Reproductive Performance Of Primiparous And Multiparous Saanen Goats After Laparoscopic Intrauterine Insemination: A Field Study, Recai̇ Kulaksiz, Ali̇ Daşkin
Turkish Journal of Veterinary & Animal Sciences
The objective of this study was to evaluate the reproductive performance of primiparous and multiparous Saanen goats after intrauterine laparoscopic artificial insemination with frozen semen. Twenty-four Saanen goats, divided in 2 groups: group 1 consisted of 11 primiparous goats and group 2 consisted of 13 multiparous goats. Estrus was synchronized by 20 mg fluorogestone acetate (FGA)-impregnated intravaginal sponges and the IM administration of 125 mg of cloprostenol (PGF2\alpha) and eCG (400 IU), 48 h before sponge removal. Intrauterine fixed time artificial insemination (AI) was carried out 55 h after removal of the FGA sponges. Results of the present study indicate …
A Loop-Mediated Isothermal Amplification (Lamp) Assay For Rapid Detection Of Anaplasma Phagocytophilum Infection In Dogs,
2012
TÜBİTAK
A Loop-Mediated Isothermal Amplification (Lamp) Assay For Rapid Detection Of Anaplasma Phagocytophilum Infection In Dogs, Chungchen Lee, Yungchang Lin, Chauloong Tsang, Yangtsung Chung
Turkish Journal of Veterinary & Animal Sciences
A simple and rapid diagnostic method of Anaplasma phagocytophilum infection was established, employing the loop-mediated isothermal amplification (LAMP) reaction. With 1 set of 4 specific primers targeting the citrate synthase (gltA) gene of A. phagocytophilum, the LAMP assay rapidly amplified the target DNA sequence in a single tube in 1 h, requiring only a water bath for reaction. When visualized by gel electrophoresis, the LAMP products appeared as a ladder-like pattern, with many bands of different sizes from 180 base pairs up to the loading well. The results obtained from testing 32 clinical blood samples of dogs demonstrated that the …
Evaluation Of Elevated Bait Trays For Attracting Blackbirds (Icteridae) In Central
North Dakota,
2012
USDA/APHIS/WS National Wildlife Research Center
Evaluation Of Elevated Bait Trays For Attracting Blackbirds (Icteridae) In Central North Dakota, George M. Linz, Jamison B. Winter, William J. Bleier
United States Department of Agriculture Wildlife Services: Staff Publications
Sunflower (Helianthus annuus L.) became an economically important crop in North Dakota in the 1970s, providing a major source of food for post-breeding blackbirds (Icteridae). Reducing local blackbird populations with rice grains treated with an avicide is one proposed alternative for reducing sunflower damage. In fall 2007 and 2008, we evaluated the idea of attracting blackbirds to rice-baited trays attached to wire cages supplied with live blackbirds. During our observations (1011 h), we saw 3888 birds, consisting of 25 species and 12 families, on the bait trays. Blackbirds made up 90.4% of the bird observations, whereas sparrows (Emberizidae) made …
Copper–Zinc Superoxide Dismutase-Deficient Mice Show
Increased Susceptibility To Experimental Autoimmune
Encephalomyelitis Induced With Myelin Oligodendrocyte
Glycoprotein 35–55,
2012
University of Nebraska-Lincoln
Copper–Zinc Superoxide Dismutase-Deficient Mice Show Increased Susceptibility To Experimental Autoimmune Encephalomyelitis Induced With Myelin Oligodendrocyte Glycoprotein 35–55, Chandirasegara Massilamany, Arunakumar Gangaplara, Heejeong Kim, Charlotte Standord, Govardhan Rathnaiah, David Steffen, Jaekwon Lee, Jay Reddy
School of Veterinary and Biomedical Sciences: Faculty Publications
In this report, we have addressed the role of copper–zinc superoxide dismutase (SOD1) deficiency in the mediation of central nervous system autoimmunity. We demonstrate that SOD1-deficient C57Bl/6 mice develop more severe autoimmune encephalomyelitis induced with myelin oligodendrocyte glycoprotein (MOG) 35–55, compared with wild type mice. This alteration in the disease phenotype was not due to aberrant expansion of MOG-specific T cells nor their ability to produce inflammatory cytokines; rather lymphocytes generated in SOD1-deficient mice were more prone to spontaneous cell death when compared with their wild type littermate controls. The data point to a role for SOD1 in the maintenance …
Rhodotorula Minuta Fungemia In A Ewe Lamb,
2012
USDA-ARS
Rhodotorula Minuta Fungemia In A Ewe Lamb, C G. Chitko-Mckown, K A. Leymaster, M. P. Heaton, D D. Griffin, J K. Veatch, S A. Jones, M. L. Clawson
School of Veterinary and Biomedical Sciences: Faculty Publications
An 8-month-old crossbred ewe, normal upon physical examination, was humanely euthanized for tissue collection. After approximately 3 weeks in tissue culture, fungi began budding out of cells obtained from the choroid plexus. After an additional 3 weeks, budding was observed in kidney cell cultures and eventually in monocyte cultures as well. Serum from the lamb was submitted to the Veterinary Diagnostic Laboratory at Colorado State University for fungal diagnosis and was found negative for Aspergillus, Blastomyces, Coccidioidomycosis and Histoplasmosis. DNA was isolated from fungi collected from tissue culture supernatants and used in a set of pan-fungal PCR assays with DNA …
Posttranslational Modification Of Vesicular Stomatitis Virus
Glycoprotein, But Not Jnk Inhibition, Is The Antiviral Mechanism
Of Sp600125,
2012
Technical University of Munich
Posttranslational Modification Of Vesicular Stomatitis Virus Glycoprotein, But Not Jnk Inhibition, Is The Antiviral Mechanism Of Sp600125, Sabrina Marozin, Jennifer Altomonte, Sibylle Apfel, Phat X. Dinh, Enrico De Toni, Antonia Rizzani, Andreas Nüssler, Nobuyuki Kato, Roland M. Schmid, Asit K. Pattnaik, Oliver Eberta
School of Veterinary and Biomedical Sciences: Faculty Publications
Vesicular stomatitis virus (VSV), a negative-sense single-stranded-RNA rhabdovirus, is an extremely promising oncolytic agent for cancer treatment. Since oncolytic virotherapy is moving closer to clinical application, potentially synergistic combinations of oncolytic viruses and molecularly targeted antitumor agents are becoming a meaningful strategy for cancer treatment. Mitogenactivated protein kinase (MAPK) inhibitors have been shown to impair liver cell proliferation and tumor development, suggesting their potential use as therapeutic agents for hepatocellular carcinoma (HCC). In this work, we show that the impairment of MAPK in vitro did not interfere with the oncolytic properties of VSV in HCC cell lines. Moreover, the administration …
Identification Of Amino Acid Residues Important For Anti-Ifn Activity
Of Porcine Reproductive And Respiratory Syndrome Virus
Non-Structural Protein 1,
2012
University of Nebraska-Lincoln
Identification Of Amino Acid Residues Important For Anti-Ifn Activity Of Porcine Reproductive And Respiratory Syndrome Virus Non-Structural Protein 1, Lalit Beura, Sakthivel Subramaniam, Hiep Vu, Byungjoon Kwon, Asit K. Pattnaik, Fernando A. Osorio
School of Veterinary and Biomedical Sciences: Faculty Publications
The non-structural protein 1 (nsp1) of porcine reproductive and respiratory syndrome virus is partly responsible for inhibition of type I interferon (IFN) response by the infected host. By performing alanine-scanning mutagenesis, we have identified amino acid residues in nsp1α and nsp1β~ (the proteolytic products of nsp1) that when substituted with alanine(s) exhibited significant relief of IFNsuppression. A mutant virus (16-SA, in which residues 16-20 of nsp1β were substituted with alanines) encoding mutant nsp1β recovered from infectious cDNA clone was shown to be attenuated for growth in vitro and induced significantly higher amount of type I IFN transcripts in infected macrophages. …
Glycosyl-Phosphatidylinositol (Gpi)-Anchored Membrane Association Of The Porcine
Reproductive And Respiratory Syndrome Virus Gp4 Glycoprotein And Its Co-Localization
With Cd163 In Lipid Rafts,
2012
University of Illinois at Urbana-Champaign
Glycosyl-Phosphatidylinositol (Gpi)-Anchored Membrane Association Of The Porcine Reproductive And Respiratory Syndrome Virus Gp4 Glycoprotein And Its Co-Localization With Cd163 In Lipid Rafts, Yijun Du, Asit K. Pattnaik, Cheng Song, Dongwan Yoo, Gang Li
School of Veterinary and Biomedical Sciences: Faculty Publications
The porcine reproductive and respiratory syndrome virus (PRRSV) glycoprotein 4 (GP4) resembles a typical type I membrane protein in its structure but lacks a hydrophilic tail at the C-terminus, suggesting that GP4 may be a lipid-anchored membrane protein. Using the human decay-accelerating factor (DAF; CD55), a known glycosyl-phosphatidylinositol (GPI) lipid-anchored protein, chimeric constructs were made to substitute the GPI-anchor domain of DAF with the putative lipid-anchor domain of GP4, and their membrane association and lipase cleavage were determined in cells. The DAF-GP4 fusion protein was transported to the plasma membrane and was cleaved by phosphatidylinositol-specific phospholipase C (PI-PLC), indicating that …
A Single Amino Acid Change Resulting In Loss Of Fluorescence Of Egfp In A Viral
Fusion Protein Confers Fitness And Growth Advantage To The Recombinant
Vesicular Stomatitis Virus,
2012
University of Nebraska-Lincoln
A Single Amino Acid Change Resulting In Loss Of Fluorescence Of Egfp In A Viral Fusion Protein Confers Fitness And Growth Advantage To The Recombinant Vesicular Stomatitis Virus, Phat X. Dinh, Debasis Panda, Phani B. Das, Subash C. Das, Anshuman Das, Asit K. Pattnaik
School of Veterinary and Biomedical Sciences: Faculty Publications
Using a recombinant vesicular stomatitis virus encoding eGFP fused in-frame with an essential viral replication protein, the phosphoprotein P, we show that during passage in culture, the virus mutates the nucleotide C289 within eGFP of the fusion protein PeGFP to A or T, resulting in R97S/C amino acid substitution and loss of fluorescence. The resultant non-fluorescent virus exhibits increased fitness and growth advantage over its fluorescent counterpart. The growth advantage of the non-fluorescent virus appears to be due to increased transcription and replication activities of the PeGFP protein carrying the R97S/C substitution. Further, our results show that the R97S/C mutation …
Amino Acid Residues In The Non-Structural Protein 1 Of Porcine Reproductive
And Respiratory Syndrome Virus Involved In Down-Regulation Of Tnf-Cx
Expression In Vitro And Attenuation In Vivo,
2012
University of Nebraska-Lincoln
Amino Acid Residues In The Non-Structural Protein 1 Of Porcine Reproductive And Respiratory Syndrome Virus Involved In Down-Regulation Of Tnf-Cx Expression In Vitro And Attenuation In Vivo, Sakthivel Subramaniam, Lalit Beura, Byungjoon Kwon, Asit K. Pattnaik, Fernando A. Osorio
School of Veterinary and Biomedical Sciences: Faculty Publications
Porcine reproductive and respiratory syndrome virus (PRRSV) suppresses tumor necrosis factor-alpha (TNF-α) production at both transcriptional and post-transcriptional levels by its non-structural proteins 1α and 1β (Nsp1α and Nsp1β). To identifY the amino acid residues responsible for this activity, we generated several alanine substitution mutants of Nsp1α and Nsp1β. Examination of the mutant proteins revealed that Nsp1α residues Gly90, Asn91 , Arg97, Argl 00 and Arg124 were necessary for TNF-α promoter suppression, whereas several amino acids spanning the entire Nsp1β ~ were found to be required for this activity. Two mutant viruses, with mutations at Nsp1α Gly90 or Nsp1β residues …
Fusion Of A Fluorescent Protein To The Pul25
Minor Capsid Protein Of Pseudorabies Virus
Allows Live-Cell Capsid Imaging With Negligible
Impact On Infection,
2012
Northwestern University Feinberg School of Medicine
Fusion Of A Fluorescent Protein To The Pul25 Minor Capsid Protein Of Pseudorabies Virus Allows Live-Cell Capsid Imaging With Negligible Impact On Infection, Kevin P. Bohannon, Patricia J. Sollars, Gary E. Pickard, Gregory A. Smith
School of Veterinary and Biomedical Sciences: Faculty Publications
In order to resolve the location and activity of submicroscopic viruses in living cells, viral proteins are often fused to fluorescent proteins (FPs) and visualized by microscopy. In this study, we describe the fusion of FPs to three proteins of pseudorabies virus (PRV) that allowed imaging of capsids in living cells. Included in this study are the first recombinant PRV strains expressing FP–pUL25 fusions based on a design applied to herpes simplex virus type 1 by Homa and colleagues. The properties of each reporter virus were compared in both in vitro and in vivo infection models. PRV strains expressing FP–pUL25 …
Staphylococcal Response To Oxidative Stress,
2012
University of Nebraska - Lincoln
Staphylococcal Response To Oxidative Stress, Rosmarie Gaupp, Nagender Ledala, Greg A. Somerville
School of Veterinary and Biomedical Sciences: Faculty Publications
Staphylococci are a versatile genus of bacteria that are capable of causing acute and chronic infections in diverse host species. The success of staphylococci as pathogens is due in part to their ability to mitigate endogenous and exogenous oxidative and nitrosative stress. Endogenous oxidative stress is a consequence of life in an aerobic environment; whereas, exogenous osidative and nitrosative stress are often due to the bacteria's interaction with host immune systems. To overcome the deleterious effects of oxidative and nitrosative stress, staphylococci have evolved protection, detoxification, and repair mechanisms that are controlled by a network of regulators. In this review, …
Koutango: Under Reported Arboviral Disease In West Africa,
2012
Louisiana State University and Agricultural and Mechanical College
Koutango: Under Reported Arboviral Disease In West Africa, Jaime Matias De Araujo Lobo
LSU Doctoral Dissertations
Arthropod-borne viruses (arboviruses) are among the most common agents of human febrile illnesses worldwide. As crucially important emerging pathogens, they have caused multiple, notable epidemics of human disease and unnoticed epizootics over recent decades. Despite the public health relevance, very little is known about the geographic distribution of the agents and vectors, relative impact, and risk factors associated to the arboviral infection in many regions of the world and in the tropics in particular. Presented in this dissertation is an experimental study that explores the serology screening of serum samples from 151 patients whom were diagnosed with undifferentiated febrile illness …
Development And Characterization Of A Murine Model Of Rickettsia Parkeri Rickettsiosis,
2012
Louisiana State University and Agricultural and Mechanical College
Development And Characterization Of A Murine Model Of Rickettsia Parkeri Rickettsiosis, Britton Grasperge
LSU Doctoral Dissertations
Rickettsia parkeri, a member of the spotted fever group of Rickettsia, is the agent of an emerging rickettsiosis in the southeastern United States and South America. Despite increased recognition of human cases, limited information is available regarding infection of invertebrate and vertebrate hosts for this emerging tick-borne disease. Towards development of a viable transmission model and to further characterize the pathology associated with R. parkeri infection, inbred mouse strains (A/J, Balb/C, C3H/HeJ, and C3H/HeN) were intravenously and intradermally inoculated with R. parkeri. The C3H/HeJ strain of mice were identified as the most susceptible to R. parkeri infection and were found …
Fractures In Young, Single-Humped Camels (Camelus Dromedarius),
2012
TÜBİTAK
Fractures In Young, Single-Humped Camels (Camelus Dromedarius), Ahmed F. Ahmed, Fahd Abdullah Al-Sobayil
Turkish Journal of Veterinary & Animal Sciences
The objectives were to study the frequencies and classification of fractures in young camels and to evaluate the clinical relevance of external fixation as a method of treatment. Cases of fractures (n = 75) in young camels (less than 2 years old) were studied. On admission, the cause, site, classification, and radiography of the fractures as well as the methods of treatment were investigated. Factors affecting fracture healing after treatment were investigated and analyzed. The frequencies of fracture were affected by breed (P = 0.001) and age (P = 0.01) but not sex. Trauma was the most common cause of …
Serum Protein Fractions In Brazilian-Breed Donkeys Using Agarose Gel Electrophoresis,
2012
TÜBİTAK
Serum Protein Fractions In Brazilian-Breed Donkeys Using Agarose Gel Electrophoresis, Paulo Henrique Cavalcante, Andrea Cristina Silva, Sidnei Sakamoto, Benito Soto-Blanco
Turkish Journal of Veterinary & Animal Sciences
The values for the serum protein profiles of horses are available in many studies. However, only one study has characterized the electrophoretic migration profile of plasma protein in donkeys. The present study aimed to determine by agarose gel electrophoresis the normal values for serum protein fractions in erratic Brazilian-breed donkeys and to compare these values with those of the serum proteins of horses. Serum samples were collected from 26 donkeys of the Brazilian-breed and from 10 healthy horses. The serum levels of the total proteins were determined by the biuret method. The separation and determination of the values of serum …
Antioxidative And Antimicrobial Effects Of Garlic In Ground Camel Meat,
2012
TÜBİTAK
Antioxidative And Antimicrobial Effects Of Garlic In Ground Camel Meat, Hamid Reza Gheisari, Vahid Reza Ranjbar
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to investigate the antioxidative and antimicrobial effects of equivalent concentrations of garlic derivatives in ground camel meat during storage at 4 ± 1 °C. The addition of either garlic or butylated hydroxyanisole (BHA) (0.1 g/kg) significantly delayed lipid oxidation when compared with the control. The antioxidant activities of the various ingredients added followed the order of fresh garlic (FG), garlic powder (GP), BHA, and garlic oil (GO). After 14 days' storage, the aerobic plate count (APC) of both FG- and GP-formulated meat was significantly lower than that of either the control or BHA-formulated samples. …
Performance Analysis Of A Two Stage Pad Cooling System In Broiler Houses,
2012
TÜBİTAK
Performance Analysis Of A Two Stage Pad Cooling System In Broiler Houses, Meti̇n Petek, Serdal Di̇kmen, Mehmet Mustafa Oğan
Turkish Journal of Veterinary & Animal Sciences
The cooling performance of a traditional (control) and two stage evaporative pad cooling system (experiment), including pad and fan with tunnel ventilation, for poultry houses and the growth performance of broilers reared in these systems was compared in a study performed during extreme summer temperatures. The experimental unit had a two stage pad cooling system, which consisted of underground tubes and a box, followed by a pad cooling system with tunnel ventilation. The control unit had a traditional evaporative cooling system, including pad and fan with tunnel ventilation. Air temperature, relative humidity, and saturation efficiency were used to determine the …
A Determination Of Growth Hormone Receptor Gene Polymorphisms In East Anatolian Red Cattle, South Anatolian Red Cattle, And Turkish Grey Cattle, Iraz Akiş Akad, Ahmet Mengi̇, Kemal Özdem Öztabak
Turkish Journal of Veterinary & Animal Sciences
The objective of this study was to determinate the genotype and allele distributions of growth hormone receptor gene polymorphisms in East Anatolian Red cattle (EAR), South Anatolian Red cattle (SAR), and Turkish Grey (TG) cattle. To this end, 50 EAR, 50 SAR, and 50 TG cattle were used in the study. DNA samples were isolated by using the standard ammonium acetate salt-out method. Target regions were amplified and digested by AluI, AccI, StuI, NsiI, and Fnu4HI restriction enzymes. The + allele frequency of growth hormone receptor (GHR)/AluI polymorphism related with milk traits in TG cattle and the - allele frequencies …
Optimum Timing For Operation In Bitches With Pyometra Related To Endotoxemia,
2012
TÜBİTAK
Optimum Timing For Operation In Bitches With Pyometra Related To Endotoxemia, Meli̇h Uçmak, Çağatay Tek, Mehmet Can Gündüz, Ahmet Sabuncu, Adem Şenünver, Arzu Funda Bağcigi̇l, Tülay Bakirel
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to evaluate the plasma levels of endotoxin in bitches with pyometra and to investigate the effects of preoperative supportive therapy on plasma endotoxin levels (LPS), the complete blood count, serum biochemistry parameters (alanine amino transferase (ALT), alkaline phosphatase (ALP), total protein, albumin, urea, and creatinine), clinical health status at the 12th, 24th, and 48th h following presentation, and to explore if they were correlated. Randomly allocated into 2 equivalent groups were 20 bitches with pyometra, aged from 3 to 16 years (mean 9.15 ± 3.65 years). After collection of the samples, bitches in ovariohysterectomy …
