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Plasmid Profiles Of Multidrug-Resistant Escherichia Coli From Clinically Healthy Swine, Khin Khin Lay, Nisit Chansong, Rungtip Chuanchuen 2012 Chulalongkorn University

Plasmid Profiles Of Multidrug-Resistant Escherichia Coli From Clinically Healthy Swine, Khin Khin Lay, Nisit Chansong, Rungtip Chuanchuen

The Thai Journal of Veterinary Medicine

A total of 145 Escherichia coli isolates originated from clinically-healthy pigs were analyzed for plasmidprofiles. All but one isolate (n=144) carried one to nine plasmids with size ranging from 0.7-16.2 Mda. Fifty-sixplasmid profiles were defined based on sizes and numbers of plasmids in each strain, of which the most commonpatterns were 15.8, 4, 3.3, 1.7, 1.3 Mda and 16.2, 15.8, 4, 3.3, 2.3, 1.7, 1.3, 1, 0.7 Mda. The results indicate widedistribution of plasmids among the commensal E. coli isolates originated from clinically-healthy pigs.


Neospora Caninum And Toxoplasma Gondii Antibodies In Captive Elephants (Elephaus Maximus Indicus) In Kanchanaburi Province, Jitbanjong Wiengcharoen, Weerapan Nokkaew, Samart Prasithpon, Pornsak Prasomtong, Yaowalark Sukthana 2012 Chulalongkorn University

Neospora Caninum And Toxoplasma Gondii Antibodies In Captive Elephants (Elephaus Maximus Indicus) In Kanchanaburi Province, Jitbanjong Wiengcharoen, Weerapan Nokkaew, Samart Prasithpon, Pornsak Prasomtong, Yaowalark Sukthana

The Thai Journal of Veterinary Medicine

Although neosporosis has long been detected in several wildlife species from different parts of the world,until now there has been no report of Neospora caninum infection in elephants in any country of the world. Serumsamples of 115 captive elephant (Elephas maximus indicus) from the westernmost province of Kanchanaburi, Thailandwere investigated for antibodies to N. caninum and Toxoplasma gondii. Antibodies of N. caninum were detected by thecompetitive ELISA (cELISA) test and T.gondii by the Latex agglutination (LAT) test. The prevalence of T. gondiiantibodies was 13.04% (15/115), while anti-N. caninum was 33.04% (38/115). Only 7/115 (6.09%) were positive forboth parasites. Our study …


Ecg Quiz, Chollada Buranakarl, Kittipong Tachampa 2012 Chulalongkorn University

Ecg Quiz, Chollada Buranakarl, Kittipong Tachampa

The Thai Journal of Veterinary Medicine

No abstract provided.


Ophthalmology Snapshot, Nalinee Tuntivanich 2012 Chulalongkorn University

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.


Ultrasound Diagnosis, Phiwipha Kamonrat 2012 Chulalongkorn University

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.


What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep 2012 Chulalongkorn University

What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep

The Thai Journal of Veterinary Medicine

No abstract provided.


Molecular Characterization And Detection Of Babesia Canis Vogeli In Asymptomatic Roaming Dogs In Chiang Mai, Thailand, Kittisak Buddhachat, Orachon Meesong, Korakot Nganvongpanit, Maslin Osathanunkul, Siriwadee Chomdej 2012 Chulalongkorn University

Molecular Characterization And Detection Of Babesia Canis Vogeli In Asymptomatic Roaming Dogs In Chiang Mai, Thailand, Kittisak Buddhachat, Orachon Meesong, Korakot Nganvongpanit, Maslin Osathanunkul, Siriwadee Chomdej

The Thai Journal of Veterinary Medicine

The purpose of this study is to detect Babesia canis by using 18S rDNA amplification in order to confirm thepresence of the agents and to characterize molecularly the Thai B. canis. Three sets of primers, a Babesia canis-specificprimer (BcSP), Piroplasm-specific primer (PSP) and Babesia species-specific primer (BsSP), were tested for sensitivity.The results showed that BcSP and PSP were 50 times more sensitive than BsSP. Both BcSP and PSP were specificenough to detect this parasite in asymptomatic dogs. Peripheral blood samples were collected from 102 asymptomaticdogs residing in Chiang Mai and assayed with a light microscope and PCR by using BcSP …


South Dakota Animal Disease Research & Diagnostic Laboratory User's Guide, Animal Disease Research and Diagnostic Laboratory, South Dakota State University 2012 South Dakota State University

South Dakota Animal Disease Research & Diagnostic Laboratory User's Guide, Animal Disease Research And Diagnostic Laboratory, South Dakota State University

South Dakota Animal Disease Research and Diagnostic Laboratory (ADRDL)

The ADRDL is an accredited AAVLD laboratory and a member of the USDA National Animal Health Network. This document is user's guide for the services provided by the Animal Disease Research and Diagnostic Laboratory.


Raccoon Acclimation Towards Traps And Its Effect On Surveillance And Monitoring Of Zoonotic Diseases, Lindsay Rollend 2012 University of Connecticut

Raccoon Acclimation Towards Traps And Its Effect On Surveillance And Monitoring Of Zoonotic Diseases, Lindsay Rollend

Master's Theses

Raccoons (Procyon lotor) serve as a source of infection with various pathogens for people, most notably, rabies. Raccoons are specifically targeted for the control of rabies in the United States, primarily due to the 100% fatality rate in humans and animals. There is no cure or treatment for rabies and vaccines may be administered to people if it is administered promptly. In order to evaluate how successful trapping of raccoons is by using conventional methods, motion sensing cameras were placed on traps in order to captured raccoon behaviors. Pictures were coded based on these behaviors (around the trap but not …


Modeling Of The Efficacy And Effectiveness Of Escherichia Coli O157:H7 Pre-Harvest Interventions, Amanda R. Vogstad 2012 University of Nebraska-Lincoln

Modeling Of The Efficacy And Effectiveness Of Escherichia Coli O157:H7 Pre-Harvest Interventions, Amanda R. Vogstad

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Shiga toxin-producing Escherichia coli O157:H7 (STEC O157) is one of the leading causes of hemolytic uremic syndrome in humans. Reducing the prevalence of STEC O157 in live cattle may reduce ground beef prevalence and subsequent human illness. Type III secreted protein vaccines (TTSP) reduce fecal shedding of STEC O157 in cattle. However, pre-harvest vaccines have yet to be adopted by the beef industry. The objectives of this thesis were to 1) conduct a meta-analysis to test factors effecting efficacy of a 3-dose regimen TTSP vaccine product, and 2) use stochastic simulation to model the usefulness of preharvest control measures. In …


Cell-Mediated Immunity In Porcine Reproductive And Respiratory Syndrome Virus, Rajeshwari Parida 2012 University of Nebraska-Lincoln

Cell-Mediated Immunity In Porcine Reproductive And Respiratory Syndrome Virus, Rajeshwari Parida

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Porcine reproductive and respiratory syndrome virus is a significant swine pathogen which exhibits considerable sequence diversity. In an attempt to identify highly conserved T-cell epitopes contained in proteins of this virus, we examined heptadecamer peptides spanning the sequence of the PRRSV nonstructural proteins 9, 10 and 11, all of them are highly conserved, for their ability to elicit a recall proliferative and interferon-gamma response in peripheral blood mononuclear cells obtained from pigs immunized against the type-II PRRSV strain FL-12. These studies led to the identification of seven peptides, two from each NSP 9 and NSP 10 and, three from NSP …


Recombinant Protein Production In Bacillus Species, Isolation And Methionine Production In Methionine-Producing Microorganisms, Suwat Saengkerdsub 2012 University of Arkansas, Fayetteville

Recombinant Protein Production In Bacillus Species, Isolation And Methionine Production In Methionine-Producing Microorganisms, Suwat Saengkerdsub

Graduate Theses and Dissertations

Linoleic acid isomerase (LAI) is the enzyme that converts linoleic acid to conjugated linoleic acids (CLAs) which appear to be capable of lowering cancer risk and enhancing immunity. There is an interest in developing commercial processes for the production of single isomers of CLA by biotransformation of LA using microbial cells and enzymes. However, the evaluation of these strains suggest that growth and LA isomerase production levels by these anaerobes are insufficient to support economic commercial production of single CLA isomers. A better alternative is to clone the isomerase gene and to generate new production strains using recombinant technology. Bifidobacterium …


Effects Of Respiratory Vaccination Timing And Hormonal Growth Implant On Health, Performance, And Immunity Of Newly Received Stocker Calves, Kimberly Denise Poe 2012 University of Arkansas, Fayetteville

Effects Of Respiratory Vaccination Timing And Hormonal Growth Implant On Health, Performance, And Immunity Of Newly Received Stocker Calves, Kimberly Denise Poe

Graduate Theses and Dissertations

Stress alters the immune system and vaccination during this time may reduce vaccine response; whereas, growth implants may shift metabolism to enhance tissue deposition in exchange for energy required for immune response during bovine respiratory disease (BRD) challenge. This study was conducted to determine the effects of pentavalent respiratory vaccination timing with or without a hormonal growth implant on arrival (d 0) on health, performance, complete blood count, and vaccine response in high&ndashrisk, newly received stocker calves during a 42&ndashd receiving period. Crossbred bull and steer calves (n = 385) were weighed (initial BW = 202 ± 4.1 kg), stratified …


The Presence Of Alpha Interferon At The Time Of Infection Alters The Innate And Adaptive Immune Responses To Porcine Reproductive And Respiratory Syndrome Virus, Susan L. Brockman, Crystal A. Loving, Eric A. Nelson, Laura C. Miller, Tracy L. Nicholson, Karen B. Register, Marvin J. Grubman, Douglas E. Brough, Marcus E. Kehrli Jr 2012 USDA, Agricultural Research Service

The Presence Of Alpha Interferon At The Time Of Infection Alters The Innate And Adaptive Immune Responses To Porcine Reproductive And Respiratory Syndrome Virus, Susan L. Brockman, Crystal A. Loving, Eric A. Nelson, Laura C. Miller, Tracy L. Nicholson, Karen B. Register, Marvin J. Grubman, Douglas E. Brough, Marcus E. Kehrli Jr

Veterinary and Biomedical Sciences Faculty Publications

Porcine reproductive and respiratory syndrome (PRRS) is one of the most devastating and costly diseases to the swine industry worldwide. Overall, the adaptive immune response to PRRS virus (PRRSV) is weak, which results in delayed elimination of virus from the host and inferior vaccine protection. PRRSV has been shown to induce a meager alpha interferon (IFN-α) response, and we hypothesized that elevated IFN-α levels early in infection would shorten the induction time and increase elements of the adaptive immune response. To test this, we measured both antibody and cell-mediated immunity in pigs after the administration of a nonreplicating human adenovirus …


Growing South Dakota (Spring 2012), College of Agriculture &. Biological Sciences 2012 South Dakota State University

Growing South Dakota (Spring 2012), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Powerful Potential: Growing Global Population Spurs Need for Agbioscience
[Page] 4 Spring College News
[Page] 6 Enhanced Education: Students Complement Coursework With Extra-Curricular Opportunities
[Page] 8 Gaining Global Perspective: SDSU Delegation Visits Argentina
[Page] 9 Creative Collaboration: Students, Landowners & Industry Professionals Pair Up for a Positive Experience
[Page] 10 Wondrous Wheat: New Research Agreement & Wheat Varieties Announced
[Page] 12 Here and There
[Page] 14 New Era Underway: SDSU Extension Re-Energizes Programs, Delivery [Page] 16 iGrow Continues to Grow
[Page] 18 Focused on the Future: Field Specialists Strive to Serve Changing Landowner Needs
[Page] 19 Connecting with …


The Effect Of Neutering On The Risk Of Urinary Incontinence In Bitches – A Systematic Review, W. Beauvais, J. M. Cardwell, D. C. Brodbelt 2012 Royal Veterinary College

The Effect Of Neutering On The Risk Of Urinary Incontinence In Bitches – A Systematic Review, W. Beauvais, J. M. Cardwell, D. C. Brodbelt

Endocrinology Collection

An increased risk of urinary incontinence in bitches has often been associated with previous ovariohysterectomy but remains controversial. The objective of this study was to evaluate the strength of evidence for an association between neutering or age at neutering and urinary incontinence in bitches and to estimate the magnitude of any effect found. A systematic review of peer‐reviewed original English analytic journal articles was conducted, based on Cochrane guidelines (Higgins and Green 2009) Of 1,853 records screened, seven studies were identified that examined the effect of neutering or age at neutering on the risk of urinary incontinence but …


Inflammatory Responses To Induced Infectious Endometritis In Mares Resistant Or Susceptible To Persistent Endometritis, Mette Christoffersen, Elizabeth Woodward, Anders M. Bojesen, Stine Jacobsen, Morten R. Petersen, Mats H. T. Troedsson, Henrik Lehn-Jensen 2012 University of Copenhagen, Denmark

Inflammatory Responses To Induced Infectious Endometritis In Mares Resistant Or Susceptible To Persistent Endometritis, Mette Christoffersen, Elizabeth Woodward, Anders M. Bojesen, Stine Jacobsen, Morten R. Petersen, Mats H. T. Troedsson, Henrik Lehn-Jensen

Veterinary Science Faculty Publications

BACKGROUND: The objective of the study was to evaluate the gene expression of inflammatory cytokines (interleukin [IL]-1β, IL-6, IL-8, IL-10, tumor necrosis factor [TNF]-α, IL-1 receptor antagonist [ra] and serum amyloid A (SAA) in endometrial tissue and circulating leukocytes in response to uterine inoculation of 105 colony forming units (CFU) Escherichia coli in mares. Before inoculation, mares were classified as resistant or susceptible to persistent endometritis based on their uterine inflammatory response to infusion of 109 killed spermatozoa and histological assessment of the endometrial quality. Endometrial biopsies were obtained 3, 12, 24 and 72 hours (h) after bacterial inoculation and …


Genetically Engineered Equine Influenza Virus And Uses Thereof, Peter Palese, Adolfo Garcia-Sastre, Thomas Chambers 2012 University of Kentucky

Genetically Engineered Equine Influenza Virus And Uses Thereof, Peter Palese, Adolfo Garcia-Sastre, Thomas Chambers

Veterinary Science Faculty Patents

The present invention relates, in general, to attenuated equine influenza viruses having an impaired ability to antagonize the cellular interferon (IFN) response, and the use of such attenuated viruses in vaccine and pharmaceutical formulations. In particular, the invention relates to attenuated equine influenza viruses having modifications to an equine NS1 gene that diminish or eliminate the ability of the NS1 gene product to antagonize the cellular IFN response. These viruses replicate in vivo, but demonstrate decreased replication, virulence and increased attenuation, and therefore are well suited for use in live virus vaccines, and pharmaceutical formulations.


Impact Of Preexisting Adenovirus Vector Immunity On Immunogenicity And Protection Conferred With An Adenovirus-Based H5n1 Influenza Vaccine, Aseem Pandey, Neetu Singh, Sai V. Vemula, Laurent Couetil, Jacqueline M. Katz, Ruben Donis, Suryaprakash Sambhara, Suresh K. Mittal 2012 Purdue University

Impact Of Preexisting Adenovirus Vector Immunity On Immunogenicity And Protection Conferred With An Adenovirus-Based H5n1 Influenza Vaccine, Aseem Pandey, Neetu Singh, Sai V. Vemula, Laurent Couetil, Jacqueline M. Katz, Ruben Donis, Suryaprakash Sambhara, Suresh K. Mittal

Department of Comparative Pathobiology Faculty Publications

The prevalence of preexisting immunity to adenoviruses in the majority of the human population might adversely impact the development of adaptive immune responses against adenovirus vector-based vaccines. To address this issue, we primed BALB/c mice either intranasally (i.n.) or intramuscularly (i.m.) with varying doses of wild type (WT) human adenovirus subtype 5 (HAd5). Following the development of immunity against HAd5, we immunized animals via the i.n. or i.m. route of inoculation with a HAd vector (HAd-HA-NP) expressing the hemagglutinin (HA) and nucleoprotein (NP) of A/Vietnam/1203/ 04 (H5N1) influenza virus. The immunogenicity and protection results suggest that low levels of vector …


In Vitro Efficacy Of Red Kwao Krua (Butea Superba Roxb.) Extract Against Streptococcal Bacteria Isolated From Diseased Tilapia (Oreochromis Niloticus), Nopadon Pirarat, Channarong Rodkhum, Aranya Ponpornpisit, Wanwipa Suthikrai 2012 Chulalongkorn University

In Vitro Efficacy Of Red Kwao Krua (Butea Superba Roxb.) Extract Against Streptococcal Bacteria Isolated From Diseased Tilapia (Oreochromis Niloticus), Nopadon Pirarat, Channarong Rodkhum, Aranya Ponpornpisit, Wanwipa Suthikrai

The Thai Journal of Veterinary Medicine

Streptococcal infection is a major problem in intensive tilapia farming. This study was conducted to investigate the antimicrobial activity of mucilage, distilled water- and 70% methanol-crude extract from Butea superba Roxb. against Streptococcal bacteria. The antimicrobial activity using disc diffusion assay showed that B. superba Roxb. had a broad antimicrobial range against twelve isolates of streptococcal bacteria: Streptococcus iniae (n=2) and Streptococcus agalactiae (n=10). The minimum inhibitory concentration (MIC) of the 70% methanol-crude extract varied between 128 and 512 µg/ml depending on the tested streptococcal strains. Mucilage has a higher potency in the inhibition of streptococcal bacteria when compared with …


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