Genetically Engineered Equine Influenza Virus And Uses Thereof,
2012
University of Kentucky
Genetically Engineered Equine Influenza Virus And Uses Thereof, Peter Palese, Adolfo Garcia-Sastre, Thomas Chambers
Veterinary Science Faculty Patents
The present invention relates, in general, to attenuated equine influenza viruses having an impaired ability to antagonize the cellular interferon (IFN) response, and the use of such attenuated viruses in vaccine and pharmaceutical formulations. In particular, the invention relates to attenuated equine influenza viruses having modifications to an equine NS1 gene that diminish or eliminate the ability of the NS1 gene product to antagonize the cellular IFN response. These viruses replicate in vivo, but demonstrate decreased replication, virulence and increased attenuation, and therefore are well suited for use in live virus vaccines, and pharmaceutical formulations.
Impact Of Preexisting Adenovirus Vector Immunity On Immunogenicity And Protection Conferred With An Adenovirus-Based H5n1 Influenza Vaccine,
2012
Purdue University
Impact Of Preexisting Adenovirus Vector Immunity On Immunogenicity And Protection Conferred With An Adenovirus-Based H5n1 Influenza Vaccine, Aseem Pandey, Neetu Singh, Sai V. Vemula, Laurent Couetil, Jacqueline M. Katz, Ruben Donis, Suryaprakash Sambhara, Suresh K. Mittal
Department of Comparative Pathobiology Faculty Publications
The prevalence of preexisting immunity to adenoviruses in the majority of the human population might adversely impact the development of adaptive immune responses against adenovirus vector-based vaccines. To address this issue, we primed BALB/c mice either intranasally (i.n.) or intramuscularly (i.m.) with varying doses of wild type (WT) human adenovirus subtype 5 (HAd5). Following the development of immunity against HAd5, we immunized animals via the i.n. or i.m. route of inoculation with a HAd vector (HAd-HA-NP) expressing the hemagglutinin (HA) and nucleoprotein (NP) of A/Vietnam/1203/ 04 (H5N1) influenza virus. The immunogenicity and protection results suggest that low levels of vector …
Causes Of Relinquishing The Dogs Adopted From Animal Shelters In Taiwan,
2012
Chulalongkorn University
Causes Of Relinquishing The Dogs Adopted From Animal Shelters In Taiwan, Ying-Bin Fuh, Meng-Chih Tung, Kwong-Chung Tung, Jeng-Tung Chiang, Chang-Young Fei
The Thai Journal of Veterinary Medicine
This study investigated the causes of relinquishing the dogs adopted from animal shelters. The subjects were the adopters of public animal shelters in Taiwan in 2003~2005. Random sampling was conducted upon 10% of the population. This study collected data by telephone survey, and 1,185 calls were made, with 626 valid samples. According to this investigation, when being adopted, most of the animals were below one year old (71.7%; 449/626); among 626 dogs investigated, 229 were relinquished (36.6%; 229/626). They were mostly relinquished within half year after the adoption (45.4%; 104/229). The main cause of abandonment is “behavior” (31.9%; 73/229); “barking” …
Ecg Quiz,
2012
Chulalongkorn University
Ecg Quiz, Chollada Buranakarl, Sirilak Disatian, Suwanakiat Sawangkoon, Winai Chansaisakorn
The Thai Journal of Veterinary Medicine
No abstract provided.
Ophthalmology Snapshot,
2012
Chulalongkorn University
Ophthalmology Snapshot, Nalinee Tuntivanich
The Thai Journal of Veterinary Medicine
No abstract provided.
Serotype, Antimicrobial Susceptibility, And Genotype Of Salmonella Isolates From Swine And Pork In Sa Kaew Province, Thailand,
2012
Chulalongkorn University
Serotype, Antimicrobial Susceptibility, And Genotype Of Salmonella Isolates From Swine And Pork In Sa Kaew Province, Thailand, Chaiwat Pulsrikarn, Phattharaphron Chaichana, Srirat Pornruangwong, Yukio Morita, Shigeki Yamamoto, Sumalee Boonmar
The Thai Journal of Veterinary Medicine
In January 2010, we examined the prevalence of Salmonella in the rectal swab of 66 swine on 8 farms and in 25 pork products at 6 meat retailers of Sa Kaew province. Salmonella was isolated from 3%(2/66) of swine rectal swab samples and 96%(24/25) of pork samples. In rectal swab samples, both S. Weltevreden and S. Dumfries were isolated from one sample, and S. Stanley from another sample. In pork, a total of 42 isolates (16 serovars) were found ; 8 strains of S. Rissen, 5 strains of S. Stanley and S. Anatum, 4 strains of S. Give and S. …
In Vitro Efficacy Of Red Kwao Krua (Butea Superba Roxb.) Extract Against Streptococcal Bacteria Isolated From Diseased Tilapia (Oreochromis Niloticus),
2012
Chulalongkorn University
In Vitro Efficacy Of Red Kwao Krua (Butea Superba Roxb.) Extract Against Streptococcal Bacteria Isolated From Diseased Tilapia (Oreochromis Niloticus), Nopadon Pirarat, Channarong Rodkhum, Aranya Ponpornpisit, Wanwipa Suthikrai
The Thai Journal of Veterinary Medicine
Streptococcal infection is a major problem in intensive tilapia farming. This study was conducted to investigate the antimicrobial activity of mucilage, distilled water- and 70% methanol-crude extract from Butea superba Roxb. against Streptococcal bacteria. The antimicrobial activity using disc diffusion assay showed that B. superba Roxb. had a broad antimicrobial range against twelve isolates of streptococcal bacteria: Streptococcus iniae (n=2) and Streptococcus agalactiae (n=10). The minimum inhibitory concentration (MIC) of the 70% methanol-crude extract varied between 128 and 512 µg/ml depending on the tested streptococcal strains. Mucilage has a higher potency in the inhibition of streptococcal bacteria when compared with …
Treatment Of Canine Transmissible Venereal Tumor Using Vincristine Sulfate Combined With L-Asparaginase In Clinical Vincristine-Resistant Cases: A Case Report,
2012
Chulalongkorn University
Treatment Of Canine Transmissible Venereal Tumor Using Vincristine Sulfate Combined With L-Asparaginase In Clinical Vincristine-Resistant Cases: A Case Report, Pansawut Pansawut, Patrakrit Theewasutrakul, Sirichai Techarungchaikul, Suppawiwat Ponglowhapan, Kaywalee Chatdarong
The Thai Journal of Veterinary Medicine
Three female mongrel dogs were cytologically diagnosed as transmissible venereal tumor (TVT) and had clinically developed resistance to vincristine treatment. One dog was treated with four treatments of 10,000 IU/m2 body surface area of L-asparaginase combined with 0.025 mg/kg body weight of vincristine sulfate every two weeks while this combination was administered to the others once a week for four weeks and only vincristine sulfate once a week for four treatments. Both treatments resulted in a complete remission. Side effects such as gastrointestinal upset, diarrhea, depression, and decrease in appetite were observed in the dogs administered with the later protocol. …
Ultrasound Diagnosis,
2012
Chulalongkorn University
Ultrasound Diagnosis, Phiwipha Kamonrat
The Thai Journal of Veterinary Medicine
No abstract provided.
Detection And Segregation Of Brucella Abortus And Brucella Melitensis In Aborted Bovine, Ovine, Caprine, Buffaloes And Camelid Fetuses By Application Of Conventional And Real-Time Polymerase Chain Reaction,
2012
Chulalongkorn University
Detection And Segregation Of Brucella Abortus And Brucella Melitensis In Aborted Bovine, Ovine, Caprine, Buffaloes And Camelid Fetuses By Application Of Conventional And Real-Time Polymerase Chain Reaction, Farhad Safarpoor Dehkordi, Shahin Saberian, Hassan Momtaz
The Thai Journal of Veterinary Medicine
This present study was carried out to use conventional and real-time PCR for detection and segregation of Brucella abortus and Brucella melitensis in aborted bovine, ovine, caprine, buffalo and camel fetuses. All samples were collected and immediately transferred to laboratory, genomic DNA was extracted and the conventional and real-time PCR by specific primers for Brucella abortus and Brucella melitensis was performed. A TaqMan analysis and single-step PCR was carried out in total 3710 DNA of abomasal contents of aborted fetuses. In total, 281/892 (31.5%) bovine, 224/810 (27.65%) ovine, 219/786 (27.86%) caprine, 199/604 (32.94%) buffalo and 201/618 (32.52%) camel fetus samples …
Rt-Pcr Survey Of Emerging Paramyxoviruses In Cave-Dwelling Bats,
2012
Chulalongkorn University
Rt-Pcr Survey Of Emerging Paramyxoviruses In Cave-Dwelling Bats, Lerdchai Chintapitasakul, Nimit Choengern, Sara Bumrungsri, Mutita Chalamat, Lamul Molee, Sujira Parchariyanon, Somchuan Ratanamungklanon, Kritaya Kongsuwan
The Thai Journal of Veterinary Medicine
Bats are the reservoir hosts for several Paramyxoviruses including two serious zoonotic viruses, Hendra virus and Nipah virus which are responsible for fatal infections in animals and humans. These two viruses are sufficiently different from previously described Paramyxoviruses and are included in a new genus, Henipavirus. We report here a survey of cave-dwelling, insectivorous bats in Thailand for the presence of henipaviruses. Pooled urine samples were collected in nine caves inhabited by six different bat species in the northern (Chiangmai and Nakornsawan) and southern (Songkla and Satoon) provinces of Thailand. A reverse transcription PCR (RT-PCR) assay using henipavirus-specific primers derived …
Mosquito Distribution And Japanese Encephalitis Virus Infection In A Bat Cave And Its Surrounding Area In Lopburi Province, Central Thailand,
2012
Chulalongkorn University
Mosquito Distribution And Japanese Encephalitis Virus Infection In A Bat Cave And Its Surrounding Area In Lopburi Province, Central Thailand, Sonthaya Tiawsirisup, Alisa Junpee, Surang Nuchprayoon
The Thai Journal of Veterinary Medicine
Mosquito distribution and Japanese encephalitis virus (JEV) infection were studied in a bat cave and its surrounding area in Lopburi province, central Thailand. Mosquitoes were collected from May 2009 to April 2010 by using CO2-baited CDC-light traps, and dry ice as a source of CO2 to attract mosquitoes. Mosquitoes were identified and tested for JEV infection by using reverse transcription polymerase chain reaction (RT-PCR). Five genera of mosquitoes collected from the bat cave include Aedes, Anopheles, Armigeres, Culex and Uranotaenia, and eight genera of mosquitoes collected from the area close to the bat cave include Aedes, Aedeomyia, Anopheles, Armigeres, Coquillettidia, …
The Pathogenesis Of A New Variant Genotype And Qx-Like Infectious Bronchitis Virus Isolated From Chickens In Thailand,
2012
Chulalongkorn University
The Pathogenesis Of A New Variant Genotype And Qx-Like Infectious Bronchitis Virus Isolated From Chickens In Thailand, Tawatchai Pohuang, Jiroj Sasipreeyajan
The Thai Journal of Veterinary Medicine
This study was designed to investigate the pathogenesis of infectious bronchitis virus (IBV), a new variantgenotype and QX-like IBV, isolated in Thailand. Sixty, 28-day-old, female broiler chickens were divided into 3 groupsof 20 chickens each (groups 1-3) and sixty, 1-day-old, female broiler chickens were divided into 3 groups of 20chickens each (groups 4-6). The chickens in groups 2 and 5 were inoculated with QX-like IBV (isolate THA80151)whereas the chickens in groups 3 and 6 were inoculated with a new variant genotype IBV (isolate THA90151). Thechickens in groups 1 and 4 served as negative control groups. Clinical signs and mortality rates …
Time Of The First Embryonic Cleavage Indicates Cat Blastocyst Quality,
2012
Chulalongkorn University
Time Of The First Embryonic Cleavage Indicates Cat Blastocyst Quality, Nuttha Klincumhom, Ampika Thongphakdee, Mongkol Techakumphu, Kaywalee Chatdarong
The Thai Journal of Veterinary Medicine
Time of the first cleavage has been used as an indicator to determine developmental rate and quality of embryos in many species. The aim of this study was to examine effect of time of the first cleavage on cat embryo development and blastocyst quality. After in vitro fertilization, embryos were observed twice at 27 and 42 hours. Embryos cleaved at 27 hours post-fertilization (pf) were classified as early cleaved embryos and were separated from the remainder which were determined as delayed cleaved embryos (cleaved between 27 and 42 hours). These two groups were separately cultured in the same condition for …
Efficacy Of Different Vaccination Programs Against Thai Qx-Like Infectious Bronchitis Virus,
2012
Chulalongkorn University
Efficacy Of Different Vaccination Programs Against Thai Qx-Like Infectious Bronchitis Virus, Jiroj Sasipreeyajan, Tawatchai Pohuang, Nida Sirikobkul
The Thai Journal of Veterinary Medicine
The present study was designed to evaluate the protection induced by different vaccination programs against Thai QX-like infectious bronchitis virus (IBV). The chickens were divided into 5 groups, 16 chickens in each. Groups 1-4 were vaccinated with different regimes of vaccination program against IBV. Groups 5 did not receive IBV vaccine and served as a positive control group. At 1 day old, the chickens in groups 2 and 3 were vaccinated with QX vaccine whereas the chickens in group 4 were vaccinated with H120 vaccine. At 14 days old, revaccination was performed in group 2 with QX vaccine whereas groups …
Reproductive And Dairy Performances Of Thai Swamp Buffaloes Under Intensive Farm Management,
2012
Chulalongkorn University
Reproductive And Dairy Performances Of Thai Swamp Buffaloes Under Intensive Farm Management, Thuchadaporn Chaikhun, Ranchuan Hengtrakunsin, Fabio De Rensis, Mongkol Techakumphu, Siriwat Suadsong
The Thai Journal of Veterinary Medicine
In this study the reproductive and dairy performances of swamp buffaloes under intensive farm conditions were evaluated. The data of reproduction and milk production were collected from forty-eight swamp buffaloes during the period of 2001 to 2010. The reproductive and dairy performances were analyzed as mean±SD of each index. The age at first calving was 47.1±8 months and the gestation period was 321.4±11.3 days. The service per conception was 3.5±2.5, with insemination by both natural and artificial mating. The calving interval and the days open were 486.2±75.02 and 166.2±69.7 days (n=99), respectively. Additionally, there was a large increase in primiparous …
Growth And Survival Of Brown Shrimp (Farfantepenaeus Aztecus) In A Closed Recirculation Seawater System At Different Salinities,
2012
Chulalongkorn University
Growth And Survival Of Brown Shrimp (Farfantepenaeus Aztecus) In A Closed Recirculation Seawater System At Different Salinities, Roberto Pérez-Castañeda, Jesús Genaro Sánchez-Martínez, Gabriel Aguirre-Guzmán
The Thai Journal of Veterinary Medicine
Growth and survival of juvenile brown shrimp (Farfantepenaeus aztecus) in a closed recirculation seawater system at two salinities (33 and 38‰) were evaluated during 4 weeks in experimental tanks. Although the specific growth rate (SGR) was slightly higher at the treatment of 38‰ than at 33‰, no significant differences were detected for the different time intervals analyzed; however, the growth in terms of both body weight and total length was significantly faster at 38‰. In this regard, the increase in weight and total length obtained at 38‰ were 0.33 g/week and 2.15 mm/week, respectively, whereas at 33‰ were 0.21 g/week …
What Is Your Diagnosis,
2012
Chulalongkorn University
What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep
The Thai Journal of Veterinary Medicine
No abstract provided.
Determination Of Antibiotic Resistance Prototype And In Vitro R Plasmid Conjugation Of Escherichia Coli Isolates From Broiler Feces,
2012
Chulalongkorn University
Determination Of Antibiotic Resistance Prototype And In Vitro R Plasmid Conjugation Of Escherichia Coli Isolates From Broiler Feces, Adnan Amin, Muhammad Ayaz Khan, Uzma Haroon, Sheikh Muhammad Farooq Azam, Saadia Andaleeb, Pir Bux Ghumro
The Thai Journal of Veterinary Medicine
The objective of the present investigation was to ascertain the prevalence of antibiotic resistance amongst E. coli strains obtained from broiler chicken in two different poultry farms against antibiotics that have a limited or no use in poultry and to advocate a probable mechanism of antibiotic resistance transfer between strains. A high resistance rate was seen against azteronam (100%), streptomycin (88-92%) followed by amoxicillin (68-76%), ceftrioxone (60-68%) and cefepime (56-68%). However reasonably lower levels of resistance were seen towards coamoxiclave (40%), ciprofloxacin (24-28%), and ofloxacin (36-40%). Meropenem emerged as least resistant (16-28%) antimicrobial agent. Likewise, virtually entire sum of isolates …
Effects Of Behavior Responses On The Vasovagal Tonus Index In Healthy Dogs,
2012
Chulalongkorn University
Effects Of Behavior Responses On The Vasovagal Tonus Index In Healthy Dogs, Walasinee Moonarmart, Kripitch Suttummaporn, Thapana Jarutummsiri, Rungrote Osathanoniri
The Thai Journal of Veterinary Medicine
The vasovagal tonus index (VVTI) is a time-domain analysis method of heart rate variability acquired over a short period. It is a useful measurement for evaluating severity and prognosis heart failure in dogs. Behavior responses can be used to evaluate stress in each dog individually. Stress during clinical examination may interfere with the VVTI since it influences the sympathetic nervous system. The aim of this study was to investigate the effect of behavior responses during clinical examination on the VVTI. Data set obtained from physical examination, systolic blood pressure measurement, electrocardiography, VVTI calculation, and video recording were collected from 50 …
