Molecular Characterization Of Complete Genome Of A Thai Highly Pathogenic Porcine Reproductive And Respiratory Syndrome Virus,
2014
Chulalongkorn University
Molecular Characterization Of Complete Genome Of A Thai Highly Pathogenic Porcine Reproductive And Respiratory Syndrome Virus, Tippawan Jantafong, Porntippa Lekcharoensuk
The Thai Journal of Veterinary Medicine
Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) causes severe outbreaks in swine leading to serious economic losses in swine industry in Thailand and other countries in Southeast Asia. However, information regarding whole genome characterization of HP-PRRSV isolate in this region is limited. This report describes sequencing and characterizations of the complete nucleotide sequence of a Thai HP-PRRSV isolate, designated HP/Thailand/19500LL/2010 (TH19500LL/10). The complete genomic sequence consists of 189-nt 5¢UTR, 14,982-nt protein-coding region containing 9 ORFs and 150-nt 3¢UTR. In addition, the full-length sequence of Thai HP-PRRSV isolate was compared with that of VR2332 and other HP-PRRSV strains. The …
Development Of One-Step Reverse Transcription Loop-Mediated Isothermal Amplification (Lamp) As A Screening Tool For Influenza A Viruses,
2014
Chulalongkorn University
Development Of One-Step Reverse Transcription Loop-Mediated Isothermal Amplification (Lamp) As A Screening Tool For Influenza A Viruses, Hein Min Tun, Trong Wisedchanwet, Manoosak Wongphatcharachai, Nutthawan Nonthabenjawan, Supasama Chaiyawong, Apiradee Theamboonlers, Yong Poovorawan, Alongkorn Amonsin
The Thai Journal of Veterinary Medicine
Influenza A virus is a major cause of influenza pandemics and can infect several host species including humans and animals. The objective of this study was to develop a one-step reverse transcription loop-mediated isothermal amplification assay (LAMP) for the detection of genetically diverse influenza A viruses from both human and animal hosts. First, a set of two inner and two outer primers were designed based on the conserved region of the matrix (M) gene of influenza A viruses. The amplification reaction was optimized at 63oC for 60 min and performed in a simple heat block. The amplicons could be visualized …
Assessment Of 2,4-Difluoroaniline Aquatic Toxicity Using A Zebrafish (Danio Rerio) Model,
2014
Chulalongkorn University
Assessment Of 2,4-Difluoroaniline Aquatic Toxicity Using A Zebrafish (Danio Rerio) Model, Crina Laura Moșneang, Adrian Grozea, Ion Oprescu, Eugenia Dumitrescu, Florin Muselin, Dénes Gál, Romeo T. Cristina
The Thai Journal of Veterinary Medicine
Bio-monitoring, in the control strategies for pollution, has several advantages over other tests in aquatic organisms. Bio-effects may link the bioavailability of the compounds of interest with their concentration at target organs and intrinsic toxicity. Potential harmful effects of 2,4-difluoroaniline (DFA), on a 96-hour acute static test, on adult zebrafish (Danio rerio) were followed. According to OECD 203: Fish Acute Toxicity Test, LC50 was determined using 70 adult zebrafish AB strain and 14 zebrafish as control group, to evaluate the effects and action at different concentrations of DFA. Obtained data were analyzed using Minitab Statistical Software as Goodness-of-Fit correlation tests. …
The Multidrug-Resistant Pseudomonas Aeruginosa Clinical Isolates From Dogs And Cats Expressed Three Multidrug Efflux Systems Simultaneously,
2014
Chulalongkorn University
The Multidrug-Resistant Pseudomonas Aeruginosa Clinical Isolates From Dogs And Cats Expressed Three Multidrug Efflux Systems Simultaneously, Kanchana Poonsuk, Rungtip Chuanchuen
The Thai Journal of Veterinary Medicine
The objective of this study was to examine the expression of 4 clinically-important Mex systems including MexAB-OprM, MexCD-OprJ, MexEF-OprN and MexXY in the Pseudomonas aeruginosa clinical isolates from dogs and cats. The isolates exhibited high level of resistance to multiple antibiotics clinically important. All of them simultaneously overexpressed up to three different Mex systems, including MexAB-OprM, MexEF-OprN and MexXY as determined by RT-PCR. None of the isolates overexpressed MexCD-OprJ. Expression of mexF was measured by using quantitative real-time RT-PCR. Transcription level of mexF varied (i.e. 4-219 fold) but was at least 4 fold higher than that of PAO1. DNA sequence …
Stimulatory Effects Of Low Intensity Laser Therapy On The Healing Of Rabbit Tibial Defects,
2014
Chulalongkorn University
Stimulatory Effects Of Low Intensity Laser Therapy On The Healing Of Rabbit Tibial Defects, Hoai Nam Nguyen, Peerapol Sukon, Sirikachorn Tangkawattana, Pipop Sutthiprapaporn, Pornpop Rattana-Arpha, Naruepon Kampa
The Thai Journal of Veterinary Medicine
The present study was to investigate the effects of low intensity laser therapy (LILT) on the healing of rabbit tibial defects. Circular defects were perforated at the medial site of tibial crests on both hind legs of 18 male New Zealand White rabbits. The defects were randomly allocated into 3 groups (n=12): 1) defects irradiated with LILT, at 4 J/cm2 (LS4); 2) defects irradiated with LILT, at 20 J/cm2 (LS20), and 3) defects receiving sham treatment (control group). Irradiation began 24h after surgery, and repeated every 48 h, with a total of 8 sessions. In the 3rd and 6th weeks …
Quantitative Method For Detecting Vibrio Species Using Bio-Theta Doxtm System,
2014
Chulalongkorn University
Quantitative Method For Detecting Vibrio Species Using Bio-Theta Doxtm System, Shouichi Tanno, Naoki Fukui, Yukihiro Utaka, Saburo Ohkawa, Yukio Morita, Sumalee Boonmar
The Thai Journal of Veterinary Medicine
This is the first report of a quantitative method for detecting Vibrio species using the Bio-Theta DOXTM system. We assessed 19 strains of 9 Vibrio species and 22 strains of 22 non-Vibrio species and found that the positive rate in the medium-concentration (103 cfu/ml) and low-concentration (10 cfu/ml) samples of the 19 Vibrio samples was 100% and 89.5% (17/19), respectively. A few low-concentration samples of V. alginolyticus and V. vulnificus were negative. All Vibrio strains displayed good linear calibration curves for detection time vs bacterial count (r>0.94). The negative rate of the high-concentration (106 cfu/ml) and medium-concentration (103cfu/ml) samples …
Ultrasound Diagnosis,
2014
Chulalongkorn University
Ultrasound Diagnosis, Phiwipha Kamonrat
The Thai Journal of Veterinary Medicine
No abstract provided.
Evaluation Of Porcine Ipec-J2 Cell Line Growth Rate And Immune Response To E. Coli (0111:B4) Lps,
2014
University of Arkansas, Fayetteville
Evaluation Of Porcine Ipec-J2 Cell Line Growth Rate And Immune Response To E. Coli (0111:B4) Lps, Xiaofan Wang
Graduate Theses and Dissertations
The objective of this study was to evaluate the intestinal porcine epithelial cell-jejunum 2 (IPEC-J2) cell line as a model to study the innate immune function of live pigs. Growth rates of IPEC-J2 cells in T-75 flasks and 96-well plates were evaluated using a hemocytometer and spectrophotometer for cell quantity measurements to determine growth rate and doubling time. Growth rates of IPEC-J2 cells in T-75 flasks and 96-well plates were 0.4016 and 0.2851 times of doubling/day respectively with a doubling time of 1.73 d and 2.43 d, respectively. Confluent IPEC-J2 monolayers were tested at five time intervals (0, 1, 2, …
Glucose Significantly Enhances Enterotoxigenic
Escherichia Coli Adherence To Intestinal Epithelial Cells
Through Its Effects On Heat-Labile Enterotoxin
Production,
2014
University of Nebraska-Lincoln
Glucose Significantly Enhances Enterotoxigenic Escherichia Coli Adherence To Intestinal Epithelial Cells Through Its Effects On Heat-Labile Enterotoxin Production, Prageeth Wijemanne, Rodney A. Moxley
School of Veterinary and Biomedical Sciences: Faculty Publications
The present study tested whether exposure of enterotoxigenic Escherichia coli (ETEC) to glucose at different concentrations in the media results in increased bacterial adherence to host cells through increased heat-labile enterotoxin (LT) production, thereby suggesting the effects are physiological. Porcine-origin ETEC strains grown in Casamino acid yeast extract medium containing different concentrations of glucose were washed and inoculated onto IPEC-J2 porcine intestinal epithelial cells to test for effects on adherence and host cell cAMP concentrations. Consistent with previous studies, all LT+ strains had higher ETEC adherence to IPEC-J2 cells than did LT2 strains. Adherence of the LT2 but not the …
Copy Number Variation In The Horse Genome,
2014
Texas A&M University
Copy Number Variation In The Horse Genome, Sharmila Ghosh, Zhipeng Qu, Pranab J. Das, Erica Fang, Rytis Juras, E. Gus Cothran, Sue Mcdonell, Daniel G. Kenney, Teri L. Lear, David L. Adelson, Bhanu P. Chowdhary, Terje Raudsepp
Maxwell H. Gluck Equine Research Center Faculty Publications
We constructed a 400K WG tiling oligoarray for the horse and applied it for the discovery of copy number variations (CNVs) in 38 normal horses of 16 diverse breeds, and the Przewalski horse. Probes on the array represented 18,763 autosomal and X-linked genes, and intergenic, sub-telomeric and chrY sequences. We identified 258 CNV regions (CNVRs) across all autosomes, chrX and chrUn, but not in chrY. CNVs comprised 1.3% of the horse genome with chr12 being most enriched. American Miniature horses had the highest and American Quarter Horses the lowest number of CNVs in relation to Thoroughbred reference. The Przewalski horse …
Growing South Dakota (Fall 2014),
2014
South Dakota State University
Growing South Dakota (Fall 2014), College Of Agriculture &. Biological Sciences
Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)
[Page] 2 Abundant Opportunities: Bright Ag & Bio Career Outlook Fuels Enhancements
[Page] 4 Future Focus: New Institute Supports Efforts In Science, Technology, Engineering, Math
[Page] 7 Grant Funding Updates
[Page] 8 Profiles in Leadership: Engaging Students Utilizing Problem-Based Learning
[Page] 9 Profiles in Leadership: The Value Of Experience-Based Learning
[Page] 10 Campus News
[Page] 12 Steps To Ensure Student Success: Support Fostered In Living-Learning Communities
[Page] 14 New Student Advising Model Gets High Marks
[Page] 15 Student To Student: Ag Bio Ambassadors Relish Role As Front Line Recruiters
[Page] 16 Remembering Dr. Robert Pengra: SDSU Alums Establish Tribute Scholarship …
Efficacy Of Short Novel Antimicrobial And Anti-Inflammatory Peptides In A Mouse Model Of Methicillin-Resistant Staphylococcus Aureus (Mrsa) Skin Infection,
2014
Purdue University
Efficacy Of Short Novel Antimicrobial And Anti-Inflammatory Peptides In A Mouse Model Of Methicillin-Resistant Staphylococcus Aureus (Mrsa) Skin Infection, Mohamed F. Mohamed, Mohamed N. Seleem
Department of Comparative Pathobiology Faculty Publications
The therapeutic efficacy of two novel short antimicrobial and anti-inflammatory peptides (RR and RRIKA) was evaluated in a mouse model of staphylococcal skin infection. RR (2%) and RRIKA (2%) significantly reduced the bacterial counts and the levels of proinflammatory cytokines, tumor necrosis factor (TNF)-α, and interleukin (IL)-6, in methicillin-resistant Staphylococcus aureusUSA 300-0114 skin lesions. Furthermore, the combined therapy of RRIKA (1%) and lysostaphin (0.5%) had significantly higher antistaphylococcal and anti-inflammatory activity compared to monotherapy. This study supports the potential use of these peptides for topical treatment of methicillin-resistant Staphylococcus aureus skin infections.
Review Of The Pathogenesis And Treatment Of Acute Spinal Cord Injury And Investigation Into The Use Of Urine 3-Hpma As A Novel Biomarker Of Secondary Injury After Acute Spinal Cord Injury In The Dog,
2014
Purdue University
Review Of The Pathogenesis And Treatment Of Acute Spinal Cord Injury And Investigation Into The Use Of Urine 3-Hpma As A Novel Biomarker Of Secondary Injury After Acute Spinal Cord Injury In The Dog, Andrea Sangster
Open Access Theses
Acute spinal cord injury (ASCI) has two pathophysiological stages of injury: the primary injury and the secondary injury cascade. Primary injury includes the initial or mechanical insult to the spinal cord. Secondary injury is a cascade of biochemical events that propagates damage of adjacent, healthy tissue increasing the overall volume of spinal cord tissue that is affected. Acrolein is a toxic byproduct of lipid peroxidation produced during secondary injury. A metabolite of acrolein-glutathione adduct found in urine, 3-HPMA, has recently been shown to increase after spinal cord injury in a rat model. The aim of our study was to apply …
The Effects Of Heat Stress On Immunity In Laying Hens And Dairy Cattle,
2014
Purdue University
The Effects Of Heat Stress On Immunity In Laying Hens And Dairy Cattle, Rebecca Ann Strong
Open Access Theses
With the increase in global climate change and the population growth driving the high demand for additional food production, heat stress (HS) is a major concern in the livestock industry across all species. Animals experience HS when exposed to high environmental temperatures outside their thermal neutral zone. The level of the effects can vary due to the length and intensity of HS to which the animal is exposed to. In experiment one, laying hens with access to cooled perches during HS had a lower heterophil to lymphocyte ratio compared to the control hens after 4 h of acute heat stress, …
Prediction Of Parturition And Dystocia In Holstein-Friesian Cattle, And Cesarean Section In Dystocic Beef Cattle,
2014
Purdue University
Prediction Of Parturition And Dystocia In Holstein-Friesian Cattle, And Cesarean Section In Dystocic Beef Cattle, Wen Han Mark Hiew
Open Access Dissertations
Dystocia is a major problem in the dairy industry as it causes livestock and economic loss. It is more frequently seen in primiparous cattle compared to their multiparous counterparts due to their smaller stature and the slow maturation of pelvic dimensions. In some instances, human intervention of the parturition process is imperative to avoid pain, injury, and mortality of the neonate and the dam. The ability to accurately predict dystocia and the time of parturition will ensure that timely assistance can be given to animals that are of high risk of dystocia.^ The present thesis contains four studies that explore …
Development Of A Fatal Noncompressible Truncal
Hemorrhage Model With Combined Hepatic And Portal
Venous Injury In Normothermic Normovolemic Swine,
2014
University of Nebraska Medical Center, Omaha
Development Of A Fatal Noncompressible Truncal Hemorrhage Model With Combined Hepatic And Portal Venous Injury In Normothermic Normovolemic Swine, Ujwal R. Yanala, Jason M. Johanning, Iraklis I. Pipinos, Gustavo F. Larsen, William H. Velander, Mark A. Carlson
William H. Velander Publications
Noncompressible truncal hemorrhage and brain injury currently account for most early mortality of warfighters on the battlefield. There is no effective treatment for noncompressible truncal hemorrhage, other than rapid evacuation to a surgical facility. The availability of an effective field treatment for noncompressible truncal hemorrhage could increase the number of warfighters salvaged from this frequently-lethal scenario. Our intent was to develop a porcine model of noncompressible truncal hemorrhage with a ,50% one-hour mortality so that we could develop new treatments for this difficult problem. Normovolemic normothermic domestic swine (barrows, 3 months old, 34–36 kg) underwent one of three injury types …
The Distribution Pattern Of Halicephalobus Gingivalis In A Horse Is Suggestive Of A Haematogenous Spread Of The Nematode,
2014
University of Copenhagen, Denmark
The Distribution Pattern Of Halicephalobus Gingivalis In A Horse Is Suggestive Of A Haematogenous Spread Of The Nematode, Christina Henneke, Anna Jespersen, Stine Jacobsen, Martin K. Nielsen, Fintan Mcevoy, Henrik E. Jensen
Veterinary Science Faculty Publications
The majority of Halicephalobus gingivalis-infections in horses have been fatal and are usually not diagnosed before necropsy. Therefore, knowledge about the nematode and the pathogenesis of infection in horses is limited. This has resulted in an on-going discussion about the port of entry and subsequent dissemination of H. gingivalis within the host. The present case of H. gingivalis-infection in a horse was diagnosed ante mortem. Post mortem findings, the distribution pattern of H. gingivalis nematodes in the brain, a high prevalence of inflammation in close relation to blood vessels, and the presence of the nematode in multiple organs …
A Rational Framework For Evaluating The Next Generation Of Vaccines Against Mycobacterium Avium Subspecies Paratuberculosis,
2014
USDA-ARS
A Rational Framework For Evaluating The Next Generation Of Vaccines Against Mycobacterium Avium Subspecies Paratuberculosis, John P. Bannantine, Murray E. Hines Ii, Luiz E. Bermudez, Adel M. Talaat, Srinand Sreevatsan, Judith R. Stabel, Yung-Fu Chang, Paul M. Coussens, Raúl G. Barletta, William C. Davis, Desmond M. Collins, Yrjö T. Gröhn, Vivek Kapur
School of Veterinary and Biomedical Sciences: Faculty Publications
Since the early 1980s, several investigations have focused on developing a vaccine against Mycobacterium avium subspecies paratuberculosis (MAP), the causative agent of Johne's disease in cattle and sheep. These studies used whole-cell inactived vaccines that have proven useful in limiting disease progression, but have not prevented infection. In contrast, modified live vaccines that invoke a Th1 type immune response, may improve protection against infection. Spurred by recent advances in the ability to create defined knockouts in MAP, several independent laboratories have developed modified live vaccine candidates by transcriptional mutation of virulence and metablolic genes in MAP. In order to accelerate …
Sequence-Based Antigenic Change Prediction By A Sparse Learning Method Incorporating Co-Evolutionary Information.,
2014
Mississippi State University
Sequence-Based Antigenic Change Prediction By A Sparse Learning Method Incorporating Co-Evolutionary Information., Jialiang Yang, Tong Zhang, Xiu-Feng Wan
CVM Publications
Rapid identification of influenza antigenic variants will be critical in selecting optimal vaccine candidates and thus a key to developing an effective vaccination program. Recent studies suggest that multiple simultaneous mutations at antigenic sites accumulatively enhance antigenic drift of influenza A viruses. However, pre-existing methods on antigenic variant identification are based on analyses from individual sites. Because the impacts of these co-evolved sites on influenza antigenicity may not be additive, it will be critical to quantify the impact of not only those single mutations but also multiple simultaneous mutations or co-evolved sites. Here, we developed and applied a computational method, …
An Evaluation Of A Liquid Antimicrobial (Sal Curb®) For Reducing The Risk Of Porcine Epidemic Diarrhea Virus Infection Of Naïve Pigs During Consumption Of Contaminated Feed,
2014
South Dakota State University
An Evaluation Of A Liquid Antimicrobial (Sal Curb®) For Reducing The Risk Of Porcine Epidemic Diarrhea Virus Infection Of Naïve Pigs During Consumption Of Contaminated Feed, Scott Dee, Casey Neill, Travis Clement, Jane Christopher-Hennings, Eric A. Nelson
Veterinary and Biomedical Sciences Faculty Publications
Background: Since its initial detection in May 2013, porcine epidemic diarrhea virus (PEDV) has spread rapidly throughout the US swine industry. Recently, contaminated feed was confirmed as a vehicle for PEDV infection of naïve piglets. This research provides in vivo data supporting the ability of a liquid antimicrobial product to reduce this risk.
Results: Sal CURB® (Kemin Industries, Des Moines, IA, USA) is a FDA-approved liquid antimicrobial used to control Salmonella contamination in poultry and swine diets. To test its effect against PEDV, Sal CURB®-treated feed was spiked with a stock isolate of PEDV (Ct = 25.22), which PEDV-naïve piglets …
