The Assembly Of Virus-Like Particles Of Porcine Transmissible Gastroenteritisvirus In Vitro By Baculovirus Expression System,
2015
TÜBİTAK
The Assembly Of Virus-Like Particles Of Porcine Transmissible Gastroenteritisvirus In Vitro By Baculovirus Expression System, Song Zhenhui, Haibo Feng, Zhi Zhu, Xianjin Dai, Yue Zhou, Yuntian Li, Xinzhi Cao
Turkish Journal of Veterinary & Animal Sciences
Virus-like particles (VLPs) produced by recombinant expression of the major viral structural proteins could be an attractive method for transmissible gastroenteritis virus (TGEV) assembly and release. In this study, the M gene, sM gene, and N gene of TGEV were inserted into donor plasmid pFastBac Dual. Those recombinant donor plasmids were transformed into E.coli DH10Bac respectively. Recombinant baculoviruses were obtained by transfection of the Sf9 cells with Bacmid-M, Bacmid-sM, and Bacmid-N. The results of indirect immunofluorescence assay showed that recombinant M protein, sM protein, and N protein were expressed successfully. In vitro experiments of VLP assembly of TGEV were performed …
Comparison Of In Vitro And In Vivo Antibacterial Efficacy For The Control Of Flavobacterium Psychrophilum In Rainbow Trout (Oncorhynchus Mykiss)Fry: The First Genotypical Evidence In West Aegean Region Of Turkey, Murat Boyacioğlu, Cavi̇t Kum, Şükrü Kirkan, Seli̇m Sekki̇n, Uğur Parin, Ümi̇t Karademi̇r, Ferda Akar
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to investigate the presence of Flavobacterium psychrophilum and to examine the in vitro and in vivo efficacy of enrofloxacin (ENR), doxycycline (DOX), and florfenicol (FFC) treatment in rainbow trout fry (Oncorhynchus mykiss Walbaum) against F. Psychrophilum. A total of 220 naturally infected rainbow trout fry were examined and 26 bacterial isolates were phenotypically and genotypically identified. Minimum inhibitory concentrations (MICs) of ENR, DOX, and FFC were determined. ENR was the most active, with an MIC for 90% of the isolates (MIC90) of 4 µg/mL. DOX was less active, with an MIC90 of 32 µg/mL …
Comparing Levels Of Certain Heavy Metals And Minerals And Antioxidativemetabolism In Cows Raised Near And Away From Highways*,
2015
TÜBİTAK
Comparing Levels Of Certain Heavy Metals And Minerals And Antioxidativemetabolism In Cows Raised Near And Away From Highways*, Hüsametti̇n Eki̇ci̇, Özkan Şi̇mşek, Şevket Arikan, Meryem Eren, Bayram Güner
Turkish Journal of Veterinary & Animal Sciences
The scope of this study was to investigate the concentrations of certain heavy metals, mineral levels, and antioxidative metabolism in cows raised near (300 m) and away from highways (2.5 km) in Çankırı Province. For this purpose, blood samples were collected from 100 cows (aged 3-5 years) raised near and away from highways in this province. Concentrations of certain heavy metals and minerals in the serum were analyzed on an ICP-MS device. Additionally, lipid peroxidation product malondialdehyde (MDA) in the plasma and antioxidative defense system indicators, erythrocyte superoxide dismutase (SOD) and catalase (CAT) activities, were measured with a spectrophotometer. As …
Prevalence Of Toxoplasma Gondii In Sheep Meats Purchased From Retailstores In Central Anatolia, Turkey,
2015
TÜBİTAK
Prevalence Of Toxoplasma Gondii In Sheep Meats Purchased From Retailstores In Central Anatolia, Turkey, Kader Yildiz, Fatma Çi̇ğdem Pi̇şki̇n, Armağan Erdem Ütük, Sami̇ Gökpinar
Turkish Journal of Veterinary & Animal Sciences
Toxoplasmosis is one of the most important foodborne parasitic diseases of humans. In particular, sheep muscles are significant sources of infection in the transmission of toxoplasmosis. Carnivorism is the most important transmission route for human populations. The aim of this study was to detect the prevalence of Toxoplasma gondii tissue cysts in sheep meats in retail stories of Turkey. A total of 250 boneless sheep meat samples were purchased from randomly selected retail stores in different locations of Ankara and Kırıkkale provinces of Turkey. The homogenized meat samples were centrifuged with Percoll dilutions. The tissue cysts were removed by pipette …
Y Chromosome Haplotype Characterization Of Tunisian Sheep Breeds,
2015
TÜBİTAK
Y Chromosome Haplotype Characterization Of Tunisian Sheep Breeds, Samia Kdidi, Mohamed Habib Yahyaoui, Belen García-Manrique, Pilar Sarto, Mohsen Ben Sassi, Touhami Khorchani, Jorge Hugo Calvo
Turkish Journal of Veterinary & Animal Sciences
This study aimed to investigate Y chromosomal haplotypes in the main Tunisian sheep breeds. For this purpose, we sequenced 2 regions of SRY (549-bp and 598-bp fragments) and 1 region in each of the AMELY and DBY genes in Barbarin, Western Thin Tail, Sicilo Sarde, and Black Thibar breeds. In addition, we analyzed the diversity at the SRYM18 microsatellite locus and detected 4 alleles of 139, 141, 143, and 145 bp. The sequencing analysis did not reveal any polymorphism in the AMELY or DBY regions; however, 2 samples carried the G allele in the 549-bp fragment of SRY. A total …
Effects Of Fermented Cottonseed Meal On The Growth Performance, Apparent Digestibility, Carcass Traits, And Meat Composition In Yellow-Feathered Broilers, Cunxi Nie, Wenju Zhang, Wenxia Ge, Yongqiang Wang, Yangfeng Liu, Jiancheng Liu
Turkish Journal of Veterinary & Animal Sciences
The effects of supplementing broilers' diets with fermented cottonseed meal (FCSM) on the broilers' performance, apparent digestibility, carcass traits, and meat composition were studied. A total of 180 yellow-feathered chickens of 21 days old were randomly divided into three groups (six replicates of 10 broilers each): 1) a control group (Con), 2) a treatment group of FCSM by Candida tropicalis (Ct), and 3) a treatment group of FCSM by C. Tropicalis plus Saccharomyces cerevisiae (Ct-Sc). Results showed that FCSM supplementation improved the average daily gain and gain-to-feed ratio from the 43rd to 64th and the 21st to 64th day, respectively …
Concentrations Of Manganese, Iron, And Strontium In Bones Of Thedomestic Dog (Canis Lupus Familiaris),
2015
TÜBİTAK
Concentrations Of Manganese, Iron, And Strontium In Bones Of Thedomestic Dog (Canis Lupus Familiaris), Halina Budis, Elzbieta Kalisinska, Natalia Lanocha, Danuta Kosik_Bogacka
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine concentrations of manganese (Mn), iron (Fe), and strontium (Sr) in the cartilage, compact bone, spongy bone, and cartilage with adjacent compact bone of the domestic dog (Canis lupus familiaris). The experiment was carried out on bones from the hip joints of 24 dogs (dogs of 8 years) from northwestern Poland. The concentrations of Mn, Fe, and Sr were assessed by atomic absorption spectrophotometry. Fe concentration was the highest in the cartilage and compact bone. Mn concentration was the lowest in all three types of bone material and had a significantly lower concentration …
Effects Of High-Level Chromium Methionine Supplementation In Lambs Fed A Corn-Based Diet On The Carcass Characteristics And Chemical Composition Of The Longissimus Muscle, Berenice Sanchez-Mendoza, Antonio Aguilar-Hernandez, Maria A. Lopez-Soto, Alberto Barreras, Alfredo Estrada-Angulo, Francisco Javier Monge Navarro, Noemi G. Torrentera, Richard A. Zinn, Alejandro Plascencia
Turkish Journal of Veterinary & Animal Sciences
Twenty-four male lambs (24.93 ± 0.93 kg) were used in order to evaluate the effects of chromium methionine (Cr) supplementation in high-energy finishing diets on the carcass characteristics and chemical composition of the longissimus muscle (LM). Treatments were 0.00, 0.60, 1.20, and 1.80 mg Cr/lamb daily. The experiment lasted 56 days. There were no effects of treatments on dry matter intake, feed to gain ratio, and final weight, which averaged 35.24 ± 1.01 kg. Supplemental Cr linearly decreased fat thickness enough to improve the estimated yield grade from 1.82 to 1.42 with no effect on the other carcass traits measured. …
The Effect Of Macromolecule And Growth Factor Combinations On In Vitrodevelopment Of Bovine Embryos,
2015
TÜBİTAK
The Effect Of Macromolecule And Growth Factor Combinations On In Vitrodevelopment Of Bovine Embryos, Alper Koçyi̇ği̇t, Mesut Çevi̇k, Uğur Şen, Mehmet Kuran
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to determine the effects of different macromolecule sources added to synthetic oviduct fluid (SOF) culture medium supplemented with growth factors on the development of bovine embryos and blastocyst morphology. Zygotes were distributed into 5 treatment groups. Cleavage, morula, and blastocyst rates were evaluated under a stereomicroscope. Trophectoderm (TE) and inner cell mass (ICM) cells were determined by differential staining method. It was found that bovine serum albumin (BSA), either alone or in combination with growth factors, as compared to the control or polyvinyl-alcohol (PVA) resulted in higher embryo yield and faster development during early bovine embryo …
Comparative Effects Of Sirolimus-Eluting Versus Bare Metal Stents On Neointimal Hyperplasia In Balloon-Injured External Iliac Artery Of Rabbits, Atul Patel, Pinesh Parikh, Deepak Patil, Anila Mathew, Suhas Lele, Mohan Rajapurkar, Pradip Patil, Akhilesh Kumar, Priti Vani
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to check effectiveness of drug-eluting stents for prevention of restenosis resulting from neointimal hyperplasia. Twenty-one sirolimus-eluting stents (SESs) and bare metal stents (BMSs; control) were compared by implantation into left and right balloon-injured external iliac arteries of New Zealand White rabbits, respectively. Under general anesthesia and angiography, using 3-mm coronary balloon catheters, stents were successfully deployed. Anticoagulant therapy was not given to any animal. Rabbits were allotted into three groups at 7, 14, and 21 days (7 rabbits in each group). Each stented vessel of sacrificed rabbits was subjected to a resin-embedding technique and …
Impact Of B. Melitensis Rev-1 Vaccination On Brucellosis Prevalence,
2015
TÜBİTAK
Impact Of B. Melitensis Rev-1 Vaccination On Brucellosis Prevalence, Adosinda Maria Coelho, Maria De Lurdes Pinto, Juan Garcia Diez, Ana Claudia Coelho
Turkish Journal of Veterinary & Animal Sciences
Trás-os-Montes e Alto Douro, in the northeastern region of Portugal, previously exhibited the highest brucellosis prevalence in the entire small ruminant population of Portugal. Consequently, a vaccination program of the whole population with Brucella melitensis Rev-1 was carried out from 2001 to 2004, and further compulsory Rev-1 vaccination of 3- to 6-month-old lambs and kids was carried out between 2005 and 2007. The prevalence of brucellosis decreased by 71.42% in 2004, with a 2-fold decrease occurring from 2005 to 2007. The reduction of brucellosis prevalence was statistically significant (P < 0.001) according to flock size. By species, brucellosis prevalence decreased 14.5-fold in sheep and 5.5-fold in goats in 2004. Regarding flock constitution, it decreased by 77% for pure flocks and 73% in mixed flocks in 2004. Regarding the animals involved in production, brucellosis prevalence decreased during the 7-year period. It was concluded that Rev-1 vaccination of the whole population was effective in decreasing brucellosis prevalence. These results contribute to the scarce information available regarding the effect of Rev-1 vaccination on different characteristics of flocks. They may be used to improve the efficiency of brucellosis eradication programs within livestock management.
Relationships Of Fatty Acid Group Contents In Milk And Reproductiveperformance In Holstein Cows,
2015
TÜBİTAK
Relationships Of Fatty Acid Group Contents In Milk And Reproductiveperformance In Holstein Cows, Jaromír Duchácek, Jan Beran, Martin Ptácek, Ludek Stádník, Monika Okrouhla, Renata Tousová, Martina Dolezalova
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to evaluate the relationships between milk fatty acid group contents and subsequent reproductive performance in Holstein cows. A total of 27 cows in the 1st to 4th lactations were included in the evaluation. Cows were divided into primiparous and multiparous groups. Daily milk yields increased in the first 8 weeks of lactation. Body condition score decreased until the third month of lactation. As the cows gradually recovered from the negative energy balance, the contents of saturated fatty acids (SFAs) increased, whereas the contents of monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs) decreased. …
Estimating Allele Frequencies Of Some Hereditary Diseases In Holstein Cattlereared In Burdur Province, Turkey,
2015
TÜBİTAK
Estimating Allele Frequencies Of Some Hereditary Diseases In Holstein Cattlereared In Burdur Province, Turkey, Özgecan Korkmaz Ağaoğlu, Ali̇ Reha Ağaoğlu, Mustafa Saatci
Turkish Journal of Veterinary & Animal Sciences
The economics of dairy cattle breeding around the world has been significantly affected by the autosomal recessive hereditary diseases of bovine leukocyte adhesion deficiency (BLAD), factor XI deficiency (FXID), bovine citrullinemia (BC), and deficiency of uridine monophosphate synthase (DUMPS). The aim of this study was to determine BLAD, FXID, BC, and DUMPS alleles in Holstein cattle raised in Burdur Province in Turkey. This study involved taking blood samples randomly from 500 head of Holstein cattle at various dairy farms in different towns in Burdur Province. Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis was employed to identify cows …
The Effect Of Different Body Weight Categories On Plasma Macromineral Levels In Four Close-Bred Flocks Of Adult Japanese Quails (Coturnix Coturnix Japonica), Ahmed Sultan Jatoi, Abdul Waheed Sahota, Muhammad Akram, Khalid Javed, Muhammad Hayat Jaspal, Jibran Hussain, Shahid Mehmood, Yassar Abbas, Amjad Hussain Mirani, Hafiz Muhammad Ishaq
Turkish Journal of Veterinary & Animal Sciences
The present study was conducted to investigate plasma macrominerals (Ca, P, Na, K, and Mg) in adult male and female quails according to different body weights in four close-bred flocks of Japanese quails (Coturnix coturnix japonica) maintained at the Avian Research and Training Center of the University of Veterinary and Animal Sciences, Lahore. The results showed that mean plasma Na concentration was significantly (P < 0.05) different in female quails with respect to body size categories. The interaction between flocks and body size for plasma Ca levels was significant (P < 0.05) in both sexes of quails, whereas plasma Na was significant (P < 0.05) in female quails. The mean plasma P and K levels in imported and local flocks of Japanese quails were significantly (P < 0.05) different in female quails, whereas plasma Mg was significantly (P < 0.05) different in male quails. However, with respect to body size categories, plasma P, K, and Mg were significantly (P < 0.05) different in female quails only. The interaction between flocks and body size was significant for K and P in female quails and for plasma Mg levels in both sexes of quails.
Textural Acceptability Of Prepared Fish Sausages By Controlling Textural Indicators,
2015
TÜBİTAK
Textural Acceptability Of Prepared Fish Sausages By Controlling Textural Indicators, Mehmet Tolga Di̇nçer, Şükran Çakli
Turkish Journal of Veterinary & Animal Sciences
The purpose of this paper is to verify the textural quality and acceptability of two different prepared emulsion-type fish sausages that were evaluated by using rainbow trout (Oncorhynchus mykiss) and saithe (Pollachius virens) fillets. Texture properties of the fish sausages were compared with three different emulsion-type sausages (chicken, turkey, and beef sausages) using texture profile analysis (TPA), shear test, Kramer shear test, pate penetration test, cooking loss, and expressible moisture. According to the TPA and firmness values, some textural indicators were found significantly weak (P < 0.05), but they met consumers preferences.
Analysis Of Apolipoprotein A-Iv Expression And The Levels Of Blood Plasmamain Lipid Fractions Of Calves During The First Week Of Life Before And After Theadministration Of Milk Replacer Supplemented With Lactose, Katarzyna Michalek, Alicja Dratwa-Chalupnik, Magdalena Jaszczuk
Turkish Journal of Veterinary & Animal Sciences
The current study presents an analysis of main lipid fractions and expression of apolipoprotein A-IV (apoA-IV) of the blood plasma of calves during the first week of life, before and after administration of a milk replacer with additional quantity of lactose. The experiment was performed on 7 male Polish-Friesian calves, var. Black-and-White. Blood samples were collected before the morning feeding from the 6th to 8th day of life. In the evening on the 6th day of the experiment and in the morning on the 7th day of the experiment, monohydrate lactose in an amount of 2 g per 1 kg …
Effects Of Spray-Dried Porcine Plasma Replacement With Tryptophan, Pyridoxine, And Niacin Supplementation On Feed Intake And Growth Performance Of Weaning Piglets, Wantamas Jantasin, Liang Chou Hsia
Turkish Journal of Veterinary & Animal Sciences
The purpose of this experiment was to investigate the effects of feeding a low crude protein diet with supplemental dietary tryptophan in combination with pyridoxine and niacin compared with spray-dried porcine plasma (SDPP) supplementation on the performance and feed intake of weaning pigs. A total of 36 crossbred (Landrace × Yorkshire × Duroc) weaned female pigs of 28 days old were reared under three dietary treatments. Treatments were 1) corn-soybean meal diet, 2) 0.30% synthetic tryptophan + pyridoxine + niacin, and 3) 5% SDPP. The experimental results indicated no significant difference (P > 0.05) in feed intake for pigs fed the …
Influence Of Individual Or Mixed Cellulase And Xylanase Mixture On In Vitro Rumen Gas Production Kinetics Of Total Mixed Rations With Different Maize Silage And Concentrate Ratios, Mona Mohamed Mohamed Yasseen Elghandour, Ahmed Eid Kholif, Ofelia Márquez-Molina, José Fernando Vázquez-Armijo, Anil Kumar Puniya, Abdelfattah Zeidan Mohamed Salem
Turkish Journal of Veterinary & Animal Sciences
This study assessed the effects of cellulase, xylanase, and their mixture (1:1, v/v) on in vitro rumen fermentation of five mixed rations with different maize silage (F) to concentrate (C) ratios (0F:100C, 25F:75C, 50F:50C, 75F:25C, 100F:0C). Samples were incubated using rumen inoculum from Brown Swiss cows fed ad libitum a total mixed ration of concentrate and alfalfa hay (1:1). Gas production (GP) was recorded after 2, 4, 6, 8, 10, 12, 24, 36, 48, and 72 h of incubation. Interaction effects (P < 0.0001) were observed between type of ration and enzyme for discrete lag time prior to GP. Ration type affected asymptotic GP (linear and quadratic, P < 0.0001) and in vitro GP at 24 h onward. Moreover, rations affected pH (linear and quadratic, P < 0.0001), dry matter degradability (quadratic, P = 0.05), metabolizable energy (linear, P = 0.038), short-chain fatty acids (linear, P = 0.0005), gas yield from truly digested dry matter at 24 h (linear, P = 0.0026), and microbial crude protein yield (linear, P = 0.0264). The most effective rations for in vitro GP during different times differed between different measured parameters. Enzyme administration was more effective when the ratio of maize silage was increased.
Deactivation Of Tannins In Raisin Stalk By Polyethylene Glycol-6000 Effect Onfeed Intake, Digestibility, And Nitrogen Retention In Sheep, Manocher Souri, Laleh Angaji, Mohammad Mehdi Moeini
Turkish Journal of Veterinary & Animal Sciences
An experiment was conducted to assess the effects of polyethylene glycol (PEG-6000) and urea on feed intake, nitrogen retention, and nutrient digestibility in sheep fed raisin stalk. Sixteen male fat-tailed Sanjabi sheep (26 ± 0.66 kg) were randomly assigned to one of 4 dietary treatments: raisin stalk (control; RS), raisin stalk supplemented with 3% urea and 4% molasses (RSU3%); 5% urea and 7% molasses (RSU5%); or 5% urea, 7% molasses plus 5% PEG (RSU-PEG) per DM of raisin stalk. The urea and molasses were dissolved in distilled water and then mixed into a solid ingredient. Polyethylene glycol was also sprayed …
The Effects Of High Setter And Hatcher Temperatures During Incubation On Slaughter Weight And Carcass Yield In Broilers,
2015
TÜBİTAK
The Effects Of High Setter And Hatcher Temperatures During Incubation On Slaughter Weight And Carcass Yield In Broilers, Aydin İpek, Arda Sözcü
Turkish Journal of Veterinary & Animal Sciences
This study was carried out with the aim of determining the effects of higher setter and hatcher temperatures on slaughter weight and carcass yield. In experiment 1, setter temperatures were applied as control (37.8-38.2 °C) and high (38.9-40.0 °C) between days 10 and 18 during incubation. In experiment 2, hatcher temperatures were applied as control (36.8-37.0 °C) and high (38.8-39.0 °C) during the hatching period. A total of 240 chicks from each experiment were randomly selected after the hatching process was complete. At the end of the growing period, a total of 120 broilers from each experiment were weighed and …
