Veterinary Medicine Commons^™

Experimental Infection Of Mice And Baby Chickens With Thailand Strain Of Chikungunya Virus, Sonthaya Tiawsirisup, Pichanan Rattanakampol, Warritar Navavichit, Hemmarat Ratpiyapaporn

The Thai Journal of Veterinary Medicine

This study was conducted to investigate Chikungunya virus (CHIKV) infection in mammal and avian byusing mice and baby chickens as model animals. Thailand 2010 and Ross/186 strain (reference strain) were used inthis study. 104, 106 and 108 CID50 of CHIKV were inoculated to four- and six-week-old mice. Blood was collected andtested for virus for seven days. 108 CID50 of CHIKV was inoculated to two-week-old mice. Blood was collected andtested for virus for five days. For the baby chickens, 102, 104, 106 and 108 CID50 of CHIKV were inoculated to five-dayoldbaby chickens. Blood was collected and tested for virus for seven …

Distribution Of Spermatozoa In The Reproductive Tracts Of Sows After Intra-Uterine Insemination Using Frozen-Thawed Boar Semen, Panida Chanapiwat, Kampon Kaeoket, Padet Tummaruk

The Thai Journal of Veterinary Medicine

The aims of the present study were to determine the number of spermatozoa in the reproductive tract ofsows after intra-uterine insemination (IUI) with frozen-thawed (FT) boar semen and to investigate the influence ofadding seminal plasma in the thawing medium on the sperm transport in the female reproductive tract. Fourteenmultiparous sows were divided into 3 groups: group I (n= 5), insemination with 80 ml of extended fresh semen,group II (n= 4), insemination with 40 ml of FT semen diluted with ModenaTM extender and group III (n= 5),insemination with 40 ml of FT semen diluted with combination of ModenaTM and seminal plasma. …

Horner's Syndrome Associated With Escherichia Coli Infection In A Raccoon (Procyon Lotor) - A Case Report, Fernando Nájera Muñoz, Alfredo Carlos Suárez Saavedra

The Thai Journal of Veterinary Medicine

This clinical case describes a three-year-old male raccoon (Procyon lotor), castrated, from La Lajita Oasis ParkZoological Park (Spain) which showed lack of appetite, state of mental depression and mild dehydration. Duringclinical examination an abscess was detected in the left ventrolateral area of the neck as well as clinical signscompatible with Horner’s Syndrome in the left eye. A hypersensitivity test was carried out by sympatheticinnervation with topical epinephrine 0.0001% and results suggested the presence of second-order Horner’s Syndromeformed by pressure from the abscess on the sympathetic innervation of the left eye. A radiologic study of the neckwas performed and blood and …

Preservation Of Sudan Red Staining Of Brain After Plastination Process, Punnarat Vibulchan, Jantima Intarapanya, Ornsiri Cheunsuang

The Thai Journal of Veterinary Medicine

Specimen preservation is an essential factor in gross anatomical study of the brain and spinal cord. Forcedimpregnation of the specimen using polymer, or plastination, is to date one of the best specimen preparationprocedures. This study aimed to study the possibility of staining whole brain specimen using Sudan Red III dye priorto plastination. Dog brains were divided into 3 groups each stained with the dye for 1, 2 and 3 months, respectively.The stained specimens were then dissected to reveal the staining of deep structures before undergoing theplastination procedure. After the experiment we found that Sudan Red clearly stained the fiber tracts …

Surgical Treatment Of Renal Gout In Monocellate Cobra, Naja Kaouthia, Taksa Vasaruchapong, Lawan Chanhome

The Thai Journal of Veterinary Medicine

A wild captured, male Monocellate cobra, Naja kaouthia of unknown age was observed with anorexia, imbalance of swimming and mild swelling in the distal fourth of the body. Physical examination revealed a palpable firm mass in the coelomic cavity, of which radiographic examination was suspicious as an obstruction of large intestine by a foreign body. Removal of the foreign body through its cloaca was attempted by enema and digital manipulation, but was not successful. Thereafter, an exploratory surgery was performed and revealed an abnormality in the left kidney that was compressing the large intestine. Nephrectomy was performed and histopathologically identified …

Combination Of Acupuncture And Aquapuncture Using Vitamin B Complex For Treatment Of Chronic Degenerative Changes Of Hip Joints And Anal Relaxation In A Dog, Kui-Ming Chang, Kuan-Sheng Chen, Hsien-Chi Wang, Cheng-Hung Lai, Yu-Ta Hsieh, Hsing-Hui King, Wei-Ming Lee

The Thai Journal of Veterinary Medicine

A beagle dog, aged 10 years old, was presented with anal relaxation. Chronic arthrosis of hip joints had beendiagnosed and treated on this dog’s first visit to the National Chung Hsing University Veterinary Medicine Teachinghospital. Except for unwillingness to walk on both hind limbs, pain on both hips and uncontrolled defecation due toanal relaxation, no other clinical signs were found during physical examination. The body temperature was 38.60C.CRT was normal (less than 2 sec). Heart beat was 110 beats/min and strong pulse was palpated. Based on theGlasgow composite pain scale, the pain scale of this dog was 2. Radiology of …

Ecg Quiz, Chollada Buranakarl, Suwanakiat Sawangkoon, Monkon Trisiriroj, Winai Chansaisakorn

The Thai Journal of Veterinary Medicine

No abstract provided.

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep

The Thai Journal of Veterinary Medicine

No abstract provided.

Differential Expression Patterns Of Proteins Involved In Epidermal Proliferation And Differentiation In Canine Atopic Dermatitis, Sirin Theerawatanasirikul, Achariya Sailasuta, Roongroje Thanawongnuwech, Tossaporn Nakbed, Komkrid Charngkaew, Gunnaporn Suriyaphol

The Thai Journal of Veterinary Medicine

The pathological alterations in skin keratinocyte proliferation and differentiation in canine atopic dermatitis(CAD) were investigated in 20 small breed dogs with CAD (10 of which displayed lesional CAD skin and 10 nonlesionalCAD skin) and 11 healthy control animals. Biopsy of lesional CAD skin showed orthokeratotichyperkeratosis. Immunohistochemistry (IHC) of Ki-67, a cellular marker for proliferation, showed a higher epidermalcell proliferation rate in the CAD dogs (p< 0.05) which positively correlated with Canine Atopic Dermatitis Extent andSeverity Index, CADESI-03 (p< 0.01). IHC labeling indicated reduced expression of cornified envelope proteins,involucrin (IVL) and filaggrin (FLG) in both lesional and non-lesional skin (p< 0.05). In contrast, expression oflympho-epithelial Kazal-type inhibitor (LEKTI), which has been reported to inhibit proteases that cleave proFLG intoFLG, was increased in lesional skin compared to the normal controls (p< 0.05). The IHC results showed a positivecorrelation between CADESI-03 and LEKTI expression and a negative correlation between CADESI-03 and IVLexpression. In conclusion, the present study demonstrates that epidermal hyperkeratosis in CAD is related todisturbance of both epidermal proliferation and cornified envelope differentiation. This is the first report of acorrelation of LEKTI with CAD.

Molecular Analysis Of Medically And Veterinary Important Muscid Flies (Diptera: Muscidae) In Thailand, Payu Bhakdeenuan, Padet Siriyasatien, Sunchai Payungporn, Kanok Preativatanyou, Usavadee Thavara, Apiwat Tawatsin, Kom Sukontason, Kabkaew Likitvong Sukontason, Wej Choochote, Suttida Suwannayod, Hitoshi Sasaki

The Thai Journal of Veterinary Medicine

We demonstrated the using of the internal transcribed spacer (ITS2) of ribosomal DNA as a tool for identification of medically and veterinary important Muscidae flies in Thailand. A total of 27 fly samples were collected from various regions of Thailand. Six fly species in three subfamilies including Azeliinae (Hydrotaea spinigera), Muscinae (Musca domestica, M. sorbens) and Stomoxyinae (Stomoxys calcitrans, S. indicus and S. sitiens) were identified base on morphological taxonomy. PCR amplicons of the ITS2 gene of these flies varied between 312-377 bp with A+T content of 76.6%. ITS2 sequences of the flies in this study were 93-100% identity to …

Side Effects In 412 Dogs From Swimming In A Chlorinated Swimming Pool, Korakot Nganvongpanit, Terdsak Yano

The Thai Journal of Veterinary Medicine

This study reports the side effects in dogs from swimming in a chlorinated swimming pool. Data werecollected from September 2008 until April 2012. A total of 412 dogs (male= 219 and female= 193), aged 38±30 months,served as the subjects. The dogs were divided into four groups based on the aims of swimming: entertainment (n=152), weight reduction (n= 53), rehabilitation after orthopedic surgery (n= 81), and rehabilitation for muscle and jointdisease (n= 126). The data were recorded for five separate swimming times. At first, some dogs (29.13%) showedoverexcitement and/or fear, but this percentage decreased with increased swimming frequency. Some dogs (36.51%)were …

Apitoxin Aquapuncture And Administration Of Herbal Medicines In A Dog With Superficial Pyoderma, Cheng-Hung Lai, Pan-Chen Liu, Han-Yuan Lin

The Thai Journal of Veterinary Medicine

A 6-month-old, 7.2 kg, intact female mongrel stray dog wasreferred to the Veterinary Medical TeachingHospital, National Chung Hsing University with a chief complaint of alopecia, and severe pruritus. A skin swab wasobtained for bacterial culture, and Staphylococcus pseudintermedius was identified. The definitive diagnosis was caninesuperficial pyoderma. Apitoxin aquapuncture was performed to treat superficial pyoderma once a week for 5 weeks.The acupoints used in this clinical case included GV-14 (Da Zhui), BL-13 (Fei Shu), LI-11 (Qu Chi), BL-40 (WeiZhong), ST-36 (Zu San Li), SP-6 (San Yin Jiao), and SP-10 (Xue Hai). In addition, the herbal medicines Shi Wei Bai DuTang, Huang …

Variation In Genes Related To Cochlear Biology Is Strongly Associated With Adult-Onset Deafness In Border Collies, Jennifer S. Yokoyama, Ernst T. Lam, Alison L. Ruhe, Carolyn A. Erdman, Kathryn R. Robertson, Aubrey A. Webb, D. Colette Williams, Melanie L. Chang, Marjo K. Hytönen, Hannes Lohi, Steven P. Hamilton, Mark W. Neff

Anthropology Faculty Publications and Presentations

The domestic dog offers a unique opportunity to study complex disorders similar to those seen in humans, but within the context of the much simpler genetic backgrounds of pure breeds, which represent closed populations. We performed a whole-genome search for genetic risk factors of adult-onset deafness in the Border Collie, a breed of herding dog that relies on acute hearing to perceive and respond to commands while working. Adult-onset deafness in Border Collies typically begins in early adulthood and is similar to age-related hearing loss in humans. This earlier onset has particular impact on the utility of working Border Collies …

Mutations In Dmrt3 Affect Locomotion In Horses And Spinal Circuit Function In Mice, Lisa S. Andersson, Martin Larhammar, Fatima Memic, Hanna Wootz, Doreen Schwochow, Carl-Johan Rubin, Kalicharan Patra, Thorvaldur Arnason, Lisbeth Wellbring, Göran Hjälm, Freyja Imsland, Jessica Lynn Petersen, Molly E. Mccue, James R. Mickelson, Gus Cothran, Nadav Ahituv, Lars Roepstorff, Sofia Mikko, Anna Vallstedt, Gabriella Lindgren, Leif Andersson, Klas Kullander

Department of Animal Science: Faculty Publications

Locomotion in mammals relies on a central pattern-generating circuitry of spinal interneurons established during development that coordinates limb movement. These networks produce left–right alternation of limbs as well as coordinated activation of flexor and extensor muscles. Here we show that a premature stop codon in the DMRT3 gene has a major effect on the pattern of locomotion in horses. The mutation is permissive for the ability to perform alternate gaits and has a favorable effect on harness racing performance. Examination of wild-type and Dmrt3-null mice demonstrates that Dmrt3 is expressed in the dI6 subdivision of spinal cord neurons, takes …

Development Of Repressible Systems To Control Gene Expression In Vaccinia Virus, Allison Titong

Master's Theses

Two vaccinia virus (VACV) expression systems that contain elements from the lactose (lac) and the tetracycline (tet) operons of E. coli were developed to repress the expression of a reporter gene, enhanced green fluorescent protein (EGFP), in the presence of tet operon inducers. In the first system, lac and tet operon elements were arranged in a gene circuit, and in the presence of increasing concentrations of a lac operon inducer (isopropyl-β-D-thiogalactoside, IPTG), EGFP expression increased in a dose dependent manner and at high IPTG concentrations, expression reached the same levels as a positive control virus. Importantly, in the …

Let's Cure Cancer!, Wallace Morrison, Jessica Schneider, Sandra F. Amass

College of Veterinary Medicine eBooks

From ancient Egypt to modern times, scientists have been learning new ways to prevent, diagnose, and treat cancer. In this book, you’ll learn about cancer and how veterinarians, veterinary technicians, physicians, and other scientists are working together to find a cure for cancer in people and their pets.

Fat Dogs And Coughing Horses: Be The Vet! Solve The Case!, Kauline Cipriani Davis, Jessica Schneider, Henry Green Iii, Thad Blossom

College of Veterinary Medicine eBooks

This activity book highlights various careers in the veterinary profession and provides veterinary medical cases for the reader to solve.

This project was supported by the National Center for Research Resources and the Division of Program Coordination, Planning, and Strategic Initiatives of the National Institutes of Health through Grant Number R25 OD011115-04.

Also attached are the 6 fitness handouts - Canine Obesity, Dog Weight by Breed, Doggie Fitness, Eating Healthy, Healthy Treats, and Weather Tips.

How I Became A Veterinarian, Kauline Cipriani Davis, Sandra F. Amass, Paula Green

College of Veterinary Medicine eBooks

Veterinarians have fun and exciting jobs! In this book you will meet veterinarians from all over the world. These veterinarians work hard to make sure people and their animals stay healthy. Maybe one day you will be a veterinarian too!