Molecular Characterization Of The Hspa1a Gene By Single-Strandconformation Polymorphism And Sequence Analysis In Holstein-Friesiancrossbred And Deoni Cattle Raised In India, Ramesha P. Kerekoppa, Akhila Rao, Marappa Basavaraju, Gujjar Ramachandra Geetha, Lakhsminarayan Krishnamurthy, Thota Vl Narasimha Rao, Dayal Nitai Das, Kataktalware Mukund
Turkish Journal of Veterinary & Animal Sciences
A group of proteins called heat shock proteins (HSPs) are synthesized during heat stress. They are expressed at high levels when exposed to stress. Polymorphism in HSPs has been reported to be associated with heat tolerance and reproductive performance in cattle. The present study aimed to investigate the polymorphisms in the coding region of the HSPA1A gene in Holstein-Friesian (HF) crossbreds and the Deoni breed of cattle. Genomic DNA was extracted from 94 animal blood samples and was subjected to polymerase chain reaction-single strand conformation polymorphism analysis, which revealed 14 band patterns in Deoni cattle and 8 band patterns in …
Association Between The Muc4 G.243a > G Polymorphism And Immune Andproduction Traits In Large White Pigs,
2015
TÜBİTAK
Association Between The Muc4 G.243a > G Polymorphism And Immune Andproduction Traits In Large White Pigs, Ying Liu, Xue Mei Yin, Ri Wei Xia, Yong Jiu Huo, Guo Qiang Zhu, Sheng Long Wu, Wen Bin Bao
Turkish Journal of Veterinary & Animal Sciences
PCR-RFLP analysis of intron 17 of the MUC4 gene was conducted in Large White pigs and associations with cytokine levels (IFN-?, IL-1ß, IL-4, IL-6, IL-8, IL-10, TGF-ß, and TNF-?), production traits (100-kg-weight day age, back-fat depth, and eye muscle depth), and reproductive performance within different litters (total number born, number born alive, number in live weaning litter) were analyzed with the aim of identifying genetic markers of disease resistance. The results revealed AA, AG, and GG genotypes, with an A243G mutation identified in the GG genotype. Correlation analysis indicated that IL-8 and IL-10 levels and 100-kg-weight day age were significantly …
The Effect Of Oleic And Linoleic Acids On In Vitro Bovineembryonic Development And Embryo Quality,
2015
TÜBİTAK
The Effect Of Oleic And Linoleic Acids On In Vitro Bovineembryonic Development And Embryo Quality, Tahi̇r Karaşahi̇n, Şevket Arikan
Turkish Journal of Veterinary & Animal Sciences
The present study was aimed at the investigation of the effects of oleic and linoleic acid on the in vitro development of Anatolian native black crossbred bovine embryos. Following the in vitro maturation and fertilization of oocytes, embryonic development stages were monitored using embryo culture medium supplemented with three different doses of oleic and linoleic acid (10, 100, and 1000 µM). Examination of the culture media 48 h after fertilization demonstrated that linoleic acid had no effect on embryo cleavage rates, while oleic acid produced a significant increase in cleavage rates (P < 0.01). It was determined that the highest dose of linoleic acid (1000 µM) and all three doses of oleic acid significantly increased the proportion of cultured oocytes developing to the morula-blastocyst stage (P < 0.001). As a result, while oleic acid significantly increased (P < 0.001) the proportion of embryos developing to the morula-blastocyst stage, linoleic acid had limited effects on embryonic development and quality. Thus, it was concluded that the supplementation of Anatolian native black crossbred bovine embryo cultures with oleic acid may induce positive effects on embryonic development and quality.
Influences Of Flunixin And Tenoxicam On The Pharmacokinetics Of Florfenicol In Lipopolysaccharide-Induced Endotoxemia,
2015
TÜBİTAK
Influences Of Flunixin And Tenoxicam On The Pharmacokinetics Of Florfenicol In Lipopolysaccharide-Induced Endotoxemia, Feri̇de Koç, Alptuğ Ati̇la, Emre Karakuş, Kami̇l Üney
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was to investigate the influences of flunixin (FM) and tenoxicam (TN) on the pharmacokinetics of florfenicol (FF) after coadministration in lipopolysaccharide (LPS)-induced endotoxemic rabbits. Fifteen male rabbits were used in this study. FF (20 mg/kg), FM (2 mg/kg), and TN (1 mg/kg) were coadministered via intravenous injection to the animals. The concentrations of FF were determined by high-performance liquid chromatography with diode-array detection from 0.08 to 12 h in plasma. The plasma concentration-time profile of FF was described using a noncompartmental open model. In this study, terminal half-life, area under the curve, mean residence time, …
Effects Of Dietary Supplementation Of Probiotics (Bacillus Subtilis, Bacillus Licheniformis, And Bacillus Natto) On Broiler Muscle Development And Meat Quality, Xianjian Zhou, Erhui Jin, Shenghe Li, Chenfang Wang, Enmei Qiao, Guozhong Wu
Turkish Journal of Veterinary & Animal Sciences
This paper aims to evaluate the effects of probiotics (Bacillus subtilis, Bacillus licheniformis, and Bacillus natto) on muscle development and meat quality of broilers. In this study, 480 broilers (1 day old) were randomly divided into 4 groups. Group A was fed with a basal diet. The basal diets of Group B, Group C, and Group D were respectively supplemented with 50 mg/kg chlortetracycline, 200 mg/kg probiotics, and 400 mg/kg probiotics. On day 21 and day 42, in each group, 18 randomly selected broilers were tested to determine muscle performances. On day 21, compared with Group A, Group C and …
Identification Of Simple Sequence Repeat Markers In The Dromedary(Camelus Dromedarius) Genome By Next-Generation Sequencing,
2015
TÜBİTAK
Identification Of Simple Sequence Repeat Markers In The Dromedary(Camelus Dromedarius) Genome By Next-Generation Sequencing, Monther Sadder, Hussein Migdadi, Ahmed Al-Haidary, Aly Okab
Turkish Journal of Veterinary & Animal Sciences
The availability of molecular markers in camels is limited. The aim of this study was to develop new simple sequence repeat (SSR) markers. Four breeds of pooled dromedary genome were sequenced at low coverage utilizing Roche and Illumina platforms. A total of 65,746 contigs, covering approximately 52 Mb (2316 contigs > 2 kb), were assembled. The partial genome revealed 613 SSR loci with a minimum number of 5 repeat units. Comparative chromosomal location for 60 camel loci was predicted against bovine genome assembly Baylor Btau_4.6.1/bosTau7. Ten markers (16.7%) returned matches with a >100 score and >80% identity. SSR abundance was 1 …
Temperature Range Analysis (T_{Max}) On Dorsal Surface Of Sporting Horses,
2015
TÜBİTAK
Temperature Range Analysis (T_{Max}) On Dorsal Surface Of Sporting Horses, Ewa Jodkowska, Krzysztof Dudek, Maria Soroko
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine temperature differences on the dorsal surface of 32 clinically healthy show jumping horses. Temperature was measured using a Thermovision 550 (FLIR) camera, determining maximum temperature (T_{max}). The horse?s body was divided into the following regions: neck, withers, cranial back, caudal back, loins, and hindquarters. Those regions were subdivided into middle, left, and right parts. Statistical methods used for calculations consisted of Wilcoxon?s signed rank test and Dunn?s post hoc comparison test. Temperatures on the dorsal surface ranged from 19.5 to 31.5 °C, with the lowest indicated in the withers area and the …
Concentrations Of Manganese, Iron, And Strontium In Bones Of Thedomestic Dog (Canis Lupus Familiaris),
2015
TÜBİTAK
Concentrations Of Manganese, Iron, And Strontium In Bones Of Thedomestic Dog (Canis Lupus Familiaris), Halina Budis, Elzbieta Kalisinska, Natalia Lanocha, Danuta Kosik_Bogacka
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine concentrations of manganese (Mn), iron (Fe), and strontium (Sr) in the cartilage, compact bone, spongy bone, and cartilage with adjacent compact bone of the domestic dog (Canis lupus familiaris). The experiment was carried out on bones from the hip joints of 24 dogs (dogs of 8 years) from northwestern Poland. The concentrations of Mn, Fe, and Sr were assessed by atomic absorption spectrophotometry. Fe concentration was the highest in the cartilage and compact bone. Mn concentration was the lowest in all three types of bone material and had a significantly lower concentration …
Prevalence Of Toxoplasma Gondii In Sheep Meats Purchased From Retailstores In Central Anatolia, Turkey,
2015
TÜBİTAK
Prevalence Of Toxoplasma Gondii In Sheep Meats Purchased From Retailstores In Central Anatolia, Turkey, Kader Yildiz, Fatma Çi̇ğdem Pi̇şki̇n, Armağan Erdem Ütük, Sami̇ Gökpinar
Turkish Journal of Veterinary & Animal Sciences
Toxoplasmosis is one of the most important foodborne parasitic diseases of humans. In particular, sheep muscles are significant sources of infection in the transmission of toxoplasmosis. Carnivorism is the most important transmission route for human populations. The aim of this study was to detect the prevalence of Toxoplasma gondii tissue cysts in sheep meats in retail stories of Turkey. A total of 250 boneless sheep meat samples were purchased from randomly selected retail stores in different locations of Ankara and Kırıkkale provinces of Turkey. The homogenized meat samples were centrifuged with Percoll dilutions. The tissue cysts were removed by pipette …
Estimating Allele Frequencies Of Some Hereditary Diseases In Holstein Cattlereared In Burdur Province, Turkey,
2015
TÜBİTAK
Estimating Allele Frequencies Of Some Hereditary Diseases In Holstein Cattlereared In Burdur Province, Turkey, Özgecan Korkmaz Ağaoğlu, Ali̇ Reha Ağaoğlu, Mustafa Saatci
Turkish Journal of Veterinary & Animal Sciences
The economics of dairy cattle breeding around the world has been significantly affected by the autosomal recessive hereditary diseases of bovine leukocyte adhesion deficiency (BLAD), factor XI deficiency (FXID), bovine citrullinemia (BC), and deficiency of uridine monophosphate synthase (DUMPS). The aim of this study was to determine BLAD, FXID, BC, and DUMPS alleles in Holstein cattle raised in Burdur Province in Turkey. This study involved taking blood samples randomly from 500 head of Holstein cattle at various dairy farms in different towns in Burdur Province. Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis was employed to identify cows …
Relationships Of Fatty Acid Group Contents In Milk And Reproductiveperformance In Holstein Cows,
2015
TÜBİTAK
Relationships Of Fatty Acid Group Contents In Milk And Reproductiveperformance In Holstein Cows, Jaromír Duchácek, Jan Beran, Martin Ptácek, Ludek Stádník, Monika Okrouhla, Renata Tousová, Martina Dolezalova
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to evaluate the relationships between milk fatty acid group contents and subsequent reproductive performance in Holstein cows. A total of 27 cows in the 1st to 4th lactations were included in the evaluation. Cows were divided into primiparous and multiparous groups. Daily milk yields increased in the first 8 weeks of lactation. Body condition score decreased until the third month of lactation. As the cows gradually recovered from the negative energy balance, the contents of saturated fatty acids (SFAs) increased, whereas the contents of monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs) decreased. …
Comparative Effects Of Sirolimus-Eluting Versus Bare Metal Stents On Neointimal Hyperplasia In Balloon-Injured External Iliac Artery Of Rabbits, Atul Patel, Pinesh Parikh, Deepak Patil, Anila Mathew, Suhas Lele, Mohan Rajapurkar, Pradip Patil, Akhilesh Kumar, Priti Vani
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to check effectiveness of drug-eluting stents for prevention of restenosis resulting from neointimal hyperplasia. Twenty-one sirolimus-eluting stents (SESs) and bare metal stents (BMSs; control) were compared by implantation into left and right balloon-injured external iliac arteries of New Zealand White rabbits, respectively. Under general anesthesia and angiography, using 3-mm coronary balloon catheters, stents were successfully deployed. Anticoagulant therapy was not given to any animal. Rabbits were allotted into three groups at 7, 14, and 21 days (7 rabbits in each group). Each stented vessel of sacrificed rabbits was subjected to a resin-embedding technique and …
Effects Of High-Level Chromium Methionine Supplementation In Lambs Fed A Corn-Based Diet On The Carcass Characteristics And Chemical Composition Of The Longissimus Muscle, Berenice Sanchez-Mendoza, Antonio Aguilar-Hernandez, Maria A. Lopez-Soto, Alberto Barreras, Alfredo Estrada-Angulo, Francisco Javier Monge Navarro, Noemi G. Torrentera, Richard A. Zinn, Alejandro Plascencia
Turkish Journal of Veterinary & Animal Sciences
Twenty-four male lambs (24.93 ± 0.93 kg) were used in order to evaluate the effects of chromium methionine (Cr) supplementation in high-energy finishing diets on the carcass characteristics and chemical composition of the longissimus muscle (LM). Treatments were 0.00, 0.60, 1.20, and 1.80 mg Cr/lamb daily. The experiment lasted 56 days. There were no effects of treatments on dry matter intake, feed to gain ratio, and final weight, which averaged 35.24 ± 1.01 kg. Supplemental Cr linearly decreased fat thickness enough to improve the estimated yield grade from 1.82 to 1.42 with no effect on the other carcass traits measured. …
Concentrations Of Nefa, Ss-Hba, Triglycerides, And Certain Blood Metabolitesin Healthy Colored Angora Goats During The Peripartum Period, Funda Eşki̇, İbrahi̇m Taşal, Mehmet Aki̇f Karsli, Sai̇t Şendağ, Bariş Atalay Uslu, Henrik Wagner, Axel Wehrend
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the changes in serum nonesterified fatty acid (NEFA), serum ß-hydroxybutyric acid (ß-HBA), triglycerides, Ca, Na, and other metabolites (bilirubin, glutamate dehydrogenase (GLDH)) in the blood of grazing, healthy goats at the time of parturition. Blood samples were taken weekly from the jugular vein of 11 goats, starting at week 2 antepartum (ap) until week 9 postpartum (pp). NEFA and ß-HBA concentrations increased from week 2 ap to 2 weeks pp. The increase in NEFA level was not significant; however, the ß-HBA levels were higher (P < 0.05) 2 weeks pp compared to the levels at 2 weeks ap. Triglycerides were recorded at maximum levels (P < 0.05) 2 weeks ap, with the lowest concentrations at 3 weeks pp. Bilirubin levels consistently increased up to 7 weeks pp, followed by a decrease. However, these changes were not significant. Similarly, GLDH activities increased until week 8 pp. A significant difference (P < 0.05) was recorded between the 1st week and 8th week pp. Ca and Na levels were lower during the 1st week pp and increased at 3 weeks pp. The results show that there are characteristic alterations of some metabolic blood parameters in goats around the time of parturition, which may be related to physiological changes.
Influence Of Individual Or Mixed Cellulase And Xylanase Mixture On In Vitro Rumen Gas Production Kinetics Of Total Mixed Rations With Different Maize Silage And Concentrate Ratios, Mona Mohamed Mohamed Yasseen Elghandour, Ahmed Eid Kholif, Ofelia Márquez-Molina, José Fernando Vázquez-Armijo, Anil Kumar Puniya, Abdelfattah Zeidan Mohamed Salem
Turkish Journal of Veterinary & Animal Sciences
This study assessed the effects of cellulase, xylanase, and their mixture (1:1, v/v) on in vitro rumen fermentation of five mixed rations with different maize silage (F) to concentrate (C) ratios (0F:100C, 25F:75C, 50F:50C, 75F:25C, 100F:0C). Samples were incubated using rumen inoculum from Brown Swiss cows fed ad libitum a total mixed ration of concentrate and alfalfa hay (1:1). Gas production (GP) was recorded after 2, 4, 6, 8, 10, 12, 24, 36, 48, and 72 h of incubation. Interaction effects (P < 0.0001) were observed between type of ration and enzyme for discrete lag time prior to GP. Ration type affected asymptotic GP (linear and quadratic, P < 0.0001) and in vitro GP at 24 h onward. Moreover, rations affected pH (linear and quadratic, P < 0.0001), dry matter degradability (quadratic, P = 0.05), metabolizable energy (linear, P = 0.038), short-chain fatty acids (linear, P = 0.0005), gas yield from truly digested dry matter at 24 h (linear, P = 0.0026), and microbial crude protein yield (linear, P = 0.0264). The most effective rations for in vitro GP during different times differed between different measured parameters. Enzyme administration was more effective when the ratio of maize silage was increased.
Evaluation Of Serum Enzyme Activities And Protein Fractions In Brucella-Infected Cows,
2015
TÜBİTAK
Evaluation Of Serum Enzyme Activities And Protein Fractions In Brucella-Infected Cows, Mohamed Fahmy Abou Elazab
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to evaluate potential changes in serum enzyme activities and protein fractions in Brucella-infected cows. A total of 1100 serum samples of dairy cows were screened for Brucella infection using the Rose Bengal test (RBT). Serum samples reacting positively to the RBT were subjected to indirect enzyme-linked immunosorbent assay for confirmation. Thirty serum samples from serologically positive cows and 30 from negative (healthy) cows were analyzed for alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatine kinase (CK), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), and gamma-glutamyl transferase (GGT) activities and total protein (TP) concentrations and serum protein …
Determination Of The Survival Levels Of Acid-Adapted Escherichia Coli O157:H7in Sucuk (Turkish-Type Fermented Sausage),
2015
TÜBİTAK
Determination Of The Survival Levels Of Acid-Adapted Escherichia Coli O157:H7in Sucuk (Turkish-Type Fermented Sausage), Fatma Öztürk, Abdulkadi̇r Halkman
Turkish Journal of Veterinary & Animal Sciences
This study aimed to determine the time taken for Escherichia coli O157:H7 to adapt to different types of acids and the effect of acid adaptation on the survival level of E. coli O157:H7 in sucuk (Turkish-type fermented sausage). E. coli O157:H7 cells were adapted to acids in a tryptic soy broth (TSB) set to pH 5.5 for 1-, 2-, 3-, and 4-h periods using hydrochloric, acetic, lactic, and citric acid. The acid tolerance of acid-adapted and nontreated cells was determined in TSB of pH 3.5 and then acid-adapted and nontreated E. coli O157:H7 (105 CFU/g) was added to sucuk. In …
Multiple-Locus Variable Number Of Tandem Repeat Analysis Assay As A Tool For The Epidemiological Study Of Brucella Canis In Korea, Kichan Lee, Ji-Yeon Kim, Sung-Il Kang, Hyang Keun Lee, Suk-Chan Jung, Moon Her
Turkish Journal of Veterinary & Animal Sciences
Multiple-locus variable number of tandem repeats analysis (MLVA) is useful for epidemiological investigations. In this study, MLVA-18 was used to assess the genetic and epidemiological relationships of Brucella canis isolates among a domestic dog and dogs from two breeding farms. MLVA-18 assay showed variation in copy numbers in 6 of the 18 loci. Interestingly, the YJ02-1 strain isolated from the pet dog from Yangju-si showed only one tandem repeat change in Bruce 09 locus as compared with that of NYJ-1 strain isolated from a dog from the Namyangju-si farm. The two strains were shown to have originated from the same …
Clinical And Radiographic Characterization Of Xenotransplantation Of Rat Bonemarrow-Derived Mesenchymal Stem Cells For Repair Of Radial Defects Of Rabbit, Betania Monteiro, Lukiya Favarato, Pablo Carvalho, Barbara Okano, Maria Antonia Menegatti, Alvaro Oliveira, Bianka Santos, Ricardo Del Carlo
Turkish Journal of Veterinary & Animal Sciences
This study aimed to evaluate the clinical and radiographic aspects of xenogenic transplantation of rat bone marrow-derived mesenchymal stem cells (MSCs) for the repair of radial bone defects of rabbit. Bone marrow derived MSCs collected from Wistar rats were expanded, differentiated, and labeled in the laboratory. In the fourth passage, the cultures were pelleted in aliquots of 107 cells/50 μL and transplanted in situ into the lesions. Thirty-six rabbits were used in the in vivo study. In all animals, defects were created by removing a bone segment of 1.0 cm in length in the right radius. The rabbits were divided …
Microencapsulated Organic Acid Blend With Mcfas Can Be Used As Analternative To Antibiotics For Laying Hens,
2015
TÜBİTAK
Microencapsulated Organic Acid Blend With Mcfas Can Be Used As Analternative To Antibiotics For Laying Hens, Sang In Lee, Hyun Soo Kim, Inho Kim
Turkish Journal of Veterinary & Animal Sciences
A total of 144 Hy-Line brown laying hens were used in a 10-week trial to evaluate the effects of a microencapsulated organic acid blend with medium chain fatty acids (MCFAs) on egg production, weight, quality, fecal microflora, and nutrient digestibility in the hens. The hens were divided into four groups and different dietary treatments were given to each group. The control group received no microencapsulated organic acid blend with MCFAs. The second group received 0.05%, the third group received 0.01%, and the fourth group received 0.2% of a microencapsulated organic acid blend with MCFAs. Laying hens fed on a microencapsulated …
