A Novel Approach For Determination Of Chicken Sexing At An Early Stage Of Development By Using Loop-Mediated Isothermal Amplification Method, Sung Woo Kim, Jin Seok Choi, Neelesh Sharma, Yeoung Gyu Ko, Yoon Jung Do, Mijeong Byun, Hwan Hoo Seong, Soo Bong Park, Dong Kee Jeong
Turkish Journal of Veterinary & Animal Sciences
The monomorphism of newly hatched chicks is a major hurdle for poultry farmers at the time of sexing. The traditional sexing techniques are inaccurate and time-consuming. Therefore, the present study aimed to develop a loop-mediated isothermal amplification (LAMP)-based sex determination method to obtain a higher separation accuracy rate as compared to conventional methods. LAMP is a simple, quick, and relatively inexpensive diagnostic tool. Genomic DNA was extracted from the blood samples of Leghorn and Korean native chicks for LAMP. Two sets of primer were designed, consisting of four isothermal amplification primers in each, to amplify the female sex-specific sequence (HUR0417) …
The Accuracy Of The Patellar Reflex For Localization Of The Site Of A Single Level Thoracolumbar Disc Herniation In Dogs,
2015
TÜBİTAK
The Accuracy Of The Patellar Reflex For Localization Of The Site Of A Single Level Thoracolumbar Disc Herniation In Dogs, Mohammed Abdelhakiem, Yuuhi Asai, Hiroaki Kamishina, Masaaki Katayama, Yuji Uzuka
Turkish Journal of Veterinary & Animal Sciences
The patellar reflex was used in this study to determine the site of thoracolumbar spinal cord dysfunction (T3-L3 or L4-L6) as a result of disc herniation. The results of neurological examinations were compared with the findings of CT myelography. This study was conducted on 21 dogs, selected from a total of 26 dogs confirmed to have a single thoracolumbar lesion. Results revealed that the accuracy of the patellar reflex for lesion localization was 71.4%. The accuracy of the patellar reflex (normal or hyperreflexic) for lesion localization in the T3-L3 segment in 15 dogs was 100%, while the accuracy of the …
Frequency Of Hoof Conformation Faults And Disorders In Horses Of Several Breeds,
2015
TÜBİTAK
Frequency Of Hoof Conformation Faults And Disorders In Horses Of Several Breeds, Jaroslaw Luszczynski, Magdalena Pieszka, Anna Durmala, Weronika Pisarczyk, Romana Augustyn, Boguslawa Dlugosz
Turkish Journal of Veterinary & Animal Sciences
The aim of the study was to compare the frequencies of hoof conformation faults and disorders in the front and hind legs of horses of different breeds. The study was conducted on 346 horses over 3 years old (167 Anglo-Arabians, 130 Hucul horses, and 49 Silesian horses). In the study population, over 60% of the horses had some kind of hoof conformation faults and disorders. A significant difference in the incidence of hoof conformation faults (65.5%) and hoof disorders (35.5%) was shown in the analyzed population of horses. In the case of Hucul and Silesian horses, hoof conformation faults were …
Concentration Of Selected Radionuclides In Skulls Of European Badger Meles Meles From Eastern Poland,
2015
TÜBİTAK
Concentration Of Selected Radionuclides In Skulls Of European Badger Meles Meles From Eastern Poland, Andrzej Komosa, Ignacy Kitowski, Jolanta Orzel, Grzegorz Pitucha, Jan Solecki, Marcin Chrapowicki, Pawel Rozanski, Marian Flis
Turkish Journal of Veterinary & Animal Sciences
Concentration activity levels of selected radionuclides in skulls of European badger from eastern Poland were determined. It was found that studied skull bones contained on average 1.6 ± 1.7 Bq kg-1 of 137Cs (range: 0-6.4 Bq kg-1), 2.2 ± 0.87 Bq kg-1 of 90Sr (range: 0.93-3.9 Bq kg-1), and 8.4 ± 4.1 Bq kg-1 of 239+240Pu (range: 0-53 Bq kg-1). These values prove to be rather low in comparison with earlier literature data on anthropogenic radionuclides (being a result of the Chernobyl fallout) present in skulls of other mammals. This suggests that migration processes of anthropogenic radionuclides have caused a …
Analysis Of Acid?Base Disorders In Calves With Lactic Acidosis Using Aclassic Model And Strong Ion Approach,
2015
TÜBİTAK
Analysis Of Acid?Base Disorders In Calves With Lactic Acidosis Using Aclassic Model And Strong Ion Approach, Michal Bednarski, Robert Kupczynski
Turkish Journal of Veterinary & Animal Sciences
The objective of this study was to analyze the disorders of acid-base balance in calves with lactic acidosis using the classic model and the strong ion approach. The study included 40 calves: group I was the control group (n = 20) and group II consisted of calves with diarrhea and lactic acidosis (n = 20). The highest lactate concentration (5.49 ± 2.58 mmol/L) was documented in diarrheic calves. The diarrheic calves presented with significantly lower pH, pCO2, and concentrations of HCO3- (P < 0.01), and with higher anion gap (AG), strong ion difference (SID3, SID7), and strong ion gap (SIG) (P < 0.01) than the controls. In the group of diarrheic calves lactic acid correlated with AG (r = 0.684, P < 0.01), SID3 (r = 0.718, P < 0.01), SID7 (r = 0.494, P = 0.03), and SIG (r = 0.561, P = 0.01). There was a negative correlation between lactate and effective SID (SIDEff) (r = -0.499, P = 0.02), and total plasma concentration of nonvolatile buffers (ATot ) (r = -0.361, P = 0.04). The results indicated that in lactic acidosis there were specific disturbances with an increased concentration of unmeasured strong ions.
Changes In Arginase Activity And Ast Enzyme Levels In The Cardiac Andskeletal Muscle And Liver Of Lambs With White Muscle Disease, Başak Hanedan, Mi̇ne Eri̇şi̇r, Fati̇h Mehmet Kandemi̇r, Mustafa Özkaraca
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to evaluate arginase activity and aspartate aminotransferase (AST) levels in the tissues of the cardiac and skeletal muscle and liver of lambs with white muscle disease (WMD). The cardiac and skeletal muscle and liver tissues were obtained from 8 lambs with WMD and 9 apparently healthy lambs. The diagnosis of WMD was made with the detection of hyaline degeneration upon histopathological examination. A significant increase in arginase activity in the cardiac and skeletal muscle and liver tissues and a significant decrease in AST levels in the cardiac muscle were detected in the WMD group …
Heparin-Induced In Vitro Capacitation Changes Of Swamp Buffalo Spermatozoa,
2015
TÜBİTAK
Heparin-Induced In Vitro Capacitation Changes Of Swamp Buffalo Spermatozoa, Dibyajyoti Talukdar, Kutubuddin Ahmed, Sourabh Deori, Gopal Chandra Das
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to evaluate heparin-induced in vitro capacitation-associated changes in spermatozoa of swamp buffalo. Therefore, freshly ejaculated and washed spermatozoa of 8 swamp buffalo bulls were capacitated in vitro in TALP medium supplemented with BSA, heparin, and HEPES buffer at a concentration of 6 × 109 spermatozoa/mL at 37 °C for 6 h. Capacitation status of spermatozoa in terms of the hyperactivated motility, acrosome membrane integrity, total hypoosmotic swelling test (HOST), activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), and sperm membrane protein (SMP) and cholesterol content were estimated for each ejaculate at 1-h intervals …
Effects Of 16l : 8d Photoperiod On Growth Performance, Carcass Characteristics,Meat Composition, And Blood Parameters Of Pekin Ducks, Evren Erdem, Esi̇n Ebru Onbaşilar, Özlem Gücüyener Hacan
Turkish Journal of Veterinary & Animal Sciences
No abstract provided.
Breed, Parity, And Cycle Season Effects On Life-Timereproduction In Bitches: A Retrospective Study,
2015
TÜBİTAK
Breed, Parity, And Cycle Season Effects On Life-Timereproduction In Bitches: A Retrospective Study, Bülent Polat, Armağan Çolak, Mehmet Cengi̇z, Orçun Cannazi̇k, Armağan Hayirli
Turkish Journal of Veterinary & Animal Sciences
The aim of this retrospective study was to evaluate the effects of breed, parity, and season on the reproductive parameters in bitches reared under a temperate climate. Breeding and pregnancy statuses, proestrus, estrus, and pregnancy durations, litter size, and cycle interval were recorded from German shepherds (GS, n = 34), Labrador retrievers (LA, n = 23), Belgian Malinois dogs (BM, n = 13), and Pointers (PO, n = 9) up to 10th parities. The mean age at first breeding (464.8 ± 26.2 days, mean ± SD) and the pregnancy rate (74.6%) were not different among breeds. The pregnancy duration was …
The Effects Of Dry-Off Therapy On Milk Somatic Cell Count In Saanen Goats,
2015
TÜBİTAK
The Effects Of Dry-Off Therapy On Milk Somatic Cell Count In Saanen Goats, Ayhan Baştan, Seçki̇n Salar, Duygu Baki̇ Acar, Mürşi̇de Ayşe Demi̇rel, Mehmet Cengi̇z, İsfendi̇yar Darbaz, Gaye Bulut
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the effectiveness of dry-off antibiotic therapy and teat sealant on somatic cell count (SCC) in Saanen goats. The goats were randomly divided into 3 groups. In Groups I (n = 50) and II (n = 50), the goats were treated with intramammary antibiotics and a combination of intramammary antibiotics and internal teat sealant at dry-off, respectively. The animals in Group III (n = 50) were designated as the control group. For the SCC analysis and bacteriological examination, milk samples (n = 900) were collected separately from each udder half during routine morning …
Determination Of Serum Cardiac Biomarkers And Plasma D-Dimer Levelsin Anemic Sheep With Babesiosis,
2015
TÜBİTAK
Determination Of Serum Cardiac Biomarkers And Plasma D-Dimer Levelsin Anemic Sheep With Babesiosis, Özlem Orunç Kilinç, Yaşar Göz, Nazmi̇ Yüksek, Yildiray Başbuğan, Ali̇ Bi̇lgi̇n Yilmaz, Ahmet Duran Ataş
Turkish Journal of Veterinary & Animal Sciences
In this study, the cardiac effects of anemia and venous thromboembolism (VTE), reportedly caused by the hemolyzed erythrocytes occurring in sheep with babesiosis, were investigated using cardiac markers and D-dimer (DD). The study included 34 sheep: 24 infected Akkaraman sheep (1-3 years old, diagnosed with babesiosis based on clinical and laboratory findings) and a control group of 10 noninfected healthy sheep of the same breed and age. Hematocrit (Hct) levels were measured in blood obtained from both groups. Cardiac troponin I (cTnI), creatine kinase-MB (CK-MB), and aspartate aminotransferase (AST) levels were measured in serum samples. In addition, the levels of …
The Effects Of Different Salting And Preservation Techniques Ofkaşar Cheese On Cheese Quality,
2015
TÜBİTAK
The Effects Of Different Salting And Preservation Techniques Ofkaşar Cheese On Cheese Quality, Asya Çeti̇nkaya, Mustafa Atasever
Turkish Journal of Veterinary & Animal Sciences
The aim of this research is to determine the effects of different salting and preservation techniques on the quality of kaşar cheese. Physical, chemical, and microbiological analyses were performed on days 1, 7, 15, 30, 60, 90, and 120 of the maturation of 270 samples of kaşar cheese produced for this research. Additionally, sensory analyses were performed on days 30, 60, 90, and 120 of maturation. The total number of aerobic mesophilic bacteria, Lactococcus, Staphylococcus-Micrococcus, and psychotropic bacteria was lower and the number of yeast-mold and lactobacilli was higher in cheese examples salted in a boiler than those salted in …
Clinical And Radiographic Characterization Of Xenotransplantation Of Rat Bonemarrow-Derived Mesenchymal Stem Cells For Repair Of Radial Defects Of Rabbit, Betania Monteiro, Lukiya Favarato, Pablo Carvalho, Barbara Okano, Maria Antonia Menegatti, Alvaro Oliveira, Bianka Santos, Ricardo Del Carlo
Turkish Journal of Veterinary & Animal Sciences
This study aimed to evaluate the clinical and radiographic aspects of xenogenic transplantation of rat bone marrow-derived mesenchymal stem cells (MSCs) for the repair of radial bone defects of rabbit. Bone marrow derived MSCs collected from Wistar rats were expanded, differentiated, and labeled in the laboratory. In the fourth passage, the cultures were pelleted in aliquots of 107 cells/50 μL and transplanted in situ into the lesions. Thirty-six rabbits were used in the in vivo study. In all animals, defects were created by removing a bone segment of 1.0 cm in length in the right radius. The rabbits were divided …
Fattening Performance And Carcass Characteristics Of Turkish Indigenoushair And Honamlı Goat Male Kids,
2015
TÜBİTAK
Fattening Performance And Carcass Characteristics Of Turkish Indigenoushair And Honamlı Goat Male Kids, Ahmet Hamdi̇ Aktaş, Beki̇r Gök, Serkan Ateş, Mehmet Emi̇n Teki̇n, İbrahi̇m Halici, Hüseyi̇n Baş, Hakan Erduran, Shinan Kassam
Turkish Journal of Veterinary & Animal Sciences
This study was undertaken to measure the effect of fattening duration (60, 80, and 100 days) on growth performance and carcass characteristics of male kids from indigenous Hair and Honamlı goat breeds reared under intensive fattening systems in Turkey. Fattening duration had a significant effect (P < 0.001) on the fattening performance and a number of carcass characteristics of kids. An interaction was detected between genotype and fattening duration for average daily gain (ADG). Hair goat kids grew at a faster rate than Honamlı kids in the 20-40 day and 40-60 day fattening periods, but they maintained relatively similar ADGs within other fattening periods. On average, over the course of the fattening trial, Honamlı kids grew at an average daily rate of 209 g/head, while Hair goat kids grew at a daily rate of 231 g/head. Dressing percentage and carcass lean and fat percentages of kids increased (P < 0.05-0.001) with slaughter age and weight for both genotypes, whereas the percentage of bone decreased (P < 0.001) with each successive fattening period. No genotypic differences were detected for a number of carcass characteristics at the time of slaughter, with the exception of relatively higher (P < 0.01) fat deposit rates within the kidney and pelvic areas for Hair kids.
Prevalence Of Blaz Gene And Other Virulence Genes In Penicillin-Resistant Staphylococcus Aureus Isolated From Bovine Mastitis Cases In Gansu, China, Feng Yang, Qi Wang, Xurong Wang, Ling Wang, Min Xiao, Xinpu Li, Jinyin Luo, Shidong Zhang, Hongsheng Li
Turkish Journal of Veterinary & Animal Sciences
Staphylococcus aureus is a major etiological agent of bovine mastitis worldwide. In this study, 37 strains of S. Aureus resistant to penicillin were isolated from bovine mastitis cases in Gansu Province for investigating blaZ and virulence-related genes, including tst, eta, etb, lukPV, lukED, lukM, hla, hlb, hld, and edin. Antibiotic resistance was based on disk diffusion method and blaZ and virulence-associated genes were studied by polymerase chain reaction. Penicillin resistance gene blaZ was detected in 35/37 (94.6%) of penicillin-resistant S. Aureus isolates. tst, lukPV, lukED, hla, hlb, and hld were observed in 5.4%, 2.7%, 89.2%, 70.3%, 73.0%, and 70.3% of …
Effects Of Long-Term Starvation And Refeeding On Fatty Acid Metabolism-Relatedgene Expressions In The Liver Of Zebrafish, Danio Rerio, Ayşe Ölmez, Mehtap Bayir, Chunfang Wang, Abdulkadi̇r Bayir
Turkish Journal of Veterinary & Animal Sciences
A 70-day starvation was conducted to determine the effects of long-term starvation on whole-body polyunsaturated fatty acid (PUFA) composition and fatty acid metabolism-related gene expression in the liver of zebrafish, Danio rerio. The starvation period was followed by a 15-day refeeding period to test recovery of both PUFA composition and gene expression levels. It was found that n-3 PUFA and arachidonic acid levels increased during starvation and returned to normal values after refeeding (P < 0.05). Expression of seven (elovl5, fads2, cpt1-β, acox1, acadvl, fabp1a, and fabp7a) of eight total genes downregulated significantly towards the end of the starvation. As a result of refeeding, gene expression of elovl5 and ppar-α returned to the normal levels. Therefore, it was concluded that, in general, mRNA expression of genes involved in fatty acid metabolism was negatively influenced by long-term starvation (70 days); there was no direct relationship between whole-body fatty acid composition and hepatic gene expressions; and 15-day refeeding was insufficient for recovery of starvation-based metabolic losses in zebrafish.
Serum Pharmacokinetics And Tissue Concentrations Of A Newrecrystallized Enrofloxacin Hydrochloride-Dihydrate In Hamsters,
2015
TÜBİTAK
Serum Pharmacokinetics And Tissue Concentrations Of A Newrecrystallized Enrofloxacin Hydrochloride-Dihydrate In Hamsters, Alma Carrascosa, Alejandro De La Peña, Lilia Gutierrez, Hector Sumano
Turkish Journal of Veterinary & Animal Sciences
Pharmacokinetics (PK), pharmacodynamics (PD), and PK/PD ratios of a new solvate of enrofloxacin (enrofloxacin hydrochloride-dihydrate; enro-C), were studied in hamsters. Enrofloxacin from Baytril1397904493 5% (enroR) served as the reference preparation. Two groups of 60 Syrian golden hamsters were intramuscularly injected individually with 10 mg/kg of enroR or with enro-C. Tissue and serum samples were obtained for 72 h; enrofloxacin concentrations were determined by HPLC with UV detection. Ninety percent minimum inhibitory concentrations (MIC90) were determined for strains of methicillin-resistant Staphylococcus aureus, Leptospira interrogans, and Escherichia coli. All PK variables were statistically different between groups (P < 0.01). CMAX of enrofloxacin for enro-C was 17.3 μg/mL and it was 2.6 μg/mL for enroR. AUC was considerably higher for enro-C (459.2 μg/mL h vs. 19.9 μg/mL h). There were no statistically significant differences in MIC90 values between enroR and enro-C. Tissue concentrations of enro-C in all cases were higher and remained above the MIC for longer periods than those of enroR. Relevant PK/PD ratios for enrofloxacin (AUC/MIC ? 125 and CMAX > MIC = 10-12) are consequently …
In Situ Degradation Of Almond (Prunus Dulcis L.) Hulls, A Potential Feed Material For Ruminants,
2015
TÜBİTAK
In Situ Degradation Of Almond (Prunus Dulcis L.) Hulls, A Potential Feed Material For Ruminants, Saeid Jafari, Alireza Alizadeh, Ali Imni, Goh Yong Meng, Mohamed Ali Rajion, Mahdi Ebrahimi
Turkish Journal of Veterinary & Animal Sciences
The nutritive values of almond hulls (Ahs), alfalfa (ALF), and sugar beet pulp (SBP) were determined using the in situ nylon bag technique. The ruminal dry matter (DM) degradation kinetics of 4 varieties of Ahs were compared with ALF and SBP. The almond varieties tested were Rabbi (RAB), Mamaii (MAM), Shahrud 15 (SH15), and Shokufe (SH). Samples were incubated in triplicate at 0, 4, 8, 12, 16, 24, 48, 72, and 96 h in 3 rumen-fistulated steers. The average total degradability fraction (TDF) of DM in Ahs was 89.00%. Although the TDF of Ahs (86.50%) was lower than that of …
The Prediction Of The Prevalence And Risk Factors For Subclinical Heifermastitis In Turkish Dairy Farms,
2015
TÜBİTAK
The Prediction Of The Prevalence And Risk Factors For Subclinical Heifermastitis In Turkish Dairy Farms, Ayhan Baştan, Seçki̇n Salar, Mehmet Cengi̇z, İsfendi̇yar Darbaz, Mürşi̇de Ayşe Demi̇rel, Doğukan Özen
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the risk factors for subclinical heifer mastitis and to create a model that can predict the prevalence of subclinical mastitis of pregnant heifers in farms in Turkey. Lacteal secretion samples were taken from 439 pregnant (6-9 months) heifers and bacteriological analyses were performed. In this study, 37.47% of the samples were determined to be infected. In lacteal secretion samples, the isolation rate of coagulase-negative staphylococci and Staphylococcus aureus was 44.83% and 35.71%, respectively. The incidence of mastitis was calculated as 42.87 ± 4.12%. An eight-question survey was conducted. Using the data collected, …
Macroanatomical Characteristics Of Plexus Brachialis And Its Branches In Martens (Martes Foina),
2015
TÜBİTAK
Macroanatomical Characteristics Of Plexus Brachialis And Its Branches In Martens (Martes Foina), Yasi̇n Demi̇raslan, Mevlüt Aykut, Özcan Özgel
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was to determine the macroanatomical characteristics of nerves forming the plexus brachialis and originating from plexus in beech martens. A total of 6 beech martens (3 males and 3 females) that died in traffic accidents and were brought to Mehmet Akif Ersoy University's Faculty of Veterinary Medicine, Department of Anatomy on various dates were used in this study. Nerves forming the plexus brachialis and originating from this plexus in martens were dissected and their results were recorded. According to the obtained results, it was observed that the plexus brachialis in beech martens was composed of …
