtained from the choroid plexus. After an additional 3 weeks, budding was observed in kidney cell cultures and eventually in monocyte cultures as well. Serum from the lamb was submitted to the Veterinary Diagnostic Laboratory at Colorado State University for fungal diagnosis and was found negative for Aspergillus, Blastomyces, Coccidioidomycosis and Histoplasmosis. DNA was isolated from fungi collected from tissue culture supernatants and used in a set of pan-fungal PCR assays with DNA …
Posttranslational Modification Of Vesicular Stomatitis Virus
Glycoprotein, But Not Jnk Inhibition, Is The Antiviral Mechanism
Of Sp600125,
2012
Technical University of Munich
Posttranslational Modification Of Vesicular Stomatitis Virus Glycoprotein, But Not Jnk Inhibition, Is The Antiviral Mechanism Of Sp600125, Sabrina Marozin, Jennifer Altomonte, Sibylle Apfel, Phat X. Dinh, Enrico De Toni, Antonia Rizzani, Andreas Nüssler, Nobuyuki Kato, Roland M. Schmid, Asit K. Pattnaik, Oliver Eberta
School of Veterinary and Biomedical Sciences: Faculty Publications
Vesicular stomatitis virus (VSV), a negative-sense single-stranded-RNA rhabdovirus, is an extremely promising oncolytic agent for cancer treatment. Since oncolytic virotherapy is moving closer to clinical application, potentially synergistic combinations of oncolytic viruses and molecularly targeted antitumor agents are becoming a meaningful strategy for cancer treatment. Mitogenactivated protein kinase (MAPK) inhibitors have been shown to impair liver cell proliferation and tumor development, suggesting their potential use as therapeutic agents for hepatocellular carcinoma (HCC). In this work, we show that the impairment of MAPK in vitro did not interfere with the oncolytic properties of VSV in HCC cell lines. Moreover, the administration …
Identification Of Amino Acid Residues Important For Anti-Ifn Activity
Of Porcine Reproductive And Respiratory Syndrome Virus
Non-Structural Protein 1,
2012
University of Nebraska-Lincoln
Identification Of Amino Acid Residues Important For Anti-Ifn Activity Of Porcine Reproductive And Respiratory Syndrome Virus Non-Structural Protein 1, Lalit Beura, Sakthivel Subramaniam, Hiep Vu, Byungjoon Kwon, Asit K. Pattnaik, Fernando A. Osorio
School of Veterinary and Biomedical Sciences: Faculty Publications
The non-structural protein 1 (nsp1) of porcine reproductive and respiratory syndrome virus is partly responsible for inhibition of type I interferon (IFN) response by the infected host. By performing alanine-scanning mutagenesis, we have identified amino acid residues in nsp1α and nsp1β~ (the proteolytic products of nsp1) that when substituted with alanine(s) exhibited significant relief of IFNsuppression. A mutant virus (16-SA, in which residues 16-20 of nsp1β were substituted with alanines) encoding mutant nsp1β recovered from infectious cDNA clone was shown to be attenuated for growth in vitro and induced significantly higher amount of type I IFN transcripts in infected macrophages. …
A Single Amino Acid Change Resulting In Loss Of Fluorescence Of Egfp In A Viral
Fusion Protein Confers Fitness And Growth Advantage To The Recombinant
Vesicular Stomatitis Virus,
2012
University of Nebraska-Lincoln
A Single Amino Acid Change Resulting In Loss Of Fluorescence Of Egfp In A Viral Fusion Protein Confers Fitness And Growth Advantage To The Recombinant Vesicular Stomatitis Virus, Phat X. Dinh, Debasis Panda, Phani B. Das, Subash C. Das, Anshuman Das, Asit K. Pattnaik
School of Veterinary and Biomedical Sciences: Faculty Publications
Using a recombinant vesicular stomatitis virus encoding eGFP fused in-frame with an essential viral replication protein, the phosphoprotein P, we show that during passage in culture, the virus mutates the nucleotide C289 within eGFP of the fusion protein PeGFP to A or T, resulting in R97S/C amino acid substitution and loss of fluorescence. The resultant non-fluorescent virus exhibits increased fitness and growth advantage over its fluorescent counterpart. The growth advantage of the non-fluorescent virus appears to be due to increased transcription and replication activities of the PeGFP protein carrying the R97S/C substitution. Further, our results show that the R97S/C mutation …
Amino Acid Residues In The Non-Structural Protein 1 Of Porcine Reproductive
And Respiratory Syndrome Virus Involved In Down-Regulation Of Tnf-Cx
Expression In Vitro And Attenuation In Vivo,
2012
University of Nebraska-Lincoln
Amino Acid Residues In The Non-Structural Protein 1 Of Porcine Reproductive And Respiratory Syndrome Virus Involved In Down-Regulation Of Tnf-Cx Expression In Vitro And Attenuation In Vivo, Sakthivel Subramaniam, Lalit Beura, Byungjoon Kwon, Asit K. Pattnaik, Fernando A. Osorio
School of Veterinary and Biomedical Sciences: Faculty Publications
Porcine reproductive and respiratory syndrome virus (PRRSV) suppresses tumor necrosis factor-alpha (TNF-α) production at both transcriptional and post-transcriptional levels by its non-structural proteins 1α and 1β (Nsp1α and Nsp1β). To identifY the amino acid residues responsible for this activity, we generated several alanine substitution mutants of Nsp1α and Nsp1β. Examination of the mutant proteins revealed that Nsp1α residues Gly90, Asn91 , Arg97, Argl 00 and Arg124 were necessary for TNF-α promoter suppression, whereas several amino acids spanning the entire Nsp1β ~ were found to be required for this activity. Two mutant viruses, with mutations at Nsp1α Gly90 or Nsp1β residues …
Staphylococcal Response To Oxidative Stress,
2012
University of Nebraska - Lincoln
Staphylococcal Response To Oxidative Stress, Rosmarie Gaupp, Nagender Ledala, Greg A. Somerville
School of Veterinary and Biomedical Sciences: Faculty Publications
Staphylococci are a versatile genus of bacteria that are capable of causing acute and chronic infections in diverse host species. The success of staphylococci as pathogens is due in part to their ability to mitigate endogenous and exogenous oxidative and nitrosative stress. Endogenous oxidative stress is a consequence of life in an aerobic environment; whereas, exogenous osidative and nitrosative stress are often due to the bacteria's interaction with host immune systems. To overcome the deleterious effects of oxidative and nitrosative stress, staphylococci have evolved protection, detoxification, and repair mechanisms that are controlled by a network of regulators. In this review, …
Serum Protein Fractions In Brazilian-Breed Donkeys Using Agarose Gel Electrophoresis,
2012
TÜBİTAK
Serum Protein Fractions In Brazilian-Breed Donkeys Using Agarose Gel Electrophoresis, Paulo Henrique Cavalcante, Andrea Cristina Silva, Sidnei Sakamoto, Benito Soto-Blanco
Turkish Journal of Veterinary & Animal Sciences
The values for the serum protein profiles of horses are available in many studies. However, only one study has characterized the electrophoretic migration profile of plasma protein in donkeys. The present study aimed to determine by agarose gel electrophoresis the normal values for serum protein fractions in erratic Brazilian-breed donkeys and to compare these values with those of the serum proteins of horses. Serum samples were collected from 26 donkeys of the Brazilian-breed and from 10 healthy horses. The serum levels of the total proteins were determined by the biuret method. The separation and determination of the values of serum …
Caesarean Operation In Two Farmed Red Deer (Cervus Elaphus),
2012
TÜBİTAK
Caesarean Operation In Two Farmed Red Deer (Cervus Elaphus), Si̇nem Özlem Engi̇nler, Mehmet Can Gündüz, Tuğba Seval Fatma Toydemi̇r, Meli̇h Uçmak, Güven Kaşikçi
Turkish Journal of Veterinary & Animal Sciences
In this case, a caesarean section was performed on 2 red deer aged 4 and 7 who underwent dystocia. When the deer were brought to our clinic, they had been in labour for 6 h and 1 day, respectively, and the calves were dead. The foetal forefeet were found hanging from the vulva at the general examination. Deer 1's parturition was human-assisted but the outcome was not good and it was decided to operate on the animal. In deer 2, parturition was taking too long and so it was decided to operate immediately. The deer were sedated and anaesthetised by …
Survival And Growth Of Common Carp (Cyprinus Carpio L.) Exposed To Different Water Ph Levels,
2012
TÜBİTAK
Survival And Growth Of Common Carp (Cyprinus Carpio L.) Exposed To Different Water Ph Levels, Mohammad Saeed Heydarnejad
Turkish Journal of Veterinary & Animal Sciences
The aim of the present study was to examine the survival and growth performance of common carp (Cyprinus carpio L.) in water with different pH values. Carp (17.8 ± 1.21 cm; 52.14 ± 7.13 g) were transferred to 21 tanks previously adjusted with 7 different pH values: 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, and 9.0. Each value had 3 replicates, and all experiments lasted for 21 days. The survival and growth of carp were assessed at days 7, 14, and 21. At the end of the 21 days, greater weight, length, survival, and biomass were found for pH values of …
Prevalence Of Escherichia Coli O157 In Red Meat And Meat Products Determined By Vidas Ecpt And Lightcycler Pcr,
2012
TÜBİTAK
Prevalence Of Escherichia Coli O157 In Red Meat And Meat Products Determined By Vidas Ecpt And Lightcycler Pcr, Seran Temelli̇, Ayşe Gül Eyi̇gör, Şahsene Anar
Turkish Journal of Veterinary & Animal Sciences
This study aimed to determine the prevalence of Escherichia coli O157 in retail red meat and meat products with the Vitek Immunodiagnostic Assay System, including H7 Escherichia coli phage technology (VIDAS ECPT), and a real-time polymerase chain reaction system (LightCycler PCR; LCPCR). A total of 106 red meat and meat product samples were analyzed with VIDAS ECPT and LCPCR. VIDAS ECPT presumptive positive samples were subjected to VIDAS Immuno Concentration E. coli O157, followed by culture and serology. Among the 27 out of 72 (37.50%) red meat samples and 3 out of 34 (8.82%) red meat products that tested positive …
Evaluation Of The Possibility Of Using Oligosaccharide-Free Pea In Japanese Quail Nutrition,
2012
TÜBİTAK
Evaluation Of The Possibility Of Using Oligosaccharide-Free Pea In Japanese Quail Nutrition, Danuta Szczerbinska, Zofia Tarasewicz, Piotr Gulewicz, Danuta Majewska, Marek Ligocki
Turkish Journal of Veterinary & Animal Sciences
In this paper, evaluation of the effect of processed pea seeds (free of raffinose family oligosaccharides) on the performance traits and the results of reproduction in Japanese quails is presented. A flock of 72 quails was divided into 3 groups consisting of equal numbers of birds (18 females and 6 males). Birds were kept in cages in an environmentally controlled room with a lighting cycle of 17 h of light and 7 h of dark, and they were fed 1 of 3 different diets. Whereas diet 1 served as the control, diet 2 was supplemented with 5% and diet 3 …
Molecular And Kinetic Characterization Of Lactate Dehydrogenase Enzyme In The Heart And Breast Muscle Of Japanese Quail (Coturnix Japonica), Ram Pratap Singh, Kochiganti Venkata Hanumat Sastry, Radha Agarwal, Nitin Kumar Pandey, Vishesh Kumar Saxena, Jag Mohan
Turkish Journal of Veterinary & Animal Sciences
In the present investigation, attempts were made to characterize lactate dehydrogenase (LDH) isozyme in quail heart and breast muscle by using electrophoresis, kinetic, and molecular techniques. Polyacrylamide gel electrophoresis of the heart and breast muscle revealed a single isozyme. Kinetic studies were carried out for further functional characterization of LDH isozyme in the heart and breast muscle. Substrate inhibition, enzyme kinetics, and inhibitor (urea) studies indicated that the isozymes present in these tissues differed in their functional properties. LDH isozyme present in the heart and breast muscle exhibited properties of LDH-A and LDH-B isozyme, respectively. Furthermore, RT-PCR amplification of Ldh-a …
Urinary Diversion Via Preputial Urethrostomy With Bilateral Pubic-Ischial Osteotomy In A Dog,
2012
TÜBİTAK
Urinary Diversion Via Preputial Urethrostomy With Bilateral Pubic-Ischial Osteotomy In A Dog, Masaaki Katayama, Yasuhiko Okamura, Hiroaki Kamishina, Yuji Uzuka
Turkish Journal of Veterinary & Animal Sciences
A 4-year-old, 8.7-kg intact male Pekingese dog was presented with stricture of the urethral opening after a perineal urethrostomy revision surgery. Total penile amputation and preputial urethrostomy with bilateral pubic-ischial osteotomy were performed. After surgery, the dog was able to urinate through the natural preputial orifice, avoiding urine scalding associated with urethral anastomosis to the adjacent skin. Nine months after surgery, no major complications were noted with the exception of a small amount of urine dribbling after urination.
Effects Of Dietary Protein Supplementation On The Performance Of West African Dwarf (Wad) Goats Infected With Haemonchus Contortus And Trichostrongylus Colubriformis, Linus Iorundu Mhomga, Pius Ajanwachukwu Nnadi, Samuel Nnegbo Chiejina, Idika Kalu Idika, Lucas Atehmengo Ngongeh
Turkish Journal of Veterinary & Animal Sciences
Twenty worm-free male West African dwarf (WAD) goats, 7-8 months of age, were used to study the effect of dietary protein on their response to mixed infections of trychostrongyles. The goats were divided into 4 groups (A-D), each containing 5 animals. Groups A and B were fed forages with concentrate feed containing 17.06% crude protein, whereas Groups C and D were fed forages alone. An escalating infection of 500 infective larvae (L3), 1000 L3, 2000 L3, and 4000 L3 was given consecutively at weeks 0, 1, 2, and 3, respectively, for 4 weeks to groups A and D. Patency and …
Oncogenic Viruses And Mechanisms Of Oncogenesis,
2012
TÜBİTAK
Oncogenic Viruses And Mechanisms Of Oncogenesis, Murat Şevi̇k
Turkish Journal of Veterinary & Animal Sciences
In this article, current information about oncogenic DNA and RNA viruses and their oncogenesis mechanisms that cause tumors and cancers in animals and humans is presented.
